CN110283738B - Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof - Google Patents
Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof Download PDFInfo
- Publication number
- CN110283738B CN110283738B CN201910427672.3A CN201910427672A CN110283738B CN 110283738 B CN110283738 B CN 110283738B CN 201910427672 A CN201910427672 A CN 201910427672A CN 110283738 B CN110283738 B CN 110283738B
- Authority
- CN
- China
- Prior art keywords
- strain
- photosynthetic bacteria
- rhodobacter capsulatus
- photosynthetic
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 119
- 241000894006 Bacteria Species 0.000 title claims abstract description 105
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000003814 drug Substances 0.000 title abstract description 5
- 241000191025 Rhodobacter Species 0.000 title description 5
- 241000191023 Rhodobacter capsulatus Species 0.000 claims abstract description 65
- 230000000443 biocontrol Effects 0.000 claims abstract description 47
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 44
- 235000009566 rice Nutrition 0.000 claims abstract description 44
- 230000001580 bacterial effect Effects 0.000 claims abstract description 26
- 238000000855 fermentation Methods 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims abstract description 7
- 241000209094 Oryza Species 0.000 claims description 46
- 239000001963 growth medium Substances 0.000 claims description 25
- 238000004519 manufacturing process Methods 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 230000012010 growth Effects 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 12
- 238000009630 liquid culture Methods 0.000 claims description 12
- 238000011218 seed culture Methods 0.000 claims description 12
- 241001272684 Xanthomonas campestris pv. oryzae Species 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000012136 culture method Methods 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 4
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 23
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 23
- 239000012681 biocontrol agent Substances 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 244000052616 bacterial pathogen Species 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 244000177578 Bacterium linens Species 0.000 description 5
- 235000012539 Bacterium linens Nutrition 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000001994 activation Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 229910021654 trace metal Inorganic materials 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 231100000674 Phytotoxicity Toxicity 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 241000589652 Xanthomonas oryzae Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052603 melanterite Inorganic materials 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- 239000011686 zinc sulphate Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009654 indole test Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a photosynthetic bacteria capsular red bacterium strain, a microbial inoculum medicament, and a preparation method and application thereof, wherein the photosynthetic bacteria capsular red bacterium strain is photosynthetic bacteria capsular red bacterium A12(Rhodobacter capsulatus A12), which is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604. The culture process of the photosynthetic bacterium capsular red bacterium A12 is simple, the culture time is short, the cost is low, and the photosynthetic bacterium capsular red bacterium strain can be prepared into the biocontrol microbial inoculum for preventing and controlling the bacterial leaf blight of rice and the biocontrol fermentation liquor by a simple, quick and low-cost operation method.
Description
Technical Field
The invention relates to the technical field of biological pesticides, and particularly relates to a photosynthetic bacterium rhodobacter capsulatus strain. In addition, the invention also relates to a microbial inoculum agent comprising the photosynthetic bacterium rhodobacter capsulatus strain, and a preparation method and application thereof.
Background
The bacterial leaf blight of rice is one of important diseases in rice production, is commonly called as plague, is one of main diseases in rice, and is an epidemic disease. The rice bacterial leaf blight is mainly harmful to leaves, causes withering and death of plants, has great influence on the rice yield, and can reduce the yield by about 10 percent generally and 50 to 60 percent or even 90 percent seriously once the rice bacterial leaf blight occurs. The disease is caused by bacteria, and the pathogenic bacteria is xanthomonas oryzae (Xanthomonas oryzae). The primary infection source is mainly diseased grass and diseased grain, and bacteria are spread to the seedling bed through water flow to cause seedling diseases. Early rice seedlings often do not show symptoms after infection and become seedlings with bacteria. Late rice may present symptoms in the three-and four-leaf stage. Flooding in the seedling bed period and in the seedling period, the probability of infection of the seedlings is increased, the more times of flooding and top submerging are increased, the longer the time is, and the higher the bacteria carrying rate of the seedlings is. When the seedlings with the bacterin or the symptomatic seedlings are transplanted to a field, the disease starts to be developed when the disease resistance of the rice plants is reduced at the end stage of tillering, and the rice plants become central disease plants. Then a large amount of bacteria pus is generated at the diseased part of the diseased plant, and the disease is continuously re-infected by water filling and storm propagation, so that the disease is continuously expanded and spread in the field.
The rice bacterial blight mainly overwinter in rice seeds, straws and rice stakes and in nearby soil. And (4) sowing diseased grains, wherein germs can invade through roots and coleoptiles of seedlings. Pathogenic bacteria on diseased straws and rice stakes permeate into water flow when encountering rainwater, seedlings contact with the water with the pathogenic bacteria, and the pathogenic bacteria invade rice bodies from water holes and wounds. The rice straw is used for accelerating germination, covering rice seedlings, binding rice seedlings and the like, which is beneficial to disease spreading. In the rice seedling bed period, because the temperature is low and the bacterial load is less, symptoms can not be seen generally, and the symptoms can not be developed until before and after the booting. Pus discharge on the lesion spots can be re-infected by wind, rain, dew, contact with leaves, etc. Pathogenic bacteria invade through host water pores and wounds to cause diseases. High temperature and raininess, and flooding is most favorable for disease prevalence frequently; improper management of fertilizer and water, biased application of nitrogen fertilizer, deep water irrigation, serial irrigation, flood irrigation or waterlogging of rice fields, are easy to induce disease prevalence; it is more susceptible to diseases.
At present, the prevention and control of rice bacterial blight mainly focuses on agricultural measures such as application of chemical pesticides and breeding of disease-resistant varieties, and the problems of pesticide residue, increase of pest resistance, environmental pollution and the like caused by long-term use of a large amount of chemical pesticides seriously affect the sustainable development of agriculture. And the resistance breeding difficulty is high, the period is long, the corresponding agricultural measures are complex, and the economic investment is large. Biological control is a pest control method which utilizes beneficial organisms or other organisms to eliminate or inhibit harmful organisms, is widely adopted and has good ecological, environmental and social benefits, and is in line with the sustainable development of agriculture due to the advantages of environmental, ecological and human health safety, and is the development trend of current and future plant disease control. In recent years, scholars at home and abroad have made great progress in the fundamental research and application research of photosynthetic bacteria. Many research results prove that the photosynthetic bacteria are rich in various physiological active substances and growth promotion factors, and even extracellular components secreted by the bacteria have stimulation on nitrogen-fixing rhizobia, so that the nitrogen-fixing activity of the bacteria can be obviously improved; and the compound contains substances such as proline, uracil, cytosine and the like, so that the yield of various commercial crops can be increased.
Disclosure of Invention
The invention provides a photosynthetic bacterium capsular rhodobacter strain, a microbial inoculum medicament, and a preparation method and application thereof, and aims to solve the technical problems of high difficulty and high cost in prevention and control of rice bacterial leaf blight in the current rice growth process.
The technical scheme adopted by the invention is as follows:
a photosynthetic bacteria Rhodobacter capsulatus strain is photosynthetic bacteria Rhodobacter capsulatus A12(Rhodobacter capsulatus A12), which is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604.
Further, the photosynthetic bacterium rhodobacter capsulatus strain is cultured on a double-layer solid plate culture medium for 4-8 days to form red round colonies, the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm; the photosynthetic bacteria rhodobacter capsulatus strain presents red color after being cultured in a liquid culture medium for 7 d.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain, which comprises the photosynthetic bacteria capsular red bacterium strain biocontrol microbial inoculum prepared by activating, seed culturing, production culturing and the like.
Further, the bacteria concentration of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain is 5 × 105cfu/mL～5×107cfu/mL。
According to another aspect of the invention, the preparation method of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain comprises the following steps: s1, activation: culturing the preserved strain of the photosynthetic bacterium rhodobacter capsulatus strain by a double-layer plate culture method until a red single colony appears; s2, seed culture: inoculating the red single colony cultured in the step S1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; s3, production culture: inoculating the bacterial liquid obtained in the step S2 according to the inoculation amount of 3% -6% of the total volume of the production medium of the photosynthetic bacteria strain, and culturing to logarithmic phase to obtain the biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacteria strain.
Further, the temperature of the seed culture in the step S2 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria liquid culture medium is 7.0-7.1; in the step S3, the temperature of production and culture is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria strain production culture medium is 7.0-7.1.
According to another aspect of the invention, the biocontrol agent for the photosynthetic bacteria capsular red bacteria strain is prepared by centrifuging the biocontrol agent for the photosynthetic bacteria capsular red bacteria strain and collecting supernatant.
According to another aspect of the invention, there is also provided a preparation method of the biocontrol agent of the above photosynthetic bacterium rhodobacter capsulatus strain, comprising the following steps: fermenting and culturing the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain for 5-7 days, performing centrifugal separation after the culture is finished, collecting supernatant, and filtering by a bacteria filter to obtain the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain.
Further, the conditions of fermentation culture are as follows: the temperature of fermentation culture is 30-35 ℃, and the illumination condition is 3000 Lx-4000 Lx; the conditions of the centrifugal separation were: centrifuging for 5-8 min under the condition of 5000-7000 rpm.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain and a biocontrol agent of the photosynthetic bacteria capsular red bacterium strain, which are applied to the growth promotion of rice or the control of rice bacterial blight.
The invention has the following beneficial effects:
the photosynthetic bacteria capsular red bacterium strain disclosed by the invention has the advantages that the culture process of the photosynthetic bacteria capsular red bacterium A12 is simple, the culture time is short, the cost is low, and the photosynthetic bacteria capsular red bacterium strain can be used for preparing the biocontrol microbial inoculum for controlling the bacterial blight of rice and the biocontrol fermentation liquor by a simple, quick and low-cost operation method.
The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain have the characteristics of environmental friendliness, no toxicity to people and livestock, no phytotoxicity to crops, simple and convenient application and the like, and are not easy to cause drug resistance to diseases and insect pests.
The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain can prevent and control bacterial blight of rice and promote the growth of the rice.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of the biocontrol microbial inoculum of a photosynthetic bacterium rhodobacter capsulatus strain according to a preferred embodiment of the present invention; and
fig. 2 is a schematic diagram of biocontrol agents for the photosynthetic bacteria rhodobacter capsulatus strain of the preferred embodiment of the present invention.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
FIG. 1 is a schematic diagram of the biocontrol microbial inoculum of a photosynthetic bacterium rhodobacter capsulatus strain according to a preferred embodiment of the present invention; fig. 2 is a schematic diagram of biocontrol agents for the photosynthetic bacteria rhodobacter capsulatus strain of the preferred embodiment of the present invention.
The photosynthetic bacteria Rhodobacter capsulatus strain of the present embodiment is a photosynthetic bacteria Rhodobacter capsulatus a12(Rhodobacter capsulatus a12), which is deposited in the chinese typical culture collection center with the collection number of CCTCC No: m2018604. The photosynthetic bacteria capsular red bacterium strain disclosed by the invention has the advantages that the culture process of the photosynthetic bacteria capsular red bacterium A12 is simple, the culture time is short, the cost is low, and the photosynthetic bacteria capsular red bacterium strain can be used for preparing the biocontrol microbial inoculum for controlling the bacterial blight of rice and the biocontrol fermentation liquor by a simple, quick and low-cost operation method.
The photosynthetic bacteria rhodobacter capsulatus strain is photosynthetic bacteria rhodobacter capsulatus A12 strain, is obtained by separating and purifying from soil, is preserved in China center for type culture Collection, and has the preservation number of CCTCC No: m2018604.
The photosynthetic bacterium Rhodobacter capsulatus A12(Rhodobacter capsulatus A12) is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604, the preservation unit address is located in Wuhan university No. 299 in the Wuhan district of Wuhan city, Hubei province, China. The gene sequence is as follows: 1000bp
gtgatgcggcagctacacatgcagtcgagcgagaccttcgggtctagcggcggacgggtgagtaacgcgtgggaacgtgccctttgct acggaatagccccgggaaactgggagtaataccgtatgtgcccttcgggggaaagatttatcggcaaaggatcggcccgcgttggattaggtag ttggtggggtaatggcctaccaagccgacgatccatagctggtttgagaggatgatcagccacactgggactgagacacggcccagactcctac gggaggcagcagtggggaatcttagacaatgggggaaaccctgatctagccatgccgcgtgagcgatgaaggccttagggttgtaaagctctttcaggtgggaagataatgacggtaccaccagaagaagccccggctaactccgtgccagcagccgcggtaatacggagggggctagcgttgttc ggaattactgggcgtaaagcgcacgtaggcggatcagaaagtcagaggtgaaatcccagggctcaaccttggaactgcctttgaaactcctggt cttgaggtcgagagaggtgagtggaattccgagtgtagaggtgaaattcgtagatattcggaggaacaccagtggcgaaggcggctcactggct cgatactgacgctgaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgaatgccagtcgtcggc aggcatgcctgtcggtgacacacctaacggattaagcattccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacgggggcc cgcacaagcggtggagcatgtggtttaattcgaagcaacgcgcagaaccttaccaacccttgacatcgggatcgcggttaccggagacggtttc cttcagttcggctggatcccagacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttcggtt
In the embodiment, the photosynthetic bacterium rhodobacter capsulatus strain is cultured on a double-layer solid plate culture medium for 4-8 days to form red round colonies, the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm; the photosynthetic bacteria rhodobacter capsulatus strain is cultured in a liquid culture medium for 78d to be red. The photosynthetic bacterium Rhodobacter capsulatus A12 strain is a gram-negative bacterium and is identified as Rhodobacter capsulatus A12 through physiological and biochemical identification and molecular biological identification. The main biological characteristics are as follows: culturing for 4-8 days on a double-layer solid plate culture medium to form red round colonies, wherein the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm. The color of the solution was red when cultured in liquid medium for 7 d. Good growth under anaerobic and micro-aerobic conditions. The physiochemical characteristics are V-P reaction and methyl red reaction negative, H2The S reaction, the gelatin liquefaction, the urease test and the indole test are all positive, the optimal growth temperature is 30-35 ℃, and the pH value is 7.
Antagonistic microorganisms reported for the biological control of rice bacterial blight are mainly fungi, bacteria, slime mold, marine microorganisms, wherein the biocontrol bacteria comprise bacillus, pseudomonas and the like. The mechanism of inhibiting the bacterial blight of rice by the photosynthetic bacterium capsular red bacterium A12 is mainly to inhibit the growth of pathogenic bacteria by secreting one or more antibacterial substances such as bacteriocins, proteins, lipopeptides, polypeptides, phenols and the like, thereby preventing the occurrence of plant diseases. In addition, the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus A12 strain induces the plants to generate disease resistance potential, thereby enhancing the disease resistance of the plants. And the photosynthetic bacteria rhodobacter capsulatus strain has some active substances, can inactivate the pathogenicity of pathogens, promote the proliferation of beneficial microorganisms such as actinomycetes and the like, and inhibit the growth of the pathogens, thereby effectively inhibiting the occurrence and spread of certain plant diseases. Therefore, the photosynthetic bacterium capsular red bacterium A12 strain has rich biocontrol resources and wide application prospect in preventing and treating the bacterial blight of rice.
As shown in fig. 1, according to another aspect of the present invention, there is also provided a biocontrol microbial inoculum of a photosynthetic bacteria capsular red bacterium strain, which comprises the photosynthetic bacteria capsular red bacterium strain biocontrol microbial inoculum prepared by activating, seed culturing, production culturing and the like of the photosynthetic bacteria capsular red bacterium strain. The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain have the characteristics of environmental friendliness, no toxicity to people and livestock, no phytotoxicity to crops, simple and convenient application and the like, and are not easy to cause drug resistance to diseases and insect pests. The biocontrol microbial inoculum and the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus strain can prevent and control bacterial blight of rice and promote the growth of the rice.
In this example, the concentration of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain is 5 × 105 cfu/mL-5 × 107cfu/mL。
According to another aspect of the invention, the preparation method of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain comprises the following steps: s1, activation: culturing the preserved strain of the photosynthetic bacterium rhodobacter capsulatus strain by a double-layer plate culture method until a red single colony appears; s2, seed culture: inoculating the red single colony cultured in the step S1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; s3, production culture: inoculating the bacterial liquid obtained in the step S2 according to the inoculation amount of 3% -6% of the total volume of the production medium of the photosynthetic bacteria strain, and culturing to logarithmic phase to obtain the biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacteria strain.
In this embodiment, the optimal temperature for seed culture in step S2 is 30 ℃ to 35 ℃, the illumination conditions are 2500Lx to 4000Lx, and the optimal pH of the photosynthetic bacteria liquid culture medium is 7.0 to 7.1. The temperature of the production culture in the step S3 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the optimum pH value of the photosynthetic bacteria strain production culture medium is 7.0-7.1.
As shown in figure 1, according to another aspect of the invention, a biocontrol agent for a photosynthetic bacteria capsular red bacterium strain is also provided, and is prepared by centrifuging the biocontrol agent for the photosynthetic bacteria capsular red bacterium strain and collecting supernatant. According to another aspect of the invention, there is also provided a preparation method of the biocontrol agent of the above photosynthetic bacterium rhodobacter capsulatus strain, comprising the following steps: fermenting and culturing the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain for 5-7 days, performing centrifugal separation after the culture is finished, collecting supernatant, and filtering by a bacteria filter to obtain the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain.
In this example, the conditions of the fermentation culture were: the temperature of fermentation culture is 30-35 ℃, and the illumination condition is 3000 Lx-4000 Lx. The conditions of the centrifugal separation were: centrifuging for 5-8 min under the condition of 5000-7000 rpm.
According to another aspect of the invention, the invention also provides a biocontrol microbial inoculum of the photosynthetic bacteria capsular red bacterium strain and a biocontrol agent of the photosynthetic bacteria capsular red bacterium strain, which are applied to the growth promotion of rice or the control of rice bacterial blight.
Examples
In the following examples, each chemical reagent is commercially available.
(1) The photosynthetic bacterium capsular rhodobacter A12 strain has a preservation number of CCTCC No: m2018604, the address of the depository is located in Wuhan university in China.
(2) Biocontrol microbial inoculum of photosynthetic bacterium rhodobacter capsulatus A12 strain
The photosynthetic bacteria rhodobacter capsulatus A12 strain is adopted as a raw material, and the strain is prepared by activation, seed culture and production culture, wherein the number of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus A12 strain is 5 × 106The cfu/mL, the specific preparation method comprises the following steps:
s1, activation: culturing the preserved seed of the photosynthetic bacterium rhodobacter capsulatus A12 strain by a double-layer plate culture method until a red single colony appears; the adopted culture medium is a photosynthetic bacteria solid culture medium, and the formula is as follows: peptone: 8g/L, YE: 5.0g/L, glucose: 1.0g/L, storage of trace metal elements: 1.0ml/L, 20g/L agar powder and 7.0 pH value. Preparing a trace metal element liquid storage: accurately weighing EDTA: 2.5g, ZnSO4：6.13g、MgSO4：0.78g、CuSO4：0.25g、CoCl2：0.08g、 FeSO4·7H2O: 7.0g of the extract was dissolved in 1L of steamDistilling the water.
S2, seed culture: inoculating the red single colony cultured in the step S1 into a 120mL serum bottle, and culturing the red single colony in a photosynthetic bacteria liquid culture medium to a logarithmic phase under the conditions of the temperature of 30 ℃, the illumination condition of 3000Lx and the pH value of 7.0 to obtain a bacterial liquid. The formula of the photosynthetic bacteria liquid culture medium is peptone: 8g/L, YE: 5.0g/L, glucose: 1.0g/L, storage of trace metal elements: the 1.0ml/L, pH value was 7.0. Preparing a trace metal element liquid storage: accurately weighing EDTA: 2.5g, ZnSO4：6.13g、MgSO4：0.78g、CuSO4：0.25g、CoCl2：0.08g、FeSO4·7H2O: 7.0g was dissolved in 1L of distilled water.
S3, production culture: inoculating the bacterial liquid obtained after the seed culture in the step S2 into a production bottle, carrying out production culture by using a photosynthetic bacteria strain production culture medium, wherein the photosynthetic bacteria strain production culture medium has the same components as the photosynthetic bacteria liquid culture medium, the inoculation amount of the bacterial liquid is 5% of the total volume of the production culture medium, and the bacterial liquid is cultured to the logarithmic growth phase under the conditions of the temperature of 30 ℃, the illumination condition of 3000Lx and the pH value of 7.0 to obtain the biocontrol bacterial agent of the photosynthetic bacteria capsular red bacteria A12 strain.
(3) Biocontrol agent for photosynthetic bacterium rhodobacter capsulatus A12 strain
The specific preparation method comprises the following steps:
and (3) taking the obtained biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus A12 strain, carrying out fermentation culture for 6d at the temperature of 35 ℃ under the illumination condition of 3000Lx, wherein the components of a fermentation culture medium are the same as those of the photosynthetic bacteria liquid culture medium, centrifuging at 6000rpm for 5min, centrifuging to collect supernatant, filtering the supernatant by using a bacteria filter to obtain supernatant without photosynthetic bacteria rhodobacter capsulatus A12 bacteria, namely the biocontrol agent of the photosynthetic bacteria rhodobacter capsulatus A12 strain, and storing at 4 ℃ for later use.
Example 1
The inhibition condition of photosynthetic bacteria strain capsule rhodobacter A12 on pathogenic bacteria causing rice bacterial leaf blight disease is determined by using an inhibition zone method, 0.1ml of pathogenic bacteria is taken and coated on an LB flat plate, a sterilization steel ring is placed in the center, 0.05ml of photosynthetic bacteria liquid is added into the steel ring, the culture is carried out for 7 days at the temperature of 30 ℃, and the existence of the inhibition zone is observed.
Example 2
Under the condition of a greenhouse, 5 × 106The bio-control fungicide of cfu/mL photosynthetic bacterium capsular red bacterium A12 strain is used for soaking rice seeds which are germinated at 30 ℃ until the rice seeds are exposed to white for 1h, the treated rice seeds are sown in pots, seedlings are cultured in a greenhouse under the conditions of 28 ℃ and 10h/d illumination during identification, and the plant height is recorded regularly. 3 representative plants were selected and the plant height and wet weight were determined separately. After the rice seedlings enter the seedling stage, inoculating rice white leaf blight bacteria to the rice seedlings, investigating disease indexes 7 days after inoculation and calculating prevention and control effects.
Comparative example 1
Under the condition of a greenhouse, the rice seeds which are germinated to expose white at the temperature of 30 ℃ are soaked for 1h by adopting a photosynthetic bacteria culture medium, and other steps are the same as the steps in the example 2.
Comparative example 2
The rice seeds germinated to reveal white at 30 ℃ were soaked in water for 1 hour in a greenhouse, and the other steps were the same as in example 2.
In the above example 1, the bacteriostatic circle around the steel ring was determined by the bacteriostatic circle method, which shows that the photosynthetic bacterium strain capsular red bacterium A12 has inhibitory effect on pathogenic bacteria causing bacterial blight disease of rice.
The measurement results of example 2, comparative example 1 and comparative example 2 are shown in table 1.
TABLE 1 measurement results
As can be seen from Table 1, the rice treated with the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus A12 strain has fast growth and strong resistance to bacterial leaf blight of rice. The plant height of the rice is increased by 25.4 percent relative to a clear water control, the fresh weight of a single rice plant is increased by 17.9 percent relative to a clear water control, and the control effect of the bacterial leaf blight of the rice treated by the biocontrol microbial inoculum of the photosynthetic bacterium capsule rhodobacter capsulatus A12 strain reaches 65 percent. The photosynthetic bacteria rhodobacter capsulatus strain has rich biocontrol resources and wide application prospect in preventing and treating the bacterial blight of rice.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> plant protection institute of Hunan province
<120> photosynthetic bacterium capsular red bacterium strain, microbial inoculum medicament, and preparation method and application thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1000
<212>DNA
<213> photosynthetic bacterium Rhodobacter capsulatus A12(Rhodobacter capsulatus A12)
<400>1
gtgatgcggc agctacacat gcagtcgagc gagaccttcg ggtctagcgg cggacgggtg 60
agtaacgcgt gggaacgtgc cctttgctac ggaatagccc cgggaaactg ggagtaatac 120
cgtatgtgcc cttcggggga aagatttatc ggcaaaggat cggcccgcgt tggattaggt 180
agttggtggg gtaatggcct accaagccga cgatccatag ctggtttgag aggatgatca 240
gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg gggaatctta 300
gacaatgggg gaaaccctga tctagccatg ccgcgtgagc gatgaaggcc ttagggttgt 360
aaagctcttt caggtgggaa gataatgacg gtaccaccag aagaagcccc ggctaactcc 420
gtgccagcag ccgcggtaat acggaggggg ctagcgttgt tcggaattac tgggcgtaaa 480
gcgcacgtag gcggatcaga aagtcagagg tgaaatccca gggctcaacc ttggaactgc 540
ctttgaaact cctggtcttg aggtcgagag aggtgagtgg aattccgagt gtagaggtga 600
aattcgtaga tattcggagg aacaccagtg gcgaaggcgg ctcactggct cgatactgac 660
gctgaggtgc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 720
aacgatgaat gccagtcgtc ggcaggcatg cctgtcggtg acacacctaa cggattaagc 780
attccgcctg gggagtacgg tcgcaagatt aaaactcaaa ggaattgacg ggggcccgca 840
caagcggtgg agcatgtggt ttaattcgaa gcaacgcgca gaaccttacc aacccttgac 900
atcgggatcg cggttaccgg agacggtttc cttcagttcg gctggatccc agacaggtgc 960
tgcatggctg tcgtcagctc gtgtcgtgag atgttcggtt 1000
Claims (6)
1. A photosynthetic bacteria Rhodobacter capsulatus strain A12 is characterized by being preserved in China center for type culture Collection with the preservation number of CCTCC No: m2018604.
2. A biocontrol microbial inoculum of a photosynthetic bacteria rhodobacter capsulatus strain A12 is characterized in that the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain A12 is prepared by activating, seed culturing and fermenting the photosynthetic bacteria rhodobacter capsulatus strain A12 of claim 1.
3. The biocontrol microbial inoculum of photosynthetic bacteria rhodobacter capsulatus strain A12 of claim 2,
the bacteria concentration of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain is 5 × 105cfu/mL～5×107cfu/mL。
4. The preparation method of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain A12 as claimed in claim 2 or 3, characterized by comprising the following steps:
s1, activation: culturing the preserved strain of the photosynthetic bacterium rhodobacter capsulatus strain by a double-layer plate culture method until a red single colony appears;
s2, seed culture: inoculating the red single colony cultured in the step S1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid;
s3, fermentation: inoculating the bacterial liquid obtained in the step S2 according to the inoculation amount of 3-6% of the total volume of the fermentation medium of the photosynthetic bacteria strain, and culturing to logarithmic phase to obtain the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain A12.
5. The preparation method of the biocontrol microbial inoculum of the photosynthetic bacteria rhodobacter capsulatus strain A12 as claimed in claim 4,
the temperature of the seed culture in the step S2 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria liquid culture medium is 7.0-7.1;
the temperature of the production culture in the step S3 is 30-35 ℃, the illumination condition is 2500 Lx-4000 Lx, and the pH value of the photosynthetic bacteria strain production culture medium is 7.0-7.1.
6. The application of the biocontrol microbial inoculum of the photosynthetic bacterium rhodobacter capsulatus strain A12 as claimed in claim 2 in promoting the growth of rice or preventing and treating bacterial blight of rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910427672.3A CN110283738B (en) | 2019-05-22 | 2019-05-22 | Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910427672.3A CN110283738B (en) | 2019-05-22 | 2019-05-22 | Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110283738A CN110283738A (en) | 2019-09-27 |
| CN110283738B true CN110283738B (en) | 2020-10-16 |
Family
ID=68002221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910427672.3A Active CN110283738B (en) | 2019-05-22 | 2019-05-22 | Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110283738B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753408A (en) * | 2020-12-31 | 2021-05-07 | 哈尔滨奥农生物科技发展有限公司 | Application and method of photosynthetic bacteria for preventing and treating rice straw returning reduced acidosis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724585A (en) * | 2009-11-19 | 2010-06-09 | 湖南省植物保护研究所 | Rhodobacter strain and application thereof |
| CN105164260A (en) * | 2013-03-13 | 2015-12-16 | 自动显示生物技术有限公司 | Improved surface display of functional proteins in a broad range of gram negative bacteria |
| CN108192851A (en) * | 2018-03-13 | 2018-06-22 | 长沙艾格里生物肥料技术开发有限公司 | Dongfanghong bacteria strain, Dongfanghong bacteria agent and its application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100835817B1 (en) * | 2007-05-02 | 2008-06-09 | 박형두 | Soil conditioner manufucturing method and it's utilize method ustural minerals and microorganism |
-
2019
- 2019-05-22 CN CN201910427672.3A patent/CN110283738B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724585A (en) * | 2009-11-19 | 2010-06-09 | 湖南省植物保护研究所 | Rhodobacter strain and application thereof |
| CN105164260A (en) * | 2013-03-13 | 2015-12-16 | 自动显示生物技术有限公司 | Improved surface display of functional proteins in a broad range of gram negative bacteria |
| CN108192851A (en) * | 2018-03-13 | 2018-06-22 | 长沙艾格里生物肥料技术开发有限公司 | Dongfanghong bacteria strain, Dongfanghong bacteria agent and its application |
Non-Patent Citations (2)
| Title |
|---|
| Bacterial leaf blight resistance in rice: a review of conventional;S. C. Chukwu等;《Molecular Biology Reports》;20190109;第1519–1532页 * |
| 水稻白叶枯病无毒因子Ax21成熟机理初探;郑勇;《中国优秀硕士学位论文全文数据库》;20130315;摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110283738A (en) | 2019-09-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105886428B (en) | One plant of Streptomycesalbidoflhaving and its application in microbial manure | |
| CN101525587B (en) | Streptomyces strain and application thereof | |
| CN109234197B (en) | Pseudomonas aeruginosa capable of preventing and treating tobacco black shank and black root rot simultaneously and having growth promoting effect and application thereof | |
| CN111690578B (en) | Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof | |
| CN107287130A (en) | A kind of Streptomycesalbidoflhaving bacterial strain and its application in agricultural chemicals | |
| CN109182219A (en) | One plant of Mo Haiwei bacillus for promoting the growth of Henry David Thoreau grass and its application | |
| CN111705020A (en) | Pseudomonas chlororaphis aurantiacus subspecies and preparation and application of microbial agent thereof | |
| CN104152372B (en) | The biocontrol bacterial strain G68 of controlling plant diseases and its bacterial preparation process and application | |
| CN110283738B (en) | Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof | |
| CN107058154B (en) | Rhodotorula orientalis strain, biocontrol microbial inoculum, biocontrol fermentation liquor as well as preparation method and application of biocontrol fermentation liquor | |
| CN111808778A (en) | Bacillus wegener for preventing and treating verticillium wilt and culture method thereof, microbial inoculum and preparation method and application thereof | |
| CN109055265B (en) | Biocontrol bacterium and application thereof in prevention and control of gummy stem blight of crops | |
| CN115505541B (en) | Biocontrol bacterium Burkholderia pyrrocinia suitable for wheat fungal diseases and application thereof | |
| CN116790417A (en) | Pseudomonas capable of inhibiting pathogenic bacteria of various plants and having growth promoting effect on various crops, screening method and application | |
| CN115873770A (en) | Bacillus belgii and application thereof in prevention and treatment of tomato diseases | |
| CN114456973B (en) | Streptomyces rochei in tobacco and application thereof in prevention and control of tobacco diseases | |
| CN110205263B (en) | Photosynthetic bacterium rhodobacter sphaeroides strain, microbial inoculum medicament, preparation method and application thereof | |
| CN110205262B (en) | Rhodobacter sphaeroides A2 strain, biocontrol microbial inoculum and preparation method and application thereof | |
| CN110396477B (en) | Trichoderma mutant strain suitable for being compounded with hymexazol to prevent and treat melon blight, fungicide and application thereof | |
| CN114774288B (en) | Trichoderma harzianum and application thereof, and microbial agent for resisting anthracnose and promoting growth and development of strawberries | |
| CN113913303B (en) | Trichoderma pseudokoningii, root soaking solution and application | |
| CN112877222B (en) | Strain for antagonizing sclerotinia rot of asarum and application thereof | |
| CN112920963B (en) | Biocontrol strain and biocontrol microbial inoculum for preventing and treating cotton wilt and preparation method and application thereof | |
| CN111334453B (en) | Biocontrol bacterium and application thereof in prevention and control of sheath blight of crops | |
| CN116286499A (en) | Efficient antagonistic bacteria agent for verticillium wilt and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20190927 Assignee: CHANGSHA AGRI BIOLOGY TECHNOLOGY Co.,Ltd. Assignor: HUNAN PLANT PROTECTION INSTITUTE Contract record no.: X2020430000013 Denomination of invention: Photosynthetic bacteria rhodobacteria capsulata strains, fungicides and their preparation methods and Applications Granted publication date: 20201016 License type: Common License Record date: 20201221 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: CHANGSHA AGRI BIOLOGY TECHNOLOGY CO.,LTD. Assignor: HUNAN PLANT PROTECTION INSTITUTE Contract record no.: X2020430000013 Date of cancellation: 20231227 |
|
| EC01 | Cancellation of recordation of patent licensing contract |