CN110452819A - A kind of observable hypobaric hypoxia cell incubator - Google Patents
A kind of observable hypobaric hypoxia cell incubator Download PDFInfo
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- CN110452819A CN110452819A CN201910689648.7A CN201910689648A CN110452819A CN 110452819 A CN110452819 A CN 110452819A CN 201910689648 A CN201910689648 A CN 201910689648A CN 110452819 A CN110452819 A CN 110452819A
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- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 24
- 230000007954 hypoxia Effects 0.000 title claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 15
- 239000007789 gas Substances 0.000 claims abstract description 14
- 238000005086 pumping Methods 0.000 claims abstract description 14
- 235000015097 nutrients Nutrition 0.000 claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 30
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 28
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims description 15
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 14
- 239000001569 carbon dioxide Substances 0.000 claims description 14
- 210000004027 cell Anatomy 0.000 claims 11
- 210000003463 organelle Anatomy 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000010261 cell growth Effects 0.000 abstract description 5
- 238000002701 cell growth assay Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000018875 hypoxemia Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000036413 temperature sense Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/54—Constructional details, e.g. recesses, hinges hand portable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a kind of observable hypobaric hypoxia cell incubator, including cabinet body, sealing cover is provided at the top of cabinet body, bottom of cabinet bulk is provided with constant temperature blending instrument, constant temperature blending instrument upper surface is provided with cell vessel, and constant temperature blending instrument runs through bottom, and cell vessel are located in cabinet body；Cabinet body passes through pipeline respectively and connect with vaccum-pumping equipment, gas transportation facilities；Nutrient delivery device and observation device are additionally provided on cabinet body；Observing device includes microscope, microscope liftable.The present invention is by setting gas transportation facilities, temperature inductor, humidity sensor, pressure inductor, constant temperature blending instrument, convenient for carrying out cell experiment under low-oxygen environment.By the way that vaccum-pumping equipment is arranged, it is pressurized and depressurizes convenient for interior of the cabinet；Device is observed by setting, convenient for observing the cell growth condition of interior of the cabinet, to achieve the effect that convenient for cell growth assay under low pressure.
Description
Technical field
The invention belongs to Bioexperiment device technical fields, are related to a kind of observable hypobaric hypoxia cell incubator.
Background technique
Under the conditions of hypobaric hypoxia pathological study in the past mostly using animal carry out required for simulated experiment, the device needed and
Material occupied space is larger, and required device is many and diverse valuable, and control precision is extremely difficult to require.As scientific development is replaced using cell
The hypobaric hypoxia simulated experiment carried out for animal is reality emerging in pathological study under Medical science mesolow hypoxia condition
Proved recipe method.
Current cell experiment device is more complicated during experimental implementation, is unfavorable for experimenter's operation, influences reality
Test result.
Summary of the invention
The object of the present invention is to provide a kind of observable hypobaric hypoxia cell incubators, under the conditions of hypobaric hypoxia
Cell experiment research, and convenient for the observation of experimenter's progress cell growth condition.
The technical scheme adopted by the invention is that a kind of observable hypobaric hypoxia cell incubator, including cabinet body, cabinet body
Top is provided with sealing cover, and bottom of cabinet bulk is provided with constant temperature blending instrument, and constant temperature blending instrument upper surface is provided with cell vessel, constant temperature
Blending instrument runs through bottom of cabinet bulk, constant temperature blending instrument and cabinet body fixed seal connection, and cell vessel are located in cabinet body；Cabinet body leads to respectively
Pipeline is crossed to connect with vaccum-pumping equipment, gas transportation facilities；Nutrient delivery device and observation device are additionally provided on cabinet body；
Observing device includes microscope.
The features of the present invention also characterized in that
Cabinet body inner wall is provided with limited block close to bottom, and limited block is close to constant temperature blending instrument upper surface.
Vaccum-pumping equipment includes connecting tube, and connecting tube one end passes through cabinet body side wall, is connected to interior of the cabinet, connecting tube is another
End is connect with vacuum pumping pump；
Gas transportation facilities include oxygen conveying pipe, nitrogen delivery pipe and carbon dioxide delivery pipe, oxygen conveying pipe, nitrogen
Delivery pipe and carbon dioxide delivery pipe one end pass through cabinet body side wall, are connected to interior of the cabinet, oxygen conveying pipe, nitrogen delivery pipe and
The carbon dioxide delivery pipe other end is connected with corresponding gas storage facility.
Flow control valve is respectively arranged in oxygen conveying pipe, nitrogen delivery pipe and carbon dioxide delivery pipe.
Sealing cover is connected through a screw thread with cabinet body.
Handle is provided at the top of sealing cover.
Cabinet body surface is provided with display, and temperature inductor, humidity sensor and pressure inductor are provided in cabinet body, temperature
Inductor, humidity sensor and pressure inductor is spent to be electrically connected with display respectively.
Nutrient delivery device includes injector, and injector ejection end runs through cabinet body side wall, protrudes into cabinet body, injector is another
End is connect with feeding solution.
Microscope is arranged at the top of expansion bracket, and expansion bracket bottom is fixedly connected with plate, plate side and 1 side wall bottom of cabinet body
Portion is fixedly connected, and micro- sem observation end is protruded into cabinet body.
It is to have PC plate on cabinet body side wall, the through-hole being adapted with micro- sem observation end, micro- sem observation is offered in PC plate
End via through holes protrude into cabinet body.
The invention has the advantages that
1, the present invention is put into cell vessel by setting cabinet body, sealing cover and handle convenient for opening cabinet body；By the way that oxygen is arranged
Letter shoot, nitrogen delivery pipe and carbon dioxide delivery pipe, convenient for survival state of the cell under various gas contents；By setting
Temperature inductor, humidity sensor and pressure inductor are set, convenient for detection cell growing environment；By the way that constant temperature blending instrument is arranged,
Convenient for controlling the temperature of interior of the cabinet, to achieve the effect that convenient for carrying out cell experiment under low-oxygen environment.
2, the present invention is pressurized and is depressurized convenient for interior of the cabinet by setting connecting tube and vacuum pumping pump；It is sprayed by setting
Device and feeding solution grow the cell on cell vessel convenient for the liquid that has additional nutrients；Pass through setting plate, expansion bracket and micro-
Mirror, convenient for observing the cell growth condition of interior of the cabinet, to achieve the effect that convenient for cell growth assay under low pressure.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of observable hypobaric hypoxia cell incubator of the present invention；
Fig. 2 is a kind of side structure schematic view of observable hypobaric hypoxia cell incubator of the present invention.
In figure, 1. cabinet bodies, 2. thread grooves, 3. sealing covers, 4. handles, 5. oxygen conveying pipes, 6. nitrogen delivery pipes, 7. dioxies
Change carbon delivery pipe, 8. temperature inductors, 9. humidity sensors, 10. pressure inductors, 11. constant temperature blending instruments, 12. limited blocks,
13. cell vessel, 14. connecting tubes, 15. vacuum pumping pumps, 16. injectors, 17. feeding solutions, 18. plates, 19. expansion brackets, 20.
Microscope.
Specific embodiment
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments.
A kind of structure of observable hypobaric hypoxia cell incubator of the present invention, as shown in Figure 1, 2, including cabinet body 1, cabinet body
1 top is provided with sealing cover 3, and 1 inner surface of cabinet body is provided with thread groove 2 close to top, and 3 surface of sealing cover is provided with and thread groove
2 corresponding screw threads, sealing cover 3 and cabinet body 1 are threadedly engaged sealed connection.1 bottom of cabinet body is provided with constant temperature blending instrument 11, permanent
Warm 11 upper surface of blending instrument is provided with cell vessel 13, and constant temperature blending instrument 11 runs through 1 bottom of cabinet body, constant temperature blending instrument 11 and cabinet body
1 fixed seal connection, cell vessel 13 are located in cabinet body 1；Cabinet body 1 is set by pipeline and vaccum-pumping equipment, gas conveying respectively
Standby connection；Nutrient delivery device and observation device are additionally provided on cabinet body 1；
Observation device includes microscope 20, and the setting of microscope 20 is in 19 top of expansion bracket, 19 bottom of expansion bracket and plate 18
It is fixedly connected, 18 side of plate is fixedly connected with 1 sidewall bottom of cabinet body, and microscope 20 is observed end and protruded into cabinet body 1.
1 inner wall surrounding of cabinet body is provided with limited block 12 close to bottom, and limited block 12 is close to 11 upper surface of constant temperature blending instrument.Limit
Position block 12 is used to limit the placement location of cell vessel 13, and plays stabilization.
Vaccum-pumping equipment includes connecting tube 14, and 14 one end of connecting tube passes through 1 side wall of cabinet body, and is connected to inside cabinet body 1, is connected
14 other end of pipe is connect with vacuum pumping pump 15；By the way that vaccum-pumping equipment is arranged, convenient for being increased in cabinet body inner cell experimentation
Pressure and decompression.
Gas transportation facilities include oxygen conveying pipe 5, nitrogen delivery pipe 6 and carbon dioxide delivery pipe 7, oxygen conveying pipe 5,
Nitrogen delivery pipe 6 and 7 one end of carbon dioxide delivery pipe pass through 1 side wall of cabinet body, and are connected to inside cabinet body 1, oxygen conveying pipe 5, nitrogen
Letter shoot 6 and 7 other end of carbon dioxide delivery pipe are connected with corresponding gas storage facility.
Oxygen conveying pipe 5, nitrogen delivery pipe 6 and carbon dioxide delivery pipe 7 are respectively arranged with flow control valve.Pass through setting
Gas transportation facilities, convenient for survival state of the detection cell under various gas contents.
Handle 4 is provided at the top of sealing cover 3.
1 surface of cabinet body is provided with display, and temperature inductor 8, humidity sensor 9 and pressure sensitive are provided in cabinet body 1
Device 10, temperature inductor 8, humidity sensor 9 and pressure inductor 10 are electrically connected with display respectively.By the way that temperature sense is arranged
Device 8, humidity sensor 9 and pressure inductor 10 are answered, convenient for detection cell growing environment situation.
Nutrient delivery device includes injector 16, and 16 ejection end of injector runs through 1 side wall of cabinet body, protrudes into cabinet body 1, is sprayed
16 other end of device is connect with feeding solution 17.Nutrient delivery device can be such as common push type by watering can used of watering flowers
Injection apparatus is also possible to the squash type injection apparatus of such as laboratory wash bottle.By the way that nutrient delivery device is arranged, convenient for increasing
Interior of the cabinet nutrient solution makes the carry out cell growth in cell vessel.
It is to have PC plate on 1 side wall of cabinet body, is offered in PC plate and observe the adaptable through-hole in end, microscope 20 with microscope 20
Observation end via through holes protrude into cabinet body 1.Device is observed by setting, convenient for observing the cell growth condition of interior of the cabinet.
Sealing cover 3 is driven to open cabinet body 1 by the rotation of handle 4 when use, and the cell vessel 13 that idioblast is housed
The constant temperature being placed in 1 inner bottom wall of cabinet body is mixed on instrument 11, and lock sealing lid 3, while defeated with oxygen conveying pipe 5, nitrogen is closed
Pipe 6 and carbon dioxide delivery pipe 7 are sent, the air inside cabinet body 1 is extracted by vacuum pumping pump 15, and cabinet is observed by microscope 20
The case where cell division inside body 1, and have additional nutrients liquid and oxygen and pressure appropriate, to reach convenient in hypoxemia
Cell growth assay is carried out under environment under low pressure.
Claims (10)
1. a kind of observable hypobaric hypoxia cell incubator, which is characterized in that including cabinet body (1), set at the top of the cabinet body (1)
It is equipped with sealing cover (3), cabinet body (1) bottom is provided with constant temperature blending instrument (11), and constant temperature blending instrument (11) upper surface is provided with cell
Vessel (13), constant temperature blending instrument (11) run through cabinet body (1) bottom, and constant temperature blending instrument (11) and cabinet body (1) are tightly connected, organelle
Ware (13) is located in cabinet body (1)；Cabinet body (1) is connect by pipeline with vaccum-pumping equipment, gas transportation facilities respectively；Cabinet body (1)
On be additionally provided with nutrient delivery device and observation device；
The observation device includes microscope (20).
2. a kind of observable hypobaric hypoxia cell incubator according to claim 1, which is characterized in that the cabinet body
(1) inner wall is provided with limited block (12) close to bottom, and limited block (12) is close to constant temperature blending instrument (11) upper surface.
3. a kind of observable hypobaric hypoxia cell incubator according to claim 1, which is characterized in that described to vacuumize
Equipment includes connecting tube (14), and connecting tube (14) one end passes through cabinet body (1) side wall, is connected to cabinet body (1) inside, connecting tube (14)
The other end is connect with vacuum pumping pump (15)；
The gas transportation facilities include oxygen conveying pipe (5), nitrogen delivery pipe (6) and carbon dioxide delivery pipe (7), and oxygen is defeated
It send pipe (5), nitrogen delivery pipe (6) and carbon dioxide delivery pipe (7) one end to pass through cabinet body (1) side wall, connects with cabinet body (1) inside
Logical, oxygen conveying pipe (5), nitrogen delivery pipe (6) and carbon dioxide delivery pipe (7) other end connect with corresponding gas storage facility
It connects.
4. a kind of observable hypobaric hypoxia cell incubator according to claim 3, which is characterized in that the oxygen is defeated
Pipe (5), nitrogen delivery pipe (6) and carbon dioxide delivery pipe (7) is sent to be respectively arranged with flow control valve.
5. a kind of observable hypobaric hypoxia cell incubator according to claim 1, which is characterized in that sealing cover (3)
It is connected through a screw thread with cabinet body (1).
6. a kind of observable hypobaric hypoxia cell incubator according to claim 1, which is characterized in that the sealing cover
(3) top is provided with handle (4).
7. a kind of observable hypobaric hypoxia cell incubator according to claim 1-6, which is characterized in that institute
It states cabinet body (1) surface and is provided with display, be provided with temperature inductor (8), humidity sensor (9) and feeling of stress in cabinet body (1)
It answers device (10), temperature inductor (8), humidity sensor (9) and pressure inductor (10) are electrically connected with display respectively.
8. a kind of observable hypobaric hypoxia cell incubator according to claim 1-6, which is characterized in that institute
Stating nutrient delivery device includes injector (16), and injector (16) ejection end runs through cabinet body (1) side wall, is protruded into cabinet body (1), spray
Emitter (16) other end is connect with feeding solution (17).
9. a kind of observable hypobaric hypoxia cell incubator according to claim 1-6, which is characterized in that institute
Microscope (20) setting is stated at the top of expansion bracket (19), expansion bracket (19) bottom is fixedly connected with plate (18), plate (18) side
Face is fixedly connected with 1 sidewall bottom of cabinet body, and microscope (20) observation end is protruded into cabinet body (1).
10. according to a kind of described in any item observable hypobaric hypoxia cell incubators of claim 9, which is characterized in that institute
Stating on cabinet body (1) side wall is to have PC plate, and the through-hole adaptable with microscope (20) observation end, microscope (20) are offered in PC plate
Observation end via through holes protrude into cabinet body (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910689648.7A CN110452819A (en) | 2019-07-29 | 2019-07-29 | A kind of observable hypobaric hypoxia cell incubator |
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| CN201910689648.7A CN110452819A (en) | 2019-07-29 | 2019-07-29 | A kind of observable hypobaric hypoxia cell incubator |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111205973A (en) * | 2020-02-25 | 2020-05-29 | 合肥职业技术学院 | Adjustable bacteria incubator and use method thereof |
| CN112226366A (en) * | 2020-10-21 | 2021-01-15 | 西北民族大学 | Low oxygen cell culture device |
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Application publication date: 20191115 |