CN110498718A - A kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio and preparation method thereof - Google Patents
A kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of Penicillium notatums, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio and preparation method thereof.The organic phosphorus fertilizer of compound bio, it is to puddle drying by cultural hypha, the decomposed harmless treatment of animal waste, addition ground phosphate rock three respectively using lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1, animal waste and calcium phosphate as raw material and be made；Wherein, its active constituent is the mycelia of Ji agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 CGMCC No.17668.The organic phosphorus fertilizer dissolving P capacity of compound bio is strong, can decompose the indissoluble P elements around crop root.Wheat bran can be used, the agricultural wastes such as husk, which cultivate as raw material preparation culture medium, can obtain a large amount of thallus, so that production cost is reduced, and the technological process of production is simple, it is suitable for large-scale promotion.
Description
Technical field
The invention belongs to microbial manure technical field more particularly to the compound lifes of a kind of Penicillium notatum, animal waste, calcium phosphate
Organic phosphorus fertilizer of object and preparation method thereof.
Background technique
Phosphorus is one of mineral element necessary to plant growth, is plant nucleic acid in vivo and a variety of enzymes, coenzyme, Adenosine triphosphate
The important component of glycosides (ATP) etc., and the intracorporal photosynthesis of plant and physiological and biochemical procedure are participated in many ways, it is right
The growth and development and metabolism for promoting plant play a significant role.Plant, which lacks phosphorus, will affect nucleic acid, protein synthesis, make cell
It is formed and proliferation is suppressed, growth and development is caused to be stagnated, root system development is bad, and plant is short and small, and all kinds of crop tillers will be reduced
Or delay, heading are postponed, are bloomed late, mature grain number per spike is reduced, and hollow kernels, corn ear is bald late, and potato tubers becomes
It is small etc..Therefore, phosphorus is very important for agricultural production.
The phosphorus library of nature is primarily present in soil, and circulation mainly carries out between soil, plant and microorganism.Soil
The height of supply source of the earth as plant phosphorus element, Phosphorus-supplying Level affects the growth and development of plant.Phosphorus in soil is containing organic
Phosphorus and two kinds of Phos, most of is insoluble phosphate (calcium phosphate, ferric phosphate, aluminate or phosphate etc.), these forms it is inorganic
Phosphide is difficult to be absorbed and used by plants, can only be accounted for by the available P that plant is directly absorbed and utilized in soil full phosphorus 2%~
3%.Most of phosphorus forms insoluble phosphorus in conjunction with Ca2+, Fe3+, Fe2+, Al3+ in soil due to the fixed function of soil
Hydrochlorate, therefore, most of phosphate fertilizer accumulate in the soil as invalid state (indissoluble state).Due to long-term application of P fertilizer, in fact greatly
Most potential phosphorus library contents of agricultural land soil are very big, and phosphate fertilizer excessive use will cause the increase of heavy metal content in soil, soil
Earth physicochemical property changes, and a large amount of applications of organophosphorus pesticide, causes environmental pollution, influences agricultural product quality, is good for the mankind
Health brings very big harm.
Phosphorus is the important limiting factor in many developing country's agricultural productions, and the utilization efficiency for improving phosphorus in soil will have
It is of great importance.The experimental results show that certain micro-organisms has the function of very strong phosphorus decomposing, by its secretion or absorb work
With that unavailable phosphorus in soil can be converted to available P, the material incentives plant growths such as auxin are also secreted.Phosphate solubilizing microorganism
Most direct effect is exactly slightly solubility or the insoluble phosphorus element dissolved in soil.Therefore, phosphate solubilizing microorganism is screened, production contains solution
The microbial organic fertilizer of phosphorus function is a good approach to plant phosphorus element supply problem is solved.
Phosphate solubilizing microorganism is divided into phosphate-solubilizing bacteria, phosphorus decomposing fungi and phosphorus decomposing actinomyces.Currently, majority is raw about phosphate-solubilizing bacteria
The research of object fertilizer, and it is relatively fewer about phosphorus decomposing fungal organism fertilizer；The shortcomings that phosphobacteria bio-fertilizer: (1) for cultivating phosphorus
Stringent sterile working must be carried out in bacteria culture media and incubation, be even so also easy by other fungal contaminations；(2)
Phosphobacteria easily it is affected by environment mutation and lose phosphorus decomposing effect；(3) C/N ratio must be suitable for during the cultivation process, otherwise shadow
Ring growth.(4) second level culture and third stage culture can only fluid nutrient medium culture, production technology is complicated, increases production cost.And
Phosphorus decomposing fungi knows that bacterium volume is smaller according to the characteristics of microorganism, easy mutation affected by environment, and fungi has
Genetic stability, and the feature that dissolving P capacity is strong.Currently, isolated phosphorus decomposing fungi mainly include mould, aspergillus, head mold,
Reaping hook is mould, small rhizoctonia etc., wherein reporting that more is aspergillus niger and mould.
Summary of the invention
In view of the above-mentioned state of the art, the present invention provides a kind of Penicillium notatum, animal waste, calcium phosphate compound bios
Organic phosphorus fertilizer and its preparation method and application, to solve drawbacks described above existing in the prior art.
The technical scheme of the invention to solve the technical problem is:
1. the preparation method of a kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio: with lucky agriculture Penicillium notatum
(Penicillium chinensis) TYPS-1, animal waste and calcium phosphate are raw material, pass through cultural hypha, family's animal manure respectively
Just decomposed harmless treatment, addition ground phosphate rock three puddle drying；Wherein,
Cultural hypha:
Described Ji agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia is prepared by following preparation method
It arrives:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum
A small amount of mycelium is taken to cross on inclined-plane in (Penicillium chinensis) TYPS-1CGMCC No.17668 original seed, so
It cultivates at 28 DEG C afterwards, covers with inclined-plane；The PDA solid medium is made of following component based on mass fraction: Ma Ling
Potato 20g, glucose 2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table
Culture；The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone
0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands
Big solid medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands
When solid culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder
3%, sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level
Expand solid medium to be made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water are material weight
60%, pH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly, are made lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia object
Material；
The decomposed harmless treatment of animal waste:
(1) pre-treatment: because animal waste water content is high, while in order to adjust its C/N to 30:1, Yao Tianjia 25~
35% straw powder and (or) powdered rice hulls and/or sawdust etc. reduce moisture, increase gas permeability.
(2) one time fermentation: providing oxygen by the material of turning or forced ventilation into heap body or in installation for fermenting, so as to
In material under the action of microorganism aerobic fermentation, while preventing temperature excessively high, after a period of time, most of organic matter is dropped
Solution, various harmful organisms are killed, heap temperature decline.
(3) secondary fermentation: after one time fermentation, the degradable organic substance of major part in compost material is micro-
Biodegrade, but some degradable and a large amount of organic matter difficult to degrade exists, it further need to ferment, be allowed to become
The more stable organic matter such as humic acid, amino acid reaches decomposed；It spontaneously dries and obtains after the material deodorization of secondary fermentation
Animal waste material after decomposed harmless treatment.
Addition ground phosphate rock three puddles drying and finished product is made:
Animal waste material after decomposed harmless treatment adds ground phosphate rock in 2/1000 ratio or calcium phosphate mixes
It is even；Lucky agriculture Penicillium notatum (Penicillium chinensis) the TYPS-1 mycelia kind of drying is puddled in 5/10000 ratio addition
Absorption, then dries pulverizing, is made the organic phosphorus fertilizer of the compound bio.
2. a kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio, its active constituent is lucky agriculture mould
The mycelia of bacterium (Penicillium chinensis) TYPS-1CGMCC No.17668.
3. a kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio, the organic phosphorus fertilizer of compound bio
It is prepared by the preparation method of a kind of Penicillium notatum, animal waste described in technical solution 1, the organic phosphorus fertilizer of calcium phosphate compound bio
It obtains.
4. lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 drop described in technical solution 1 or 2 or 3
Solve the application in indissoluble Phos.
Compared with prior art, the invention has the following advantages:
The present invention is with lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1CGMCC No.17668, family's animal manure
Just it is raw material with calcium phosphate, puddles baking by cultural hypha, the decomposed harmless treatment of animal waste, addition ground phosphate rock three respectively
It is drying at can improve the utilization rate of the biological effectiveness of insoluble phosphorus and soil phophorus in soil, save fertilizer volume increase, improve soil ring
Border, can effectively facilitate plant includes absorption of the crops to soil P elements, decomposes the indissoluble P elements around crop root, mentions
High grain quality；It partially or largely can replace chemical phosphatic ferfilizer and directly apply to production estimation；Improve soil environment, reduces
Environmental pollution.
In addition, needing to carry out culture medium in sterilizing and incubation before culture according to the Biological characteristics of microorganism
Sterile working is carried out, and the likelihood ratio bacterium that Penicillium notatum is polluted by other bacterium is low；Since Penicillium fungi is adaptable, poor
It can be mushroomed out in lean nutrition, so when three-level expands solid culture wheat bran can be used, the agricultural wastes such as husk are as raw material
Preparation culture medium, which cultivate, can obtain a large amount of thallus, to reduce production cost, and the technological process of production is simple, fit
Close a wide range of promote.
Detailed description of the invention
Fig. 1 is schematic diagram of the present invention.
Specific embodiment
, there are further understanding and understanding in the effect of to make to feature of the invention and being reached, preferably to implement
Example and attached drawing cooperation detailed description, each embodiment is conventional method without specified otherwise, is described as follows:
The Penicillium notatum of the present invention of embodiment 1, animal waste, the Penicillium notatum description in the organic phosphorus fertilizer of calcium phosphate compound bio:
Mould (Penicillium sp.) TYPS-1
Referred to as: CGMCC
Deposit number: CGMCC No. 17668
Preservation date: on May 15th, 2019
The title of depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation address: city, BeiJing, China, North Star West Road 1, Chaoyang District institute 3
Postcode: 100101
Ji agriculture Penicillium notatum (Penicillium chinensis) TYPS-1, which is used, contains indissoluble phosphorus culture medium, in pH9.0
Under the conditions of separate and obtain from the milpa soil of environment in Tongyu County of Jilin Province.
Morphological analysis: having conidial head, generates conidium, and conidiophore betides aerial hyphae, mitogenetic spore
Son stalk, metulae and conidial wall are significantly coarse, and most of broom shape branch is raw for two-wheel, have small part three verticillate and non-
Symmetrical, metulae is snugly into each other.Bacterium colony is cotton-shaped and velvet-like in PDA and CYA solid medium quality, and conidium structure is usual
Rareness, the white mycelium or pistac at edge, middle part are in celadon.
The preparation of embodiment 2 Penicillium notatum of the present invention, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio
The preparation method of Penicillium notatum of the present invention, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio: with lucky agriculture Penicillium notatum
(Penicillium chinensis) TYPS-1, animal waste and calcium phosphate are raw material, pass through cultural hypha, family's animal manure respectively
Just decomposed harmless treatment, addition ground phosphate rock three puddle drying；Wherein,
Cultural hypha:
Described Ji agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia is prepared by following preparation method
It arrives:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum
A small amount of mycelium is taken to cross on inclined-plane in (Penicillium chinensis) TYPS-1CGMCC No.17668 original seed, so
It cultivates at 28 DEG C afterwards, covers with inclined-plane；The PDA solid medium is made of following component based on mass fraction: Ma Ling
Potato 20g, glucose 2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table
Culture；The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone
0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands
Big solid medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands
When solid culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder
3%, sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level
Expand solid medium to be made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water are material weight
60%, pH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly, are made lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia object
Material；
The decomposed harmless treatment of animal waste:
(1) pre-treatment: because animal waste water content is high, while in order to adjust its C/N to 30:1, Yao Tianjia 25~
35% straw powder and (or) powdered rice hulls and/or sawdust etc. reduce moisture, increase gas permeability.
(2) one time fermentation: providing oxygen by the material of turning or forced ventilation into heap body or in installation for fermenting, so as to
In material under the action of microorganism aerobic fermentation, while preventing temperature excessively high, after a period of time, most of organic matter is dropped
Solution, various harmful organisms are killed, heap temperature decline.
(3) secondary fermentation: after one time fermentation, the degradable organic substance of major part in compost material is micro-
Biodegrade, but some degradable and a large amount of organic matter difficult to degrade exists, it further need to ferment, be allowed to become
The more stable organic matter such as humic acid, amino acid reaches decomposed；It spontaneously dries and obtains after the material deodorization of secondary fermentation
Animal waste material after decomposed harmless treatment.
Addition ground phosphate rock three puddles drying and finished product is made:
Animal waste material after decomposed harmless treatment adds ground phosphate rock in 2/1000 ratio or calcium phosphate mixes
It is even；Lucky agriculture Penicillium notatum (Penicillium chinensis) the TYPS-1 mycelia kind of drying is puddled in 5/10000 ratio addition
Absorption, then dries pulverizing, is made the organic phosphorus fertilizer of the compound bio.
Lucky agriculture Penicillium notatum (Penicillium chinensis) the TYPS-1 dissolving P capacity test of 3 present invention of embodiment
A small amount of strain is seeded in first after cultivating a certain concentration in 100mLPDA fluid nutrient medium, by 2% inoculum concentration
It is inoculated into containing indissoluble phosphorus Ca3(PO4)2Fluid nutrient medium culture 1d after, with molybdenum antimony resistance colorimetric method survey strain phosphorus decomposing amount；Knot
Fruit, compared with the control group for not connecing bacterium, when original fluid pH7.0, measuring titanium pigment content is 280.261 ± 1.11 μ g/mL；
As original fluid pH9.0, measuring titanium pigment content is 331.843 ± 3.41 μ g/mL.According to document announcement, under equal conditions,
Phosphorus decomposing amount of phosphobacteria 3-4d up to 600 μ g/mL or so, it is therefore seen that the dissolving P capacity of this Penicillium notatum is strong.
It should be noted that relational terms such as first and second and the like are used merely to an entity herein
Or operation is distinguished with another entity or operation, is existed without necessarily requiring or implying between these entities or operation
Any actual relationship or order.And the terms "include", "comprise" or its any other variant are intended to non-row
His property includes, so that the process, method, article or equipment for including a series of elements not only includes those elements, and
And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic
Element.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of skill in the art that it still can be right
Technical solution documented by previous embodiment is modified, or is replaced on an equal basis to part of technical characteristic；And these
It modifies or replaces, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (2)
1. a kind of Penicillium notatum, animal waste, the organic phosphorus fertilizer of calcium phosphate compound bio, it is characterised in that: it is with lucky agriculture mould
Bacterium (Penicillium chinensis) TYPS-1, animal waste and calcium phosphate are raw material, pass through cultural hypha, domestic animal respectively
The decomposed harmless treatment of excrement, addition ground phosphate rock three puddle drying and are made；Wherein, its active constituent is lucky agriculture Penicillium notatum
The mycelia of (Penicillium chinensis) TYPS-1 CGMCC No.17668, the mycelia are prepared by following preparation method
It obtains:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum (Penicillium
Chinensis it) takes a small amount of mycelium to cross on inclined-plane in TYPS-1 CGMCC No.17668 original seed, then cultivates at 28 DEG C,
Cover with inclined-plane；The PDA solid medium is made of following component based on mass fraction: potato 20g, glucose
2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；
The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone 0.2%,
Potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands admittedly
Body culture medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands solid
When culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder 3%,
Sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level expands
Solid medium is made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water be expect weight 60%,
PH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly, are made lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia material；
The decomposed harmless treatment of animal waste:
(1) pre-treatment: because animal waste water content is high, while in order to adjust its C/N to 30:1, Yao Tianjia's 25~35%
Straw powder and (or) powdered rice hulls and/or sawdust etc. reduce moisture, increase gas permeability；
(2) one time fermentation: oxygen is provided by the material of turning or forced ventilation into heap body or in installation for fermenting, in order to object
Material aerobic fermentation under the action of microorganism, while preventing temperature excessively high, after a period of time, most of organic matter is degraded,
Various harmful organisms are killed, heap temperature decline；
(3) secondary fermentation: after one time fermentation, the degradable organic substance of major part in compost material is by microorganism
Degradation, but some degradable and a large amount of organic matter difficult to degrade exists, it further need to ferment, be allowed to become humic
The more stable organic matter such as acid, amino acid reaches decomposed；Natural drying obtains decomposed after the material deodorization of secondary fermentation
Animal waste material after harmless treatment；
Addition ground phosphate rock three puddles drying and finished product is made:
Animal waste material after decomposed harmless treatment adds ground phosphate rock in 2/1000 ratio or calcium phosphate is uniformly mixed；
Lucky agriculture Penicillium notatum (Penicillium chinensis) the TYPS-1 mycelia kind of drying puddles suction in 5/10000 ratio addition
Attached, then dries pulverizing, is made the organic phosphorus fertilizer of the compound bio.
2. a kind of lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 mycelia kind according to claim 1 exists
Application in degradation indissoluble Phos.
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Cited By (1)
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| CN112280563A (en) * | 2020-10-28 | 2021-01-29 | 福建龙净环保股份有限公司 | Phosphate solubilizing bacteria mineralization conditioner, preparation method thereof and heavy metal contaminated soil remediation and improvement method |
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