CN110448702A - A kind of biomembrane inhibits and sweep-out method - Google Patents
A kind of biomembrane inhibits and sweep-out method Download PDFInfo
- Publication number
- CN110448702A CN110448702A CN201910834439.7A CN201910834439A CN110448702A CN 110448702 A CN110448702 A CN 110448702A CN 201910834439 A CN201910834439 A CN 201910834439A CN 110448702 A CN110448702 A CN 110448702A
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- Prior art keywords
- biomembrane
- microvesicle
- suspension
- substrate surface
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C19/00—Dental auxiliary appliances
- A61C19/002—Cleaning devices specially adapted for dental instruments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/025—Ultrasonics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
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- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dentistry (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The method that the invention discloses a kind of to inhibit and remove for biomembrane, belongs to sterilization field.The method realizes the inhibition and removing of biomembrane using cavitation effect, it is characterized in particular in and a kind of microvesicle suspension is provided, it includes microvesicle, antibacterial agent and solvents, by microvesicle suspension and the biofilm contact for being grown in substrate surface, make microvesicle suspension that biomembrane be completely covered, then cavitation technique processing is applied to microvesicle suspension and biomembrane, is inhibited and removed with the biomembrane to the substrate surface.The present invention is application of the microvesicle in biomembrane inhibits and removes, and especially provides experiment basis to the removal of narrow space biomembrane, provides theoretical foundation to prepare efficient, safety biological membrane removal preparation.
Description
Technical field
The invention belongs to physical sterilization fields, and in particular to a method of inhibit about biomembrane and removes.
Background technique
Biomembrane refer to be attached on substrate surface have special construction, organized bacterial community, it can be on surface
Secretion polysaccharide matrix, the formation such as lipid protein have levels, have extracellular polymeric (the extracellular polymeric of sequence
Substance, EPS) to play the role of protecting biomembrane.In real life, many substrate (living tissues, Medical treatment device
Tool, engineering material) in can all generate biomembrane, seriously threaten people's health.Research shows that about 80% in all medicine infection
Biomembrane from pathogen, and it is also the major reason that drug resistance is generated after causing bacterium infection.
Biomembrane and free bacterium have the characteristics that dramatically different, such as morphosis, pathogenic, toxicity, sensibility etc..Especially
Itself it is worth noting that, biofilm surface have one layer of fine and close extracellular polymeric to envelope bacterium formed be effectively protected.This
In the case of, currently used antibiotic or disinfectant processing can only often kill those free bacteriums of biofilm surface, and
It cannot be fully penetrated to the depths of biomembrane generation therapeutic effect.In fact, compared with free bacterium, the drug-resistant intensity of biomembrane
Up to 1000 times, due to the presence of extracellular polymeric, it can preferably escape body immune system and antibiotic generates
Killing.The biomembrane in addition to this, generated for narrow space, conventional physical means and chemical means processing (chemical flush,
Ultrasound, which is swung, washes) effective therapeutic effect can not be generated, treatment is got up also more added with challenge.Therefore, the treatment of biomembrane infection
Have become the clinically most thorny issue, with being continuously increased for bacterial drug resistance, the risk for the treatment of failure persistently increases.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of method safely and effectively biomembrane removal and inhibited.
Technical problem solved by the present invention is being reduced using physical means thin as caused by biofilm cells epimatrix
Bacterium drug resistance, to inhibit and remove with effectively realizing biomembrane.
To achieve the above object of the invention, the present invention is achieved through the following technical solutions:
1) a kind of microvesicle suspension is provided, it includes microvesicle, antibacterial agent and solvents；
2) by microvesicle suspension and the biofilm contact for being grown in substrate surface, make microvesicle suspension that biomembrane be completely covered；
3) cavitation technique processing is applied to microvesicle suspension and biomembrane, is inhibited with the biomembrane to the substrate surface
And removing.
Wherein, the frequency of the ultrasonic treatment is 20kHz~2000kHz, and the mechanical index of the ultrasound is 0.2~2, place
The time of reason is 0.1~5min.
Biomembrane disclosed by the invention inhibits and the beneficial effect of sweep-out method is: using this physical technique of cavitation effect
Realize the inhibition and removing of biomembrane, it is not easy to be influenced by extracellular matrix, be not easy to produce drug resistance；In addition to this,
The present invention realizes the transmitting of physical energy using the cavitation effect of microvesicle, can efficiently destroy the cell of narrow space biomembrane
Epimatrix is conducive to antibacterials and enters inside biomembrane, generates effective biomembrane inhibition and elimination effect.
Detailed description of the invention
Fig. 1 is canalis radicis dentis surface biological film process schematic diagram in embodiment 1, the tooth that wherein 1- is infected by biomembrane, 2-
Phospholipid microbubble suspension, 3- root canal sonicator, the biomembrane in 4- canalis radicis dentis, 5- phospholipid microbubble.
Fig. 2 is the experimental result picture of canalis radicis dentis surface biological film process in embodiment 1
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
Embodiment one
The present invention provides a kind of phospholipid microbubble suspension, and the partial size of microvesicle is 2.4 ± 0.5um.The ingredient of packet gas in microvesicle
It is perfluoropropane；The solvent of microvesicle suspension is physiological saline；Antibacterial agent is vancomycin.
The inhibition of the canalis radicis dentis biomembrane and sweep-out method are as follows:
1) saline injection (control group) and phospholipid microbubble suspension in the canalis radicis dentis of the tooth 1 infected by biomembrane,
The concentration of microvesicle is 1*107/ ml, 5*107/ ml, 1*108/ ml under blowing and beating 20 repeatedly using syringe, guarantees that phospholipid microbubble is uniform
It is distributed in canalis radicis dentis.
2) root canal sonicator 3 is put into canalis radicis dentis
3) apply ultrasonic treatment, setting supersonic frequency is 42kHz, ultrasonic time 1min.
4) it then uses normal saline flushing canalis radicis dentis three times, microvesicle membrane material fragment and biomembrane is rinsed well
Realize the inhibition and removing of biomembrane.
Fig. 1 is the schematic diagram of above-mentioned ultrasonic treatment canalis radicis dentis biomembrane, as shown, under the stimulation of dental pulp guidance needle,
Microvesicle can be induced to generate cavitation effect.Since microvesicle has micron-sized size, it can be reached as liquid flows
The side of canalis radicis dentis biomembrane, microvesicle generate cavitation effect can in a manner of physics disrupting biofilm surface extracellular base
Matter, and kill the bacterium of a part；In addition to this, the antibacterial agent vancomycin in solution, which can also enter, has been destroyed cell
Inside the biomembrane of epimatrix, bacterium intracellular is killed, the final inhibition and removing for realizing canalis radicis dentis biomembrane.Experimental result such as Fig. 2
Shown, it can be seen from the figure that compare with control group, phospholipid microbubble group shows excellent biomembrane inhibitory effect, and with
The promotion of phospholipid microbubble concentration, the enhancing of biomembrane inhibitory effect, this has also proved the therapeutic effect for inhibiting to biomembrane and removing
It is to be realized by the cavitation effect of phospholipid microbubble.
Embodiment two
The present invention provides a kind of surfactant microvesicle suspension, and the partial size of microvesicle is 1.8 ± 0.4um.Packet gas in microvesicle
For air.The solvent of microvesicle suspension is physiological saline；Antibacterial agent is the hydrogen peroxide that mass fraction is 3%.
The inhibition of the medical apparatus surface biomembrane and sweep-out method are as follows:
1) medical instrument (surgical clamp) is placed in bracket, surfactant microvesicle suspension is then injected in bracket,
Microvesicle suspension is set not have medical instrument completely, the final final concentration of 1*10 for guaranteeing microvesicle6Then/ml stirs 2min, guarantee micro-
Bubble comes into full contact with medical instrument.
2) bracket is put into supersonic cleaning machine processing 10min.
3) it then uses distilled water flushing medical instrument 3 times, microvesicle membrane material fragment and biomembrane is rinsed well, Ji Keshi
The inhibition and removing of existing biomembrane.
In the experiment of ultrasonic treatment medical apparatus surface biomembrane, under the action of supersonic cleaning machine, support can be induced
Microvesicle in slot generates cavitation effect.Since microvesicle has micron-sized size, it can enter the narrow of medical instrument
Position.Later microvesicle generate cavitation effect can in a manner of physics disrupting biofilm surface extracellular matrix, and kill one
Partial bacterium；In addition to this, the antibacterial agent hydrogen peroxide in solution can also enter the life for being destroyed extracellular matrix
Inside object film, bacterium intracellular is killed, the final inhibition and removing for realizing medical apparatus surface biomembrane.
Claims (7)
1. a kind of method that biomembrane inhibits and removes, which is characterized in that realize the inhibition of biomembrane and clear using cavitation effect
It removes, the described method comprises the following steps:
1) a kind of microvesicle suspension is provided, it includes microvesicle, antibacterial agent and solvents；
2) by microvesicle suspension and the biofilm contact for being grown in substrate surface, make microvesicle suspension that biomembrane be completely covered；
3) ultrasonic cavitation technology processing is applied to microvesicle suspension and biomembrane, is inhibited with the biomembrane to the substrate surface
And removing.
2. the method according to claim 1, wherein the microvesicle be core-shell structure of the partial size at 1~20 μm, it is interior
Portion is gas, and outside is coated fertilizer.
3. according to the method described in claim 2, it is characterized in that, the coated fertilizer of the microvesicle is phosphatide, protein, surface
One or more of compositions of activating agent.
4. according to the method described in claim 2, it is characterized in that, the gas of the microvesicle be air, it is nitrogen, argon gas, complete
One or several kinds of compositions of fluoro-propane and sulfur hexafluoride.
5. the method according to claim 1, wherein the antibacterial agent be hydrogen peroxide, penicillin, erythromycin,
One or several kinds of compositions of vancomycin.
6. the method according to claim 1, wherein the solvent is water, physiological saline or phosphate-buffered
Liquid.
7. the method according to claim 1, wherein the ultrasonic cavitation technology processing frequency be 20kHz~
2000kHz, the mechanical index of the ultrasound are 0.2~2, and the time of processing is 0.1~5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910834439.7A CN110448702A (en) | 2019-09-05 | 2019-09-05 | A kind of biomembrane inhibits and sweep-out method |
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| CN201910834439.7A CN110448702A (en) | 2019-09-05 | 2019-09-05 | A kind of biomembrane inhibits and sweep-out method |
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| CN110448702A true CN110448702A (en) | 2019-11-15 |
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| CN201910834439.7A Pending CN110448702A (en) | 2019-09-05 | 2019-09-05 | A kind of biomembrane inhibits and sweep-out method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352901A (en) * | 2020-09-16 | 2021-02-12 | 浙江大学 | Application of synergy of mannosylerythritol lipids and ultrasound in inhibition of growth of drug-resistant bacteria |
| EP4054332A4 (en) * | 2019-11-08 | 2024-01-03 | Triangle Biotechnology, Inc. | Methods of using nanoparticles and microparticles for the disruption and prevention of biofilms |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050241668A1 (en) * | 2004-03-18 | 2005-11-03 | Andrej Trampuz | Microbial biofilm removal methods and systems |
| US20110256503A1 (en) * | 2008-12-30 | 2011-10-20 | Koninklijke Philips Electronics N.V. | Ultrasonic teeth cleaning appliance having spatial, temporal and/or frequency variations |
| CN107072884A (en) * | 2014-09-04 | 2017-08-18 | 西亚克特有限责任公司 | The root canal disinfection of microvesicle based on photochemical activation |
| WO2017145118A1 (en) * | 2016-02-25 | 2017-08-31 | King Abdullah University Of Science And Technology | Acoustically excited encapsulated microbubbles and mitigation of biofouling |
-
2019
- 2019-09-05 CN CN201910834439.7A patent/CN110448702A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050241668A1 (en) * | 2004-03-18 | 2005-11-03 | Andrej Trampuz | Microbial biofilm removal methods and systems |
| US20110256503A1 (en) * | 2008-12-30 | 2011-10-20 | Koninklijke Philips Electronics N.V. | Ultrasonic teeth cleaning appliance having spatial, temporal and/or frequency variations |
| CN107072884A (en) * | 2014-09-04 | 2017-08-18 | 西亚克特有限责任公司 | The root canal disinfection of microvesicle based on photochemical activation |
| WO2017145118A1 (en) * | 2016-02-25 | 2017-08-31 | King Abdullah University Of Science And Technology | Acoustically excited encapsulated microbubbles and mitigation of biofouling |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4054332A4 (en) * | 2019-11-08 | 2024-01-03 | Triangle Biotechnology, Inc. | Methods of using nanoparticles and microparticles for the disruption and prevention of biofilms |
| CN112352901A (en) * | 2020-09-16 | 2021-02-12 | 浙江大学 | Application of synergy of mannosylerythritol lipids and ultrasound in inhibition of growth of drug-resistant bacteria |
| CN112352901B (en) * | 2020-09-16 | 2022-06-10 | 浙江大学 | Application of synergy of mannosylerythritol lipids and ultrasound in inhibition of growth of drug-resistant bacteria |
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