CN110408658A - A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix - Google Patents
A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix Download PDFInfo
- Publication number
- CN110408658A CN110408658A CN201910693810.2A CN201910693810A CN110408658A CN 110408658 A CN110408658 A CN 110408658A CN 201910693810 A CN201910693810 A CN 201910693810A CN 110408658 A CN110408658 A CN 110408658A
- Authority
- CN
- China
- Prior art keywords
- white wine
- polyhydroxyalkanoates
- wine wastewater
- wastewater
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 title claims abstract description 49
- 229920000903 polyhydroxyalkanoate Polymers 0.000 title claims abstract description 49
- 239000002351 wastewater Substances 0.000 title claims abstract description 47
- 235000020097 white wine Nutrition 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000011159 matrix material Substances 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 239000010802 sludge Substances 0.000 claims abstract description 11
- 239000012895 dilution Substances 0.000 claims abstract description 10
- 238000010790 dilution Methods 0.000 claims abstract description 10
- 238000005273 aeration Methods 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 230000002194 synthesizing effect Effects 0.000 claims abstract 2
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 abstract description 8
- 239000004033 plastic Substances 0.000 abstract description 8
- 238000004064 recycling Methods 0.000 abstract description 3
- 238000013459 approach Methods 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 239000010815 organic waste Substances 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 229920000388 Polyphosphate Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000001205 polyphosphate Substances 0.000 description 1
- 235000011176 polyphosphates Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000012815 thermoplastic material Substances 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1263—Sequencing batch reactors [SBR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
- C02F2103/325—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters from processes relating to the production of wine products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of using white wine wastewater as the method for matrix production polyhydroxyalkanoates, this method are as follows: toward the white wine wastewater being inoculated with after dilution is added in the batch-type batch reactor of mixed bacteria sludge, react synthesizing polyhydroxyalkanoateby under the conditions of continuous aeration.The method of the present invention produces polyhydroxyalkanoates using the white wine wastewater after dilution as matrix, not only realize the recycling of white wine wastewater, and biological plastics polyhydroxyalkanoates can be quickly produced, a kind of new approaches are provided for the comprehensive development and utilization of city organic waste.
Description
Technical field
The present invention relates to a kind of using white wine wastewater as the method for matrix production polyhydroxyalkanoates, belongs to environmental protection
With application technology as the second resource field.
Background technique
Specialty industries one of of the liquor industry as China produce a large amount of while bringing tremendous economic interests
Pollutant, white wine wastewater.Main By product of the white wine wastewater as liquor production process, yield are very big.According to statistics, every production
1t65%vol white wine will generate about 48t waste water, if do not dealt carefully with to these waste water, will generate to environment huge
Harm, therefore arduous task is disposed in the processing of white wine wastewater.The processing of white wine wastewater at present, which essentially consists in, reduces pollution therein
Object concentration realizes qualified discharge.And containing large amount of organic (sugar and protein etc.) in white wine wastewater, biochemical is higher,
As it is a kind of can the function of resource substance of effective recycling be ignored.Therefore, how can be wide from white wine wastewater high efficiente callback
The general resource substance applied to actual production it is handled disposition it is significant, can not only lower white wine wastewater to the danger of environment
Evil, while realizing the resource utilization of waste.
Polyhydroxyalkanoates (PHA) is a kind of Biopolvester intracellular, can be used as the carbon source and energy substance storage of cell
In intracellular.Compared with the plastics of traditional petrochemical industry synthesis, PHA not only has physicochemical properties similar with its, also has
There are the performances such as totally biodegradable, biocompatibility and thermoplastic materials plasticity, is the ideal material for substituting synthetic plastic, causes
Bio-science field and materialogy field are more and more paid close attention to.It is at present industry using bacterial fermentation process and gene engineering research
The main method of PHA is synthesized, but these method operating costs are high, complex process, seriously restrict the marketization of PHA production.Closely
Utilizing over year becomes research hotspot rich in the activated sludge of a variety of mixed bacterial microorganisms and abundant carbon source production PHA, and
Achieve very big achievement.
Summary of the invention
Goal of the invention: technical problem to be solved by the invention is to provide a kind of produced using white wine wastewater as matrix to gather
The method of hydroxyalkanoate, this method can promote mixed bacterial rapid synthesis to go out a large amount of PHA, and combined coefficient is high.
In order to solve the above technical problems, the technical scheme adopted by the invention is as follows:
A method of polyhydroxyalkanoates, this method are produced using white wine wastewater as matrix are as follows: past to be inoculated with mixing
The white wine wastewater after dilution is added in the batch-type batch reactor of strain sludge, reaction synthesizes poly- hydroxyl under the conditions of continuous aeration
Base alkanoic acid ester.
Wherein, the COD content in the white wine wastewater after dilution is 600~1800mgCOD/L, and preferably COD content is
1200mgCOD/L。
Wherein, the organic loading rate of batch-type batch reactor is 1.2~3.6gCOD/L/d, and preferably organic loading rate is
2.4gCOD/L/d。
Wherein, the single reaction time of batch-type batch reactor is 6 hours.
In batch-type batch reactor operational process, when organic loading rate is 2.4gCOD/L/d, single reaction time is
6 hours, largely PHA can be promoted to produce, improve the generating rate of PHA.
The principle of the method for the present invention are as follows: mixed bacterial can the nutriments such as carbon source relative surplus, nitrogen phosphorus it is opposite lack,
Electron donor and electron acceptor synthesize and store PHA under the conditions of being alternately present etc., to maintain bacterium under nutrient environment non-equilibrium condition
The metastable survival condition of body.When the normal metabolic way of microorganism is suppressed, the cellular material of reserve can be sought in external source
It supports and is utilized when lacking by microorganism, such as polyphosphate, PHA, glucide.White wine wastewater is containing a large amount of organic substrates
Meanwhile there is less the content of nitrogen and phosphorous, therefore micro-organisms PHA can be stimulated as stored substance；Meanwhile white wine wastewater
In the microelements such as the calcium, magnesium, the potassium that contain, many key enzymes and functional microorganism during synthesis PHA can be effectively improved
Activity, in this way while handling white wine wastewater, and secondary use is realized to organic matter contained therein, reduce
The production cost of PHA, is conducive to the popularization and application of PHA.
Compared with the prior art, it is had the beneficial effect that possessed by technical solution of the present invention
Firstly, the method for the present invention produces polyhydroxyalkanoates using the white wine wastewater after dilution as matrix, not only realize
The recycling of white wine wastewater, and can quickly produce biological plastics polyhydroxyalkanoates, it is the comprehensive of city organic waste
It closes development and utilization and a kind of new approaches is provided；
Secondly, can effectively be absorbed using batch-type batch reactor and mixed bacteria sludge organic in white wine wastewater
Object can also produce PHA while realizing discharged wastewater met the national standard, therefore the method for the present invention is reducing PHA production cost
Effective resource utilization can also be carried out to white wine wastewater simultaneously；
Finally, biological plastics polyhydroxyalkanoates is stored substance important in extracellular microbial, there is very high exploitation value
Value, it is the important source material of synthesising biological plastics, can effectively substitute petroleum plastics, and the method for the present invention finally realizes pollutant
The effect turned waste into wealth.
Specific embodiment
The present invention is described in further details combined with specific embodiments below.
The activated sludge used in the embodiment of the present invention 1~3 derives from Nanjing sewage plant secondary settling tank sludge, and white wine is useless
Water derives from the waste water generated in the white wine process of factory production of Nanjing.
Embodiment 1
The method that the present invention produces polyhydroxyalkanoates as matrix using white wine wastewater, specifically:
In the organic glass batch-type batch reactor that swept volume is 3L, mixed bacteria sludge and 1.5L are sequentially added
White wine wastewater after dilution, the COD content in white wine wastewater is 600mgCOD/L, reaches organic loading rate in reactor
1.2gCOD/L/d；
The maximum value of PHA yield was obtained at dissolved oxygen zooming 27 minutes, the maximum level of PHA is 3.756%,
PHA yield is 0.115g/g.In reactor, aeration quantity 100L/h；Control water inlet pH value is 7, and control reaction temperature is 25 ± 1
℃。
Embodiment 2
The method that the present invention produces polyhydroxyalkanoates as matrix using white wine wastewater, specifically:
In the organic glass batch-type batch reactor that swept volume is 3L, mixed bacteria sludge (activity is sequentially added
Sludge) and 1.5L dilution after white wine wastewater, the COD content in white wine wastewater is 1200mgCOD/L, is made organic negative in reactor
Lotus rate reaches 2.4gCOD/L/d；
The maximum value of PHA yield was obtained at dissolved oxygen zooming 23 minutes, the maximum level of PHA is 2.591%,
PHA yield is 0.226g/g.In reactor, aeration quantity 100L/h；Control water inlet pH value is 7, and control reaction temperature is 25 ± 1
℃。
Embodiment 3
The method that the present invention produces polyhydroxyalkanoates as matrix using white wine wastewater, specifically:
In the organic glass batch-type batch reactor that swept volume is 3L, mixed bacteria sludge and 1.5L are sequentially added
White wine wastewater after dilution, the COD content in white wine wastewater is 1800mgCOD/L, reaches organic loading rate in reactor
3.6gCOD/L/d；
The maximum value of PHA yield was obtained at dissolved oxygen zooming 40 minutes, the maximum level of PHA is 3.985%,
PHA yield is 0.152g/g.In reactor, aeration quantity 100L/h；Control water inlet pH value is 7, and control reaction temperature is 25 ± 1
℃。
1 PHA yield experimental summary of table
In table 1, MLVSS: volatile suspended matter solid concentration；WV: dischargeable capacity；OLR: organic loading rate.
By table 1 it is found that producing polyhydroxyalkanoates using the white wine wastewater after dilution as matrix, can not only realize white
The resource utilization of wine waste water, moreover it is possible to effectively produce biological plastics polyhydroxyalkanoates.In batch-type batch reactor, control
Aeration quantity processed is 100L/h；Control water inlet pH value is 7, and control reaction temperature is 25 ± 1 DEG C, when OLR is 2.4gCOD/L/d,
Maximum PHA content is that 2.591%, PHA yield is 0.226g/g.
Claims (5)
1. a kind of using white wine wastewater as the method for matrix production polyhydroxyalkanoates, it is characterised in that: this method are as follows: past to connect
Kind has the white wine wastewater being added after dilution in the batch-type batch reactor of mixed bacteria sludge, reacts under the conditions of continuous aeration
Synthesizing polyhydroxyalkanoateby.
2. according to claim 1 using white wine wastewater as the method for matrix production polyhydroxyalkanoates, feature exists
In: the COD content in diluted white wine wastewater is 600~1800mgCOD/L.
3. according to claim 1 using white wine wastewater as the method for matrix production polyhydroxyalkanoates, feature exists
In: the organic loading rate of batch-type batch reactor is 1.2~3.6gCOD/L/d.
4. according to claim 1 using white wine wastewater as the method for matrix production polyhydroxyalkanoates, feature exists
In: the single reaction time of batch-type batch reactor is 6 hours.
5. according to claim 1 using white wine wastewater as the method for matrix production polyhydroxyalkanoates, feature exists
In: in batch-type batch reactor, aeration quantity 100L/h；PH value of intaking is 7, and reaction temperature is 25 ± 1 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910693810.2A CN110408658A (en) | 2019-07-30 | 2019-07-30 | A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910693810.2A CN110408658A (en) | 2019-07-30 | 2019-07-30 | A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110408658A true CN110408658A (en) | 2019-11-05 |
Family
ID=68364160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910693810.2A Pending CN110408658A (en) | 2019-07-30 | 2019-07-30 | A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110408658A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111807637A (en) * | 2020-07-30 | 2020-10-23 | 北京工业大学 | Reaction device and method for producing PHA (polyhydroxyalkanoate) by recycling organic matter mainstream of beer wastewater |
| CN112359072A (en) * | 2020-10-29 | 2021-02-12 | 国河环境研究院(南京)有限公司 | Method for producing PHA (polyhydroxyalkanoate) by utilizing excess sludge and liquor wastewater fermentation product and taking nitrous oxide as electron acceptor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178529A (en) * | 2014-07-09 | 2014-12-03 | 北京工业大学 | Method for producing PHA by continuous treatment of molasses alcohol wastewater via mixed halophilic bacteria |
| CN106186619A (en) * | 2016-08-17 | 2016-12-07 | 三明学院 | A kind of sludge organism synthesis of hydroxy butanoic acid and the method for Hydroxycaprylic acid copolymer |
| CN108103114A (en) * | 2017-12-28 | 2018-06-01 | 北京工业大学 | A kind of method for inhibiting halophilic archaea EPS output increased PHA yield |
-
2019
- 2019-07-30 CN CN201910693810.2A patent/CN110408658A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178529A (en) * | 2014-07-09 | 2014-12-03 | 北京工业大学 | Method for producing PHA by continuous treatment of molasses alcohol wastewater via mixed halophilic bacteria |
| CN106186619A (en) * | 2016-08-17 | 2016-12-07 | 三明学院 | A kind of sludge organism synthesis of hydroxy butanoic acid and the method for Hydroxycaprylic acid copolymer |
| CN108103114A (en) * | 2017-12-28 | 2018-06-01 | 北京工业大学 | A kind of method for inhibiting halophilic archaea EPS output increased PHA yield |
Non-Patent Citations (1)
| Title |
|---|
| 傅海霞等: "利用聚糖菌活性污泥***处理酿酒废水的试验研究", 《工业水处理》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111807637A (en) * | 2020-07-30 | 2020-10-23 | 北京工业大学 | Reaction device and method for producing PHA (polyhydroxyalkanoate) by recycling organic matter mainstream of beer wastewater |
| CN111807637B (en) * | 2020-07-30 | 2022-05-03 | 北京工业大学 | Reaction device and method for producing PHA (polyhydroxyalkanoate) by recycling organic matter mainstream of beer wastewater |
| CN112359072A (en) * | 2020-10-29 | 2021-02-12 | 国河环境研究院(南京)有限公司 | Method for producing PHA (polyhydroxyalkanoate) by utilizing excess sludge and liquor wastewater fermentation product and taking nitrous oxide as electron acceptor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Keskin et al. | Photofermentative hydrogen production from wastes | |
| Khanna et al. | Biohydrogen production by dark fermentation | |
| CN106477846B (en) | Method for strengthening microbial electrolysis sludge hydrogen production and recycling struvite by adopting free nitrite | |
| CN100357174C (en) | Heat treatment-fermented hy drogen-generating method by preparing hydrogen from residual sludge for sewage treatment plant | |
| CN101250554B (en) | Method for improving hydrogen gas yield of kitchen waste anaerobic digestion | |
| Mohammadi et al. | High-rate fermentative hydrogen production from palm oil mill effluent in an up-flow anaerobic sludge blanket-fixed film reactor | |
| Kumar et al. | Continuous biogenic hydrogen production from dilute acid pretreated algal hydrolysate using hybrid immobilized mixed consortia | |
| CN110564783B (en) | Method for producing polyhydroxyalkanoate by combining excess sludge and white spirit wastewater | |
| CN110408658A (en) | A method of polyhydroxyalkanoates is produced using white wine wastewater as matrix | |
| Wannapokin et al. | Potential of bio-hydrogen production by C. pasteurianum co-immobilized with selected nano-metal particles | |
| Kumar et al. | Improved hydrogen production from galactose via immobilized mixed consortia | |
| KR101990059B1 (en) | Apparatus and method of producing high purity methane gas using gas recycle | |
| Markov et al. | Spiral tubular bioreactors for hydrogen production by photosynthetic microorganisms: design and operation | |
| CN106978346A (en) | A kind of method of lifting PHA synthesis bacteriums output section strain discharge capacity | |
| CN116732111A (en) | Method for promoting conversion of waste biomass energy by adopting persulfate oxidation and sulfate reduction bacteria novel system | |
| Hamer | Lysis and “cryptic” growth in wastewater and sludge treatment processes | |
| CN101402926B (en) | Biological reinforcing method of hydrogenogen compensating material cultivation and biological hydrogen production system | |
| CN1321174C (en) | Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method | |
| CN112661374B (en) | Method for improving methane yield in sludge anaerobic digestion process by using micro-plastic | |
| CN113104991B (en) | Two-stage co-anaerobic treatment process for simultaneously treating N, N-dimethylformamide and sludge | |
| Sekoai et al. | Elucidating the role of biofilm-forming microbial communities in fermentative biohydrogen process: An overview. Microorganisms. 2022 | |
| CN109355319A (en) | A method of short chain fatty acids are efficiently produced using white wine wastewater | |
| Häggström | Fermentations by immobilized strict anaerobes | |
| Gokhale et al. | Biohydrogen Production Using Cheese Industry Waste: Current Trends and Challenges | |
| CN113604401B (en) | Method for enriching and expanding culture of mutually-cultured acetic acid oxidizing bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191105 |