CN110455947A - A kind of device and detection method for Detecting Pesticide - Google Patents
A kind of device and detection method for Detecting Pesticide Download PDFInfo
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- CN110455947A CN110455947A CN201910733560.0A CN201910733560A CN110455947A CN 110455947 A CN110455947 A CN 110455947A CN 201910733560 A CN201910733560 A CN 201910733560A CN 110455947 A CN110455947 A CN 110455947A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The invention discloses a kind of devices and detection method for Detecting Pesticide, including detection case, detector, sampling structure, heating component, temperature-controlling component and electrically-controlled component, the sampling structure is placed in detection case section, heating component is located at sampling structure bottom, temperature-controlling component and heating component and electrically-controlled component are electrically connected, and the detector is located at detection case side.The invention has the advantages that the technical program provides a kind of completely new detection method and detection device, realize that the quick detection to agricultural product prevents unqualified agricultural product from entering market to guarantee the safety of agricultural product by the equipment division.
Description
Technical field
The present invention relates to Detecting Pesticide fields, especially a kind of for the device of Detecting Pesticide and detection side
Method.
Background technique
One, the main forms of pesticide harm to the human body.Three kinds of forms: acute poisoning, chronic hazard, " three cause " danger
Evil.
Acute poisoning pesticide is oral, the road Xi Hu or contact and largely enter in human body, what is shown in a short time is acute
Pathological reaction is acute poisoning.Acute poisoning often leads to neural paralysis or even death, or even causes Large Scale Death, becomes most
Apparent pesticide harm.
Chronic hazard Long Term Contact or the edible food containing pesticide residue, can be such that pesticide constantly accumulates in vivo, to people
Body health constitutes potential threat, can influence nervous system, destroys liver function, causes physiological barrier, influence reproductive system, generates
Lopsided monster, leads to cancer.
Carcinogenic, teratogenesis, mutagenesis international cancer research institution confirm according to zoopery, 18 kinds of widely used pesticides
With apparent carcinogenicity, there are also 16 kinds of potential carcinogenic risks of display.Wherein dibromochloropropane can cause male sterility, right
Animal has carcinogenic, mutagenesis.Plictran, Mortopl pellet have teratogenesis to animal.Bromofume can make people, animal teratogenesis, cause
Mutation.Spanon has potential carcinogenic threat to people, has carcinogenesis to animal.
Two, the potential hazard of main pesticide.
Organophosphorus insecticide can cause nerve dysfunction, tremble, amentia, speech disorders etc. as neurotoxic substance
Performance.
Pyrethroid lipid pesticide, general toxicity is larger, there is accumulation, and poisoning manifestations symptom is neurological symptom and skin
Skin irritation.
The organo-chlorine pesticides such as six six six, DDT.After organo-chlorine pesticide enters human body with food approach, main accumulation is in rouge
It is secondly liver,kidney,spleen, in brain in fat.Fetus, which is transmitted to, by human milk causes next-generation lesion.
Three, the performance and reason of pesticide poisoning.Less serious case shows as headache, giddy, nausea, burnout, abdominal pain etc., and severe one occurs
Spasm, expiratory dyspnea, stupor, gatism, in addition it is dead.
Four, harm of the pesticide to soil
Influence of the pesticide to farmland physicochemical property.Obvious acidification will appear by the agricultural land soil of pesticide long-term pollution；Soil
Nutrient (nitrogen, phosphorus, potassium etc.) is aggravated with pollution level and is reduced；Soil porosity becomes smaller, to cause soil texture hardened.
Five, harm of the pesticide to pregnant woman, fetus.The pollution of pesticide can cause the decline of male fecundity, moreover it is possible to draw
Play cancer and other visceral lesions.Children are in growth and development stage, grow the more adult more static cell of rapid cell more
Influence vulnerable to carcinogenic pesticide.A large amount of statistics show woman gestationperiod exposed to pesticides, miscarriage, premature labor, stillborn foetus and congenital
The incidence of sex deviation obviously increases, and pesticide can make gene normal control process that steering or growth retardation of fetus occur, to make
At the structure or functions such as congenital abnormality, down's syndrome exception.It is serious to stop development of fetus completely, generation miscarriage,
Premature labor or stillborn foetus.
In the production process of the agricultural product such as vegetable melon and fruit, Organophosphorus and carbamate pesticides class pesticide is widely used, this
Two class action of agricultural chemicals mechanism are combined closely by the active part with acetylcholinesterase, are lost it and are decomposed acetylcholine
Ability.Similarly there is toxicity to the mankind while removing pest in Organophosphorus and carbamate pesticides class pesticide, so to agriculture
The remaining monitoring of medicine is increasingly important.
Existing pesticide residue detection method mainly passes through gas phase or liquid chromatography, and the detection of these detection methods is accurate, but
Professional equipment and professional person is needed to operate simultaneously；In terms of Fast Determination of Pesticide Residue, it is typically based on outside acetylcholinesterase
Add substance that show color to chemically react, then determines persticide residue, this survey by comparing shade difference with colorimetric card
Mode is fast and convenient, but due to everyone difference in operation, it is not accurate enough to frequently result in detection.
Therefore, it is necessary to further be developed to existing Detecting Pesticide card and pesticide residue detection method, to keep away
Exempt from drawbacks described above.
Summary of the invention
The purpose of the present invention is to solve the above problem, a kind of device for Detecting Pesticide and inspection are devised
Survey method.
Realize above-mentioned purpose the technical scheme is that, it is a kind of for the device of Detecting Pesticide and detection side
Method, including detection case, detector, sampling structure, heating component, temperature-controlling component and electrically-controlled component, the sampling structure are placed in
Detection case section, heating component is located at sampling structure bottom, temperature-controlling component and heating component and electrically-controlled component is electrically connected, described
Detector is located at detection case side；
Wherein, sampling structure includes: sampling rack, operation panel and lay out board；
Wherein sampling is placed on detection case end face, and operation panel is inlaid on detection case, and lay out board is placed on sampling rack；
Wherein, heating component mainly includes: heating sheet, mounting rack and heated seats；
The heating sheet is inlaid on mounting rack, and the mounting rack is located inside detection case, and heated seats are inlaid in operation panel
On, it is bonded between heating sheet and heated seats；
Temperature-controlling component mainly includes: temperature sensor and pressure sensor；
The temperature sensor and pressure sensor are located in heated seats, and are electrical connected with electrically-controlled component.
One kind being used for pesticide residue detection method, includes the following steps:
A: preparation acetylcholine ester enzymatic reagent is saved reagent accordingly；
B: taking out sample on sampling rack, prepares the surface of different product, takes the inside human body, epidermis and the vegetable of fruit
The blade of dish, is placed on lay out board；
C: selection container is placed in sampling structure in the heated seats on operation panel, and utilizes the heating sheet pair in heating component
Heated seats are heated, and corresponding preparation is placed in container；
D: after completing heating reaction, the liquid in container is collected, and collection liquid is taken using dropping liquid stick, is placed in color
It is detected in spectrometer.
Preferably, the detection case is rectangular box, and internal with cavity body structure.
Preferably, position is bonded between the heating sheet and heated seats equipped with thermally conductive sheet；The thermally conductive sheet is for conducting heat.
Preferably, the electrically-controlled component mainly includes: the time relay, controller and fuse；
The time relay, controller, fuse, temperature sensor, pressure sensor are electrically connected.
Preferably, cold storage mode should be used when the acetylcholinesterase reagent storage.
Preferably, four degrees Celsius should be maintained at when the acetylcholinesterase reagent storage.
Preferably, the thermally conductive sheet uses ceramic-type thermally conductive sheet.
A kind of device and detection method for Detecting Pesticide made using technical solution of the present invention, this skill
Art scheme provides a kind of completely new detection method and detection device, realizes the quick detection to agricultural product by the equipment division,
To guarantee the safety of agricultural product, prevent unqualified agricultural product from entering market, mentioned in effective solution background technique
Existing pesticide residue detection method mainly pass through gas phase or liquid chromatography, the detection of these detection methods precisely, but needs simultaneously
Professional equipment and professional person is wanted to operate；In terms of Fast Determination of Pesticide Residue, it is typically based on the additional colour developing of acetylcholinesterase
Substance chemically reacts, then persticide residue is determined by comparing shade difference with colorimetric card, and this survey mode is fast
It is fast easy, but due to everyone difference in operation, frequently result in and detect not accurate enough technical problem.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of device for Detecting Pesticide of the present invention；
Fig. 2 is a kind of side structure schematic view of device for Detecting Pesticide of the present invention；
In figure, 1, detection case；2, detector；3, sampling rack；4, operation panel；5, lay out board；6, heating sheet；7, mounting rack；
8, heated seats；9, temperature sensor；10, pressure sensor；11, thermally conductive sheet；12, the time relay；13, controller；14, it fuses
Device.
Specific embodiment
The present invention is specifically described with reference to the accompanying drawing, as shown in Figs. 1-2, a kind of dress for Detecting Pesticide
It sets including detection case 1, detector 2, sampling structure, heating component, temperature-controlling component and electrically-controlled component, the sampling structure is placed in
1 section of detection case, heating component is located at sampling structure bottom, temperature-controlling component and heating component and electrically-controlled component is electrically connected, institute
It states detector 2 and is located at 1 side of detection case；
Wherein, sampling structure includes: sampling rack 3, operation panel 4 and lay out board 5；
Wherein sampling rack 3 is placed in 1 end face of detection case, and operation panel 4 is inlaid on detection case 1, and lay out board 5 is placed in sampling rack 3
On；
Wherein, heating component mainly includes: heating sheet 6, mounting rack 7 and heated seats 8；
The heating sheet 6 is inlaid on mounting rack 7, and the mounting rack 7 is located inside detection case 1, and heated seats 8 are inlaid in behaviour
Make on plate 4, is bonded between heating sheet 6 and heated seats 8；
Temperature-controlling component mainly includes: temperature sensor 9 and pressure sensor 10；
The temperature sensor 9 is located in heated seats 8 with pressure sensor 10, and is electrical connected with electrically-controlled component.
One kind being used for pesticide residue detection method, includes the following steps:
A: preparation acetylcholine ester enzymatic reagent is saved reagent accordingly；
B: taking out sample on sampling rack 3, prepares the surface of different product, take the inside human body of fruit, epidermis and
The blade of vegetables is placed on lay out board 5；
C: selection container is placed in the heated seats 8 in sampling structure on operation panel 4, and utilizes the heating sheet in heating component
6 pairs of heated seats 8 heat, and corresponding preparation is placed in container；
D: after completing heating reaction, the liquid in container is collected, and collection liquid is taken using dropping liquid stick, is placed in color
It is detected in spectrometer；
The detection case 1 is rectangular box, and internal with cavity body structure；It is bonded between the heating sheet 6 and heated seats 8
Position is equipped with thermally conductive sheet 11；The thermally conductive sheet 11 is for conducting heat；The electrically-controlled component mainly includes: the time relay 12, control
Device 13 and fuse 14 processed；The time relay 12, controller 13, fuse 14, temperature sensor 9, pressure sensor
10 are electrically connected；Cold storage mode should be used when the acetylcholinesterase reagent storage；The acetylcholine ester enzymatic reagent is deposited
Four degrees Celsius are maintained at when putting；The thermally conductive sheet 11 uses ceramic-type thermally conductive sheet.
Embodiment
In the present embodiment, the main body of this case is by including detection case 1, detector 2, sampling structure, heating component, temperature control group
Part and electrically-controlled component composition, wherein sampling structure is placed in 1 section of detection case, and heating component is located at sampling structure bottom, temperature control
Component and heating component and electrically-controlled component are electrically connected, and detector 2 is located at 1 side of detection case；
Wherein sample is taken by sampling structure, is saved；Sample is reacted with reagent using heating component, heat is provided,
And it is controlled and is protected by temperature-controlling component and electrically-controlled component when heating；
Wherein, sampling structure includes: sampling rack 3, operation panel 4 and lay out board 5；
Wherein sampling rack 3 is placed in 1 end face of detection case, and operation panel 4 is inlaid on detection case 1, and lay out board 5 is placed in sampling rack 3
On；
Wherein, heating component mainly includes: heating sheet 6, mounting rack 7 and heated seats 8；
Heating sheet 6 is inlaid on mounting rack 7, and mounting rack 7 is located inside detection case 1, and heated seats 8 are inlaid on operation panel 4,
It is bonded between heating sheet 6 and heated seats 8；
Temperature-controlling component mainly includes: temperature sensor 9 and pressure sensor 10；
Temperature sensor 9 and pressure sensor 10 are located in heated seats 8, and are electrical connected with electrically-controlled component.
A kind of novel detection device is provided among the above, which can realize the purpose quickly detected, in background technique
In refer to that existing pesticide residue detection method mainly passes through gas phase or liquid chromatography, the detection of these detection methods is precisely, but same
When need professional equipment and professional person to operate；In terms of Fast Determination of Pesticide Residue, it is additional to be typically based on acetylcholinesterase
Substance that show color chemically reacts, then determines persticide residue, this survey side by comparing shade difference with colorimetric card
Formula is fast and convenient, but due to everyone difference in operation, frequently results in and detect not accurate enough technical problem, this case is for above-mentioned
Problem designs a kind of novel detection device, and application method is as follows:
In specific detection, with the following method:
A: preparation acetylcholine ester enzymatic reagent is saved reagent accordingly；
B: taking out sample on sampling rack 3, prepares the surface of different product, take the inside human body of fruit, epidermis and
The blade of vegetables is placed on lay out board 5；
C: selection container is placed in the heated seats 8 in sampling structure on operation panel 4, and utilizes the heating sheet in heating component
6 pairs of heated seats 8 heat, and corresponding preparation is placed in container；
D: after completing heating reaction, the liquid in container is collected, and collection liquid is taken using dropping liquid stick, is placed in color
It is detected in spectrometer.
In specific implementation process, further, detection case 1 is rectangular box, and internal with cavity body structure.
In specific implementation process, further, position is bonded between heating sheet 6 and heated seats 8 equipped with thermally conductive sheet 11；This is led
Backing 11 is for conducting heat.
In specific implementation process, further, electrically-controlled component mainly includes: the time relay 12, controller 13 and molten
Disconnected device 14；
The time relay 12, controller 13, fuse 14, temperature sensor 9, pressure sensor 10 are electrically connected.
In specific implementation process, further, when acetylcholinesterase reagent storage, should use cold storage mode.
In specific implementation process, further, when acetylcholinesterase reagent storage, should be maintained at four degrees Celsius.
In specific implementation process, further, thermally conductive sheet 11 uses ceramic-type thermally conductive sheet.
Above-mentioned technical proposal only embodies the optimal technical scheme of technical solution of the present invention, those skilled in the art
The principle of the present invention is embodied to some variations that some of them part may be made, belongs to the scope of protection of the present invention it
It is interior.
Claims (8)
1. a kind of device for Detecting Pesticide, including detection case (1), detector (2), sampling structure, heating component, control
Warm component and electrically-controlled component, which is characterized in that the sampling structure is placed in detection case (1) section, and heating component is located at sampling
Structural base, temperature-controlling component and heating component and electrically-controlled component are electrically connected, and the detector (2) is located at detection case (1) side；
Wherein, sampling structure includes: sampling rack (3), operation panel (4) and lay out board (5)；
Wherein sampling rack (3) is placed in detection case (1) end face, and operation panel (4) is inlaid on detection case (1), and lay out board (5), which is placed in, to be taken
In sample rack (3)；
Wherein, heating component mainly includes: heating sheet (6), mounting rack (7) and heated seats (8)；
The heating sheet (6) is inlaid on mounting rack (7), and the mounting rack (7) is located at detection case (1) inside, and heated seats (8) are embedding
On operation panel (4), it is bonded between heating sheet (6) and heated seats (8)；
Temperature-controlling component mainly includes: temperature sensor (9) and pressure sensor (10)；
The temperature sensor (9) and pressure sensor (10) are located on heated seats (8), and are electrical connected with electrically-controlled component.
2. according to claim 1 a kind of for pesticide residue detection method, which comprises the steps of:
A: preparation acetylcholine ester enzymatic reagent is saved reagent accordingly；
B: taking out sample on sampling rack (3), prepare the surface of different product, takes the inside human body, epidermis and the vegetable of fruit
The blade of dish is placed on lay out board (5)；
C: selection container is placed in the heated seats (8) on operation panel in sampling structure (4), and utilizes the heating sheet in heating component
(6) heated seats (8) are heated, and corresponding preparation is placed in container；
D: after completing heating reaction, the liquid in container is collected, and collection liquid is taken using dropping liquid stick, is placed in chromatograph
Inside detected.
3. a kind of device for Detecting Pesticide according to claim 1, which is characterized in that the detection case (1)
For rectangular box, and internal there is cavity body structure.
4. a kind of device for Detecting Pesticide according to claim 1, which is characterized in that the heating sheet (6)
Position is bonded between heated seats (8) equipped with thermally conductive sheet (11)；The thermally conductive sheet (11) is for conducting heat.
5. a kind of device for Detecting Pesticide according to claim 1, which is characterized in that the electrically-controlled component master
Include: the time relay (12), controller (13) and fuse (14)；
The time relay (12), controller (13), fuse (14), temperature sensor (9), pressure sensor (10) are electrically
Connection.
6. according to claim 2 a kind of for pesticide residue detection method, which is characterized in that the acetylcholinesterase
Cold storage mode should be used when reagent storage.
7. according to claim 6 a kind of for pesticide residue detection method, which is characterized in that the acetylcholinesterase
Four degrees Celsius should be maintained at when reagent storage.
8. a kind of device for Detecting Pesticide according to claim 4, which is characterized in that the thermally conductive sheet (11)
Using ceramic-type thermally conductive sheet.
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| CN201910733560.0A CN110455947A (en) | 2019-08-09 | 2019-08-09 | A kind of device and detection method for Detecting Pesticide |
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Cited By (1)
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