CN110265088A - A kind of method that DNA is converted into pattern - Google Patents
A kind of method that DNA is converted into pattern Download PDFInfo
- Publication number
- CN110265088A CN110265088A CN201910513224.5A CN201910513224A CN110265088A CN 110265088 A CN110265088 A CN 110265088A CN 201910513224 A CN201910513224 A CN 201910513224A CN 110265088 A CN110265088 A CN 110265088A
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- Prior art keywords
- dna
- converted
- str locus
- pattern
- amplification kit
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Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000012197 amplification kit Methods 0.000 claims abstract description 15
- 230000006870 function Effects 0.000 claims abstract description 4
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 3
- 229910003460 diamond Inorganic materials 0.000 claims description 7
- 239000010432 diamond Substances 0.000 claims description 7
- 241001465754 Metazoa Species 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 241000218636 Thuja Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- VDGJOQCBCPGFFD-UHFFFAOYSA-N oxygen(2-) silicon(4+) titanium(4+) Chemical compound [Si+4].[O-2].[O-2].[Ti+4] VDGJOQCBCPGFFD-UHFFFAOYSA-N 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/20—Sequence assembly
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Analytical Chemistry (AREA)
- Medical Informatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Theoretical Computer Science (AREA)
- Evolutionary Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Data Mining & Analysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of methods that DNA is converted into pattern, comprising the following steps: step 1: extracting the core DNA for the sample that can measure DNA that user provides；Step 2: using amplification kit, expand specific str locus site；Step 3: fluorescence detection being carried out to amplified production, obtains the genotype or sequence signature of its str locus seat；Step 4: str locus seat genotype is converted into base sequence in conjunction with the number of repetition of core sequence by the STR core sequence for the str locus seat inquired, which can guarantee the uniqueness with individual；Step 5: in conjunction with scheme of colour, determining a kind of color matching respectively for tetra- kinds of bases of ATCG；Step 6: computer language of choosing any one kind of them draws out the figure of user preferences according to base sum using drawing function or sentence, and forms a face.DNA is converted unique pattern by the method that a kind of DNA provided by the invention is converted into pattern, and pattern can be applied to various occasions, meet individual demand.
Description
Technical field
The invention belongs to the applications of biological field, are related to a kind of method that DNA is converted into pattern.
Background technique
The many people of modern society are pursuing personalized, personalization, are just non-general popular things as its name suggests.
Increase needs that are unique, atypical, possessing oneself speciality, a kind of original in style, strange and new saying on the basis of popular.
Make a kind of distinguished effect.
It for personalization, is being best embodied in clothes dress ornament, especially on the pattern of clothes dress ornament, for the clothes of volume production
Pattern has identical certainly, this and personalization are disagreed, and for the clothes of customization, pattern is likely to the figure customized with others
Case is identical.
DNA(DNA) be human body inner cell substance.Each nucleus has 46 chromosomes, due to human body
There are about 3,000,000,000 base-pairs to constitute whole chromosome systems, and exchange before reproduction cell is formed and combination be it is random,
So there is the composition sequence of identical 3,000,000,000 nucleotide without any two people in the world, if using DNA as figure
Case is printed on dress ornament or other aspects, has fully met personalization, unique.
Summary of the invention
1, technical problem to be solved:
Many people of modern society pursue personalization, especially pattern, but inevitably will appear repetition.
2, technical solution:
In order to solve problem above, the present invention provides a kind of methods that DNA is converted into pattern, comprising the following steps: step 1:
Extract the core DNA for the sample that can measure DNA that user provides；Step 2: using amplification kit, expand specific str locus
Site enters next step, or then enters step 5 using amplification kit sequencing；Step 3: amplified production being carried out glimmering
Light detection obtains the genotype or sequence signature of its str locus seat；Step 4: the STR core sequence for the str locus seat inquired
Column, in conjunction with the number of repetition of core sequence, are converted into base sequence for str locus seat genotype, which can guarantee have
The uniqueness of individual；Step 5: in conjunction with scheme of colour, determining a kind of color matching respectively for tetra- kinds of bases of ATCG；Step 6: choosing any one kind of them
Computer language draws out the figure of user preferences according to the sum of base obtained in step 4 using drawing function or sentence,
And a face is formed, which can directly generate or be cut into various shape by user demand.
The sample that can measure DNA that user provides is people's or animal.
The method for determining color matching is that close, phase secondary color is chosen in colour wheel figure.
The figure is figure that can be seamless spliced.
The figure is triangle or rectangular or diamond shape or hexagon or diamond or rectangle or other seamless can spell
The figure connect.
In step 2, the amplification kit that the amplification kit is and is commercialized.
The amplification kit is GLOBALFILER amplification kit.
3, the utility model has the advantages that
The method that a kind of DNA provided by the invention is converted into pattern converts first without two pattern for DNA, and pattern can answer
For various occasions, individual demand is met.
Specific embodiment
The present invention will be described in detail below.
The present invention provides a kind of methods that DNA is converted into pattern, including following 6 steps, step 1: extract people or
The core DNA of animal；Step 2: using amplification kit, expand specific str locus site and enter next step, or use
Amplification kit sequencing then enters step 5；Step 3: fluorescence detection being carried out to amplified production, obtains the base of its str locus seat
Because of features such as type or sequences；Step 4: the STR core sequence for the str locus seat inquired, in conjunction with the repetition time of core sequence
Number, is converted into base sequence for str locus seat genotype, which can guarantee the uniqueness with individual；Step 5, which combines, matches
Color scheme determines a kind of color matching for tetra- kinds of bases of ATCG respectively, and choosing method preferably chooses close, phase secondary color in colour wheel figure；Step
Rapid 6: computer language of choosing any one kind of them draws out use according to the sum of base obtained in step 4 using drawing function or sentence
Triangle, rectangular, diamond shape, the hexagon etc. of family hobby can be seamless spliced figure, and form a face, which can be by user
Demand directly generates or is cut into various shape.
It is operated according to above-mentioned steps, can be with individual by str locus seat Feature Conversion according to the DNA of human or animal
The pattern of uniqueness.
Embodiment
By taking Sun as an example, its saliva sample is taken, the nucleus DNA of the sample is extracted with titanium dioxide silicon process.
The DNA extracted is taken, with GLOBALFILER amplification kit, composite amplification is carried out on amplification instrument.
Take amplified production appropriate, the electrophoresis on genetic analyzer, analysis obtain D8S1179, D21S11 on 22 pairs of chromosomes,
D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、TPOX、D18S51、
The parting number in the str locus such as D5S818, FGA, D12S391, D1S1656, D2S441, D10S1248, D22S1045, SE33 site
According to.
With PHP language, the arranging situation of the core repeat sequence according to above-mentioned STR bit point, according to personal preference to four seed nucleus
Thuja acid is matched colors, and the patterns such as circle, triangle, diamond, diamond shape, rectangle are generated.
Pattern can print in the personal belongings such as clothing, mobile phone shell.
Although the present invention has been described by way of example and in terms of the preferred embodiments, they be not it is for the purpose of limiting the invention, it is any ripe
This those skilled in the art is practised, without departing from the spirit and scope of the invention, can make various changes or retouch from working as, therefore guarantor of the invention
Shield range should be subject to what claims hereof protection scope was defined.
Claims (7)
1. a kind of method that DNA is converted into pattern, comprising the following steps: step 1: that extracts user's offer can measure DNA's
The core DNA of object；Step 2: using amplification kit, expand specific str locus site and enter next step, or use
Amplification kit sequencing then enters step 5；Step 3: fluorescence detection being carried out to amplified production, obtains the base of its str locus seat
Because of type or sequence signature；Step 4: the STR core sequence for the str locus seat inquired, in conjunction with the number of repetition of core sequence,
Str locus seat genotype is converted into base sequence, which can guarantee the uniqueness with individual；Step 5: in conjunction with color matching
Scheme determines a kind of color matching for tetra- kinds of bases of ATCG respectively；Step 6: computer language of choosing any one kind of them uses drawing function or language
Sentence draws out the figure of user preferences according to the sum of base obtained in step 4, and forms a face, which can be needed by user
It asks, directly generates or be cut into various shape.
2. the method as described in claim 1, it is characterised in that: user provide the object that can measure DNA be people's or
Animal.
3. the method as described in claim 1, it is characterised in that: in steps of 5, the method for determining color matching is to select in colour wheel figure
Take close, phase secondary color.
4. the method as described in claim 1, it is characterised in that: the figure is figure that can be seamless spliced.
5. method as claimed in claim 4, it is characterised in that: the figure is triangle or rectangular or diamond shape or hexagon
Or diamond or rectangle or other figures that can be seamless spliced.
6. the method as described in claim 1-5 any claim, it is characterised in that: in step 2, the amplifing reagent
Box is the amplification kit of commercialization.
7. method as claimed in claim 6, it is characterised in that: in step 2, the amplification kit is
GLOBALFILER amplification kit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910513224.5A CN110265088A (en) | 2019-06-14 | 2019-06-14 | A kind of method that DNA is converted into pattern |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910513224.5A CN110265088A (en) | 2019-06-14 | 2019-06-14 | A kind of method that DNA is converted into pattern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110265088A true CN110265088A (en) | 2019-09-20 |
Family
ID=67918156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910513224.5A Pending CN110265088A (en) | 2019-06-14 | 2019-06-14 | A kind of method that DNA is converted into pattern |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703595A (en) * | 2012-06-13 | 2012-10-03 | 东南大学 | STR (short tandem repeat) sequence high-throughput detection method with base selective controllable extension and detection reagent thereof |
| WO2016049877A1 (en) * | 2014-09-30 | 2016-04-07 | 深圳华大基因股份有限公司 | Detecting methods and systems based on str typing technology for non-invasive prenatal testing |
| CN106399563A (en) * | 2016-11-16 | 2017-02-15 | 贵州申科生物科技有限公司 | Multi-locus STR analysis method |
| CN108660248A (en) * | 2018-05-25 | 2018-10-16 | 浙江省农业科学院 | A kind of method of the long pod cowpea variety of molecular marking supplementary breeding |
-
2019
- 2019-06-14 CN CN201910513224.5A patent/CN110265088A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703595A (en) * | 2012-06-13 | 2012-10-03 | 东南大学 | STR (short tandem repeat) sequence high-throughput detection method with base selective controllable extension and detection reagent thereof |
| WO2016049877A1 (en) * | 2014-09-30 | 2016-04-07 | 深圳华大基因股份有限公司 | Detecting methods and systems based on str typing technology for non-invasive prenatal testing |
| CN106399563A (en) * | 2016-11-16 | 2017-02-15 | 贵州申科生物科技有限公司 | Multi-locus STR analysis method |
| CN108660248A (en) * | 2018-05-25 | 2018-10-16 | 浙江省农业科学院 | A kind of method of the long pod cowpea variety of molecular marking supplementary breeding |
Non-Patent Citations (1)
| Title |
|---|
| 热衷组培的二货潜: ""计算基因序列中ATCG的含量以及碱基motif的绘制", 《简书》 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190920 |
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| RJ01 | Rejection of invention patent application after publication |