CN110423722A - A kind of culture medium and its application and induction method of the tendon stem cell to bone cell differentiation - Google Patents
A kind of culture medium and its application and induction method of the tendon stem cell to bone cell differentiation Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/133—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from tenocytes
Abstract
The present invention relates to stem cells technology field more particularly to a kind of culture medium and its applications and induction method of the tendon stem cell to bone cell differentiation.FBS, vitamin C, dexamethasone, sodium β-glycerophosphate and BMP-2 are added into basal medium by the present invention, the induction broth as tendon stem cell Osteoblast Differentiation.Tendon stem cell is improved to the quantity of bone cell differentiation and shortens the time of differentiation.Experiment shows to induce tendon stem cell with culture solution provided by the invention can occur the characteristic of osteocyte in 21, cellular morphology changes and Alizarin red staining cell number increases.After induction 28 days, cellular morphology is gradually changed, and in polygonal, the calcium scoring deposited in cytoplasm containing rock salt, Alizarin red staining is positive.
Description
Technical field
The present invention relates to stem cells technology field more particularly to a kind of culture medium and its application and induction tendon stem cell to
The method of bone cell differentiation.
Background technique
Tendinopathy is common in the crowd of high amount of exercise, common using local pain, swelling, dysfunction as Clinical symptoms
Site of pathological change is rotator cuff, supraspinatus, kneecap tendon and heel string.The pathogenesis of tendinopathy is not yet studied clear completely at present, to tendon
Disease treatment method it is limited, curative effect is indefinite, this with to the disease pathomechanism lack understand in depth it is related.
American scholar in 2007 isolates a kind of cell with self-renewal capacity from the tendon of people and rabbit, is ordered
Entitled tendon stem cell or progenitor cells (tendon-derived stem cells, TDSCs).Hong Kong scholar in 2010 is from mouse flesh
Also tendon stem cell is isolated in tendon.The cell has multi-lineage potential, and different under the effect of exogenous prostaglandin E2
Often differentiation.
Currently, thering is scholar to propose that the abnormal differentiation of tendon stem cell may be the pathogenesis basis of tendinopathy, but at present to flesh
The differentiation mechanism of tendon stem cell is not yet studied clear.In order to understand chronic tendon diseased tissues Pathologic changes provide cell biology according to
According to also the clinical treatment for chronic tendon disease provides new approaches, should further study the differentiation mechanism of tendon stem cell, develops flesh
Method of the tendon stem cell to bone cell differentiation.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is that providing a kind of culture medium and its application and induction tendon is dry
For cell to the method for bone cell differentiation, culture medium provided by the invention can promote tendon stem cell to bone cell differentiation, point
The change period is short, and differentiation efficiency is high.
Culture medium provided by the invention include basal medium, FBS, vitamin C, dexamethasone, sodium β-glycerophosphate and
BMP-2。
Bone morphogenesis protein-2 (bone morphogenetic protein-2, BMP-2) is that transforming growth factor β is super
One of family member has and induces undifferentiated mescenchymal stem cell to chondroblast and osteoblast directed differentiation and proliferation energy
Power promotes osteoblast differentiation mature, participates in bone and cartilage-derived growth development and its reconstruction process, and then accelerate bone defect healing.
In the embodiment of the present invention, the concentration of BMP-2 is 100ng/mL~900ng/mL.In the embodiment of the present invention, the concentration of BMP-2 is
100ng/mL, 300ng/mL, 500ng/mL, 700ng/mL or 900ng/mL.
Vitamin C, dexamethasone, sodium β-glycerophosphate are common in current stem cell osteogenic induction or chondrocyte induction liquid
Component, wherein dexamethasone by promote Cbfal mRNA and Osterix mRNA expression promote marrow stromal cell to
Osteoblast differentiation.Sodium β-glycerophosphate can provide phosphate ion for osteoblast, while promote the deposition of physiological calcium salt
And calcification, it is the necessary condition that bone marrow stroma stem cell generates Mineral nodules.Vitamin C (ascorbic acid) can increase calcium in vitro
The deposition of salt promotes the formation of Mineral nodules, and adjusts the differentiation of osteoclast indirectly by mesenchymal cell.The present invention is implemented
In example, ascorbic concentration is 10mg/L；The concentration of dexamethasone is 100nmol/L；The concentration of sodium β-glycerophosphate is
20mmol/L。
Fetal calf serum (fetal calf serum, abbreviation FBS) is one of serum, is capable of providing maintenance cell index
The hormone of growth does not have or measures seldom nutrients and main low molecule nutrients in basal medium.The present invention is implemented
In example, the volume fraction of FBS is 10%.
DMEM is a kind of culture medium containing various amino acid and glucose, is developed on the basis of MEM culture medium.With
MEM relatively increases various composition dosage, while being divided into high glycoform (lower than 4500mg/L) and low-sugar type again (lower than 1000mg/
L).Basic culture solution used in the present invention can be that self-control is also that purchase obtains, and which is not limited by the present invention, and implementation all exists
Within protection scope of the present invention.In the embodiment of the present invention, basal medium is L-DMEM culture solution.That is low-sugar type DMEM culture
Liquid, wherein the concentration of glucose is 1000mg/L.
In one specific embodiment, culture medium includes:
In one specific embodiment, culture medium includes:
In one specific embodiment, culture medium includes:
In one specific embodiment, culture medium includes:
In one specific embodiment, culture medium includes:
Application of the culture medium provided by the invention in induction tendon stem cell into bone cell differentiation.
In the present invention, tendon stem cell is people's heel string source tendon stem cell.
The present invention also provides a kind of induction method of the tendon stem cell to bone cell differentiation, with culture provided by the invention
Base induces tendon stem cell.
In method provided by the invention, tendon stem cell is people's heel string source tendon stem cell, isolated culture method packet
It includes:
Step 1: heel string tissue being cut into 1mm × 1mm × 1mm fragment, is 37 DEG C of collagenase type I of 3mg/mL with concentration
After digesting 2h, filter to form single cell suspension through 70 μm of filters；
Step 2: single cell suspension being centrifuged 5min with 300 × g, by cell precipitation with 500/cm2Cell density inoculation
After cultivating 10d to complete medium (the L-DMEM culture medium containing 10v%FBS, 100U/mL penicillin, 100mg/mL streptomysin),
After mass fraction is 0.25% trypsin digestion, it is labeled as primary cell, is passed on complete medium.
The tendon stem cell that the present invention is induced is the 3rd generation tendon stem cell；Induced density is 5.0 × 103cells/
cm2。
Every 3 days replacement culture mediums are induced in the present invention；The time of the induction is 4 weeks.
FBS, vitamin C, dexamethasone, sodium β-glycerophosphate and BMP-2 are added into basal medium by the present invention, as
The induction broth of tendon stem cell Osteoblast Differentiation.Tendon stem cell is improved to the quantity of bone cell differentiation and shortens differentiation
Time.Experiment shows to induce tendon stem cell with culture solution provided by the invention can occur the spy of osteocyte in 21 days
Property, cellular morphology changes and Alizarin red staining cell number increases.After induction 28 days, cellular morphology is gradually changed, in more
Angular, the calcium scoring containing rock salt deposition in cytoplasm, Alizarin red staining is positive.
Detailed description of the invention
Fig. 1 shows the case where Alizarin red staining of group of cells.
Specific embodiment
The present invention provides a kind of culture medium and its application and induction method of the tendon stem cell to bone cell differentiation, abilities
Field technique personnel can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that all similar replaces
Change and change apparent to those skilled in the art, they are considered as being included in the present invention.Side of the invention
Method and application be described by preferred embodiment, related personnel obviously can not depart from the content of present invention, spirit and
To methods herein and application is modified or appropriate changes and combinations in range, carry out implementation and application the technology of the present invention.
The test material that the present invention uses is all common commercially available product, can all be bought in market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
Take people with tendinous tissue under aseptic condition, PBS is rinsed 3 times, wipes out surface portion connective tissue, is separated with tendinous tissue.
Heel string is cut into 1mm × 1mm × 1mm fragment, 37 DEG C of digestion 2h of 3mg/mL Type I collagen enzyme, 70 μm of filters filter to be formed it is slender
Born of the same parents' suspension.By single cell suspension with 300 × g be centrifuged 5min, abandon supernatant, by cell precipitation complete medium (containing 10%FBS,
The L-DMEM culture medium of 100U/mL penicillin, 100mg/mL streptomysin) in be resuspended, with 500/cm2Cell density is seeded to directly
In diameter 10cm culture dish, 10d is cultivated, is mixed after 0.25% trypsin digestion, primary cell is labeled as.Cell is paved with bottom of bottle and reaches
It is passed on after 90%, abandons culture solution, sterile PBS is cleaned 2 times, 0.25% trypsin digestion 3min, and complete medium is added and terminates
Digestion；Cell suspension is centrifuged 5min with 180g, abandons supernatant, 2mL complete medium is added, cell is resuspended, and counts cell and presses 5 × 103
A/cm2Cell density is seeded to Tissue Culture Flask.Take the 1st~3 generation cell for subsequent experimental.Inverted phase contrast microscope observation
Cellular morphology variation.
The karyocyte obtained from heel string tissue digestion is with 500/cm2After density inoculation, cell is in cloning growth.It is former
The visible multiple tufted cell colonies for being dispersed in distribution of the feeding 10d that is commissioned to train, in from center to external radiation sample adherent growth, cellular morphology is not
Rule, based on polygonal, nucleus is gathered in center, and the protrusion that cytoplasm is formed is radial outward.
The 3rd generation cell is taken, is washed 2 times with sterile PBS, 0.25% trypsin digestion, at room temperature with 350 × g centrifugation
Cell precipitation is resuspended in dye solution, 4 DEG C of placement 15min by 5min.Every 100 microlitres of cell suspensions are sequentially added into algae
CD44, CD90, CD34, CD105 primary antibody of red eggs white marker, the CD45 primary antibody of FITC label, CD146 primary antibody；Every pipe sample simultaneously
Product set up homotype negative control, 4 DEG C of incubation 15min.After washing cell 3 times with the pre-cooling PBS containing 2% bovine serum albumin(BSA), stream
The detection of formula cell instrument.The result shows that MSCs specific surfaces mark CD44, CD90 and CD105 positive rate of hATDSCs is respectively
99.75% ± 0.21%, 99.84% ± 0.12% and 95.76% ± 0.64%；It is hemopoietic stem cell surface mark CD34, white thin
The positive expression rate of cellular surface mark CD45 and endothelial cell surface mark CD146 is respectively 3.34% ± 0.36%,
4.72% ± 0.24% and 4.37% ± 0.17%.
Embodiment 2
1), culture medium is prepared according to table 1:
1 culture medium prescription of table
2) the 3rd generation cell is taken, with 5 × 104A/hole density is inoculated in 6 orifice plates, is divided into osteogenic induction group and control group.Its
In, osteogenic induction group is using the culture medium of group 1~6 in table 1, and control group is using complete medium.Osteogenic induction group attached cell
Osteogenic Induction Medium 2mL is replaced after growth fusion, control group continues with complete medium culture 2mL culture.The training of replacement in every 3 days
Base is supported, until 4 Zhou Shihang Alizarin red stainings are identified.
3) it, cultivates 20 days, the cellular morphology of group 1~5 gradually changes, and is in polygonal, deposits in cytoplasm containing rock salt
Calcium scoring, Alizarin red staining is positive；Culture 28 days, 6 cellular morphologies of group gradually change, and are in polygonal, in cytoplasm
Calcium scoring containing rock salt deposition, Alizarin red staining are positive；Complete medium control group is feminine gender.
The number of days of osteogenic induction is counted as the standard of cell differentiation osteoblast by Alizarin red staining using success.And root
According to the area for counting Mineral nodules the case where Alizarin red staining, such as table 2, Fig. 1:
Table 2 induces result
| Grouping | Osteogenic induction number of days | Mineralising area % |
| Control | ∞ | 0 |
| Group 1 | 26 days | 30% |
| Group 2 | 25 days | 35% |
| Group 3 | 20 days | 75% |
| Group 4 | 20 days | 80% |
| Group 5 | 20 days | 75% |
| Group 6 | 28 days | 15% |
| Group 7 | 21 days | 15% |
The results show that group 1~7 provide culture medium all can effectively Cell differentiation inducing activity become osteocyte, but relative to
Group 6~7, the culture medium of group 1~5 can shorten osteogenic induction number of days, increase mineralising area.And wherein, the culture medium of group 3~5
Induction number of days can more significantly be shortened relative to other culture mediums and increase mineralising area, through analyzing, which has statistics
Learn difference, p < 0.05.
4), alkaline phosphatase activities (ALP) measure
Break up culture 0d, 7d, 14d and 21d respectively at induction to each group MDSCs to determine cell progress alkaline phosphatase activities
It is fixed to measure.Specific measuring method are as follows:
Original fluid is abandoned, PBS washes 3 times, and every hole adds 200 μ L 0.2%TritonX-100,37 DEG C of incubation 1h, visible under mirror
Cell cracking is complete, and lysate is sucked out to new centrifuge tube, takes supernatant after centrifugation, gives over to spare.
Determining the protein quantity is carried out to above-mentioned lysate, is carried out in 96 orifice plates.It is said by BCA determination of protein concentration kit
Bright book requires setting measurement hole, gauge orifice and blank well, and every group sets 3 multiple holes, after every boreliquid 20 μ L, 37 DEG C of incubation 30min
Detect 562nmOD value, blank well zeroing.Protein concentration standard curve is drawn according to gauge orifice OD value, substitutes into each hole OD value, is calculated
The protein concentration of each group.
Determination of alkaline phosphatase activity is carried out to above-mentioned lysate, is carried out in 96 orifice plates.It is tried by alkaline phosphatase assay
Agent box specification requires setting measurement hole, gauge orifice and blank well, and 37 DEG C of incubation 15min, every group sets 3 multiple holes, and every hole adds 150
μ L developing solution gently shakes orifice plate and mixes.Detect 520nmOD value, blank well zeroing.Alkaline phosphatase is calculated according to the following formula
Activity:
Alkaline phosphatase activities (U/gprot)=(measurement hole absorbance-blank well absorbance) ÷ (gauge orifice absorbance-
Blank well absorbance) × phenol standard concentration ÷ sample to be tested protein concentration (gprot/mL).
Table 3 ALP activity
| Number of days-d | 0d | 7d | 14d | 21d |
| Control | 10.5±2.1 | 562.2±56.6 | 988.6±103.5 | 1253.2±157.4 |
| Group 3 | 10.4±2.0 | 994.3±135.2 | 1665.5±142.2 | 2756.3±136.2 |
| Group 6 | 10.3±2.2 | 655.5±111.7 | 1224.5±155.4 | 1736.2±152.2 |
| Group 7 | 10.1±1.8 | 481.3±88.5 | 985.5±140.2 | 1533.2±142.2 |
The results show that cultivating the 7th, 14,21 day relative to control group, the ALP activity of group 3,6,7, which all exists with control group, unites
Meter learns difference；Wherein, 3 ALP activity highest is organized, conspicuousness is higher than group 6~7, p < 0.05.
The above is only the preferred embodiment of the present invention, it is noted that those skilled in the art are come
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of culture medium, which is characterized in that by basal medium, FBS, vitamin C, dexamethasone, sodium β-glycerophosphate and
BMP-2 composition.
2. culture medium according to claim 1, which is characterized in that the concentration of the BMP-2 is 100ng/mL~900ng/
mL。
3. culture medium according to claim 1, which is characterized in that the ascorbic concentration is 10mg/L；Dexamethasone
Concentration be 100nmol/L；The concentration of sodium β-glycerophosphate is 20mmol/L.
4. culture medium according to claim 1, which is characterized in that the volume fraction of the FBS is 10%.
5. culture medium according to claim 1, which is characterized in that the basal medium is L-DMEM culture solution.
6. application of the described in any item culture mediums of Claims 1 to 5 in induction tendon stem cell into bone cell differentiation.
7. application according to claim 1, which is characterized in that the tendon stem cell is that people's heel string source tendon is dry thin
Born of the same parents.
8. a kind of induction method of the tendon stem cell to bone cell differentiation, which is characterized in that with any one of Claims 1 to 5 institute
The culture medium induction tendon stem cell stated.
9. the method according to claim 1, wherein the tendon stem cell is the 3rd generation tendon stem cell；Induction
Density is 5.0 × 103cells/cm2。
10. the method according to claim 1, wherein every 3 days replacement culture mediums of the induction；The induction
Time is 4 weeks.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008891A (en) * | 2021-03-22 | 2021-06-22 | 南京鼓楼医院 | Construction method and application for screening high-quality osteogenic differentiation potential mesenchymal stem cell quantification standard |
| CN113633606A (en) * | 2021-08-16 | 2021-11-12 | 南京鼓楼医院 | Preparation method and application of nano-motor-driven exosome-loaded microneedle special for treating end diseases |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102041245A (en) * | 2010-11-26 | 2011-05-04 | 中国人民解放军总医院 | Kit for inducing differentiation from bone mesenchymal stem cells to osteoblasts, application of kit and method for inducing cell differentiation |
| US20140178346A1 (en) * | 2012-12-26 | 2014-06-26 | The Regents Of The University Of California | Cellular compositions for tissue engineering |
| CN105211053A (en) * | 2015-10-29 | 2016-01-06 | 广州赛莱拉干细胞科技股份有限公司 | Cryopreservation solution for tendon stem cells derived from human achilles tendon and preparation method thereof |
| CN105802906A (en) * | 2016-05-27 | 2016-07-27 | 中国医学科学院医学生物学研究所 | Culture medium for osteogenic induced differentiation of mesenchymal stem cells of tupaia belangeri and preparation method thereof and application |
| CN106434539A (en) * | 2016-09-30 | 2017-02-22 | 广州赛莱拉干细胞科技股份有限公司 | Osteogenic induction medium and osteogenic differentiation method |
-
2019
- 2019-09-03 CN CN201910827325.XA patent/CN110423722A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102041245A (en) * | 2010-11-26 | 2011-05-04 | 中国人民解放军总医院 | Kit for inducing differentiation from bone mesenchymal stem cells to osteoblasts, application of kit and method for inducing cell differentiation |
| US20140178346A1 (en) * | 2012-12-26 | 2014-06-26 | The Regents Of The University Of California | Cellular compositions for tissue engineering |
| CN105211053A (en) * | 2015-10-29 | 2016-01-06 | 广州赛莱拉干细胞科技股份有限公司 | Cryopreservation solution for tendon stem cells derived from human achilles tendon and preparation method thereof |
| CN105802906A (en) * | 2016-05-27 | 2016-07-27 | 中国医学科学院医学生物学研究所 | Culture medium for osteogenic induced differentiation of mesenchymal stem cells of tupaia belangeri and preparation method thereof and application |
| CN106434539A (en) * | 2016-09-30 | 2017-02-22 | 广州赛莱拉干细胞科技股份有限公司 | Osteogenic induction medium and osteogenic differentiation method |
Non-Patent Citations (6)
| Title |
|---|
| YUN FENG RUI ET AL.: ""BMP-2 stimulated non-tenogenic differentiation and promoted proteoglycan deposition of tendon-derived stem cells(TDSCs)in vitro"", 《JOURNAL OF ORTHOPAEDIC RESEARCH》 * |
| YUN FENG RUI ET AL.: ""Higher BMP receptor expression and BMP-2-inducedosteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells"", 《INTERNATIONAL ORTHOPAEDICS》 * |
| 刘立峰等: "《现代骨科诊疗学》", 30 June 2009, 内蒙古科学技术技术出版社 * |
| 匡威等: ""不同年龄小鼠骨髓间充质干细胞中miR203 的差异及其对细胞增殖的调控"", 《中国骨质疏松杂志》 * |
| 林禹丞等: ""骨形态发生蛋白2诱导慢性腱病大鼠肌腱干细胞体外成骨、成软骨分化"", 《中国组织工程研究》 * |
| 芮云峰等: ""BMP-2诱导人跟腱来源肌腱干细胞成软骨分化的实验研究"", 《干细胞与组织工程》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008891A (en) * | 2021-03-22 | 2021-06-22 | 南京鼓楼医院 | Construction method and application for screening high-quality osteogenic differentiation potential mesenchymal stem cell quantification standard |
| CN113633606A (en) * | 2021-08-16 | 2021-11-12 | 南京鼓楼医院 | Preparation method and application of nano-motor-driven exosome-loaded microneedle special for treating end diseases |
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