CN110522690A - Antioxidant composition containing camellia and the soothing oil containing the antioxidant composition - Google Patents
Antioxidant composition containing camellia and the soothing oil containing the antioxidant composition Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/31—Hydrocarbons
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/63—Steroids; Derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention relates to a kind of camellia antioxidant composition and containing the soothing oil of the antioxidant composition, the antioxidant composition includes each component of following weight ratio: Camellia extract: natural VE: phytosterol: plant saualane is 0.05-1:0.01-1:0.01-1:0.01-1.By Camellia extract, phytosterol, natural VE, plant saualane carries out specific combination, prepares the dedicated antioxidant of grease, ingredient accounting is more scientific, and thermal stability and antioxidant effect are significant.And camellia antioxidant composition of the present invention is added in soothing oil, under the specific combination of each natural, soothing oil shelf life of products and oil oxidative stability are greatly improved.The further addition fermentation camellia product in soothing oil of the present invention, and by being compounded with wheat-germ oil, Sweet Almond Oil, grape seed oil, avocado oil and Pure Olive Oil, the functional characteristic and use feeling of gained soothing oil be greatly improved.
Description
Technical field
The present invention relates to a kind of antioxidant composition containing camellia and containing the soothing oil of the antioxidant composition, it to be used for grease
Class product it is anti-oxidant, it is anti-corruption effect, belong to cosmetic technical field.
Background technique
Successful cosmetics not only provide predetermined function characteristic, but also consumer should be enabled to be satisfied with sensory experience.Makeup
Product must it is odorous it is good, appearance is graceful, feels good, good taste etc..Most each usage quantities of cosmetics are few, but try
Long with the phase, cosmetic formulations Shi Bixu uses qualified raw material, reliable formula and production technology, it is ensured that product is in validity period
Between, persistently keep stable characteristic.
However, cosmetics majority contains all kinds of natural plant greases, natural plant grease is mainly from vegetable seeds and fruit
Real, wherein the ingredient of natural plant grease determines the function and advantage of grease, but also determines stability；High-quality it is natural
Vegetable fat contains highly unsaturated fatty acid, and advantage is more, and stability is poor.Such as unsaturated fatty acid is in long-time storage process
In, hot due to light, the water and enzyme in oxygen and grease in air occur oxidation, lead to cosmetics especially moisturizing
Discoloration or spoiled occurs for the product of oils, will appear the taste of rancidity or other corruption under serious conditions.
The oxidative rancidity for preventing vegetable fat is addition antioxidant, currently, cosmetics antioxidant is main in the market
All it is artificial synthesized antioxidant, has BHT (Butylated Hydroxytoluene), BHA (Butylated Hydroxyanisole), PG (propyl gallate), TBHQ (tert-butyl
Quinhydrones), antioxidant effect is significant, but safety is denounced by consumer always, Waldop etc. once to artificial synthesized BHA, BHT,
TBHQ antioxidant carries out high dose animal testing, studies have shown that BHA, BHT, TBHQ can cause apparent hepatomegaly, BHT is also
The activity of liver and lung microsomal enzyme can be made to increase, so that the other materials of human intake be made to become toxic or carcinogenic substance.Therefore, beautiful
The approval that Food and Drug Administration, state (FDA) has eliminated BHT in 1977 from GRAS (it is widely recognized that safety food) makes
With；Japanese then limit and use in nineteen eighty-two, many Eurasian countries also in succession limit the use of synthetized oxidation preventive agent.
Therefore, a kind of new type natural antioxidant composition is prepared to necessitate.
Natural is mainly derived from the substance of natural safety, such as vegetables, fruit, Chinese herbal medicine, spice and plant
Product etc. of the object through microbial fermentation, it is comparatively safe, it has no toxic side effect, consumer's heart can be eliminated to synthetized oxidation preventive agent
Distrust is significant in terms of the quality and people's health level for improving cosmetics.Anioxidant phytochemicals can be by mentioning
Receptor for hydrogen atom as free radical, the metal ion destroyed in the chain reaction or chelating grease of fatty acid oxidation reach anti-
The effect of oxidation.
But existing natural plant antioxidant is all with existing market hot topic, the significant oxidation resistant plant of anti-oxidation efficacy
Extract carries out scientific combination, external addition；If antioxygenic property preferably day can be used in grease type product development process
Right plant material is product main component, while adding the antioxidant content that oil and fat product itself contains, and is contained by grease itself
Oxidation-resistant active ingredient ratio, backfill be added product in, greatly improve the stability of product.Moisturizing in the present invention simultaneously
The fermentation camellia seed oil and fermentation camellia, leaf extract, product use feeling being added in the preparation of oil get a promotion, to solve oil
The problems such as class product skin sense is greasy, absorption difference, skin-friendly is bad provides very good solution approach.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of antioxidant composition containing camellia and contain this anti-oxidant group
The soothing oil of object is closed, the antioxidant composition containing camellia can be used for the effect of natural oil lipid product increases stability.
The purpose of the present invention is what is be achieved through the following technical solutions:
The present invention provides a kind of camellia antioxidant composition, each component including following weight ratio:
Camellia extract: natural VE: phytosterol: plant saualane is 0.05-1:0.01-1:0.01-1:0.01-1.
Preferably, the natural VE, phytosterol, plant saualane adding proportion be 1.6-1.7:1:1.5-1.6.
Preferably, the Camellia extract be include camellia seed oil, camellia flower extract and camellia leaf extract；The mountain
The mass ratio of tea-seed oil, camellia flower extract and camellia leaf extract is 92-99:0.5-5:0.5-2.5.
The present invention also provides a kind of soothing oil of antioxidant composition containing camellia, each component including following parts by weight:
Preferably, each component including following parts by weight:
Preferably, it is described fermentation camellia product preparation method the following steps are included:
Using camellia seed crude oil and the mixture of camellia floral leaf as fermentation raw material, strain is added and ferments；Institute after fermentation
It obtains product and carries out refinement treatment to get the fermentation camellia product.
Preferably, the strain is Candida sp.SY16；The fermentation condition are as follows: 30 DEG C of temperature, revolving speed 300rpm,
Ventilatory capacity is 1vvm, cultivates 72h.
Preferably, the culture medium that the fermentation uses is prepared using following components: 15g glucose, 1g NH4NO3, 2.55g
K2HPO4, 0.15gNaH2PO4, 0.5gMgSO4·7H2O, 0.1gCaCl2·2H2O, 0.02gMnSO4·H2O, 1g peptone, and
Adjusting PH is 7.8.
Preferably, in the mixture of the camellia floral leaf, the mass ratio of camellia and camellia leaf is 2:1.
Preferably, the mass ratio of the camellia seed crude oil and the mixture of camellia floral leaf is 30:1-60:1.
Compared with prior art, the present invention have it is following the utility model has the advantages that
1: one kind antioxidant composition containing camellia of the present invention, according to original antioxidant activity in cold press camellia oil
Ingredient selects natural, and the accounting according to these active constituents in cold press camellia oil is deployed, and camellia is extracted
Object, phytosterol, natural VE, plant saualane carry out specific combination, prepare the dedicated antioxidant of grease, ingredient accounting more section
It learns, thermal stability and antioxidant effect are significant, are more suitable for the use of the high-grade skin-protection product based on camellia oil.Simultaneously with chemistry
Antioxidant is compared, and safety is higher.And camellia antioxidant composition of the present invention is added in soothing oil, each natural
Under the specific combination of antioxidant, soothing oil shelf life of products and oil oxidative stability are greatly improved.
2: the present invention in soothing oil using camellia as primary raw material, containing fermentation camellia product, and by with wheat-germ oil,
Sweet Almond Oil, grape seed oil, avocado oil and Pure Olive Oil compounding, the functional characteristic and use feeling of gained soothing oil obtain greatly
Improve.And antioxidant composition containing camellia above-mentioned is further added in the soothing oil, it can be common with fermentation camellia product
Effect, so that the antioxidative stabilizer of product further increases.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.Each component raw material involved in following embodiment and comparative example is the commercially available universal product, related experiment
Method is method commonly used in the art.
Embodiment 1:
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, containing such as
The each component of lower parts by weight:
Ferment camellia product: 31；
Wheat-germ oil: 20；
Sweet Almond Oil: 20；
Grape seed oil: 10；
Avocado oil: 10；
Pure Olive Oil: 10；
Camellia antioxidant composition: 0.2.
Wherein each component of the camellia antioxidant composition containing following parts by weight:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=98:1:1): 0.1,
Natural VE: 0.039,
Phytosterol: 0.024,
Plant saualane: 0.037.
Wherein ferment camellia product the preparation method is as follows:
A1, by camellia seed after physics cold press, collect camellia seed crude oil, filtering, camellia seed crude oil must be clarified, for use；
A2, it after drying camellia, camellia leaf, collects, detection, after moisture content detection is qualified, (moisture content≤
10%) it, crushes, crosses 40 meshes, for use.
A3, strain are selected: Candida sp.SY16 (Candida antarctica KCTC 7804).
The preparation of A4, culture medium: 100g camellia seed crude oil, 3.3g camellia, camellia leaf (flower: leaf=2:1), 15g grape
Sugar, 1g NH4NO3,2.55g K2HPO4,0.15gNaH2PO4,0.5gMgSO47H2O, 0.1gCaCl22H2O,
0.02gMnSO4H2O, 1g peptone, adjusting PH is 7.8；
A5, inoculation access the strain liquid of proper ratio, fermentation under aseptic condition.Fermentation condition is 30 DEG C of cultivation temperature, is turned
Fast 300rpm, ventilatory capacity 1vvm cultivate 72h.
A6, grease separation preparation: first plate-frame filtering, after be dehydrated, decolourized, dewaxing treatment, must ferment camellia product.
Embodiment 2:
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, containing such as
The each component of lower parts by weight:
Ferment camellia product: 31；
Wheat-germ oil: 20；
Sweet Almond Oil: 20；
Grape seed oil: 10；
Avocado oil: 10；
Pure Olive Oil: 10；
Camellia antioxidant composition: 0.2.
Wherein camellia antioxidant composition is following component:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=98:1:1): 0.1
Natural VE: 0.06,
Phytosterol: 0.03,
Plant saualane: 0.01.
Wherein ferment camellia product the preparation method is as follows:
A1, by camellia seed after physics cold press, collect camellia seed crude oil, filtering, camellia seed crude oil must be clarified, for use；
A2, it after drying camellia, camellia leaf, collects, detection, after moisture content detection is qualified, (moisture content≤
10%) it, crushes, crosses 40 meshes, for use.
A3, strain are selected: Candida sp.SY16 (Candida antarctica KCTC 7804).
The preparation of A4, culture medium: 100g camellia seed crude oil, 3.33g camellia, camellia leaf (flower: leaf=2:1), 15g grape
Sugar, 1g NH4NO3,2.55g K2HPO4,0.15gNaH2PO4,0.5gMgSO47H2O, 0.1gCaCl22H2O,
0.02gMnSO4H2O, 1g peptone, adjusting PH is 7.8；
A5, inoculation access the strain liquid of proper ratio, fermentation under aseptic condition.Fermentation condition is 30 DEG C of cultivation temperature, is turned
Fast 300rpm, ventilatory capacity 1vvm cultivate 72h.
A6, grease separation preparation: first plate-frame filtering, after be dehydrated, decolourized, dewaxing treatment, must ferment camellia product.
Embodiment 3:
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, containing such as
The each component of lower parts by weight:
Ferment camellia product: 31；
Wheat-germ oil: 15；
Sweet Almond Oil: 20；
Grape seed oil: 8；
Avocado oil: 12；
Pure Olive Oil: 15；
Camellia antioxidant composition: 0.2.
Wherein each component of the camellia antioxidant composition containing following parts by weight:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=98:1:1): 0.1,
Natural VE: 0.039,
Phytosterol: 0.024,
Plant saualane: 0.037.
Wherein ferment camellia product the preparation method is as follows:
A1, by camellia seed after physics cold press, collect camellia seed crude oil, filtering, camellia seed crude oil must be clarified, for use；
A2, it after drying camellia, camellia leaf, collects, detection, after moisture content detection is qualified, (moisture content≤
10%) it, crushes, crosses 40 meshes, for use.
A3, strain are selected: Candida sp.SY16 (Candida antarctica KCTC 7804).
The preparation of A4, culture medium: 100g camellia seed crude oil, 3.33g camellia, camellia leaf (flower: leaf=2:1), 15g grape
Sugar, 1g NH4NO3,2.55g K2HPO4,0.15gNaH2PO4,0.5gMgSO47H2O, 0.1gCaCl22H2O,
0.02gMnSO4H2O, 1g peptone, adjusting PH is 7.8；
A5, inoculation access the strain liquid of proper ratio, fermentation under aseptic condition.Fermentation condition is 30 DEG C of cultivation temperature, is turned
Fast 300rpm, ventilatory capacity 1vvm cultivate 72h.
A6, grease separation preparation: first plate-frame filtering, after be dehydrated, decolourized, dewaxing treatment, must ferment camellia product.
Embodiment 4:
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, with reality
It is essentially identical to apply 1 component of example, the difference is that only:
Wherein ferment camellia product the preparation method is as follows:
A1, by camellia seed after physics cold press, collect camellia seed crude oil, filtering, camellia seed crude oil must be clarified, for use；
A2, it after drying camellia, camellia leaf, collects, detection, after moisture content detection is qualified, (moisture content≤
10%) it, crushes, crosses 40 meshes, for use.
A3, strain are selected: Candida sp.SY16 (Candida antarctica KCTC 7804).
The preparation of A4, culture medium: 100g camellia seed crude oil, 1.67g camellia, camellia leaf (flower: leaf=2:1), 15g grape
Sugar, 1g NH4NO3,2.55g K2HPO4,0.15gNaH2PO4,0.5gMgSO47H2O, 0.1gCaCl22H2O,
0.02gMnSO4H2O, 1g peptone, adjusting PH is 7.8；
A5, inoculation access the strain liquid of proper ratio, fermentation under aseptic condition.Fermentation condition is 30 DEG C of cultivation temperature, is turned
Fast 300rpm, ventilatory capacity 1vvm cultivate 72h.
A6, grease separation preparation: first plate-frame filtering, after be dehydrated, decolourized, dewaxing treatment, must ferment camellia product.
Embodiment 5
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, it is described
The each component and content of soothing oil are substantially the same manner as Example 1, the difference is that only:
The preparation weight ratio of the camellia antioxidant composition are as follows:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=92:5:2): natural VE: plant steroid
Alcohol: plant saualane=1:1:1:1.
Embodiment 6
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, it is described
The each component and content of soothing oil are substantially the same manner as Example 1, the difference is that only:
The preparation weight ratio of the camellia antioxidant composition are as follows:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=92:5:2): natural VE: plant steroid
Alcohol: plant saualane=0.05:0.01:0.01:0.01.
Embodiment 7
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, it is described
Each component and parts by weight are as follows in soothing oil:
Ferment camellia product: 20；
Wheat-germ oil: 30；
Sweet Almond Oil: 30；
Grape seed oil: 5；
Avocado oil: 5；
Pure Olive Oil: 10；
Camellia antioxidant composition: 0.1.
Wherein camellia antioxidant composition is prepared using component same as Example 5 and ratio.Fermentation camellia product system
Preparation Method is same as Example 5.
Embodiment 8
Ferment camellia product: 41；
Wheat-germ oil: 20；
Sweet Almond Oil: 15；
Grape seed oil: 15；
Avocado oil: 15；
Pure Olive Oil: 5；
Camellia antioxidant composition: 0.5
Wherein camellia antioxidant composition is prepared using component same as Example 5 and ratio.Fermentation camellia product system
Preparation Method is same as Example 5.
Comparative example 1-1:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
Point substantially the same manner as Example 1, the difference is that only: in this comparative example, camellia antioxidant composition is following parts by weight
Each component:
Camellia extract: 0.1
Natural VE: 0.1.
Comparative example 1-2:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
Point substantially the same manner as Example 1, the difference is that only: in this comparative example, camellia antioxidant composition is following parts by weight
Each component:
Camellia extract: 0.1
Natural VE: 0.05,
Plant saualane: 0.05
Comparative example 1-3:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
Point substantially the same manner as Example 1, the difference is that only: in this comparative example, camellia antioxidant composition is following parts by weight
Each component:
Camellia extract: 0.1
Natural VE: 0.05,
Phytosterol: 0.05
Comparative example 1-4:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
Point substantially the same manner as Example 1, the difference is that only: in this comparative example, camellia antioxidant composition is one-component camellia
Extract, addition parts by weight are 0.2.
Comparative example 1-5:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
Point substantially the same manner as Example 1, the difference is that only: in this comparative example, camellia antioxidant composition is containing following parts by weight
Each component: natural VE: 0.1 part, phytosterol 0.05, plant saualane 0.05.
Comparative example 1-6:
This comparative example provides a kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition, group
It is point substantially the same manner as Example 1, it the difference is that only: in the camellia antioxidant composition of this comparative example, Camellia extract
Each composition proportion are as follows: camellia seed oil: camellia flower extract is 98:2.
Comparative example 2-1:
A kind of antioxidant composition containing camellia and the soothing oil containing the antioxidant composition are present embodiments provided, containing such as
The each component of lower parts by weight:
Camellia seed oil: 30；
Wheat-germ oil: 20；
Sweet Almond Oil: 20；
Grape seed oil: 10；
Avocado oil: 10；
Pure Olive Oil: 10；
Camellia antioxidant composition: 0.2.
Wherein each component of the camellia antioxidant composition containing following parts by weight:
Camellia extract (camellia seed oil: camellia flower extract: camellia leaf extract=98:1:1): 0.1,
Natural VE: 0.039,
Phytosterol: 0.024,
Plant saualane: 0.037.
Comparative example 2-2:
This comparative example is substantially the same manner as Example 1, the difference is that only: the preparation of the fermentation camellia product of this comparative example
In step, it is only added without camellia leaf, includes 3.33g camellia in step A4.
Comparative example 2-3:
This comparative example is substantially the same manner as Example 1, the difference is that only: the preparation of the fermentation camellia product of this comparative example
Steps are as follows:
A1, by camellia seed after physics cold press, collect camellia seed crude oil, filtering, camellia seed crude oil must be clarified, for use；
A2, it after drying camellia, camellia leaf, collects, detection, after moisture content detection is qualified, (moisture content≤
10%) it, crushes, crosses 40 meshes, for use.
A3, strain are selected: Candida sp.SY16 (Candida antarctica KCTC 7804).
The preparation of A4, culture medium: 100g camellia seed crude oil, 5g camellia, camellia leaf (flower: leaf=2:1), 15g glucose,
1g NH4NO3,2.55g K2HPO4,0.15gNaH2PO4,0.5gMgSO47H2O, 0.1gCaCl22H2O,
0.02gMnSO4H2O, 1g peptone, adjusting PH is 7.8；
A5, inoculation access the strain liquid of proper ratio, fermentation under aseptic condition.Fermentation condition is 30 DEG C of cultivation temperature, is turned
Fast 300rpm, ventilatory capacity 1vvm cultivate 72h.
A6, grease separation preparation: first plate-frame filtering, after be dehydrated, decolourized, dewaxing treatment, obtain fermentation camellia seed oil, send out
Ferment camellia, leaf extract.
Compliance test result:
1. oil oxidative stability is tested:
Soothing oil containing antioxidant composition prepared by the various embodiments described above and comparative example carries out Oxidation of Fat and Oils and stablizes
Property test, test method referring to " measurement (accelerated oxidation test) of GB/T 21121-2007 animal and plant fat oxidation stability ",
Simultaneously in order to more intuitively observe oxidation resistance of the antioxidant composition containing camellia in grease application, common chemistry is closed
At antioxidant BHT, BHA is compared, the additive amount of additive amount and embodiment and the camellia antioxidant composition in comparative example
Unanimously, it selects blank vegetable oil (no antioxidant addition) to compare, is surveyed using 892 type oil oxidative stability testers
Examination.
Test result such as table 1, oxidation induction time are a common counters for evaluating vegetable oil being oxygenized stability, and oxidation lures
Lead that the time is longer, then the oxidation stability of grease is better, and grease is got over not oxidizable.Comparative example is compared with embodiment, is existed significant
Difference finds that the Oxidation of Fat and Oils induction time longest of embodiment 1 illustrates antioxidant oil resistant rouge oxygen used in embodiment 1 simultaneously
The property changed is best.
Table 1
| Number | Induction time |
| Embodiment 1 | 7.01 |
| Embodiment 2 | 6.81 |
| Embodiment 3 | 6.74 |
| Embodiment 4 | 6.69 |
| Embodiment 5 | 6.31 |
| Embodiment 6 | 6.35 |
| Embodiment 7 | 6.27 |
| Embodiment 8 | 6.21 |
| Comparative example 1-1 | 6.39 |
| Comparative example 1-2 | 6.01 |
| Comparative example 1-3 | 6.23 |
| Comparative example 1-4 | 6.41 |
| Comparative example 1-5 | 6.34 |
| Comparative example 1-6 | 3.41 |
| Comparative example 2-1 | 6.92 |
| Comparative example 2-2 | 6.78 |
| Comparative example 2-3 | 6.84 |
| BHT | 5.26 |
| BHA | 4.95 |
| Blank control (no antioxidant) | 3.03 |
3. anti-oxidant testing experiment:
Soothing oil containing antioxidant composition prepared by the various embodiments described above and comparative example, it is clear to carry out DPPH free radical
Except the anti-oxidant test of rate, while in order to more intuitively observe the oxidation resistance of antioxidant composition, select chemical synthesis anti-
Oxidant BHT and BHA are as control.The specific method is as follows:
Experimental procedure: (1) the DPPH solution of the prepare liquid and 2 × 10-4mol/L that take isometric (2mL) mixes (A1)；(2)
The DPPH solution of isometric dehydrated alcohol (determinand solvent) and 2 × 10-4mol/L is taken to mix (A2)；(3) it takes isometric
Dehydrated alcohol and prepare liquid mix (A3)；(4) after reacting 40min, A1, A2, A3 pipe absorbance value are surveyed at 517nm.Clearance rate
Calculation formula are as follows: clearance rate (%)=[1- (A1-A3)/A2] × 100%
Test result is as shown in table 3, and the DPPH clearance rate of extract is higher, and the antioxygenic property of extract is stronger.Test
As a result it can be indicated with IC50, IC50 value refers to sample concentration when DPPH removes half, and the lower inoxidizability of IC50 value is better.As a result
Showing comparative example and embodiment 1, there are significant differences, while the oxidation resistance of camellia antioxidant composition of the invention is aobvious
It writes.
Table 2
| Number | IC50(mg/ml) |
| Embodiment 1 | 3.887 |
| Embodiment 2 | 3.975 |
| Embodiment 3 | 4.001 |
| Embodiment 4 | 4.011 |
| Embodiment 5 | 4.045 |
| Embodiment 6 | 4.033 |
| Embodiment 7 | 4.098 |
| Embodiment 8 | 5.012 |
| Comparative example 1-1 | 6.414 |
| Comparative example 1-2 | 8.169 |
| Comparative example 1-3 | 9.019 |
| Comparative example 1-4 | 5.880 |
| Comparative example 1-5 | 5.532 |
| Comparative example 1-6 | 14.052 |
| BHT | 9.592 |
| BHA | 10.567 |
| Blank control (no antioxidant) | 15.370 |
4. color difference contrast test
Soothing oil containing antioxidant composition prepared by above-described embodiment 1 and 2 and comparative example 1-1 to 1-7 carries out color
Poor contrast test, respectively at 40 DEG C, high/low temperature recycles (- 18 DEG C~48 DEG C, 48h is a circulation), illumination, normal temperature environment condition
The color variation of lower placement different number of days (0 day, 7 days, 28 days) measurement soothing oil, with DE* value quantization means.Color difference comparison is surveyed
Examination, while in order to more intuitively observe the anti-chromatic aberration ability of antioxidant composition, select chemical synthesis antioxidant BHT and
BHA is as control.The specific method is as follows:
Experimental procedure: (1) " measurement standard specimen " is clicked after taking the blank control sample of isometric (3mL) to be packed into instrument, display is surveyed
Data are tried, data are recorded；(2) it is clicked after taking the prepare liquid of isometric (3mL) to be packed into instrument " measurement sample ", display test number
According to record data.
The result shows that comparative example has differences with embodiment 1 under the conditions of different placements, comprehensive discovery embodiment 1 adds
After entering antioxidant, chromatic aberration difference is small, and more stable, i.e., antioxidant effect is preferable.
Table 3
5. security test:
The soothing oil of the antioxidant composition containing camellia of above-mentioned all embodiment and comparative example preparations is subjected to human body peace
Full property patch test, test method is referring to the human skin patch in " 2015 cosmetics safety technical specification ".Skin envelope
The method of closed form patch test are as follows: selecting the age is personnel 30 of 18-60 one full year of life, and area is selected to be no more than 50mm2, depth about
The qualified spot tester material of 1mm, the reparation tightening cosmetic composition for taking 0.020mL above-described embodiment and comparative example preparation are put into spot tester
Small interior, control wells are blank control (not setting any substance), will be added with the low sensitization glue of spot tester for repairing tightening cosmetic composition
Band is pasted on the forearm song side of subject, is gently pressed with palm and is allowed to equably be pasted on skin, continued for 24 hours.Respectively at removal
Containing repair tightening cosmetic composition spot tester after 30min (after impression disappearance after), for 24 hours with 48h press 4 standard sight dermoreaction of table, and
Record observation result.The patch test for repairing tightening cosmetic composition the results are shown in Table 5.
The experimental results showed that the soothing oil of the antioxidant composition containing camellia of all embodiment and comparative example preparations passes through people
Body patch security test.
4 skin closure type patch test dermoreaction grade scale of table
5 human safety test result of table
| Sample number into spectrum | 30min | 24h | 48h | Overall result |
| Embodiment 1 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Embodiment 2 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Embodiment 3 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Embodiment 4 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Embodiment 5 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Embodiment 6 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-1 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-2 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-3 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-4 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-5 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 1-6 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 2-1 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 2-2 | 0 | 0 | 0 | The no positive reaction of 30 people |
| Comparative example 2-3 | 0 | 0 | 0 | The no positive reaction of 30 people |
6. soothing oil sensory evaluation:
Soothing oil containing antioxidant composition prepared by the various embodiments described above and comparative example, carries out the sense organ of soothing oil
Test is evaluated, test method is as follows: organoleptic indicator's test (using 5 points of systems), test environment: 20 ± 2 DEG C；Sample smears mode:
1 drop sample is taken, is looped on the back of the hand smearing with 1 circle/s speed.
The experimental results showed that the camellia product after addition fermentation is conducive to improve oil compared with unfermentable camellia seed oil
The absorbability of rouge and salubrious degree, meanwhile, and the viscous sense in grease use is effectively reduced, it is more salubrious comfortable after smearing.
6 soothing oil sensory evaluation of table
There are many concrete application approach of the present invention, the above is only a preferred embodiment of the present invention.More than it should be pointed out that
Embodiment is merely to illustrate the present invention, and the protection scope being not intended to restrict the invention.For the common skill of the art
For art personnel, without departing from the principle of the present invention, several improvement can also be made, these improvement also should be regarded as this hair
Bright protection scope.
Claims (10)
1. a kind of camellia antioxidant composition, which is characterized in that each component including following weight ratio:
Camellia extract: natural VE: phytosterol: plant saualane is 0.05-1:0.01-1:0.01-1:0.01-1.
2. camellia antioxidant composition according to claim 1, which is characterized in that the natural VE, phytosterol, plant
The adding proportion of saualane is 1.6-1.7:1:1.5-1.6.
3. camellia antioxidant composition according to claim 1, which is characterized in that the Camellia extract be include camellia
Seed oil, camellia flower extract and camellia leaf extract；The quality of the camellia seed oil, camellia flower extract and camellia leaf extract
Than for 92-99:0.5-5:0.25-2.5.
4. a kind of application of camellia antioxidant composition according to claim 1 in skin care item.
5. a kind of soothing oil of antioxidant composition containing camellia, which is characterized in that each component including following parts by weight:
6. the soothing oil of the antioxidant composition according to claim 5 containing camellia, which is characterized in that including following parts by weight
Each component:
7. the soothing oil of the antioxidant composition according to claim 5 or 6 containing camellia, which is characterized in that the fermentation mountain
The preparation method of tea product the following steps are included:
Using camellia seed crude oil and the mixture of camellia floral leaf as fermentation raw material, strain is added and ferments；Gained produces after fermentation
Object carries out refinement treatment to get the fermentation camellia product.
8. the soothing oil of the antioxidant composition according to claim 7 containing camellia, which is characterized in that the strain is
Candida sp.SY16；The fermentation condition are as follows: 30 DEG C of temperature, revolving speed 300rpm, ventilatory capacity 1vvm, cultivate 72h.
9. the soothing oil of the antioxidant composition according to claim 7 or 8 containing camellia, which is characterized in that the fermentation is adopted
Culture medium is prepared using following components: 15g glucose, 1g NH4NO3, 2.55g K2HPO4, 0.15gNaH2PO4,
0.5gMgSO4·7H2O, 0.1gCaCl2·2H2O, 0.02gMnSO4·H2O, 1g peptone, and adjusting PH is 7.8.
10. the soothing oil of the antioxidant composition according to claim 7 containing camellia, which is characterized in that the camellia floral leaf
Mixture in, the mass ratio of camellia and camellia leaf is 2:1；
The mass ratio of the camellia seed crude oil and the mixture of camellia floral leaf is 30:1-60:1.
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