CN110343690B - 脱羧酶多肽及其在制备酪胺和多巴胺中的应用 - Google Patents

脱羧酶多肽及其在制备酪胺和多巴胺中的应用 Download PDF

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CN110343690B
CN110343690B CN201810302720.1A CN201810302720A CN110343690B CN 110343690 B CN110343690 B CN 110343690B CN 201810302720 A CN201810302720 A CN 201810302720A CN 110343690 B CN110343690 B CN 110343690B
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陈海滨
黄勇开
蔡宝琴
徐青
张城孝
胡东晓
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Enzymaster Ningbo Bio Engineering Co Ltd
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Abstract

本公开内容提供了可用于在工业相关条件下催化L‑酪氨酸脱羧生成酪胺或催化左旋多巴脱羧生成多巴胺的工程化脱羧酶的氨基酸序列、制备方法及其反应工艺。本公开内容还提供了编码工程化脱羧酶多肽的多核苷酸序列、能够表达工程化脱羧酶多肽的重组工程菌和使用该重组工程菌生产酪胺或多巴胺的方法。与其他制备方法相比，本发明提供的工程化脱羧酶多肽的活性和稳定性更好，大大降低了酪胺或多巴胺的生产成本，具有很好的工业应用前景。

Description

脱羧酶多肽及其在制备酪胺和多巴胺中的应用
技术领域
本发明属于生物工程技术领域，具体涉及一种生产酪胺和多巴胺的脱羧酶多肽的序列、制备方法及其反应工艺。
背景技术
酪胺又名对羟基苯乙胺，在自然界中存在于一些寄生树及腐败的动物组织中；多巴胺是一种神经传导物质，用来帮助细胞传送脉冲的化学物质。酪胺和多巴胺作为一种重要的生物胺，具有很大的生理功能和医药价值。在人体内，酪胺具有促进去肾上腺素分泌、收缩末梢神经及升高血压的生理作用；多巴胺具有增强心肌收缩力，增加排血量，加快心率等作用。在医药化工领域，酪胺是许多药物如多巴胺、降血脂药苯扎贝特的合成前体；多巴胺则作为多巴司他特和雷福明等药物的前体。此外，酪胺还被广泛的用于生物医用材料的制备，如透明质酸-酪胺水凝胶，聚乙烯醇-酪胺水凝胶、生物素-酪胺轭合物以及抗体纯化介质等。
现有关于酪胺合成技术基本为化学合成法，如苯乙腈法、茴香醛法、对羟基苯甲醛法、苯酚法。多巴胺的合成主要采用的是香兰素法。这些方法普遍有步骤多、对设备要求高、对环境污染大的缺点，对于工业化生产来说也有相当难度。近年来，随着人们环保意识的增强，绿色工艺的生物酶法日益受到重视。酶促反应具有立体选择性强、反应条件温和、催化效率高等优点，表现出良好的工业运用前景。因此，酶促反应已经成为合成酪胺和多巴胺的重要研究领域。
目前具有可行性的酶促反应是使用脱羧酶作用于L-酪氨酸或左旋多巴，分别形成酪胺或多巴胺。对酪氨酸脱羧酶的研究发现，野生菌的产酶能力低下，且酶的活性或稳定性无法满足工业生产的需要。目前用脱羧酶作用于左旋多巴来合成多巴胺的方法还未见报道。
发明内容
1概述
为了解决当前生产酪胺的生产工艺中存在的问题，本发明提供了一种经济、高效的酶促反应的解决方案，使用本发明方法制备的产物浓度高，反应条件温和，对环境友好，操作简便，易于工业放大，因此具有很好的工业应用前景。
本发明的第一方面提供了一种新型的工程化脱羧酶多肽。这种脱羧酶多肽是由一种性能不高的野生型的脱羧酶经过人为的定向进化过程的改造，发生一定数量的氨基酸残基置换、***或缺失等突变而来。野生型的脱羧酶源自于嗜热链球菌(Streptococcusthermophilus)，其序列如SEQ ID No 2所示。经发明人检测，SEQ ID No 2所对应的蛋白具有脱羧酶活性，可以催化L-酪氨酸脱羧生成酪胺(图1)，也可以催化左旋多巴脱羧生成多巴胺(图2)。然而，SEQ ID No 2所对应的蛋白酶的稳定性不够好，即使是在低温条件下(-20℃到4℃)放置，SEQ ID No 2所对应的蛋白酶的活性有显著的损失，这使得该酶在被微生物表达出来后，难以被储藏或进一步加工(例如做成固定化酶)。经发明人检测，如实施例2所述方法制备的含SEQ ID No 2所对应蛋白的酶液，在-20℃下放置48小时候后，其催化图1或图2所示反应的活性会下降约30％；在4℃下放置48小时候后，其催化图1或图2所示反应的活性会下降约50％；在30℃下放置48小时候后，其催化图1或图2所示反应的活性会下降约70％。为了提高其稳定性，发明人开发了一系列稳定性和/或活性更好的工程化脱羧酶多肽。这种工程化脱羧酶多肽是经过人为的定向进化过程的改造，发生一定数量的氨基酸残基置换、***或缺失等突变而来。
本发明提供的改进的工程化脱羧酶多肽与SEQ ID NO：2对应的野生型的脱羧酶相比，具有更高的稳定性和/或活性，能够更高效地催化L-酪氨酸脱羧生成酪胺，也可以更高效地催化左旋多巴脱羧生成多巴胺。在一些实施方案中，本公开内容中改进的工程化脱羧酶多肽能够以至少等于或大于SEQ ID No：2的活性和/或稳定性将L-酪氨酸转化为酪胺和二氧化碳。在一些实施方案中，本公开内容中改进的工程化脱羧酶多肽能够以至少等于或大于SEQ ID No：2的稳定性和/或活性将左旋多巴转化为多巴胺和二氧化碳。
本发明中改进的脱羧酶多肽可包括在对应以下的残基位置与SEQ ID NO：2序列相比的一个或多个残基差异的氨基酸序列：X12，X26，X30，X44，X51，X52，X56，X58，X63，X72，X123，X133，X147，X181，X210，X271，X294，X317，X376，X380，X388，X454，X465，X467，X517，X530，X535，X536，X538，X576。改进的脱羧酶多肽包括包含以下特征至少之一的氨基酸序列：D12Y，D12T，Q26T，Q26F，D30E，Q44R，Q44K，Q44I，Q44L，K51P，K51T，P52T，P52V，P52S，A56S，A56T，A56P，Q58R，Q58V，Q58H，E63D，K72R，A123S，M133V，Y147F，A181E，N210L，I271P，I271S，V294A，V317E，A376S，F380L，I388T，K454R，K465R，K465V，K465L，K465G，V467L，N517D，N530D，V535W，T536G，S538V，E576K；或同时在这些差异的基础上，包含1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、18、20、21、22、23、24、25或更多个氨基酸残基的***或缺失。
更具体的，在一些实施方案中，在SEQ ID NO：2基础上改进的工程化脱羧酶多肽包括对应SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104所示氨基酸序列组成的多肽。
在一些实施方案中，改进的工程化脱羧酶多肽包括与SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104的参考序列至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多序列同一性的氨基酸序列。
两条氨基酸序列或两条核苷酸序列之间的同一性均可通过本领域常用的算法得到，可采用NCBI Blastp和Blastn软件根据默认参数计算得到，也可采用采用Clustal W算法(Nucleic Acid Research,22(22):4673-4680,1994)。
在另一方面，本发明提供了编码工程化脱羧酶多肽的多核苷酸序列。在一些实施方案中，多核苷酸可以是具有用于表达工程化脱羧酶多肽的一种或多种控制序列的表达载体的部分。在一些实施方案中，多核苷酸可包括对应SEQ ID No：3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103所示序列的多核苷酸序列。
如本领域技术人员所知，由于核苷酸密码子的简并性，编码SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104的氨基酸序列的多核苷酸序列不仅仅局限于SEQ ID No：3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103。本发明的脱羧酶基因的核酸序列也可以是编码序列表中SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104所示氨基酸序列的其他任何核酸序列。
在另一方面，本公开内容提供包含编码工程化脱羧酶的多核苷酸或能够表达工程化脱羧酶的表达载体和宿主细胞。在一些实施方案中，宿主细胞可以是细菌宿主细胞，比如大肠杆菌。宿主细胞可用于表达和分离本文所述的工程化脱羧酶，或可选地直接用于反应转化底物为产物。
在一些实施方案中，以完整细胞、粗提取物、分离的多肽或纯化的多肽形式的工程化脱羧酶可单独使用，或以固定化形式(比如固定在树脂上)使用。
本公开内容还提供了使用本文公开的工程化脱羧酶多肽分解结构式(II)所示的氨基酸来制备结构式(I)所示的氨基化合物的方法：
Figure BDA0001620215180000041
其中R是任选地取代的C1-C8烃基，或是任选地取代的芳基或杂芳基；所述方法包括在合适的反应条件下，将结构式(II)的氨基酸底物与脱羧酶多肽接触，其中所述脱羧酶多肽是本文所述的工程化脱羧酶多肽。在一些实施方案中，所述工程化脱羧酶多肽与SEQ IDNO：2具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列同一性并能够以与SEQ ID NO：2相比更高的转化率将结构式(II)所示的化合物转化为结构式(I)所示的化合物。
在一些实施方案中，改进的工程化脱羧酶多肽可以用在制备酪胺的方法中：
Figure BDA0001620215180000042
在这些实施方案中，所述方法包括在合适的反应条件下，将结构式A1所示化合物：
Figure BDA0001620215180000043
与本文公开的改进的工程化脱羧酶多肽接触的步骤。
在一些实施方案中，改进的工程化脱羧酶多肽可以用在制备多巴胺的方法中：
Figure BDA0001620215180000051
在这些实施方案中，所述方法包括在合适的反应条件下，将结构式A2所示化合物：
Figure BDA0001620215180000052
与本文公开的改进的工程化脱羧酶多肽接触的步骤。
用于该方法的工程化的脱羧酶多肽的具体实施方案在详述中进一步提供。可用在以上方法中的改进的工程化脱羧酶多肽可包括选自对应SEQ ID NO：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104的氨基酸序列。
如本文公开的使用工程化多肽用于制备式(I)化合物、酪胺或多巴胺的方法中的任一种可在一系列合适的反应条件下被执行，所述一系列合适的反应条件包括但不限于pH、温度、缓冲液、溶剂***、底物载量、多肽载量、压力和反应时间的范围。例如，在一些实施方案中，制备式(I)化合物、酪胺或多巴胺的方法可被执行，其中合适的反应条件包括：(a)约5g/L至400g/L底物化合物(例如，化合物(II)、A1或A2)的底物载量；(b)约0.5g/L至10g/L工程化多肽，或1g/L至100g/L表达了工程化多肽的湿菌体；(c)约0％(v/v)至约60％(v/v)的有机溶剂，这里所述的有机溶剂包括但不限于二甲基亚砜(DMSO)、二甲基甲酰胺(DMF)、乙酸异丙酯、甲醇、乙醇、丙醇或异丙醇(IPA)；(d)约0.01mM至5mM的PLP辅因子浓度；(e)约3.0至约8.0的pH；和(f)约10℃至60℃的温度。
2详述
2.1定义
关于本公开内容，除非另外明确定义，否则本文说明书中使用的技术术语和科学术语具有本领域普通技术人员通常理解的含义。
“蛋白”、“多肽”和“肽”在本文可互换使用，表示由酰胺键共价连接的至少两个氨基酸的聚合物，而不论长度或翻译后修饰(如，糖基化、磷酸化、脂质化、豆蔻酰化、泛素化等等)。该定义包括D-氨基酸和L-氨基酸、以及D-氨基酸和L-氨基酸的混合物。
“工程化脱羧酶”、“工程化脱羧酶多肽”、“改进的脱羧酶多肽”和“工程化多肽”在本文可互换使用。
“菌体”或“湿菌体”指表达了多肽或工程化多肽的宿主细胞，包括实施例2和实施例9所示的制备过程所得到的湿菌体。
“多核苷酸”和“核酸”在本文可互换使用。
如本文使用的“辅因子”指在催化反应中与酶联合起作用的非蛋白化合物。如本文使用的，“辅因子”意图包括维生素B6家族化合物PLP、PN、PL、PM、PNP和PMP，其有时也被称作辅酶。
“磷酸吡哆醛”、“PLP”、“吡哆醛5’-磷酸”、“PYP”和“P5P”在本文可互换地使用，来指在脱羧酶反应中用作辅因子的化合物。
“编码序列”指编码蛋白的氨基酸序列的核酸部分(例如，基因)。
“天然存在的”或“野生型”是指在自然界发现的形式。例如，天然存在的或野生型的多肽或多核苷酸序列是存在于生物体中、可分离自自然界中的来源且未通过人工操作有意地修饰的序列。
“重组的”或“工程化的”或“非天然存在的”当用于指例如细胞、核酸或多肽时，是指如下材料或与该材料的天然形式或固有形式相对应的材料：所述材料以自然界中不会存在的方式被改变，或与其相同但是从合成材料和/或通过使用重组技术操作而产生或获取。
“序列同一性”和“同源性”在本文可互换地用于指多核苷酸之间或多肽之间的对比(“序列同一性”和“同源性”通常以百分比的形式来表示)，且通过在比较窗口上比较两个最佳对齐的序列来确定，其中多核苷酸或多肽序列在比较窗口中的部分与参照序列相比可包括添加或缺失(即，空位)，用于两个序列的最佳对齐。百分比可以通过如下计算：确定两个序列中出现相同的核酸碱基或氨基酸残基的位置数目以产生匹配位置的数目，将匹配位置的数目除以比较窗口中位置总数并将结果乘以100以得到序列同一性百分比。可选地，该百分比可通过以下计算：确定相同核酸碱基或氨基酸残基在两个序列中都存在的位置数或核酸碱基或氨基酸残基与空位对齐的位置数以得到匹配位置数，将该匹配位置数除以比较窗口中的位置总数，并将结果乘以100以得到序列同一性的百分比。本领域技术人员将认识到，存在许多可用于比对两个序列的建立的算法。用于比较的序列最佳比对可例如通过Smith和Waterman，1981，Adv.Appl.Math.2:482的局部同源性算法、通过Needleman和Wunsch，1970，J.Mol.Biol.48:443的同源性比对算法，通过Pearson和Lipman,1988,Proc.Natl.Acad.Sci.USA85:2444的相似性搜索方法，通过这些算法的计算机实现(GCGWisconsin软件包中的GAP、BESTFIT、FASTA或TFASTA)或通过直观检查(一般参见，Current Protocols in MolecuLar Biology,F.M.Ausubel等编著，Current Protocols,Greene Publishing Associates Inc.和John Wiley&Sons,Inc.之间的合资企业,(1995年增刊)(Ausubel))。适宜于确定序列同一性和序列相似性百分比的算法的实例是BLAST和BLAST2.0算法，它们分别描述于AltschuL等人，1990，J.Mol.Biol.215:403-410和AltschuL等,1977,Nucleic Acids Res.3389-3402中。用于执行BLAST分析的软件是通过美国国家生物技术信息中心(National Center for Biotechnology Information)网站公开可用的。该算法包括首先通过鉴定查询序列中长度W的短字来鉴定高评分序列对(HSP)，所述短字与数据库序列中相同长度的字比对时匹配或满足一些正值的阀值得分T。T被称作邻近字评分阈值(AltschuL等，如上述)。这些初始相邻字匹配(word hit)充当用于启始搜索的种子来寻找包含它们的更长的HSP。然后字匹配沿着每个序列在两个方向延伸到累积比对得分不能够增加的程度。对于核苷酸序列，累积得分使用参数M(对于匹配残基对的奖励得分；永远＞0)和N(对于错配残基的惩罚得分；永远＜0)计算。对于氨基酸序列，得分矩阵被用于计算累计得分。当以下情况时，每个方向中的字匹配字串的延伸被终止：累积比对得分从其最大达到值下降了量X；由于累积一个或多个负得分残基比对，累积得分达到0或以下；或到达任一序列末端。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(用于核苷酸序列)使用字长(W)11、期望值(E)10、M＝5、N＝-4以及两链的比较作为默认值。对于氨基酸序列，BLASTP程序使用以下作为缺省值：字长(W)为3，期望值(E)为10和BLOSUM62得分矩阵(见Henikoff和Henikoff,1989,Proc Natl Acad Sci USA89：10915)。序列比对和序列同一性％的示例性确定可使用GCG Wisconsin软件包(Accelrys,Madison WI)中的BESTFIT或GAP程序，使用所提供的缺省参数。
“参考序列”是指用作序列比较的基础的限定序列。参考序列可以是较大序列的子集，例如，全长基因或多肽序列的片段。一般而言，参考序列为至少20个核苷酸或氨基酸残基长，至少25个残基长，至少50个残基长，或者核酸或多肽的全长。因为两种多核苷酸或多肽可以各自(1)包括在两种序列之间相似的序列(即，完整序列的一部分)，且(2)可以进一步包括在两种序列之间不同的序列，两种(或更多)多核苷酸或多肽之间的序列比较通常通过在“比较窗口”内比较两种多核苷酸或多肽的序列来进行以鉴定和比较序列相似性的局部区域。在一些实施方案中，“参照序列”不意为限于野生型序列，且可包括工程化或改变的序列。例如，“在对应于X39的残基处具有亮氨酸的基于SEQ ID NO:2的参考序列”指其中在SEQ ID NO:2中的X39处相应的残基(是脯氨酸)已经改变成亮氨酸的参考序列。
“比较窗口”指至少约20个相邻核苷酸位置或者氨基酸残基的概念性片段，其中序列可以与至少20个相邻核苷酸或氨基酸的参考序列相比较，并且其中比较窗口中的序列的部分可以包括与参考序列(其不包括添加或缺失)相比20％或更少的添加或缺失(即，空位)，用于两序列的最佳比对。比较窗口可以长于20个相邻残基，并且包括任选地30、40、50、100或更长的窗口。
在用于指定的氨基酸或多核苷酸序列的编号的情况下，"相应于"、"参考于"或"相对于"指当指定的氨基酸或多核苷酸序列与参考序列相比较时指定的参考序列残基的编号。换言之，给定序列的残基编号或残基位置是根据参考序列指定的，而不是给定氨基酸或多核苷酸序列内的残基的实际数字位置指定的。例如，可以将给定的氨基酸序列诸如工程化脱羧酶的氨基酸序列与参考序列进行比对，这是通过引入空位以优化这两条序列之间的残基匹配而实现的。在这些情况下，虽然存在空位，但是给定氨基酸或多核苷酸序列中的残基编号相对于已与其比对的参考序列制定。
“氨基酸差异”或“残基差异”指在多肽序列的一个位置处氨基酸残基相对于参考序列中相应位置处的氨基酸残基的差异。本文中氨基酸差异的位置一般被称为“Xn”，其中n指残基差异基于其的参考序列中的相应位置。例如，“与SEQ ID NO:2相比在位置X44处的残基差异”指在相应于SEQ ID NO:2的位置44的多肽位置处的氨基酸残基的差异。因此，如果SEQ ID NO:2的参考多肽在位置44处具有谷氨酰胺，那么“与SEQ ID NO:2相比在位置X44处的残基差异”是指在相应于SEQ ID NO:2的位置44的多肽位置处除了谷氨酰胺之外的任何残基的氨基酸取代。在本文的大多数实例中，在一个位置处的特定氨基酸残基差异表示为“XnY”，其中“Xn”指如以上描述的对应位置，并且“Y”为在工程化多肽中发现的氨基酸的一字母标识符(即，与参考多肽中的不同的残基)。在一些实例中(例如，在表1中)，本公开内容还提供由常规符号“AnB”表示的特定氨基酸差异，其中A为参考序列中的残基的一字母标识符，“n”为在参考序列中的残基位置的编号，并且B为工程化多肽的序列中残基取代的单字母标识符。在一些实例中，本公开内容的多肽可包含相对于参考序列的一个或更多个氨基酸残基差异，其通过相对于参考序列存在残基差异处的特定位置的列表表示。在一些实施方案中，当多于一个氨基酸可被用于多肽的特定残基位置时，可使用的多个氨基酸残基通过“/”分隔开(例如，X44R/X44I)。
“缺失”指通过从参考多肽去除一个或更多个氨基酸而对多肽的修饰。缺失可以包括除去1个或多个、2个或多个氨基酸，5个或多个氨基酸，10个或多个氨基酸，15个或多个氨基酸，或20个或多个氨基酸，多达组成参照酶的氨基酸总数的10％，或多达组成参照酶的氨基酸总数的20％，同时保留工程化脱羧酶的酶活性和/或保留工程化脱羧酶的改进的性质。缺失可以涉及多肽的内部部分和/或末端部分。在各种实施方案中，缺失可以包括连续的区段或者可以是不连续的。
"***"指通过从参考多肽添加一个或多个氨基酸的多肽的修饰。在一些实施方案中，改进的工程化脱羧酶包括一个或更多个氨基酸***天然存在的脱羧酶多肽，以及一个或更多个氨基酸***其他改进的脱羧酶多肽。可在多肽的内部部分***，或***到羧基或氨基末端。如本文所用的，***包括本领域中已知的融合蛋白。***可以是氨基酸的连续区段，或者被在天然存在的多肽中一个或更多个氨基酸分隔。
如本文所用的"片段"指具有氨基末端和/或羧基末端缺失、但是其中保留的氨基酸序列与序列中相应的位置相同的多肽。片段可以为至少10个氨基酸长、至少20个氨基酸长，至少50个氨基酸长或更长，以及高达全长脱羧酶多肽的70％、80％、90％、95％、98％和99％。
“分离的多肽”是指如下多肽：所述多肽基本上与其天然伴随的其他物质例如蛋白、脂质和多核苷酸分离。该术语包括已从其天然存在的环境或表达***(例如，宿主细胞或体外合成中)移去或纯化的多肽。改进的脱羧酶多肽可以存在于细胞内、存在于细胞培养基中或者以各种形式制备，诸如裂解物或分离的制备物。像这样，在一些实施方案中，改进的脱羧酶多肽可以是分离的多肽。
“改进的酶性质”指与参考脱羧酶相比显示任何酶性质的改进的脱羧酶多肽，所述参考脱羧酶诸如野生型脱羧酶或另一种改进的工程化脱羧酶。期望改进的酶性质包括，但不限于，酶活性(其可以底物的转化百分比的方式被表示)、热稳定性、溶剂稳定性、pH活性特征、辅因子需求、对抑制物的耐受性(例如，底物或产物抑制)、立体特异性和立体选择性(包括对映体选择性或非对映体选择性)。
“转化”指底物向相应的产物的酶转化。“转化百分比”或“转化率”是指在指定条件下在一段时间内被转化为产物的底物的百分比。因此，脱羧酶多肽的“酶活性”或“活性”可以被表示为底物向产物的“转化百分比”。
“热稳定的”指与野生型酶相比脱羧酶多肽在暴露于升高的温度(例如30-60℃)持续一段时间(例如0.5-48小时)之后维持相似活性(例如多于50％)。
“溶剂稳定的”指与野生型酶相比脱羧酶多肽在暴露于不同浓度(例如5-99％)的溶剂(乙醇、异丙醇、二甲基亚砜(DMSO)、四氢呋喃、2-甲基四氢呋喃、丙酮、甲苯、乙酸丁酯、甲基叔丁基醚等)持续一段时间(例如0.5-24小时)之后维持相似的活性(多于例如50％至80％)。
“合适的反应条件”指生物催化反应溶液中的那些条件(例如，酶载量、底物载量、辅因子载量、温度、pH、缓冲液、共溶剂等的范围)，在该条件下本公开内容的脱羧酶多肽能将底物转化成期望的产物化合物。示例性“合适的反应条件”被提供于本公开内容并通过实施例例证。
“烃基”是指直链或支链的烃基团。符号“C”后出现下标的数字规定了特定基团可包含的碳原子数。例如，“C1-C8”是指具有1至8个碳原子的直链或支链烃基基团。烃基基团可任选第被一个或多个取代基基团取代。“芳基”是指6至约20个碳原子的单价芳香族烃基。“杂芳基”和“杂芳族”是指其中母体芳环***的一个或多个碳原子被杂原子(O，N或S)代替的芳基基团。“取代的”当用于修饰指定基团或自由基时，是指指定基团或自由基的一个或多个氢原子各自彼此独立地被相同或不同的取代基代替。“取代的烃基、芳基或杂芳基”指其中一个或多个氢原子用另一取代基代替的烃基、芳基或杂芳基基团。“任选”或“任选地”是指所描述的事件或情况可能发生或不发生；例如“任选地取代的芳基”是指可能被取代或可能未被取代的芳基基团，这一描述包括取代的芳基基团和未取代的芳基基团二者。
本文所用的“化合物”是指伴随本文公开的化合物标明的结构式和/或化学名涵盖的任何化合物。化合物可以其化学结构和/或化学名标明。当化学结构与化学名冲突时，化学结构决定化合物的身份。除非另外具体地描述或指出，否则本文描述的化学结构涵盖所描述化合物的所有可能异构体形式。
2.2改进的工程化脱羧酶
本公开内容提供了可用于在工业相关条件下催化L-酪氨酸脱羧生成酪胺的工程化脱羧酶的氨基酸序列，同时也提供了可用于在工业相关条件下催化左旋多巴脱羧生成多巴胺的工程化脱羧酶的氨基酸序列，还提供了编码工程化脱羧酶多肽的多核苷酸序列。与野生型的脱羧酶相比，本发明提供的工程化脱羧酶多肽的稳定性和/或活性更好，大大降低了酪胺或多巴胺的生产成本，具有很好的工业应用前景。
以下表1举例说明本发明所开发的工程化脱羧酶多肽。每一行给出一个具体的工程化脱羧酶多肽的核苷酸序列编号和氨基酸序列编号，以及与SEQ ID No:2相比较的残基差异。每个举例的工程化脱羧酶多肽的催化性能(综合了活性和稳定性)以“+”表示，具体的含义见表2或表3。
表1
Figure BDA0001620215180000111
Figure BDA0001620215180000121
表2
Figure BDA0001620215180000122
表2所述预处理后的酶液，预处理条件是将酶液放置于30℃下经历48小时(如实施例3所述)，酶液中含有的表1中氨基酸序列对应的脱羧酶多肽的浓度是等量的。酶液中的蛋白浓度约为10g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)。
表3
Figure BDA0001620215180000123
Figure BDA0001620215180000131
表3所述的反应条件中，酶液没有经过预处理，且酶液用量(即5％)比表2所述反应条件中的酶液用量(即20％)要低；酶液中含有的表1中氨基酸序列对应的脱羧酶多肽的浓度是等量的。酶液中的蛋白浓度约为10g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)。
2.3可用于制备工程化脱羧酶多肽的多核苷酸、控制序列、表达载体和宿主细胞
在另一方面，本公开内容提供了编码本文描述的具有脱羧酶活性的工程化多肽的多核苷酸。多核苷酸可与控制基因表达的一种或多种异源调控序列可操作地连接以产生能够表达多肽的重组多核苷酸。包含编码工程化的脱羧酶的异源多核苷酸的表达构建体可被引入合适的宿主细胞以表达相应的工程化脱羧酶多肽。
如对本领域技术人员明显的是，蛋白序列的可得性和相应于多种氨基酸的密码子的知识提供能够编码目标蛋白序列的所有多核苷酸的说明。其中相同氨基酸由可选择的或同义密码子编码的遗传密码的简并性允许产生极大数目的核酸，所有这些核酸编码本文公开的改进的脱羧酶多肽。因此，确定特定的氨基酸序列后，本领域的技术人员可以以不改变蛋白质的氨基酸序列的方式通过仅仅修饰一个或多个密码子的序列来产生任何数目的不同的核酸。在这点上，本公开内容特别地构思了可通过基于可能的密码子选取而选择组合来制备的多核苷酸的各个和每个可能的改变，并且对于本文公开的任何多肽，包括在表1中提供的示例性工程化多肽的氨基酸序列，以及在通过引用并入本文的序列表中作为SEQ IDNO:2-104的偶数序列标识符的序列公开的任何多肽，所有这些改变被认为特别地公开。
在多种实施方案中，密码子被优选地选择以适应在其中产生蛋白的宿主细胞。例如，用于细菌的优选密码子用于表达细菌中的基因；用于酵母中的优选密码子用于在酵母中表达；且用于哺乳动物的优选密码子用于在哺乳动物细胞中表达。
在一些实施方案中，所述多核苷酸编码包含与选自SEQ ID NO:2-104的偶数序列标识符的参考序列至少约80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多序列同一性的氨基酸序列的脱羧酶多肽，其中所述多肽具有脱羧酶活性以及本文描述的改进的特性中的一种或更多种，例如以与SEQ ID NO:2的多肽相比增加的活性将化合物A1或A2转化成酪胺或多巴胺的能力。
在一些实施方案中，所述多核苷酸编码工程化脱羧酶多肽，所述工程化脱羧酶多肽包含与SEQ ID NO:2相比具有以上描述的同一性百分比并且具有选自的一个或更多个氨基酸残基差异的氨基酸序列。在一些实施方案中，本公开内容提供了具有脱羧酶活性的工程化多肽，所述工程化多肽包含与SEQ ID NO:2的参考序列具有至少80％的序列同一性以及具有选自以下位置的残基差异的组合：X12，X26，X30，X44，X51，X52，X56，X58，X63，X72，X123，X133，X147，X181，X210，X271，X294，X317，X376，X380，X388，X454，X465，X467，X517，X530，X535，X536，X538，X576。
在一些实施方案中，编码工程化脱羧酶多肽的多核苷酸包含选自SEQ ID NO:3-103的奇数序列标识符的序列。
在一些实施方案中，所述多核苷酸编码本文所述的多肽，但是在核苷酸水平上与编码工程化脱羧酶的参考多核苷酸具有约50％、55％、60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％或更高的序列同一性。在一些实施方案中，参考多核苷酸序列选自具有SEQ ID NO:3-103的奇数序列标识符的序列。
编码工程化脱羧酶多肽的分离的多核苷酸可以多种方法***作以提供多肽的表达，所述方法包括通过密码子优化来进一步改变序列以改进表达、在合适的有或无另外的控制序列的表达元件中***、和转化入适于表达并产生多肽的宿主细胞。
取决于表达载体，在分离的多核苷酸***载体之前对分离的多核苷酸的操作可以是期望的或必需的。利用重组DNA方法修饰多核苷酸和核酸序列的技术在本领域中是公知的。在以下中提供了指导：Sambrook等人,2001,MolecuLar Cloning:A LaboratoryManual,第三版,Cold Spring Harbor Laboratory Press；和Current Protocols inMolecuLar Biology,Ausubel.F.编著,GreenePub.Associates,1998,2010年更新。
在另一个方面，本公开还涉及重组表达载体，根据它们将被导入的宿主的类型，其包括编码工程化脱羧酶多肽或其变体的多核苷酸，和一个或多个表达调节区，诸如启动子和终止子、复制起点等等。可选地，本公开内容的核酸序列可以通过使核酸序列或包括该序列的核酸构建体***到适当的表达载体中来表达。在产生表达载体时，编码序列位于载体中以使编码序列被可操作地连接于用于表达的合适的控制序列上。
重组表达载体可为任何载体(例如，质粒或病毒)，其可方便地应用于重组DNA步骤中并且可带来多核苷酸序列的表达。载体的选择通常将取决于载体与待引入载体的宿主细胞的相容性。载体可以是线性或闭合环状的质粒。表达载体可以是自主复制的载体，即作为染色体外的实体而存在的载体，它的复制独立于染色体的复制，如质粒、染色体外的元件、微小染色体、或人工染色体。载体可以包含用于保证自我复制的任何工具。可选地，载体可以是在被引入到宿主细胞中时整合到基因组中并且与其所整合到的染色体一起复制的载体。而且，可以使用单一载体或质粒或者一起包含待引入到宿主细胞基因组中的总DNA的两种或多种载体或质粒。
对本公开内容的实施方案有用的很多表达载体是商业上可得的。示例性表达载体可通过将编码改进的脱羧酶多肽的多核苷酸可操作地连接到质粒pACYC-Duet-1(Novagen)中来制备。
在另一方面，本公开内容提供包含编码本公开内容的改进的脱羧酶多肽的多核苷酸的宿主细胞，所述多核苷酸被可操作地连接至在宿主细胞中用于脱羧酶的表达的一个或更多个控制序列。用于表达由本公开内容的表达载体编码的多肽的宿主细胞在本领域是公知的，并且包括但不局限于诸如大肠杆菌、节杆菌属种KNK168、链霉菌属和鼠伤寒沙门氏菌(Salmonella typhimurium)细胞的细菌细胞；诸如酵母细胞(例如，酿酒酵母或巴斯德毕赤氏酵母(Pichia pastoris))的真菌细胞；诸如果蝇S2以及灰翅夜蛾(Spodoptera)Sf9细胞的昆虫细胞；诸如CHO、COS、BHK、293和Bowes黑色素瘤细胞的动物细胞；以及植物细胞。示例性的宿主细胞为大肠杆菌BL21(DE3)。上述宿主细胞可以是野生型的，也可以是经过基因组编辑的工程细胞，比如把宿主细胞基因组中所携带的野生型的脱羧酶基因敲除。上述宿主细胞的合适的培养基以及生长条件在本领域内是公知的。
用于表达脱羧酶的多核苷酸可通过本领域已知的多种方法被引入至细胞。技术包括，除了其他以外，电穿孔、生物颗粒轰击法、脂质体介导的转染、氯化钙转染和原生质体融合。将多核苷酸引入细胞的不同方法对于本领域技术人员是明显的。
2.4产生工程化脱羧酶多肽的方法
通过将编码脱羧酶的多核苷酸经历诱变和/或定向进化方法，可以获得工程化脱羧酶。示例性的定向进化技术可见《Biocatalysis for the Pharmaceutical Industry:Discovery,Development,and Manufacturing》(2009John Wiley&Sons Asia(Pte)Ltd.ISBN:978-0-470-82314-9)。
当工程化多肽的序列为已知时，编码酶的多核苷酸可根据已知的合成方法通过标准的固相方法制备。在一些实施方案中，多达约100个碱基的片段可单独地合成，然后连接(例如，通过酶促或化学的连接方法或聚合酶介导的方法)以形成任何需要的连续序列。例如，本公开内容的多核苷酸和寡核苷酸可通过化学合成制备，使用，例如，描述于Beaucage等人,1981,TetLett22：1859-69的经典的亚磷酰胺方法，或描述于Matthes等人,1984,EMBOJ.3:801-05的方法，例如，如在自动化合成方法中典型地实践的。根据亚磷酰胺方法，寡核苷酸在例如，在自动化DNA合成仪中合成、纯化、退火、连接以及克隆至合适的载体中。另外，基本上任何核酸可从多种商业来源的任一个获得。
在一些实施方案中，本公开内容还提供了用于制备或制作能在合适的反应条件下将化合物A1或A2转化成酪胺或多巴胺的工程化脱羧酶多肽的方法，其中该方法包括在适于表达多肽的培养条件下培养能够表达编码工程化多肽的多核苷酸的宿主细胞，这些宿主细胞可以以菌体的形式直接应用于将化合物A1或A2转化成酪胺或多巴胺的过程中。在一些实施方案中，制备多肽的方法还包括分离多肽。工程化多肽可在合适的细胞中表达，并利用所熟知的用于蛋白纯化的技术中的任何一种或更多种从宿主细胞和/或培养基分离(或回收)，所述用于蛋白纯化的技术包括，除了其他以外，溶菌酶处理、超声、过滤、盐析、超速离心和色谱。
2.5利用工程化脱羧酶的方法以及用其制备的化合物
在另一方面，本文所述的改进的工程化脱羧酶多肽，可将氨基酸的羧基脱去生成氨基化合物。本公开内容还提供了使用本文公开的工程化脱羧酶多肽制备广泛范围的化合物(I)或其结构类似物的方法。在一些实施方案中，工程化脱羧酶多肽可用在制备结构式(I)的化合物的方法中：
Figure BDA0001620215180000161
其中R是任选地取代的C1-C8烃基，或是任选地取代的芳基或杂芳基；所述方法包括在合适的反应条件下，将结构式(II)的氨基酸底物：
Figure BDA0001620215180000162
与脱羧酶多肽接触，其中所述脱羧酶多肽是本文所述的工程化脱羧酶多肽。在一些实施方案中，所述工程化脱羧酶多肽与选自SEQ ID NO:2-104的偶数序列的任一个的参考氨基酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列同一性并能够以与SEQ ID NO：2相比更高的转化率将结构式(II)所示的化合物转化为结构式(I)所示的化合物。
如上所述，可用于本公开内容的方法中的脱羧酶多肽可按照其催化左旋多巴转化为多巴胺的能力或按照其催化L-酪氨酸转化为酪胺的能力来表征。因此，在本文公开的方法的任何实施方案中，可进行该方法，其中脱羧酶多肽能够以与SEQ ID NO：2相比更高的催化性能将左旋多巴转化为多巴胺，并与选自SEQ ID NO:2-104的偶数序列的任一个的参考氨基酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的氨基酸序列同一性。
在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000171
且结构式(II)的底物是：
Figure BDA0001620215180000172
在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000173
且结构式(II)的底物是：
Figure BDA0001620215180000174
在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000175
且结构式(II)的底物是：
Figure BDA0001620215180000181
在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000182
其中R1是-H、-CH2OH、-CH2SH、-CH2SCH3或任选地取代的C1-C4烃基，R2是C1-C4烃基、-H、卤素(如-F、-Cl、-Br和-I)、-NO2、-NO、-SO2R’或-SOR’、-S R’、-N R’R’、-O R’、-CO2R’或-COR’、-C(O)NR’、-SO2NH2或-SO NH2、-CN、CF3，其中每个R’独立地选自-H或(C1-C4)烃基；
且结构式(II)的底物是：
Figure BDA0001620215180000183
在一些实施方案中，R2在苯基环的对位。在一些实施方案中，R2在苯基环的间位。在一些实施方案中，R2在苯基环的邻位。在一些实施方案中，R2同时在苯基环的对位和间位。在一些实施方案中，R2同时在苯基环的对位和邻位。在一些实施方案中，R2同时在苯基环的间位和邻位。
在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000184
其中R3是如以上定义的R2，R2和R1如上定义，且结构式(II)的底物是：
Figure BDA0001620215180000191
在一些实施方案中，R2在苯基环的间位。在一些实施方案中，R2在苯基环的邻位。在该方法的一些实施方案中，结构式(I)的化合物是：
Figure BDA0001620215180000192
且结构式(II)的底物是：
Figure BDA0001620215180000193
在一些实施方案中，改进的工程化脱羧酶多肽可以用在制备酪胺的方法中：
Figure BDA0001620215180000194
在这些实施方案中，所述方法包括在合适的反应条件下，将结构式A1所示化合物：
Figure BDA0001620215180000195
与本文公开的改进的工程化脱羧酶多肽接触的步骤。
在一些实施方案中，改进的工程化脱羧酶多肽可以用在制备多巴胺的方法中：
Figure BDA0001620215180000196
在这些实施方案中，所述方法包括在合适的反应条件下，将结构式A2所示化合物：
Figure BDA0001620215180000201
与本文公开的改进的工程化脱羧酶多肽接触的步骤。
用于该方法的工程化的脱羧酶多肽的具体实施方案在详述中进一步提供。可用在以上方法中的改进的工程化脱羧酶多肽可包括选自对应SEQ ID NO：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104的氨基酸序列。
如本文描述并在实施例中例证的，本公开内容构思了可在本文的方法中使用的合适的反应条件的范围，包括但不限于pH、温度、缓冲液、溶剂***、底物载量、多肽载量和反应时间的范围。用于执行使用本文描述的工程化的脱羧酶多肽将底物化合物生物催化地转化成产物化合物的方法的另外的合适的反应条件可容易地通过常规实验优化，所述常规实验包括但不限于在浓度、pH、温度、溶剂条件的实验反应条件下使工程化脱羧酶多肽与底物化合物接触，并检测产物化合物，例如，利用在本文提供的实施例中描述的方法。
如以上描述的，用于本公开内容的方法的具有脱羧酶活性的工程化多肽通常包含与选自SEQ ID NO:4-104的偶数序列的任一个的参考氨基酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％序列同一性的氨基酸序列。
考虑到例如期望的产物化合物的量、底物浓度对酶活性的影响、反应条件下酶的稳定性、和底物到产物的转化百分比，反应混合物中的底物化合物可以变化。在所述方法的一些实施方案中，合适的反应条件包括至少约0.5至约400g/L、1至约400g/L、5至约400g/L、约10至约400g/L、或约50至约400g/L的底物(II)、底物A1或底物A2的载量。在一些实施方案中，合适的反应条件包括至少约0.5g/L、至少约1g/L、至少约5g/L、至少约10g/L、至少约15g/L、至少约20g/L、至少约30g/L、至少约50g/L、至少约75g/L、至少约100g/L、至少约150g/L、至少约200g/L、至少约250g/L、至少约300g/L、至少约350g/L、至少约400g/L或甚至更大的底物(II)、底物A1或底物A2的载量。虽然本文提供的底物载量的值是基于化合物(II)、A1或A2的分子量，但是还预期，也可在方法中使用相等的摩尔量的化合物(II)、A1或A2的多种水合物和盐。
所述方法的合适的反应条件通常还包括在反应混合物中存在辅因子。因为工程化脱羧酶通常利用维生素B6家族的成员，所以反应条件可包括选自吡哆醛-5’-磷酸(也被称为磷酸吡哆醛、PLP、P5P)、吡哆辛(PN)、吡哆醛(PL)、吡多胺(PM)、和它们的磷酸化对应物磷酸吡哆醇(PNP)、和磷酸吡多胺(PMP)的辅因子。在一些实施方案中，合适的反应条件可包括选自PLP、PN、PL、PM、PNP和PMP的辅因子以约0.01g/L至约10g/L、约0.02g/L至约5g/L、约0.05g/L至约2.5g/L的浓度存在。在一些实施方案中，辅因子是PLP。相应地，在一些实施方案中，合适的反应条件可包括辅因子PLP以约0.01g/L至约10g/L、约0.02g/L至约5g/L、约0.05g/L至约2.5g/L的浓度存在。在一些实施方案中，反应条件包括约10g/L或更少、约5g/L或更少、约1.0g/L或更少、约0.1g/L或更少、约0.05g/L或更少、或约0.02g/L或更少的PLP浓度。
在反应的一些实施方案中(例如，其中使用完整细胞或裂解物)，辅因子天然地存在于细胞提取物且不需要补充。在反应的一些实施方案中(例如，利用部分地纯化的、或纯化的脱羧酶)，所述方法还可包括将辅因子添加至酶反应混合物的步骤。在一些实施方案中，辅因子在反应开始时添加和/或另外的辅因子在反应期间被添加。
在反应的实施方案中，反应条件可包括合适的pH。如以上所述，期望的pH或期望的pH范围可通过使用酸或碱、合适的缓冲剂、或缓冲和添加酸或碱的组合来保持。反应混合物的pH可在反应过程之前和/或期间控制。在一些实施方案中，合适的反应条件包括约3.0至约8.0的溶液pH、约4.0至约6.0的pH。在一些实施方案中，反应条件包括约3.0、3.5、4.0、4.5、5.0、5.5、6.0、6.5、7.0、7.5或8.0的溶液pH。
在本文的方法的实施方案中，例如考虑到在较高的温度下在反应速率上的增加、对于足够久的反应持续时间的酶的活性，合适的温度可被用于反应条件。相应地，在一些实施方案中，合适的反应条件包括约10℃至约60℃、约25℃至约50℃、约25℃至约40℃、或约25℃至约30℃的温度。在一些实施方案中，合适的反应温度包括约25℃、30℃、35℃、40℃、45℃、50℃、55℃或60℃的温度。在一些实施方案中，酶促反应期间的温度可以贯穿反应过程保持在一定温度。在一些实施方案中，酶促反应期间的温度可以在反应过程期间调整为温度曲线。
使用工程化脱羧酶的方法通常在水或溶剂中进行。合适的溶剂包括水性缓冲溶液、有机溶剂和/或共溶剂***，共溶剂***通常包括水性溶剂和有机溶剂。水性溶液(水或水性共溶剂***)可为pH-缓冲的或非缓冲的。在一些实施方案中，使用工程化脱羧酶多肽的方法通常地于包含以下的水性共溶剂***中进行：有机溶剂(例如，甲醇、乙醇、丙醇、异丙醇(IPA))、二甲基亚砜(DMSO)、二甲基甲酰胺(DMF)、乙酸异丙酯、乙酸乙酯、乙酸丁酯、1-辛醇、庚烷、辛烷、甲基叔-丁基醚(MTBE)、甲苯等)、离子液体(例如，四氟硼酸1-乙基4-甲基咪唑、四氟硼酸1-丁基-3-甲基咪唑、六氟磷酸1-丁基-3-甲基咪唑等)。水性共溶剂***中的有机溶剂组分可与水性组分混溶，提供单一的液相，或可与水性组分部分混溶或不混溶，提供双液相。脱羧反应过程中产生的二氧化碳有可能造成泡沫的形成，可以适当添加消泡剂。示例性的水性共溶剂***包含水和一种或多种有机溶剂。通常，选择水性共溶剂***的有机溶剂组分以便其不会完全使脱羧酶失活。通过用候选溶剂***中的感兴趣的确定的底物并利用诸如本文描述的酶活性测定而测量特定的工程化的脱羧酶的酶活性，合适的共溶剂***可被容易地鉴定。在方法的一些实施方案中，合适的反应条件包括水性共溶剂，所述水性共溶剂包含约1％至约100％(v/v)、约1％至约60％(v/v)、约2％至约60％(v/v)、约5％至约60％(v/v)、约10％至约60％(v/v)、约10％至约50％(v/v)或约10％至约40％(v/v)的浓度的乙醇。在方法的一些实施方案中，合适的反应条件包括含有至少约1％、5％、10％、15％、20％、25％、30％、35％、40％、45％、50％、55％或60％(v/v)的浓度的乙醇的水性共溶剂。
合适的反应条件可包括提供将底物化合物生物催化转化成其相应的产物化合物的反应参数的组合。相应地，在方法的一些实施方案中，反应参数的组合包括：(a)约10g/L至200g/L底物A1或底物A2的载量；(b)约0.5g/L至10g/L的工程化多肽浓度；(c)约3.0至8.0的pH；和(d)约10℃至60℃的温度。
示例性反应条件包括在表2以及实施例7或实施例8中提供的测定条件。
在进行本文描述的脱羧反应时，工程化的脱羧酶多肽可以部分地纯化的或纯化的酶、用编码酶的基因转化的完整细胞、和/或此类细胞的细胞提取物和/或裂解物的形式加入至反应混合物中。用编码工程化的脱羧酶的基因转化的完整细胞，或其细胞提取物、其裂解物，以及分离的酶可以多种不同的形式使用，包括固体(例如，冻干的、喷雾干燥的等)或半固体(例如，湿菌体等粗糊状物)。细胞提取物或细胞裂解物可通过沉淀(例如，硫酸铵、聚乙烯亚胺、热处理或类似处理)部分纯化，之后在冻干前进行除盐程序(例如，超滤、透析及类似程序)。任何酶制品可通过使用已知的交联剂诸如，例如戊二醛交联或固定到固相材料(例如树脂)而被稳定化。
在本文描述的脱羧反应的一些实施方案中，反应在本文描述的合适的反应条件下进行，其中工程化脱羧酶多肽被固定至固体支持物上。可用于固定进行脱羧反应的工程化脱羧酶的固体支持物包括但不限于微球或树脂，所述微球或树脂包含具有环氧官能团的聚甲基丙烯酸酯、具有氨基环氧官能团的聚甲基丙烯酸酯、具有十八烷基官能团的苯乙烯/DVB共聚物或具有十八烷基官能团的聚甲基丙烯酸酯。示例性固体支持物包括但不限于壳聚糖珠、EupergitC和SEPABEAD(Mitsubishi)，包括以下不同类型的SEPABEAD：EC-EP、EC-HFA/S、EXA252、EXE119和EXE120。
在一些实施方案中，其中工程化多肽可以分泌多肽的形式被表达，含有该分泌多肽的培养基可被用于本文的方法中。
在一些实施方案中，固体反应物(例如，酶、盐等)可以各种不同的形式提供给反应，包括粉末(例如，冻干的、喷雾干燥的等)、溶液、乳液、悬浮液等。反应物可使用为本领域普通技术人员共知的方法和仪器容易地冻干或喷雾干燥。例如，蛋白质溶液可以小量冷冻于-80℃，然后加入至预冷却的冻干室内，之后应用真空。
在一些实施方案中，加入反应物的顺序并不关键。反应物可同时一起加入至溶剂中(例如，单相溶剂、双相水性共溶剂体系等)，或者可选择地，一些反应物可分别地加入，以及一些在不同的时间点一起加入。例如，脱羧酶和脱羧酶底物可首先加入溶剂中。然后可加入并混合有机相。可选择地，脱羧酶底物可在添加至水相之前在有机相中预混合。
附图说明
图1.脱羧酶催化L-酪氨酸脱羧生成酪胺
图2.脱羧酶催化左旋多巴脱羧生成多巴胺
具体实施方式
下面用实施例来进一步说明本发明，但本发明并不受其限制。下列实施例中未注明具体条件的实验方法，通常按照常规条件，或按照制造厂商所建议的条件。
实施例1：基因克隆和表达载体的构建
源自于嗜热链球菌(Streptococcus thermophilus)的野生型的脱羧酶的氨基酸序列，可从NCBI上检索得到，然后通过本领域的常见技术将其对应的核酸合成出来并克隆到表达载体pACYC-Duet-1上。将重组表达质粒转化到E.coil BL21(DE3)的感受态细胞中，转化条件42℃，热击90秒，转化液涂布到含有氯霉素的LB平板上，37℃倒置培养过夜，即获得重组转化体。
实施例2：脱羧酶多肽的表达，和含有脱羧酶多肽的酶液的制备
本发明的湿菌体和酶液制作步骤如下：将实施例1所得的重组E.coli BL21，接种至装有50mL含氯霉素的LB培养基(蛋白胨10g/L，酵母浸出粉5g/L，氯化钠10g/L，pH值7.0±0.2,25℃)的250mL锥形瓶中，置于30℃，250rpm摇床振荡培养过夜。当培养液的OD 600达到2时，按5％(v/v)的接种量接入装有250mLTB培养基(胰蛋白胨12g/L，酵母浸出粉24g/L，磷酸氢二钠9.4g/L，磷酸氢二钾2.2g/L，pH值7.0±0.2,30℃)的1000mL锥形瓶中，置30℃，250rpm摇床振摇培养。当培养液的OD600达到0.6-0.8时，加入终浓度为1mM的IPTG作为诱导剂，30℃诱导20h后，将培养液离心(8000rpm，10分钟)；离心后弃上清液，收集细胞得到湿菌体。将所得的湿菌体悬浮于30mL的磷酸缓冲液中(PBS，pH7.0)，在冰浴中超声破碎，离心收集上清液，即得含有脱羧酶多肽的酶液。所得的酶液可直接用于反应，或经过预处理后用于反应。
按照摇瓶制备方式，同比例缩小至96孔板进行培养得到培养液，离心去除上清培养基得到湿菌体，可通过本领域所熟知的化学破碎方法得到酶液。
实施例3：酶液的预处理
将实施例2所得的酶液，置于30℃的水浴中，并搅拌使酶液受热均匀，48小时后，离心收集上清液，即得含预处理后的含有脱羧酶多肽的酶液。
实施例4：分析方法
左旋多巴和多巴胺的HPLC分析方法：分析柱为Ultimate-LP-C18反相硅胶柱，流动相为20mM醋酸铵：乙腈＝95:5，流速为1.5mL/min，检测波长为280nm，多巴胺的保留时间为1.97min，左旋多巴的保留时间为1.43min。
L-酪氨酸和酪胺的HPLC分析方法：分析柱为月旭-Ultimate-LP-C18反相硅胶柱，流动相为0.1％乙酸水溶液：乙腈＝50:50，流速为1.5mL/min，检测波长为280nm，酪胺的保留时间为0.7min，L-酪氨酸的保留时间为1.6min。
实施例5：脱羧酶突变文库的构建
这里所用到的都是商业试剂，较佳地选用Quikchange试剂盒(供应商：Agilent)。突变引物的序列设计按照试剂盒的说明进行。现以单个残基位置的饱和突变文库的构建为例说明。PCR体系为：5xBuffer 10uL，10mM dNTP 1uL，质粒DNA模板1uL(50ng/uL),上下游引物各取0.75uL(10uM)，高保真酶0.5uL,ddH2O 36uL。PCR引物在突变位置的密码子为NNK。
PCR扩增步骤为：(1)98℃，预变性3min；(2)98℃，变性10s；(3)72℃退火和延伸3min；步骤(2)～(3)重复25次；(5)72℃继续延伸10min，冷却至4℃。在PCR产物中加入2uLDpnI，37℃过夜酶切消除质粒模板。酶切后的PCR产物按转化E.coli BL21(DE3)感受态细胞，涂布到含氯霉素的LB平板上即得目标残基位置的饱和突变文库。
实施例6突变酶库的筛选
从琼脂平板挑取突变酶库的菌落，接种至含有氯霉素的LB培养基的96孔平底浅板中(每孔200uL LB培养基)置于摇床180rpm，80％的湿度、30℃过夜培养18～20h。当培养液的OD 600达到2～3时，从96孔平底浅板取20uL接种到含有氯霉素的TB培养基的96孔深孔板中(每孔400uLTB培养基)，置于摇床250rpm，30℃，湿度80％培养。当培养液的OD 600达到0.6～0.8时，加入终浓度为0.2mM的IPTG作为诱导剂，置于摇床250rpm，80％的湿度、30℃过夜诱导18～20h后，4000rpm离心10min去除菌液上清。加入200μL裂解缓冲液(100mM磷酸缓冲液，pH7.5，含1mg/mL溶菌酶)使菌体重悬浮，反复冻融使细胞破碎得到酶液；然后将酶液置于30℃、250rpm摇床震荡48小时以完成预处理。预处理完成后，将酶液在4000rpm下离心15分钟，每个孔中取100μL酶液上清到含反应液(底物50g/L，0.5M磷酸铵-磷酸缓冲液，0.2mM PLP，pH4.0～6.0)的96孔板中，30℃摇床振荡反应24小时后，将反应样品按实施例4的方法分析。
实施例7工程化脱羧酶多肽催化的生成多巴胺的反应工艺
在0.5M磷酸铵-磷酸缓冲液(pH5.5)中，加入含SEQ ID No：100多肽的酶液5％(v/v)或使最终蛋白浓度为0.5g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)，加入终浓度为100g/L的左旋多巴以及终浓度为0.2mM的磷酸吡哆醛，400rpm，30℃搅拌，反应期间鼓吹氮气，避免产物多巴胺被氧化。反应6小时后，左旋多巴脱羧生成多巴胺的转化率≥95％。
实施例8工程化脱羧酶多肽催化的生成酪胺的反应工艺
在0.5M磷酸铵-磷酸缓冲液(pH5.5)中，加入含SEQ ID No：80多肽的酶液5％(v/v)或使最终蛋白浓度为0.5g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)，加入终浓度为100g/L的L-酪氨酸以及终浓度为0.2mM的磷酸吡哆醛，400rpm，30℃搅拌。反应6小时后，L-酪氨酸脱羧生成酪胺的转化率≥95％。
实施例9脱羧酶多肽催化的生成多巴胺的反应工艺
在0.5M磷酸铵-磷酸缓冲液(pH5.5)中，加入含SEQ ID No：2多肽的酶液20％(v/v)或使最终蛋白浓度为2g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)，加入终浓度为100g/L的左旋多巴以及终浓度为0.2mM的磷酸吡哆醛，400rpm，30℃搅拌，反应期间鼓吹氮气，避免产物多巴胺被氧化。反应6小时后，左旋多巴脱羧生成多巴胺的转化率约为90％。
实施例10预处理后的脱羧酶多肽催化的生成多巴胺的反应
将新鲜制备的含SEQ ID No：2多肽的酶液(总蛋白浓度为10g/L)在30℃下预处理48小时后，在0.5M磷酸铵-磷酸缓冲液(pH5.5)中，加入该预处理后的酶液20％(v/v)，加入终浓度为100g/L的左旋多巴以及终浓度为0.2mM的磷酸吡哆醛，400rpm，30℃搅拌，反应期间鼓吹氮气，避免产物多巴胺被氧化。反应6小时后，左旋多巴脱羧生成多巴胺的转化率约为26％。
实施例11预处理后的工程化脱羧酶多肽催化的生成多巴胺的反应工艺
将新鲜制备的含SEQ ID No：100多肽的酶液(总蛋白浓度为10g/L)在30℃下预处理48小时后，在0.5M磷酸铵-磷酸缓冲液(pH5.5)中，加入该预处理后的酶液10％(v/v)，加入终浓度为100g/L的左旋多巴以及终浓度为0.2mM的磷酸吡哆醛，400rpm，30℃搅拌，反应期间鼓吹氮气，避免产物多巴胺被氧化。反应6小时后，左旋多巴脱羧生成多巴胺的转化率≥95％。
实施例12：发酵表达工程化脱羧酶多肽
将包含带有目标脱羧酶多肽表达质粒的大肠杆菌BL21(DE3)的单个微生物菌落接种到含30μg/mL氯霉素50mL LB肉汤(5.0g/L Yeast Extract、10g/L Tryptone、10g/L氯化钠)中。细胞在30℃摇床中250rpm摇动培养过夜(至少16小时)。当培养物的OD600是1.4至2.0时，从培养箱取出细胞，立即使用或储存在4℃。
将含有2.0L生长培养基的5L发酵罐在121℃高压蒸汽灭菌锅灭菌30分钟。向发酵罐接种过夜培养的大肠杆菌(生长在如上述的摇瓶中至初始OD600为1.4至2.0)。以夹套循环水控制温度保持在30℃。以200-800rpm搅动发酵罐，以2-8L/min向发酵容器供应空气以保持溶解氧水平为40％饱和或更大。通过加入25-28％v/v氢氧化铵保持培养物的pH在7.0。培养物的生长通过流加含500g/L食用葡萄糖右旋糖一水合物、12g/L氯化铵和5g/L硫酸镁七水合物的补料溶液来维持。培养物达到OD600为25±5后，将培养物温度继续维持在30℃，通过加入异丙基-β-D-硫代半乳糖苷(IPTG)至终浓度1mM来诱导脱羧酶的表达，发酵继续另外的18小时。使用Thermo MuLtifuge X3R离心机在4℃以8000rpm离心10分钟来收集湿菌体。
应理解，在阅读了本发明的上述内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。
序列表
<110> 宁波酶赛生物工程有限公司
<120> 脱羧酶多肽及其在制备酪胺和多巴胺中的应用
<130> JEP005
<160> 104
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
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agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
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gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 2
<211> 621
<212> PRT
<213> 嗜热链球菌(Streptococcus thermophilus)
<400> 2
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 3
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 3
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataatga tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 4
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 4
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asp Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 5
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 5
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccctg ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 6
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 6
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Leu Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 7
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 7
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gaagttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 8
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 8
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Glu Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 9
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 9
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctgga aaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 10
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 10
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Glu Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 11
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 11
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaaaaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 12
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 12
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Lys
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 13
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 13
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactgct gggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 14
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 14
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Leu Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 15
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 15
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcactg 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 16
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 16
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Leu Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 17
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 17
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacgat caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 18
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 18
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asp Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 19
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 19
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 20
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 20
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 21
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 21
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat actccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 22
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 22
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Thr Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 23
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 23
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaactatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 24
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 24
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Thr Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 25
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 25
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaagtgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 26
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 26
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Val Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 27
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 27
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct actggaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 28
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 28
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Trp Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 29
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 29
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccaggtgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgcg gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtagactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 30
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 30
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Val Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Arg Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 31
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 31
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttggtgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 32
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 32
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Gly Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 33
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 33
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tgtgatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 34
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 34
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Val Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 35
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 35
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat ccgaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 36
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 36
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Pro Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 37
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 37
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtgtcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat agtaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 38
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 38
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ser Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ser Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 39
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 39
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ctgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataactccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 40
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 40
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Ala Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ser Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 41
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 41
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat aaaccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cactgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 42
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 42
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Lys Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Thr Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 43
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 43
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtgttctggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 44
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 44
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Val Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 45
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 45
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactgct gggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 46
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 46
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Leu Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 47
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 47
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccctg ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacgac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 48
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 48
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Leu Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asp Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 49
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 49
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg cggtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct actggaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 50
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 50
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Ala Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Trp Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 51
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 51
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacgac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 52
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 52
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asp Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 53
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 53
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcaaagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg cggtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 54
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 54
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Ala Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 55
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 55
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccctg ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg cggtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct actggaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 56
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 56
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Leu Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Ala Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Trp Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 57
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 57
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac caccgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 58
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 58
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Thr Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 59
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 59
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggaa ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgtcgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgcgtgacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 60
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 60
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Glu Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Ser Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Arg Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 61
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 61
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggaa ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgcgtgacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 62
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 62
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Glu Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Arg Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 63
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 63
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggaa ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgtcgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 64
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 64
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Glu Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Ser Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 65
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 65
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc gtaactatat gccgcaggat ccgccgatga tcagctcgga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 66
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 66
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Arg Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ser Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 67
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 67
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc gtaactatat gccgcaggat ccgccgatga tcagcacgga acgtcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacatc gtaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 68
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 68
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Arg Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Thr Glu Arg Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Arg Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 69
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 69
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc gtaactatat gccgcaggat ccgccgatga tcagcgcgga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacatc gtaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 70
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 70
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Arg Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Arg Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 71
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 71
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaactatat gccgcaggat ccgccgatga tcagctcgga acagcagacc 180
agcccggact ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacatc gtaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 72
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 72
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ser Glu Gln Gln Thr Ser Pro Asp Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Arg Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 73
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 73
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgca agaactatat gccgcaggat ccgccgatga tcagcccgga acgtcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 74
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 74
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Lys Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Pro Glu Arg Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 75
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 75
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaactatat gccgcaggat ccgccgatga tcagcccgga acgtcagacc 180
agcccggact ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 76
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 76
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Pro Glu Arg Gln Thr Ser Pro Asp Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 77
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 77
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaactatat gccgcaggat ccgccgatga tcagcgcgga acgtcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacatc gtaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 78
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 78
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Arg Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Arg Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 79
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 79
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaactatat gccgcaggat ccgccgatga tcagcccgga acgtcagacc 180
agcccggact ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacatc gtaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 80
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 80
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Pro Glu Arg Gln Thr Ser Pro Asp Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Arg Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 81
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 81
atgagcaaca ccagtttcag cgcaaaagat acctatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 82
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 82
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Tyr Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 83
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 83
atgagcaaca ccagtttcag cgcaaaagat accactctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 84
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 84
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Thr Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 85
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 85
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtacgct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 86
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 86
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Thr Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 87
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 87
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggttttct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 88
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 88
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Phe Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 89
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 89
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgca ttaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 90
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 90
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Ile Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 91
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 91
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc tgaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 92
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 92
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Leu Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 93
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 93
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga aaggcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 94
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 94
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Arg Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 95
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 95
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga agttcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 96
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 96
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Val Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 97
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 97
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acatcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 98
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 98
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu His Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 99
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 99
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggctt taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtaaactggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 100
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 100
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Phe Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Lys Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 101
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 101
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtttgctggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 102
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 102
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Leu Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620
<210> 103
<211> 1863
<212> DNA
<213> 人工序列(artificial sequence)
<400> 103
atgagcaaca ccagtttcag cgcaaaagat accgatctga gtgcactgtt tatcggtgac 60
aaagcagaaa atggtcagct gtataaggac ctgctgggca aactggttga tgaacatctg 120
ggttggcgcc agaattatat gccgcaggat ccgccgatga tcagcgccga acagcagacc 180
agcccggaat ttcgtggtac cgtgaataat atgaaggacg ttctggatga gctgagcagt 240
cgcctgcgta gtcagagcgt tccgtggcat aatgccggtc gttattgggg ccacatgaat 300
agcgaaaccc tgatgccggc aattctggcc tataattatg caatgctgtg gaatggtaac 360
aacgtggcct atgaaagcag cccggcaacc agccagatgg aagaagaagt tggtctggaa 420
tttgccaccc tgatgggcta taccaatggt tggggccata ttgttgcaga tggtagcctg 480
gcaaatctgg aaggcctgtg gtatgcacgc aatattaaga gcctgccgtt tgccatgaaa 540
gccgttgatc cgaccattgt tgccggcaaa accgattggg aactgagtaa tatgagcacc 600
aaagaaatca tggacctggt tgaagccaat ggtgacaaaa ttgatgagat caaggccaaa 660
agcgcccgcg gcggtaaaga tctggataaa ctgggtaaat ggctggttcc gcagaccaaa 720
cattatagtt ggctgaaagc cgcagatatt atcggcattg gcctggatca ggttattccg 780
gttccggttg atagcaatta tcgtatggat atcaacgagc tggagaaaat tatccgcgaa 840
ctggcaagta ccgaaacccc gattctgggc gttgtgggcg ttgtgggtag taccgaagaa 900
ggtgccgttg atggcattaa tgaaattgca gaactgcgta acaagctggt gaaagaaggt 960
atctatttct acttccacat cgacgccgcc tatggtggct atggtcgcgc aattctgctg 1020
gatgaagata ataagctgat cccgtataag gaccttcaga gtaaattcgc cgaatataac 1080
gtgttcaccg aagaagaaaa cctggtgagt gaacatacct ataacgccta tgccgcattt 1140
ccggaagccg aaagtgttac cattgatccg cataaaatgg gctatatccc gtatagtgca 1200
ggcggcattg ccattcagga tattcgcatg cgtgatgtta tcagctattt cgcaacctat 1260
gtgttcgaaa agggcgcaga tattccggca ctgctgggtg cttatattct ggaaggcagt 1320
aaagcaggcg caaccgcagc cagtgtttgg gcagcacata aaaccctgcc gctgaatgtt 1380
accggttatg gtggtctggt tggtgcaagc attgaaggcg cccgccgttt ttataatttt 1440
ctgagcggcc tggaattcaa ggtgggcgat aaaaccattg aagttcatcc gctgaccgat 1500
ccggatttta atatggttga ttacgtgttc caggagaagg gcaataataa tctggtggaa 1560
atgaacgagc tgaaccatga attttacaac caggcaagct acgttaccgg tagcatctat 1620
ggcaatgaat atctgaccag ccataccgat ttcgcaattc cggattatgg taatagcccg 1680
tttaagtttg tgaacaacct gggcgtgacc gaagaaggct ggaaagaagc aggtaaagtg 1740
accgttctgc gcgccgctgt gatgacacct tatatgaata aggcagagaa cttcgactac 1800
ttcgccccga aaattaagca ggccatgcag gaaaaactgg aagccatcta tgaagatcgt 1860
aag 1863
<210> 104
<211> 621
<212> PRT
<213> 人工序列(artificial sequence)
<400> 104
Met Ser Asn Thr Ser Phe Ser Ala Lys Asp Thr Asp Leu Ser Ala Leu
1 5 10 15
Phe Ile Gly Asp Lys Ala Glu Asn Gly Gln Leu Tyr Lys Asp Leu Leu
20 25 30
Gly Lys Leu Val Asp Glu His Leu Gly Trp Arg Gln Asn Tyr Met Pro
35 40 45
Gln Asp Pro Pro Met Ile Ser Ala Glu Gln Gln Thr Ser Pro Glu Phe
50 55 60
Arg Gly Thr Val Asn Asn Met Lys Asp Val Leu Asp Glu Leu Ser Ser
65 70 75 80
Arg Leu Arg Ser Gln Ser Val Pro Trp His Asn Ala Gly Arg Tyr Trp
85 90 95
Gly His Met Asn Ser Glu Thr Leu Met Pro Ala Ile Leu Ala Tyr Asn
100 105 110
Tyr Ala Met Leu Trp Asn Gly Asn Asn Val Ala Tyr Glu Ser Ser Pro
115 120 125
Ala Thr Ser Gln Met Glu Glu Glu Val Gly Leu Glu Phe Ala Thr Leu
130 135 140
Met Gly Tyr Thr Asn Gly Trp Gly His Ile Val Ala Asp Gly Ser Leu
145 150 155 160
Ala Asn Leu Glu Gly Leu Trp Tyr Ala Arg Asn Ile Lys Ser Leu Pro
165 170 175
Phe Ala Met Lys Ala Val Asp Pro Thr Ile Val Ala Gly Lys Thr Asp
180 185 190
Trp Glu Leu Ser Asn Met Ser Thr Lys Glu Ile Met Asp Leu Val Glu
195 200 205
Ala Asn Gly Asp Lys Ile Asp Glu Ile Lys Ala Lys Ser Ala Arg Gly
210 215 220
Gly Lys Asp Leu Asp Lys Leu Gly Lys Trp Leu Val Pro Gln Thr Lys
225 230 235 240
His Tyr Ser Trp Leu Lys Ala Ala Asp Ile Ile Gly Ile Gly Leu Asp
245 250 255
Gln Val Ile Pro Val Pro Val Asp Ser Asn Tyr Arg Met Asp Ile Asn
260 265 270
Glu Leu Glu Lys Ile Ile Arg Glu Leu Ala Ser Thr Glu Thr Pro Ile
275 280 285
Leu Gly Val Val Gly Val Val Gly Ser Thr Glu Glu Gly Ala Val Asp
290 295 300
Gly Ile Asn Glu Ile Ala Glu Leu Arg Asn Lys Leu Val Lys Glu Gly
305 310 315 320
Ile Tyr Phe Tyr Phe His Ile Asp Ala Ala Tyr Gly Gly Tyr Gly Arg
325 330 335
Ala Ile Leu Leu Asp Glu Asp Asn Lys Leu Ile Pro Tyr Lys Asp Leu
340 345 350
Gln Ser Lys Phe Ala Glu Tyr Asn Val Phe Thr Glu Glu Glu Asn Leu
355 360 365
Val Ser Glu His Thr Tyr Asn Ala Tyr Ala Ala Phe Pro Glu Ala Glu
370 375 380
Ser Val Thr Ile Asp Pro His Lys Met Gly Tyr Ile Pro Tyr Ser Ala
385 390 395 400
Gly Gly Ile Ala Ile Gln Asp Ile Arg Met Arg Asp Val Ile Ser Tyr
405 410 415
Phe Ala Thr Tyr Val Phe Glu Lys Gly Ala Asp Ile Pro Ala Leu Leu
420 425 430
Gly Ala Tyr Ile Leu Glu Gly Ser Lys Ala Gly Ala Thr Ala Ala Ser
435 440 445
Val Trp Ala Ala His Lys Thr Leu Pro Leu Asn Val Thr Gly Tyr Gly
450 455 460
Gly Leu Val Gly Ala Ser Ile Glu Gly Ala Arg Arg Phe Tyr Asn Phe
465 470 475 480
Leu Ser Gly Leu Glu Phe Lys Val Gly Asp Lys Thr Ile Glu Val His
485 490 495
Pro Leu Thr Asp Pro Asp Phe Asn Met Val Asp Tyr Val Phe Gln Glu
500 505 510
Lys Gly Asn Asn Asn Leu Val Glu Met Asn Glu Leu Asn His Glu Phe
515 520 525
Tyr Asn Gln Ala Ser Tyr Val Thr Gly Ser Ile Tyr Gly Asn Glu Tyr
530 535 540
Leu Thr Ser His Thr Asp Phe Ala Ile Pro Asp Tyr Gly Asn Ser Pro
545 550 555 560
Phe Lys Phe Val Asn Asn Leu Gly Val Thr Glu Glu Gly Trp Lys Glu
565 570 575
Ala Gly Lys Val Thr Val Leu Arg Ala Ala Val Met Thr Pro Tyr Met
580 585 590
Asn Lys Ala Glu Asn Phe Asp Tyr Phe Ala Pro Lys Ile Lys Gln Ala
595 600 605
Met Gln Glu Lys Leu Glu Ala Ile Tyr Glu Asp Arg Lys
610 615 620

Claims (19)

1.一种工程化脱羧酶多肽，所述脱羧酶多肽的氨基酸序列与SEQ ID NO:2的序列相比存在残基差异X51P，所述多肽的氨基酸序列为SEQ ID No：20、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104所示的氨基酸序列。
2.一种通过化学键或物理吸附的方法被固定在固体材质上的多肽，所述多肽选自权利要求1所述的脱羧酶多肽。
3.一种多核苷酸，所述多核苷酸编码权利要求1的多肽。
4.如权利要求3所述的多核苷酸，其中所述多核苷酸序列为对应SEQ ID No：19、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103所示的多核苷酸序列。
5.一种表达载体，所述表达载体包含权利要求3-4任一项所述的多核苷酸。
6.如权利要求5所述的表达载体，所述表达载体包括质粒、粘粒、噬菌体或病毒载体。
7.一种宿主细胞，所述宿主细胞包含权利要求5的表达载体，所述宿主细胞为大肠杆菌。
8.一种脱羧酶多肽的制备方法，其包括如下步骤：培养权利要求7所述的宿主细胞，以及从培养物中获得脱羧酶多肽。
9.一种脱羧酶催化剂，其选自权利要求8的培养物，通过从权利要求8的培养物中获得的含脱羧酶多肽的宿主细胞或培养液，或者用其加工的制品；其中，所述制品是指由权利要求7或8所述的宿主细胞得到的提取物，通过对所述提取物中的脱羧酶进行分离或纯化得到的分离产品，或通过固定化权利要求7或8所述的宿主细胞及其提取物或提取物的分离产品而得到的固定化制品。
10.一种制备结构式(I)化合物的方法：
Figure FDA0003320650240000011
其中R是任选地取代的C1-C8烃基，或是任选地取代的芳基或杂芳基；所述方法包括，将结构式(II)的氨基酸底物：
Figure FDA0003320650240000012
与权利要求1的工程化多肽接触的步骤。
11.如权利要求10所述的方法，其中结构式(I)的化合物是
Figure FDA0003320650240000021
其中其中R1是-H、-CH2OH、-CH2SH、-CH2SCH3或任选地取代的C1-C4烃基；R2是C1-C4烃基、-H、卤素、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-CNR’、-CONR’、-SO2NH2或-SONH2、-CN、CF3，其中每个R’独立地选自-H或C1-C4烃基；R3是C1-C4烃基、-H、卤素、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-CNR’、-CONR’、-SO2NH2或-SONH2、-CN、CF3，其中每个R’独立地选自-H或C1-C4烃基；
且结构式(II)的氨基酸底物是：
Figure FDA0003320650240000022
12.如权利要求11所述的方法，其中R2在苯基环的对位，或者R2在苯基环的间位，或者R2在苯基环的邻位；或者R2同时在苯基环的对位和间位，或者R2同时在苯基环的对位和邻位，或者R2同时在苯基环的间位和邻位。
13.如权利要求10所述的方法，其中结构式(I)的化合物是：
Figure FDA0003320650240000023
14.一种制备酪胺的方法：
Figure FDA0003320650240000024
所述方法包括，将结构式A1化合物
Figure FDA0003320650240000025
与权利要求1的工程化脱羧酶多肽接触的步骤。
15.一种制备多巴胺的方法：
Figure FDA0003320650240000031
所述方法包括，将结构式A2化合物
Figure FDA0003320650240000032
与权利要求1的工程化脱羧酶多肽接触的步骤。
16.如权利要求10-15任一项所述的方法，反应溶剂包括水，甲醇，乙醇，丙醇，异丙醇，乙酸异丙酯，二甲基亚砜或二甲基甲酰胺。
17.如权利要求10-15任一项所述的方法，其中反应体系的温度为10℃至60℃。
18.如权利要求10-15任一项所述的方法，其中反应体系的pH为pH3.0至pH8.0。
19.如权利要求10-15任一项所述的方法，底物以5g/L至400g/L的载量存在。
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