CN110420164A - A kind of snail essence dry powder and the preparation method and application thereof - Google Patents
A kind of snail essence dry powder and the preparation method and application thereof Download PDFInfo
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- CN110420164A CN110420164A CN201910817338.9A CN201910817338A CN110420164A CN 110420164 A CN110420164 A CN 110420164A CN 201910817338 A CN201910817338 A CN 201910817338A CN 110420164 A CN110420164 A CN 110420164A
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- essence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
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- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of snail essence dry powder, is made of following steps: (1) being homogenized snail mucus addition pure water to obtain homogenate one, centrifugation is added ethyl alcohol and Essence is precipitated;(2) Essence is centrifuged to obtain precipitating one, homogenate obtains homogenate two after being dissolved in ultrapure water, and suction filtration obtains filtrate, is concentrated under reduced pressure to give concentrate;(3) concentrate is centrifuged to obtain supernatant one, dehydrated alcohol is added, filtered after standing overnight and obtain snail essence crude extract;(4) pure water is added in snail essence crude extract, stirring centrifugation to after being completely dissolved obtains supernatant two, and dialysis 48h obtains Essence;(5) it is freeze-dried, mills after taking-up up to snail essence dry powder after Essence being concentrated under reduced pressure.The invention also discloses the preparation methods and application of the snail essence dry powder.Snail essence dry powder nature provided by the invention is stablized, and has the effect of preferably promoting skin regeneration, desalination scar spot, and the addition convenient for storage and different cosmetics uses.
Description
Technical field
The present invention relates to a kind of snail essence dry powder and the preparation method and application thereof.
Background technique
Snail mucus is the mucus that snail secretes when creeping, it can effectively avoid skin aging, and can eliminate on skin
Spot, scar and acne.It is that pole has protective effect to skin using the cold cream that the regeneration effect of snail makes.Snail point
Natural allantoin, calcium, Glycolic acid, ossein, protein and vitamin contained by the mucus secreted etc. are that production cosmetics must not
The material that can lack.
As in recent years in star's product of cosmetic skin care conduct industry the supreme arrogance of a person with great power --- snail mucus is concerned.Snail
Ox mucus has the function of unique regeneration, stimulating cellular growth, easing pain and diminishing inflammation, has been widely used medical skin care conduct
Industry.However allantoin contained in snail is contained in snail mucus, and snail mucus not only collects inconvenience, is less useful for protecting
It deposits, problem above greatly constrains the development and utilization of snail Related product.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of snail essence dry powder, property is stablized, and has preferable promote
Into skin regeneration, desalinate the effect of scar spot, and storage and the addition of different cosmetics is convenient for use.
In order to solve the above technical problems, the technical scheme is that
A kind of snail essence dry powder, is made of following steps:
(1) it is homogenized the pure water that 1-3 times of weight is added in snail mucus to obtain homogenate one, homogenate one is centrifuged 5-15min
Essence is precipitated in the ethyl alcohol that 3 times of volumes are added after removal insoluble impurities;
(2) the Essence centrifugation 5-15min that step (1) obtains is obtained into precipitating one, is homogenized after precipitating one is dissolved in ultrapure water
0.5-2h obtains homogenate two, the suction filtration of homogenate two is obtained filtrate, the 1/5-1/2 for concentrating filtrate to original volume is obtained
Concentrate;
(3) the concentrate centrifugation 3-20min that step (2) obtains is obtained into supernatant one, dehydrated alcohol is added into supernatant one
Filtering until the concentration of ethyl alcohol is 60-85%, after standing overnight and obtaining precipitating two is snail essence crude extract;
(4) the snail essence crude extract for obtaining step (3) is added in pure water, and stirring is centrifuged 5-10min to after being completely dissolved
Removal insoluble impurities obtains supernatant two, and the bag filter dialysis 48h that supernatant two is 3500 with molecular cut off is obtained essence
Magnificent liquid;
(5) 1/5-1/2 that the Essence process that step (4) obtains is concentrated under reduced pressure into original volume freezing in freeze dryer is placed on to do
Dry, powdering of milling after taking-up is up to snail essence dry powder.
Preferably, in the step (1), the centrifugal speed of homogenate one is 3000-5000r/min.
Preferably, in the step (2), the centrifugal speed of Essence is 3000-5000r/min, precipitating one and ultrapure water
Weight ratio be 1:1-3.
Preferably, in the step (3), the centrifugal speed of concentrate is 3000-5000r/min.
Preferably, in the step (4), the weight ratio of snail essence crude extract and pure water is 1:1-3, the speed of centrifugation
For 3000-5000r/min.
Another technical problem to be solved by the present invention is that providing the preparation method of above-mentioned snail essence dry powder.
In order to solve the above technical problems, technical solution is:
A kind of preparation method of snail essence dry powder, comprising the following steps:
(1) it will be cleaned up after snail decladding, the pure water that 1-3 times of weight is added is homogenized to obtain homogenate one, by homogenate one
It is centrifuged the ethyl alcohol precipitation Essence that 5-15min removes 3 times of volumes of addition after insoluble impurities;
(2) the Essence centrifugation 5-15min that step (1) obtains is obtained into precipitating one, is homogenized after precipitating one is dissolved in ultrapure water
0.5-2h obtains homogenate two, the suction filtration of homogenate two is obtained filtrate, the 1/5-1/2 for concentrating filtrate to original volume is obtained
Concentrate;
(3) the concentrate centrifugation 3-20min that step (2) obtains is obtained into supernatant one, dehydrated alcohol is added into supernatant one
Filtering until the concentration of ethyl alcohol is 60-85%, after standing overnight and obtaining precipitating two is snail essence crude extract;
(4) the snail essence crude extract for obtaining step (3) is added in pure water, and stirring is centrifuged 5-10min to after being completely dissolved
Removal insoluble impurities obtains supernatant two, and the bag filter dialysis 48h that supernatant two is 3500 with molecular cut off is obtained essence
Magnificent liquid;
(5) 1/5-1/2 that the Essence process that step (4) obtains is concentrated under reduced pressure into original volume freezing in freeze dryer is placed on to do
Dry, powdering of milling after taking-up is up to snail essence dry powder.
The present invention also provides above-mentioned snail essence dry powder in moisturizing essence, nti-freckle Essence face cream or cleawhite over-night essences
Application in facial mask.
Compared with prior art, the invention has the following advantages:
1. superior biocompatibility: the present invention is derived from natural material, not will cause damage when contacting with human tissue organ,
It will not cause to be immunoreacted.
2. tissue regeneration ability is strong: the adherency of cell can be promoted, the growing environment of adaptation is provided for cell, accelerate skin
Regeneration.
3. antibacterial effect is brilliant: having the preferable natural ability for inhibiting antibacterial bacterium.
4. water-retaining property is strong: the moist environment of suitable skin growth can be provided, moreover it is possible to remove the exudate of additional pore.
5. snail essence dry powder provided by the invention is in dry powdered form, convenient for saving storage, can in varing proportions with basisization
Cosmetic carries out mixed preparing, so that the skin protection cosmetics of multifunctional and multipurpose be made.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes part of this application, not
Inappropriate limitation of the present invention is constituted, in the accompanying drawings:
Fig. 1 is that experimental example 1 promotes fibrocyte to increase the test result schematic diagram tested.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative examples and explanation of the invention
For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1
Snail essence dry powder, is made of following steps:
(1) it is homogenized the pure water that 2 times of weight are added in snail mucus to obtain homogenate one, one 4000r/min of homogenate is centrifuged
The ethyl alcohol precipitation Essence that 10min removes 3 times of volumes of addition after insoluble impurities is centrifuged under speed;
(2) Essence that step (1) obtains is centrifuged 10min under 4000r/min centrifugal speed and obtains precipitating one, it is molten by precipitating one
It is homogenized 1h after the ultrapure water of 2 times of weight and obtains homogenate two, the suction filtration of homogenate two is obtained into filtrate, is concentrated filtrate to
The 1/3 of original volume obtains concentrate;
(3) centrifugation 10min under concentrate 4000r/min centrifugal speed that step (2) obtains is obtained into supernatant one, to supernatant
Dehydrated alcohol is added in one until the concentration of ethyl alcohol is 70%, it is that snail essence are thick that suction filtration, which obtains precipitating two, after standing overnight
Extract;
(4) the snail essence crude extract for obtaining step (3) is added in the pure water of 2 times of weight, stirs to after being completely dissolved
It is centrifuged 8min removal insoluble impurities under 4000r/min centrifugal speed and obtains supernatant two, supernatant two is used into molecular cut off
Essence is obtained for 3500 bag filter dialysis 48h;
(5) Essence that step (4) obtains is placed on freeze-drying in freeze dryer by being concentrated under reduced pressure into the 1/3 of original volume,
Powdering is milled after taking-up up to snail essence dry powder.
Embodiment 2
Snail essence dry powder, is made of following steps:
(1) it is homogenized the pure water that 1 times of weight is added in snail mucus to obtain homogenate one, one 3000r/min of homogenate is centrifuged
The ethyl alcohol precipitation Essence that 15min removes 3 times of volumes of addition after insoluble impurities is centrifuged under speed;
(2) Essence that step (1) obtains is centrifuged 15min under 3000r/min centrifugal speed and obtains precipitating one, it is molten by precipitating one
It is homogenized 0.5h after the ultrapure water of 1 times of weight and obtains homogenate two, the suction filtration of homogenate two is obtained into filtrate, filtrate decompression is concentrated
1/2 to original volume obtains concentrate;
(3) centrifugation 20min under concentrate 3000r/min centrifugal speed that step (2) obtains is obtained into supernatant one, to supernatant
Dehydrated alcohol is added in one until the concentration of ethyl alcohol is 60%, it is that snail essence are thick that suction filtration, which obtains precipitating two, after standing overnight
Extract;
(4) the snail essence crude extract for obtaining step (3) is added in the pure water of 1 times of weight, stirs to after being completely dissolved
It is centrifuged 10min removal insoluble impurities under 3000r/min centrifugal speed and obtains supernatant two, supernatant two is used into molecular cut off
Essence is obtained for 3500 bag filter dialysis 48h;
(5) Essence that step (4) obtains is placed on freeze-drying in freeze dryer by being concentrated under reduced pressure into the 1/2 of original volume,
Powdering is milled after taking-up up to snail essence dry powder.
Embodiment 3
Snail essence dry powder, is made of following steps:
(1) it is homogenized the pure water that 3 times of weight are added in snail mucus to obtain homogenate one, one 5000r/min of homogenate is centrifuged
The ethyl alcohol precipitation Essence that 5min removes 3 times of volumes of addition after insoluble impurities is centrifuged under speed;
(2) Essence that step (1) obtains is centrifuged 5min under 5000r/min centrifugal speed and obtains precipitating one, it is molten by precipitating one
It is homogenized 2h after the ultrapure water of 3 times of weight and obtains homogenate two, the suction filtration of homogenate two is obtained into filtrate, is concentrated filtrate to
The 1/5 of original volume obtains concentrate;
(3) centrifugation 3min under concentrate 5000r/min centrifugal speed that step (2) obtains is obtained into supernatant one, to supernatant
Dehydrated alcohol is added in one until the concentration of ethyl alcohol is 85%, it is that snail essence are thick that suction filtration, which obtains precipitating two, after standing overnight
Extract;
(4) the snail essence crude extract for obtaining step (3) is added in the pure water of 3 times of weight, stirs to after being completely dissolved
It is centrifuged 5min removal insoluble impurities under 5000r/min centrifugal speed and obtains supernatant two, supernatant two is used into molecular cut off
Essence is obtained for 3500 bag filter dialysis 48h;
(5) Essence that step (4) obtains is placed on freeze-drying in freeze dryer by being concentrated under reduced pressure into the 1/5 of original volume,
Powdering is milled after taking-up up to snail essence dry powder.
Embodiment 4
Snail essence dry powder, is made of following steps:
(1) by snail mucus be added 2.5 times of weight pure water be homogenized to obtain homogenate one, by one 3500r/min of homogenate from
The ethyl alcohol precipitation Essence that 12min removes 3 times of volumes of addition after insoluble impurities is centrifuged under heart speed;
(2) Essence that step (1) obtains is centrifuged 12min under 3500r/min centrifugal speed and obtains precipitating one, it is molten by precipitating one
It is homogenized 1.5h after the ultrapure water of 2.5 times of weight and obtains homogenate two, the suction filtration of homogenate two is obtained into filtrate, filtrate decompression is dense
Be reduced to original volume 1/4 obtains concentrate;
(3) centrifugation 15min under concentrate 3500r/min centrifugal speed that step (2) obtains is obtained into supernatant one, to supernatant
Dehydrated alcohol is added in one until the concentration of ethyl alcohol is 75%, it is that snail essence are thick that suction filtration, which obtains precipitating two, after standing overnight
Extract;
(4) the snail essence crude extract for obtaining step (3) is added in the pure water of 2.5 times of weight, stirs to after being completely dissolved
It is centrifuged 6min removal insoluble impurities under 3500r/min centrifugal speed and obtains supernatant two, supernatant two is used into molecular cut off
Essence is obtained for 3500 bag filter dialysis 48h;
(5) Essence that step (4) obtains is placed on freeze-drying in freeze dryer by being concentrated under reduced pressure into the 1/4 of original volume,
Powdering is milled after taking-up up to snail essence dry powder.
Experimental example 1: fibrocyte is promoted to increase test
Snail essence dry powder made from embodiment 1 is dissolved into the aqueous solution of various concentration, these aqueous solutions are tested by MTT and are promoted
The case where increasing into fibrocyte, test result is as shown in Figure of description 1, and wherein concentration 1 is 5%, and concentration 2 is 8%, concentration 3
It is 10%.It can be seen that by Figure of description 1, the snail essence dry powder being made by embodiment 1 is dissolved into the aqueous solution of various concentration
Epithelial cell rate of body weight gain shows that snail essence dry powder provided by the invention can effectively facilitate fibrocyte obviously higher than blank group
Growth.
Experimental example 2: antibacterial test
Snail essence dry powder made from embodiment 1 is dissolved into the aqueous solution of various concentration, tests these aqueous solutions to golden yellow
Staphylococcic number of viable, test result is as follows shown in table, and wherein concentration 1 is 5%, and concentration 2 is 8%, and concentration 3 is 10%.Under
Table can be seen that the number of viable that the snail essence dry powder being made by embodiment 1 is dissolved into the aqueous solution of various concentration is obviously few
In blank group, show that snail essence dry powder provided by the invention has preferable sterilization antibacterial ability.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (7)
1. a kind of snail essence dry powder, it is characterised in that: be made of following steps:
(1) it is homogenized the pure water that 1-3 times of weight is added in snail mucus to obtain homogenate one, homogenate one is centrifuged 5-15min
Essence is precipitated in the ethyl alcohol that 3 times of volumes are added after removal insoluble impurities;
(2) the Essence centrifugation 5-15min that step (1) obtains is obtained into precipitating one, is homogenized after precipitating one is dissolved in ultrapure water
0.5-2h obtains homogenate two, the suction filtration of homogenate two is obtained filtrate, the 1/5-1/2 for concentrating filtrate to original volume is obtained
Concentrate;
(3) the concentrate centrifugation 3-20min that step (2) obtains is obtained into supernatant one, dehydrated alcohol is added into supernatant one
Filtering until the concentration of ethyl alcohol is 60-85%, after standing overnight and obtaining precipitating two is snail essence crude extract;
(4) the snail essence crude extract for obtaining step (3) is added in pure water, and stirring is centrifuged 5-10min to after being completely dissolved
Removal insoluble impurities obtains supernatant two, and the bag filter dialysis 48h that supernatant two is 3500 with molecular cut off is obtained essence
Magnificent liquid;
(5) 1/5-1/2 that the Essence process that step (4) obtains is concentrated under reduced pressure into original volume freezing in freeze dryer is placed on to do
Dry, powdering of milling after taking-up is up to snail essence dry powder.
2. a kind of snail essence dry powder according to claim 1, it is characterised in that: in the step (1), homogenate one
Centrifugal speed is 3000-5000r/min.
3. a kind of snail essence dry powder according to claim 1, it is characterised in that: in the step (2), Essence from
Heart speed is 3000-5000r/min, and precipitating one is 1:1-3 with the weight ratio of ultrapure water.
4. a kind of snail essence dry powder according to claim 1, it is characterised in that: in the step (3), concentrate from
Heart speed is 3000-5000r/min.
5. a kind of snail essence dry powder according to claim 1, it is characterised in that: in the step (4), snail essence is thick
Extract and the weight ratio of pure water are 1:1-3, and the speed of centrifugation is 3000-5000r/min.
6. the preparation method of snail essence dry powder described in -5 any one according to claim 1, it is characterised in that: including following
Step:
(1) it will be cleaned up after snail decladding, the pure water that 1-3 times of weight is added is homogenized to obtain homogenate one, by homogenate one
It is centrifuged the ethyl alcohol precipitation Essence that 5-15min removes 3 times of volumes of addition after insoluble impurities;
(2) the Essence centrifugation 5-15min that step (1) obtains is obtained into precipitating one, is homogenized after precipitating one is dissolved in ultrapure water
0.5-2h obtains homogenate two, the suction filtration of homogenate two is obtained filtrate, the 1/5-1/2 for concentrating filtrate to original volume is obtained
Concentrate;
(3) the concentrate centrifugation 3-20min that step (2) obtains is obtained into supernatant one, dehydrated alcohol is added into supernatant one
Filtering until the concentration of ethyl alcohol is 60-85%, after standing overnight and obtaining precipitating two is snail essence crude extract;
(4) the snail essence crude extract for obtaining step (3) is added in pure water, and stirring is centrifuged 5-10min to after being completely dissolved
Removal insoluble impurities obtains supernatant two, and the bag filter dialysis 48h that supernatant two is 3500 with molecular cut off is obtained essence
Magnificent liquid;
(5) 1/5-1/2 that the Essence process that step (4) obtains is concentrated under reduced pressure into original volume freezing in freeze dryer is placed on to do
Dry, powdering of milling after taking-up is up to snail essence dry powder.
7. according to claim 1 snail essence dry powder described in -5 any one moisturizing essence, nti-freckle Essence face cream or
Application in cleawhite over-night essences facial mask.
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Application publication date: 20191108 |