CN110330565B - 从血浆分离组分ⅰ和ⅲ中提取静注人免疫球蛋白的方法 - Google Patents
从血浆分离组分ⅰ和ⅲ中提取静注人免疫球蛋白的方法 Download PDFInfo
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- CN110330565B CN110330565B CN201910625276.1A CN201910625276A CN110330565B CN 110330565 B CN110330565 B CN 110330565B CN 201910625276 A CN201910625276 A CN 201910625276A CN 110330565 B CN110330565 B CN 110330565B
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- BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 claims abstract description 24
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
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- Organic Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
标号 | 辛酸钠终浓度(mM) | 1M辛酸钠加入量(ml) | PH值 |
A1 | 20 | 4 | 5.5 |
B1 | 20 | 4 | 5.2 |
C1 | 20 | 4 | 4.9 |
D1 | 30 | 6 | 5.5 |
E1 | 30 | 6 | 5.2 |
F1 | 30 | 6 | 4.9 |
G1 | 40 | 8 | 5.5 |
H1 | 40 | 8 | 5.2 |
I1 | 40 | 8 | 4.9 |
J1 | 50 | 10 | 5.5 |
K1 | 50 | 10 | 5.2 |
L1 | 50 | 10 | 4.9 |
标号 | 辛酸钠终浓度(mM) | 1M辛酸钠加入量(ml) | PH值 |
A2 | 20 | 2 | 5.5 |
B2 | 20 | 2 | 5.2 |
C2 | 20 | 2 | 4.9 |
D2 | 30 | 3 | 5.5 |
E2 | 30 | 3 | 5.2 |
F2 | 30 | 3 | 4.9 |
G2 | 40 | 4 | 5.5 |
H2 | 40 | 4 | 5.2 |
I2 | 40 | 4 | 4.9 |
J2 | 50 | 5 | 5.5 |
K2 | 50 | 5 | 5.2 |
L2 | 50 | 5 | 4.9 |
步骤 | 柱体积(CV) | Buffer | 线性流速cm/h |
平衡1 | 2 | Buffer A | 150 |
平衡2 | 5 | Buffer B | 150 |
上样 | 67.89(13.578kg) | 前处理样品 | 100 |
清洗 | 2 | Buffer B | 100 |
CIP | 1.5 | Buffer C | 30 |
步骤 | 柱体积(CV) | Buffer | 线性流速cm/h |
平衡1 | 2 | Buffer D | 150 |
平衡2 | 5 | Buffer E | 150 |
上样 | 51.21(15.364kg) | 预处理样品 | 75 |
清洗 | 2 | Buffer E | 75 |
CIP | 1.5 | Buffer C | 30 |
Claims (5)
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CN201910625276.1A CN110330565B (zh) | 2019-07-11 | 2019-07-11 | 从血浆分离组分ⅰ和ⅲ中提取静注人免疫球蛋白的方法 |
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CN111454353A (zh) * | 2020-04-03 | 2020-07-28 | 山东泰邦生物制品有限公司 | 一种从血浆组分fi+iii沉淀中制备静注人免疫球蛋白的方法 |
BE1029863B1 (nl) * | 2022-05-10 | 2023-05-12 | Prothya Biosolutions Belgium | WERKWIJZEN VOOR HET PRODUCEREN VAN IMMUNOGLOBULINE G (IgG) PREPARATEN EN/OF OPLOSSINGEN |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102250240A (zh) * | 2011-06-27 | 2011-11-23 | 山东泰邦生物制品有限公司 | 一种从血浆分离组分ⅰ+ⅲ中纯化人免疫球蛋白的方法 |
CN102532307A (zh) * | 2012-02-22 | 2012-07-04 | 成都蓉生药业有限责任公司 | 一种制备人免疫球蛋白的方法 |
CN102584934A (zh) * | 2012-03-19 | 2012-07-18 | 江西博雅生物制药股份有限公司 | 一种静注人免疫球蛋白的制备工艺 |
CN109627329A (zh) * | 2018-12-27 | 2019-04-16 | 成都蓉生药业有限责任公司 | 一种人免疫球蛋白制备时去除和灭活病毒的方法 |
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US6955917B2 (en) * | 1997-06-20 | 2005-10-18 | Bayer Healthcare Llc | Chromatographic method for high yield purification and viral inactivation of antibodies |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102250240A (zh) * | 2011-06-27 | 2011-11-23 | 山东泰邦生物制品有限公司 | 一种从血浆分离组分ⅰ+ⅲ中纯化人免疫球蛋白的方法 |
CN102532307A (zh) * | 2012-02-22 | 2012-07-04 | 成都蓉生药业有限责任公司 | 一种制备人免疫球蛋白的方法 |
CN102584934A (zh) * | 2012-03-19 | 2012-07-18 | 江西博雅生物制药股份有限公司 | 一种静注人免疫球蛋白的制备工艺 |
CN109627329A (zh) * | 2018-12-27 | 2019-04-16 | 成都蓉生药业有限责任公司 | 一种人免疫球蛋白制备时去除和灭活病毒的方法 |
Non-Patent Citations (1)
Title |
---|
从血浆分离的组分I+III中纯化免疫球蛋白G;廉婷婷;《中国优秀硕士学位沦为全文数据库(电子期刊)》;20140315;全文 * |
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Inventor after: Wang Yue Inventor after: Yu Jianhong Inventor after: Liu Ying Inventor after: Zhang Yun Inventor after: Zheng Xiaobei Inventor after: Yang Dandan Inventor after: Gong Qin Inventor after: Liu Qi Inventor after: Luo Hong Inventor after: Zuo Lin Inventor after: Han Ren Inventor after: Li Cesheng Inventor after: Peng Liangjun Inventor after: Fang Bin Inventor after: Dong Tianbao Inventor after: Mei Feng Inventor after: Hu Yong Inventor after: Yue Shenglan Inventor before: Wang Yue Inventor before: Dong Tianbao Inventor before: Gong Qin Inventor before: Yue Shenglan Inventor before: Zheng Xiaobei Inventor before: Luo Hong Inventor before: Zhang Yun Inventor before: Yang Dandan Inventor before: Zuo Lin Inventor before: Mei Feng Inventor before: Fang Bin Inventor before: Peng Liangjun Inventor before: Han Ren Inventor before: Yu Jianhong Inventor before: Li Cesheng Inventor before: Liu Ying Inventor before: Hu Yong Inventor before: Liu Qi |
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Address after: 430207 No.1-1, Huangjin Industrial Park Road, Zhengdian, Jiangxia District, Wuhan City, Hubei Province Patentee after: Wuhan Biopharmaceutical Co., Ltd. of China National Pharmaceutical Group Address before: 430207 No.1-1, Huangjin Industrial Park Road, Zhengdian, Jiangxia District, Wuhan City, Hubei Province Patentee before: SINOPHARM WUHAN BLOOD PRODUCT CO.,LTD. |
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