CN110426514A - The novel measuring of acyltransferase polypeptide arginine deiminase (PAD) method living - Google Patents
The novel measuring of acyltransferase polypeptide arginine deiminase (PAD) method living Download PDFInfo
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- CN110426514A CN110426514A CN201910801766.2A CN201910801766A CN110426514A CN 110426514 A CN110426514 A CN 110426514A CN 201910801766 A CN201910801766 A CN 201910801766A CN 110426514 A CN110426514 A CN 110426514A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) methods living; including reagent; the reagent includes Streptavidin; the aqueous solution of FBNA-2 peptide; PAD enzyme sample; trypsase, rinse liquid, PAD buffer; trypsase buffer; the anti-6xHIS antibody of HRP label, tetramethyl benzidine (TMB) substrate solution, 2M sulfuric acid; the Streptavidin is coated with 8 hole battens; the aqueous solution of the FBNA-2 peptide is 0.5mg/ml, and the trypsase, 10mg/ml is dissolved in Low acid storing liquid；Present invention incorporates the advantages for the nearly all current PAD measuring method for activity enumerated in first part, the especially high sensitivity of immunoassay, ease for operation, with the low-cost advantage of Trypsin method, in addition to the novel substrate in conceptual design, other all reagents are biology laboratory common agents, thus are easy to obtain.
Description
Technical field
The invention belongs to technical fields, and in particular to the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) method living.
Background technique
Acyltransferase polypeptide arginine deiminase (Peptidylarginine deiminases, PADs) is a kind of dependent on Ca2+
And it is catalyzed the enzyme that acyltransferase polypeptide conversion of Arginine is acyltransferase polypeptide citrulling, which is made of in human cell 5 isodynamic enzymes,
The enzyme activity of PAD1-4 and PAD6.PAD is usually to be measured using the conversion of substrate as reading.
Existing PAD measuring method for activity has certain problem, for example, colorimetric analysis only to the PAD albumen of purifying into
The quick enzyme activity determination of row, the cumbersome time-consuming of HPLC fluorescence analysis are not suitable for detecting multiple sample simultaneously, PAD immunoassay
The problems such as acquisition specific antibody is at high cost, has sensitivity bottom, and operability is poor, at high cost, thus it is proposed that acyltransferase polypeptide essence ammonia
The novel measuring of acid de- imines enzyme (PAD) method living.
Summary of the invention
It is above-mentioned to solve the purpose of the present invention is to provide the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) method living
The problems such as existing PAD measuring method for activity proposed in background technique has sensitivity bottom, and operability is poor, at high cost.
To achieve the above object, the invention provides the following technical scheme: acyltransferase polypeptide arginine deiminase (PAD) it is novel
Method living, including reagent are surveyed, the reagent includes Streptavidin, the aqueous solution of FBNA-2 peptide, PAD enzyme sample, trypsase, profit
Washing lotion, PAD buffer, trypsase buffer, the anti-6xHIS antibody of HRP label, tetramethyl benzidine (TMB) substrate solution,
2M sulfuric acid.
Further, the Streptavidin is coated with 8 hole battens, and the aqueous solution of the FBNA-2 peptide is 0.5mg/ml.
Further, the molecular sequences of the FBNA-2 peptide are as follows: Biotin-GGSGG SYSAQ FTSST SYNRG
DSTFE SHHHH HH。
Further, the trypsase, 10mg/ml are dissolved in Low acid storing liquid.
Further, the rinse liquid, 1x TBS (25mM Tris, 150mM NaCl, pH 7.2), 0.1%BSA and
0.05%Tween-20.
Further, the PAD buffer, 0.1M Tris-HCl (pH 7.4), 10mM CaCl2, use preceding addition
0.5mM DTT and 1mM PMSF, the trypsase buffer, 50mM Tris-HCl (pH8.0), 2.5mM EDTA.
Further, survey step living includes:
S1: rinse liquid is used to dilute FBNA-2 peptide to 0.5ug/ml as coating buffer；
S2: 8 hole battens are installed in elisa plate frame, plate is inverted on clean blotting paper and is clapped after rinse batten 3 times
It is dry；
S3: 100ul coating buffer being added into every hole, is incubated for 1 hour at room temperature；
S4: discarding the solution in batten, with plate being inverted on clean blotting paper and patted dry after the rinse of rinse liquid 4 times；
S5: enzyme is diluted to required concentration with PAD buffer, 50ul is added into every hole, batten is placed in 37 DEG C of incubations
30 minutes；
S6: step 4 is repeated；
S7: 100ul trypsase buffer is added in every hole, discards solution after balance；
S8: after trypsase is diluted 1000 times with buffer, add 100ul into each reacting hole of batten, be placed in 37
DEG C be incubated for 1 hour；
S9: step 4 is repeated；
S10: after HIS is detected antibody with 10 times of rinse liquid dilution, add 100ul into each reacting hole of batten, be placed in
Incubation at room temperature 1 hour；
S11: step 4 is repeated；
S12: after TMB solution equilibria to room temperature, add 100ul into each reacting hole of batten, be placed in room temperature, be protected from light ring
It is incubated for 20 minutes in border；
S13: adding 50ul2M sulfuric acid to react to reacting hole with color development stopping, slightly shakes batten, solution colour is by indigo plant at this time
Color is changed into yellow, reads the absorbance in every hole respectively in 450nm and 540nm in 30 minutes.
Compared with prior art, the beneficial effects of the present invention are:
1. present invention incorporates the advantages for the nearly all current PAD measuring method for activity enumerated in first part.Especially exempt from
The high sensitivity of epidemic disease analytic approach, ease for operation, the low-cost advantage with Trypsin method.In addition to the novel bottom in conceptual design
Object, other all reagents are biology laboratory common agents, thus are easy to obtain.This programme has been found to can be used for measuring
The activity of all 5 kinds of human genes coding albumen of PAD enzyme family.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is structural schematic diagram of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1 to Fig. 2, the present invention provides a kind of technical solution: acyltransferase polypeptide arginine deiminase (PAD) it is novel
Method living is surveyed, including reagent, reagent include Streptavidin, the aqueous solution of FBNA-2 peptide, PAD enzyme sample, trypsase, rinse
Liquid, PAD buffer, trypsase buffer, the anti-6xHIS antibody of HRP label, tetramethyl benzidine (TMB) substrate solution, 2M
Sulfuric acid.
Preferably, Streptavidin is coated with 8 hole battens, and the aqueous solution of FBNA-2 peptide is 0.5mg/ml.
Preferably, trypsase, 10mg/ml are dissolved in Low acid storing liquid.
Preferably, the molecular sequences of FBNA-2 peptide are as follows: Biotin-GGSGG SYSAQ FTSST SYNRG DSTFE
SHHHH HH。
Preferably, rinse liquid, 1x TBS (25mM Tris, 150mM NaCl, pH 7.2), 0.1%BSA and 0.05%
Tween-20。
Preferably, PAD buffer, 0.1M Tris-HCl (pH 7.4), 10mM CaCl2, use preceding addition 0.5mM DTT
And 1mM PMSF, trypsase buffer, 50mM Tris-HCl (pH 8.0), 2.5mM EDTA.
Preferably, surveying step living includes:
S1: rinse liquid is used to dilute FBNA-2 peptide to 0.5ug/ml as coating buffer；
S2: 8 hole battens are installed in elisa plate frame, plate is inverted on clean blotting paper and is clapped after rinse batten 3 times
It is dry；
S3: 100ul coating buffer being added into every hole, is incubated for 1 hour at room temperature；
S4: discarding the solution in batten, with plate being inverted on clean blotting paper and patted dry after the rinse of rinse liquid 4 times；
S5: enzyme is diluted to required concentration with PAD buffer, 50ul is added into every hole, batten is placed in 37C and is incubated for 30
Minute；
S6: step 4 is repeated；
S7: 100ul trypsase buffer is added in every hole, discards solution after balance；
S8: after trypsase is diluted 1000 times with buffer, add 100ul into each reacting hole of batten, be placed in 37
DEG C be incubated for 1 hour；
S9: step 4 is repeated；
S10: after HIS is detected antibody with 10 times of rinse liquid dilution, add 100ul into each reacting hole of batten, be placed in
Incubation at room temperature 1 hour；
S11: step 4 is repeated；
S12: after TMB solution equilibria to room temperature, add 100ul into each reacting hole of batten, be placed in room temperature, be protected from light ring
It is incubated for 20 minutes in border；
S13: adding 50ul2M sulfuric acid to react to reacting hole with color development stopping, slightly shakes batten, solution colour is by indigo plant at this time
Color is changed into yellow, reads the absorbance in every hole respectively in 450nm and 540nm in 30 minutes.
The working principle of the invention and process for using: the enzymatic activity of PAD makes the bottom being incorporated in 8 hole batten Avidins coating
Conversion of Arginine in object FBNA-2 peptide is citrulling, and the latter is not by trypsin hydrolysis, so as to make 6 histidines of C-terminal
Structure retains in the orifice plate.6 histidine structure is then in conjunction with specific antibody.The antibody by HRP because being marked, it is possible to
It is measured by ELISA method.Therefore, the absorbance finally read should be with the proportional linear relationship of PAD activity.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) method living, including reagent, it is characterised in that: the reagent includes
Streptavidin, the aqueous solution of FBNA-2 peptide, PAD enzyme sample, trypsase, rinse liquid, PAD buffer, trypsase buffering
Liquid, the anti-6xHIS antibody of HRP label, tetramethyl benzidine (TMB) substrate solution, 2M sulfuric acid.
2. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, it is characterised in that: institute
It states Streptavidin and is coated with 8 hole battens, the aqueous solution of the FBNA-2 peptide is 0.5mg/ml.
3. the novel measuring of the acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, feature exist
In: the molecular sequences of the FBNA-2 peptide are as follows: Biotin-GGSGG SYSAQ FTSST SYNRG DSTFE SHHHH HH.
4. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, it is characterised in that: institute
Trypsase is stated, 10mg/ml is dissolved in Low acid storing liquid.
5. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, it is characterised in that: institute
State rinse liquid, 1x TBS (25mM Tris, 150mM NaCl, pH 7.2), 0.1%BSA and 0.05%Tween-20.
6. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, it is characterised in that: institute
State PAD buffer, 0.1M Tris-HCl (pH 7.4), 10mM CaCl2, use preceding addition 0.5mM DTT and 1mM PMSF, institute
State trypsase buffer, 50mM Tris-HCl (pH 8.0), 2.5mM EDTA.
7. the novel measuring of acyltransferase polypeptide arginine deiminase (PAD) according to claim 1 method living, it is characterised in that: institute
Stating survey step living includes:
S1: rinse liquid is used to dilute FBNA-2 peptide to 0.5ug/ml as coating buffer；
S2: 8 hole battens are installed in elisa plate frame, plate is inverted on clean blotting paper and is patted dry after rinse batten 3 times；
S3: 100ul coating buffer being added into every hole, is incubated for 1 hour at room temperature；
S4: discarding the solution in batten, with plate being inverted on clean blotting paper and patted dry after the rinse of rinse liquid 4 times；
S5: enzyme is diluted to required concentration with PAD buffer, 50ul is added into every hole, batten is placed in 37C and is incubated for 30 points
Clock；
S6: step 4 is repeated；
S7: 100ul trypsase buffer is added in every hole, discards solution after balance；
S8: after trypsase is diluted 1000 times with buffer, add 100ul into each reacting hole of batten, be placed in 37 DEG C and incubate
It educates 1 hour；
S9: step 4 is repeated；
S10: after HIS is detected antibody with 10 times of rinse liquid dilution, add 100ul into each reacting hole of batten, be placed in room temperature
It is incubated for 1 hour；
S11: step 4 is repeated；
S12: after TMB solution equilibria to room temperature, adding 100ul into each reacting hole of batten, is placed in room temperature, in light protected environment
It is incubated for 20 minutes；
S13: adding 50ul 2M sulfuric acid to react to reacting hole with color development stopping, slightly shakes batten, and solution colour is turned by blue at this time
Become yellow, reads the absorbance in every hole respectively in 450nm and 540nm in 30 minutes.
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| CN201910801766.2A CN110426514B (en) | 2019-08-28 | 2019-08-28 | Method for detecting activity of peptide acyl arginine deiminase (PAD) |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62232398A (en) * | 1986-04-01 | 1987-10-12 | Amano Pharmaceut Co Ltd | Determination of enzymatic activity or substrate |
| CN1571842A (en) * | 2001-05-03 | 2005-01-26 | 阿克佐诺贝尔公司 | Peptidylarginine deiminase 6 |
| WO2007076302A2 (en) * | 2005-12-20 | 2007-07-05 | Boehringer Ingelheim International Gmbh | High throughput assay for modulators of peptidylarginine deiminase activity |
| CN102445545A (en) * | 2011-09-22 | 2012-05-09 | 广东现代农业集团研究院有限公司 | ELISA (Enzyme-Linked Immunosorbent Assay) quantitative detection kit fused with V5 label recombinant protein and ELISA quantitative detection method |
| US20120295292A1 (en) * | 2011-05-19 | 2012-11-22 | University Of South Carolina | Detecting Protein Arginine Deiminase (PAD) Activity in Human Tissues and Sera |
| US20130101611A1 (en) * | 2009-11-25 | 2013-04-25 | The Johns Hopkins University | Citrullination of human peptidylarginine deiminase 4 (pad-4) regulates its function and immunogenicity |
| CN103869086A (en) * | 2014-04-14 | 2014-06-18 | 杭州凯保罗生物科技有限公司 | Serum autoantibody detection kit |
| US20150376294A1 (en) * | 2012-12-03 | 2015-12-31 | Rigshospitalet | Anti-pad2 antibodies and treatment of autoimmune diseases |
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| WO2019112567A1 (en) * | 2017-12-05 | 2019-06-13 | The Scripps Research Institute | Methods and compositions related to selecting variant proteases |
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2019
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| JPS62232398A (en) * | 1986-04-01 | 1987-10-12 | Amano Pharmaceut Co Ltd | Determination of enzymatic activity or substrate |
| CN1571842A (en) * | 2001-05-03 | 2005-01-26 | 阿克佐诺贝尔公司 | Peptidylarginine deiminase 6 |
| WO2007076302A2 (en) * | 2005-12-20 | 2007-07-05 | Boehringer Ingelheim International Gmbh | High throughput assay for modulators of peptidylarginine deiminase activity |
| US20130101611A1 (en) * | 2009-11-25 | 2013-04-25 | The Johns Hopkins University | Citrullination of human peptidylarginine deiminase 4 (pad-4) regulates its function and immunogenicity |
| US20120295292A1 (en) * | 2011-05-19 | 2012-11-22 | University Of South Carolina | Detecting Protein Arginine Deiminase (PAD) Activity in Human Tissues and Sera |
| CN102445545A (en) * | 2011-09-22 | 2012-05-09 | 广东现代农业集团研究院有限公司 | ELISA (Enzyme-Linked Immunosorbent Assay) quantitative detection kit fused with V5 label recombinant protein and ELISA quantitative detection method |
| US20150376294A1 (en) * | 2012-12-03 | 2015-12-31 | Rigshospitalet | Anti-pad2 antibodies and treatment of autoimmune diseases |
| CN103869086A (en) * | 2014-04-14 | 2014-06-18 | 杭州凯保罗生物科技有限公司 | Serum autoantibody detection kit |
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| DRES DAMGAARD等: "Demonstration of extracellular peptidylarginine deiminase (PAD) activity in synovial fluid of patients with rheumatoid arthritis using a novel assay for citrullination of fibrinogen", 《ARTHRITIS RESEARCH & THERAPY》 * |
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