CN110426258A - A kind of glass slide and its preparation process with cell adherence ability - Google Patents
A kind of glass slide and its preparation process with cell adherence ability Download PDFInfo
- Publication number
- CN110426258A CN110426258A CN201910742498.1A CN201910742498A CN110426258A CN 110426258 A CN110426258 A CN 110426258A CN 201910742498 A CN201910742498 A CN 201910742498A CN 110426258 A CN110426258 A CN 110426258A
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- Prior art keywords
- glass slide
- polymethacrylamide
- aminopropyl
- coatings
- reagent
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of glass slide and its preparation process with cell adherence ability, including glass slide, coatings of reagent is coated in the upper surface of glass slide, coatings of reagent is polymethacrylamide and the silane coupling agent containing amino, wherein, the molecular weight of polymethacrylamide is 2,000,000-800 ten thousand, amino silane can be 3- aminopropyl-trimethyl silane or 3- aminopropyl-triethoxysilane, when preparing glass slide, configure certain density polymethacrylamide and 3- aminopropyl-trimethoxy silane aqueous solution, clean glass slide is put and is impregnated 5 minutes in the above solution, take out glass slide, it is rinsed with water, remove the loose adsorption layer of slide surface, by glass slide as in baking oven, 60 degree of dryings, this method takes water as a solvent, without any organic solvent, with green ring The advantages of guarantor, the glass slide of preparation can be realized cell in the adherency on its surface, can be to avoid in pathological examination the phenomenon that cell detachment.
Description
Technical field
The present invention relates to the fabricating technology field of glass slide, specially a kind of glass slide with cell adherence ability
And its preparation process.
Background technique
Adhere to glass slide has wide application demand in terms of Clinicopathologic Diagnosis, universities and colleges；Mesh
Before, adherency glass slide mainly constructs the coating with positive charge using in surface of glass slide, and most commonly polylysine applies
The problem of layer and amino silane coating, these methods are primarily present is: polylysine price is very expensive, and difficulty or ease are realized real
Large-scale production；Product containing amino silane is not easy long-term preservation, and properties of product are unstable；In addition, it is reported in the literature containing
The organic solvents such as acetone, ethyl alcohol are often used in the glass slide preparation method of amino silane, there are environmental issues.
Summary of the invention
The purpose of the present invention is to provide a kind of glass slide and its preparation process with cell adherence ability, on solving
State the problem of proposing in background technique.
To achieve the above object, the invention provides the following technical scheme: a kind of glass slide with cell adherence ability, packet
Glass slide is included, the glass slide is equipped with coatings of reagent, contains polymethacrylamide in coatings of reagent, the silane containing amino
Coupling agent.
Preferably, the molecular weight of the polymethacrylamide in the coatings of reagent is 2,000,000-800 ten thousand.
Preferably, the amino silane in the coatings of reagent is 3- aminopropyl-trimethyl silane, 3- aminopropyl-three
Ethoxysilane.
Preferably, a kind of glass slide with cell adherence ability, preparation process the following steps are included:
A, certain density polymethacrylamide and 3- aminopropyl-trimethoxy silane aqueous solution are configured；
B, clean glass slide is put and is impregnated 5 minutes in the above solution；
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface；
D, by glass slide as in baking oven, 60 degree of dryings.
Compared with prior art, the beneficial effects of the present invention are:
(1) this method takes water as a solvent, and is free of any organic solvent, has the characteristics that environmentally protective；
(2) polymethacrylamide and amino silane used is cheap, is mass produced convenient for enterprise；
(3) method impregnated using solution, it is easy to operate；
(4) glass slide prepared by can be realized cell in the adherency on its surface, can be to avoid the cell detachment in pathological examination
The phenomenon that.
Detailed description of the invention
Fig. 1 is slide configurations schematic diagram of the present invention.
In figure: 1, glass slide；2, coatings of reagent.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1 is please referred to, the present invention provides a kind of technical solution: a kind of glass slide with cell adherence ability, including carry
Slide 1, glass slide 1 are equipped with coatings of reagent 2, contain polymethacrylamide in coatings of reagent 2, contain the silane coupled of amino
Agent, the molecular weight of the polymethacrylamide in coatings of reagent 2 are 2,000,000-800 ten thousand, and the amino silane in coatings of reagent 2 is 3-
Aminopropyl-trimethyl silane, 3- aminopropyl-triethoxysilane.
A kind of glass slide with cell adherence ability, preparation process the following steps are included:
A, certain density polymethacrylamide and 3- aminopropyl-trimethoxy silane aqueous solution are configured；
B, clean glass slide is put and is impregnated 5 minutes in the above solution；
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface；
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 1, A, 0.1% polymethacrylamide of configuration and 0.1% 3- aminopropyl-trimethoxy silane are water-soluble
Liquid.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 2, A, 0.5% polymethacrylamide of configuration and 1% 3- aminopropyl-trimethoxy silane aqueous solution.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 3, A, 1% polymethacrylamide of configuration and 3% 3- aminopropyl-trimethoxy silane aqueous solution.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 4, A, 0.1% polymethacrylamide of configuration and 0.1% 3- aminopropyl-triethoxysilane are water-soluble
Liquid.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 5, A, 0.5% polymethacrylamide of configuration and 1% 3- aminopropyl-triethoxysilane aqueous solution.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
Embodiment 6, A, 1% polymethacrylamide of configuration and 3% 3- aminopropyl-triethoxysilane aqueous solution.
B, clean glass slide is put and is impregnated 5 minutes in the above solution.
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface.
D, by glass slide as in baking oven, 60 degree of dryings.
This method takes water as a solvent, and is free of any organic solvent, has the advantages that environmentally protective, the glass slide of preparation can
Realize that cell, can be to avoid in pathological examination the phenomenon that cell detachment in the adherency on its surface.
The beneficial effects of the present invention are:
(1) this method takes water as a solvent, and is free of any organic solvent, has the characteristics that environmentally protective；
(2) polymethacrylamide and amino silane used is cheap, is mass produced convenient for enterprise；
(3) method impregnated using solution, it is easy to operate；
(4) glass slide prepared by can be realized cell in the adherency on its surface, can be to avoid the cell detachment in pathological examination
The phenomenon that.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of glass slide with cell adherence ability, including glass slide (1), it is characterised in that: set on the glass slide (1)
Have coatings of reagent (2), coatings of reagent contains polymethacrylamide in (2), the silane coupling agent containing amino.
2. a kind of glass slide with cell adherence ability according to claim 1, it is characterised in that: the coatings of reagent
(2) molecular weight of the polymethacrylamide in is 2,000,000-800 ten thousand.
3. a kind of glass slide with cell adherence ability according to claim 1, it is characterised in that: the coatings of reagent
(2) amino silane in is 3- aminopropyl-trimethyl silane, 3- aminopropyl-triethoxysilane.
4. realize a kind of glass slide with cell adherence ability described in claim 1, preparation process the following steps are included:
A, certain density polymethacrylamide and 3- aminopropyl-trimethoxy silane aqueous solution are configured；
B, clean glass slide is put and is impregnated 5 minutes in the above solution；
C, glass slide is taken out, is rinsed with water, the loose adsorption layer of removal slide surface；
D, by glass slide as in baking oven, 60 degree of dryings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910742498.1A CN110426258A (en) | 2019-08-13 | 2019-08-13 | A kind of glass slide and its preparation process with cell adherence ability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910742498.1A CN110426258A (en) | 2019-08-13 | 2019-08-13 | A kind of glass slide and its preparation process with cell adherence ability |
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| Publication Number | Publication Date |
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| CN110426258A true CN110426258A (en) | 2019-11-08 |
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| CN201910742498.1A Pending CN110426258A (en) | 2019-08-13 | 2019-08-13 | A kind of glass slide and its preparation process with cell adherence ability |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112945671A (en) * | 2021-02-07 | 2021-06-11 | 南昌大学附属口腔医院(江西省口腔医院) | Adhesive glass slide and preparation method and application thereof |
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|---|---|---|---|---|
| US20050106721A1 (en) * | 2002-03-01 | 2005-05-19 | Teruyuki Nagamune | Immobilized cells and liposomes and method of immobilizing the same |
| CN101048663A (en) * | 2004-09-23 | 2007-10-03 | 三路影像公司 | Polycationic polymer coatings for immobilizing biological samples |
| CN101824266A (en) * | 2010-05-05 | 2010-09-08 | 南通亿诺高分子新型材料有限公司 | Coating material for experiment glass slides and preparation method thereof |
| CN107063812A (en) * | 2017-03-30 | 2017-08-18 | 南通大学 | A kind of low flake rate of liquid-based culture hydrophily adheres to the preparation method of slide |
| CN108033690A (en) * | 2017-12-10 | 2018-05-15 | 盐城市耀华玻璃仪器厂 | One kind adhesion glass slide and its production technology |
-
2019
- 2019-08-13 CN CN201910742498.1A patent/CN110426258A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050106721A1 (en) * | 2002-03-01 | 2005-05-19 | Teruyuki Nagamune | Immobilized cells and liposomes and method of immobilizing the same |
| CN101048663A (en) * | 2004-09-23 | 2007-10-03 | 三路影像公司 | Polycationic polymer coatings for immobilizing biological samples |
| CN101824266A (en) * | 2010-05-05 | 2010-09-08 | 南通亿诺高分子新型材料有限公司 | Coating material for experiment glass slides and preparation method thereof |
| CN107063812A (en) * | 2017-03-30 | 2017-08-18 | 南通大学 | A kind of low flake rate of liquid-based culture hydrophily adheres to the preparation method of slide |
| CN108033690A (en) * | 2017-12-10 | 2018-05-15 | 盐城市耀华玻璃仪器厂 | One kind adhesion glass slide and its production technology |
Non-Patent Citations (6)
| Title |
|---|
| DARWIN R. REYES 等: "Micropatterning Neuronal Cells on Polyelectrolyte Multilayers", 《LANGMUIR》 * |
| MADDOX, P H 等: "3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion", 《JOURNAL OF CLINICAL PATHOLOGY》 * |
| MANEESH JAISWAL 等: "Cell adhesion and proliferation studies on semi-interpenetrating polymeric networks (semi-IPNs) of polyacrylamide and gelatin", 《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS》 * |
| 张娜: "N-羟甲基丙烯酰胺聚合物刷的制备及其性能研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
| 徐开先 等: "《传感器实用技术》", 31 December 2016, 国防工业出版社 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112945671A (en) * | 2021-02-07 | 2021-06-11 | 南昌大学附属口腔医院(江西省口腔医院) | Adhesive glass slide and preparation method and application thereof |
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