CN110508613A - A kind of restorative procedure of polycyclic aromatic hydrocarbon pollution - Google Patents
A kind of restorative procedure of polycyclic aromatic hydrocarbon pollution Download PDFInfo
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Abstract
The present invention provides a kind of restorative procedures of polycyclic aromatic hydrocarbon pollution, belong to technical field of soil remediation.Polycyclic aromatic hydrocarbon pollution is made into Soil slurry using bioactive water by the present invention, make polycyclic aromatic hydrocarbon in polycyclic aromatic hydrocarbon pollution that there is lower distribution coefficient in the Soil slurry sample, specifically, the bioactive water has stronger desorption ability to polycyclic aromatic hydrocarbon, the amount of solute in water phase can be increased, therefore, more polycyclic aromatic hydrocarbons can be dissolved in bioactive water, be convenient for subsequent bio degradation；Moreover, the bioactive water is nontoxic, unlike organic solvent or surfactant etc. may cause secondary pollution；In addition, the bioactive water can activate bacteria metabolism, enhance the utilizability of microorganism, Soil slurry is carried out biodegrade using polycyclic aromatic hydrocarbon-degrading bacteria on the basis of bioactive water, is conducive to bioremediation efficiency by the present invention.
Description
Technical field
The present invention relates to technical field of soil remediation more particularly to a kind of restorative procedures of polycyclic aromatic hydrocarbon pollution.
Background technique
With the development of society, the quickening of process of industrialization, the type and quantity of Industrial " three Waste " increasingly increase severely, into soil
Organic pollutant in earth also increases therewith.Polycyclic aromatic hydrocarbon (Polycyclic Aromatic Hydrocarbons, PAHs) is
Generally existing a kind of persistence organic pollutant (Persistent in soil (including saline-alkali soil and general soil)
Organic Pollutants, POPs), there is stronger teratogenesis, carcinogenic, mutagenesis to human body and other biologies.Therefore,
How effectively to repair PAHs contaminated soil and have become home and abroad environment protection field in recent years and pays close attention to jointly and urgently to be resolved
A great problem.
By 30 years of researches and application, currently form with peripheral doses, chemical-biological joint is repaired, biology is repaired
PAHs contaminated soil remediation technical system based on multiple three kinds of recovery techniques.The biological prosthetic of early stage is primarily referred to as microorganism and repairs
It is multiple, and a kind of earliest, most deep, current most widely used biological renovation method of research.It is polluted using microorganism remediation
Environmental system it is more effective and economical, become an important clean and environmental protection technology.Microorganism is biological prosthetic master
Body occupies an important position during the Transport And Transformation of pollutant or even final removing.Microorganism remediation is substantially exploitation
Using the metabolic capabilities of microorganism and the diversity of gene, pollutant is converted into free of contamination final product, it can
Reenter the biochemical cycles of the earth.
Currently, repairing using microorganism remediation technology to PAHs contaminated soil, it is considered as bioavailability
(bioavailability), wherein microorganism includes: first using organic compound several mechanism that may be present, microorganism
Metabolism is carried out using the organic compound being dissolved in water phase；Second, microorganism, which directly contacts to absorb in soil particle, to be had
The consumption of machine compound progress matrix；Third, the extracellular polymerics such as microorganism secretion ectoenzyme or EPS carry out biodegrade or inhale
Biodegrade is carried out after attached pollutant.In order to improve or the efficiency of enhancement microbiological recovery technique, it usually needs addition surface is living
Property agent etc. strengthen the desorption of organic pollutant, increase it is water-soluble, to improve bioavailability etc..But it is easy to draw in this way
The problem of playing secondary pollution, and biological prosthetic efficiency is to be improved.
Summary of the invention
The purpose of the present invention is to provide a kind of restorative procedure of polycyclic aromatic hydrocarbon pollution, method provided by the invention is green
Colour circle is protected, and repairing effect is good.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of restorative procedures of polycyclic aromatic hydrocarbon pollution, comprising the following steps:
Polycyclic aromatic hydrocarbon pollution and bioactive water are mixed, Soil slurry is obtained；
Biodegrade is carried out after the Soil slurry is inoculated with polycyclic aromatic hydrocarbon-degrading bacteria.
Preferably, the raw material for preparing the Soil slurry further includes nutrient matrix.
Preferably, salt content≤15% in the polycyclic aromatic hydrocarbon pollution.
Preferably, the soil type of the polycyclic aromatic hydrocarbon pollution includes loess, black earth or sandy soil.
Preferably, salt content≤12% in the Soil slurry.
Preferably, salt content≤6% in the Soil slurry.
Preferably, the inoculum concentration of the polycyclic aromatic hydrocarbon-degrading bacteria is 1~10%.
Preferably, the biodegradable temperature is 15~45 DEG C, and the time is 1~15d.
The present invention provides a kind of restorative procedures of polycyclic aromatic hydrocarbon pollution, comprising the following steps: by polycyclic aromatic hydrocarbon dirt
Soil and bioactive water mixing are contaminated, Soil slurry is obtained；It is given birth to after the Soil slurry is inoculated with polycyclic aromatic hydrocarbon-degrading bacteria
Object degradation.Polycyclic aromatic hydrocarbon pollution is made into Soil slurry using bioactive water by the present invention, can be made polycyclic aromatic hydrocarbons contaminated
PAHs in soil has lower distribution coefficient in the Soil slurry sample, specifically, the bioactive water is to more
Cycloaromatics has stronger desorption ability, can increase the amount of solute in water phase, therefore, more polycyclic aromatic hydrocarbons can be dissolved in biology
In active water, it is convenient for subsequent bio degradation；Moreover, the bioactive water is nontoxic, unlike organic solvent or surface-active
Agent etc. may cause secondary pollution；In addition, the bioactive water can activate bacteria metabolism, enhance microorganism can
Soil slurry is carried out biodegrade using polycyclic aromatic hydrocarbon-degrading bacteria on the basis of bioactive water by usability, the present invention, favorably
In bioremediation efficiency.
Detailed description of the invention
Fig. 1 is solubility comparison diagram of the NaCl in bioactive water and distilled water；
Fig. 2 is distribution coefficient comparison diagram in fluoranthene gradient contaminated soil mud sample；
Fig. 3 is the biological prosthetic effect picture of 6 kinds of PAHs contaminated soils；
Fig. 4 is growing state figure of degradation bacteria YN1 under the conditions of different salt contents.
Biological deposits explanation:
The classification naming of degradation bacteria YN1: secondary bacillus mycoides；Latin name: Bacillus paramycoides；
Preservation date: on 06 10th, 2019；Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center
(CGMCC), Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, biological study institute, the Chinese Academy of Sciences；Deposit number: CGMCCNo.:
17911。
Specific embodiment
The present invention provides a kind of restorative procedures of polycyclic aromatic hydrocarbon pollution, comprising the following steps:
Polycyclic aromatic hydrocarbon pollution and bioactive water are mixed, Soil slurry is obtained；
Biodegrade is carried out after the Soil slurry is inoculated with polycyclic aromatic hydrocarbon-degrading bacteria.
The present invention mixes polycyclic aromatic hydrocarbon pollution and bioactive water, obtains Soil slurry.In the present invention, described
Polycyclic aromatic hydrocarbon pollution specifically refer to it is any need repair by polycyclic aromatic hydrocarbons contaminated soil；The present invention is for described polycyclic
Salt content does not have special restriction, saline-alkali soil (salt content >=0.7%) either general soil (salt content in aromatic hydrocarbons contaminated soil
< 0.7%), specifically, in the polycyclic aromatic hydrocarbon pollution salt content preferably≤15%.The present invention is for described polycyclic
The soil type of aromatic hydrocarbons contaminated soil does not have special restriction, the soil of any soil property, specifically, can be loess, black
Soil or sandy soil.The present invention does not have special restriction for the type of polycyclic aromatic hydrocarbon in the polycyclic aromatic hydrocarbon pollution, such as includes
Naphthalene, fluorenes, phenanthrene, anthracene, fluoranthene, pyrene etc..Polycyclic aromatic hydrocarbon pollution is preferably crossed the pharmacopeia that aperture is 2mm and sieved by the present invention, takes under sieve
Part uses.
In the present invention, bioactive water (the bioactive water；BW preparation method) preferably includes following step
It is rapid:
Turfy soil, bentonite, calcium alginate, cement and water are mixed, gained mixed slurry is subjected to curing molding, is obtained
Form mixture；
The molding mixture is placed in water with float stone and is aerated, bioactive water is obtained.
The present invention preferably mixes turfy soil, bentonite, calcium alginate, cement and water, and gained mixed slurry is consolidated
Chemical conversion type obtains molding mixture.Type and source of the present invention for the turfy soil, bentonite, calcium alginate and cement
There is no special restriction, using commercial goods well known to those skilled in the art；Wherein, the pH value of the turfy soil is preferred
It is 5.47.
In the present invention, the mass ratio of the turfy soil, bentonite, calcium alginate and cement is preferably (30~50): (10
~30): (0.2~1): (10~20), more preferably (35~45): (15~25): (0.5~0.8): (12~17), further
Preferably 40:20:0.6:15.The amount of water used does not have special restriction when the present invention forms mixture for preparation, can will
Turfy soil, bentonite, calcium alginate and cement mix and stir together, form soft or hard suitable slurry and are convenient for further cured molding
Molding mixture is prepared.
In an embodiment of the present invention, it specifically by turfy soil, bentonite, calcium alginate and cement mixing, is then stirring
Suitable quantity of water is sprayed under the conditions of mixing, and forms soft or hard suitable mixed slurry, the mixed slurry is added in mold and is solidified
Molding obtains molding mixture.The present invention does not have the revolving speed of the stirring special restriction, can mix each material equal
It is even.The present invention does not have special restriction, preferably long 10cm, diameter for the shape and size of the molding mixture
2.5cm's is cylindric.The present invention does not have the operating parameter of the curing molding special restriction, can guarantee cement solidification
Turfy soil, bentonite and calcium alginate are bonded together afterwards, obtain molding mixture.
After obtaining molding mixture, the molding mixture is placed in water with float stone and is aerated by the present invention, is given birth to
Object active water.In the present invention, the mass ratio of the molding mixture and float stone is preferably 1:(1.5~2.5), more preferably 1:
2.In the present invention, the float stone is preferably dimensioned to be 2~10cm.When the present invention is aerated, the molding mixture and floating
The gross mass of stone and the volume ratio of water are preferably (30~40) g:1000mL, more preferably 36g:1000mL.The present invention is preferably sharp
It is aerated with air pump, the flow of air is preferably greater than 0.5L/min, more preferably 0.55~0.60L/min；Aeration when
Between preferably 4~7d, more preferably 4~5d.
After completing the aeration, the present invention will preferably form mixture and float stone filtering removal in system, and gained water is
Bioactive water.
After obtaining bioactive water, the present invention mixes polycyclic aromatic hydrocarbon pollution and bioactive water, obtains soil mud
Slurry.In the present invention, in the Soil slurry salt content preferably≤12%, more preferably≤6%, further preferably 2~6%,
It is still more preferably 4%.Specifically, the present invention is preferably by institute when the salt content of the polycyclic aromatic hydrocarbon pollution≤8%
Polycyclic aromatic hydrocarbon pollution and bioactive water are stated in 1g:(0.5~2) mL ratio mixing (NaCl or others can be properly added
Salt makes salt content 2~4% in Soil slurry), obtain Soil slurry；When the polycyclic aromatic hydrocarbon pollution salt content >
When 8%, it is 8~12% that the polycyclic aromatic hydrocarbon pollution is preferably diluted with water to salinity by the present invention, then that gained is dilute
Soil and bioactive water are released in 1g:(0.5~2) mixing of mL ratio, obtain Soil slurry, wherein the present invention is more for diluting
The water that cyclophane soil contaminated with hydrocarbons uses does not have special restriction, such as practical raw using water well known to those skilled in the art
Underground water can be used in production.
In the present invention, preparing the raw material of the Soil slurry, it is also preferable to include nutrient matrixes, specifically by polycyclic aromatic hydrocarbon
Contaminated soil, bioactive water and nutrient matrix mixing, obtain Soil slurry；The present invention for the nutrient matrix type with
And dosage does not have special restriction, using general inorganic salt culture medium；The present invention provides microorganism life using nutrient matrix
Long required condition.
In the present invention, the temperature of each material mixing is preferably 15~45 DEG C when preparing the Soil slurry, and the time is preferred
For 24~48h.The present invention preferably mixes each component under the conditions of roller bottle, gained mixed material after specifically mixing each component
It is placed on desk-top roller bottle apparatus and carries out Balance Treatment；During Balance Treatment, each component is sufficiently mixed, and in bioactivity
Under the action of water, part polycyclic aromatic hydrocarbon is convenient for subsequent bio degradation by desorption into water phase in polycyclic aromatic hydrocarbon pollution.
After obtaining Soil slurry, the present invention carries out biodegrade after the Soil slurry is inoculated with polycyclic aromatic hydrocarbon-degrading bacteria.
The present invention does not have special restriction for the specific type of the polycyclic aromatic hydrocarbon-degrading bacteria, can be realized the degradation of polycyclic aromatic hydrocarbon i.e.
It can.In the present invention, the inoculum concentration of the polycyclic aromatic hydrocarbon-degrading bacteria is preferably 0.5~10% (v/v).In the embodiment of the present invention
In, in order to verify the validity of the method provided by the present invention, specifically using degradation bacteria YN1 prepared by polycyclic aromatic hydrocarbon pollution
Soil slurry carries out biodegrade；The deposit number of the degradation bacteria YN1 is CGMCC No.:17911.
In the present invention, the biodegradable temperature is preferably 15~45 DEG C；In an embodiment of the present invention, specifically
The biodegrade is carried out at room temperature；The biodegradable time is preferably 1~15d, more preferably 2~10d.This
Invention preferably carries out biodegrade under the conditions of roller bottle, after Soil slurry is specifically inoculated with polycyclic aromatic hydrocarbon-degrading bacteria, is placed in
Biodegrade is carried out on desk-top roller bottle apparatus；In biodegradation process, polycyclic aromatic hydrocarbon is degraded by polycyclic aromatic hydrocarbon-degrading bacteria in system,
And since bioactive water has stronger desorption ability to polycyclic aromatic hydrocarbon, the amount of solute in water phase can be increased, therefore, more
Polycyclic aromatic hydrocarbon is dissolved in bioactive water, is carried out biodegrade convenient for polycyclic aromatic hydrocarbon-degrading bacteria, is conducive to bioremediation
Efficiency.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
1, bioactive water is prepared
Turfy soil (pH value 5.47) 40g, bentonite 20g, calcium alginate 0.6g and cement 15g are mixed, then stirred
Under the conditions of spray appropriate distilled water, form soft or hard suitable mixed slurry, the mixed slurry be added in mold and is consolidated
Chemical conversion type obtains molding mixture；Molding mixture and 24g float stone described in 12g are put into 1000mL distilled water, air is utilized
Pump carries out aeration 4d under conditions of flow is 0.55L/min, and the molding mixture of filtering removal later and float stone obtain biology
Active water (BW).
2, NaCl solubility is analyzed
100mL bioactive water is taken, is filtered with GF/C filter paper (Whatman 1822-047), then distinguishes gained filtrate
It takes 25mL in 3 100mL beakers, places the beaker to heat on magnetic stirring apparatus and stir, bioactivity coolant-temperature gage is maintained at
30 DEG C, it is slowly added to NaCl solid, until NaCl solid cannot be redissolved, beaker is put in cooling in 4 DEG C of refrigerators, every 5min
Observation is primary, and if there is NaCl crystal is precipitated, then solution is saturation state at this time, and saturated solution is stood under the conditions of 30 DEG C
10min.5mL supernatant is drawn from three beakers respectively in 3 known quality (m1, in the weighing bottle of unit g), it is placed in
Solvent evaporated in 100 DEG C of insulating boxs is subsequently placed in drier dry 45min, weighs weighing bottle quality (m later2, unit is
G), solubility of the NaCl in bioactive water under the conditions of 30 DEG C being calculated by Formulas I:
S=(m2-m1) ÷ V × n Formulas I；
In Formulas I, S is solubility, unit g/100mL；
m1For the quality of weighing bottle, unit g；
m2For the gross mass of NaCl and weighing bottle, unit g；
V is the volume of supernatant, specially 5mL；
N be conversion the coefficient of dilution, specially 20.
Solubility of the NaCl in distilled water (DW) under the conditions of measuring 30 DEG C according to the method described above.
Fig. 1 is solubility comparison diagram (in figure error line be standard deviation) of the NaCl in bioactive water and distilled water,
As shown in Figure 1, bioactive water is 34g/100mLBW to the solubility of NaCl, and distilled water is 32g/ to the solubility of NaCl
100mL DW, bioactive water are higher than distilled water to the solubility of NaCl, illustrate that bioactive water increases the solubility of NaCl,
And then it is more advantageous to utilization of the microorganism to NaCl.
3, it analyzes the pollutant cleaning function of bioactive water and measures distribution coefficient
The ratio between compound concentration in the compound concentration and water of absorption in the soil, as distribution coefficient, are indicated with Kd,
Its expression formula is as shown in Formula II:
Kd=Cs/Cc Formula II；
In Formula II, Cs, Cc are respectively equilibrium concentration of the organic compound in soil and water.
5g loess, black earth and sandy soil are weighed respectively (is collected in Yanbian University Hou Shan, Heilungkiang blackland and Hunchun respectively
Chuan Shaqiu；Cross the pharmacopeia that aperture is 2mm to sieve, lower part of screen point taken to use) it is put into 20mL sample bottle, it is sequentially added into fluoranthene mark
Quasi- 2.5 μ L of solution (2.0000mg/mL), 5 μ L, 10 μ L, 16.25 μ L, 20 μ L, 25 μ L carry out the pollution of 1~10mg/kg gradient, often
5mL bioactive water (BW), ultrapure water (UPW), distilled water (DW), mineral water (being derived from spring Yangquan) are separately added into a sample bottle
And tap water, gained Soil slurry sample is placed in progress Balance Treatment 48h on desk-top roller bottle apparatus；Later by gained system
It is separated by solid-liquid separation, obtains water phase and solid phase, the concentration of fluoranthene in water phase and solid phase is analyzed using HPLC, wherein HPLC analysis
Condition includes:
Liquid-phase chromatographic column: ZORBAX Eclipse PAH (4.6mm × 250mm, 5 μm)；
Column temperature: 35 DEG C；
Mobile phase: acetonitrile is 9:1 with ultrapure water volume ratio；
Flow velocity: 1.0mL/min；
UV Detection wavelength: 220nm；
Sample volume: 10 μ L.
Fig. 2 is distribution coefficient comparison diagram in fluoranthene gradient contaminated soil mud sample, as shown in Figure 2, soil made of 5 kinds of water
The distribution coefficient (Kd) of fluoranthene is successively in earth mud sample are as follows: BW < UP < DW < mineral water < tap water illustrates in bioactive water
The Kd value of fluoranthene is minimum, strong to the desorption ability of organic matter, and the utilizability of microorganism can be enhanced, promote the drop of microorganism
Solution.
4, polycyclic aromatic hydrocarbons contaminated general soil is carried out using bioactive water biological prosthetic
Experimental group: 5g loess, black earth and sandy soil are weighed respectively and (are collected in Yanbian University Hou Shan, Heilungkiang blackland respectively
With Hunchun Chuan Shaqiu；Cross the pharmacopeia that aperture is 2mm to sieve, lower part of screen point taken to use) it is put into 20mL sample bottle, it is separately added into 5 μ
L fluoranthene standard solution (2.0000mg/mL) makes fluoranthene pollution concentration 2mg/kg in soil, spare as soil to be repaired；
Soil, 5mL bioactive water and the nutrient matrix (MSM) to be repaired are mixed, gained mixed material is placed
In on desk-top roller bottle apparatus, being balanced processing 48h under room temperature, Soil slurry is obtained；
The Soil slurry is inoculated with degradation bacteria YN1 (deposit number CGMCC No.:17911, inoculum concentration 2%, v/v),
It is placed on desk-top roller bottle apparatus later, carries out biodegrade 48h under room temperature.
Setting control group: Soil slurry, other conditions and experimental group method are prepared using distilled water substitution bioactive water
Unanimously.
Fluoranthene concentration remaining in gained system after biodegrade in experimental group and control group is analyzed using HPLC, is obtained not
With the biological degradation rate of fluoranthene in pedotheque, it is specifically shown in Table 1.
The biological degradation rate of fluoranthene in 1 different soils of table
As shown in Table 1, compared with control group (soil+DW), three kinds of Soil slurries equal all table in experimental group (soil+BW)
Reveal the phenomenon that degradation rate improves, illustrates that bioactive water plays facilitation to the biological prosthetic of polycyclic aromatic hydrocarbon pollution.
In the test of three kinds of Soil slurries, for control group, role is most in Loess Samples experiment for the bioactive water of experimental group
Height, 70% or more increase rate of degrading.
5, biology is carried out to 6 kinds of PAHs (respectively naphthalene, fluorenes, phenanthrene, anthracene, fluoranthene and pyrene) contaminated soil using degradation bacteria YN1
It repairs
According to above-mentioned experimental method, loess is polluted using 6 kinds of PAHs, and Soil slurry is made and carries out biodegrade
Experiment, concrete outcome are as shown in Figure 3.From the figure 3, it may be seen that degradation bacteria YN1 has degrading activity to 6 kinds of PAHs such as naphthalene, fluorenes, anthracene, and
BW is improved effect to the degradation of PAHs.
6, the influence that different salt concentration conditions grow degradation bacteria YN1
Addition NaCl prepares the culture medium of 0~15% different salinity in nutrient matrix (MSM), adds 0.1% (w/
V) fluoranthene is inoculated with the degradation bacteria YN1 of 1% (v/v), shaking table shaken cultivation under the conditions of 37 DEG C, 120r/min as carbon source
36h measures OD600, concrete outcome is as shown in Figure 4.As shown in Figure 4, the suitable growth salt concentration range of degradation bacteria YN1 be 2~
6%, most suitable salt concentration conditions are 4%, can be survived under the conditions of salinity is 0~15%.
7, polycyclic aromatic hydrocarbons contaminated saline-alkali soil is carried out using bioactive water biological prosthetic
A certain amount of saline-alkali soil (salinity 10%) is taken in certain salt-soda soil, is diluted to crude salt alkaline earth volume respectively using DW
2.5 times and 1.5 times, quicksand like saline-alkali soil is prepared, then with dilution 2.5 times, dilution 1.5 times and undiluted saline-alkali soil
As pedotheque, adopted by the method in " 4, using bioactive water carried out to polycyclic aromatic hydrocarbons contaminated general soil biological prosthetic "
It is polluted with fluoranthene, obtains saline-alkali soil to be repaired；
The ratio for being 1g:1mL in pedotheque and bioactive water, adds bioactive water, at room temperature respectively
It is balanced processing 48h, obtains Soil slurry, i.e., salinity is respectively that 2%, 4% and 5% is not (dilute in gained Soil slurry
Release), inoculation degradation bacteria YN1 carries out biodegradable experiment later, uses distilled water according to experimental group situation setting in table 2, surpasses
Pure water and the control experiment for adding nutrient matrix, are as a result specifically shown in Table 2.
The biological degradation rate of fluoranthene in the saline-alkali soil of the different salinity of table 2
As shown in Table 2, under the conditions of the Soil slurry of 3 kinds of different dilution saline-alkali soil, fluoranthene degradation rate is BW > BW+
MSM > UPW+MSM > DW+MSM > UPW > DW, this illustrates that bioactive water (BW) can effectively improve fluoranthene in saline-alkali soil
In degradation efficiency.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of restorative procedure of polycyclic aromatic hydrocarbon pollution, which comprises the following steps:
Polycyclic aromatic hydrocarbon pollution and bioactive water are mixed, Soil slurry is obtained；
Biodegrade is carried out after the Soil slurry is inoculated with polycyclic aromatic hydrocarbon-degrading bacteria.
2. restorative procedure according to claim 1, which is characterized in that the raw material for preparing the Soil slurry further includes nutrition
Matrix.
3. restorative procedure according to claim 1, which is characterized in that salt content in the polycyclic aromatic hydrocarbon pollution≤
15%.
4. restorative procedure according to claim 3, which is characterized in that the soil type packet of the polycyclic aromatic hydrocarbon pollution
Include loess, black earth or sandy soil.
5. restorative procedure according to any one of claims 1 to 4, which is characterized in that salt content in the Soil slurry≤
12%.
6. restorative procedure according to claim 5, which is characterized in that salt content≤6% in the Soil slurry.
7. restorative procedure according to claim 1 or 2, which is characterized in that the inoculum concentration of the polycyclic aromatic hydrocarbon-degrading bacteria is 1
~10%.
8. restorative procedure according to claim 1 or 2, which is characterized in that the biodegradable temperature is 15~45 DEG C,
Time is 1~15d.
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Cited By (3)
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| CN113458138A (en) * | 2021-07-07 | 2021-10-01 | 合肥学院 | Experimental method for repairing fluorene contaminated soil |
| CN115232625A (en) * | 2021-09-30 | 2022-10-25 | 黄芸 | Treatment method and application of soil organic pollutants |
| CN115532802A (en) * | 2022-10-20 | 2022-12-30 | 生态环境部南京环境科学研究所 | Method for degrading and repairing PAHs in polluted soil |
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