CN110408675A - A kind of experimental method measuring Chemotaxis of Bacteria - Google Patents
A kind of experimental method measuring Chemotaxis of Bacteria Download PDFInfo
- Publication number
- CN110408675A CN110408675A CN201910695873.1A CN201910695873A CN110408675A CN 110408675 A CN110408675 A CN 110408675A CN 201910695873 A CN201910695873 A CN 201910695873A CN 110408675 A CN110408675 A CN 110408675A
- Authority
- CN
- China
- Prior art keywords
- pipette tips
- chemotaxis
- agar
- measurement
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 66
- 238000002474 experimental method Methods 0.000 title claims abstract description 39
- 230000035605 chemotaxis Effects 0.000 title claims abstract description 30
- 229920001817 Agar Polymers 0.000 claims abstract description 42
- 239000008272 agar Substances 0.000 claims abstract description 42
- 239000005667 attractant Substances 0.000 claims abstract description 34
- 230000031902 chemoattractant activity Effects 0.000 claims abstract description 29
- 238000005259 measurement Methods 0.000 claims abstract description 21
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- 239000002975 chemoattractant Substances 0.000 claims description 4
- 230000003399 chemotactic effect Effects 0.000 claims description 4
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000003698 anagen phase Effects 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 229920000609 methyl cellulose Polymers 0.000 claims description 3
- 239000001923 methylcellulose Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 6
- 238000011156 evaluation Methods 0.000 abstract 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 14
- 238000000034 method Methods 0.000 description 11
- 239000011521 glass Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 230000029305 taxis Effects 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Abstract
The invention discloses a kind of experimental methods for measuring Chemotaxis of Bacteria, the agar containing attractant is drawn using the pipette tips of liquid-transfering gun, attractant makes the bacterium for experiencing attractant enter pipette tips after spreading, it reuses liquid-transfering gun to get the agar in pipette tips, passes through ATP evaluation experimental result in measurement agar；It is simple to operate, security risk is not present, and experimental material is easy to get, while evaluating into the cell relative number in pipette tips by the concentration of measurement ATP, compared with the mode for directly counting number under the microscope, operation is more simple and convenient, and experimental result is more acurrate.
Description
Technical field
The present invention relates to bacterium taxis experimental technique fields, more particularly to a kind of experiment side for measuring Chemotaxis of Bacteria
Method.
Background technique
Chemotaxis (also referred to as chemotaxis) is one kind of taxis, refers to soma, bacterium and other are slender
The movement that born of the same parents, multicellular organism tend to according to certain chemical substances in environment.Taxis finds energy source object to bacterium etc.
Matter (such as glucose) and pathogenecity are particularly significant, and bacterium tends to the ground for having higher-energy source material molecular concentration with this
Side, or the place far from toxic (such as phenol).
Currently, mostly being inhaled using glass capillary when measuring chemotactic sexuality of the bacterium to different attractants in the lab
The agar containing attractant is taken, then glass capillary is put into inoculum, and is taken out after a certain time, then aobvious
The cell for entering capillary is counted under micro mirror, that is, can determine that the chemotactic sexuality of bacterium；This method exists asks as follows
Topic: first is that drawing attractant using glass capillary, attractant is not easily taken out in glass capillary, and can only be by capillary when observation
Glass tube is smashed, so that the bacterium into glass capillary releases completely, it is time-consuming and laborious, it wastes equipment and there are a Dingan County
Full blast danger；Second is that using measuring under the microscope into number of bacteria in attractant directly by the way of number numbers, it is time-consuming and laborious, and
Easily there are errors.
Therefore operating how the measurement experiment of Chemotaxis of Bacteria, simpler and result is more acurrate, is those skilled in the art
The technical issues that need to address at present.
Summary of the invention
The object of the present invention is to provide a kind of experimental methods for measuring Chemotaxis of Bacteria, are drawn using the pipette tips of liquid-transfering gun
With get the agar containing attractant, and the concentration by measuring ATP is evaluated into the cell relative number in pipette tips, operation
It is more simple and convenient, and experimental result is more acurrate.
In order to solve the above technical problems, the present invention provides a kind of experimental method for measuring Chemotaxis of Bacteria, comprising:
S1: the bacterium with locomitivity is resuspended in the first centrifuge tube after rinsing；
S2: being drawn in the agar containing preset concentration attractant to its pipette tips using liquid-transfering gun, and the pipette tips are inserted into institute
It states in the first centrifuge tube, and is immersed in the tip of the pipette tips in bacterial suspension；
S3: after standing preset time, the pipette tips are taken out out of described first centrifuge tube；
S4: the agar in the pipette tips is got to the second centrifuge tube using the liquid-transfering gun；
S5: measuring and calculating enters the cell relative number in agar, and then determines bacterium to the chemotaxis of corresponding chemical attractant
Ability.
Preferably, the cell relative number that measuring and calculating enters in agar specifically includes: by ATP in kit measurement agar
Concentration, to calculate the cell relative number into the pipette tips.
Preferably, the bacterium with locomitivity is resuspended in the first centrifuge tube after rinsing and is specifically included:
Twice using PBS buffer solution centrifugal rinsing by the bacterium of 1mL culture to logarithmic growth phase；
Bacterium is resuspended in the first centrifuge tube of 1.5mL using the 100 μ LPSF buffers containing 0.3% methylcellulose
In.
Preferably, before being drawn in the agar containing preset concentration attractant to its pipette tips using liquid-transfering gun further include:
Preset length is clipped at the tip of pipette tips.
Preferably, after the pipette tips are inserted into first centrifuge tube further include:
In the nozzle upper cover upper tube cap of first centrifuge tube.
Preferably, between step S3 and S4 further include: wipe the bacterium on the pipette tips surface with cotton ball soaked in alcohol.
Preferably, the attractant includes Glc, Met, Ser, Pro and PC.
Preferably, the range of the preset time is 25-35min.
Preferably, all experimentss operation carries out in Biohazard Safety Equipment, and the range of experimental temperature is 36-38 DEG C.
The experimental method of measurement Chemotaxis of Bacteria provided by the invention contains attractant using the pipette tips absorption of liquid-transfering gun
Agar, attractant make the bacterium for experiencing attractant enter pipette tips after diffusion, recycle liquid-transfering gun by the agar in pipette tips
It gets, it is simple to operate, security risk is not present, and experimental material is easy to get；It is commented simultaneously by measuring the concentration of ATP
Surely enter the cell relative number in pipette tips, compared with the mode for directly counting number under the microscope, operate more simple and convenient, experiment
As a result more acurrate.
Detailed description of the invention
Fig. 1 is a kind of flow chart of specific embodiment of measurement Chemotaxis of Bacteria experimental method provided by the present invention；
Fig. 2 is that pipette tips are inserted into the structural schematic diagram in the first centrifuge tube in the operating process for measure Chemotaxis of Bacteria experiment；
ATP concentration is shown in agar in the case of the different attractants that Fig. 3 is measured for use experimental method provided by the invention
It is intended to.
It is marked in attached drawing as follows:
First centrifuge tube 1, pipette tips 2, pipe lid 3.
Specific embodiment
Core of the invention is to provide a kind of experimental method for measuring Chemotaxis of Bacteria, is drawn using the pipette tips of liquid-transfering gun
With get the agar containing attractant, and the concentration by measuring ATP is evaluated into the cell relative number in pipette tips, operation
It is more simple and convenient, and experimental result is more acurrate.
In order to enable those skilled in the art to better understand the solution of the present invention, with reference to the accompanying drawings and detailed description
The present invention is described in further detail.
Fig. 1 to Fig. 3 is please referred to, Fig. 1 is a kind of specific reality of measurement Chemotaxis of Bacteria experimental method provided by the present invention
Apply the flow chart of mode；Fig. 2 is that pipette tips are inserted into the structure in the first centrifuge tube in the operating process for measure Chemotaxis of Bacteria experiment
Schematic diagram；ATP concentration signal in agar in the case of the different attractants that Fig. 3 is measured for use experimental method provided by the invention
Figure.
The experimental method for the measurement Chemotaxis of Bacteria that the specific embodiment of the invention provides, mainly includes the following steps:
S1: the bacterium with locomitivity is resuspended in the first centrifuge tube 1 after rinsing；
When specific operation, the inoculum of certain volume can be first taken from Micro-Organism Culture Dish, and utilizes PBS buffer solution
(i.e. phosphate buffered saline solution) centrifugal rinsing twice, then recycles the PSF buffer containing 0.3% methylcellulose by bacterium
It is resuspended in the first centrifuge tube 1.
In experimental method provided by the invention, it is preferable to use the bacterium that logarithmic growth phase is arrived in culture, the groups of logarithmic phase for bacterium
Body cell has many advantages, such as that physiological property ingredient balance more each than more consistent, cell increases and growth rate is constant, is metabolism, physiology
The good material of research, and the optimal material as strain.
In addition, the application is not specifically limited the type of bacterium, it can select spiral shell substance etc. is any there is movement energy
The bacterium of power.
S2: being drawn in the agar containing preset concentration attractant to its pipette tips 2 using liquid-transfering gun, and pipette tips 2 are inserted into first
In centrifuge tube 1, and it is immersed in the tip of the pipette tips in bacterial suspension.
It should be noted that the top of pipette tips 2 is higher than bacterial suspension liquid when pipette tips 2 are inserted into the first centrifuge tube 1
The tip in face, only pipette tips 2 is immersed in bacterial suspension.
In addition, the application is not specifically limited the type of attractant, used in different bacterium there are many types of attractant
Attractant is not exactly the same, specifically can select attractant according to bacterial species；The concentration of attractant specifically may be used in agar
To need sets itself according to experiment, the application is not specifically limited this.
S3: after standing preset time, pipette tips 2 are taken out out of first centrifuge tube 1；
S4: the agar in pipette tips 2 is got to the second centrifuge tube using liquid-transfering gun.
When specific operation, after drawing in agar to its pipette tips 2 using liquid-transfering gun, directly liquid-transfering gun can be utilized pipette tips
2 squeeze into the first centrifuge tube 1, and pipette tips 2 is made to be immersed in the bacterial suspension in the first centrifuge tube 1 filled with the tip of attractant
In；After standing preset time and taking out pipette tips 2 out of first centrifuge tube 1, first pipette tips 2 are mounted on liquid-transfering gun, cotton ball soaked in alcohol
After the bacterium for wiping 2 surface of pipette tips, liquid-transfering gun is recycled to get the agar in pipette tips 2.
During standing, the chemical substance in 2 tip of pipette tips is spread around, and the bacterium for experiencing chemical substance then can court
To 2 tip motion of pipette tips and enter in the agar inside pipette tips 2.
Pollution of the agar by external environment, operating process carry out in Biohazard Safety Equipment when wherein, to avoid standing, In
It, can be in the nozzle upper cover upper tube cap 3 of the first centrifuge tube 1, when reaching standing after the first centrifuge tube 1 is inserted at the tip of pipette tips 2
Between after, then open pipe lid 3 take out pipette tips 2.
In addition, can be with the taxis of Accurate Determining bacterium for guarantee, time of repose is unsuitable too short also unsuitable too long, preferably
Ground, time of repose can be located at 30min or so.
S5: measuring and calculating enters the cell relative number in agar, and then determines bacterium to the chemotaxis of corresponding chemical attractant
Ability.
Wherein it should be noted that bacterium is unicellular microorganism, therefore the cell relative number in entrance pipette tips 2, that is, thin
Bacterium relative number.
It preferably, can be opposite into the cell in pipette tips 2 to calculate by the concentration of ATP in kit measurement agar
Number, and then determine bacterium to the ability of the chemotaxis of corresponding chemical attractant.
Wherein, ATP refers to adenosine triphyosphate (abbreviation atriphos), is most direct energy in organism
Source according to ATP content and then can be calculated since ATP content is roughly the same and relatively stable in each bacterial cell
Bacterial number.And ATP concentration is generally measured using fluorescein-luciferase biloluminescence method at present.
The experimental method of measurement Chemotaxis of Bacteria provided by the invention contains attractant using the absorption of pipette tips 2 of liquid-transfering gun
Agar, after making bacterium enter pipette tips 2 using attractant, recycle liquid-transfering gun the agar in pipette tips 2 is got, it is easy to operate
It is convenient, security risk is not present, and liquid-transfering gun is reusable；And it is evaluated by the concentration of measurement ATP into pipette tips 2
Cell relative number, compared with the mode for directly counting number under the microscope, operation is more simple and convenient, and experimental result is more acurrate.
It should be noted that for guarantee experimental result accuracy, whole experiment process need in Biohazard Safety Equipment into
Row, and experimental temperature is arranged at 37 DEG C or so, to guarantee the activity of bacterium.
On the basis of above-mentioned specific embodiment, in experimental method provided by the invention, drawn using liquid-transfering gun
Before agar, certain length first can be clipped at the tip of the pipette tips of liquid-transfering gun 2, to increase the size of the opening of pipette tips 2, convenient for drawing
Agar and get agar.
In experimental method provided by the invention, it should be noted that the application is to inoculum used in experiment, fine jade
The specification of volume, liquid-transfering gun, the first centrifuge tube 1 of rouge etc. is not specifically limited, and can specifically adjust choosing according to the actual situation
With.In a specific embodiment, 1mL inoculum can be taken from bacteria culture bottle, utilize buffer will after rinsing
Bacterium is resuspended in the first centrifuge tube 1 of 1.5mL, and the volume of bacterial suspension is about 100 μ L after resuspension, while selecting 200mL
Liquid-transfering gun, after 13mm or so is clipped at the tip of pipette tips 2 used in it, draw the about 10mL of the agar containing attractant with liquid-transfering gun
To pipette tips 2,2 tip of pipette tips that then will be filled with agar again is inserted into the spiral shell substance suspension in the first centrifuge tube 1,37
DEG C incubate 30 minutes after, recycle 2 tip of pipette tips agar simultaneously measure the ATP concentration in agar.
When measuring chemotactic sexuality of certain bacterium to different attractants using experimental method provided by the invention, only need
The agar containing different attractants is successively selected to be tested, other experiment conditions are identical；It is measured when selecting spiral shell substance to be used as
Target, and successively select Glc (glucose), Met (methionine), Ser (serine), Pro (proline) and PC (lecithin)
As attractant, PSF buffer (formula of 100mL PSF buffer be 100mLPBS buffer be added 0.5% fructose and
5% sorbierite) when being used as control material, ATP concentration is as shown in Figure 3 in measured agar.
In the description of the present patent application file, in the present specification, such as first and second etc relational terms are only
Only it is used to distinguish an entity and other several entities, appoints without necessarily requiring or implying existing between these entities
What this actual relationship or sequence.
In addition, each embodiment is described in a progressive manner in specification, the highlights of each of the examples are with
The difference of other embodiments, similar portion may refer to each other between each embodiment.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is relatively simple, related place is referring to method part illustration
.
The experimental method of measurement Chemotaxis of Bacteria provided by the present invention is described in detail above.It is used herein
A specific example illustrates the principle and implementation of the invention, and the above embodiments are only used to help understand
Method and its core concept of the invention.It should be pointed out that for those skilled in the art, not departing from this
, can be with several improvements and modifications are made to the present invention under the premise of inventive principle, these improvement and modification also fall into the present invention
In scope of protection of the claims.
Claims (9)
1. a kind of experimental method for measuring Chemotaxis of Bacteria characterized by comprising
S1: the bacterium with locomitivity is resuspended in the first centrifuge tube after rinsing；
S2: being drawn in the agar containing preset concentration attractant to its pipette tips using liquid-transfering gun, by pipette tips insertion described the
In one centrifuge tube, and it is immersed in the tip of the pipette tips in bacterial suspension；
S3: after standing preset time, the pipette tips are taken out out of described first centrifuge tube；
S4: the agar in the pipette tips is got to the second centrifuge tube using the liquid-transfering gun；
S5: measuring and calculating enters the cell relative number in agar, and then determines bacterium to the chemotactic sexuality of corresponding chemical attractant.
2. the experimental method of measurement Chemotaxis of Bacteria according to claim 1, which is characterized in that measuring and calculating enters in agar
Cell relative number specifically includes: by the concentration of ATP in kit measurement agar, to calculate the cell into the pipette tips
Relative number.
3. the experimental method of measurement Chemotaxis of Bacteria according to claim 2, which is characterized in that will be with locomitivity
Bacterium is resuspended in the first centrifuge tube after rinsing and specifically includes:
Twice using PBS buffer solution centrifugal rinsing by the bacterium of 1mL culture to logarithmic growth phase；
Bacterium is resuspended in the first centrifuge tube of 1.5mL using the 100 μ LPSF buffers containing 0.3% methylcellulose.
4. the experimental method of measurement Chemotaxis of Bacteria according to claim 2, which is characterized in that drawn using liquid-transfering gun
Before in agar containing preset concentration attractant to its pipette tips further include:
Preset length is clipped at the tip of pipette tips.
5. the experimental method of measurement Chemotaxis of Bacteria according to claim 4, which is characterized in that be inserted by the pipette tips
After in first centrifuge tube further include:
In the nozzle upper cover upper tube cap of first centrifuge tube.
6. the experimental method of measurement Chemotaxis of Bacteria according to claim 5, which is characterized in that between step S3 and S4
Further include: the bacterium on the pipette tips surface is wiped with cotton ball soaked in alcohol.
7. the experimental method of measurement Chemotaxis of Bacteria according to claim 1, which is characterized in that the attractant includes
Glc, Met, Ser, Pro and PC.
8. the experimental method of measurement Chemotaxis of Bacteria according to claim 1, which is characterized in that the model of the preset time
It encloses for 25-35min.
9. the experimental method of measurement Chemotaxis of Bacteria according to claim 8, which is characterized in that all experimentss are operated in life
It is carried out in object safety cabinet, the range of experimental temperature is 36-38 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910695873.1A CN110408675A (en) | 2019-07-30 | 2019-07-30 | A kind of experimental method measuring Chemotaxis of Bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910695873.1A CN110408675A (en) | 2019-07-30 | 2019-07-30 | A kind of experimental method measuring Chemotaxis of Bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110408675A true CN110408675A (en) | 2019-11-05 |
Family
ID=68364327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910695873.1A Pending CN110408675A (en) | 2019-07-30 | 2019-07-30 | A kind of experimental method measuring Chemotaxis of Bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110408675A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263223A (en) * | 2005-09-16 | 2008-09-10 | 通用电气医疗集团生物科学公司 | Cell migration assay |
| WO2012019436A1 (en) * | 2010-08-10 | 2012-02-16 | Capitalbio Corporation | Microfluidic device for cell motility screening and chemotaxis testing |
| CN104792750A (en) * | 2015-03-27 | 2015-07-22 | 南方医科大学 | Construction method of cell polarity model |
-
2019
- 2019-07-30 CN CN201910695873.1A patent/CN110408675A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263223A (en) * | 2005-09-16 | 2008-09-10 | 通用电气医疗集团生物科学公司 | Cell migration assay |
| WO2012019436A1 (en) * | 2010-08-10 | 2012-02-16 | Capitalbio Corporation | Microfluidic device for cell motility screening and chemotaxis testing |
| CN104792750A (en) * | 2015-03-27 | 2015-07-22 | 南方医科大学 | Construction method of cell polarity model |
Non-Patent Citations (4)
| Title |
|---|
| IGEM 2010: "Bacterial Crowding：Capillary assays", 《IGEM 2010》 * |
| LASZLO KOHIDAI等: "Molecule Dependent Chemotactic Responses of Tetrahymena pyriformis Elicited by Volatile Oils", 《ACTA PROTOZOOLOGICA》 * |
| 李涛: "假单胞菌趋化性研究概述", 《天津农业科学》 * |
| 程纯明，李欣瑶: "第十届世界华人虾蟹研讨会专题：上海海大群英荟萃，虾蟹产业的病害危机是否触碰到你的神经", 《当代水产》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Avesar et al. | Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays | |
| JP4642893B2 (en) | Method, device and system for counting white blood cells per volume | |
| CN104459148B (en) | Analysis | |
| CN102460177B (en) | For the system and method automatically ventilating to cultivation sample receiver and sample | |
| CN201476980U (en) | Device for sampling and testing | |
| CN104677963B (en) | A kind of Cu for being applied to non-damage micrometering system2+Selectivity micro-electrode and preparation method thereof | |
| CN110234749A (en) | Analyze and use the motility kinematics of microorganism | |
| CN103945941A (en) | System and apparatus for reactions | |
| CN109313156A (en) | Test macro for analysing body fluid sample | |
| CN104135927A (en) | Blood-sampling tube with integrated sensor device | |
| JPH09101304A (en) | Test tube ffor measuring sedimentation velocity of erythrocyte | |
| Andredaki et al. | Numerical investigation of quasi-sessile droplet absorption into wound dressing capillaries | |
| US4248830A (en) | Device for microbiological testing | |
| CN110408675A (en) | A kind of experimental method measuring Chemotaxis of Bacteria | |
| CN208313886U (en) | The flow path system of biosensor analysis instrument | |
| JP2002062250A (en) | Device and method for supplying sheath fluid, and method of evaluating supply condition of sheath fluid | |
| CN209327056U (en) | A kind of corollary apparatus being loaded for the quantitative sampling of micro-fluidic chip and dilution | |
| Magrisso et al. | Fiber-optic biosensor to assess circulating phagocyte activity by chemiluminescence | |
| CN207976319U (en) | Micro-sampler for medical inspection | |
| CN106047849A (en) | Immobilized microbial system as well as preparation method thereof and water toxicity detection method | |
| CN107255664B (en) | A kind of photic electrochemical sensor preparation method and application of SnSe/SWNT modified electrode detecting chloramphenicol | |
| CN114235769B (en) | Blood cell staining method | |
| CN105671123B (en) | The method of counting of bacterium in a kind of liquid | |
| CN109490283A (en) | Application of four (p-hydroxybenzene) porphyrin-β-CD supermolecules in detection fluorine ion | |
| CN206853707U (en) | A kind of Urine proteins measure uses preservative quantitative adding device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191105 |
|
| RJ01 | Rejection of invention patent application after publication |