CN110327394A - The extracting method of blood sugar reducing component in clausenae Lansium or its pericarp - Google Patents
The extracting method of blood sugar reducing component in clausenae Lansium or its pericarp Download PDFInfo
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- CN110327394A CN110327394A CN201910757465.4A CN201910757465A CN110327394A CN 110327394 A CN110327394 A CN 110327394A CN 201910757465 A CN201910757465 A CN 201910757465A CN 110327394 A CN110327394 A CN 110327394A
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- calusena lansium
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- 238000000034 method Methods 0.000 title claims abstract description 21
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- 239000000284 extract Substances 0.000 claims abstract description 79
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- 238000001914 filtration Methods 0.000 claims abstract description 17
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- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- OETHQSJEHLVLGH-UHFFFAOYSA-N metformin hydrochloride Chemical compound Cl.CN(C)C(=N)N=C(N)N OETHQSJEHLVLGH-UHFFFAOYSA-N 0.000 description 2
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- PQMOXTJVIYEOQL-UHFFFAOYSA-N Cumarin Natural products CC(C)=CCC1=C(O)C(C(=O)C(C)CC)=C(O)C2=C1OC(=O)C=C2CCC PQMOXTJVIYEOQL-UHFFFAOYSA-N 0.000 description 1
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- 229940122355 Insulin sensitizer Drugs 0.000 description 1
- FSOGIJPGPZWNGO-UHFFFAOYSA-N Meomammein Natural products CCC(C)C(=O)C1=C(O)C(CC=C(C)C)=C(O)C2=C1OC(=O)C=C2CCC FSOGIJPGPZWNGO-UHFFFAOYSA-N 0.000 description 1
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- 208000030159 metabolic disease Diseases 0.000 description 1
- 229960004329 metformin hydrochloride Drugs 0.000 description 1
- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin hydrochloride Natural products CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
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- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Diabetes (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Botany (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Obesity (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to biological fields, in particular to the extracting method of blood sugar reducing component in clausenae Lansium or its pericarp.After the present invention is by taking clausenae Lansium or its pericarp to crush, after water extract-alcohol precipitation or alcohol extracting, filtering, collect extracting solution, it is mentioned using water or alcohol extracting extracts the blood sugar reducing component in clausenae Lansium or its pericarp, with the characteristic that more excellent hypoglycemic ability and safety are mild, blood glucose can be effectively reduced.And pericarp pericarp is turned waste into wealth, it has important practical significance.
Description
Technical field
The present invention relates to biological fields, in particular to the extracting method of blood sugar reducing component in clausenae Lansium or its pericarp.
Background technique
Diabetes are one group of metabolic diseases characterized by hyperglycemia.Hyperglycemia be then due to defect of insulin secretion or
Its biological effect is impaired, or both have concurrently and cause.Long-standing hyperglycemia when diabetes, cause various tissues, especially eye,
Kidney, heart, blood vessel, the chronic lesion of nerve, dysfunction.Investigation display, global diabetes adult patient (20-79 years old) from
1.51 hundred million in 2000 had reached 4.25 hundred million by 2017, increased nearly 2 times.It expects 2045, diabetic is likely to be breached
6.29 hundred million.Diabetes have become one of the ten big diseases for seriously endangering our health.
That treats diabetes at present relies primarily on drug therapy, and common drug includes sulfonylurea drugs, biguanides hypoglycemic
Medicine, alpha-glucosidase restrainer, insulin sensitizer, meglitinide Insulin secretagogues and insulin therapy.Drug tool
There are many side reactions and adverse reaction, such as Nausea and vomiting, loss of appetite, abdominal pain, diarrhea etc., and takes or inject
Inconvenience brings many pains and puzzlement to patient, there is an urgent need to further develop safely, effectively, convenient hypoglycemic product.
For Calusena lansium as one of southern characteristic fruit, Calusena lansium also has very high medical value, the fruit of Calusena lansium, leaf, root, seed
It can be used as medicine, and be dual-purpose of drug and food.The fruit of Calusena lansium has promoting the circulation of qi, helps digestion, clears phlegm, cures mainly dyspepsia turgor, epigastric pain,
Colic, phlegm retention, cough and asthma.Amides compound has liver protection, anti-senile dementia disease and lipoid peroxidization resistant in Calusena lansium, but not
See the relevant report of its hypoglycemic, and Calusena lansium Time To Market is limited, it is relatively difficult in terms of fresh preservation, thus seldom seen in the north
Calusena lansium fresh fruit, application surface and time are not wide enough.And in order to reach drug effect, a certain amount of Calusena lansium is needed, can also be brought not to patient
Just.Therefore, extracting the effective component in Calusena lansium, and the product for being conveniently easy to carry about with one and eating is made, have important
Realistic meaning.
Summary of the invention
In view of this, the present invention provides a kind of extracting method of blood sugar reducing component in clausenae Lansium or its pericarp.The present invention is logical
It crosses that water mentions or alcohol extracting extracts the blood sugar reducing component in Calusena lansium, is prepared into a kind of novel product, has more excellent
Hypoglycemic ability and the mild characteristic of safety, can be effectively reduced hyperglycemia.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides the extracting methods of blood sugar reducing component in Calusena lansium, after taking Calusena lansium to crush, through water extract-alcohol precipitation or alcohol extracting
Afterwards, extracting solution, filtering are collected；
The water extract-alcohol precipitation specifically: take smashed Calusena lansium, add water heating and refluxing extraction, collect Aqueous extracts, be concentrated into
Solid-liquid ratio 1:1 is slow added into ethyl alcohol and carries out alcohol precipitation, collects supernatant liquid filtering, obtains precipitation solution, concentration；
The alcohol extracting specifically: take smashed Calusena lansium to mix heated refluxing extraction with ethyl alcohol, collect alcohol extract, mistake
Filter, concentration；
The water extracts or ethyl alcohol extraction stands alone as heating and refluxing extraction or ultrasonic extraction；
The temperature of the heating and refluxing extraction is 70 DEG C~100 DEG C, and the time is 1.5h~3h, and number is 3~4 times；
The frequency of the ultrasonic extraction is 60W/L, and the time is 1h~3h, and number is 4~6 times.
In some specific embodiments of the invention, in the water extract-alcohol precipitation, smashed Calusena lansium and water quality
Than for 1:(5~15)；The volume ratio of the Aqueous extracts and ethyl alcohol is 1:1；
In the alcohol extracting smashed Calusena lansium and ethyl alcohol mass ratio be 1:(5~10)；
The concentration of ethyl alcohol is 75%~95% in the alcohol extracting；
The water extract-alcohol precipitation concentration is 50%~70%.
In some specific embodiments of the invention, concentration is further included the steps that after the filtering；The thickening temperature
It is 35 DEG C~50 DEG C, vacuum degree 50hPa~200hPa is concentrated into solid-liquid ratio 1:1~1:1.3.
In some specific embodiments of the invention, freeze-drying, the vacuum are further included the steps that after the concentration
The temperature of freeze-drying is -50 DEG C -10 DEG C, and vacuum degree is 1.3Pa~13Pa.
The present invention also provides extracts made from the extracting method.
On the basis of the studies above, the present invention also provides the extracts to prepare hypoglycemic drug, food
Or the application in drink.
In addition, the present invention also provides drink, including the extract and common auxiliary material.
In some specific embodiments of the invention, the auxiliary material includes maltodextrin, D-sorbite and mangosteen powder；
In terms of mass parts, the drink has following component to be made:
The present invention also provides drugs, including the extract and pharmaceutically acceptable auxiliary material.
The present invention also provides food, including acceptable auxiliary material in the extract and food.
Blood sugar reducing component in clausenae Lansium of the present invention or its pericarp includes Coumarins and saponin component.The present invention
It is mentioned by water or alcohol extracting extracts the blood sugar reducing component in clausenae Lansium or its pericarp, be prepared into a kind of novel product,
With the characteristic that more excellent hypoglycemic ability and safety are mild, hyperglycemia can be effectively reduced.And pericarp is turned waste into wealth,
It has important practical significance.
Specific embodiment
The invention discloses a kind of extracting methods of blood sugar reducing component in clausenae Lansium or its pericarp, and those skilled in the art can
To use for reference present disclosure, it is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to this
It is it will be apparent that they are considered as being included in the present invention for the technical staff of field.Method and application of the invention is
Be described by preferred embodiment, related personnel obviously can not depart from the content of present invention, in spirit and scope to herein
The methods and applications are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
The purpose of the present invention is to provide the blood sugar reducing components of Calusena lansium, so that the extract has higher hypoglycemic energy
Power.
It is a further object to provide the extracting methods of the Calusena lansium blood sugar reducing component and the Calusena lansium to drop blood
Related application in sugar product.
To achieve the goals above, the invention provides the following technical scheme:
Blood sugar reducing component in Calusena lansium, water extract or alcohol extract including Calusena lansium.
In selected extract, cumarin has good antitumor, antibacterial, antimycotic, blood coagulation resisting function, hypoglycemic and pre-
The effect of preventing bone rarefaction；Saponin component have antibacterial, antipyretic, calm, anticancer, it is hypoglycemic, keep fit and healthy and other effects.This hair
It is bright according to ingredients these two types of in Calusena lansium to there is hypoglycemic ability to treat hyperglycemia.
Preferably, the water extract and alcohol extract is heated boils extraction or heating and refluxing extraction or ultrasonic extraction obtains
?.
Meanwhile the present invention also provides the extracting method of blood sugar reducing component in the Calusena lansium, water is added after Calusena lansium is smashed to pieces
Or extraction or heating and refluxing extraction or ultrasonic extraction are boiled in ethyl alcohol heating, concentration is medicinal extract after ethanol extract filtering, and water extracts
Object filters after water extract-alcohol precipitation to be concentrated again as medicinal extract, and the Chinese medicine composition is obtained.
Wherein, preferably, the amount of water or amount of alcohol is added to be 5-15 times of four kinds of medicinal material gross weights；The concentration of ethyl alcohol is not
Limit, can be preferably 75% concentration and 95% concentration.
Preferably, extraction is boiled in the heating or heating and refluxing extraction is to extract 3 times, the ultrasonic extraction is 4 times；
Preferably, the extraction time is every time 30~90min.
Blood sugar reducing component is big to external alpha-glucosaccharase enzyme inhibition activity and to diabetes B in Calusena lansium of the present invention
In the influence test of mouse, there is better hypoglycemic ability compared to street drug acarbose.Also, in vitro in antibacterial tests
Also superior to each processing group.Meanwhile being tropical fruit (tree) to Calusena lansium of the present invention, it is convenient that solid beverage is made in non-toxic and safe.
Based on above-mentioned technical effect, the present invention provides blood sugar reducing components in the Calusena lansium to treat hyperglycemia product in preparation
In application.
Preferably, the product is solid beverage.
In addition, the present invention also provides a kind of solid beverage product, including mentioning for Calusena lansium blood sugar reducing component of the present invention
Take object and auxiliary material.
Preferably, the auxiliary material is maltodextrin, D-sorbite and mangosteen powder.
It is highly preferred that dosage of each component is as follows in solid beverage:
Calusena lansium extract 50~70%, maltodextrin 5~20%, D-sorbite 10~40%, mangosteen powder 1~10%.
In some embodiments of the invention, dosage of each component are as follows:
Calusena lansium extract 50%, maltodextrin 5%, D-sorbite 40%, mangosteen powder 5%.
Calusena lansium extract 60%, maltodextrin 20%, D-sorbite 10%, mangosteen powder 10%.
Calusena lansium extract 70%, maltodextrin 10%, D-sorbite 19%, mangosteen powder 1%.
From the above technical scheme, the present invention selects Calusena lansium to prepare a kind of extract and the medicine containing the extract
Object, food or drink have good therapeutic effect to hyperglycemia, safe and convenient.
In the present invention, the clausenae Lansium includes skin, fruit and seed.
Raw materials used and reagent in the extracting method of blood sugar reducing component in clausenae Lansium provided by the invention or its pericarp
It is bought by market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1: the extraction of blood sugar reducing component in Calusena lansium of the present invention
After taking Calusena lansium to smash to pieces be added 5 times water in 100 DEG C heating and refluxing extraction 3 times, extract 1.5h, total 4.5h every time.It closes
And extracting solution filters, and takes filtrate, solid-liquid ratio is concentrated into 50 DEG C, and (heretofore described " solid-liquid ratio " is the quality of solid " material "
With the ratio of the volume of " liquid " as leaching liquor) it is 1:1, the concentration for adding same volume is 55% (v/v) ethyl alcohol, carries out alcohol
It is heavy, impurities in water is removed, takes ethanol extract to filter, the medicinal extract of no ethyl alcohol is concentrated into when 45 DEG C, through vacuum freeze drying
Temperature is -10 DEG C, and vacuum degree 13Pa obtains the Chinese medicine composition.
Embodiment 2: the extraction of blood sugar reducing component in Calusena lansium of the present invention
After taking Calusena lansium to smash to pieces be added 10 times water in 70 DEG C heating and refluxing extraction 4 times, extract 3h, total 12h every time.Merging mentions
It takes liquid to filter, takes filtrate, solid-liquid ratio is concentrated into 35 DEG C, and (heretofore described " solid-liquid ratio " is the quality and work of solid " material "
For the ratio of the volume of " liquid " of leaching liquor) it is 1:1.2, the concentration for adding same volume is 60% (v/v) ethyl alcohol, alcohol precipitation is carried out,
Impurities in water is removed, takes ethanol extract to filter, the medicinal extract of no ethyl alcohol, the temperature through vacuum freeze drying is concentrated into when 40 DEG C
It is -50 DEG C, vacuum degree 1.3Pa obtains the Chinese medicine composition.
Embodiment 3: the extraction of blood sugar reducing component in Calusena lansium of the present invention
After taking Calusena lansium to smash to pieces be added 15 times water in 100 DEG C heating and refluxing extraction 3 times, extract 3h, total 9h every time.Merging mentions
It takes liquid to filter, takes filtrate, solid-liquid ratio is concentrated into 40 DEG C, and (heretofore described " solid-liquid ratio " is the quality and work of solid " material "
For the ratio of the volume of " liquid " of leaching liquor) it is 1:1.3, the concentration for adding same volume is 65% (v/v) ethyl alcohol, alcohol precipitation is carried out,
Impurities in water is removed, takes ethanol extract to filter, the medicinal extract of no ethyl alcohol, the temperature through vacuum freeze drying is concentrated into when 8 DEG C
It is -30 DEG C, vacuum degree 3Pa obtains the Chinese medicine composition.
Embodiment 4: the extraction of blood sugar reducing component in Calusena lansium of the present invention
The water that 8 times are added after taking Calusena lansium to smash to pieces extracts 3h, total 12h in 60W/L ultrasonic extraction 4 times every time.Combined extract
Filtering, takes filtrate, alcohol extract is taken to filter, and is 40 DEG C, vacuum degree 100hPa in temperature, being concentrated into solid-liquid ratio is 1:1.3, through vacuum
The temperature of freeze-drying is -10 DEG C, and vacuum degree 1.3Pa obtains the Chinese medicine composition.
Embodiment 5: the extraction of blood sugar reducing component in Calusena lansium of the present invention
The water that 10 times are added after taking Calusena lansium to smash to pieces extracts 1h, total 6h in 60W/L ultrasonic extraction 6 times every time.Combined extract
Filtering, takes filtrate, alcohol extract is taken to filter, and is 45 DEG C, vacuum degree 110hPa in temperature, being concentrated into solid-liquid ratio is 1:1, cold through vacuum
It is -20 DEG C that dry temperature, which is lyophilized, and vacuum degree 3Pa obtains the Chinese medicine composition.
Embodiment 6: the extraction of blood sugar reducing component in Calusena lansium of the present invention
The water that 12 times are added after taking Calusena lansium to smash to pieces extracts 1.5h, total 7.5h in 60W/L ultrasonic extraction 5 times every time.Merging mentions
It takes liquid to filter, takes filtrate, alcohol extract is taken to filter, be 50 DEG C, vacuum degree 120hPa in temperature, being concentrated into solid-liquid ratio is 1:1.1, warp
The temperature of vacuum freeze drying is -30 DEG C, and vacuum degree 5Pa obtains the Chinese medicine composition.
Embodiment 7: the extraction of blood sugar reducing component in Calusena lansium of the present invention
After taking Calusena lansium to smash to pieces be added 8 times 75% ethyl alcohol in 85 DEG C heating and refluxing extraction 3 times, extract 90min every time.Merge
Extracting solution filtering, takes filtrate, is 35 DEG C in temperature, solvent-free medicinal extract is concentrated under the conditions of vacuum degree 80hPa, through vacuum refrigeration
Dry temperature is -50 DEG C, and vacuum degree 8Pa obtains the Chinese medicine composition.
Embodiment 8: the extraction of blood sugar reducing component in Calusena lansium of the present invention
After taking Calusena lansium to smash to pieces be added 10 times 85% ethyl alcohol in 80 DEG C heating and refluxing extraction 3 times, extract 1.5h every time.Merge
Extracting solution filtering, takes filtrate, is 40 DEG C in temperature, the medicinal extract of no ethyl alcohol is concentrated under the conditions of vacuum degree 90hPa, through vacuum refrigeration
Dry temperature is -20 DEG C, and vacuum degree 13Pa obtains the Chinese medicine composition.
Embodiment 9: the extraction of blood sugar reducing component in Calusena lansium of the present invention
12 times of 95% ethyl alcohol is added after taking Calusena lansium to smash to pieces and boils extraction 4 times in 75 DEG C of heating, extracts 1.5h every time, altogether
6h.Combined extract filtering, takes filtrate, is 45 DEG C in temperature, the medicinal extract of no ethyl alcohol, warp are concentrated under the conditions of vacuum degree 100hPa
The temperature of vacuum freeze drying is -10 DEG C, and vacuum degree 15Pa obtains the Chinese medicine composition.
Embodiment 10: the extraction of blood sugar reducing component in Calusena lansium of the present invention
95% ethyl alcohol that 10 times are added after taking Calusena lansium to smash to pieces mentions every time ultrasonic extraction 6 times under conditions of frequency is 60W/L
Take 30min, total 3h.Combined extract filtering, takes filtrate, is 45 DEG C in temperature, is concentrated into no ethyl alcohol under the conditions of vacuum degree 10Pa
Medicinal extract, through -50 DEG C be freeze-dried, obtain the Chinese medicine composition.
Embodiment 11: the extraction of blood sugar reducing component in Calusena lansium of the present invention
90% ethyl alcohol that 15 times are added after taking Calusena lansium to smash to pieces mentions every time ultrasonic extraction 4 times under conditions of frequency is 60W/L
Take 30min, total 2h.Combined extract filtering, takes filtrate, is 40 DEG C in temperature, is concentrated into no second under the conditions of vacuum degree 100hPa
The medicinal extract of alcohol, the temperature through vacuum freeze drying are -20 DEG C, and vacuum degree 1.3Pa obtains the Chinese medicine composition.
Embodiment 12: the extraction of blood sugar reducing component in Calusena lansium of the present invention
85% ethyl alcohol that 20 times are added after taking Calusena lansium to smash to pieces mentions every time ultrasonic extraction 6 times under conditions of frequency is 60W/L
Take 30min, total 3h.Combined extract filtering, takes filtrate, is 35 DEG C in temperature, is concentrated into no ethyl alcohol under the conditions of vacuum degree 70hPa
Medicinal extract, the temperature through vacuum freeze drying be -30 DEG C, vacuum degree 13Pa obtains the Chinese medicine composition.
Embodiment 13: solid beverage of the present invention
1 Calusena lansium extract 50% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 10%.
Embodiment 14: solid beverage of the present invention
2 Calusena lansium extract 60% of embodiment, maltodextrin 15%, D-sorbite 20%, mangosteen powder 5%.
Embodiment 15: solid beverage of the present invention
3 Calusena lansium extract 70% of embodiment, maltodextrin 10%, D-sorbite 18%, mangosteen powder 2%.
Embodiment 16: solid beverage of the present invention
4 Calusena lansium extract 70% of embodiment, maltodextrin 5%, D-sorbite 5%, mangosteen powder 10%.
Embodiment 17: solid beverage of the present invention
5 Calusena lansium extract 59% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 1%.
Embodiment 18: solid beverage of the present invention
6 Calusena lansium extract 50% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 10%.
Embodiment 19: solid beverage of the present invention
7 Calusena lansium extract 60% of embodiment, maltodextrin 15%, D-sorbite 20%, mangosteen powder 5%.
Embodiment 20: solid beverage of the present invention
8 Calusena lansium extract 70% of embodiment, maltodextrin 10%, D-sorbite 18%, mangosteen powder 2%.
Embodiment 21: solid beverage of the present invention
9 Calusena lansium extract 70% of embodiment, maltodextrin 5%, D-sorbite 5%, mangosteen powder 10%.
Embodiment 22: solid beverage of the present invention
10 Calusena lansium extract 59% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 1%.
Embodiment 23: solid beverage of the present invention
11 Calusena lansium extract 50% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 10%.
Embodiment 24: solid beverage of the present invention
12 Calusena lansium extract 60% of embodiment, maltodextrin 15%, D-sorbite 20%, mangosteen powder 5%.
Embodiment 25: solid beverage of the present invention
13 Calusena lansium extract 70% of embodiment, maltodextrin 10%, D-sorbite 18%, mangosteen powder 2%.
Embodiment 26: solid beverage of the present invention
14 Calusena lansium extract 70% of embodiment, maltodextrin 5%, D-sorbite 5%, mangosteen powder 10%.
Embodiment 27: solid beverage of the present invention
15 Calusena lansium extract 59% of embodiment, maltodextrin 20%, D-sorbite 20%, mangosteen powder 1%.
Embodiment 28: the influence to diabetes B rat
Experimental animal: SPF grades of healthy male SD rats, 6~8 week old, weight (140 ± 20) g.
Trial drug: solid beverage made from extract made from the embodiment of the present invention 1~12 and embodiment 13~27；
Contrast groups: metformin hydrochloride tablet.
Modeling: after rat is fed high glucose and high fat feed 4 weeks, prepared by diabetes B model to rat injection STZ: before modeling
Rat Fast can't help water 12h, and STZ is dissolved in citric acid-sodium citrate solution, rat is injected intraperitoneally, rat STZ
Injection dosage be 50mg/kg.Rat fasting blood-glucose is measured after 1 week, blood glucose value >=9mmol/L is as rat model.
Grouping administration: diabetes rat is randomly divided into 89 groups: model group (distilled water, isometric), melbine group (salt
Sour diformin tablet, 0.1g/kg), high, medium and low dosage group (10g/kg, the 5g/ of extract or solid beverage provided by the invention
Kg, 1g/kg), every group 8；Separately take 8 normal rats as blank group (distilled water, isometric).Continuous gavage be administered 28d, 1
Secondary/d, the feeding of blank group rat maintains feed during administration, remaining each group feeds high glucose and high fat feed.All Rat Fasts are not
Prohibit water 8h, cuts tail point and take blood, blood glucose meter measures fasting blood sugar.The results are shown in Table 1.
The influence (n=8, mmol/L) of the extract provided by the invention of table 1 or solid beverage to diabetes B rat FBG
Note: compared with the blank group same period,*P<0.01；Compared with the model group same period,△P<0.01；Ratio before being treated with this group
Compared with,▲P<0.01。
The high, medium and low dosage group of solid beverage (10g/kg, 5g/kg, 1g/kg) of the present invention and positive drug melbine
Group has preferable hypoglycemic effect (P < 0.01) to diabetes rat, wherein extract provided by the invention or solid drink
Material is high, middle dose group is more excellent than control group effect.
Embodiment 29: external alpha-glucosaccharase enzyme inhibition activity test
Trial drug: extract or solid beverage provided by the invention；Contrast groups: acarbose.
Reagent: acarbose, alpha-glucosidase, p-nitrophenyl-alpha-glucosaccharase (pNPG), alpha-glucosidase, phosphorus
Potassium hydrogen phthalate, potassium dihydrogen phosphate, dehydrated alcohol, dimethyl sulfoxide and sodium carbonate.
Method:: kaliumphosphate buffer (pH value 6.8) 0.5mL of 0.1mmol/L adds 10mg/L alpha-glucosidase
100 μ L, 0.5mL sample solutions mix, and after 37 DEG C of constant temperature 15min, 2.5mmol/L pNPG 0.5mL are added, 37 DEG C after mixing
Constant temperature 15min is eventually adding the Na of 1mL 0.2mol/L2CO3Solution terminates reaction, and OD value is surveyed under 405nm wavelength, repeats 3
It is secondary, it is averaged.Experiment is repeated 3 times.Using acarbose as positive control, respectively detect different quality concentration (0.05g/mL,
0.1g/mL and 0.5g/mL) 5 solid beverage of the embodiment of the present invention to the inhibitory activity of alpha-glucosidase, based on (formula 1)
Inhibiting rate is calculated, and calculates its IC50Value.As a result as shown in table 2 below.
Inhibition of enzyme activity rate=[ABlank-(ASample-ABackground)]/ABlank× 100% (formula 1)
ABlank: sample to be tested is not added, the solvent of sample to be tested, the light absorption value after being reacted only is added
ASample: the light absorption value after sample to be tested reaction is added
ABackground: the light absorption value after only adding sample to be tested reaction
The external alpha-glucosaccharase enzyme inhibition activity test effect of the solid beverage of the present invention of table 2
Note: compared with acarbose,*P<0.05；#P<0.01。
It is above-mentioned the experimental results showed that, solid beverage of the present invention has apparent alpha-glucosaccharase enzyme inhibition activity to act on, and inhibits
Effect is considerably better than positive controls acarbose (p < 0.01, P < 0.05).
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. the extracting method of blood sugar reducing component in Calusena lansium, which is characterized in that after taking Calusena lansium to crush, after water extract-alcohol precipitation or alcohol extracting,
Extracting solution is collected in filtering；
The water extract-alcohol precipitation specifically: take smashed Calusena lansium, add water heating and refluxing extraction, collect Aqueous extracts, be concentrated into feed liquid
It than 1:1, is slow added into ethyl alcohol and carries out alcohol precipitation, collect supernatant liquid filtering, obtain precipitation solution, concentration；
The alcohol extracting specifically: take smashed Calusena lansium to mix heated refluxing extraction with ethyl alcohol, collect alcohol extract, filtering is dense
Contracting；
The water extracts or ethyl alcohol extraction stands alone as heating and refluxing extraction or ultrasonic extraction；
The temperature of the heating and refluxing extraction is 70 DEG C~100 DEG C, and the time is 1.5h~3h, and number is 3~4 times；
The frequency of the ultrasonic extraction is 60W/L, and the time is 1h~3h, and number is 4~6 times.
2. extracting method as described in claim 1, which is characterized in that in the water extract-alcohol precipitation, smashed Calusena lansium and water
Mass ratio be 1:(5~15)；The volume ratio of the Aqueous extracts and ethyl alcohol is 1:1；
In the alcohol extracting smashed Calusena lansium and ethyl alcohol mass ratio be 1:(5~10)；
The concentration of ethyl alcohol is 75%~95% in the alcohol extracting；
The water extract-alcohol precipitation concentration is 50%~70%.
3. extracting method as claimed in claim 1 or 2, which is characterized in that further include the steps that concentration after the filtering；It is described
Thickening temperature is 35 DEG C~50 DEG C, and vacuum degree 50hPa~200hPa is concentrated into solid-liquid ratio 1:1~1:1.3.
4. extracting method as claimed in claim 3, which is characterized in that further include the steps that freeze-drying, institute after the concentration
The temperature for stating vacuum freeze drying is -50 DEG C~-10 DEG C, and vacuum degree is 1.3Pa~13Pa.
5. the extract as made from Claims 1-4 described in any item extracting methods.
6. extract as claimed in claim 5 is preparing the application in hypoglycemic drug, food or drink.
7. drink, which is characterized in that including extract as claimed in claim 5 and common auxiliary material.
8. drink as claimed in claim 7, which is characterized in that the auxiliary material includes maltodextrin, D-sorbite and Siraitia grosvenorii
Powder；
In terms of mass parts, the drink has following component to be made:
9. drug, which is characterized in that including extract as claimed in claim 5 and pharmaceutically acceptable auxiliary material.
10. food, which is characterized in that including acceptable auxiliary material in extract as claimed in claim 5 and food.
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| CN112450396A (en) * | 2020-11-18 | 2021-03-09 | 广州合诚三先生物科技有限公司 | Clausena lansium compound composition and preparation method and application thereof |
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| CN105012452A (en) * | 2014-04-30 | 2015-11-04 | 周亚伟 | New application of clausena lansium leaves |
| CN109568420A (en) * | 2017-09-29 | 2019-04-05 | 澳门大学 | The purposes of Chinese wampee seed and its extract in the food, health care product or drug of preparation treatment diabetes, obesity and hyperlipidemia |
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Application publication date: 20191015 |