CN110470601A - Water quality many reference amounts detection device and detection method based on chip reaction unit - Google Patents
Water quality many reference amounts detection device and detection method based on chip reaction unit Download PDFInfo
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- CN110470601A CN110470601A CN201810449279.XA CN201810449279A CN110470601A CN 110470601 A CN110470601 A CN 110470601A CN 201810449279 A CN201810449279 A CN 201810449279A CN 110470601 A CN110470601 A CN 110470601A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
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Abstract
The present invention relates to microchip fields, disclose a kind of based on the water quality many reference amounts detection device and detection method of chip reaction unit and its application.The water quality many reference amounts detection device includes chip reaction unit, which includes manual injector and chip；The chip includes runner, reaction tank and injection port, is connected to reaction tank with injection port by the runner；The manual injector includes sample cell, control unit and sample portion out, the control unit is connected with the sample cell, the sample cell is released for entering sample and being maintained in the sample cell or sample is made to go out sample portion described in, the sample portion out is located at the side opposite with the control unit of the sample cell；The injection port of the manual injector and the chip is tightly connected.Water quality many reference amounts detection device of the invention can accurately control the type of flow of sample, and the multiple functions such as sampling, mixing, driving can be realized manually, simplify device, convenient for providing the controllability and repeatability of operation.
Description
Technical field
The present invention relates to microchip fields, and in particular to a kind of water quality many reference amounts detection device based on chip reaction unit
With detection method and its application.
Background technique
One of developing direction of modern age instrument is miniaturization, integrated, portability.No matter in life science, chemical
The appearance of field or field of environment protection, micro-total analysis system (microTAS) has had resulted in revolutionary variation.It is micro- complete
The purpose of analysis is that function is integrated, sample preparation, separation, purifying, the reaction, prison that will need to realize in laboratory originally
The integration of the sophisticated functions such as survey focuses on the micro analytical device even chip of credit card-sized, therefore is also known as " chip reality
Test room ".
Micro-total analysis technology is proposed the nineties in last century by Manz etc., has benefited from the phases such as micro electro mechanical processing, biochemistry
The development of pass technology has been increasingly becoming the cutting edge technology of analytical chemistry.It is with a series of spies for being different from traditional technology
Point: 1) effective heat and mass and characteristic dimension is small, thus processing speed is fast, high-efficient；2) reagent loss is small；3) equipment size
Small, light-weight, function integrated-type height；4) individual equipment is at low cost when producing in enormous quantities.It is widely applied rapidly in these advantages
In fields such as drug screening, high-flux sequence, molecular diagnosis, environment monitorings.Wherein, micro-total analysis technology is with its portability and function
Energy integration is widely used in on-site test, and the scene that can not be popularized or be limited for laboratory test provides solution.
What the realization of each function relied on is the flowing of drop or liquid therein in micro-total analysis chip, it is therefore necessary to by outer
Boundary provides certain driving force, promotes sample to enter chip interior by outside, and portion flows through separation, reaction, detection in the chip
Equal modules.Sampling technique directly controls the realization of analytic process, is the important component of micro-total analysis technology.
The fluid actuation techniques of micro-total analysis chip are broadly divided into two classes, mechanical power drive and on-mechanical power drive at present.
The former drives fluid by the movement of system own mechanical component, drives comprising centrifugal force such as external impetus, the Lab CD such as syringe pump
Dynamic, Pneumatic Micropump, piezoelectric micropump etc., latter system itself is free of the mechanical part of movement, comprising driven by electroosmosis, gravity drive,
Electric hydro powered etc..Related patents document includes:
CN103894128A discloses a kind of automatic reaction chip of piezoelectric type, is equipped with several layers from top to bottom, is followed successively by micro- mixed
Clutch layer, the first insulating layer, transducer layer and second insulating layer, are wherein integrated with vibration mixing pit in micro-mixer layer, and first
Insulating layer is located between micro-mixer layer and transducer layer, and interdigital transducer and chip electrode are integrated in transducer layer, fork
Finger-type energy converter is located at the underface of vibration mixing pit, and interdigital transducer is corresponding to the vibration shape of mixing pit, vibration mixing
Fluid in pond vibrates under the action of interdigital transducer, and interdigital transducer is connected with chip electrode, chip electrode with
Response controller be connected, response controller is controlled by plug-in, be it is a kind of integrate mixing, reaction, temperature control, stirring it is more
The novel micro fluid reaction chip of function full automatic；The scope of application of micro fluid reaction chip is greatly improved, and is not only limited to
In micro- biochemical reaction under room temperature.
CN104832404A discloses a kind of piezoelectric micropump based on PDMS.The piezoelectric pump, by piezoelectric vibrator, valve block,
Hard washer and PDMS pump housing composition, piezoelectric vibrator generate bending vibration up and down under the action of alternate electrical signal, cause cavity
The pumping of fluid is realized in the variation of volume and pressure under the mating reaction of check valve sheet.And hard washer is embedded in the pump housing,
Piezoelectric vibrator is assemblied in washer inner ring, and piezoelectric vibrator is avoided to be fit directly into the volume change on PDMS elastomer and influencing cavity
Change amount.Piezoelectric pump micro-pump structure based on PDMS can be the diversified forms such as single-chamber, double series-wound chambers, double-cavity parallel, multi-cavity.It adopts
The liquid of certain special natures can not be pumped by solving current piezoelectric micropump with the PDMS material production piezoelectric micropump pump housing, not had
The problems such as having good transparency, can not being integrated in micro-fluidic chip, has expanded the application field of piezoelectric micropump, even more greatly
Solve the problems, such as micro-fluidic field integrated chip driving source.
What CN107020165A disclosed a kind of gravity drive integrates sculptured micro-fluidic chip and its application, belongs to micro-
Fluidics field by multiple surfaces there is the chip of sculptured structure to be spliced by splicing mouth；The embossment declines
The structure of fluidic chip has eight kinds of transmission, screening, splitting, fusion, concussion, jump, replacement and collection by function point.The structure
The microfluidic channel structure with certain special exercise TRAJECTORY CONTROL function and the splicing mouth structure of outer are contained, by by core
Piece carries out flexibly assembly according to set purpose and integrates, in combination with the modifying super hydrophobicity of gravity drive and chip surface, Ke Yishi
Now high speed, multichannel and functional motion profile is carried out to drop to control.
CN102784672A be related to it is a kind of detect NO3-N and NO2-N centrifugal type microfludic chip and its preparation side
Method.The chip is centrifugal type microfludic chip of the disc-shaped with micro-structure and microchannel, in the centrifugal force that centrifuge rotation generates
Under driving, sample and the mixing of reagent completed, reacted and separation process, with ultra-violet and visible spectrophotometer is quantitative or qualitative inspection
Measure the content of NO3-N and NO2-N in sample.The centrifugal type microfludic chip needs reagent few with sample, is not necessarily to sample introduction
Pre-treatment can handle and detect simultaneously multiple samples, realize the integrated of detection, micromation, automation, have economical, fast
Fast, portable, efficient feature, the rapid quantitative detection for water body, soil and Nitrate Content in Food and nitrite provide one
The completely new analytical technology of kind.
But above-mentioned technology all has certain problems when being applied to on-site test.External mechanical pressure-driven and electricity
It seeps driving to generally require to provide corresponding auxiliary device, violates the requirement of portable devices, micromation；Although Micropump drives volume
It is small but very high to machining accuracy requirement, realize that difficulty is big, and technology is not mature enough；Gravity drive is not necessarily to exterior power, but right
Fluid controllable is poor, and is not suitable for microscale channel (surface tension effects are better than gravity).Therefore, one kind is needed at present
It can be applied to the reliable sample introduction driving method of on-site test.
Summary of the invention
The purpose of the invention is to overcome the above problem of the existing technology, provide a kind of based on chip reaction unit
Water quality many reference amounts detection device and detection method and its application, the chip reaction unit can accurately control the flowing side of sample
The multiple functions such as sampling, mixing, driving can be realized manually, simplify device for formula, convenient for providing the controllability of operation and can weigh
Renaturation.
To achieve the goals above, one aspect of the present invention provides the water quality many reference amounts detection based on chip reaction unit and sets
Standby, which includes chip reaction unit, which includes manual injector and chip；Institute
Stating chip includes runner, reaction tank and injection port, and is connected to the reaction tank with the injection port by the runner；It is described
Manual injector includes sample cell, control unit and sample portion, the control unit are connected with the sample cell out, for make sample into
Enter and be maintained in the sample cell or sample is made to release the sample cell through the sample portion out, the sample portion out is located at described
The side opposite with the control unit of sample cell；The injection port of the manual injector and the chip is tightly connected.
Preferably, the injection port of sample portion and chip out of the manual injector is removably tightly connected；Alternatively, described
The sample portion that goes out of manual injector is fixedly connected with the injection port of the chip.
Preferably, the control unit and the sample cell are detachably connected.
Preferably, the sample cell and the control unit are cylindricality.
Preferably, the control unit is screwed on the sample cell.
Preferably, the sample cell and control unit are tube-in-tube structure.
Preferably, the inside of the sample cell is arranged in the control unit.
Preferably, the lateral wall bottom of the control unit is provided with sealing ring, and the sealing ring is for sealing the control
Gap between portion and the sample cell.
Preferably, the control unit have can deformation part, and by this can the deformation of deformation part change the sample
The volume of pond inner cavity；
It is highly preferred that the control unit can deformations be divided into the scalable side wall of alterable height.
Preferably, the sample cell is transparent or semitransparent sample cell.
Preferably, the material of the sample cell be polyethylene, polypropylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene (PTFE),
One of polycarbonate, polymethyl methacrylate, ABS plastic, phenolic resin, epoxy resin, glass and quartz are a variety of,
The control unit can the material of deformation part be one of polyethylene, polypropylene, polytetrafluoroethylene (PTFE) and polyvinyl chloride or more
Kind.
Preferably, the bottom in the sample portion out has helicitic texture or flexible seal member；
Preferably, the helicitic texture is internal thread structure or external thread structure；
Preferably, the flexible seal member is rubber stopper.
Preferably, which further includes more than one detection part；It is highly preferred that the test section
Part is chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman signal detection component and infrared spectroscopy inspection
Survey one of component or a variety of.
Preferably, the water quality many reference amounts detection device further include drive chip rotation driving part, signal acquisition component,
One of data processor, data output component and data transfer component are a variety of.
Second aspect of the present invention provides a kind of water quality many reference amounts detection method, wherein this method comprises: keeping above-mentioned water quality
Many reference amounts detection device manual injector and chip separation in the state of, operate the control unit of manual injector, make sample into
Enter and be maintained in the sample cell, the manual injector is then gone out into the sealed connection in sample portion and the chip, then grasp
The control unit for making manual injector makes sample release through the sample portion out or enter back into the sample cell, to control sample in institute
State the flowing in chip.
Third aspect present invention provides above-mentioned water quality many reference amounts detection device or above-mentioned water based on chip reaction unit
Application of the matter many reference amounts detection method in fields such as analytical chemistry, life sciences.Specifically, water quality many reference amounts of the invention detection
Equipment or above-mentioned water quality many reference amounts detection method can be applied in fields such as environmental monitoring, Emergent detection or molecular diagnosises.
Through the above technical solutions, water quality many reference amounts detection device of the invention and method have the advantages that
(1) it can control sample two-way flow, allow to realize on chip more multi-functional, such as sample is in reaction tank
In mixing or the flowing between differential responses pond；
(2) manual injector can separate, while use as sampler；
(3) cavity volume of sample cell can be accurately determined according to chip microchannel volume, pressing or extraction are to chip every time
The pressure or negative pressure generated in microchannel is certain, and realization accurately controls fluid, flexible modulation can react opportunity according to demand；
(4) it can be driven manually, be not necessarily to external connection power, it is low in cost because without auxiliary device, structure is simple, especially
Suitable for field assay.
(5) it is not necessary that the buffer cavity for storing fluid sample and air is arranged on chip, chip structure is simplified, is saved
Save chip volume；
(6) direct contact type, screw-type, flexible closure formula can be used in the connector of sample introduction head and chip, and scheme is flexible, can root
According to needing to select.
Detailed description of the invention
Fig. 1 is the schematic diagram of the chip reaction unit of an embodiment of the invention；
Fig. 2 is the schematic diagram of the chip reaction unit of another embodiment of the invention；
Fig. 3 is the schematic diagram of the chip reaction unit of another embodiment of the invention.
Description of symbols
1, manual injector 2, chip
11, control unit 12, sample cell 13, out sample portion
21, runner 22, reaction tank 23, injection port
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
Water quality many reference amounts detection device provided by the invention based on chip reaction unit, the water quality many reference amounts detection device
Including chip reaction unit, wherein the chip reaction unit includes manual injector 1 and chip 2；The chip 2 includes runner
21, reaction tank 22 and injection port 23, and be connected to the reaction tank 22 with the injection port 23 by the runner 21；The hand
Dynamic sample injector 1 includes sample cell 12, control unit 11 and sample portion 13, the control unit 11 are connected with the sample cell 12 out, uses
In enter sample and be maintained in the sample cell 12 or make sample through it is described go out sample portion 13 release the sample cell 12, institute
State out the side opposite with the control unit 11 that sample portion 13 is located at the sample cell 2；The manual injector 1 and the chip
2 injection port 23 is tightly connected.
In the present invention, the manual injector 1 can cooperate with the chip 2, realize sample introduction and control sample and exist
The purpose of flow process in the chip 2, the manual injector 1 can be used for sampling, thus the very big behaviour simplified
Make the device needed, is highly convenient for the use of sampling device under various application scenarios.Flowing of the above-mentioned control sample in chip 2
Process, while the process including pushing sample to move forward or back, so as to so that the function of chip is more diversified, such as can be with
Sample is set to iterate through some reaction tank to accelerate the mixing of sample, or after first mixing with the sample in some chamber, then
It is mixed with the sample of other reaction tank.
In the present invention, there is no particular limitation for the structure of the chip 2, as long as having above-mentioned runner 21, reaction tank
22 and injection port 23, specific quantity, position and mutual connection relationship can be had according to the experiment of required progress
Body determines.Such as chip 2 is when including multiple reaction tanks 22, and injection port 23 and each anti-can be separately connected by multiple runners 21
Pond 22 is answered, injection port 23 and each reaction tank 22 can also be sequentially connected by multiple runners 21, sample is entered
Each reaction tank 22.In addition, as needed, injection port 23 can certainly be set as multiple, and corresponding each injection port
The corresponding runner 21 of 23 settings, reaction tank 22.
Manual injector 1 of the invention can be used cooperatively with various types of micro-fluidic chips.As needed, it is described into
Sample mouth 23 can protrude from the outside of chip, also can be set in the inside of chip, and the preferably described injection port 23 is provided with screw thread,
To be screwed on sample portion 13 out.
According to the present invention, the manual injector 1 can be according to different hand samplings from the connection relationship of the chip 2
Device is selected.
A preferred embodiment according to the present invention takes for the ease of the completion of manual injector 1 is used alone
Sample process, sample portion 13 and the injection port 23 of chip 2 out of the manual injector 1 are removably tightly connected.It, can be with when sampling
The manual injector 1 is removed, enter sample by the operation control unit 11 and is maintained in the sample cell 12, then
By going out after sample portion 13 connect with the injection port 23 of chip 2 for manual injector 1, operate the control unit 11 will sample described in out
Release the injection port that the sample cell 12 enters the chip 11 in sample portion 13.
It can be to be screwed on or connected by elastic sealing element as the above-mentioned concrete mode being removably tightly connected
It connects, the bottom in the preferably described sample portion 13 out has helicitic texture or flexible seal member, is preferably screwed on.The helicitic texture can
Think internal thread structure or external thread structure；The flexible seal member can be rubber stopper.
Another preferred embodiment according to the present invention, in order to be further simplified device, the manual injector 1
Sample portion 13 is fixedly connected with the injection port 23 of the chip 2 out.It is highly preferred that the sample cell 12 of the manual injector 1, out sample
The injection port 23 of portion 13 and the chip 2 is formed as one, in use, sample can directly be added in sample cell 12, then will
The control unit 11 is coupled with sample cell 12, by operation control portion 11, controls the flow process of sample in the chips.
A further preferred embodiment according to the present invention, the control unit 11 control the inner cavity of sample cell 12
The range of (i.e. the chamber for sample storage of sample cell 12 and the formation of control unit 11) volume change is according in the chip 2
Portion's structure is selected.As long as the sample of specified amount is packed into sample cell as a result, so that it may which accurate control sample is in chip 2
Each runner, the mobility status in reaction tank, facilitate the control that sample flow in experimentation is repeated several times, improve experiment
Repeatability.
In accordance with the present invention it is preferred that the control unit 11 and the sample cell 12 are detachably connected.By by the control
Portion 11 processed and the sample cell 12 are detachably connected, the two can be separated, convenient for adding sample in sample cell.
According to the present invention, there is no particular limitation for the shape of the sample cell 12 and the control unit 11, and the two can phase
Mutually cooperation, realization enter sample and are maintained in the sample cell 12 or sample is made to go out sample portion 13 described in release the sample
Product pond 12.Specifically, the sample cell 12 and the control unit 11 are preferably all cylindricality.The control unit 11 and the sample
Also there is no particular limitation for the connection type in product pond 12, such as can be connected and sealed, be screwed on by seal member etc., from
From the aspect of operating the control unit 11, the preferably described control unit 11 is screwed on the sample cell 12.
A preferred embodiment according to the present invention, the process that the control unit 11 controls sample flow pass through described
The cavity volume of sample cell 12 caused by relative motion between control unit 11 and the sample cell 12, which changes, to be completed.
Preferably, the sample cell 12 and control unit 11 can be set to tube-in-tube structure.Wherein, the control unit 11 can be with
As outer cylinder or inner cylinder.It is highly preferred that the inside of the sample cell 12 is arranged in the control unit 11.Specifically, the sample
Product pond 12 can be the outer cylinder of upper opening, and the control unit 11 can be the inner cylinder of sealed bottom.Preferably, the control unit
11 may be set to be telescopic piston.By setting piston for control unit 11, can be relatively moved in sample cell 12.It is logical
Crossing makes control unit 11 and sample cell 12 cooperate, and control unit 11 relatively moves inside sample cell 12, makes the interior of sample cell 12
Chamber size changes, and control sample enters or outflow sample cell 12.
In order to complete control unit 11 and sample cell 12 and be tightly connected, preferably set in the lateral wall bottom of the control unit 11
It is equipped with sealing ring, the sealing ring is for sealing gap between the control unit 11 and the sample cell 12.
Another preferred embodiment according to the present invention, the process that the control unit 11 controls sample flow pass through institute
The deformation process for stating control unit 11 itself is completed.The control unit 11 have can deformation part, and can deformation part by this
Deformation changes the volume of 12 inner cavity of sample cell.It is described can deformation part shape and set-up mode there is no particular limitation,
Preferably, the control unit 11 can deformations be divided into the scalable side wall of alterable height.It is specifically as follows folding side wall,
For example formed as longitudinal section be continuous N font tubular or middle part can deformation part connection up and down two can not deformations
Point structure (wherein, can deformation part can be made of flexible material, by can deformation part deformation change up and down two not
Can deformation part mutual alignment relation, such as by horizontal connection structure variation be mutual insert structure) etc..The control unit 11
Side wall as can deformations timesharing, preferably deformation occurs at top, and when use can apply pressure or pulling force at the top of this,
Manipulate the Sidewall Height variation of the control unit 11.
As above-mentioned control unit 11 can deformation part material, preferably flexible material, more preferably flexible macromolecule
Polymer is specifically as follows one of polyethylene, polypropylene, polytetrafluoroethylene (PTFE), polytetrafluoroethylene (PTFE) or a variety of, wherein being preferably
Polyethylene and/or polypropylene.
The material of the manual injector 1, it is however preferred to have translucency and chemically inert material.For the ease of observe into
Sample process, the preferably described sample cell 12 are transparent or semitransparent sample cell.As the material of the sample cell 12, preferably high score
Sub- polymer is specifically as follows polyethylene, polypropylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene (PTFE), polycarbonate, poly- methyl
One of methyl acrylate, ABS plastic, phenolic resin, epoxy resin, glass and quartz are a variety of, wherein preferably poly- second
One of alkene, polypropylene, polystyrene and polyvinyl chloride are a variety of.In addition, the control unit 11 is except can be in addition to deformation part
Part and it is described go out sample portion 13 it is also preferred that using above-mentioned material.
In the present invention, which can also include more than one detection part.
According to the present invention, there is no particular limitation for the detection part, can be used for the various detection parts of chip detection,
Such as the detection part can be chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman signal inspection
Survey one of component and infrared spectroscopy detection part or a variety of.It is wherein preferably absorbance detection component and/or fluorescence detection
Component.
For the ease of the progress of detection, the chip can be fixed on the water quality in order to any way of detection joins more
It measures in detection device, such as can fix, rotatable or slidable mode.
A preferred embodiment according to the present invention, the detection part is by light source, branch optical fiber and spectroscopy equipment
It constitutes.The light source is used for for generating the light for carrying out testing required wavelength, the branch optical fiber while detecting multiple reaction tanks
Interior optical signalling, the spectroscopy equipment are used to collect optical signal generating or after absorbing.When needing to different location
When reaction tank is detected, the incident light that the branch optical fiber can will test is divided into multichannel, so that detection be rapidly completed.
According to the present invention, in order to realize that function is integrated, which can also include signal acquisition part
One of part, data processor, data output component and data communication component are a variety of.It is detected as the water quality many reference amounts
A kind of preferred embodiment of equipment comprising data processor, data output component, data transfer component.As described
Data processor can be the central processing unit etc. including controller, arithmetic unit and register；As the data output section
Part may include having display screen, touch screen, voice output of images outputting function etc.；It, can as the data transfer component
It is remotely handled so that initial data is uploaded to remote server, then result is returned directly into designated terminal, can also be incited somebody to action
Data processor generates treated information remote transmitting to cloud and carries out information collection.
Another aspect of the present invention provides a kind of water quality many reference amounts detection method, wherein this method comprises: keeping above-mentioned
In the state of manual injector 1 and chip 2 separation of water quality many reference amounts detection device, the control of the manual injector 1 is operated
Portion 11 enters sample and is maintained in the sample cell 12, and the manual injector 1 is then gone out sample portion 3 and the core
The sealed connection of piece 2, then operate manual injector 1 control unit 11 make sample through it is described go out sample portion 13 release or entered back into it is described
Sample cell 12 to control flowing of the sample in the chip 2, and then completes water quality many reference amounts detection process.By above-mentioned
The cooperation of control unit 11 and sample cell 12, may be implemented the accurate control of 12 cavity volume of sample cell, and sample can according to need
Enter at any time, be maintained at or release in sample cell 12, bring great flexibility, accuracy to array experiment process and can grasp
The property made.
The mode of control unit 11 as manual injector 1 described in aforesaid operations, can be according to the structure of the control unit 11
It is specific to carry out, for example, mode of operation can be to push and pull the control when control unit 11 is piston or has scalable side wall
The top in portion 11；It can certainly be operated with modes such as extruding.Wherein from convenient for operate and control sample move back and forth angle,
The mode of operation preferably pushed and pulled.
In invention, there is no particular limitation for the sample, as long as the operations such as can be reacted or be detected with chip
Fluid sample, such as can be water sample, humoral sample, liquid food etc..
The present invention also provides said chip reaction units or above-mentioned hand sampling method in analytical chemistry, life science
The application in equal fields.
The present invention will be described in detail by way of examples below.
Embodiment 1
Water quality many reference amounts detection device of the invention includes chip reaction unit as shown in Figure 1, the chip reaction unit
Including manual injector 1 and chip 2；
G shows the structure of chip 2 in Fig. 1, and the chip 2 is round dispersion shape multiple flow passages chip, including 1 injection port
23 and 6 reaction tanks being equally spaced with injection port 23,22,6 runners 21 are respectively communicated with each reaction tank 22 and injection port 23；
The injection port 23 of chip 2 is illustrated only in Fig. 1 in a-d, the one group of runner 21 and reaction tank 22 of chip 2 are illustrated only in e-f；
The sample cell 12 of tubular of the manual injector 1 including polyethylene, the control unit 11 of cupuliform and sample portion 13 out,
The inside of the sample cell 12 is arranged in the control unit 11, and is removably tightly connected with the sample cell 12, for making
Sample enters and is maintained in the sample cell 12 or sample is made to release the sample cell 12, and institute through the sample portion 13 out
The lateral wall bottom for stating control 11 is provided with the sealing ring of rubber system, and sample portion 13 is located at the bottom of the sample cell 2 out；
The injection port 23 of the manual injector 1 and the chip 2 is formed as one.
5 μ L fluid samples are added in sample cell 12 first, control unit 11 is placed in sample cell 12, the sealing ring with
The inner sidewall of sample cell 12 contacts, and pushes control unit 11 until touching interface bottom.Sample enters chip 2 under pressure
Runner 21 in, sample flow rate is gradually reduced in flow process, and liquid fills up first reaction tank after 32s, due to this
When 23 end pressure of injection port be less than liquid released into the pressure that reaction tank 22 enters gap between two layers of chip, sample will not continue to
It overflows, all reaction tanks are filled after 19s, and sample stops flowing.
Embodiment 2
Water quality many reference amounts detection device of the invention includes chip reaction unit as shown in Figure 2, the chip reaction unit
Including manual injector 1 and chip 2；
The structure of the chip 2 is in the same manner as in Example 1, and h shows the structure of chip 2 in Fig. 2, illustrates only core in d-e
The injection port 23 of piece 2 illustrates only one group of runner 21 of chip 2 in f-g and reaction tank 22, a-c shows manual injector 1
Sample loading process；
The manual injector 1 includes the tubular sample cell 12 of polyethylene, the tubular control unit 11 being attached thereto and goes out
Sample portion 13, the control unit 11 are connected with the top of the sample cell 12, for entering sample and being maintained at the sample
In pond 12 or sample is made to release the sample cell 12 through the sample portion 13 out, the control unit includes the scalable of alterable height
Side wall as its can deformation part, the shape control of the scalable side wall can be in the range of 0.5-5cm in the height of side wall
It changes, the sample portion 13 out is located at the bottom of the sample cell 2；
The sample portion 13 that goes out of the manual injector 1 is provided with external screw thread, and the injection port 23 of the chip 2 is provided with interior
Screw thread, sample portion 13 is removably screwed on injection port 23 out.
The sample portion 13 that goes out of manual injector 1 is immersed in fluid sample, pulls control by the top for pressing control unit 11 first
Portion 11, a certain amount of sample enter sample cell 12 by going out sample portion 13.Injection port will be screwed in into the sample portion 13 that goes out of manual injector 1
At 23 screw thread, the top of control unit 11 is pressed again, and sample enters under pressure in the runner 21 of chip 2, sample flow
Speed is gradually reduced in flow process, and sample fills up first reaction tank after 12s, and all reaction tanks are filled after 6s, together
When 23 lateral pressure of injection port be gradually decrease to normal pressure, sample stops flowing.
Embodiment 3
Water quality many reference amounts detection device of the invention includes chip reaction unit as shown in Figure 3, the chip reaction unit
Including manual injector 1 and chip 2；
H shows the structure of chip 2 in Fig. 3, and the chip 2 is parallel multi-runner type oblong chips, including three groups according to
Secondary 5 reaction tanks 22 contacted by runner 21, every group of reaction tank are connected to by runner 21 with injection port 23；In Fig. 3 in d-e only
The injection port 23 of chip 2 is shown, the one group of runner 21 and reaction tank 22, a-c that chip 2 is illustrated only in f-g are shown manually
The sample loading process of sample injector 1；
The manual injector 1 includes the tubular sample cell 12 of polyethylene, 11 and of tubular control unit for being connected with piston
Sample portion 13 out, the control unit 11 are connected with the sample cell 12, for entering sample and being maintained at the sample cell 12
In or make sample through it is described go out sample portion 13 release the sample cell 12, the control unit is to be arranged in inside the sample cell
Piston, the sample portion 13 out are located at the bottom of the sample cell 2；
The sample portion 13 that goes out of the manual injector 1 is provided with external screw thread, and the injection port 23 of the chip 2 is provided with interior
Screw thread, sample portion 13 is removably screwed on injection port 23 out.
Control unit 11 is advanced into bottom end first, the sample portion 13 that goes out of manual injector 1 is immersed in fluid sample, is slowly taken out
Control unit 11 out, a certain amount of sample enter sample cell 12 by going out sample portion 13.It will be screwed in into the sample portion 13 that goes out of manual injector 1
At the screw thread of injection port 23, Solid rocket engine portion 11 to bottom end, sample enter under pressure in the runner 21 of chip 2 again,
Sample flow rate is gradually reduced in flow process, and sample fills up first reaction tank after 24s, due to sample introduction head side pressure at this time
Power is less than the pressure that sample release reaction tank is entered to gap between two layers of chip, and sample does not continue to overflow, all anti-after 10s
Ying Chi is filled, and sample stops flowing.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention
In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to
Protection scope of the present invention.
Claims (12)
1. a kind of water quality many reference amounts detection device based on chip reaction unit, which is characterized in that water quality many reference amounts detection is set
Standby includes chip reaction unit, which includes manual injector (1) and chip (2)；
The chip (2) includes runner (21), reaction tank (22) and injection port (23), and passing through the runner (21) will be described anti-
Ying Chi (22) is connected to the injection port (23)；
The manual injector (1) includes sample cell (12), control unit (11) and sample portion (13) out, the control unit (11) and institute
It states sample cell (12) to be connected, for entering sample and being maintained in the sample cell (12) or make the sample warp sample out
Portion (13) releases the sample cell (12), it is described go out sample portion (13) be located at the sample cell (2) with the control unit (11) phase
Pair side；
The injection port (23) of the manual injector (1) and the chip (2) is tightly connected.
2. water quality many reference amounts detection device according to claim 1, wherein the manual injector (1) goes out sample portion
(13) it is removably tightly connected with the injection port (23) of chip (2)；Alternatively, the manual injector (1) goes out sample portion (13)
It is fixedly connected with the injection port (23) of the chip (2).
3. water quality many reference amounts detection device according to claim 1, wherein the control unit (11) and the sample cell
(12) detachably connected；
Preferably, the sample cell (12) and the control unit (11) are cylindricality；
Preferably, the control unit (11) is screwed on the sample cell (12).
4. water quality many reference amounts detection device described in any one of -3 according to claim 1, wherein the sample cell (12) and
Control unit (11) is tube-in-tube structure；
Preferably, inside of control unit (11) setting in the sample cell (12)；
Preferably, the lateral wall bottom of the control unit (11) is provided with sealing ring, and the sealing ring is for sealing the control
Gap between portion (11) and the sample cell (12).
5. water quality many reference amounts detection device described in any one of -3 according to claim 1, wherein control unit (11) tool
Have can deformation part, and by this can the deformation of deformation part change the volume of sample cell (12) inner cavity；
Preferably, the control unit (11) can deformations be divided into the scalable side wall of alterable height.
6. water quality many reference amounts detection device described in any one of -3 according to claim 1, wherein the sample cell (12) is
Transparent or semitransparent sample cell.
7. water quality many reference amounts detection device described in any one of -3 according to claim 1, wherein the sample cell (12)
Material be polyethylene, polypropylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene (PTFE), polycarbonate, polymethyl methacrylate,
One of ABS plastic, phenolic resin, epoxy resin, glass and quartz are a variety of；
Preferably, the control unit (11) can deformation part material be polyethylene, polypropylene, polytetrafluoroethylene (PTFE) and polychlorostyrene second
One of alkene is a variety of.
8. water quality many reference amounts detection device according to any one of claims 1-7, wherein sample portion (13) out
Bottom has helicitic texture or flexible seal member；
Preferably, the helicitic texture is internal thread structure or external thread structure；
Preferably, the flexible seal member is rubber stopper.
9. water quality many reference amounts detection device according to any one of claims 1-7, the water quality many reference amounts detection device
It further include more than one detection part；
Preferably, the detection part is chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman letter
One of number detection part and infrared spectroscopy detection part are a variety of.
10. water quality many reference amounts detection device according to any one of claims 1-7, the water quality many reference amounts detection device
It further include the driving part for driving chip rotation, signal acquisition component, data processor, data output component and data transmission
One of component is a variety of.
11. a kind of water quality many reference amounts detection method, which is characterized in that this method comprises: keeping any one of claim 1-9
In the state of manual injector (1) and chip (2) separation of the water quality many reference amounts detection device, manual injector is operated
(1) control unit (11) enters sample and is maintained in the sample cell (12), then by the manual injector (1)
The sealed connection of sample portion (3) and the chip (2) out, then operate manual injector (1) control unit (11) make sample pass through described in
Sample portion (13) are released or enter back into the sample cell (12) out, to control flowing of the sample in the chip (2).
12. according to claim 1 described in water quality many reference amounts detection device or claim 11 described in any one of -10
Application of the water quality many reference amounts detection method in analytical chemistry or life science.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810449279.XA CN110470601A (en) | 2018-05-11 | 2018-05-11 | Water quality many reference amounts detection device and detection method based on chip reaction unit |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810449279.XA CN110470601A (en) | 2018-05-11 | 2018-05-11 | Water quality many reference amounts detection device and detection method based on chip reaction unit |
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