CN110464868A - A kind of hemostatic material and preparation method thereof that silicate clay is modified - Google Patents
A kind of hemostatic material and preparation method thereof that silicate clay is modified Download PDFInfo
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- CN110464868A CN110464868A CN201910921192.2A CN201910921192A CN110464868A CN 110464868 A CN110464868 A CN 110464868A CN 201910921192 A CN201910921192 A CN 201910921192A CN 110464868 A CN110464868 A CN 110464868A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/06—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention provides a kind of modified hemostatic materials and preparation method thereof of silicate clay, and the hemostatic material is combined by silicate clay and high polymer through electrostatic spinning；The mass ratio of the silicate clay and high polymer is 0.5~2.5:1；The silicate clay includes kaolinite, galapectite, recessed native stick stone or montmorillonite；Wherein, the part silicone hydroxyl of silicate clay and the Hydrogenbond of high polymer, remainder silicone hydroxyl play hemostasis and hydrophilic interaction on hemostatic material surface.Method includes: silicate clay first to be carried out pretreatment and ultrasonication, then be mixed to get electrostatic spinning liquid with high polymer ethanol solution, finally carries out electrostatic spinning, obtains the modified hemostatic material of silicate clay.Gained hemostatic material has many advantages, such as that anthemorrhagic speed is fast, easy to use, be conducive to wound healing, be good biocompatibility, low in cost.
Description
Technical field
The present invention relates to biomedical material technology, in particular to the modified hemostatic material of a kind of silicate clay and
Preparation method.
Background technique
The research and application of novel hemostatic material are the important topics in global medicine and biomaterial scientific domain.No matter
It is that clinical surgery operation or various sudden accidents cause human body to bleed profusely, efficient hemostatic material is for saving life
It is of great significance.The common hemostatic material of Present clinical has oxycellulose class, gelfoam, Fibrin Glue etc. not of the same race
Class.Haemostatic effect difference is very big between these common hemostatic materials, and application conditions are different, cannot fully meet all emergency situations
Demand.
Silicate clay mineral are widely distributed in nature, many kinds of, are a kind of natural resources of preciousness.Silicate
The particle of clay mineral is thin, plasticity is strong, associativity is good, and large specific surface area, and elecrtonegativity is had on particle, there is good object
Adsorptivity and chemical mobility of the surface are managed, and has the advantages that safe and nontoxic.Silicate clay mineral have been found in medical domain
With preferable coagulation function, alternative absorbs the water in blood, and blood coagulation activity substance is effectively concentrated；Make platelet aggregation
Adherency, while coagulation factor can be excited, start intrinsic coagulation pathway, reaches blood coagulation purpose.
Chinese patent CN106039383A discloses a kind of compound Radix Notoginseng white bole bamboo fibre hemostatic gauze and its preparation side
Method, which includes primary or the substrate of regenerated bamboo fiber material, be incorporated on the substrate hemostatic material, for will
The hemostatic material is bonded to the adhesive of the substrate, hemostatic material include kaolin or kaolinite hemostasis clay material and
Pseudo-ginseng hemostatic powder.Above-mentioned hemostatic gauze has the following problems: the adhesive capacity of hemostatic material is limited, and it is easy to adhere to particle on base material
It falls off, these will lead to the reduction of its hemostatic capability and the difficulty of subsequent processing.
It is therefore desirable to provide, a kind of anthemorrhagic speed is fast and easy to use, low-cost hemostatic material.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of modified hemostatic material of silicate clay and its preparation sides
Method makes its purpose is to which silicate clay and high polymer to be combined into the hemostatic material of stable structure with electrostatic spinning technique
The hemostatic material has the advantages that anthemorrhagic speed is fast, easy to use, low in cost.
In order to achieve the above object, the invention provides the following technical scheme:
A kind of hemostatic material that silicate clay is modified, the hemostatic material is by silicate clay and high polymer through Static Spinning
Silk is combined；The silicate clay and high polymer mass ratio are 0.5~2.5:1；The silicate clay include kaolinite,
Galapectite, recessed native stick stone or montmorillonite；Wherein, the part silicone hydroxyl of silicate clay and the Hydrogenbond of high polymer, remainder
Silicone hydroxyl is divided to play hemostasis and hydrophilic interaction on hemostatic material surface；The high polymer include polyvinylpyrrolidone, polyethylene,
L-lactic acid, polyethyl acrylate or polyvinyl alcohol.
Preferably, the silicate clay is galapectite, and the galapectite is distributed galapectite nano particle, described to stop
Blood material be several tow groups at 3D reticular structure, the fibre bundle by nanometer galapectite pass through electrostatic interaction and high polymer
It is compounded to form.
Preferably, the silicate clay is recessed native stick stone, and the attapulgite is distributed palygorskite nano particle,
The hemostatic material is compounded to form by distributed palygorskite nano particle by electrostatic interaction and high polymer.
The present invention also provides a kind of preparation methods of hemostatic material that above-mentioned silicate clay is modified, include the following steps:
(1) silicate clay is subjected to pretreatment and ultrasonication；
(2) high polymer is dissolved in ethyl alcohol, it is 0.1~0.3g/mL high polymer ethanol solution that concentration, which is made,；
(3) step (1) treated silicate clay is mixed with high polymer ethanol solution obtained by step (2), is obtained quiet
Electrospun liquid；
Wherein, the mass ratio of silicate clay and high polymer is 0.5~2.5:1；
(4) electrostatic spinning liquid obtained by step (3) is subjected to electrostatic spinning, obtains the modified hemostatic material of silicate clay；
Wherein, the silicate clay includes kaolinite, galapectite, recessed native stick stone or montmorillonite；The high polymer includes
Polyvinylpyrrolidone, polyethylene, l-lactic acid, polyethyl acrylate or polyvinyl alcohol；
The parameter of electrostatic spinning are as follows: drum rotation speed is 450~500r/min；Receiving distance is 17~21cm；Cathode voltage
For 10~12kV；Cathode voltage is 2~3kV.
Preferably, when the silicate clay is galapectite, recessed native stick stone or montmorillonite, the pretreatment include grinding,
Washing, centrifugal treating.
Preferably, when the silicate clay is kaolinite, the pretreatment includes grinding, washing, centrifugation and pillared place
Reason.
Preferably, pillared liquid used in the pillared processing is dimethyl sulphoxide aqueous solution.
Preferably, the ultrasonication power is 400~800W, and the time is 100~150min.
Preferably, particle mean size of the silicate clay after ultrasonication is less than 1 μm.
Preferably, the liquid speed degree that goes out of electrostatic spinning is 0.05mL/min.
When silicate clay is galapectite: the halloysite nanotubes obtained by pretreatment are due to a large amount of silicone hydroxyls
With very strong agglomeration, the form and high polymer that can be rolled into a ball at nanotube are be combined with each other by hydrogen bond, are formed by electrostatic spinning
Without several bundle weaves at 3D reticulated film.
In the modified hemostatic material of galapectite, halloysite nanotubes form a certain size galapectite group, due to silicon hydroxyl
The effect of base has certain attraction to high polymer, so that the fibre bundle that high polymer is formed attenuates, monolithic film membrane cellular structure
Become more.In hemostasis, on the one hand thinner fibre bundle provides more spaces haemocyte is allowed to enter, thus more fully with
Galapectite contact, the galapectite on the other hand reunited show stronger galapectite haemostatic properties, can activate coagulation factors XII
And blood platelet is activated to accelerate hemostasis.Film entirety hydrophily becomes strong and is more conducive to quickly absorb the moisture content in blood simultaneously, makes blood
Retrogradation improves hematocrite concentration and limit blood flowing, finally achieves hemostasis purpose.
High polymer has the heat-shrinkable of high molecular material due to itself, causes it can be continuous under normal room temperature environment
Contraction, eventually lead to it and only retain 10% or so of original area.And a large amount of ducts between fibre bundle also because shrink and
It reduces or disappears and further restrict the anthemorrhagic performance of film.After galapectite is added, due to the silicone hydroxyl and Polymers Phase of galapectite
In conjunction with, it is suppressed that the overall shrinkage of high polymer retains 70% or more of former film size.Cause simultaneously small-scale in film
The phenomenon that the high-polymer molecule is drawn close to galapectite group, so that the fibre bundle that high polymer is formed attenuates, monolithic film membrane cellular structure becomes
It is more.
When silicate clay is concave convex rod: the palygorskite nano stick pre-processed is due to its stick crystalline substance beam liberation characteristic
The a large amount of silicone hydroxyls having with itself can be combined with each other by hydrogen bond with high polymer, form fiber by electrostatic spinning first
The 3D reticulated film that beam is woven into；Then since high polymer itself has the heat-shrinkable of high molecular material, 3D reticulated film is drilled
Become the film that a surface there are a little attapulgite residual particles.
High polymer has the heat-shrinkable of high molecular material due to itself, causes it can be continuous under normal room temperature environment
Contraction, eventually lead to it and only retain 10% or so of original area.And a large amount of ducts between fibre bundle also because shrink and
It reduces or disappears and further restrict the anthemorrhagic performance of film.And recessed native stick stone hemostasis film thickness is formed, the attapulgite of part dissociation
In the film, support frame structure stablizes overall structure for fusion, and high polymer is inhibited further to shrink, and can retain former thin
70% or more of membrane area.
On the single fiber beam surface of film, remaining recessed native stick stone particle serves as functional particles, improves film table
The hydrophily in face, oxygen-octahedron structure therein have certain hemostatic capability, and blood platelet can be activated to accelerate hemostasis.Only
It can be bonded wound during blood, absorb blood plasma cohesion haemocyte and accelerate hemostasis.
Above scheme of the invention have it is following the utility model has the advantages that
(1) silicate clay that the present invention will be provided with hemostatic function for the first time is made a kind of new in conjunction with electrostatic spinning technique
Type film hemostatic material, is bonded on substrate compared to bleeding-stopping dressing powder or by hemostatic material, and silicate clay is modified to be stopped
Blood material is easy to use when to wound hemostasis, and the water imbibition of material itself and caking property cause it that can be bonded more with the surface of a wound
Closely.
(2) the part silicone hydroxyl of silicate clay forms rock-steady structure with the Hydrogenbond of high polymer in hemostatic material；
Remainder silicone hydroxyl plays hemostasis and hydrophilic interaction on the surface of the material, and obtained hemostatic material has performance stabilization, hemostasis speed
Spend it is fast, easy to use, be conducive to wound healing, good biocompatibility, advantage low in cost.
(3) Polymer Structure that silicate clay is not added is unstable, is easy to that polycondensation occurs, cellular structure is caused to disappear
It loses, specific surface area reduces, and structural advantage brought by electrostatic spinning process is destroyed, and haemostatic effect is bad (comparative example 1)；And it passes through
Silicate clay is modified, and the overall shrinkage of high polymer is inhibited, and silicate clay provides for composite material overall structure
Support, reduces its shrinkage phenomenon, so that the cellular structure of composite material is become more, haemostatic effect is improved (Examples 1 to 3).
Detailed description of the invention
Fig. 1 is the SEM figure for the hemostatic material that the galapectite that the embodiment of the present invention 1 obtains is modified；
Fig. 2 is the SEM figure for the hemostatic material that the attapulgite that the embodiment of the present invention 3 obtains is modified；
Fig. 3 is the SEM figure of the polyethylene pyrrole network alkanone electrostatic spinning film of comparative example 1 of the present invention.
Specific embodiment
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with specific implementation
Example is described in detail.
Embodiment 1
(1) preparation of halloysite nanotubes:
Raw ore galapectite of the 5.0g after grinding, washing and centrifugation is weighed, 500mL deionized water is added in 1500W power
Lower ultrasonication 2h is to get galapectite nanometer rods, and average-size is at 1 μm or less.
(2) preparation of the modified hemostatic material of galapectite:
The polyethylene dissolving pyrrolidones 1.0g into 10mL dehydrated alcohol, is added galapectite nanometer rods 1.50g, and room temperature is lauched
Bath stirring 36h；Carrying out electrostatic spinning setting drum rotation speed is 500r/min, and distance (receive distance) of the syringe needle away from roller is
21cm, liquid speed degree is 0.05mL/min, cathode voltage 10kV, cathode voltage 2kV out, and the modified hemostatic material of galapectite is made
Expect, galapectite group is distributed in galapectite and polyvinylpyrrolidine in the SEM spectrum hemostatic material that galapectite is modified as shown in Figure 1:
Ketone bundle of composite fibers overlaps in the 3D reticular structure to be formed, and the galapectite group is the quiet of itself silicone hydroxyl of galapectite crystal generation
Electro ultrafiltration attracts each other to be formed, and the fibre bundle passes through electrostatic interaction and polyvinylpyrrolidone complex with nanometer galapectite
It is compound by electrostatic interaction and polyvinylpyrrolidine at, galapectite group and then be connected with fibre bundle；The nanometer galapectite
For distributed galapectite nano particle.
(3) hemostasis experiment:
Rat is fixed with 10% chloraldurate (0.3ml/100g) intraperitoneal injection anesthesia, dorsal position, is sterilized, is being taken abdomen just
Middle notch is about 5cm, successively cuts off skin, muscle and peritonaeum, exposure liver.By liver lobus dexter sufficiently expose after its center with
Sharp knife draws the square boundary for taking 1 × 1cm, deep about 0.5cm.Hepatic tissue in boundary is removed with tissue shear, and is scraped with tissue clamps
Except the surface of a wound, the liver surface of a wound is made obvious oozing of blood occur.Hemostatic material made from embodiment 1 is placed in the surface of a wound, sufficiently covers and attaches
Hepatic tissue, with hospital gauze, appropriate compressing and timing above the outer material side surface of a wound, the record complete dwell time of oozing of blood are 120
Second.
Embodiment 2
(1) preparation of halloysite nanotubes:
Raw ore galapectite of the 5.0g after grinding, washing and centrifugation is weighed, 500mL deionized water is added in 1500W power
Lower ultrasonication 2h is to get galapectite nanometer rods, and average-size is at 1 μm or less.
(2) preparation of the modified hemostatic material of galapectite:
The polyethylene dissolving pyrrolidones 1.0g into 10mL dehydrated alcohol, is added galapectite nanometer rods 0.50g, and room temperature is lauched
Bath stirring 36h；Carrying out electrostatic spinning setting drum rotation speed is 500r/min, and distance of the syringe needle away from roller is 21cm, out liquid speed degree
For 0.05mL/min, cathode voltage 10kV, cathode voltage 2kV, the modified hemostatic material of galapectite is made.
(3) hemostasis experiment:
Rat is fixed with 10% chloraldurate (0.3ml/100g) intraperitoneal injection anesthesia, dorsal position, is sterilized, is being taken abdomen just
Middle notch is about 5cm, successively cuts off skin, muscle and peritonaeum, exposure liver.By liver lobus dexter sufficiently expose after its center with
Sharp knife draws the square boundary for taking 1 × 1cm, deep about 0.5cm.Hepatic tissue in boundary is removed with tissue shear, and is scraped with tissue clamps
Except the surface of a wound, the liver surface of a wound is made obvious oozing of blood occur.Hemostatic material made from embodiment 1 is placed in the surface of a wound, sufficiently covers and attaches
Hepatic tissue, with hospital gauze, appropriate compressing and timing above the outer material side surface of a wound, the record complete dwell time of oozing of blood are 125
Second.
Embodiment 3
(1) preparation of palygorskite nano stick:
Raw ore attapulgite of the 5.0g after grinding, washing and centrifugation is weighed, 500mL deionized water is added in 1500W function
Ultrasonication 2h is under rate to get palygorskite nano stick, and average length is at 2 μm or less.
(2) preparation of the modified hemostatic material of concave convex rod:
Palygorskite nano stick 1.50g is added, under room temperature in the polyethylene dissolving pyrrolidones 1.0g into 10mL dehydrated alcohol
Stirring in water bath 36h；Carrying out electrostatic spinning setting drum rotation speed is 500r/min, and distance of the syringe needle away from roller is 21cm, out liquid speed
Degree is 0.05mL/min, cathode voltage 10kV, cathode voltage 2kV.The modified hemostatic material of attapulgite, SEM figure is made
Spectrum is as shown in Figure 2: the hemostatic material is rough surface membrane structure, and the film is with the attapulgite after the dissociation of part stick crystalline substance beam
It is used as main film body by the way that electrostatic interaction and polyvinylpyrrolidone are compound, the concave convex rod of film surface part stick crystalline substance beam dissociation is logical
It crosses part silicone hydroxyl to be connected with film, another part is exposed to the shaggy structure of outer formation while playing hemostasis and parent
Water effect；The nano-attapulgite stone is distributed palygorskite nano particle；The attapulgite part stick crystalline substance beam is dissociated into
Pretreatment when by ultrasonication, mill and be freeze-dried there is a phenomenon where.
(3) hemostasis experiment:
Rat is fixed with 10% chloraldurate (0.3ml/100g) intraperitoneal injection anesthesia, dorsal position, is sterilized, is being taken abdomen just
Middle notch is about 5cm, successively cuts off skin, muscle and peritonaeum, exposure liver.By liver lobus dexter sufficiently expose after its center with
Sharp knife draws the square boundary for taking 1 × 1cm, deep about 0.5cm.Hepatic tissue in boundary is removed with tissue shear, and is scraped with tissue clamps
Except the surface of a wound, the liver surface of a wound is made obvious oozing of blood occur.Hemostatic material made from embodiment 2 is placed in the surface of a wound, sufficiently covers and attaches
Hepatic tissue, with hospital gauze, appropriate compressing and timing above the outer material side surface of a wound, the record complete dwell time of oozing of blood are 140
Second.
Embodiment 4
(1) preparation of kaolinite nanometer sheet:
Weigh raw ore kaolinite of the 10.0g after grinding, washing and centrifugation, be added 90mL dimethyl sulfoxide, 10mL go from
Sub- water.Waters stirring 36h obtains the pillared liquid of kaolinite at 70 DEG C.By the ultrasonication under 1500W power of the pillared liquid of kaolinite
2h is to get kaolinite nanometer sheet, and average-size is at 1 μm or less.
(2) preparation of the modified hemostatic material of kaolinite:
The polyethylene dissolving pyrrolidones 1.0g into 10mL dehydrated alcohol, is added kaolinite nanometer sheet 2.40g, and room temperature is lauched
Bath stirring 36h；Carrying out electrostatic spinning setting drum rotation speed is 500r/min, and distance of the syringe needle away from roller is 21cm, out liquid speed degree
For 0.05mL/min, cathode voltage 10kV, cathode voltage 2kV, the hemostatic material of Modification of kaolin is made.
(3) hemostasis experiment:
Rat is fixed with 10% chloraldurate (0.3ml/100g) intraperitoneal injection anesthesia, dorsal position, is sterilized, is being taken abdomen just
Middle notch is about 5cm, successively cuts off skin, muscle and peritonaeum, exposure liver.By liver lobus dexter sufficiently expose after its center with
Sharp knife draws the square boundary for taking 1 × 1cm, deep about 0.5cm.Hepatic tissue in boundary is removed with tissue shear, and is scraped with tissue clamps
Except the surface of a wound, the liver surface of a wound is made obvious oozing of blood occur.Preprepared hemostatic material is placed in the surface of a wound, sufficiently covers and attaches liver
Tissue, with hospital gauze, appropriate compressing and timing above the outer material side surface of a wound, the record complete dwell time of oozing of blood are 100 seconds.
Comparative example 1
(1) prepared by polyethylene pyrrole network alkanone electrostatic spinning film:
The polyethylene dissolving pyrrolidones 1.0g into 10mL dehydrated alcohol, stirring in water bath 36h under room temperature；Carry out electrostatic spinning
Setting drum rotation speed is 500r/min, and distance of the syringe needle away from roller is 21cm, and liquid speed degree is 0.05mL/min out, and cathode voltage is
10kV, cathode voltage 2kV.Galapectite electrostatic spinning film is made, SEM spectrum is as shown in Figure 3: PVP film is shunk as shown in Figure 3
Degree is big, and PVP serious polycondensations cause the duct almost all in film to disappear.
(2) hemostasis experiment:
Rat is fixed with 10% chloraldurate (0.3ml/100g) intraperitoneal injection anesthesia, dorsal position, is sterilized, is being taken abdomen just
Middle notch is about 5cm, successively cuts off skin, muscle and peritonaeum, exposure liver.By liver lobus dexter sufficiently expose after its center with
Sharp knife draws the square boundary for taking 1 × 1cm, deep about 0.5cm.Hepatic tissue in boundary is removed with tissue shear, and is scraped with tissue clamps
Except the surface of a wound, the liver surface of a wound is made obvious oozing of blood occur.Galapectite electrostatic spinning film made from comparative example 1 is placed in the surface of a wound, sufficiently
Hepatic tissue is covered and attaches, appropriate compressing and timing above the outer material side surface of a wound, record oozing of blood stop completely with hospital gauze
Time is 360 seconds.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, without departing from the principles of the present invention, it can also make several improvements and retouch, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of modified hemostatic material of silicate clay, which is characterized in that the hemostatic material is gathered by silicate clay and height
Object is combined through electrostatic spinning；The mass ratio of the silicate clay and high polymer is 0.5~2.5:1；The silicate is glutinous
Soil includes kaolinite, galapectite, recessed native stick stone or montmorillonite；Wherein, the part silicone hydroxyl of silicate clay and the hydrogen of high polymer
Bond is closed, and remainder silicone hydroxyl plays hemostasis and hydrophilic interaction on hemostatic material surface；The high polymer includes polyethylene pyrrole
Pyrrolidone, polyethylene, l-lactic acid, polyethyl acrylate or polyvinyl alcohol.
2. the modified hemostatic material of silicate clay according to claim 1, which is characterized in that the silicate clay is angstrom
Lip river stone, the galapectite are distributed galapectite nano particle, the hemostatic material be several tow groups at the netted knot of 3D
Structure, the fibre bundle are compounded to form by nanometer galapectite by electrostatic interaction and high polymer.
3. the modified hemostatic material of silicate clay according to claim 1, which is characterized in that the silicate clay is recessed
Native stick stone, the attapulgite are distributed palygorskite nano particle, and the hemostatic material is by distributed palygorskite nano
Particle is compounded to form by electrostatic interaction and high polymer.
4. a kind of preparation method of the modified hemostatic material of silicate clay as described in claims 1 to 3 any one, special
Sign is, includes the following steps:
(1) silicate clay is subjected to pretreatment and ultrasonication；
(2) high polymer is dissolved in ethyl alcohol, it is 0.1~0.3g/mL high polymer ethanol solution that concentration, which is made,；
(3) step (1) treated silicate clay is mixed with high polymer ethanol solution obtained by step (2), obtains Static Spinning
Silk liquid；
Wherein, the mass ratio of silicate clay and high polymer is 0.5~2.5:1；
(4) electrostatic spinning liquid obtained by step (3) is subjected to electrostatic spinning, obtains the modified hemostatic material of silicate clay；
Wherein, the silicate clay includes kaolinite, galapectite, recessed native stick stone or montmorillonite；The high polymer includes poly- second
Alkene pyrrolidone, polyethylene, l-lactic acid, polyethyl acrylate or polyvinyl alcohol；
The parameter of electrostatic spinning are as follows: drum rotation speed is 450~500r/min；Receiving distance is 17~21cm；Cathode voltage is 10
~12kV；Cathode voltage is 2~3kV.
5. preparation method according to claim 4, which is characterized in that the silicate clay be galapectite, recessed native stick stone or
When montmorillonite, the pretreatment includes grinding, washing, centrifugal treating.
6. preparation method according to claim 4, which is characterized in that when the silicate clay is kaolinite, the pre- place
Reason includes grinding, washing, centrifugation and pillared processing.
7. preparation method according to claim 6, which is characterized in that pillared liquid used in the pillared processing is dimethyl sulfoxide
Aqueous solution.
8. preparation method according to claim 4, which is characterized in that the ultrasonication power is 400~800W, and the time is
100~150min.
9. preparation method according to claim 4, which is characterized in that particle of the silicate clay after ultrasonication is flat
Equal size is less than 1 μm.
10. preparation method according to claim 4, which is characterized in that the liquid speed degree that goes out of electrostatic spinning is 0.05mL/min.
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| CN112900095A (en) * | 2021-01-29 | 2021-06-04 | 明光市铭垚凹凸棒产业科技有限公司 | Composite gauze and preparation method and application thereof |
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| CN114712549A (en) * | 2022-05-06 | 2022-07-08 | 中南大学 | Montmorillonite-melanin composite hemostatic powder and preparation method and application thereof |
| CN114788890A (en) * | 2022-05-06 | 2022-07-26 | 中南大学 | Attapulgite-melanin hemostatic gel and preparation method and application thereof |
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