CN110447647A - One plant growth regulators and its application - Google Patents
One plant growth regulators and its application Download PDFInfo
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- CN110447647A CN110447647A CN201910752014.1A CN201910752014A CN110447647A CN 110447647 A CN110447647 A CN 110447647A CN 201910752014 A CN201910752014 A CN 201910752014A CN 110447647 A CN110447647 A CN 110447647A
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- plant growth
- active components
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- growth regulator
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to agricultural plant protection fields, adjust and apply in particular to a plant growth.Activities of Plant Growth Regulators group is divided into active components A and active constituent B, and active components A 2,4- difluorobenzoyl aminoisobutyric acid, active constituent B is chitosan oligosaccharide；Wherein, the weight ratio of active components A and active constituent B are as follows: 1-40:20-2000.For this hair plant growth regulator composition compared with single dose, effect is more significant, can enhance crops resistance, promotes plant growth, increases crop yield, improves crops fruit quality.
Description
Technical field
The present invention relates to agricultural plant protection fields, adjust and apply in particular to a plant growth.
Background technique
Plant growth regulator is a kind of hormonal substance for being widely used in coordinate plant growth development in agricultural production,
The growth and development of plant can effectively be regulated and controled, enhance resistance, improve yield, improving quality, and with dosage is small, takes effect
Fastly, the feature of residual less, with strong points.Plant growth promoter, plant growth inhibitor can be divided into the effect of growth by it
And plant growth retardent, difference is selected according to plant variety and application purpose.Common growth promoter have indolepopionic acid,
Methyl α-naphthyl acetate, 6-benzyladenine；Common growth inhibitor has Triiodobenzoic acid, maleic hydrazide；Common growth retardant has alkene
Imitate azoles, paclobutrazol and cycocel.The study found that two or more plant growth regulator is used in mixed way or successively makes
With often more effect is administered alone in generation ratio, preferably plays its regulating and controlling effect；But it also will appear two kinds or two kinds
Above plant growth regulator is used in mixed way the growth for inhibiting crop, and then leads to the lower or output increased of crop yield
Amplitude be less than the using effect of single dose, therefore the reasonable employment of plant growth regulating is vital.
In recent years, plant growth regulator is increasingly taken seriously, and can not only reduce agricultural cost, and can be improved work
Object growth and yield, improve fruit quality and fertilizer utilization efficiency, additionally it is possible to which soil protection environment reduces the generation of disease.
Summary of the invention:
It adjusts and applies it is an object of the present invention to provide a plant growth.
To achieve the above object, the invention adopts a technical scheme as:
One plant growth regulators, Activities of Plant Growth Regulators group are divided into active components A and active constituent B, activity
Ingredient A is 2,4- difluorobenzoyl aminoisobutyric acid, and active constituent B is chitosan oligosaccharide；Wherein, active components A and active constituent B
Weight ratio are as follows: 1-40:20-2000.
The structural formula of the active components A 2,4- difluorobenzoyl aminoisobutyric acid is
Molecular mass is 243.21.
2, the 4- difluorobenzoyl aminoisobutyric acid can be according to application No. is record in 2018113654505 patent documents
Synthetic method prepare, further synthesis step is as follows:
7.0 grams of 2,4- difluoro-benzoic acid are added in 250mL flask, EDCI (1- ethyl-(3- dimethylaminopropyl) carbon
Acyl diimmonium salt hydrochlorate) 10.0 grams, 7.5 grams of aminoisobutyric acid methyl ester hydrochloride, methylene chloride 150mL, triethylamine 5mL.Room temperature
It is stirred to react 12 hours.After completion of the reaction, reaction solution is washed with water 50mL × 3, and organic phase is 12 hours dry with anhydrous sodium sulfate,
Precipitation is depressurized, ethyl alcohol 150mL is added, 7.0 grams of sodium hydroxide, reaction 2 hours is stirred at room temperature.TLC monitors decompression after completion of the reaction
Precipitation, residue add water 100mL, are extracted with ethyl acetate 50mL × 3, and organic phase is 12 hours dry with anhydrous sodium sulfate, decompression
Precipitation, column chromatography (eluant, eluent is ethyl acetate) purify to obtain compound 2,4- difluorobenzoyl aminoisobutyric acid, white solid 3.1
Gram.1H NMR (600MHz, DMSO) δ 12.63 (s, 1H), 8.55 (s, 1H), 7.85 (d, J=8.4Hz, 1H), 7.24 (d, J=
1.3Hz, 1H), 7.12 (dd, J=8.4,1.6Hz, 1H), 1.52 (s, 6H).
It is preferred that the weight ratio of the active components A and active constituent B are as follows: 1-20:50-1000.
Further preferably, the weight ratio of the active components A and active constituent B are as follows: 1-10:100-1000.
The active component, and agriculturally acceptable auxiliary agent or carrier；Wherein, the concentration range of active components A is
The concentration range of 0.005-0.2mg/L, active constituent B are 0.1-10mg/L.
The concentration range of the active components A is 0.01-0.2mg/L, and the concentration range of active constituent B is 0.5-10mg/
L。
The concentration range of the active components A is 0.01-0.1mg/L, and the concentration range of active constituent B is 1-10mg/L.
The dosage form of the regulator is aqua, wettable powder, water dispersible granules, oil-suspending agent, suspoemulsion, missible oil, micro-
Emulsion, aqueous emulsion, soluble granule or solvable pulvis.
It is preferred that the dosage form of the regulator is aqua, wettable powder, solvable pulvis, suspoemulsion.
The application of one plant growth regulators, the plant growth regulator is for regulating and controlling answering in plant growth
With.
The crop is to apply on wheat, rice, peanut, potato or capsicum.
Advantage for present invention are as follows:
Activities of Plant Growth Regulators ingredient chitosan oligosaccharide of the invention and 2,4- difluorobenzoyl aminoisobutyric acid can expire
The growth demand of sufficient plant promotes plant growth and development, improves the yield of plant, improves crop anti-adversity, improves crop quality
The effects of, and will not be remained in plant again, improve economic and social benefit；Specifically:
1. regulator active constituent of the present invention is 2,4- difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide, according to weight ratio 1-
40:20-2000 is compounded has significant synergistic function in terms of adjusting or promoting plant growth, can be improved crop
Resistance and crop yield, improve crop quality, improve economic and social benefit.
2. the frequency of use and usage amount of plant growth regulator of the invention simultaneously are with crops, growing environment, weather
Weather and it is small change, volume increase can be achieved the purpose that by using suitable dosage.
3. plant growth regulator of the invention can be entered in plant by the stomata of plant leaf blade, and pass through plant
The intracorporal physiological action of object is decomposed, and will not be remained in plant body.
4. plant growth regulator of the invention can promote the raising of the chlorophyll content of plant, photosynthetic efficiency is improved,
Be conducive to the accumulation of plant dry matter, further promote the raising of yield.
Specific embodiment:
The present invention is further explained in the light of specific embodiments, but the invention is not limited to following embodiment party
Formula, one skilled in the art would recognize that all alternative present invention encompasses what be may include in Claims scope
Scheme, improvement project and equivalent scheme.
Example of formulations
By the preparation method of each preparation of existing routine, each preparation is now with aqua using above-mentioned composition as active constituent
Example, accounts for 0.000101-0.00101% according to the percentages active constituent of weight, auxiliary material is matched according to the percentage of weight
System, wherein active components A and B are matched according to various concentration, account for the percentage (table 1) of preparation in bracket for active constituent.
Table 1:2,4- difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide compounding
The additional amount of auxiliary material is in each composition, neopelex 4%, citric acid 1%, polysorbate 3%,
Butylphosphoric acid ester 4%, ethylene glycol 2%, pure water complement to 100%.
Application examples
1) indoor pot experiment is verified
Subjects: the small sharp green pepper of loud, high-pitched sound (the Harbin agriculture Co., Ltd of gold dragon provides)
Test method: test carries out in Shenyang Chemical Research Institute's glasshouse.By turfy soil (Shenyang South Lake flowers market
Purchase) be uniformly mixed and be filled in 54cm*27cm*6cm seedlings nursing plate with sand 1:1 ratio in mass ratio, seed is uniformly sowed to
Seedlings nursing plate is interior and covers the soil layer of about 0.5cm thickness, waters from bottom, seed hair is carried out under the conditions of 24-28 DEG C of great abundance of sunshine
Bud nursery.The consistent pepper seedling of growing way is selected after pepper plant length to 2 true leaves to transplant into flowerpot, keeps every basin Miao Sheng
Elongate member is consistent, applies base fertilizer after slow seedling, base fertilizer is routine compound fertilizer 15-15-15, by dosage 600kg/hm2Application.After transplanting
Progress first time medicament sprays within 15 days, and carries out second of medicament after 30 days and spray, and investigates yield of hot pepper in fruiting period, each
5 repetitions are handled, test result is shown in Table 2.
Medicament is experiment group reagent, control group reagent and blank；Wherein, experimental group is chitosan oligosaccharide and 2,4- difluorobenzoyl
The aqua that aminoisobutyric acid is recorded by above-described embodiment of different compoundings；Control group is various concentration chitosan oligosaccharide or 2,4- difluorobenzene
Formamido group isobutyric acid；Blank is clear water.
Table 2: capsicum pot experiment determination data
As shown in Table 2, pot experiment the result shows that foliage-spray chitosan oligosaccharide and 2,4- difluorobenzoyl aminoisobutyric acid
The raising of promotion yield of hot pepper that can be different degrees of, and the compound aqueous effect in this research becomes apparent.By number in table
According to it is found that when 2,4- difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide proportion be 1:100 effect it is the most significant, when spray shell widow
Sugared concentration is 1mg/L, and when 2,4- difluorobenzoyl aminoisobutyric acid concentrations are 0.01mg/L, capsicum volume increase is the most obvious, volume increase
Effect is higher than each single dose and other complex treatments, has obvious synergistic function.
2) capsicum field trial is verified
Subjects: capsicum
Test method: test was carried out in 2018 in Shenyang City Tiexi District.Pepper seed is uniformly sowed to seedlings nursing plate,
The fine earth for covering one layer of 0.5-1cm thickness, is then watered with water.The consistent pepper seedling of growing way is selected when pepper plant long 12cm,
It is transplanted in ground.
Experimental design: 3 repetitions are arranged in the long 5m in experimental plot, wide 3m, the random district's groups arrangement of cell, each test process.
According to the test result of potting, experimental group is that (2,4- difluorobenzoyl amino are different for selection active components A in above-described embodiment aqua
Butyric acid) and active constituent B (chitosan oligosaccharide) by various concentration carry out compounding prepare aqua；Control group is various concentration simultaneously
Active component A or active component B；Blank is clear water；It carries out spraying different disposal medicament for the first time after capsicum slow seedling, after 30 days
It is sprayed for the second time, carries out yield of hot pepper measurement to harvest time, test result is shown in Table 3.
Table 3: capsicum field trial determination data
According to table 3, the survey in capsicum crop field produces result and the trend of pot experiment is essentially identical, and the effect compounded is more
It is obvious.Under the conditions of 6 compoundings, the yield of capsicum is dramatically increased, and amount of increase in production is all larger than the effect for spraying single dose, wherein 2,
The effect of increasing production that 4- difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide proportion are 1:100 is greater than other conditions of mixture ratios, and in 1:100
Conditions of mixture ratios under spray chitosan oligosaccharide concentration be 1mg/L, 2,4- difluorobenzoyl aminoisobutyric acid concentrations be 0.01mg/L when, it is peppery
Green pepper crop field effect of increasing production is the most significant, has obvious synergistic function.
3) peanut field verification experimental verification
Subjects: peanut
Test method: test was carried out in 2018 in Liaoning Province Fuxin City Zhangwu County.Test material is that green grind spends No. 3.
Experimental design: the long 4m in experimental plot, wide 1.65m, line-spacing 55cm, spacing in the rows 10cm, the random district's groups arrangement of cell, each
3 repetitions are arranged in test process.According to the test result of potting, experimental group is to choose active components A in above-described embodiment aqua
(2,4- difluorobenzoyl aminoisobutyric acid) and active constituent B (chitosan oligosaccharide) by various concentration carry out compounding prepare water
Agent；Control group is various concentration active component A or active component B simultaneously；Blank is clear water；Progress first time spray in after planting 15 days
It applies, is sprayed for the second time after 30 days, florescence measures the SPAD value of peanut leaf and the dry weight and fresh weight of plant seedlings of plant, and harvest time is spent
Output measurement, test result are shown in Table 4.
Table 4: peanut field trial determination data
According to table 4, the test result in peanut crop field shows foliage-spray chitosan oligosaccharide and 2,4- difluorobenzoyl amino
Isobutyric acid can promote the raising of peanut yield, and wherein the effect of the complex composition in this research becomes apparent.Wherein 2,4-
The effect of increasing production that difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide proportion are 1:100-1:200 is greater than other conditions of mixture ratios, wherein
It is 1mg/L that chitosan oligosaccharide concentration is sprayed under the conditions of mixture ratios of 1:100, and 2,4- difluorobenzoyl aminoisobutyric acid concentrations are
When 0.01mg/L, peanut chlorophyll content, dry matter content accumulation and effect of increasing production are the most obvious.
It can be seen that plant modifying agent 2 of the present invention, 4- difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide proportion are 1:100-
The effect of increasing production of crop is more obvious when 1:200, and spraying under 1:100 conditions of mixture ratios chitosan oligosaccharide concentration is 1mg/L, and 2,4- bis-
When fluorobenzoylamino isobutyl acid concentration is 0.01mg/L, the effect of increasing production of capsicum and peanut is the most significant, wherein trying in crop field
Middle capsicum volume increase 17.53% is tested, peanut yield increasing 15.62% illustrates that this plant growth regulator can promote the chlorophyll of plant
The raising of content improves photosynthetic efficiency, promotes the accumulation and transhipment of plant dry matter, and then leads to mentioning for peanut and yield of hot pepper
It is high.
Meanwhile may also act to above-mentioned acquisition aqua in other crops, have in terms of adjusting or promoting plant growth
Significant synergistic function can be improved the resistance and crop yield of crop, improve crop quality, improve economy and society
Benefit.
The above described specific embodiments of the present invention are not intended to limit the scope of the present invention..Any basis
Any other various changes and modifications made by technical concept of the invention should be included in the guarantor of the claims in the present invention
It protects in range.
Claims (10)
1. a plant growth regulators, it is characterised in that: Activities of Plant Growth Regulators group be divided into active components A and activity at
Divide B, active components A 2,4- difluorobenzoyl aminoisobutyric acid, active constituent B is chitosan oligosaccharide；Wherein, active components A and work
The weight ratio of property ingredient B are as follows: 1-40:20-2000.
2. plant growth regulator according to claim 1, it is characterised in that: the active components A 2,4- difluorobenzoyl
The structural formula of aminoisobutyric acid isMolecular mass is 243.21.
3. plant growth regulator according to claim 1, it is characterised in that: the weight of the active components A and active constituent B
Measure ratio are as follows: 1-20:50-1000.
4. plant growth regulator according to claim 3, it is characterised in that: the weight of the active components A and active constituent B
Measure ratio are as follows: 1-10:100-1000.
5. by plant growth regulator described in claim 1-4 any one, it is characterised in that: the claim 1-4 appoints
It anticipates an active component, and agriculturally acceptable auxiliary agent or carrier；Wherein, the concentration range of active components A is 0.005-
The concentration range of 0.2mg/L, active constituent B are 0.1-10mg/L.
6. plant growth regulator as described in claim 5, it is characterised in that: the concentration range of the active components A is
The concentration range of 0.01-0.2mg/L, active constituent B are 0.5-10mg/L.
7. plant growth regulator according to claim 6, it is characterised in that: the concentration range of the active components A is
The concentration range of 0.01-0.1mg/L, active constituent B are 1-10mg/L.
8. plant growth regulator as described in claim 5, it is characterised in that: the dosage form of the regulator is aqua, wettable
Property pulvis, water dispersible granules, oil-suspending agent, suspoemulsion, missible oil, microemulsion, aqueous emulsion, soluble granule or solvable pulvis.
9. a kind of application of the plant growth regulator of claim 1-5 any one, it is characterised in that: the plant
Growth regulator is for regulating and controlling the application in plant growth.
10. the application of plant growth regulator according to claim 9, it is characterised in that: the crop be wheat, rice,
It is applied on peanut, potato or capsicum.
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| CN201910752014.1A CN110447647B (en) | 2019-08-15 | 2019-08-15 | Plant growth regulator and application thereof |
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| CN201910752014.1A CN110447647B (en) | 2019-08-15 | 2019-08-15 | Plant growth regulator and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112006037A (en) * | 2020-09-04 | 2020-12-01 | 安徽省四达农药化工有限公司 | Plant growth regulator containing chitosan oligosaccharide and application thereof |
| CN114729016A (en) * | 2019-11-27 | 2022-07-08 | 南京纽邦生物科技有限公司 | Enzyme mutant for producing 3-aminoisobutyric acid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585175A (en) * | 2015-01-21 | 2015-05-06 | 合肥工业大学 | Compound preparation capable of improving virus resistance of plants, and application of compound preparation |
-
2019
- 2019-08-15 CN CN201910752014.1A patent/CN110447647B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585175A (en) * | 2015-01-21 | 2015-05-06 | 合肥工业大学 | Compound preparation capable of improving virus resistance of plants, and application of compound preparation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114729016A (en) * | 2019-11-27 | 2022-07-08 | 南京纽邦生物科技有限公司 | Enzyme mutant for producing 3-aminoisobutyric acid |
| CN114729016B (en) * | 2019-11-27 | 2022-12-13 | 南京纽邦生物科技有限公司 | Enzyme mutant for producing 3-aminoisobutyric acid |
| CN112006037A (en) * | 2020-09-04 | 2020-12-01 | 安徽省四达农药化工有限公司 | Plant growth regulator containing chitosan oligosaccharide and application thereof |
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