CN110563154A - microbial starting method for biological aerated filter - Google Patents

microbial starting method for biological aerated filter Download PDF

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Publication number
CN110563154A
CN110563154A CN201910677131.6A CN201910677131A CN110563154A CN 110563154 A CN110563154 A CN 110563154A CN 201910677131 A CN201910677131 A CN 201910677131A CN 110563154 A CN110563154 A CN 110563154A
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biological aerated
aerated filter
zeolite
microbial
nutrient
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赵皓翰
倪康祥
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GUANGDONG TROTH WATER EQUIPMENT Ltd
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GUANGDONG TROTH WATER EQUIPMENT Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C02F3/105—Characterized by the chemical composition
    • C02F3/107—Inorganic materials, e.g. sand, silicates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/38—Organic compounds containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a microorganism starting method of an aeration biological filter, which comprises the steps of carrying out enrichment culture on nitrifying sludge by using a nutrient and trace elements, controlling pH and ammonia nitrogen concentration to improve enrichment culture efficiency, and carrying out ammonia nitrogen saturation on zeolite filler to improve adhesion to nitrifying bacteria; the treated sludge is inoculated to the zeolite filler, and the biofilm formation of the nitrifying bacteria biofilm can be completed within 2-3 days, namely the microbial start of the biological aerated filter is completed, the ammonia nitrogen removal capability is strong, the starting method can be used for the initial stage of the biological aerated filter, also can be used for backwashing and restarting after long-time water cut-off, and the shutdown interval time is short.

Description

Microbial starting method for biological aerated filter
Technical Field
the invention relates to the technical field of sewage aeration biological treatment, in particular to a microbial start-up method for an aeration biological filter.
Background
a Biological Aerated Filter (BAF) for urban sewage is a water treatment device integrating biological oxidation treatment and deep filtration of sewage, and is mainly applied to advanced treatment of low-concentration sewage and pretreatment of micro-polluted source water, such as advanced treatment of urban domestic sewage and low-concentration industrial sewage, pretreatment of source water and the like. As an effective sewage treatment process, the biological aerated filter is emphasized by the characteristics of good water outlet effect, stable operation, convenient management, small occupied area and the like. However, in engineering application, the biological aerated filter basically adopts a single filler, so that the biological aerated filter has relatively single function and cannot efficiently remove various target pollutants.
The biological aerated filter which applies the most extensive common ceramsite or volcanic filler has good COD removing capability, but the ammonia nitrogen removing capability is limited, and the ammonia nitrogen of the sewage treated by the biological aerated filter can still exceed the standard in general. Therefore, the black and odorous water body with low C/N ratio and the domestic sewage are difficult to reach the standard quickly.
The generation period of the nitrifying bacteria is much longer than that of heterotrophic bacteria, the growth and propagation speed is slow, the yield is low, if organic pollutants (COD) in the inlet water greatly exceed ammonia nitrogen when the biofilm formation of the aeration biological filter is started, the heterotrophic bacteria propagate in large quantity and dominate in competition with the nitrifying bacteria, and the heterotrophic bacteria gradually become dominant bacteria, so that the nitrification efficiency of the reactor is reduced. The membrane hanging efficiency of the nitrifying bacteria has great influence on the affinity, the specific surface area and the like of the filter material to the bacteria.
Disclosure of Invention
in order to overcome the defects of the prior art, the invention aims to provide a microbial start-up method for a biological aerated filter. The starting method is fast in biofilm formation, only takes 5-7 days to realize enrichment culture and biofilm formation starting of the nitrobacteria.
The purpose of the invention is realized by adopting the following technical scheme:
The invention provides a microbial start-up method of a biological aerated filter, which comprises the following steps:
1) sludge treatment: adding a nutrient and trace elements into nitrified sludge of an urban domestic sewage plant, and performing aerobic aeration; the sludge concentration is 1500-2500 mg/L; the trace elements comprise manganese, zinc, cobalt, boron, molybdenum, copper and nickel; adjusting the pH value to 8.0-9.0, controlling the ammonia nitrogen concentration to 80-100mg/L and the temperature to 30-35 ℃, and carrying out enrichment culture on nitrifying bacteria in the sludge;
2) Adsorption saturation of zeolite in a denitrification layer: filling water into the denitrification layer with zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 80-120mg/L, namely the zeolite is fully adsorbed at the ammonia nitrogen equilibrium concentration;
3) inoculation: inoculating the sludge treated in the step 1) into the denitrification layer treated in the step 2), adding a nutrient, controlling the ammonia nitrogen concentration to be 50-100mg/L, adjusting the pH to be 7.5-8.5, and carrying out aerobic culture for several days to obtain a biofilm of nitrobacteria;
the nutrient comprises ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous salt, sodium salt and magnesium salt, wherein the total proportion of the ammonium sulfate and the sodium bicarbonate in the nutrient is 76-87 wt%.
Further, the ferrous salt is ferrous sulfate, the sodium salt is sodium chloride, and the magnesium salt is magnesium sulfate; ferrous salt accounts for 3-5 wt% of the nutritional agent, and sodium salt accounts for 4-6 wt% of the nutritional agent; magnesium salt accounts for 3-5 wt% of the nutritional agent.
Further, in the step 1), the trace element is MnSO4·4H2O、ZnSO4·7H2O、CoCl2·6H2O、H3BO4、Na2MoO4·2H2O、CuSO4·5H2o and NiCl2·6H2O composition; wherein, Mn: the mol ratio of Zn, Co, B, Mo, Cu and Ni is 20-25%, 15-20%, 10-15%, 8-15%, 5-10% and 5-10%.
Further, in the step 1), the weight ratio of the trace elements to the nutrient is 2-6: 100.
further, in step 1), sodium carbonate or sodium bicarbonate is used to adjust the pH.
Further, in the step 1), the culture is carried out until the number of nitrifying bacteria is 106-108one/mL.
Further, in step 2), the zeolite is natural zeolite or activated zeolite.
Further, in the step 2), the natural zeolite has the particle size of 3-5mm, the porosity of 50-60% and the specific surface area of 50-100m2/g；SiO2/Al2O3the ratio is 3.5-4.5, and the cation exchange capacity is 150-200 mmol/g.
Further, in the step 2), the particle size of the activated zeolite is 3-5mm, the porosity is 60% -75%, and the specific surface area is 120-200m2/g；SiO2/Al2O3The ratio is less than 4.5, and the cation exchange capacity is 250-400 mmol/g.
Further, in the step 3), the dissolved oxygen amount of aerobic culture is 4.0-6.0 mg/L.
Compared with the prior art, the invention has the beneficial effects that:
The microorganism starting method of the biological aerated filter provided by the invention has the advantages that nitrobacteria in the nitrified sludge are quickly enriched, and a denitrification layer taking zeolite as a filler carrier is saturated so as to improve the attachment capacity of the nitrobacteria; and (3) quickly forming a film on the filler carrier under the condition of proper pH environment and inorganic salt nutrient, thereby obtaining the microbial filter with higher ammonia nitrogen treatment capability and stable treatment effect.
The microorganism starting method of the biological aerated filter provided by the invention is characterized in that aerobic aeration is carried out on sludge containing nitrobacteria, inorganic salt nutrient and trace elements are added for enrichment culture, so that the nitrobacteria are rapidly propagated and enriched under the action of proper pH value, nitrogen sulfur phosphorus, sodium magnesium potassium ferrous ions and manganese zinc cobalt boron molybdenum copper and nickel trace elements, and the nitrobacteria number of the nitrosludge can be cultured to 10 through a culture period of 3-5 days6-108The sludge concentration can be increased by 3000-4000 mg/L.
According to the microbial start-up method for the biological aerated filter, enrichment culture and biofilm formation of nitrobacteria can be completed within 5-7 days by separately enriching nitrifying bacteria in sludge, saturating zeolite serving as a filler carrier and then performing biofilm formation, the strain biofilm formation quality is good, the treatment efficiency of ammonia nitrogen is stable, and the method can be used for starting up the biological filter at the initial stage and restarting the biological filter after water supply is cut off.
drawings
FIG. 1 is a photomicrograph of zeolite surface-coated microorganisms of example 3.
Detailed Description
The present invention is further described with reference to the accompanying drawings and the detailed description, and it should be noted that, in the present invention, the embodiments or technical features described below may be arbitrarily combined to form a new embodiment without conflict.
in the following embodiments, the content of ammonia nitrogen is NH4 +The amount of nitrogen present in the form.
The following are specific examples of the present invention, and raw materials, equipments and the like used in the following examples can be obtained by purchasing them unless otherwise specified.
A microbial start-up method for a biological aerated filter comprises the following steps:
1) Sludge treatment: adding a nutrient and trace elements into nitrified sludge of an urban domestic sewage plant, and performing aerobic aeration; the sludge concentration is 1500-2500 mg/L; the trace elements comprise manganese, zinc, cobalt, boron, molybdenum, copper and nickel; adjusting the pH value to 8.0-9.0, controlling the ammonia nitrogen concentration to 80-100mg/L and the temperature to 30-35 ℃, and carrying out enrichment culture on nitrifying bacteria in the sludge;
2) Adsorption saturation of zeolite in a denitrification layer: filling water into the denitrification layer with zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 80-120mg/L, namely the zeolite is fully adsorbed at the ammonia nitrogen equilibrium concentration;
3) inoculation: inoculating the sludge treated in the step 1) into the denitrification layer treated in the step 2), adding a nutrient, controlling the ammonia nitrogen concentration to be 50-100mg/L, adjusting the pH to be 7.5-8.5, and carrying out aerobic culture for several days to obtain a biofilm of nitrobacteria;
The nutrient comprises ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous salt, sodium salt and magnesium salt, wherein the total proportion of the ammonium sulfate and the sodium bicarbonate in the nutrient is 76-87 wt%.
the starting method is not only suitable for the initial starting of the biological aerated filter, but also suitable for the biofilm culturing starting after the backwashing.
example 1:
A method for starting the microbes in aerating biologic filter pool features that the microbes in aerating biologic filter pool are upward flowing and the height between bottom and water inlet and air inlet tubes is 0.2 mCobblestones with the grain diameter of 15-20mm are used as a bearing layer; the upper part of the supporting layer is filled with 4.0 m of natural zeolite as a denitrified layer which is used as a biological filler of nitrobacteria. Wherein the natural zeolite has particle diameter of 3-5mm, porosity of 50%, and specific surface area of 50m2/g； SiO2/Al2O3the ratio is 4.3, and the cation exchange capacity is 150 mmol/g;
the microbial starting method of the biological aerated filter comprises the following steps:
1) Sludge treatment: adding a nutrient and trace elements into nitrified sludge liquid of an urban domestic sewage plant, and aerating by aerobic and stuffy aeration; the concentration of the sludge liquid is 2000 mg/L; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate in a mass ratio of 45: 40: 5: 3: 4: 3; the trace elements include MnSO4·4H2O、ZnSO4·7H2O、CoCl2·6H2O、H3BO4、Na2MoO4·2H2O、 CuSO4·5H2O and NiCl2·6H2O composition; wherein, Mn: zn, Co, B, Mo, Cu and Ni in the molar ratio of 20-25%, 15-20%, 10-15%, 8-15%, 5-10% and 5-10%; the weight ratio of the trace elements to the nutrient is 2: 100, respectively; adding sodium carbonate every 2h to adjust pH to 8.0-8.4, adding ammonium sulfate every 2h to control ammonia nitrogen concentration to 80-100mg/L, controlling temperature of nitrified sludge liquid to 30 deg.C by electric heating, and performing enrichment culture on nitrifying bacteria in the sludge liquid for 3 days, wherein the number of nitrifying bacteria reaches 2 × 106The sludge concentration in the sludge liquid reaches 4000 mg/L;
2) Adsorption saturation of zeolite in a denitrification layer: filling water into a denitrification layer taking zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 100mg/L, namely the zeolite is saturated in the ammonia nitrogen equilibrium concentration;
3) Inoculation: inoculating the sludge liquid treated in the step 1) into the denitrified layer treated in the step 2), adding a nutrient, adding ammonium sulfate at intervals to control the ammonia nitrogen concentration to be 50-100mg/L, and adding sodium bicarbonate until the pH value is 8.0-8.5; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate in a mass ratio of 45: 40: 5: 3: 4: 3; aerobic culture is carried out for 3 days, the zeolite filler is taken out to find that the surface has thicker mucosa and a large amount of microorganisms are observed under a microscope, namely, the rapid film hanging of the nitrobacteria is realized;
4) Water test operation: and continuously feeding the water into an aerated biological filter according to 30%, 60% and 100% of the designed load water amount, monitoring the ammonia nitrogen value of inlet and outlet water, and finishing the start of the BAF microorganisms after the ammonia nitrogen value of the outlet water stably reaches the standard.
example 2:
A microorganism start method of a biological aerated filter is characterized in that microorganisms in the biological aerated filter are upflow biological aerated filters, cobblestones with the height of 0.2 meter and the grain diameter of 15-20mm are paved above water inlet and air inlet distribution pipes at the bottom of the biological aerated filter to serve as a bearing layer; the upper part of the supporting layer is filled with 4.5 m of natural zeolite as a denitrified layer which is used as a biological filler of nitrobacteria. Wherein the natural zeolite has particle diameter of 3-5mm, porosity of 65%, and specific surface area of 120m2/g；SiO2/Al2O3The ratio is 4.2, and the cation exchange capacity is 250 mmol/g;
The microbial starting method of the biological aerated filter comprises the following steps:
1) Sludge treatment: adding nutrient and trace elements into nitrified sludge liquid of an urban domestic sewage plant, wherein the dissolved oxygen amount is 6.0mg/L, and carrying out aerobic stuffy aeration; the concentration of the sludge liquid is 2000 mg/L; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate, and the mass ratio of the nutrient to the sodium chloride is 40: 40: 5: 5: 5: 6; the trace element is composed of MnSO4·4H2O、ZnSO4·7H2O、CoCl2·6H2O、H3BO4、Na2MoO4·2H2O、CuSO4·5H2o and NiCl2·6H2o composition; wherein, Mn: zn, Co, B, Mo, Cu and Ni in the molar ratio of 20-25%, 15-20%, 10-15%, 8-15%, 5-10% and 5-10%; the weight ratio of the trace elements to the nutrient is 4: 100, respectively; adding sodium carbonate every 2h to adjust pH to 8.0-8.4, adding ammonium sulfate every 2h to control ammonia nitrogen concentration to 80-100mg/L, and electrically heatingthe temperature of the nitrifying sludge liquid is controlled to be 35 ℃, the nitrifying bacteria in the sludge liquid are subjected to enrichment culture for 4 days, and the quantity of the nitrifying bacteria reaches 8 multiplied by 106The sludge concentration in the sludge liquid reaches 4500 mg/L;
2) adsorption saturation of zeolite in a denitrification layer: filling water into a denitrification layer taking zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 100mg/L, namely the zeolite is saturated in the ammonia nitrogen equilibrium concentration;
3) inoculation: inoculating the sludge liquid treated in the step 1) into the denitrified layer treated in the step 2), adding a nutrient, and controlling the content of dissolved oxygen to be 6.0 mg/L; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate, and the mass ratio of the nutrient to the sodium chloride is 40: 40: 5: 5: 5: 5; adding ammonium sulfate at intervals to control the ammonia nitrogen concentration to be 50-100mg/L, adding sodium bicarbonate until the pH value is 8.0-8.5, carrying out aerobic culture for 3 days, taking out the zeolite filler to find that thicker mucosa exists on the surface and observing under a microscope to find a large number of microorganisms, namely realizing the rapid biofilm formation of nitrobacteria;
4) Water test operation: and continuously feeding the water into an aerated biological filter according to 30%, 60% and 100% of the designed load water amount, monitoring the ammonia nitrogen value of inlet and outlet water, and finishing the start of the BAF microorganisms after the ammonia nitrogen value of the outlet water stably reaches the standard.
Example 3:
A microorganism start method of a biological aerated filter is characterized in that microorganisms in the biological aerated filter are upflow biological aerated filters, cobblestones with the height of 0.2 m and the grain diameter of 15-20mm are paved above water inlet and air inlet distribution pipes at the bottom of the biological aerated filter to serve as a bearing layer; the upper part of the supporting layer is filled with 4.5 m of activated zeolite as a denitrified layer which is used as a biological filler of nitrobacteria; wherein the activated zeolite has a particle size of 3-5mm, a porosity of 75%, and a specific surface area of 180m2/g；SiO2/Al2O3The ratio is 4.0, and the cation exchange capacity is 380 mmol/g;
the microbial starting method of the biological aerated filter comprises the following steps:
1) Sludge treatment: adding nutrient and trace elements into nitrified sludge liquid of an urban domestic sewage plant, wherein the dissolved oxygen amount is 5.0mg/L, and carrying out aerobic stuffy aeration; dirtThe concentration of the mud solution is 2000 mg/L; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate, and the mass ratio of the nutrient to the sodium chloride is 36: 42: 8: 4: 6: 4; the trace element is composed of MnSO4·4H2O、ZnSO4·7H2O、CoCl2·6H2O、H3BO4、Na2MoO4·2H2O、CuSO4·5H2O and NiCl2·6H2o composition; wherein, Mn: zn, Co, B, Mo, Cu and Ni in the molar ratio of 20-25%, 15-20%, 10-15%, 8-15%, 5-10% and 5-10%; the weight ratio of the trace elements to the nutrient is 6: 100, respectively; adding sodium carbonate every 2h to adjust pH to 8.0-8.4, adding ammonium sulfate every 2h to control ammonia nitrogen concentration to 80-100mg/L, controlling temperature of nitrifying sludge liquid to 32 deg.C by electric heating, and performing enrichment culture on nitrifying bacteria in the sludge liquid for 5 days until the number of nitrifying bacteria reaches 5 × 107the sludge concentration in the sludge liquid reaches 5000 mg/mL;
2) and (3) adsorption saturation of a denitrified layer: filling water into a denitrification layer taking zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 100mg/L, namely the zeolite is saturated in the ammonia nitrogen equilibrium concentration;
3) Inoculation: inoculating the sludge liquid treated in the step 1) into the denitrified layer treated in the step 2), adding a nutrient, and controlling the content of dissolved oxygen to be 6.0 mg/L; the nutrient is prepared from ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous sulfate heptahydrate, sodium chloride and magnesium sulfate, and the mass ratio of the nutrient to the sodium chloride is 36: 42: 8: 4: 6: 4; adding ammonium sulfate at intervals to control the ammonia nitrogen concentration to be 50-100mg/L, adding sodium bicarbonate until the pH value is 7.8-8.2, carrying out aerobic culture for 2 days, taking out the zeolite filler to find that thicker mucosa exists on the surface and observing under a microscope to find a large number of microorganisms, namely realizing the rapid biofilm formation of nitrobacteria; FIG. 1 is a photomicrograph of the zeolite surface-coated microorganisms of this example.
4) Water test operation: and continuously feeding the water into an aerated biological filter according to 30%, 60% and 100% of the designed load water amount, monitoring the ammonia nitrogen value of inlet and outlet water, and finishing the start of the BAF microorganisms after the ammonia nitrogen value of the outlet water stably reaches the standard.
The micrograph of the zeolite surface-coated microorganism of example 3 is shown in FIG. 1.
Comparative example:
a microorganism start method of a biological aerated filter is characterized in that microorganisms in the biological aerated filter are upflow biological aerated filters, cobblestones with the height of 0.2 m and the grain diameter of 15-20mm are paved above water inlet and air inlet distribution pipes at the bottom of the biological aerated filter to serve as a bearing layer; the upper part of the bearing layer is filled with 5.0m of ceramsite, the particle size of the ceramsite is 3-5mm, the porosity is 45 percent, and the specific surface area is 5m2/g；
Nitrified sludge of urban domestic sewage plants is taken, the sludge concentration is 2000mg/L, the nitrified sludge is put into a biological aerated filter, aeration is carried out for 3 days, and 2.0-5.0mg/L of dissolved oxygen is maintained. According to the carbon: nitrogen: the mass ratio of phosphorus is 100: 5: 1 adding glucose, urea and potassium dihydrogen phosphate as a nutrient;
Inoculation: and (3) adding the nitrified sludge into ceramsite, wherein the weight ratio of carbon: nitrogen: the mass ratio of phosphorus is 100: 5: 1 adding glucose, urea and potassium dihydrogen phosphate as nutrients to carry out aerobic culture; observing and finding a large amount of microorganism biofilm formation under a microscope; water test operation: and continuously feeding 25%, 50%, 75% and 100% of the designed load water into the biological aerated filter, maintaining for 3 days in each stage, monitoring the ammonia nitrogen values of inlet and outlet water, and finishing the start of the BAF microorganisms after the ammonia nitrogen of outlet water stably reaches the standard.
Performance detection
The sewage treatment conditions of the microorganism start-up methods of the biological aerated filter of examples 1 to 3 and comparative example 1 are shown in the following table:
TABLE 1 Sewage treatment conditions
As can be seen from the table above, the start-up method provided by the application has the advantages that the biofilm formation time is within 1 week, and the enrichment culture of the nitrified sludge and the biofilm formation of the nitrifying bacteria can be separately carried out, so that the biofilm formation start-up time can be shortened to 2-3 days, the stop time of the continuous treatment of the biological filter is greatly shortened, and the ammonia nitrogen treatment capacity is improved. The starting method provided by the inventionThe ammonia nitrogen removal rate after film formation is up to 88-94%, and the treatment load is up to 0.86-0.95 kg NH+ 4-N/(m3d), the ammonia nitrogen removal capability is improved by 2-3 times compared with that of the traditional method of taking ceramsite as the carrier filler of the denitrification layer.
the above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims (10)

1. A microbial start-up method for a biological aerated filter is characterized by comprising the following steps:
1) Sludge treatment: adding a nutrient and trace elements into nitrified sludge of an urban domestic sewage plant, and performing aerobic aeration; the sludge concentration is 1500-2500 mg/L; the microelements comprise manganese, zinc, cobalt, boron, molybdenum, copper and nickel; adjusting the pH value to 8.0-9.0, controlling the ammonia nitrogen concentration to 80-100mg/L and the temperature to 30-35 ℃, and carrying out enrichment culture on nitrifying bacteria in the sludge;
2) Adsorption saturation of zeolite in a denitrification layer: filling water into a denitrification layer taking zeolite as filler, and continuously adding ammonium sulfate to keep the ammonia nitrogen concentration in the water unchanged at 80-120mg/L, namely the zeolite is saturated in the ammonia nitrogen equilibrium concentration;
3) Inoculation: inoculating the sludge treated in the step 1) into the denitrification layer treated in the step 2), adding a nutrient, controlling the ammonia nitrogen concentration to be 50-100mg/L, adjusting the pH to be 7.5-8.5, and carrying out aerobic culture for several days to obtain a biofilm of nitrobacteria;
the nutrient comprises ammonium sulfate, sodium bicarbonate, dipotassium hydrogen phosphate, ferrous salt, sodium salt and magnesium salt, wherein the total proportion of the ammonium sulfate and the sodium bicarbonate in the nutrient is 76-87 wt%.
2. A microbial start-up method for a biological aerated filter according to claim 1, wherein in the nutrient, the ferrous salt is ferrous sulfate, the sodium salt is sodium chloride, and the magnesium salt is magnesium sulfate; ferrous salt accounts for 3-5 wt% of the nutritional agent, and sodium salt accounts for 4-6 wt% of the nutritional agent; magnesium salt accounts for 3-5 wt% of the nutritional agent.
3. A microbial start-up method for a biological aerated filter according to claim 1, wherein in step 1), the trace elements are MnSO4·4H2O、ZnSO4·7H2O、CoCl2·6H2O、H3BO4、Na2MoO4·2H2O、CuSO4·5H2o and NiCl2·6H2O composition; wherein, Mn: the mol ratio of Zn, Co, B, Mo, Cu and Ni is 20-25%, 15-20%, 10-15%, 8-15%, 5-10% and 5-10%.
4. A microbial start-up method for a biological aerated filter according to claim 1, wherein in step 1), the weight ratio of the trace elements to the nutrient is 2-6: 100.
5. A microbial start-up method for a biological aerated filter according to claim 1, wherein in step 1), sodium carbonate or sodium bicarbonate is used to adjust the pH.
6. a microorganism starting method for a biological aerated filter according to claim 1, wherein in step 1), the culture is carried out until the amount of nitrifying bacteria is 106-108one/mL.
7. A method for starting up microorganisms in a biological aerated filter according to claim 1, wherein in step 2), the zeolite is natural zeolite or activated zeolite.
8. A microbial start-up method for a biological aerated filter according to claim 7, wherein in step 2), the natural zeolite has a particle size of 3 to 5mm, a porosity of 50 to 60% and a specific surface area of 50 to 100m2/g；SiO2/Al2O3The ratio is 3.5-4.5, and the cation exchange capacity is 150-200 mmol/g.
9. As claimed inThe microbial start-up method of the biological aerated filter according to claim 7, wherein in the step 2), the activated zeolite has a particle size of 3-5mm, a porosity of 60-75% and a specific surface area of 120-200m2/g；SiO2/Al2O3The ratio is less than 4.5, and the cation exchange capacity is 250-400 mmol/g.
10. A microbial start-up method for a biological aerated filter according to claim 1, wherein in step 3), the dissolved oxygen amount for aerobic culture is 4.0 to 6.0 mg/L.
CN201910677131.6A 2019-07-25 2019-07-25 microbial starting method for biological aerated filter Pending CN110563154A (en)

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Cited By (4)

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CN115057590A (en) * 2022-07-08 2022-09-16 华南理工大学 Method for realizing stable nitrosation of medium-low concentration ammonia nitrogen wastewater based on zeolite biological aerated filter
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Publication number Priority date Publication date Assignee Title
CN111285473A (en) * 2019-12-28 2020-06-16 北京翰祺环境技术有限公司 Method for promoting quick start of denitrification and decarburization system by using high-salt-resistant strain
CN111285473B (en) * 2019-12-28 2022-03-08 北京翰祺环境技术有限公司 Method for promoting quick start of denitrification and decarburization system by using high-salt-resistant strain
CN113307361A (en) * 2020-07-31 2021-08-27 广州庄伟环保科技有限公司 Sewage treatment method for starting contact oxidation system by using microbial agent
CN115057590A (en) * 2022-07-08 2022-09-16 华南理工大学 Method for realizing stable nitrosation of medium-low concentration ammonia nitrogen wastewater based on zeolite biological aerated filter
CN115196746A (en) * 2022-07-08 2022-10-18 华南理工大学 Method for strengthening and stabilizing nitrosation through biological regeneration alkali addition after zeolite adsorption of medium-and-low-concentration ammonia nitrogen wastewater

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