CN110456088B - Specific protein analyzer - Google Patents
Specific protein analyzer Download PDFInfo
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- CN110456088B CN110456088B CN201910794639.4A CN201910794639A CN110456088B CN 110456088 B CN110456088 B CN 110456088B CN 201910794639 A CN201910794639 A CN 201910794639A CN 110456088 B CN110456088 B CN 110456088B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
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Abstract
The invention discloses a specific protein analyzer comprising: a frame; the sample feeding assembly is arranged on the frame and used for conveying samples; a transfer assembly disposed on the frame for transferring the liquid; the reagent bin assembly is arranged on the rack and used for storing detection reagents; a reaction plate assembly disposed on the frame for performing a reaction; the detection assembly is arranged in the frame and positioned at the lower side of the reaction disc assembly, and the detection assembly also comprises a dilution assembly; according to the invention, the dilution assembly is additionally arranged, the blood is pre-diluted through the dilution assembly, the reaction disk assembly is not required to be provided with the dilution cups, the number of the reaction cups on the reaction disk assembly is correspondingly increased, the number of detection items is increased, and meanwhile, the detection rate is improved.
Description
Technical Field
The invention relates to the field of specific protein detection, in particular to a specific protein analyzer.
Background
The specific protein analyzer is mainly used for detecting various proteins in a sample, has very important significance for clinical diagnosis of doctors, and has high accuracy and good repeatability, and can detect a single sample or a large number of samples at any time and immediately output a result.
The traditional full-automatic detecting instrument is operated according to certain time logic requirements in sampling, liquid adding, cleaning, stirring and detecting. As shown in fig. 1, a sample loading position 01, a reagent loading position 02, a stirring position 03, a photoelectric detection position 04 and a cleaning position 05 are respectively arranged on the reaction disk, a certain position interval is provided, and each position is provided with a movable arm assembly for implementing corresponding operation on the corresponding position. Namely, a movable arm is arranged above the reagent sample adding position 02 and connected with a sample adding needle for sample adding, a movable arm is arranged above the stirring position 03 and connected with a stirring rod for stirring liquid, and a movable arm is arranged on the cleaning position 05 and connected with a cleaning pipe for cleaning and the like. In the detection process, the liquid needs to be treated according to a certain time sequence, and the treatment process of one sample is as follows: the empty reaction cup rotates to a reagent sample adding position along with the reaction disc to add a first reagent, then moves to a sample adding position to add a sample, the reaction cup rotates to the reagent sample adding position along with the reaction disc to add a second reagent by a sample adding needle, the reaction cup rotates to a stirring position to be stirred by a stirring rod, the reaction cup rotates to a photoelectric detection position to be detected, a test result is obtained according to detection data, and the reaction cup after detection is cleaned and waits for adding the reagent again.
However, in order to improve the detection accuracy of the raw blood sample, the sample needs to be pre-diluted before the detection. Therefore, in order to adapt to the detection requirement, the existing full-automatic detection instrument is additionally provided with a dilution position on the original reaction disk, the dilution position needs to occupy a reaction cup on the reaction disk, and the pre-dilution action is added in the original working flow of the reaction disk, so that the time logic of the original operation of the reaction disk is disturbed, and the detection speed is further reduced.
Disclosure of Invention
The invention aims to provide a specific protein analyzer, a dilution assembly is additionally arranged, blood is pre-diluted through the dilution assembly, a dilution cup is not required to be placed on a reaction disk assembly, the number of the reaction cups on the reaction disk assembly is correspondingly increased, the number of detected items is increased, and meanwhile, the detection rate is increased.
The aim of the invention can be achieved by the following technical scheme:
a specific protein analyzer comprising: a frame; the sample feeding assembly is arranged on the frame and used for conveying samples; a transfer assembly disposed on the frame for transferring the liquid; the reagent bin assembly is arranged on the rack and used for storing detection reagents; a reaction plate assembly disposed on the frame for performing a reaction; and the detection assembly is arranged in the frame and positioned at the lower side of the reaction disc assembly.
The particular protein analyzer further includes a dilution assembly comprising: a dilution rack; a diluting disc which is rotationally connected to the diluting rack and used for storing diluting liquid and mixing the diluting liquid with a sample, and a plurality of reaction cups are arranged on the diluting disc; a liquid adding component which is arranged on the dilution frame and used for adding the dilution liquid to the dilution disk; and a driving member arranged on the frame for driving the dilution disk to rotate.
Further, the dilution assembly further comprises a dilution mixing device arranged on one side of the dilution disk, the dilution mixing device comprises a mixing piece and a mixing frame fixedly connected to the mixing piece and used for driving the mixing piece to be far away from or close to the reaction cup, and one end of the mixing piece far away from the mixing frame moves along the direction close to the reaction cup and stretches into the reaction cup to enable liquid in the reaction cup to be flushed.
Further, the transfer assembly includes: the sample rocker arm is used for sucking samples into a dilution tray carrying dilution liquid or sucking liquid after the samples are mixed with the dilution liquid into the reaction tray assembly, and the reagent rocker arm is used for sucking reagents in the reagent bin assembly into the reaction tray assembly.
Further, the liquid adding assembly includes: the liquid adding bracket is arranged on the dilution bracket; a plurality of groups of flushing pipes which are fixedly connected to the liquid adding bracket and are arranged in parallel; and the liquid adding pipe is fixedly connected to the liquid adding bracket, and the axis of the liquid adding pipe is parallel to the axis of the flushing pipe.
Further, the diluting disc is in a static state, the mixing piece, the flushing pipe and the liquid adding pipe extend into the diluting disc to respectively stir, flush and add liquid to the reaction cup at the corresponding position, and at the moment, the sample rocker arm moves in the working position along the direction close to the diluting disc to add samples into the reaction cup filled with the diluting liquid.
Further, when the mixing piece, the flushing pipe, the liquid adding pipe and the sample rocker arm move in the direction away from the diluting disc until the reaction cup is pulled out, the diluting disc rotates around the axis of the diluting disc, and the reaction cup carrying the sample and the diluting liquid after the mixing piece is fully mixed is moved to the lower end of the sample rocker arm.
Further, the dilution is rotated around its axis to move the cuvette after the sample swing arm has sucked up the liquid to the lower end of the wash pipe.
Further, the sample rocker arm sucks the liquid obtained by mixing the sample on the dilution disk with the diluent into a reaction cup provided with a reagent on the reaction disk assembly.
Further, the position of the sample rocker arm for adding the sample to the dilution disk is at the same position as the position of the sample rocker arm for sucking the liquid after the sample and the dilution liquid are mixed.
Further, the flushing pipe comprises a liquid inlet pipe and a liquid suction pipe, and one end of the liquid suction pipe, which is close to the dilution disk, is lower than one end of the liquid inlet pipe, which is close to the dilution disk.
The invention has the beneficial effects that:
1. according to the invention, the diluting component is additionally arranged, the diluting component is used for pre-diluting blood, the diluting cup does not need to be placed on the reaction disk component, the number of the reaction cups on the reaction disk component is correspondingly increased, the number of detected items is increased, and meanwhile, the detection rate is increased;
2. the dilution assembly comprises a dilution and mixing device, and the mixed sample and the mixed diluent are subjected to liquid billowing through the dilution and mixing device, so that the sample and the diluent are mixed more thoroughly;
3. the diluting component comprises a liquid adding component, and the diluting disk is timely cleaned and supplemented with diluting liquid through the liquid adding component, so that the reaction cup on the diluting disk can be reused;
4. when the dilution disk is in a static state, the sample rocker arm adds samples, the mixing piece stirs the samples and the dilution liquid, the washing pipe reaction cup is used for washing, and the liquid adding pipe is used for adding the dilution liquid to the washed reaction cup, so that the dilution efficiency of the dilution assembly on the samples is improved;
5. according to the invention, the sample adding to the diluting assembly and the diluted sample adding to the reaction disc are completed by the same sample rocker arm, so that the structure of the invention is more compact.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram of a reaction plate of a conventional protein analyzer;
FIG. 2 is a schematic diagram of a specific protein analyzer according to the present invention;
FIG. 3 is a schematic view of a sample rocker arm structure of the present invention;
FIG. 4 is a schematic view of the dilution assembly of the invention;
fig. 5 is a schematic structural view of the dilution and mixing apparatus of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "open," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like indicate orientation or positional relationships, merely for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the components or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
As shown in fig. 2, a specific protein analyzer includes: a frame 1; the sample feeding assembly is arranged on the frame 1 and used for conveying samples; a transfer assembly 2 provided on the frame 1 for transferring a liquid; a reagent bin assembly 3 arranged on the frame 1 and used for storing detection reagents; a reaction plate assembly 4 provided on the frame 1 for performing a reaction; and a detection assembly 5 arranged in the frame 1 and positioned at the lower end of the reaction disk assembly 4.
The particular protein analyzer also includes a dilution assembly 8 that performs pre-dilution of the sample.
In the prior art, the dilution process is completed on the reaction disk assembly 4, a sample is firstly extracted to one reaction cup in the reaction disk assembly 4 for dilution, then the sample is extracted from the diluted sample cup to the reaction cup, then a detection reagent is added into the reaction cup, and after the reaction is completed, relevant detection is performed and a detection result is output. Thus, the dilution cup will occupy one position on the reaction plate assembly 4, the same test body will need to occupy two positions on the reaction plate assembly 4, and the positions on the reaction plate assembly 4 are limited, thus allowing fewer items to be tested on one test plate and a lower test rate.
According to the invention, the dilution assembly 8 is additionally arranged, the sample is pre-diluted through the dilution assembly 8, the reaction disk assembly 4 does not need to be provided with dilution cups, the number of the reaction cups on the reaction disk assembly 4 is correspondingly increased, the number of detection items is increased, and meanwhile, the detection rate is increased.
The transfer assembly 2 comprises: sample rocker arm 6 and reagent rocker arm 7, sample rocker arm 6 and reagent rocker arm 7 are all provided on frame 1, while sample rocker arm 6 and reagent rocker arm 7 are identical in structure in the embodiment of the invention.
As shown in fig. 3, the sample rocker arm 6 includes: a puncture needle holder 61 provided on the housing 1; a puncture needle supporting frame 62 with a puncture needle arranged at the lower side of one end; the supporting rod 63 is arranged at one end of the puncture needle supporting frame 62 far away from the puncture needle, and the supporting rod 63 is movably assembled on the puncture needle fixing seat 61; the puncture needle moving mechanism 64 fixed on the puncture needle fixing seat 61 and used for driving the supporting rod 63 to move up and down; and a rotary motion mechanism 65 fixed to the puncture needle holder 61 and for driving the support rod 63 to rotate about its axis.
The sample rocker arm 6 further includes: the reinforced linear guide 66, the reinforced linear guide 66 comprises a first guide rod 661 and a first slider 662, the first guide rod 661 is movably assembled with the first slider 662, the first slider 662 slides along the axial direction of the first guide rod 661, the first slider 662 is fixedly connected to the rotary motion mechanism 65, the first guide rod 661 is fixedly connected to the puncture needle support frame 62, and the first guide rod 661 is arranged in parallel with the support rod 63.
In the invention, the reinforced linear guide rail 66 is additionally arranged, so that the lateral torsion of the support rod 63, which is offset from the axis, is reduced, the friction force between the support rod 63 and the puncture needle fixing seat 61 is further reduced, the abrasion of the contact part between the support rod 63 and the fixing seat is reduced, and the normal operation of the support rod 63 is ensured.
The puncture needle moving mechanism 64 comprises a puncture needle synchronous belt mechanism 641 fixed on the puncture needle fixing seat 61, an up-and-down movement supporting block 642 fixedly arranged on a synchronous belt of the puncture needle synchronous belt mechanism 641, and a rotating block 643 fixedly arranged on the up-and-down movement supporting block 642, wherein the rotating block 643 is rotationally connected with the lower end of the supporting rod 63, and a limit boss is arranged at the position of the supporting rod 63 close to the rotating block 643.
When in use, the puncture needle synchronous belt mechanism 641 is started, the synchronous belt of the puncture needle synchronous belt mechanism 641 drives the up-and-down motion supporting block 642 to move along the up-and-down motion direction, and further, the up-and-down motion supporting block 642 drives the supporting rod 63 to move up and down through the rotating block 643, so that the puncture needle on the puncture needle supporting frame 62 moves up and down.
The rotary motion mechanism 65 includes: a puncture needle rotation timing belt mechanism 651 fixed to the puncture needle holder 61; a lower rotary seat 652 fixedly connected with the synchronous wheel of the puncture needle rotary synchronous belt mechanism 651, the synchronous wheel of the puncture needle rotary synchronous belt mechanism 651 fixedly connected with the lower rotary seat 652 is coaxial with the supporting rod 63; the vertical linear guide rail 653 is fixed at the upper end of the lower end rotating seat 652, the vertical linear guide rail 653 comprises a second guide rod 6531 and a second slider 6532, the second guide rod 6531 is fixedly connected with the lower end rotating seat 652, the second guide rod 6531 is movably assembled with the second slider 6532, the second slider 6532 slides along the axial direction of the second guide rod 6531, and the second guide rod 6531 is arranged in parallel with the support rod 63; and a clamping block 654 fixedly connected to the second slider 6532, wherein one end of the clamping block 654 away from the second slider 6532 is clamped and fixed with the support rod 63.
Wherein, the axis of the first guide rod 661 and the axis of the second guide rod 6531 are both positioned between the axis of the supporting rod 63 and the axis of the puncture needle.
Wherein, one end of the first guide rod 661 far away from the puncture needle supporting frame 62 is fixedly connected with the clamping block 654, and the first slider 662 is fixedly connected with the upper end of the second guide rod 6531.
Wherein, the puncture needle rotation synchronous belt mechanism 651 is positioned at the lower end of the puncture needle fixing seat 61.
When the puncture needle rotating synchronous belt mechanism 651 is started in use, the synchronous wheel of the puncture needle rotating synchronous belt mechanism 651 drives the supporting rod 63 to rotate around the axis of the puncture needle through the lower end rotating seat 652, the vertical linear guide rail 653 and the clamping block 654, so that the puncture needle on the puncture needle supporting frame 62 rotates around the axis of the supporting rod 63, and the clamping block 654 slides up and down along the second guide rod 6531 of the vertical linear guide rail 653 when the supporting rod 63 moves up and down; the puncture needle rotating synchronous belt mechanism 651 is positioned at the lower end of the puncture needle fixing seat 61, so that the space layout of the upper end of the puncture needle fixing seat 61 is reduced, and the upper end of the puncture needle fixing seat 61 is more compact.
The steering connecting assembly (corresponding to the vertical linear guide 653 and the clamping block 654) in the prior art is designed at the bottom, and the disadvantage of the design at the bottom is that the overall volume of the bottom is larger, while the steering connecting assembly in the prior art is designed at the middle, so that the space at the upper part of the invention is fully utilized, and the overall volume of the bottom is reduced.
The puncture needle fixing seat 61 is fixedly connected with a safety rod 67, the safety rod 67 is arranged in parallel with the supporting rod 63, the safety rod 67 penetrates through the up-down movement supporting block 642, and the lower end part of the safety rod 67 is sleeved with a supporting spring 68.
When the motor locks and loosens the puncture needle synchronous belt mechanism 641, the puncture needle support frame 62 moves downwards due to self gravity, when the puncture needle is positioned right above a reaction cup on the reaction disc assembly 4 or the dilution assembly 8, the puncture needle on the puncture needle support frame 62 is inserted into the reaction cup, the reaction cup on the reaction disc assembly 4 or the dilution assembly 8 which is prevented from rotating, the puncture needle, the reaction disc assembly 4 or the reaction cup on the dilution assembly 8 is damaged, and the puncture needle support frame 62 is supported by the locking force of the motor through the supporting spring 68, so that the puncture needle on the puncture needle support frame 62 is positioned at a certain height when the motor can not lock the puncture needle synchronous belt mechanism 641, and the puncture needle on the puncture needle support frame is prevented from being inserted into the reaction cup.
During the use, the sample injection subassembly removes the sample pipe in the sample box one by one to sample rocking arm 6 under in proper order, and the pjncture needle pump control puncture is aimed at the sample in the sample pipe and is absorbed, through pjncture needle motion mechanism 64 and rotary motion mechanism 65, makes pjncture needle upper and lower and around bracing piece 63 axis rotary motion, realizes pjncture needle on sample rocking arm 6 and draws the sample in the sample pipe to diluting subassembly 8 in, and pjncture needle pump control puncture is aimed at the sample in the sample pipe and releases, perhaps pjncture needle on sample rocking arm 6 absorbs the sample after diluting subassembly 8 and releases in reaction disk subassembly 4.
As shown in fig. 4, the dilution unit 8 includes: a dilution rack 81 provided on the frame 1; a dilution disk 82 rotatably connected to the dilution frame 81 for storing a dilution liquid and mixing the dilution liquid with a sample, and a plurality of reaction cups are provided on the dilution disk 82; a liquid adding unit 83 provided on the dilution frame 81 for adding a diluent to the dilution tray 82; and a driving member provided on the chassis 1 for driving the dilution disk 82 to rotate.
Wherein, add liquid subassembly 83 includes: a liquid adding stand 831 provided on the dilution frame 81 and moving up and down; a plurality of groups of flushing pipes 832 fixedly connected to the liquid adding support 831 and arranged in parallel; and a liquid adding pipe 833 fixedly connected to the liquid adding bracket 831, the axis of the liquid adding pipe 833 being parallel to the axis of the flushing pipe 832.
The rinse tube 832 includes a feed tube and a draw tube, with the draw tube being positioned at a lower end of the dilution disk 82 than the feed tube.
When the device is used, the liquid inlet pipe pump injects cleaning liquid into the reaction cup of the dilution disk 82 after the sample is extracted from the sample rocker arm 6 through the liquid inlet pipe, the liquid extracting pipe pump extracts polluted cleaning liquid through the liquid extracting pipe, and the flushing pipes 832 are arranged in a plurality of groups, so that the reaction cup of the dilution disk 82 after the sample is extracted from the sample rocker arm 6 can be cleaned for a plurality of times.
In use, the liquid adding tube pump injects the diluent into the reaction cup of the dilution disk 82 after the washing of the washing tube 832 through the liquid adding tube 833, the diluent is typically physiological saline.
The dilution assembly 8 further includes a dilution and mixing apparatus 84 provided on one side of the dilution tray 82, as shown in fig. 5, the dilution and mixing apparatus 84 including a mixing member 841 and a mixing rack.
The mixing piece 841 in the embodiment of the invention is a rotating stirring pin, and when the stirring pin extends into the reaction cup of the dilution disk 82, the stirring pin is driven to rotate by a motor to perform billowing on liquid in the reaction cup of the dilution disk 82.
Wherein, the mixing frame includes: a stirring bracket 842 arranged on the mixing piece; a stirring linear rail 843 that limits movement of the stirring bracket 842; a stirring motion synchronous belt mechanism 844 for driving the stirring bracket 842 to move up and down along the stirring linear guide 843; plastic spline 845 fixedly mounted on stirring bracket 842; a stirring rotation timing belt mechanism 846 for driving the stirring bracket 842 to rotate through the plastic spline 845; and a stirring support seat 847 for supporting and fixing the stirring linear guide 843, the stirring motion synchronous belt mechanism 844 and the stirring rotation synchronous belt mechanism 846, wherein the stirring support 842 is movably mounted with the stirring linear guide 843, the stirring support 842 is synchronously and fixedly connected with the stirring motion synchronous belt mechanism 844, the stirring support seat 847 is fixed at the upper end of the support 1, and the plastic spline 845 penetrates through a synchronous pulley of the stirring rotation synchronous belt mechanism 846.
When the stirring device is used, the plastic spline 845 can move up and down along with the stirring bracket 842, when the synchronous pulley of the puncture needle rotating synchronous belt mechanism 651 rotates, the plastic spline 845 rotates and limits on the synchronous pulley of the puncture needle rotating synchronous belt mechanism 651, at the moment, the synchronous pulley of the puncture needle rotating synchronous belt mechanism 651 rotates to drive the stirring bracket 842 to rotate through the plastic spline 845, and the synchronous belt of the stirring motion synchronous belt mechanism 844 drives the stirring bracket 842 to move up and down along the stirring linear guide rail 843, so that the up-and-down and rotating motion of a mixing piece is realized, and the driving of the mixing piece to be far away from or close to a reaction cup in the diluting disc 82 is further realized.
When the dilution disk 82 is in a static state, the mixing piece 841, the flushing pipe 832 and the liquid adding pipe 833 extend into the dilution disk 82 to respectively stir, flush and add liquid to the reaction cup in the dilution disk 82 at the corresponding position, and at the moment, the sample rocker arm 6 moves along the direction approaching the dilution disk 82 at the working position to add the sample to the reaction cup filled with the diluent;
when the mixing member 841, the flushing pipe 832, the liquid adding pipe 833 and the sample rocker arm 6 move in the direction away from the reaction cup of the dilution disk 82 until the reaction cup is removed, the dilution disk 82 rotates around the axis thereof to move the reaction cup carrying the sample and the diluent after the mixing member 841 is fully mixed to the lower end of the sample rocker arm 6, and the sample rocker arm 6 sucks the liquid after the sample and the diluent on the dilution disk 82 are mixed into the reaction cup containing the reagent on the reaction disk assembly 4.
The position of the sample rocker arm 6 for adding the sample to the reaction cup of the dilution disk 82 is the same as the position of the sample rocker arm 6 for sucking the liquid after the sample of the dilution disk 82 is mixed with the dilution liquid.
The dilution disk 82 rotates around its axis to move the cuvette after the sample swing arm 6 has sucked up the liquid to the lower end of the flushing pipe 832, and the cuvette is flushed.
The reagent bin assembly 3 comprises a reagent bin 31 arranged on the frame 1 and a driving piece for driving the reagent bin 31 to rotate around an axis, a detection reagent groove 311 for placing detection reagents and an R1 reagent groove 312 for placing R1 reagents are axially distributed on the reagent bin 31, the R1 reagents react with samples in the reaction disk assembly 4 in advance to enable the detection effect of the detection reagents to be better, wherein the R1 reagent groove 312 is positioned on the inner side of the detection reagent groove 311, the detection reagents for carrying out turbidity measurement after reacting with the samples on the reaction disk assembly 4 are placed in the detection reagent groove 311, and the R1 reagents for carrying out impurity removal on the samples to be reacted on the reaction disk assembly 4 are placed in the R1 reagent groove 312.
When the reagent needle on the reagent rocker arm 7 is used, the reagent needle extracts the R1 reagent into the empty reaction cup of the reaction disk assembly 4 in advance, the puncture needle on the sample rocker arm 6 extracts the diluted sample in the dilution assembly 8 into the reaction cup of the reaction disk assembly 4 which is pre-injected with the R1 reagent, at the moment, the R1 reagent reacts with impurities in the sample to remove the impurities, and the reagent needle on the reagent rocker arm 7 extracts the detection reagent into the reaction cup of the reaction disk assembly 4 to remove the impurities and react.
In the prior art, detection reagents and R1 reagents are generally and orderly arranged on one side or two sides of a reagent rocker arm 7 in a rectangular distribution mode, the detection reagents and the R1 reagents are influenced by the movement range of the reagent rocker arm 7, at the moment, the types of the detection reagents and the R1 reagents are limited, and only 3-4 types of detection reagents can be used, so that the number of detected items is limited.
The reaction disk assembly 4 includes a reaction disk 41 mounted on the frame 1 and provided with reaction cups, and a driving member for driving the reaction disk 41 to rotate about its axis.
The reaction turntable 41 is provided with a reaction turntable stirring mechanism 42 for stirring samples and reagents mixed in the reaction turntable 41 and a reaction turntable washing station 43 for washing and drying the reaction turntable 41 after turbidity measurement in sequence on the periphery side, wherein the reaction turntable stirring mechanism 42 and the reaction turntable washing station 43 are fixedly arranged at the upper end of the frame 1, and the reaction turntable stirring mechanism 42 and the reaction turntable washing station 43 are positioned on two sides of the detection assembly 5.
Compared with the dilution and mixing device 84, the reaction turntable stirring mechanism 42 is characterized in that two mixing pieces 841 are arranged on the mixing rack of the reaction turntable stirring mechanism 42, and the reaction turntable stirring mechanism 42 realizes the simultaneous stirring of detection reagents and sample reactants or R1 reagents and sample reactants in adjacent reaction cups on the reaction turntable 41 through the two mixing pieces 841, so that the working efficiency of the whole device is improved.
The reaction turntable washing station 43 includes a washing rack 431 provided on the frame 1; a plurality of groups of flushing pipes 832 fixedly connected to the cleaning support member 431 and arranged in parallel.
When the device is used, the reaction turntable stirring mechanism 42 is used for stirring the reaction liquid of the sample and the R1 reagent in the reaction cup of the reaction turntable 41 firstly, so that the R1 reagent is fully contacted with the sample, the impurity removal speed of the sample in the reaction cup of the reaction turntable 41 is accelerated, meanwhile, the impurity removal speed of the sample is more thoroughly, the reaction liquid of the sample and the detection reagent after impurity removal is stirred by the reaction turntable stirring mechanism 42 secondly, the detection reagent is fully contacted with the sample after impurity removal, the reaction speed of the sample after impurity removal in the color disk is accelerated, meanwhile, the reaction cup filled with the thoroughly reacted sample and the detection reagent reactant is thoroughly reacted, the reaction cup is rotated to the detection component 5 through the reaction turntable 41, the detection component 5 is used for carrying out turbidity measurement on the thoroughly reacted sample and the detection reagent reactant, the reaction turntable 41 is rotated after the turbidity measurement, the reaction cup filled with the sample and the detection reagent reactant is rotated to the position right below the reaction turntable washing station 43, the reaction cup filled with the sample and the detection reagent reactant is removed at the same time, and the removed reaction cup is washed, and the preparation is made for adding the sample next time.
The detection assembly 5 may be a conventional turbidimeter that performs turbidimetry on the reactants in the cuvette rotated to the detection assembly 5.
The driving member may be a common synchronous belt mechanism or a motor for driving the dilution disk 82 and the reagent cartridge 31 to rotate around the axis thereof.
The invention also comprises a cleaning pool arranged under the reaction rotary table stirring mechanism 42 and the dilution and mixing device 84, when in use, the reaction rotary table stirring mechanism 42 and the dilution and mixing device 84 move the mixing piece 841 after the liquid in the reaction cup is billowed into the cleaning pool, the mixing piece 841 is cleaned, and the residual liquid on the mixing piece 841 is prevented from influencing the next detection.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims.
Claims (7)
1. A specific protein analyzer comprising:
a frame (1);
the sample feeding assembly is arranged on the frame (1) and used for conveying samples;
a transfer assembly (2) arranged on the frame (1) for transferring a liquid;
the reagent bin assembly (3) is arranged on the frame (1) and used for storing detection reagents;
a reaction plate assembly (4) arranged on the frame (1) and used for carrying out reaction;
the detection assembly (5) is arranged in the frame (1) and is positioned at the lower end of the reaction disc assembly (4);
characterized in that the specific protein analyzer further comprises a dilution assembly (8), the dilution assembly (8) comprising:
a dilution rack (81);
a dilution disk (82) rotatably connected to the dilution frame (81) and used for storing a dilution liquid and mixing the dilution liquid with a sample, wherein a plurality of reaction cups are arranged on the dilution disk (82);
a liquid adding unit (83) provided on the dilution frame (81) and configured to add a diluent to the dilution tray (82);
and a driving member provided on the chassis (1) for driving the dilution disk (82) to rotate;
the dilution assembly (8) further comprises a dilution mixing device (84) arranged on one side of the dilution disk (82), the dilution mixing device (84) comprises a mixing piece (841) and a mixing frame fixedly connected to the mixing piece (841) and used for driving the mixing piece (841) to be far away from or close to the reaction cup, and one end of the mixing piece (841) far away from the mixing frame moves along the direction of approaching to the reaction cup and stretches into the reaction cup to cause liquid in the reaction cup to surge;
the transfer assembly (2) comprises: a sample rocker arm (6) and a reagent rocker arm (7), wherein the sample rocker arm (6) is used for sucking a sample into a dilution disk (82) loaded with dilution liquid or sucking liquid obtained by mixing the sample with the dilution liquid into the reaction disk assembly (4), and the reagent rocker arm (7) is used for sucking a reagent in the reagent bin assembly (3) into the reaction disk assembly (4);
the liquid adding component (83) comprises:
a liquid adding bracket (831) arranged on the dilution frame (81);
a plurality of groups of flushing pipes (832) which are fixedly connected to the liquid adding bracket (831) and are arranged in parallel;
and a liquid adding pipe (833) fixedly connected to the liquid adding support (831), wherein the axis of the liquid adding pipe (833) is parallel to the axis of the flushing pipe (832).
2. The specific protein analyzer according to claim 1, wherein the dilution disk (82) is in a stationary state, the mixing member (841) and the flushing pipe (832) and the liquid adding pipe (833) extend into the dilution disk (82) to stir, flush and add liquid to the reaction cup at the corresponding position, respectively, and at this time, the sample rocker arm (6) moves in a direction approaching the dilution disk (82) at the working position thereof to add a sample to the reaction cup filled with the diluent.
3. The specific protein analyzer as claimed in claim 2, wherein the mixing member (841), the flushing pipe (832), the liquid adding pipe (833) and the sample rocker arm (6) are moved in a direction away from the dilution tray (82) until the reaction cup is withdrawn, and the dilution tray (82) is rotated about its axis to move the reaction cup carrying the sample and the dilution liquid after the mixing member (841) is sufficiently mixed to the lower end of the sample rocker arm (6).
4. A specific protein analyzer according to claim 3, characterized in that rotation of the dilution disk (82) about its axis moves the cuvette after the sample rocker arm (6) has sucked up the liquid to the lower end of the wash pipe (832).
5. The specific protein analyzer as set forth in claim 4, wherein said sample rocker arm (6) aspirates the mixed sample and diluent fluid on said dilution disk (82) into a reagent-containing cuvette on said reaction disk assembly (4).
6. The specific protein analyzer according to claim 1, characterized in that the sample rocker arm (6) is located at the same position as the sample rocker arm (6) is located at the position where the sample is added to the dilution tray (82) and the sample rocker arm (6) sucks the liquid after the sample is mixed with the dilution liquid.
7. The specific protein analyzer as set forth in claim 1, wherein the wash tube (832) includes a feed tube and a draw tube, the draw tube having an end proximate the dilution tray (82) lower than an end of the feed tube proximate the dilution tray (82).
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| CN112147186B (en) * | 2020-09-23 | 2023-07-18 | 深圳市科曼医疗设备有限公司 | Specific protein analysis method and control device based on blood cell analyzer |
| CN114965886B (en) * | 2022-05-18 | 2023-11-03 | 深圳无疆生命科学有限公司 | Sample loading tray device and sample analyzer |
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