CN110553889A - he dyeing process of automatic dyeing machine - Google Patents
he dyeing process of automatic dyeing machine Download PDFInfo
- Publication number
- CN110553889A CN110553889A CN201910883611.8A CN201910883611A CN110553889A CN 110553889 A CN110553889 A CN 110553889A CN 201910883611 A CN201910883611 A CN 201910883611A CN 110553889 A CN110553889 A CN 110553889A
- Authority
- CN
- China
- Prior art keywords
- slices
- alcohol
- staining
- concentration
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004043 dyeing Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 61
- 238000010186 staining Methods 0.000 claims abstract description 44
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 41
- 210000001519 tissue Anatomy 0.000 claims abstract description 39
- 210000003855 cell nucleus Anatomy 0.000 claims abstract description 33
- 239000008096 xylene Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000004140 cleaning Methods 0.000 claims abstract description 16
- 238000012303 cytoplasmic staining Methods 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 6
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims description 68
- 239000012192 staining solution Substances 0.000 claims description 64
- 235000019441 ethanol Nutrition 0.000 claims description 46
- 238000005406 washing Methods 0.000 claims description 37
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims description 27
- 238000002791 soaking Methods 0.000 claims description 25
- 239000008399 tap water Substances 0.000 claims description 13
- 235000020679 tap water Nutrition 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 2
- 210000000805 cytoplasm Anatomy 0.000 abstract description 16
- 238000004040 coloring Methods 0.000 abstract description 5
- 230000000052 comparative effect Effects 0.000 description 5
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 3
- 238000010828 elution Methods 0.000 description 2
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 238000007447 staining method Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 239000000980 acid dye Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
the invention discloses a He dyeing process of an automatic dyeing machine, which comprises the following steps: (1) dewaxing; (2) eluting xylene; (3) the tissue is put into water; (4) cell nucleus staining; (5) cytoplasmic staining; (6) cleaning with alcohol; (7) the tissue is transparent; (8) and (6) sealing. The process of the invention is adopted to dye the slice tissue, the cell nucleus and the cytoplasm are brightly colored, the coloring is uniform, the stability is good, and the color of the cell nucleus and the cytoplasm is clearly compared, so that the judgment of the test result is more accurate.
Description
Technical Field
the invention relates to the technical field of biological tissue sample treatment, in particular to a He dyeing process of an automatic dyeing machine.
Background
hematoxylin-eosin staining method, HE staining method for short, and one of the staining methods commonly used in paraffin section technology. The hematoxylin staining solution is alkaline, and mainly makes the chromatin in the cell nucleus and the nucleic acid in the cytoplasm bluish; eosin is an acid dye that primarily reddens components in the cytoplasm and extracellular matrix. The HE staining method is the most basic and widely used technical method in histology, embryology, pathology teaching and scientific research.
In the existing He staining process, cell tissues are stained by only one jar of hematoxylin staining solution and one jar of eosin staining solution respectively, and the staining force is insufficient for the non-staining and non-complementary measures.
in addition, the existing method has the problems of different dyeing depths of cell nucleuses, different dyeing depths of cytoplasm and over-deep or over-shallow coloring, the dyeing stability is poor, the dyeing of the cell nucleuses and cytoplasm is not bright enough, the contrast is not bright enough, and the judgment of test operators is influenced, so that the accuracy of final experimental judgment is influenced.
disclosure of Invention
The invention aims to provide a He dyeing process of an automatic dyeing machine.
the technical scheme of the invention is as follows:
The He dyeing process of the automatic dyeing machine comprises the following steps:
(1) Dewaxing: dewaxing the tissue slices in xylene for four times, wherein the dewaxing time is 5-8min each time;
(2) Eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene for three times, wherein each time lasts for 1-3 min;
(3) tissue entering into water: sequentially carrying out a tissue water inlet process by using high-concentration to low-concentration gradient alcohol after eluting xylene, soaking for 1-3min in each concentration of alcohol, and then washing with water, wherein the high-concentration to low-concentration alcohol refers to concentration gradient alcohol with volume concentrations of 95%, 85% and 75%;
(4) and (3) cell nucleus staining: after washing, putting the slices into two vats of hematoxylin staining solution for staining, soaking for 5-8min in each vat, putting the slices into a new vat of hematoxylin staining solution, then putting into an old vat of hematoxylin staining solution, then washing, differentiating, returning blue, washing in sequence, observing whether the staining depth is proper under a microscope, and washing blue for 8-10min by tap water;
(5) Cytoplasmic staining: bluing with tap water, sequentially dyeing the slices in eosin staining solution of two jars, soaking for 20-60 s in each jar, placing the slices in eosin staining solution of a new jar, and placing the slices in eosin staining solution of an old jar;
(6) Alcohol cleaning: staining the slices with eosin staining solution, sequentially cleaning tissues with low-concentration to high-concentration gradient alcohol, and washing with anhydrous ethanol for three times (each for 1-3 min), wherein the low-concentration to high-concentration gradient alcohol refers to 75%, 85%, and 95% concentration gradient alcohol by volume concentration;
(7) and (3) tissue transparency: after the alcohol cleaning is finished, putting the slices into dimethylbenzene or an environment-friendly transparent agent for transparency for four times, and soaking for 1-3min each time;
(8) Sealing: the transparent sections were mounted with neutral gum.
Soaking the differentiated revertant blue in 0.05-1% hydrochloric acid ethanol solution for 1-5 s.
the invention has the beneficial effects that:
1. The staining solution for cell nucleus and cytoplasm adopts a method of two vats, wherein the first vat is new and the second vat is old, the new staining solution can lead the central part of the cell nucleus or the cytoplasm to be colored vividly and have good uniformity, and the old staining solution can lead the part which is not completely colored to be fully colored as supplement, thereby increasing the coloring strength and the dyeing stability.
2. according to the invention, the tissue slice is put into the xylene for dewaxing four times, the xylene is eluted by absolute ethyl alcohol for three times after dewaxing, dewaxing is clean, and the xylene is eluted clean after dewaxing, so that the tissue slice is uniformly colored when dyed by the dyeing liquid subsequently, the dyeing boundary of cell nucleuses and cytoplasms is clear, and the cell nucleuses and cytoplasms are colored brightly, so that the contrast of the cell nucleuses and the cytoplasms is clear, and the test accuracy is high.
Detailed Description
The present invention is further described below.
Example 1
The He dyeing process of the automatic dyeing machine comprises the following steps:
(1) Dewaxing: dewaxing the tissue slices in xylene for four times, wherein the dewaxing time is 5min each time;
(2) eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene for three times, 1min each time;
(3) Tissue entering into water: sequentially carrying out a tissue water inlet process by using alcohol with high concentration to low concentration gradient after eluting xylene, soaking for 1min in the alcohol with each concentration, and then washing with water, wherein the alcohol with high concentration to low concentration refers to the alcohol with concentration gradient of 95%, 85% and 75% in volume concentration;
(4) And (3) cell nucleus staining: after washing, putting the slices into two vats of hematoxylin staining solution for staining, soaking for 5min in each vat, putting the slices into a new vat of hematoxylin staining solution, then putting into an old vat of hematoxylin staining solution, then washing, differentiating, returning blue, washing in sequence, observing whether the staining depth is proper under a microscope, and washing blue for 8min by using tap water;
(5) cytoplasmic staining: after bluing with tap water, successively placing the slices into two vats of eosin staining solution for staining, soaking for 20s in each vat, placing the slices into the eosin staining solution of the newer vat, and then placing the slices into the eosin staining solution of the older vat;
(6) alcohol cleaning: staining the slices with eosin staining solution, sequentially cleaning tissues with low-concentration to high-concentration gradient alcohol, and washing with anhydrous ethanol for three times, each time for 1min, wherein the low-concentration to high-concentration gradient alcohol refers to 75%, 85%, and 95% concentration gradient alcohol by volume concentration;
(7) and (3) tissue transparency: after the alcohol cleaning is finished, putting the slices into dimethylbenzene or an environment-friendly transparent agent for transparency for four times, and soaking for 1min each time;
(8) sealing: the transparent sections were mounted with neutral gum.
soaking the differentiated revertant blue in 0.5-1% hydrochloric acid ethanol solution for 1 s.
example 2
The He dyeing process of the automatic dyeing machine comprises the following steps:
(1) dewaxing: dewaxing the tissue slices in xylene for four times, wherein the dewaxing time is 6min each time;
(2) eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene for three times, 2min each time;
(3) tissue entering into water: sequentially carrying out a tissue water inlet process by using alcohol with high concentration to low concentration gradient after eluting xylene, soaking for 2min in the alcohol with each concentration, and then washing with water, wherein the alcohol with high concentration to low concentration refers to the alcohol with concentration gradient of 95%, 85% and 75% in volume concentration;
(4) and (3) cell nucleus staining: after washing, putting the slices into two vats of hematoxylin staining solution for staining, soaking for 7min in each vat, putting the slices into a new vat of hematoxylin staining solution, then putting into an old vat of hematoxylin staining solution, then washing, differentiating, returning blue, washing in sequence, observing whether the staining depth is proper under a microscope, and washing blue for 9min by using tap water;
(5) Cytoplasmic staining: bluing with tap water, sequentially dyeing the slices in eosin staining solution in two jars, soaking for 40s in each jar, and placing the slices in eosin staining solution in the new jar and then in eosin staining solution in the old jar;
(6) Alcohol cleaning: staining the slices with eosin staining solution, sequentially cleaning tissues with low-concentration to high-concentration gradient alcohol, and washing with anhydrous ethanol for three times (2 min each time), wherein the low-concentration to high-concentration gradient alcohol refers to 75%, 85% and 95% concentration gradient alcohol by volume concentration;
(7) and (3) tissue transparency: after the alcohol cleaning is finished, putting the slices into dimethylbenzene or an environment-friendly transparent agent for transparency for four times, and soaking for 2min each time;
(8) sealing: the transparent sections were mounted with neutral gum.
Soaking the differentiated revertant blue in 0.5-1% hydrochloric acid ethanol solution for 3 s.
example 3
the He dyeing process of the automatic dyeing machine comprises the following steps:
(1) Dewaxing: dewaxing the tissue slices in xylene for four times, wherein the dewaxing time is 8min each time;
(2) Eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene for three times, 3min each time;
(3) Tissue entering into water: sequentially carrying out a tissue water inlet process by using alcohol with high concentration to low concentration gradient after eluting xylene, soaking for 3min in the alcohol with each concentration, and then washing with water, wherein the alcohol with high concentration to low concentration refers to the alcohol with concentration gradient of 95%, 85% and 75% in volume concentration;
(4) And (3) cell nucleus staining: after washing, putting the slices into two vats of hematoxylin staining solution for staining, soaking for 8min in each vat, putting the slices into a new vat of hematoxylin staining solution, then putting into an old vat of hematoxylin staining solution, then washing, differentiating, returning blue, washing in sequence, observing whether the staining depth is proper under a microscope, and washing blue for 10min by using tap water;
(5) Cytoplasmic staining: after bluing with tap water, successively placing the slices into two vats of eosin staining solution for staining, soaking for 60s in each vat, placing the slices into a new vat of eosin staining solution, and then placing the slices into an old vat of eosin staining solution;
(6) alcohol cleaning: staining the slices with eosin staining solution, sequentially cleaning tissues with low-concentration to high-concentration gradient alcohol, and washing with anhydrous ethanol for three times (each for 3 min), wherein the low-concentration to high-concentration gradient alcohol refers to 75%, 85% and 95% concentration gradient alcohol by volume concentration;
(7) And (3) tissue transparency: after the alcohol cleaning is finished, putting the slices into dimethylbenzene or an environment-friendly transparent agent for transparency for four times, and soaking for 3min each time;
(8) sealing: the transparent sections were mounted with neutral gum.
Soaking the differentiated revertant blue in 0.5-1% hydrochloric acid ethanol solution for 5 s.
in the above examples 1-3, the hematoxylin staining solution in the old or new vat is determined relative to the number of times of staining slices in the hematoxylin in the one vat, for example, a 350ml hematoxylin staining solution in one vat can stain 2500-3000 slices, and then the original hematoxylin staining solution is poured off and added with new hematoxylin staining solution, so that the vat into which the hematoxylin staining solution is just added is the new vat, and the vat with the smaller number of times of staining slices is called as the hematoxylin staining solution of the new vat; the definition principle of the newer vat eosin staining solution or the older vat eosin staining solution is the same as that of the hematoxylin staining solution.
After staining the section by hematoxylin staining solution, washing the section by water and differentiating the section by 0.5 to 1 percent of hydrochloric acid ethanol so as to remove the staining of tissues which should not be stained and the transitional staining of the tissues which should be stained, so that the staining depth of the tissues is proper; the bluing was performed by washing with tap water in order to make hematoxylin-stained nuclei blue.
comparative example 1
this example differs from example 1 in that:
Dewaxing in step (1): dewaxing the tissue slices in xylene twice, wherein the dewaxing time is 5min each time;
Step (2) eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene once, 1min each time;
Other contents of this embodiment are the same as embodiment 1.
Comparative example 2
this example differs from example 1 in that:
And (4) cell nucleus staining: after washing, dyeing the slices in a jar of hematoxylin staining solution, soaking for 5min, then sequentially washing, differentiating, turning blue, washing, observing whether the dyeing depth is proper under a microscope, and washing the blues for 8min by using tap water;
step (5) cytoplasmic staining: after bluing with tap water, the slices were stained in a jar of eosin staining solution and soaked for 20 seconds.
other contents of this embodiment are the same as embodiment 1.
The effect of staining the tissue of the sections of examples 1 to 3 and comparative examples 1 and 2 is shown in Table 1 below:
TABLE 1
The number of times of dewaxing was small in comparative example 1 compared to example 1, and the xylene elution number after dewaxing was small, and it is clear from the test results in table 1 that dewaxing and xylene elution are not clean, which results in uneven staining of the slice and unclear boundaries of cell nuclei and cytoplasm; the method adopts xylene for four times of dewaxing, the dewaxing is clean, and the xylene after dewaxing is eluted cleanly, so that the dyeing liquid is used for dyeing in the subsequent process, the dyeing liquid is uniform in coloring, and the dyeing boundaries of cell nucleus and cytoplasm are clear.
comparative example 2 compared to example 1, the cell nucleus was stained with only one jar of hematoxylin staining solution and the cell nucleus was stained with only one jar of eosin staining solution, and it is understood from the test results in table 1 that if the cell nucleus and cell nucleus staining process is not performed with the multi-cylinder immersion staining of new and old forms, the staining intensity is not good, the staining stability is not good, and the cell nucleus and cell nucleus color is not bright enough and the contrast is not sharp enough.
The slice tissues are dyed by two cylinders of hematoxylin staining solutions in a new and an old way, the center of a cell nucleus is stained by the new cylinder of hematoxylin staining solution, the cell nucleus is additionally stained by the old cylinder of hematoxylin staining solution and mostly positioned at the periphery of the cell nucleus, and when washing, differentiation and bluing are carried out subsequently, only the old hematoxylin staining solution at the periphery of the cell nucleus is washed away, and the central tissue of the cell nucleus is kept to be stained by the new cylinder of hematoxylin staining solution, so that the staining stability is good, and the bright and uniform staining of the cell nucleus tissues is ensured; staining cytoplasm of the slice tissue by adopting two vats of eosin staining solution, wherein the staining is the same as the nucleus staining; therefore, the staining solution for cell nucleus and cytoplasm adopts a method of two vats, wherein the first vat is new and the second vat is old, the new staining solution can lead the central part of the cell nucleus or the cytoplasm to be colored vividly and have good uniformity, and the old staining solution can lead the part which is not completely colored to be fully colored as supplement, thereby increasing the coloring strength and the dyeing stability.
Claims (3)
1. The He dyeing process of the automatic dyeing machine is characterized by comprising the following steps of:
(1) dewaxing: dewaxing the tissue slices in xylene for four times, wherein the dewaxing time is 5-8min each time;
(2) Eluting xylene: after dewaxing, putting the slices into absolute ethyl alcohol to elute xylene for three times, wherein each time lasts for 1-3 min;
(3) tissue entering into water: after eluting xylene, sequentially carrying out tissue water inlet process by using alcohol with high concentration to low concentration gradient, soaking in alcohol with each concentration for 1-3min, and then washing with water;
(4) and (3) cell nucleus staining: after washing, putting the slices into two vats of hematoxylin staining solution for staining, soaking for 5-8min in each vat, putting the slices into a new vat of hematoxylin staining solution, then putting into an old vat of hematoxylin staining solution, then washing, differentiating, returning blue, washing in sequence, observing whether the staining depth is proper under a microscope, and washing blue for 8-10min by tap water;
(5) Cytoplasmic staining: bluing with tap water, sequentially dyeing the slices in eosin staining solution of two jars, soaking for 20-60 s in each jar, placing the slices in eosin staining solution of a new jar, and placing the slices in eosin staining solution of an old jar;
(6) Alcohol cleaning: staining the slices with eosin staining solution, sequentially cleaning with low-concentration to high-concentration gradient alcohol, and washing with anhydrous ethanol for three times for 1-3min each time;
(7) and (3) tissue transparency: after the alcohol cleaning is finished, putting the slices into dimethylbenzene or an environment-friendly transparent agent for transparency for four times, and soaking for 1-3min each time;
(8) Sealing: the transparent sections were mounted with neutral gum.
2. the He dyeing process for automatic dyeing machine according to claim 1, characterized in that the high concentration to low concentration alcohol of step (3) is a concentration gradient alcohol with a volume concentration of 95%, 85%, 75%, and the low concentration to high concentration alcohol of step (6) is a concentration gradient alcohol with a volume concentration of 75%, 85%, 95%.
3. The He dyeing process of the automatic dyeing machine according to claim 1, characterized in that the differentiated bluing of step (4) is soaked with 0.5% -1% ethanol solution of hydrochloric acid for 1-5 s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910883611.8A CN110553889A (en) | 2019-09-18 | 2019-09-18 | he dyeing process of automatic dyeing machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910883611.8A CN110553889A (en) | 2019-09-18 | 2019-09-18 | he dyeing process of automatic dyeing machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110553889A true CN110553889A (en) | 2019-12-10 |
Family
ID=68740728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910883611.8A Pending CN110553889A (en) | 2019-09-18 | 2019-09-18 | he dyeing process of automatic dyeing machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110553889A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113324822A (en) * | 2021-06-30 | 2021-08-31 | 艾佧科技(北京)有限公司 | HE staining method for bone tissue abrasive disc of implant material of resin embedding belt |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1422526A1 (en) * | 2002-10-28 | 2004-05-26 | MTM Laboratories AG | Method for improved diagnosis of dysplasias |
| US20070020697A1 (en) * | 2005-07-25 | 2007-01-25 | Hernani Cualing | Virtual flow cytometry on immunostained tissue-tissue cytometer |
| CN101603068A (en) * | 2009-06-23 | 2009-12-16 | 山东省医学科学院基础医学研究所 | The application of Hematorylin in measuring cell-proliferation activity and medicine pair cell toxic effect |
| CN102876081A (en) * | 2012-09-24 | 2013-01-16 | 广州鸿琪光学仪器科技有限公司 | Hematoxylin staining solution and papanicolaou staining kit containing same |
| CN106092707A (en) * | 2016-07-19 | 2016-11-09 | 南昌德漫多科技有限公司 | A kind of apparatus and method for same tissue slice being carried out repeatedly immunostaining |
| CN106644656A (en) * | 2017-01-06 | 2017-05-10 | 杨海玉 | Hematoxylin-eosin one-step dyeing method |
| CN107490511A (en) * | 2017-07-25 | 2017-12-19 | 四川金域医学检验中心有限公司 | A kind of haematoxylin dyeing liquid and HE colouring methods |
| CN108956241A (en) * | 2017-05-26 | 2018-12-07 | 深圳大学 | The multiple staining method of organization chip |
-
2019
- 2019-09-18 CN CN201910883611.8A patent/CN110553889A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1422526A1 (en) * | 2002-10-28 | 2004-05-26 | MTM Laboratories AG | Method for improved diagnosis of dysplasias |
| US20070020697A1 (en) * | 2005-07-25 | 2007-01-25 | Hernani Cualing | Virtual flow cytometry on immunostained tissue-tissue cytometer |
| CN101603068A (en) * | 2009-06-23 | 2009-12-16 | 山东省医学科学院基础医学研究所 | The application of Hematorylin in measuring cell-proliferation activity and medicine pair cell toxic effect |
| CN102876081A (en) * | 2012-09-24 | 2013-01-16 | 广州鸿琪光学仪器科技有限公司 | Hematoxylin staining solution and papanicolaou staining kit containing same |
| CN106092707A (en) * | 2016-07-19 | 2016-11-09 | 南昌德漫多科技有限公司 | A kind of apparatus and method for same tissue slice being carried out repeatedly immunostaining |
| CN106644656A (en) * | 2017-01-06 | 2017-05-10 | 杨海玉 | Hematoxylin-eosin one-step dyeing method |
| CN108956241A (en) * | 2017-05-26 | 2018-12-07 | 深圳大学 | The multiple staining method of organization chip |
| CN107490511A (en) * | 2017-07-25 | 2017-12-19 | 四川金域医学检验中心有限公司 | A kind of haematoxylin dyeing liquid and HE colouring methods |
Non-Patent Citations (2)
| Title |
|---|
| 毛成毅等: "苏木素复染法在HE染色中的应用", 《百度学术》 * |
| 葛运飞: "MACC1、SIRT1、MTA3在EGJA中的表达与肿瘤侵袭相关临床病理因素及预后的关系", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113324822A (en) * | 2021-06-30 | 2021-08-31 | 艾佧科技(北京)有限公司 | HE staining method for bone tissue abrasive disc of implant material of resin embedding belt |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112014192A (en) | HE staining method for paraffin section of northern Guizhou goat ovary tissue | |
| CN107490511B (en) | A kind of haematoxylin dyeing liquid and HE colouring method | |
| CN105651580B (en) | A kind of dyed blended liquid in haematoxylin Yihong | |
| CN107917832A (en) | The production method of fish ovary tissue paraffin section | |
| CN102876081B (en) | Hematoxylin staining solution and papanicolaou staining kit containing same | |
| CN105643745B (en) | The method for improving wood staining dye-uptake | |
| CN103725040A (en) | Hematoxylin eosin staining solution and preparation method thereof | |
| CN106872715A (en) | For the method and composition of hematoxylin eosin stain | |
| CN104389032B (en) | A kind of Degumming method being applicable to containing green fluorescent protein silk | |
| CN106644656A (en) | Hematoxylin-eosin one-step dyeing method | |
| CN110553889A (en) | he dyeing process of automatic dyeing machine | |
| CN107279130A (en) | The double dyeing plastic packaging Slide processings of fish bone | |
| CN110672395A (en) | HE staining method for tissue section | |
| CN110132673B (en) | Method for preparing needle mushroom fruiting body tissue slices | |
| CN104777026A (en) | Liquid-dropping type automatic biological staining instrument | |
| CN105973663A (en) | Production method of colorful bone sample | |
| CN104897453A (en) | Rapid organ tissue dyeing method | |
| CN103926131B (en) | The hexamine Yin Masong resisdye method improved | |
| CN108896376B (en) | Improved HE colouring method | |
| CN101897328A (en) | Method for staining protozoon sample | |
| CN104568551A (en) | Acid resistant staining method | |
| CN107237179A (en) | A kind of cylinder yarn low aberration dyeing | |
| CN109900537A (en) | A kind of processing method of histological tissue specimen | |
| CN110823666A (en) | Application of hematoxylin in preparation of staining agent for reducing color fading of pathological conventional section after staining | |
| CN112067409A (en) | Improved H.E staining method for mouse bone marrow tissue section |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191210 |