CN110372105A - A kind of complex micro organism fungicide and preparation method thereof for improveing aquaculture system - Google Patents
A kind of complex micro organism fungicide and preparation method thereof for improveing aquaculture system Download PDFInfo
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- CN110372105A CN110372105A CN201910624847.XA CN201910624847A CN110372105A CN 110372105 A CN110372105 A CN 110372105A CN 201910624847 A CN201910624847 A CN 201910624847A CN 110372105 A CN110372105 A CN 110372105A
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- 244000005700 microbiome Species 0.000 title claims abstract description 40
- 238000009360 aquaculture Methods 0.000 title claims abstract description 38
- 230000000855 fungicidal effect Effects 0.000 title claims abstract description 36
- 239000000417 fungicide Substances 0.000 title claims abstract description 36
- 244000144974 aquaculture Species 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 230000001580 bacterial effect Effects 0.000 claims abstract description 29
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 12
- 241000193752 Bacillus circulans Species 0.000 claims abstract description 10
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 10
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 9
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 9
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 8
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 6
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 49
- 230000004151 fermentation Effects 0.000 claims description 49
- 239000007788 liquid Substances 0.000 claims description 39
- 239000012530 fluid Substances 0.000 claims description 29
- 238000004108 freeze drying Methods 0.000 claims description 17
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 230000000813 microbial effect Effects 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Chinese gallotannin Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims description 10
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 2
- 239000003223 protective agent Substances 0.000 claims description 2
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 244000144972 livestock Species 0.000 abstract description 11
- 239000000126 substance Substances 0.000 abstract description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 4
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 3
- 239000011574 phosphorus Substances 0.000 abstract description 3
- 230000000295 complement effect Effects 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract description 2
- 239000013586 microbial product Substances 0.000 abstract description 2
- 239000005416 organic matter Substances 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000238557 Decapoda Species 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 230000005784 autoimmunity Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- 208000012639 Balance disease Diseases 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 231100000880 dysequilibrium Toxicity 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 125000000018 nitroso group Chemical group N(=O)* 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Farming Of Fish And Shellfish (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to microbial product field, in particular to a kind of complex micro organism fungicide and preparation method thereof for improveing aquaculture system.Microbial inoculum is bacillus subtilis, Rhodopseudomonas palustris, bacillus megaterium, lactobacillus acidophilus, streptococcus fecalis, Bacillus circulans, candida utili, nitrobacteria and denitrifying bacteria hybrid bacterial strain tunning.Include in complex micro organism fungicide in the present invention it is quick the microorganism of dissolved oxygen is provided, and containing the microorganism that can decompose ammonia nitrogen in water body, hydrogen sulfide and nitrite, while also containing the bacterial strain of growth and development needed nutrient matter can be provided for aquatic livestock.Have complementary advantages between each bacterial strain, collaboration plays a role；It quickly can breed to form dominant population after being further applied to water body, macromolecular organic matter, residual bait excrement in fast decoupled aquaculture system etc., the substances such as ammonia nitrogen, phosphorus, nitrite, hydrogen sulfide in water body of degrading, purify water, water body dissolved oxygen content is improved, and pathogenic bacteria can be removed.
Description
Technical field
The invention belongs to microbial product field, in particular to a kind of complex micro organism fungicide for improveing aquaculture system
And preparation method thereof.
Background technique
China is world aquaculture big country, in recent years, as China's culture fishery is quickly grown, cultivates scale not
Disconnected to expand, water pollution is also increasingly severe.Water body is the environment that the existence growth of the aquatic livestocks such as fishes and shrimps must rely on, water quality
Quality be the key that aquaculture especially intensive culture success or failure.The aquaculture majority in China is using high density collection
The aquaculture model of reduction, the activity of the mankind and the secretion of cultivation object itself and excreta, bait surplus, medicament residue etc. because
Element easily causes breeding water body to pollute, water quality factor dysequilibrium, causes aquiculture animal morbidity even high-volume dead.
Improver of water quality used at present mainly includes physical improver of water quality, chemical type improver of water quality and bion
Improver of water quality.Physical improver of water quality mainly passes through suction-operated and quickly adsorbs harmful substance, function and effect
It is slowly unobvious and not degradable.Chemical type improver of water quality can react to make its degradation with harmful substance, but easily
Secondary pollution is formed, and there is irritation and adverse effect to aquatic livestock especially seedling stage animal.Bion improver of water quality
The growth and breeding of pathogenic bacteria, thorough decomposing harmful substances are able to suppress, and microelement can be provided for aquatic livestock, promote aquatic products
Animal autoimmunity repairs the ecological environment of water body.
Bion improver of water quality refers specifically to complex micro organism fungicide, is that by two or more and mutually antagonism does not have
Beneficial microorganism strain is made, and is widely used to the various fields such as aquaculture, sewage treatment.But existing microorganism formulation makes
It being affected with by external conditions such as chemicals residues in environment temperature, water body, throwing is more demanding when applying, and microbial inoculum
Actual effect has larger fluctuation.Microbial strains in the microbial inoculum oxygen consumption when raised growth is bred, will lead in water body and dissolves
The decline of oxygen, the metabolite in growth course can change water body environment, cause a degree of influence to aquatic livestock.
Summary of the invention
The first object of the present invention is to provide a kind of complex micro organism fungicide for improveing aquaculture system.
The second object of the present invention is to provide the preparation method of the complex micro organism fungicide.
To achieve the above object, it is as follows to provide technical solution by the present invention:
A kind of complex micro organism fungicide for improveing aquaculture system, microbial inoculum are bacillus subtilis, the red false unit cell in marsh
Bacterium, bacillus megaterium, lactobacillus acidophilus, streptococcus fecalis, Bacillus circulans, candida utili, nitrobacteria and anti-nitre
Change bacterium hybrid bacterial strain tunning.
The microbial inoculum is that ostreae testa pulverata, biological peptide and freeze drying protectant are added in hybrid bacterial strain fermentation liquid, is centrifuged and receives after mixing
Collection precipitating, obtains the complex micro organism fungicide；Wherein, the quality of ostreae testa pulverata, biological peptide, freeze drying protectant and the fermentation liquid
Than for (1~10): (0.1~5): (10~20): 100.
The biology peptide is corn peptide；Freeze drying protectant is molten for the glycerite and trehalose for being in mass ratio 1-3:1-2
Liquid, wherein glycerite concentration is that 30% aqueous trehalose concentration is 10%.
The fermentation liquid is the illumination at 30-37 DEG C by the inoculum concentration of 3%-5% in fermentation liquid base fluid by hybrid bacterial strain
Culture 5-8 days, until total strain density in fermentation liquid reaches (1~10) × 108Stop fermentation when a/L；Wherein, fermentation liquid base fluid
According to parts by weight, acetic acid 30-50 parts, 50-70 parts of ethyl alcohol, 150-200 parts of sucrose water, 10-30 parts of magnesium sulfate, potassium dihydrogen phosphate
20-50 parts, 50-100 parts of sodium chloride, 20-50 parts of sodium carbonate, 150-300 parts of glucose and 300-500 parts of distilled water.
The hybrid bacterial strain is according to parts by weight, 8-12 parts of bacillus subtilis, 10-13 parts of Rhodopseudomonas palustris, huge
8-12 parts of Bacterium anthracoides, 4-7 parts of lactobacillus acidophilus, 5-8 parts of streptococcus fecalis, 8-11 parts of Bacillus circulans, Candida utilis ferment
7-10 parts female, 4-9 parts of nitrobacteria and 5-8 parts of denitrifying bacteria.
Further, according to parts by weight, 40 parts of acetic acid, 60 parts of ethyl alcohol, sucrose water 180 are contained in the fermentation liquid base fluid
Part, 20 parts of magnesium sulfate, 35 parts of potassium dihydrogen phosphate, 75 parts of sodium chloride, 35 parts of sodium carbonate, 240 parts of glucose and 400 parts of distilled water.
A kind of preparation method of complex micro organism fungicide that improveing aquaculture system, by being added hybrid bacterial strain to hair
Fermentation obtains in zymotic fluid base fluid.
The microbial inoculum is that ostreae testa pulverata, biological peptide and freeze drying protectant are added in hybrid bacterial strain fermentation liquid, is centrifuged and receives after mixing
Collection precipitating, obtains the complex micro organism fungicide；Wherein, the quality of ostreae testa pulverata, biological peptide, freeze drying protectant and the fermentation liquid
Than for (1~10): (0.1~5): (10~20): 100.
Further:
(1) according to each component weight in the fermentation liquid base fluid, each base-material is weighed respectively, is sufficiently dissolved, and pH to 7.0 is adjusted,
It is spare that fermentation liquid base fluid is made in high pressure sterilization；
(2) according to parts by weight, 8-12 parts of bacillus subtilis, 10-13 parts of Rhodopseudomonas palustris, bacillus megaterium are taken
8-12 parts, 4-7 parts of lactobacillus acidophilus, 5-8 parts of streptococcus fecalis, 8-11 parts of Bacillus circulans, 7-10 parts of candida utili, nitre
Change bacterium 4-9 parts and denitrifying bacteria 5-8 parts, each strain is seeded to fermentation base fluid illumination cultivation 3-7 at 30-37 DEG C respectively
It；
(3) each bacterium solution obtained in step (2) is switched in fermentation base fluid again and expands culture, in 30-37
Illumination cultivation 5-8 days at DEG C, until total strain density in fermentation liquid reaches (1~10) × 108Stop fermentation when a/L；
(4) ostreae testa pulverata, biological peptide and freeze drying protectant are added in the fermentation liquid of Xiang Suoshu, is thoroughly mixed 8-12h, from
Scheming centrifugation, removes supernatant, will precipitate vacuum freeze drying, obtain the complex micro organism fungicide.
The freeze drying protectant is the glycerite and aqueous trehalose for being in mass ratio 1-3:1-2, wherein glycerite
Concentration is 30%, algae sugar juice concentration is 10%；Wherein, glycerite, aqueous trehalose are commercial products.
A kind of preparation method of complex micro organism fungicide that improveing aquaculture system, the microbial inoculum is as aquaculture water
The application in growth accelerator that the fungicide of body, the cleanser of breeding water body or cultivation are used as.
The beneficial effects of the present invention are:
Include in complex micro organism fungicide in the present invention it is quick the microorganism of dissolved oxygen is provided, and containing water can be decomposed
The microorganism of ammonia nitrogen, hydrogen sulfide and nitrite in body, while also containing nutrition needed for growth and development can be provided for aquatic livestock
The bacterial strain of substance.Have complementary advantages between each bacterial strain, collaboration plays a role；It quickly can breed to be formed after being further applied to water body
Dominant population, macromolecular organic matter, residual bait excrement in fast decoupled aquaculture system etc., ammonia nitrogen, phosphorus in water body of degrading,
The substances such as nitrite, hydrogen sulfide, purify water, and improve water body dissolved oxygen content, and can remove pathogenic bacteria, such as pathogenic large intestine
Bacillus, mould, staphylococcus aureus, Candida albicans etc..The composite bacteria agent is with good stability and high efficiency, can be strong
Change aquatic animal constitution and promote Animal performance, reduces the death rate in breeding process.And the preparation side of the microbial bacterial agent
Method is simple, and production cost is low.
Specific embodiment:
Technical solution of the present invention is further elaborated below with reference to embodiment, described embodiment is only this hair
Bright a part of the embodiment, but institute's protection scope of the present invention is without being limited thereto, based on the embodiments of the present invention, ordinary skill
Personnel's obtained all other embodiment without making creative work belongs to what the present invention was protected
Range.
Embodiment 1: it is a kind of improve aquaculture system complex micro organism fungicide preparation method, as follows into
Row:
(1) according to each component weight in the fermentation liquid base fluid, 30 grams of fermentation liquid base fluid base-material acetic acid, ethyl alcohol are weighed respectively
50 grams, 150 grams of sucrose water, 10 grams of magnesium sulfate, 20 grams of potassium dihydrogen phosphate, 50 grams of sodium chloride, 20 grams of sodium carbonate, 150 grams of glucose
With 300 grams of distilled water.Sufficiently dissolution, adjusts pH to 7.0, and it is spare that fermentation liquid base fluid is made in high pressure sterilization；
(2) 8 grams of bacillus subtilis, 10 grams of Rhodopseudomonas palustris, huge bud are taken according to parts by weight according to parts by weight
8 grams of spore bacillus, 4 grams of lactobacillus acidophilus, 5 grams of streptococcus fecalis, 8 grams of Bacillus circulans, 7 grams of candida utili, nitrobacteria 4
Gram and 5 grams of denitrifying bacteria, by each strain be in mass ratio 3% inoculum concentration be seeded to fermentation base fluid at 30-32 DEG C illumination train
After supporting 3-7 days each bacterium solution is forwarded in fermentation base fluid for the inoculation of 3% inoculum concentration in mass ratio again and expands culture, in 30-32
Illumination cultivation 5-8 days at DEG C, until total strain density in fermentation liquid reaches (8~10) × 108Stop fermentation when a/L.
(3) mass ratio of ostreae testa pulverata, corn peptide, freeze drying protectant and the fermentation liquid is 3:1:10:100, wherein being lyophilized
Protective agent is that mass fraction is 30% glycerite (as 30g glycerol is dissolved in 70g water) and 10% aqueous trehalose, the two matter
Amount is than being 3:1.It is thoroughly mixed 10h, 4000rpm/min centrifugation removes supernatant, precipitating pressed -50 DEG C of pre-freeze 5h, gradually
It is warming up to the dry total 12h of 30 DEG C of trunks and carries out vacuum freeze drying, obtain the complex micro organism fungicide.
Embodiment 2:
Prepared by the step of complex micro organism fungicide is recorded by embodiment 1, difference from Example 1 is:
(1) according to each component weight in the fermentation liquid base fluid, 40 grams of fermentation liquid base fluid base-material acetic acid, ethyl alcohol are weighed respectively
60 grams, 180 grams of sucrose water, 20 grams of magnesium sulfate, 35 grams of potassium dihydrogen phosphate, 75 grams of sodium chloride, 35 grams of sodium carbonate, 240 grams of glucose
With 400 grams of distilled water.Sufficiently dissolution, adjusts pH to 7.0, and it is spare that fermentation liquid base fluid is made in high pressure sterilization；
(2) 10 grams of bacillus subtilis, 12 grams of Rhodopseudomonas palustris, huge bud are taken according to parts by weight according to parts by weight
10 grams of spore bacillus, 5 grams of lactobacillus acidophilus, 7 grams of streptococcus fecalis, 9 grams of Bacillus circulans, 8 grams of candida utili, nitrobacteria
7 grams and 6 grams of denitrifying bacteria.
(3) mass ratio of ostreae testa pulverata, corn peptide, freeze drying protectant and the fermentation liquid is that 5:3:15:100 (use by embodiment
Specific point value)
Embodiment 3:
Prepared by the step of complex micro organism fungicide is recorded by embodiment 1, difference from Example 1 is:
(1) according to each component weight in the fermentation liquid base fluid, 50 grams of fermentation liquid base fluid base-material acetic acid, ethyl alcohol are weighed respectively
70 grams, 200 grams of sucrose water, 30 grams of magnesium sulfate, 50 grams of potassium dihydrogen phosphate, 100 grams of sodium chloride, 50 grams of sodium carbonate, 300 grams of glucose
With 500 grams of distilled water.Sufficiently dissolution, adjusts pH to 7.0, and it is spare that fermentation liquid base fluid is made in high pressure sterilization；
(2) 12 grams of bacillus subtilis, 13 grams of Rhodopseudomonas palustris, huge bud are taken according to parts by weight according to parts by weight
12 grams of spore bacillus, 7 grams of lactobacillus acidophilus, 8 grams of streptococcus fecalis, 11 grams of Bacillus circulans, 10 grams of candida utili, nitrification are thin
9 grams and 8 grams of denitrifying bacteria of bacterium.
(3) mass ratio of ostreae testa pulverata, corn peptide, freeze drying protectant and the fermentation liquid is 9:5:20:100.
Embodiment 4: the aquatic livestock specific complex microbial bacterial agent prepared using this method is chosen two 20 mu of prawns and supported
Pond is grown, 2 microbial inoculum of above-described embodiment is thrown in a wherein pond and carries out application test, wherein dosage 1kg；It is another
It is a not add as control, test result such as table 1:
Table 1: aquatic livestock application test
Test group compared with the control group, the COD (COD) in test group pond, ammonia nitrogen (NH4+-N), nitroso
The degradation effect of nitrogen (NO2-N) is obvious, and after complex micro organism fungicide is launched, the deenergized period of product is 1-2 months, launches 1
After month, the indices of aquaculture pond water body are stablized, and water quality is good.Beneficial microbe in complex micro organism fungicide is in life
Metabolism generates the substance for facilitating aquatic livestock growth in growth process, and effect not only makes to be greatly improved in terms of water quality, more
The autoimmunity and intestinal microenvironment of aquatic livestock further are enhanced, and inhibits the numerous of pathogenic microorganism in water body
It grows, wherein test group reduces 54% than the disease incidence of control group prawn, and prawn yield improves 30% relatively, improves
The economic benefit of aquaculture.
Above-described embodiment is obtained complex micro organism fungicide simultaneously to be applied in breeding water body, similarly there is above-mentioned effect
Fruit.
Comparative example 1: the microbial bacterial agent used for aquiculture of certain Biotechnology Co., Ltd production
Method: taking 5 parts of aquaculture system 2L, is separately added into above-described embodiment 2 and certain Biotechnology Co., Ltd produces
Microbial bacterial agent used for aquiculture (mainly contain bacillus subtilis, bacillus licheniformis) 2g, every three days plus microbial inoculum is primary, even
It is continuous to feed intake after three weeks, measurement result.
Each component content table of water body such as table 1 before testing
Table 1
| COD(mg/L) | NH4 +-N(mg/L) | Total nitrogen (mg/L) | Total phosphorus (mg/L) | NO2 --N(mg/L) |
| 193 | 9.5 | 17.4 | 2.07 | 1.88 |
Water body after microbial bacterial agent is added to detect concrete condition such as table 2
Table 2
By above-mentioned comparison it is found that present invention microbial bacterial agent produced, the water pollution for effectively solving aquaculture are asked
Topic, and it is not likely to produce secondary pollution.
Comparative example 2: the microbial bacterial agent used for aquiculture of certain Biotechnology Co., Ltd production
Method: choosing 4 20 mu of prawn culturing ponds, applies embodiment 2 and comparative example (the limited public affairs of certain biotechnology respectively
Take charge of production microbial bacterial agent used for aquiculture, contain bacillus subtilis, bacillus licheniformis) prepared by microbial bacterial agent
1000g, one of them does not add any microbial bacterial agent, and application microbial inoculum is primary within every 15 days, after continuous dosing March, measurement knot
Fruit.
| Detection project | Embodiment 2 | Comparative example | It is not added with microbial inoculum group |
| Average every prawn weight g | 30.4 | 24.7 | 20.3 |
By above-mentioned comparison it is found that present invention microbial bacterial agent produced, improves the upgrowth situation of aquatic livestock, improve
The yield and quality of aquatic products.It is a kind of safe and efficient microbial bacterial agent used for aquiculture, is worthy to be popularized and uses.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of complex micro organism fungicide for improveing aquaculture system, it is characterised in that: microbial inoculum is bacillus subtilis, marsh
Red pseudomonas, bacillus megaterium, lactobacillus acidophilus, streptococcus fecalis, Bacillus circulans, candida utili, nitrification are thin
Bacterium and denitrifying bacteria hybrid bacterial strain tunning.
2. the complex micro organism fungicide of improvement aquaculture system according to claim 1, it is characterised in that: the microbial inoculum is
Ostreae testa pulverata, biological peptide and freeze drying protectant are added in hybrid bacterial strain fermentation liquid, precipitating is collected by centrifugation after mixing, obtains described compound
Microbial bacterial agent；Wherein, the mass ratio of ostreae testa pulverata, biological peptide, freeze drying protectant and the fermentation liquid is (1~10): (0.1~
5): (10~20): 100.
3. the complex micro organism fungicide of improvement aquaculture system as described in claim 2, it is characterised in that: the freeze-drying is protected
Protecting agent is the glycerite and aqueous trehalose for being in mass ratio 1-3:1-2, wherein glycerite concentration is that 30% trehalose is molten
Liquid concentration is 10%.
4. the complex micro organism fungicide of improvement aquaculture system as described in claim 1 or 2, it is characterised in that: the hair
Zymotic fluid is illumination cultivation 5-8 days at 30-37 DEG C by the inoculum concentration of 3%-5% in fermentation liquid base fluid by hybrid bacterial strain, until hair
Total strain density in zymotic fluid reaches (1~10) × 108Stop fermentation when a/L；Wherein, fermentation liquid base fluid according to parts by weight, second
30-50 parts sour, 50-70 parts of ethyl alcohol, 150-200 parts of sucrose water, 10-30 parts of magnesium sulfate, 20-50 parts of potassium dihydrogen phosphate, sodium chloride
50-100 parts, 20-50 parts of sodium carbonate, 150-300 parts of glucose and 300-500 parts of distilled water.
5. the complex micro organism fungicide of improvement aquaculture system according to claim 4, it is characterised in that: the Mixed Microbes
Strain is according to parts by weight, 8-12 parts of bacillus subtilis, 10-13 parts of Rhodopseudomonas palustris, 8-12 parts of bacillus megaterium, thermophilic
4-7 parts of Lactobacillus lactis, 5-8 parts of streptococcus fecalis, 8-11 parts of Bacillus circulans, 7-10 parts of candida utili, nitrobacteria 4-9
Part and denitrifying bacteria 5-8 parts.
6. a kind of preparation method of the complex micro organism fungicide of improvement aquaculture system described in claim 1, feature exist
In: it is obtained by the way that hybrid bacterial strain is added to ferment into fermentation liquid base fluid.
7. the preparation method of the complex micro organism fungicide of improvement aquaculture system according to claim 6, it is characterised in that:
The microbial inoculum is that ostreae testa pulverata, biological peptide and freeze drying protectant are added in hybrid bacterial strain fermentation liquid, and precipitating is collected by centrifugation after mixing, obtains
To the complex micro organism fungicide；Wherein, the mass ratio of ostreae testa pulverata, biological peptide, freeze drying protectant and the fermentation liquid be (1~
10): (0.1~5): (10~20): 100.
8. by the preparation method of the complex micro organism fungicide of improvement aquaculture system described in claim 6 or 7, feature exists
In: (1) according in fermentation liquid base fluid each component weight, each base-material is weighed respectively, is sufficiently dissolved, and adjusts pH to 7.0, high pressure is gone out
It is spare that fermentation liquid base fluid is made in bacterium；
(2) according to parts by weight, 8-12 parts of bacillus subtilis, 10-13 parts of Rhodopseudomonas palustris, bacillus megaterium 8-12 are taken
Part, 4-7 parts of lactobacillus acidophilus, 5-8 parts of streptococcus fecalis, 8-11 parts of Bacillus circulans, 7-10 parts of candida utili, nitrification are thin
4-9 parts and denitrifying bacteria 5-8 parts of bacterium, each strain is seeded to fermentation base fluid respectively illumination cultivation 3-7 days at 30-37 DEG C；
(3) each bacterium solution obtained in step (2) is switched in fermentation base fluid again and expands culture, at 30-37 DEG C
Illumination cultivation 5-8 days, until total strain density in fermentation liquid reaches (1~10) × 108Stop fermentation when a/L；
(4) ostreae testa pulverata, biological peptide and freeze drying protectant are added in the fermentation liquid of Xiang Suoshu, is thoroughly mixed 8-12h, centrifuge
Centrifugation removes supernatant, will precipitate vacuum freeze drying, obtain the complex micro organism fungicide.
9. the preparation method of the complex micro organism fungicide of improvement aquaculture system according to claim 8, it is characterised in that:
The freeze drying protectant is the glycerite and aqueous trehalose for being in mass ratio 1-3:1-2, wherein glycerite concentration is
30% aqueous trehalose concentration is 10%.
10. a kind of preparation method of the complex micro organism fungicide of improvement aquaculture system described in claim 1, feature exist
In: in the growth accelerator that the microbial inoculum is used as the fungicide of aquaculture system, the cleanser of breeding water body or cultivation
Using.
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