Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof
Technical Field
The invention relates to the technical field of biological pesticides, and particularly relates to a photosynthetic bacterium rhodobacter capsulatus strain. In addition, the invention also relates to a microbial inoculum agent comprising the photosynthetic bacterium rhodobacter capsulatus strain, and a preparation method and application thereof.
Background
The bacterial leaf blight of rice is one of important diseases in rice production, is commonly called as plague, is one of main diseases in rice, and is an epidemic disease. The rice bacterial leaf blight is mainly harmful to leaves, causes withering and death of plants, has great influence on the rice yield, and can reduce the yield by about 10 percent generally and 50 to 60 percent or even 90 percent seriously once the rice bacterial leaf blight occurs. The disease is caused by bacteria, and the pathogenic bacteria is xanthomonas oryzae (Xanthomonas oryzae). The primary infection source is mainly diseased grass and diseased grain, and bacteria are spread to the seedling bed through water flow to cause seedling diseases. Early rice seedlings often do not show symptoms after infection and become seedlings with bacteria. Late rice may present symptoms in the three-and four-leaf stage. Flooding in the seedling bed period and in the seedling period, the probability of infection of the seedlings is increased, the more times of flooding and top submerging are increased, the longer the time is, and the higher the bacteria carrying rate of the seedlings is. When the seedlings with the bacterin or the symptomatic seedlings are transplanted to a field, the disease starts to be developed when the disease resistance of the rice plants is reduced at the end stage of tillering, and the rice plants become central disease plants. Then a large amount of bacteria pus is generated at the diseased part of the diseased plant, and the disease is continuously re-infected by water filling and storm propagation, so that the disease is continuously expanded and spread in the field.
The rice bacterial blight mainly overwinter in rice seeds, straws and rice stakes and in nearby soil. And (4) sowing diseased grains, wherein germs can invade through roots and coleoptiles of seedlings. Pathogenic bacteria on diseased straws and rice stakes permeate into water flow when encountering rainwater, seedlings contact with the water with the pathogenic bacteria, and the pathogenic bacteria invade rice bodies from water holes and wounds. The rice straw is used for accelerating germination, covering rice seedlings, binding rice seedlings and the like, which is beneficial to disease spreading. In the rice seedling bed period, because the temperature is low and the bacterial load is less, symptoms can not be seen generally, and the symptoms can not be developed until before and after the booting. Pus discharge on the lesion spots can be re-infected by wind, rain, dew, contact with leaves, etc. Pathogenic bacteria invade through host water pores and wounds to cause diseases. High temperature and raininess, and flooding is most favorable for disease prevalence frequently; improper management of fertilizer and water, biased application of nitrogen fertilizer, deep water irrigation, serial irrigation, flood irrigation or waterlogging of rice fields, are easy to induce disease prevalence; it is more susceptible to diseases.
At present, the prevention and control of rice bacterial blight mainly focuses on agricultural measures such as application of chemical pesticides and breeding of disease-resistant varieties, and the problems of pesticide residue, increase of pest resistance, environmental pollution and the like caused by long-term use of a large amount of chemical pesticides seriously affect the sustainable development of agriculture. And the resistance breeding difficulty is high, the period is long, the corresponding agricultural measures are complex, and the economic investment is large. Biological control is a pest control method which utilizes beneficial organisms or other organisms to eliminate or inhibit harmful organisms, is widely adopted and has good ecological, environmental and social benefits, and is in line with the sustainable development of agriculture due to the advantages of environmental, ecological and human health safety, and is the development trend of current and future plant disease control. In recent years, scholars at home and abroad have made great progress in the fundamental research and application research of photosynthetic bacteria. Many research results prove that the photosynthetic bacteria are rich in various physiological active substances and growth promotion factors, and even extracellular components secreted by the bacteria have stimulation on nitrogen-fixing rhizobia, so that the nitrogen-fixing activity of the bacteria can be obviously improved; and the compound contains substances such as proline, uracil, cytosine and the like, so that the yield of various commercial crops can be increased.
Disclosure of Invention
The invention provides a photosynthetic bacterium capsular rhodobacter strain, a microbial inoculum medicament, and a preparation method and application thereof, and aims to solve the technical problems of high difficulty and high cost in prevention and control of rice bacterial leaf blight in the current rice growth process.
The technical scheme adopted by the invention is as follows:
a photosynthetic bacteria Rhodobacter capsulatus strain is photosynthetic bacteria Rhodobacter capsulatus A12(Rhodobacter capsulatus A12), which is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604.
Further, the photosynthetic bacterium rhodobacter capsulatus strain is cultured on a double-layer solid plate culture medium for 4-8 days to form red round colonies, the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm; the photosynthetic bacteria rhodobacter capsulatus strain presents red color after being cultured in a liquid culture medium for 7 d.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain, which comprises the photosynthetic bacteria capsular red bacterium strain biocontrol microbial inoculum prepared by activating, seed culturing, production culturing and the like.
Further, the bacteria concentration of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain is 5 × 105cfu/mL~5×107cfu/mL。
According to another aspect of the invention, the preparation method of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain comprises the following steps: s1, activation: culturing the preserved strain of the photosynthetic bacterium rhodobacter capsulatus strain by a double-layer plate culture method until a red single colony appears; s2, seed culture: inoculating the red single colony cultured in the step S1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; s3, production culture: inoculating the bacterial liquid obtained in the step S2 according to the inoculation amount of 3% -6% of the total volume of the production medium of the photosynthetic bacteria strain, and culturing to logarithmic phase to obtain the biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacteria strain.
Further, the temperature of the seed culture in the step S2 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria liquid culture medium is 7.0-7.1; in the step S3, the temperature of production and culture is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria strain production culture medium is 7.0-7.1.
According to another aspect of the invention, the biocontrol agent for the photosynthetic bacteria capsular red bacteria strain is prepared by centrifuging the biocontrol agent for the photosynthetic bacteria capsular red bacteria strain and collecting supernatant.
According to another aspect of the invention, there is also provided a preparation method of the biocontrol agent of the above photosynthetic bacterium rhodobacter capsulatus strain, comprising the following steps: fermenting and culturing the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain for 5-7 days, performing centrifugal separation after the culture is finished, collecting supernatant, and filtering by a bacteria filter to obtain the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain.
Further, the conditions of fermentation culture are as follows: the temperature of fermentation culture is 30-35 ℃, and the illumination condition is 3000 Lx-4000 Lx; the conditions of the centrifugal separation were: centrifuging for 5-8 min under the condition of 5000-7000 rpm.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain and a biocontrol agent of the photosynthetic bacteria capsular red bacterium strain, which are applied to the growth promotion of rice or the control of rice bacterial blight.
The invention has the following beneficial effects:
the photosynthetic bacteria capsular red bacterium strain disclosed by the invention has the advantages that the culture process of the photosynthetic bacteria capsular red bacterium A12 is simple, the culture time is short, the cost is low, and the photosynthetic bacteria capsular red bacterium strain can be used for preparing the biocontrol microbial inoculum for controlling the bacterial blight of rice and the biocontrol fermentation liquor by a simple, quick and low-cost operation method.
The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain have the characteristics of environmental friendliness, no toxicity to people and livestock, no phytotoxicity to crops, simple and convenient application and the like, and are not easy to cause drug resistance to diseases and insect pests.
The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain can prevent and control bacterial blight of rice and promote the growth of the rice.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of the biocontrol microbial inoculum of a photosynthetic bacterium rhodobacter capsulatus strain according to a preferred embodiment of the present invention; and
fig. 2 is a schematic diagram of biocontrol agents for the photosynthetic bacteria rhodobacter capsulatus strain of the preferred embodiment of the present invention.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
FIG. 1 is a schematic diagram of the biocontrol microbial inoculum of a photosynthetic bacterium rhodobacter capsulatus strain according to a preferred embodiment of the present invention; fig. 2 is a schematic diagram of biocontrol agents for the photosynthetic bacteria rhodobacter capsulatus strain of the preferred embodiment of the present invention.
The photosynthetic bacteria Rhodobacter capsulatus strain of the present embodiment is a photosynthetic bacteria Rhodobacter capsulatus a12(Rhodobacter capsulatus a12), which is deposited in the chinese typical culture collection center with the collection number of CCTCC No: m2018604. The photosynthetic bacteria capsular red bacterium strain disclosed by the invention has the advantages that the culture process of the photosynthetic bacteria capsular red bacterium A12 is simple, the culture time is short, the cost is low, and the photosynthetic bacteria capsular red bacterium strain can be used for preparing the biocontrol microbial inoculum for controlling the bacterial blight of rice and the biocontrol fermentation liquor by a simple, quick and low-cost operation method.
The photosynthetic bacteria rhodobacter capsulatus strain is photosynthetic bacteria rhodobacter capsulatus A12 strain, is obtained by separating and purifying from soil, is preserved in China center for type culture Collection, and has the preservation number of CCTCC No: m2018604.
The photosynthetic bacterium Rhodobacter capsulatus A12(Rhodobacter capsulatus A12) is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604, the preservation unit address is located in Wuhan university No. 299 in the Wuhan district of Wuhan city, Hubei province, China. The gene sequence is as follows: 1000bp
gtgatgcggcagctacacatgcagtcgagcgagaccttcgggtctagcggcggacgggtgagtaacgcgtgggaacgtgccctttgct acggaatagccccgggaaactgggagtaataccgtatgtgcccttcgggggaaagatttatcggcaaaggatcggcccgcgttggattaggtag ttggtggggtaatggcctaccaagccgacgatccatagctggtttgagaggatgatcagccacactgggactgagacacggcccagactcctac gggaggcagcagtggggaatcttagacaatgggggaaaccctgatctagccatgccgcgtgagcgatgaaggccttagggttgtaaagctctttcaggtgggaagataatgacggtaccaccagaagaagccccggctaactccgtgccagcagccgcggtaatacggagggggctagcgttgttc ggaattactgggcgtaaagcgcacgtaggcggatcagaaagtcagaggtgaaatcccagggctcaaccttggaactgcctttgaaactcctggt cttgaggtcgagagaggtgagtggaattccgagtgtagaggtgaaattcgtagatattcggaggaacaccagtggcgaaggcggctcactggct cgatactgacgctgaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgaatgccagtcgtcggc aggcatgcctgtcggtgacacacctaacggattaagcattccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacgggggcc cgcacaagcggtggagcatgtggtttaattcgaagcaacgcgcagaaccttaccaacccttgacatcgggatcgcggttaccggagacggtttc cttcagttcggctggatcccagacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttcggtt
In the embodiment, the photosynthetic bacterium rhodobacter capsulatus strain is cultured on a double-layer solid plate culture medium for 4-8 days to form red round colonies, the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm; the photosynthetic bacteria rhodobacter capsulatus strain is cultured in a liquid culture medium for 78d to be red. The photosynthetic bacterium Rhodobacter capsulatus A12 strain is a gram-negative bacterium and is identified as Rhodobacter capsulatus A12 through physiological and biochemical identification and molecular biological identification. The main biological characteristics are as follows: culturing for 4-8 days on a double-layer solid plate culture medium to form red round colonies, wherein the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm. The color of the solution was red when cultured in liquid medium for 7 d. Good growth under anaerobic and micro-aerobic conditions. The physiochemical characteristics are V-P reaction and methyl red reaction negative, H2The S reaction, the gelatin liquefaction, the urease test and the indole test are all positive, the optimal growth temperature is 30-35 ℃, and the pH value is 7.
Antagonistic microorganisms reported for the biological control of rice bacterial blight are mainly fungi, bacteria, slime mold, marine microorganisms, wherein the biocontrol bacteria comprise bacillus, pseudomonas and the like. The mechanism of inhibiting the bacterial blight of rice by the photosynthetic bacterium capsular red bacterium A12 is mainly to inhibit the growth of pathogenic bacteria by secreting one or more antibacterial substances such as bacteriocins, proteins, lipopeptides, polypeptides, phenols and the like, thereby preventing the occurrence of plant diseases. In addition, the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus A12 strain induces the plants to generate disease resistance potential, thereby enhancing the disease resistance of the plants. And the photosynthetic bacteria rhodobacter capsulatus strain has some active substances, can inactivate the pathogenicity of pathogens, promote the proliferation of beneficial microorganisms such as actinomycetes and the like, and inhibit the growth of the pathogens, thereby effectively inhibiting the occurrence and spread of certain plant diseases. Therefore, the photosynthetic bacterium capsular red bacterium A12 strain has rich biocontrol resources and wide application prospect in preventing and treating the bacterial blight of rice.
As shown in fig. 1, according to another aspect of the present invention, there is also provided a biocontrol microbial inoculum of a photosynthetic bacteria capsular red bacterium strain, which comprises the photosynthetic bacteria capsular red bacterium strain biocontrol microbial inoculum prepared by activating, seed culturing, production culturing and the like of the photosynthetic bacteria capsular red bacterium strain. The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain have the characteristics of environmental friendliness, no toxicity to people and livestock, no phytotoxicity to crops, simple and convenient application and the like, and are not easy to cause drug resistance to diseases and insect pests. The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain can prevent and control bacterial blight of rice and promote the growth of the rice.
In this example, the concentration of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain is 5 × 105 cfu/mL-5 × 107cfu/mL。
According to another aspect of the invention, the preparation method of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain comprises the following steps: s1, activation: culturing the preserved strain of the photosynthetic bacterium rhodobacter capsulatus strain by a double-layer plate culture method until a red single colony appears; s2, seed culture: inoculating the red single colony cultured in the step S1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; s3, production culture: inoculating the bacterial liquid obtained in the step S2 according to the inoculation amount of 3% -6% of the total volume of the production medium of the photosynthetic bacteria strain, and culturing to logarithmic phase to obtain the biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacteria strain.
In this embodiment, the optimal temperature for seed culture in step S2 is 30 ℃ to 35 ℃, the illumination conditions are 2500Lx to 4000Lx, and the optimal pH of the photosynthetic bacteria liquid culture medium is 7.0 to 7.1. The temperature of the production culture in the step S3 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the optimum pH value of the photosynthetic bacteria strain production culture medium is 7.0-7.1.
As shown in figure 1, according to another aspect of the invention, a biocontrol agent for a photosynthetic bacteria capsular red bacterium strain is also provided, and is prepared by centrifuging the biocontrol agent for the photosynthetic bacteria capsular red bacterium strain and collecting supernatant. According to another aspect of the invention, there is also provided a preparation method of the biocontrol agent of the above photosynthetic bacterium rhodobacter capsulatus strain, comprising the following steps: fermenting and culturing the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain for 5-7 days, performing centrifugal separation after the culture is finished, collecting supernatant, and filtering by a bacteria filter to obtain the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain.
In this example, the conditions of the fermentation culture were: the temperature of fermentation culture is 30-35 ℃, and the illumination condition is 3000 Lx-4000 Lx. The conditions of the centrifugal separation were: centrifuging for 5-8 min under the condition of 5000-7000 rpm.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain and a biocontrol agent of the photosynthetic bacteria capsular red bacterium strain, which are applied to the growth promotion of rice or the control of rice bacterial blight.
Examples
In the following examples, each chemical reagent is commercially available.
(1) The photosynthetic bacterium capsular rhodobacter A12 strain has a preservation number of CCTCC No: m2018604, the address of the depository is located in Wuhan university in China.
(2) Biocontrol microbial inoculum of photosynthetic bacterium rhodobacter capsulatus A12 strain
The photosynthetic bacteria rhodobacter capsulatus A12 strain is adopted as a raw material, and the strain is prepared by activation, seed culture and production culture, wherein the number of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus A12 strain is 5 × 106The cfu/mL, the specific preparation method comprises the following steps:
s1, activation: culturing the preserved seed of the photosynthetic bacterium rhodobacter capsulatus A12 strain by a double-layer plate culture method until a red single colony appears; the adopted culture medium is a photosynthetic bacteria solid culture medium, and the formula is as follows: peptone: 8g/L, YE: 5.0g/L, glucose: 1.0g/L, storage of trace metal elements: 1.0ml/L, 20g/L agar powder and 7.0 pH value. Preparing a trace metal element liquid storage: accurately weighing EDTA: 2.5g, ZnSO4:6.13g、MgSO4:0.78g、CuSO4:0.25g、CoCl2:0.08g、 FeSO4·7H2O: 7.0g of the extract was dissolved in 1L of steamDistilling the water.
S2, seed culture: inoculating the red single colony cultured in the step S1 into a 120mL serum bottle, and culturing the red single colony in a photosynthetic bacteria liquid culture medium to a logarithmic phase under the conditions of the temperature of 30 ℃, the illumination condition of 3000Lx and the pH value of 7.0 to obtain a bacterial liquid. The formula of the photosynthetic bacteria liquid culture medium is peptone: 8g/L, YE: 5.0g/L, glucose: 1.0g/L, storage of trace metal elements: the 1.0ml/L, pH value was 7.0. Preparing a trace metal element liquid storage: accurately weighing EDTA: 2.5g, ZnSO4:6.13g、MgSO4:0.78g、CuSO4:0.25g、CoCl2:0.08g、FeSO4·7H2O: 7.0g was dissolved in 1L of distilled water.
S3, production culture: inoculating the bacterial liquid obtained after the seed culture in the step S2 into a production bottle, carrying out production culture by using a photosynthetic bacteria strain production culture medium, wherein the photosynthetic bacteria strain production culture medium has the same components as the photosynthetic bacteria liquid culture medium, the inoculation amount of the bacterial liquid is 5% of the total volume of the production culture medium, and the bacterial liquid is cultured to the logarithmic growth phase under the conditions of the temperature of 30 ℃, the illumination condition of 3000Lx and the pH value of 7.0 to obtain the biocontrol bacterial agent of the photosynthetic bacteria capsular red bacteria A12 strain.
(3) Biocontrol agent for photosynthetic bacterium rhodobacter capsulatus A12 strain
The specific preparation method comprises the following steps:
and (3) taking the obtained biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus A12 strain, carrying out fermentation culture for 6d at the temperature of 35 ℃ under the illumination condition of 3000Lx, wherein the components of a fermentation culture medium are the same as those of the photosynthetic bacteria liquid culture medium, centrifuging at 6000rpm for 5min, centrifuging to collect supernatant, filtering the supernatant by using a bacteria filter to obtain supernatant without photosynthetic bacteria rhodobacter capsulatus A12 bacteria, namely the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus A12 strain, and storing at 4 ℃ for later use.
Example 1
The inhibition condition of photosynthetic bacteria strain capsule rhodobacter A12 on pathogenic bacteria causing rice bacterial leaf blight disease is determined by using an inhibition zone method, 0.1ml of pathogenic bacteria is taken and coated on an LB flat plate, a sterilization steel ring is placed in the center, 0.05ml of photosynthetic bacteria liquid is added into the steel ring, the culture is carried out for 7 days at the temperature of 30 ℃, and the existence of the inhibition zone is observed.
Example 2
Under the condition of a greenhouse, 5 × 106The bio-control fungicide of cfu/mL photosynthetic bacterium capsular red bacterium A12 strain is used for soaking rice seeds which are germinated at 30 ℃ until the rice seeds are exposed to white for 1h, the treated rice seeds are sown in pots, seedlings are cultured in a greenhouse under the conditions of 28 ℃ and 10h/d illumination during identification, and the plant height is recorded regularly. 3 representative plants were selected and the plant height and wet weight were determined separately. After the rice seedlings enter the seedling stage, inoculating rice white leaf blight bacteria to the rice seedlings, investigating disease indexes 7 days after inoculation and calculating prevention and control effects.
Comparative example 1
Under the condition of a greenhouse, the rice seeds which are germinated to expose white at the temperature of 30 ℃ are soaked for 1h by adopting a photosynthetic bacteria culture medium, and other steps are the same as the steps in the example 2.
Comparative example 2
The rice seeds germinated to reveal white at 30 ℃ were soaked in water for 1 hour in a greenhouse, and the other steps were the same as in example 2.
In the above example 1, the bacteriostatic circle around the steel ring was determined by the bacteriostatic circle method, which shows that the photosynthetic bacterium strain capsular red bacterium A12 has inhibitory effect on pathogenic bacteria causing bacterial blight disease of rice.
The measurement results of example 2, comparative example 1 and comparative example 2 are shown in table 1.
TABLE 1 measurement results
As can be seen from Table 1, the rice treated with the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus A12 strain has fast growth and strong resistance to bacterial leaf blight of rice. The plant height of the rice is increased by 25.4 percent relative to a clear water control, the fresh weight of a single rice plant is increased by 17.9 percent relative to a clear water control, and the control effect of the bacterial leaf blight of the rice treated by the biocontrol microbial inoculum of the photosynthetic bacterium capsule rhodobacter capsulatus A12 strain reaches 65 percent. The photosynthetic bacteria rhodobacter capsulatus strain has rich biocontrol resources and wide application prospect in preventing and treating the bacterial blight of rice.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> plant protection institute of Hunan province
<120> photosynthetic bacterium capsular red bacterium strain, microbial inoculum medicament, and preparation method and application thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1000
<212>DNA
<213> photosynthetic bacterium Rhodobacter capsulatus A12(Rhodobacter capsulatus A12)
<400>1
gtgatgcggc agctacacat gcagtcgagc gagaccttcg ggtctagcgg cggacgggtg 60
agtaacgcgt gggaacgtgc cctttgctac ggaatagccc cgggaaactg ggagtaatac 120
cgtatgtgcc cttcggggga aagatttatc ggcaaaggat cggcccgcgt tggattaggt 180
agttggtggg gtaatggcct accaagccga cgatccatag ctggtttgag aggatgatca 240
gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg gggaatctta 300
gacaatgggg gaaaccctga tctagccatg ccgcgtgagc gatgaaggcc ttagggttgt 360
aaagctcttt caggtgggaa gataatgacg gtaccaccag aagaagcccc ggctaactcc 420
gtgccagcag ccgcggtaat acggaggggg ctagcgttgt tcggaattac tgggcgtaaa 480
gcgcacgtag gcggatcaga aagtcagagg tgaaatccca gggctcaacc ttggaactgc 540
ctttgaaact cctggtcttg aggtcgagag aggtgagtgg aattccgagt gtagaggtga 600
aattcgtaga tattcggagg aacaccagtg gcgaaggcgg ctcactggct cgatactgac 660
gctgaggtgc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 720
aacgatgaat gccagtcgtc ggcaggcatg cctgtcggtg acacacctaa cggattaagc 780
attccgcctg gggagtacgg tcgcaagatt aaaactcaaa ggaattgacg ggggcccgca 840
caagcggtgg agcatgtggt ttaattcgaa gcaacgcgca gaaccttacc aacccttgac 900
atcgggatcg cggttaccgg agacggtttc cttcagttcg gctggatccc agacaggtgc 960
tgcatggctg tcgtcagctc gtgtcgtgag atgttcggtt 1000