CN110314557A - A kind of bio-pharmaceuticals nanofiber coating sterilization film and preparation method thereof - Google Patents

A kind of bio-pharmaceuticals nanofiber coating sterilization film and preparation method thereof Download PDF

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Publication number
CN110314557A
CN110314557A CN201910658905.0A CN201910658905A CN110314557A CN 110314557 A CN110314557 A CN 110314557A CN 201910658905 A CN201910658905 A CN 201910658905A CN 110314557 A CN110314557 A CN 110314557A
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nanofiber
bio
pharmaceuticals
sterilization film
thin layer
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Inventor
刘轲
王栋
王旭
程盼
郭启浩
李沐芳
鲁振坦
赵青华
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Wuhan Textile University
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Wuhan Textile University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0002—Organic membrane manufacture
    • B01D67/0006—Organic membrane manufacture by chemical reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/009—After-treatment of organic or inorganic membranes with wave-energy, particle-radiation or plasma
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/14—Membrane materials having negatively charged functional groups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/36—Hydrophilic membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/40—Fibre reinforced membranes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection

Abstract

The invention discloses a kind of bio-pharmaceuticals nanofiber coating sterilization films and preparation method thereof, the bio-pharmaceuticals nanofiber coating sterilization film includes the Nanofiber Network thin layer on nonwoven substrate and its surface, is crosslinked between the nonwoven substrate and the Nanofiber Network thin layer by chemical cross-linking agent；Electronegativity functional group is contained on the nanofiber and micrometer fibers surface；The present invention effectively intercepts bacterium by coating the lesser Nanofiber Network thin layer in aperture on nonwoven substrate surface；And by carrying out Atmosphere Plasma processing, the charge species of film surface are regulated and controled, to improve the hydrophily and anti-protein adsorption of film, obtain that a kind of degerming for being able to satisfy bio-pharmaceuticals demand is high-efficient, sterilization film of good separating effect, and degerming membrane structure is simple, easily prepared, environmental pollution, being capable of industrialized production.

Description

A kind of bio-pharmaceuticals nanofiber coating sterilization film and preparation method thereof
Technical field
The present invention relates to technical field of membrane separation, more particularly to a kind of bio-pharmaceuticals nanofiber coating sterilization film and Preparation method.
Background technique
In field of biological pharmacy, since the requirement to purity is very high, bio-separation process will have a direct impact on bio-pharmaceuticals production Quality, yield and the cost of product, are the key that bio-pharmaceuticals.Compared with the separation of conventional chemical products, bio-separation will guarantee High-purity and bioactivity, it usually needs low temperature, cleaning, do not change product bioactivity under conditions of carry out, and UF membrane Technology as a kind of low consumption, efficiently, can low-temperature operation isolation technics, can be realized the high efficiency extraction under room temperature to drug, it is existing It is widely used to biomedicine field.Simultaneously as the separating effect of membrane separation technique and the performance of film used are closely related, The performance of microfiltration membranes for the initial link of pharmaceutical operations has key effect to the quality-improving of biomedical product.
In recent years, high-performance microfiltration membranes have been increasingly becoming most wide application surface, dosage maximum, the most rapid one kind of market development High-performance seperation film is mainly used for removing the virus having a size of 0.01-10 μm, bacterium and particle in bio-pharmaceuticals engineering, While guaranteeing drug bioactivity, the removal of the microorganisms such as bacterium, therefore also referred to as sterilization film are realized.Due to cell factor, Requirement of the pharmaceutical grade proteins such as the antibody and enzyme product to purity is very high, and separation process needs to remove the bacterium in fermentation process The nuisances such as body, cell fragment, virus, heat source and foreign protein or dud guarantee separative efficiency；It requires simultaneously to drug The activity of effective component do not generate destructions, guarantee yield；However, existing sterilization film is all difficult to reach the height of practical bio-pharmaceuticals Standard.
Currently, sterilization film mainly uses phase inversion or biaxial tension method to prepare: on the one hand, membrane material obtained have compared with Big single hole size, for realizing higher flux, and high rejection mainly passes through thickness (tens arrive several hundred microns) direction In-depth filtration realizes that degerming is inefficient；On the other hand, since common diaphragm material is mainly the hydrophobicitys such as PVDF, PES The material of the polar functionalities such as material or PA is carrying out the degerming of pharmacy stoste (aqueous phase system) in application, there are surface hydrophilics Property it is insufficient, anti-albumen adhesiveness is poor the problems such as.
The patent of Publication No. CN106362601A discloses a kind of nano fibrous membrane filtering material with antibacterial functions And preparation method thereof, by being adhered to Nanowire in nonwoven substrate surface coated with nano fiber, and by hydrophilic antibiotic agent Dimension table face, so that the excellent nano fibrous membrane filtering material of good anti-bacterial effect, filter effect be made；This method is hydrophilic by adding Property antibacterial agent carry out degerming, although preferable hydrophily can be shown, and be able to suppress bacterium in the growth of film surface, resist Microbial inoculum can equally generate certain influence to the effective component of drug, meanwhile, which does not simultaneously solve anti-albumen adhesiveness Difference problem, then the filtering material is only applicable to carry out the filtering of conventional chemical field, and may not apply in bio-pharmaceuticals into Row degerming.Therefore, currently still lack can be used for bio-pharmaceuticals degerming is high-efficient, good hydrophilic property, anti-albumen adhesiveness is high removes Mycoderm.
Summary of the invention
It is an object of the invention in view of the above-mentioned problems, provide a kind of bio-pharmaceuticals nanofiber coating sterilization film and its Preparation method carries out bacterium effective by coating the lesser Nanofiber Network thin layer in aperture on nonwoven substrate surface It intercepts；And by carrying out Atmosphere Plasma processing, the charge species of film surface are regulated and controled, to improve the hydrophily of film With anti-protein adsorption, obtain that degerming is high-efficient, sterilization film of good separating effect.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of bio-pharmaceuticals nanofiber coating sterilization film, including nonwoven substrate and be covered on described non-woven The Nanofiber Network thin layer on cloth base material surface, passing through between the nonwoven substrate and the Nanofiber Network thin layer Crosslinking agent is learned to be crosslinked；The nonwoven substrate is crosslinked by micrometer fibers by chemical cross-linking agent, the Nanowire Dimension network thin layer is crosslinked by nanofiber by chemical cross-linking agent, and the surface of the micrometer fibers and the nanofiber is equal Contain electronegativity functional group.
Further, the surface density of the Nanofiber Network thin layer is 6-10g/m2, the face of the nonwoven substrate Density is 40-150g/m2。
Further, the Nanofiber Network thin layer with a thickness of 2-10 μm；The Nanofiber Network thin layer is put down Equal aperture is 100-140nm, and maximum diameter of hole is not more than 350nm.
Further, the ingredient of the nanofiber is one in PVA-co-PE or PP or PA or PET or PBT or PTT Kind, the diameter of the nanofiber is 50-300nm.
Further, the ingredient of the nonwoven substrate be polypropylene or one of polyester or polyamide or a variety of, The structure of the nonwoven substrate is one of melt-blown, spunbond, two-component spunbond or a variety of compound；The non-woven cloth The average pore size of substrate is 1-20 μm, and maximum diameter of hole is less than 50 μm.
Further, the chemical cross-linking agent is one of organic silicon, polyalcohols, glycidol ether, glutaraldehyde.
Further, carboxyl (- COOH) or sulfonic group (- SO are contained in the surface of the nanofiber and micrometer fibers3H) electric One of negativity functional group.
Further, the Nanofiber Network thin layer can intercept in water phase including defective Pseudomonas alba All bacteriums, nanofiber coating sterilization film is to the adsorption rate of bovine serum albumin less than 0.5%.
It is a further object of the present invention to provide a kind of preparation sides of bio-pharmaceuticals nanofiber coating sterilization film Method includes the following steps:
(1) nanofiber is prepared using melt blending phase separation method；
(2) isopropanol and deionized water are dispersed according to the ratio of 5-50g/L by nanofiber obtained in step (1) Mass ratio is that the chemical crosslinking for accounting for nanofiber gross mass 0.1%-3% is added in mixed solution in the mixed solution of 1:1 Agent stirs evenly, and prepares the nanofiber suspension that nanofibers solid content is 0.5wt%-5wt%, is sealed；
(3) nanofiber suspension by step (2) preparation is coated on nonwoven substrate, after air drying, i.e., Obtain nanofiber coating sterilization film；
(4) 30-90min is first impregnated into the nanofiber coating degerming of step (3) preparation in concentrated base, then with water and ethyl alcohol Cleaning several times, air-dries at normal temperature；1-30min is handled through Atmosphere Plasma again, is sealed and is used to get to bio-pharmaceuticals Nanofiber coating sterilization film.
Further, the concentrated base is NaOH, KOH, NH3·H2O、Ba(OH)2One of aqueous solution, the concentrated base Mass fraction is 10%-30%.
Further, the atmosphere is one or both of air, oxygen, sulfur dioxide, and the air pressure of the atmosphere is 10kPa-200kPa, the intensity of the plasma are 50-200W.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention utilizes nanometer by coating the lesser Nanofiber Network thin layer in aperture on nonwoven substrate surface The advantages that fiber aperture is small, porosity is high, uniformity is good, not only ensure that high throughput, but also realize effective interception to bacterium, remove Bacterium is high-efficient, and has extremely low protein adsorption rate；
2, bio-pharmaceuticals provided by the invention is in nanofiber coating sterilization film, nonwoven substrate and nanometer fiber net Between nanofiber between micrometer fibers between network thin layer, in nonwoven substrate and in Nanofiber Network thin layer Be attached and combined by chemical cross-linking agent, using the functional group in chemical cross-linking agent as abutment by nanofiber, micron Polymerization macromolecules cross-linking between fiber gets up, and forms network-like structure, and the binding force between reinforcing fiber makes coating securely may be used It leans on, it is not easily to fall off, and improve the contamination resistance of film layer；
3, of the invention by further enhancing the connection effect between film layer to sterilization film progress atmosphere plasma treatment, and The charge species of film surface are regulated and controled, film surface is made to contain electronegativity functional group, and most of protein all has negative electricity Lotus so that sterilization film be made to have extremely low protein adsorption rate, and improves the hydrophily of film；
4, bio-pharmaceuticals provided by the invention is simple with nanofiber coating degerming membrane structure, easily prepared, has higher Degerming efficiency, anti-protein adsorption performance and hydrophily it is stronger, be able to satisfy the demand of bio-pharmaceuticals, and prepare and use process ring Protect it is pollution-free, being capable of industrialized production.
Detailed description of the invention
Fig. 1 is the optical imagery that the water sample containing bacterium is coated on afterwards before filtration on plate, wherein a is the water containing bacterium The optical imagery being coated on plate before spline filter, b are the same water sample containing bacterium through Nanowire made from the embodiment of the present invention 1 Tie up the optical imagery being coated on plate after coating sterilization film filters.
Specific embodiment
With reference to embodiments, the present invention will be described in further detail, so that advantages and features of the invention can be more It is easy to be readily appreciated by one skilled in the art, so as to make a clearer definition of the protection scope of the present invention.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiment of the present invention, Those of ordinary skill in the art's obtained all other embodiment without making creative work belongs to this Invent protected range.
Embodiment 1
It present embodiments provides a kind of bio-pharmaceuticals nanofiber coating sterilization film, including nonwoven substrate and covers Cover the Nanofiber Network thin layer on the nonwoven substrate surface, the nonwoven substrate and the Nanofiber Network It is crosslinked between thin layer by chemical cross-linking agent；The nonwoven substrate is crosslinked by micrometer fibers by chemical cross-linking agent At the Nanofiber Network thin layer is crosslinked by nanofiber by chemical cross-linking agent, and the nanofiber and micron are fine Contain carboxyl functional group in dimension table face.
Wherein, the composition of the nanofiber is PVA-co-PE；The composition of the nonwoven substrate is polypropylene, knot Structure is melt-blown, and average pore size is 20 μm, and maximum diameter of hole is 50 μm, surface density 40g/m2；The chemical cross-linking agent is penta 2 Aldehyde.
It is a further object of the present invention to provide a kind of preparation sides of bio-pharmaceuticals nanofiber coating sterilization film Method includes the following steps:
(1) nanofiber is prepared using melt blending phase separation method: by PVA-co-PE and acetylbutyrylcellulose (CAB) 2:3 is uniformly mixed in mass ratio, is squeezed out, is granulated in the double screw extruder that processing temperature is 200 DEG C, and PVA- is made Co-PE/CAB composite material passes it through melt spinning machine and carries out spinning, drawing-off, obtains composite fibre, then in acetone by it Reflux, control reflux temperature are 60 DEG C, and return time 72h extracts acetylbutyrylcellulose, obtains not after drying at normal temperature With the nanofiber of diameter, diameter range 50-300nm；
(2) disperse nanofiber obtained in step (1) according to the ratio of 5g/L the matter of isopropanol and deionized water Amount is than stirring for the chemical cross-linking agent for accounting for nanofiber gross mass 0.1% is added in mixed solution in the mixed solution of 1:1 Uniformly, the nanofiber suspension that nanofibers solid content is 0.5wt% is prepared, is sealed；
(3) nanofiber suspension by step (2) preparation is coated on the polypropylene nonwoven substrate of melt-blown, coating With a thickness of 4 μm, coating surface density is 6g/m2, to get nanofiber coating sterilization film after air drying；
(4) the nanofiber coating degerming of step (3) preparation is first soaked in the NaOH aqueous solution that mass fraction is 10% 30min is steeped, then is cleaned three times with water and ethyl alcohol, is air-dried at normal temperature；Again under the air atmosphere of 20kPa, plasma is controlled Intensity is 50W, handles 10min, then is sealed to get bio-pharmaceuticals nanofiber coating sterilization film is arrived.
Test can be carried out to the strainability and adsorption of protein of nanofiber coating sterilization film obtained, wherein egg White matter absorption property is tested by taking bovine serum albumin as an example；The average pore size for measuring Nanofiber Network thin layer is 100nm, most Large aperture is 250nm, bovine serum albumin adsorption rate < 0.5%.
Nanofiber coating sterilization film obtained is reused, the water sample containing defective Pseudomonas alba is filtered, And the water sample of filtering front and back is respectively coated on plate, it is observed using microscope, as a result as shown in Figure 1.It can be with by Fig. 1 Find out, a large amount of bacteriums are contained in the water sample before filtering, and after the filtering of the nanofiber coating sterilization film made from the present embodiment, water Bacterium residual is not observed in sample, degerming efficiency reaches 100%.
Embodiment 2~5
Embodiment 2~5 provides a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film, with embodiment 1 It compares, the difference is that changing concentration of the nanofiber in isopropanol and the mixed solution of deionized water in step (2) With the additive amount of chemical cross-linking agent, the concentration of the corresponding nanofiber of each embodiment and the additive amount of crosslinking agent are as shown in table 1:
The additive amount of each embodiment of table 1 corresponding nanofiber concentration and crosslinking agent
To the strainability of nanofiber coating sterilization film made from each embodiment, degerming performance and adsorption of protein energy Tested, the average pore size of the Nanofiber Network thin layer measured, maximum diameter of hole, defective Pseudomonas alba degerming efficiency The results are shown in Table 2 with bovine serum albumin adsorption rate:
The performance of nanofiber coating sterilization film made from each embodiment of table 2
Embodiment Average pore size (nm) Maximum diameter of hole (nm) Degerming efficiency (%) Protein adsorption rate (%)
Embodiment 2 100 230 100 <0.5
Embodiment 3 100 220 100 <0.5
Embodiment 4 100 210 100 <0.5
Embodiment 5 100 200 100 <0.5
As can be seen from Table 2, concentration and change of the nanofiber in isopropanol and the mixed solution of deionized water are changed The additive amount for learning crosslinking agent, can not influence the degerming performance and adsorption of protein of nanofiber coating sterilization film, but right The maximum diameter of hole of membrane filtration characteristic has a certain impact, and with the increase of nanofiber and content of crosslinking agent in mixed solution, The maximum diameter of hole of sterilization film obtained is gradually reduced, membrane filtration characteristic enhancing.
Embodiment 6~8
Embodiment 6~8 provides a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film, with embodiment 1 It compares, the difference is that changing the thickness for the Nanofiber Network thin layer being coated on nonwoven substrate in step (3) And surface density, the thickness and surface density of the corresponding Nanofiber Network thin layer of each embodiment are as shown in table 3:
The thickness and surface density of the corresponding Nanofiber Network thin layer of each embodiment of table 3
Embodiment Nanofiber Network thickness of thin layer (μm) Nanofiber Network thin layer surface density (g/m2)
Embodiment 6 2 7
Embodiment 7 7 8
Embodiment 8 10 10
To the strainability of nanofiber coating sterilization film made from each embodiment, degerming performance and adsorption of protein energy Tested, the average pore size of the Nanofiber Network thin layer measured, maximum diameter of hole, defective Pseudomonas alba degerming efficiency The results are shown in Table 4 with bovine serum albumin adsorption rate:
The performance of nanofiber coating sterilization film made from each embodiment of table 4
As can be seen from Table 4, the thickness coated in the Nanofiber Network thin layer on nonwoven substrate and face are changed Density can not influence nanofiber coating sterilization film degerming performance and adsorption of protein, but most to membrane filtration characteristic Large aperture has a certain impact, and with the increase of Nanofiber Network thickness of thin layer and surface density, sterilization film obtained is most Large aperture is gradually reduced, when Nanofiber Network thickness of thin layer is 10 μm, surface density 10g/m2When, sterilization film obtained is most Large aperture is only 160nm, has excellent membrane filtration characteristic.
Embodiment 9~10
Embodiment 9~10 provides a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film, with embodiment 1 It compares, the difference is that changing type, mass fraction and the soaking time of concentrated base in step (4), each embodiment is corresponding The type of concentrated base, concentration and soaking time are as shown in table 5:
Type, concentration and the soaking time of the corresponding concentrated base of each embodiment of table 5
Embodiment Concentrated base type Mass fraction (%) Soaking time (min)
Embodiment 9 KOH 20 60
Embodiment 10 Ba(OH)2 30 90
To the strainability of nanofiber coating sterilization film made from each embodiment, degerming performance and adsorption of protein energy Tested, the average pore size of the Nanofiber Network thin layer measured, maximum diameter of hole, defective Pseudomonas alba degerming efficiency The results are shown in Table 6 with bovine serum albumin adsorption rate:
The performance of nanofiber coating sterilization film made from each embodiment of table 6
Embodiment Average pore size (nm) Maximum diameter of hole (nm) Degerming efficiency (%) Protein adsorption rate (%)
Embodiment 9 100 250 100 <0.5
Embodiment 10 100 250 100 <0.5
As can be seen from Table 6, type, mass fraction and the soaking time for changing concentrated base in a certain range, to Nanowire Strainability, degerming performance and the adsorption of protein of dimension coating sterilization film can not influence, nanofiber coating obtained Sterilization film is still able to maintain preferably performance, and stability is good.
Embodiment 11~13
Embodiment 11~13 provides a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film, with embodiment 1 compares, the difference is that changing the atmosphere type that Atmosphere Plasma is handled in step (4), atmosphere pressure, plasma Intensity and plasma treatment time, the atmosphere type of the corresponding Atmosphere Plasma processing of each embodiment, atmosphere pressure, etc. from Daughter intensity and plasma treatment time are as shown in table 7:
The corresponding atmosphere type of each embodiment of table 7, atmosphere pressure, plasma intensity and processing time
Embodiment Atmosphere type Atmosphere pressure (kPa) Plasma intensity (W) It handles time (min)
Embodiment 11 Oxygen 20 100 30
Embodiment 12 Sulfur dioxide 10 150 5
Embodiment 13 Air 200 200 1
To the strainability of nanofiber coating sterilization film made from each embodiment, degerming performance and adsorption of protein energy Tested, the average pore size of the Nanofiber Network thin layer measured, maximum diameter of hole, defective Pseudomonas alba degerming efficiency The results are shown in Table 8 with bovine serum albumin adsorption rate:
The performance of nanofiber coating sterilization film made from each embodiment of table 8
Embodiment Average pore size (nm) Maximum diameter of hole (nm) Degerming efficiency (%) Protein adsorption rate (%)
Embodiment 11 140 350 100 <0.5
Embodiment 12 130 300 100 <0.5
Embodiment 13 120 300 100 <0.5
As can be seen from Table 8, atmosphere type, the atmosphere pressure, plasma intensity of Atmosphere Plasma processing are changed And plasma treatment time, the degerming performance and adsorption of protein of sterilization film can be had not significant impact, only to sterilization film Strainability have a certain impact, when atmosphere pressure and higher plasma intensity, do not need the too long processing time, i.e., It may know that the relatively smaller sterilization film of average pore size and maximum diameter of hole, strainability is preferable.
Embodiment 14~15
Embodiment 14~15 provides a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film, with embodiment 1 compares, the difference is that changing the type of nanofiber, nonwoven substrate and crosslinking agent, each embodiment is corresponding to be received The type of rice fiber, nonwoven substrate and crosslinking agent is as shown in table 9:
The type of the corresponding nanofiber of each embodiment of table 9, nonwoven substrate and crosslinking agent
To the strainability of nanofiber coating sterilization film made from each embodiment, degerming performance and adsorption of protein energy Tested, the average pore size of the Nanofiber Network thin layer measured, maximum diameter of hole, defective Pseudomonas alba degerming efficiency The results are shown in Table 10 with bovine serum albumin adsorption rate:
The performance of nanofiber coating sterilization film made from each embodiment of table 10
Embodiment Average pore size (nm) Maximum diameter of hole (nm) Degerming efficiency (%) Protein adsorption rate (%)
Embodiment 14 120 270 100 <0.5
Embodiment 15 100 250 100 <0.5
As can be seen from Table 10, the type for changing nanofiber, nonwoven substrate and crosslinking agent, to the property of sterilization film It can not have much affect, it is different types of to show that bio-pharmaceuticals nanofiber coating sterilization film provided by the invention can use Prepared by nanofiber, nonwoven substrate and crosslinking agent, the scope of application is wider, and sterilization film obtained all has preferably Strainability, degerming performance and adsorption of protein, can satisfy the demand of practical application.
The above, only of the invention illustrates embodiment, not to the present invention in any form with substantial limitation, It should be pointed out that for those skilled in the art, under the premise of not departing from the method for the present invention, that makes several changes It also should be regarded as protection scope of the present invention into supplement；Meanwhile all substantial technologicals according to the present invention do above-described embodiment Any equivalent variations change, modification and differentiation, still fall within protection scope of the present invention.

Claims (11)

1. a kind of bio-pharmaceuticals nanofiber coating sterilization film, it is characterised in that: including nonwoven substrate and be covered on The Nanofiber Network thin layer on the nonwoven substrate surface, the nonwoven substrate and the Nanofiber Network thin layer Between be crosslinked by chemical cross-linking agent；The nonwoven substrate is crosslinked by micrometer fibers by chemical cross-linking agent, The Nanofiber Network thin layer is crosslinked by nanofiber by chemical cross-linking agent, the micrometer fibers and the Nanowire Contain electronegativity functional group in the surface of dimension.
2. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the nanometer The surface density of network of fibers thin layer is 6-10g/m2, the surface density of the nonwoven substrate is 40-150g/m2。
3. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the nanometer Network of fibers thin layer with a thickness of 2-10 μm；The average pore size of the Nanofiber Network thin layer is 100-140nm, maximum diameter of hole No more than 350nm.
4. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the nanometer The ingredient of fiber is PVA-co-PE or PP or PA or PET or one of PBT or PTT, and the diameter of the nanofiber is 50- 300nm。
5. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: described non-to knit The ingredient for making cloth base material is polypropylene or one of polyester or polyamide or a variety of, and the structure of the nonwoven substrate is molten One of spray, spunbond, two-component spunbond are a variety of compound；The average pore size of the nonwoven substrate is 1-20 μm, most Large aperture is less than 50 μm.
6. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the chemistry Crosslinking agent is one of organic silicon, polyalcohols, glycidol ether, glutaraldehyde.
7. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the nanometer Contain carboxyl (- COOH) or sulfonic group (- SO in the surface of fiber and micrometer fibers3H) one of electronegativity functional group.
8. a kind of bio-pharmaceuticals nanofiber coating sterilization film according to claim 1, it is characterised in that: the nanometer Network of fibers thin layer can intercept all bacteriums in water phase including defective Pseudomonas alba, nanofiber coating degerming Film is to the adsorption rate of bovine serum albumin less than 0.5%.
9. a kind of preparation method of bio-pharmaceuticals described in claim 1 nanofiber coating sterilization film, which is characterized in that packet Include following steps:
(1) nanofiber is prepared using melt blending phase separation method；
(2) disperse nanofiber obtained in step (1) according to the ratio of 5-50g/L the quality of isopropanol and deionized water Than the chemical cross-linking agent for accounting for nanofiber gross mass 0.1%-3% being added in mixed solution, stirs in the mixed solution for 1:1 The nanofiber suspension for uniformly preparing that nanofibers solid content is 0.5wt%-5wt% is mixed, is sealed；
(3) nanofiber suspension by step (2) preparation is coated on nonwoven substrate, is received after air drying Rice fiber coat sterilization film；
(4) the nanofiber coating degerming of step (3) preparation is first impregnated into concentrated base 30-90min, then is cleaned with water and ethyl alcohol Several times, it air-dries at normal temperature；1-30min is handled through Atmosphere Plasma again, is sealed to get to bio-pharmaceuticals nanometer Fiber coat sterilization film.
10. a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film according to claim 9, feature exist In: the concentrated base is NaOH, KOH, NH3·H2O、Ba(OH)2One of aqueous solution, the mass fraction of the concentrated base are 10%- 30%.
11. a kind of preparation method of bio-pharmaceuticals nanofiber coating sterilization film according to claim 9, feature exist In: the atmosphere is one or both of air, oxygen, sulfur dioxide, and the air pressure of the atmosphere is 10kPa-200kPa, institute The intensity for stating plasma is 50-200 W.
CN201910658905.0A 2019-07-19 2019-07-19 A kind of bio-pharmaceuticals nanofiber coating sterilization film and preparation method thereof Pending CN110314557A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111644080A (en) * 2020-06-03 2020-09-11 武汉纺织大学 High-hydrophilicity nanofiber coating-based nanofiltration membrane and preparation method thereof
CN112043024A (en) * 2020-09-11 2020-12-08 无锡泰伯服饰有限公司 One-way moisture-conducting antibacterial mask
CN112246112A (en) * 2020-09-07 2021-01-22 武汉纺织大学 Anti-fouling and antibacterial nanofiber filtering membrane and preparation method thereof
CN113144913A (en) * 2020-05-08 2021-07-23 武汉纺织大学 High-permeability anti-fouling nanofiber separation membrane material and preparation method and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090096114A (en) * 2008-03-07 2009-09-10 경북대학교 산학협력단 Air filter consisted polymethylmethacrylate nanofiber polyethylene terephthalate nonwoven fiber and processing method thereof
CN106362601A (en) * 2016-09-28 2017-02-01 扬州云彩新材料科技有限公司 Nano fiber membrane filtering material with antibacterial function and preparation method thereof
CN106478975A (en) * 2016-10-21 2017-03-08 苏州科技大学 The preparation method of difunctional block polymer and its method for modified polyvinilidene fluoride micro-filtration membrane
CN106668954A (en) * 2017-02-10 2017-05-17 烟台正海生物科技股份有限公司 Antibacterial cation-modified absorbable pachymeninx repair material and preparation method and application thereof
CN107137979A (en) * 2017-05-11 2017-09-08 武汉纺织大学 A kind of micrometer fibers three-dimensional framework/polymer nanofiber composite filter material and preparation method thereof
CN107335346A (en) * 2017-08-01 2017-11-10 东华大学 A kind of bacteria cellulose nanofiber composite filter membrane and preparation method thereof
CN109046040A (en) * 2018-08-03 2018-12-21 武汉纺织大学 Gradient filtration membrane material based on nanofiber and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090096114A (en) * 2008-03-07 2009-09-10 경북대학교 산학협력단 Air filter consisted polymethylmethacrylate nanofiber polyethylene terephthalate nonwoven fiber and processing method thereof
CN106362601A (en) * 2016-09-28 2017-02-01 扬州云彩新材料科技有限公司 Nano fiber membrane filtering material with antibacterial function and preparation method thereof
CN106478975A (en) * 2016-10-21 2017-03-08 苏州科技大学 The preparation method of difunctional block polymer and its method for modified polyvinilidene fluoride micro-filtration membrane
CN106668954A (en) * 2017-02-10 2017-05-17 烟台正海生物科技股份有限公司 Antibacterial cation-modified absorbable pachymeninx repair material and preparation method and application thereof
CN107137979A (en) * 2017-05-11 2017-09-08 武汉纺织大学 A kind of micrometer fibers three-dimensional framework/polymer nanofiber composite filter material and preparation method thereof
CN107335346A (en) * 2017-08-01 2017-11-10 东华大学 A kind of bacteria cellulose nanofiber composite filter membrane and preparation method thereof
CN109046040A (en) * 2018-08-03 2018-12-21 武汉纺织大学 Gradient filtration membrane material based on nanofiber and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
徐铜文编著: "《膜化学与技术教程》", 31 December 2003, 合肥：中国科学技术大学出版社 *
蔺爱国等编著: "《新型功能膜技术及其应用》", 30 November 2013, 东营：中国石油大学出版社 *
郝玛•辛格等著: "《等离子体雷达散射截面减缩技术》", 31 October 2018, 北京：国防工业出版社 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113144913A (en) * 2020-05-08 2021-07-23 武汉纺织大学 High-permeability anti-fouling nanofiber separation membrane material and preparation method and application thereof
CN113144913B (en) * 2020-05-08 2022-08-02 武汉纺织大学 High-permeability anti-fouling nanofiber separation membrane material and preparation method and application thereof
CN111644080A (en) * 2020-06-03 2020-09-11 武汉纺织大学 High-hydrophilicity nanofiber coating-based nanofiltration membrane and preparation method thereof
CN112246112A (en) * 2020-09-07 2021-01-22 武汉纺织大学 Anti-fouling and antibacterial nanofiber filtering membrane and preparation method thereof
CN112246112B (en) * 2020-09-07 2022-04-08 武汉纺织大学 Anti-fouling and antibacterial nanofiber filtering membrane and preparation method thereof
CN112043024A (en) * 2020-09-11 2020-12-08 无锡泰伯服饰有限公司 One-way moisture-conducting antibacterial mask
CN112043024B (en) * 2020-09-11 2024-02-06 无锡泰伯服饰有限公司 One-way moisture-conducting antibacterial mask

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