CN110521564B

CN110521564B - Mycorrhizal seedling raising method for living hypha net infected plant

Info

Publication number: CN110521564B
Application number: CN201910874609.4A
Authority: CN
Inventors: 胡文涛; 唐明; 陈辉; 梁京威
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2019-09-17
Filing date: 2019-09-17
Publication date: 2021-07-20
Anticipated expiration: 2039-09-17
Also published as: CN110521564A

Abstract

The invention discloses a mycorrhizal seedling raising method for a living bacterial silk screen infected plant, which comprises the steps of penetrating a sterile seedling root system of the plant from the outside of a culture container into an inverted planting mode, and inoculating a mycorrhizal fungi agent around the root system; the upper layer frame of the isolation net is tightly attached to the inner wall of the culture container; filling an upper matrix on the isolation net; irrigating the nutrient solution and discharging excessive water through the drain hole; after inoculation by the microbial inoculum and cultivation for six weeks, the plant root system can establish symbiotic relationship with mycorrhizal fungi in the microbial inoculum, after the seeds sowed on the upper layer germinate, the plant planted with the inverted bottom is shaded, and after three weeks, the upper layer seedlings can be successfully mycorrhized. The invention is suitable for various mycorrhizal fungi, can carry out mycorrhizal seedling raising on the upper layer of a culture container for multiple times, has short period and simple operation, does not need to culture a mycorrhizal fungi microbial inoculum and inoculate for multiple times, plants and inoculates on the bottom layer in one-time inversion, can carry out mycorrhizal seedling raising for multiple times throughout the year, and has simple use, time and labor saving and high feasibility.

Description

Mycorrhizal seedling raising method for living hypha net infected plant

Technical Field

The invention relates to a mycorrhizal seedling raising method, in particular to a mycorrhizal seedling raising method for a living mycelium net infected plant, and belongs to the field of microorganisms.

Background

Mycorrhizal fungi are fungi widely symbiotic with plants in soil, are various in variety and can be simply summarized into ectotrophic mycorrhizal fungi, arbuscular mycorrhizal fungi and orchidaceae mycorrhizal fungi, and because the mycorrhizal fungi can establish a mutual-benefit symbiotic relationship with the plants, people widely put the mycorrhizal fungi into practical production and application and research the symbiotic mechanism of the mycorrhizal fungi. No matter the actual production application of the mycorrhizal fungi or the research on symbiotic mechanism, the mycorrhizal fungi and the plants are inevitably required to establish symbiotic relationship, namely, the plant root systems are mycorrhized.

The traditional mycorrhization method is that a mycorrhizal fungi agent is prepared firstly, and then the agent is inoculated around the root system of a plant, so that the mycorrhizal fungi in the agent and the plant establish a symbiotic relationship. The mycorrhizal fungi are different in type, arbuscular mycorrhizal fungi generally adopt spores as a fungicide for inoculation, ectomycorrhizal fungi and orchid mycorrhizal fungi generally adopt solid fungus cakes or liquid mycelial balls of the fungi as the fungicide for inoculation, and the three mycorrhizal fungi fungicides are different in preparation method. At present, a simple and practical mycorrhizal seedling culture method which is easy for mycorrhizal seedling culture, does not need to prepare a mycorrhizal fungi microbial inoculum for many times and is suitable for various mycorrhizal fungi does not exist.

Disclosure of Invention

The invention aims to overcome the defects of the conventional mycorrhizal seedling method and discloses a mycorrhizal seedling method for infecting plants by using a living mycelium mesh.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a. firstly, penetrating the root system of aseptic seedlings of perennial plants from the outside of a plant planting hole of a culture container for inverted planting, inoculating a mycorrhizal fungi agent around the root system, filling one third to one half of the culture container with a lower-layer matrix with the particle size of less than 2mm, and then filling a middle-layer matrix with the thickness of 1-2 cm and the particle size of 3-5 mm;

b. placing an upper layer frame with an isolation net with the aperture of 40-50 mu m on the middle layer substrate, and tightly attaching the periphery of the upper layer frame to the inner wall of the culture container so that the root system of the lower layer cannot pass through the attachment part of the upper layer frame and the culture container;

c. filling the substrate with the grain diameter smaller than 2mm as an upper substrate on the isolation net;

d. placing the culture container at a temperature range of 25-30 ℃ and an illumination intensity range of 5000-10000 Lux;

e. irrigating the low-phosphorus nutrient solution from the upper layer of the culture container once every seven days, wherein distilled water is poured once every 24 hours to keep the humidity in the culture container, and simultaneously discharging excessive water through a water discharge hole;

f. after the mycorrhizal fungi agent is adopted for inoculation and culture for six weeks, the symbiotic relationship between the plant root system and the mycorrhizal fungi in the agent can be established, and then the seedling seeds to be mycorrhized, which are subjected to surface disinfection, are broadcast in the upper-layer matrix;

g. after the seeds germinate, shading the plants planted in the inverted manner at the bottom for three weeks to enable the plants planted in the inverted manner at the bottom to receive illumination of 1000-2000 Lux, and after three weeks, the upper seedlings can be mycorrhized successfully, digging out the mycorrhized seedlings for practical application or scientific research, then filling a new upper substrate, and removing shading from the plants planted in the inverted manner at the bottom for two weeks before carrying out mycorrhization seedling culture of the next round.

The method adopts the inverted planting of the root system of the perennial plant and the mycorrhizal fungi to establish the symbiotic relationship at the lower layer of the culture container so as to maintain the growth of the mycorrhizal fungi, can ensure that a hypha net of the mycorrhizal fungi is spread over the whole culture container and is suitable for various mycorrhizal fungi, after the hypha net of the mycorrhizal fungi is established in the culture container, the mycorrhizal fungi seedling culture can be carried out on the upper layer of the culture container for a plurality of times, the period is short, the operation is simple, the preparation of the mycorrhizal fungi agent and the inoculation for a plurality of times are not needed, the bottom layer plant is planted and inoculated in one inverted planting manner, the mycorrhizal seedling culture can be carried out for a plurality of times throughout the year, the use is simple, the time and the labor are saved, and the feasibility is high.

Drawings

FIG. 1 is a schematic sectional view of a front view structure of the present invention.

The reference numbers of figure 1 are as follows: the cultivation container 1, the plant planting hole 2, the drain hole 3, the upper layer frame 4, the isolation net 5, the lower layer substrate 6, the middle layer substrate 7 and the upper layer substrate 8.

Detailed Description

The present invention will be described in further detail with reference to the following specific embodiments and the attached drawings, wherein the embodiments are only for the purpose of illustrating the invention and are not to be construed as limiting the invention:

a. firstly, penetrating the aseptic seedling root system of vetiver grass from the outside of a plant planting hole (2) of a culture container for inverted planting, inoculating a bursa of mockstrawberries (Fusneliformis mossea) microbial inoculum around the root system, filling one third of the culture container (1) with sterilized sand and sterilized vermiculite with the grain diameter of less than 2mm as a lower layer matrix (6), and then filling 1cm of aseptic glass beads with the grain diameter of 4mm as a middle layer matrix (7);

b. placing an upper layer frame (4) with an isolation net (5) with the aperture of 45 mu m on a middle layer substrate (7), and tightly attaching the periphery of the upper layer frame to the inner wall of the culture container so that the root system of the lower layer cannot pass through the attachment part of the upper layer frame (4) and the culture container (1);

c. filling the sterilized sand with the grain diameter less than 2mm and the sterilized vermiculite as an upper substrate (8) on the isolation net;

d. hanging the culture container (1) at a temperature range of 25-28 ℃ and an illumination intensity range of 8000-10000 Lux;

e. pouring a Hoagland nutrient solution with the phosphorus concentration of 1/100 from the upper layer once every seven days, wherein distilled water is poured once every 24 hours to keep the humidity in the culture container, and simultaneously, excessive water is drained through a drain hole (3);

f. after the Moxidou Tubuergerian mycorrhizal fungi agent is adopted for inoculation and culture for six weeks, the vetiver root system can establish symbiotic relationship with the Moxidou Tubuergerian fungi, then tomato seeds to be mycorrhized are broadcast in an upper-layer matrix (8), and the tomato seeds need to be disinfected by 5% of sodium hypochlorite for 5 minutes and washed by sterile water for more than 3 times before broadcast;

g. after the tomato seeds germinate, shading the vetiver grass planted at the bottom in an inverted mode for three weeks to enable the vetiver grass planted at the bottom in the inverted mode to be capable of receiving illumination of 1500-2000 Lux, enabling the upper layer tomato seedlings to be successfully mycorrhized after three weeks, digging out the mycorrhized tomato seedlings for practical application or scientific research, filling upper layer matrix (8) of new sterilized sand and sterilized vermiculite, removing shading from the vetiver grass planted at the bottom for two weeks, and then carrying out mycorrhization seedling raising of the next round.

Claims

1. A mycorrhizal seedling raising method for a living hypha net infected plant is characterized by comprising the following steps: the technical scheme for solving the technical problem is as follows:

a. firstly, the root system of aseptic seedlings of perennial plants penetrates through a plant planting hole (2) of a culture container to be planted in an inverted manner, a mycorrhizal fungi agent is inoculated around the root system, a lower-layer substrate (6) with the particle size of less than 2mm is filled to one third to one half of the culture container (1), and then a middle-layer substrate (7) with the thickness of 1-2 cm and the particle size of 3-5 mm is filled;

b. placing an upper layer frame (4) with an isolation net (5) with the aperture of 40-50 mu m on the middle layer substrate, and tightly attaching the periphery of the upper layer frame to the inner wall of the culture container so that the root system of the lower layer cannot pass through the attachment part of the upper layer frame (4) and the culture container (1);

c. filling a substrate with the particle size of less than 2mm as an upper substrate (8) on the isolation net;

d. placing the culture container (1) at a temperature range of 25-30 ℃ and an illumination intensity range of 5000-10000 Lux;

e. pouring the low-phosphorus nutrient solution from the upper layer of the culture container once every seven days, wherein distilled water is poured once every 24 hours to keep the humidity in the culture container, and simultaneously discharging excessive water through a water discharge hole (3);

f. after the mycorrhizal fungi agent is adopted for inoculation and culture for six weeks, the symbiotic relationship between the plant root system and the mycorrhizal fungi in the agent can be established, and then the seedling seeds to be mycorrhized, which are subjected to surface disinfection, are broadcast in the upper layer matrix (8);

g. after the seeds germinate, shading the plants planted in the inverted manner at the bottom for three weeks to enable the plants planted in the inverted manner at the bottom to receive illumination of 1000-2000 Lux, and after three weeks, the upper seedlings can be mycorrhized successfully, digging out the mycorrhized seedlings for practical application or scientific research, then filling a new upper substrate (8), removing shading from the plants planted in the inverted manner at the bottom for two weeks, and then carrying out mycorrhization seedling culture of the next round.

2. The mycorrhizal seedling raising method for living fungus silk net infected plants according to claim 1, which is characterized in that:

a. firstly, the aseptic seedling root system of vetiver grass is penetrated and inversely planted from the outside of a plant planting hole (2) of a culture container, and a Moxidou Tubuchu fungus agent is planted around the root system, and sterilized sand and sterilized vermiculite with the grain diameter of less than 2mm are used as a lower layer substrate (6) to be filled to one third of the culture container (1), and then 1cm thick aseptic glass beads with the grain diameter of 4mm are filled as a middle layer substrate (7);

f. after the Moxidou Tubuergerian mycorrhizal fungi agent is adopted for inoculation and culture for six weeks, the root system of the vetiver grass begins to establish a symbiotic relationship with the Moxidou Tubuergerian fungus, then tomato seeds to be mycorrhized are broadcast in an upper-layer matrix (8), and the tomato seeds need to be disinfected by 5% of sodium hypochlorite for 5 minutes and washed by sterile water for more than 3 times before broadcast;