CN110255703B - Rapid culture method of aerobic ammonia oxidation granular sludge - Google Patents
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Abstract
A method for quickly culturing aerobic ammonia oxidation granular sludge comprises the steps of inoculating aerobic granular sludge mainly containing heterotrophic bacteria into a sequencing batch reactor to serve as a carrier, gradually reducing the COD value of inlet water to zero concentration of organic matters to domesticate the aerobic ammonia oxidation granular sludge, and adding a high-efficiency nitrobacteria concentrated solution in the domestication process to promote the quick enrichment of autotrophic bacteria in the granular sludge and quickly culture the aerobic ammonia oxidation granular sludge. The method makes full use of the biological carrier effect of the heterotrophic granular sludge, overcomes the defect of low proliferation rate of nitrifying bacteria by adding exogenous aerobic ammonia oxidizing bacteria, realizes the rapid enrichment of the aerobic ammonia oxidizing bacteria in the heterotrophic granular sludge, fully exerts the advantages of good settling property, high toxicity resistance and the like of AGS, and provides technical support for harmless treatment of wastewater. The method can effectively reduce the impact of the sharp change of the operation condition on the stability of the inoculated AGS, and realize the replacement of heterotrophic bacteria in granules by aerobic ammonia oxidizing bacteria in a short time.
Description
Technical Field
The invention relates to the technical field of biological sewage treatment.
Background
Aerobic Granular Sludge (AGS) is a granular biopolymer formed by spontaneous coagulation and proliferation of microorganisms in a specific environment, and has the advantages of compact structure, good settling property, high toxicity resistance, synchronous nitrogen and phosphorus removal and the like. Practice proves that: compared with the traditional activated sludge process, the AGS technology can greatly reduce the construction investment and the operation cost and has good removal effect on toxic and harmful wastewater. AGS is classified into heterotrophic granular sludge and autotrophic granular sludge according to the kind of carbon source used. The autotrophic nitrification granular sludge is favored due to good nitrification performance and stability, and provides a new idea for treating the rare earth mine wastewater in the Gannan province. Unfortunately, the self-aggregation between nitrifying bacteria is very slow due to their very low proliferation rate. Research shows that the formation time of autotrophic nitrification granular sludge is usually more than two months, even up to several years, by using the activated sludge as the inoculation sludge, the development and popularization of the technology are greatly limited. At present, a plurality of cases have appeared about accelerating the aerobic granulation process of the AGS by inoculation and storing and rapidly recovering the AGS, which shows that the AGS is a good biological carrier and can be used as a medium for the growth and enrichment of specific functional bacteria. In addition, heterotrophic granular sludge has been engineered for easier access than autotrophic nitrifying granular sludge, and it has been shown that seeding heterotrophic granular sludge accelerates the formation of autotrophic nitrifying granular sludge. The defects that the heterotrophic granular sludge is directly used for acclimatizing the autotrophic nitrification granular sludge by using the inorganic wastewater, the phenomena of granule disintegration and functional bacteria loss are easy to occur, so that the operation condition is strictly controlled when the strategy is applied, and whether the strategy is applied or not still needs to be checked.
The prior published documents mainly comprise a preparation and restoration method of aerobic nitrification granular sludge with publication number CN101386448, a long-term preservation and activity recovery method of aerobic nitrification granular sludge with publication number CN103880168A, a method for culturing nitrification granular sludge by using anaerobic granular sludge with publication number CN1821122, a culture method of denitrification granular sludge with publication number CN102040281A, a method for dual inhibition culture of short-range nitrification granular sludge by combining FA and FNA with publication number CN102173504A, a culture method for treating domestic sewage nitrosation aerobic granular sludge with publication number CN101531983, a heterotrophic nitrification aerobic granular sludge with publication number CN101306870 and a culture method and application thereof, a culture method of aerobic nitrosation granular sludge with publication number CN 16999217, a method for realizing autotrophic denitrification of municipal sewage by using floc sludge and granular sludge with publication number CN104529056A, and a culture method of normal temperature nitrosation granular sludge for treating municipal sewage with publication number CN103922466A, the continuous flow biological denitrification method and device based on the granular sludge with the publication number of CN103663725A do not have the technical scheme of adding exogenous nitrobacteria to promote the quick formation of autotrophic nitrification granular sludge.
Disclosure of Invention
The invention aims to solve the problems that the autotrophic nitrification granular sludge is too long in culture time and short-range nitrification is difficult to realize quickly and the like, and provides a quick culture method of aerobic ammonia oxidation granular sludge.
The purpose of the invention is realized by the following technical scheme:
a method for quickly culturing aerobic ammonia oxidation granular sludge includes inoculating aerobic granular sludge mainly containing heterotrophic bacteria to a sequencing batch reactor as a carrier, gradually reducing the COD value of inlet water to zero concentration of organic matters to acclimate the aerobic ammonia oxidation granular sludge, and adding high-efficiency nitrobacteria concentrated solution during the acclimation process to promote the autotrophic bacteria to be quickly enriched in the granular sludge to quickly culture the aerobic ammonia oxidation granular sludge.
The inoculated aerobic granular sludge has autotrophic bacteria ratio less than or equal to 10 percent, sedimentation speed more than or equal to 15m/h, and MLSS more than or equal to 3000mg/L after being inoculated to a reactor. The sequencing batch reactor is a columnar reactor, the height-diameter ratio of the sequencing batch reactor is 4-20, the settling time of the reactor is controlled within 5 minutes, and the apparent ascending gas velocity is more than 1.2 cm/s. The influent water is gradually changed into inorganic high ammonia nitrogen wastewater from organic sewage in the process of acclimating aerobic ammonia oxidation granular sludge, the inorganic high ammonia nitrogen wastewater provides a carbon source by sodium bicarbonate, the influent ammonia nitrogen is 60-320 mg/L, and the pH is 6-9. The high-efficiency nitrobacteria concentrated solution is a nitrobacteria concentrated solution used for cleaning aquaria on the market, the contained bacteria are aerobic ammonia oxidizing bacteria, and the number of active nitrobacteria per milliliter of bacterial solution is more than three hundred million. The minimum adding amount of the high-efficiency nitrobacteria concentrated solution is 1 ml of bacteria solution/liter of reactor mixed solution, and the adding time is continued until the accumulation rate of the effluent nitrite nitrogen in the reactor exceeds 90%. The successful acclimation mark of the aerobic ammonia oxidation granular sludge is as follows: the SVI of the granular sludge is kept below 50mL/g, the granulation rate is kept above 90%, and the nitrite nitrogen accumulation rate and the removal rate of the influent ammonia nitrogen are both kept above 90%. The heterotrophic bacteria in the ammoxidation granular sludge are nitrosomonas.
The invention makes full use of the biological carrier function of the heterotrophic granular sludge and provides a large number of attachment points for the growth of autotrophic nitrifying bacteria. Meanwhile, the defect of slow proliferation rate of nitrobacteria is overcome by adding exogenous aerobic ammonia oxidizing bacteria, rapid enrichment of the aerobic ammonia oxidizing bacteria in the heterotrophic granular sludge is realized under a large selective pressure, the advantages of good settling property, high toxicity resistance and the like of AGS are fully exerted, and technical support is provided for harmless treatment of wastewater. Compared with other inoculated AGS domesticated autotrophic nitrification granular sludge, the method provided by the invention can effectively reduce the impact of the sharp change of the operation condition on the stability of the inoculated AGS, and realize the replacement of heterotrophic bacteria in granules by aerobic ammonia oxidizing bacteria in a short time.
Drawings
FIG. 1 is a diagram showing the effect of aerobic ammonia oxidation granular sludge on ammonia nitrogen removal in a small-scale SBR;
FIG. 2 is acclimatization and flora composition of aerobic ammonia oxidation granular sludge in a pilot SBR;
FIG. 3 is a diagram showing the effect of aerobic ammonia oxidation granular sludge on ammonia nitrogen removal in pilot SBR;
FIG. 4 shows acclimatization and bacterial flora composition of aerobic ammoxidation granular sludge in pilot SBR.
Detailed Description
A method for quickly culturing the aerobic granular ammonia oxidizing sludge includes inoculating the aerobic granular sludge containing heterotrophic bacteria to Sequencing Batch Reactor (SBR) as carrier, and starting SBR. The SBR is a columnar reactor, and the height-diameter ratio of the SBR is 4-20. The heterotrophic bacteria in the ammoxidation granular sludge are nitrosomonas. The dominant bacteria inoculated into the aerobic granular sludge in the embodiment belong to zoogloea. After the inoculation to the reactor, the initial reactor MLSS of the reactor is more than or equal to 3000mg/L, the apparent ascending gas velocity is more than or equal to 1.2cm/s, and the settling time is less than or equal to 5 minutes. In order to accelerate the formation of the autotrophic nitrification granular sludge, the aerobic ammonia oxidation granular sludge is domesticated by gradually reducing the COD value of the inlet water until the inlet water is completely replaced by the inorganic carbon source, the reduction range of the COD of the inlet water is determined by the granulation rate of the sludge, namely the COD of the inlet water is reduced by 100mg/L every day when the granulation rate is more than or equal to 80 percent, otherwise, the COD of the inlet water is kept unchanged, and the regulation and control are repeated until the COD of the inlet water is completely replaced by the inorganic carbon source, and the concentration of organic matters is zero. The influent water is gradually changed into inorganic high ammonia nitrogen wastewater from organic sewage in the process of acclimating aerobic ammonia oxidation granular sludge, the inorganic high ammonia nitrogen wastewater provides a carbon source by sodium bicarbonate, and the influent ammonia nitrogen is 60-320 mg/L. And in the acclimation process, the pH of inlet water is kept between 7 and 9, and TN (total nitrogen content) and TP (total phosphorus content) are unchanged. And (3) adding exogenous high-efficiency aerobic ammonia oxidizing bacteria concentrated solution (the number of active nitrifying bacteria per milliliter of bacterial solution is more than three hundred million) into the SBR while reducing the COD of the inlet water so as to promote the rapid enrichment of the aerobic ammonia oxidizing bacteria in the AGS and finally rapidly culture the aerobic ammonia oxidizing granular sludge capable of bearing high ammonia nitrogen load. The adding amount of the high-efficiency aerobic ammonia oxidizing bacteria concentrated solution is 1 ml of bacteria solution/liter of reactor mixed solution, the concentrated solution is injected into the reactor once along with inlet water in each period, and the adding time lasts until the accumulation rate of nitrite nitrogen in outlet water of the reactor exceeds 90%.
The successful culture mark of the aerobic ammonia oxidation granular sludge is as follows: the SVI of the granular sludge is kept below 50mL/g, and the granulation rate is kept above 90%; the accumulation rate of nitrite nitrogen and the removal rate of ammonia nitrogen in the inlet water are both kept above 90%. And then the ammonia nitrogen concentration or the nitrogen load of the inlet water can be changed according to the requirements to realize different treatment targets.
Case 1
And inoculating AGS to start the small-scale SBR (effective height of 180cm, inner diameter of 10.5cm, height-diameter ratio of 17 and effective volume of 15.58L), wherein the apparent ascending gas velocity is 1.2-1.5 cm/s, the water change rate is 50%, 6h is a cycle, and the settling time is 2 minutes. The inoculated sludge is AGS which is actually used for stably running the septic tank waste water, the color is bright yellow, the shape is mostly spherical or ellipsoidal, the initial MLSS is 4000mg/L, the MLVSS/MLSS is 0.56, the SVI is 40mg/L, and the EPS and the PN/PS are respectively 25.1mg/g MLSS and 0.22. The pH of the feed water was maintained at 7.The COD of the inlet water is reduced to zero from 600mg/L within 6 days between 5 and 8.7, the ammonia nitrogen of the inlet water is 140mg/L, and the nitrogen load is 0.28kg N/m3D. The nitrifying bacteria concentrated solution is purchased from a net, the main component is aerobic ammonia oxidizing bacteria, and the effective microorganism content is more than or equal to 10 x 109CFU/mL. After continuously adding the nitrobacteria concentrated solution for 1-20 days, measuring that NAR in the reactor is kept above 90%; after the reactor is operated for 31 days, the removal rate of ammonia nitrogen in the reactor is increased to 90 percent, as shown in figure 1, and the relative abundance of aerobic ammonia oxidizing bacteria reaches 27.87 percent, as shown in figure 2.
Case 2
The AGS is inoculated to start the pilot SBR (the effective height is 180cm, the inner diameter is 29.2cm, the height-diameter ratio is 6, the effective volume is 120.5L), the apparent gas velocity is 1.25cm/s, the water change rate is 60 percent, 6h is a period, and the settling time is 5 minutes. The inoculated sludge is AGS which is actually used for stably running the septic tank waste water, the color is bright yellow, the shape is mostly spherical or ellipsoidal, the initial MLSS is 4000mg/L, the MLVSS/MLSS is 0.56, the SVI is 40mg/L, and the EPS and the PN/PS are respectively 25.1mg/g MLSS and 0.22. The pH value of the inlet water is maintained between 6 and 9, the COD of the inlet water is reduced to zero from 600mg/L within 10 days, the ammonia nitrogen of the inlet water is 167mg/L, and the nitrogen load is 0.4kg N/m3D. The nitrifying bacteria concentrated solution is purchased from a net, the main component is aerobic ammonia oxidizing bacteria, and the effective microorganism content is more than or equal to 10 x 106CFU/mL. Continuously adding the nitrobacteria concentrated solution within 1-14 days, and measuring that the NAR in the reactor is increased to more than 90% after 14 days; after the operation for 37 days, the removal rate of ammonia nitrogen in the reactor is increased to 90 percent, as shown in figure 3, and the relative abundance of aerobic ammonia oxidizing bacteria reaches 17.27 percent, as shown in figure 4.
The method can effectively reduce the impact of the sharp change of the operation condition on the stability of the inoculated heterotrophic granular sludge, can realize the replacement of heterotrophic bacteria in the granules by aerobic ammonia oxidizing bacteria in about one month, and greatly shortens the time required by the culture of the autotrophic nitrification granular sludge. The cultured aerobic ammonia oxidation granular sludge can bear high ammonia nitrogen load, realizes stable shortcut nitrification, and provides a new idea for removing ammonia nitrogen in mine wastewater in China.
Claims (6)
1. A fast culture method of aerobic ammonia oxidation granular sludge is characterized in that aerobic granular sludge mainly containing heterotrophic bacteria is inoculated into a sequencing batch reactor to be used as a carrier, then the aerobic ammonia oxidation granular sludge is acclimated by gradually reducing the COD value of inlet water to zero concentration of organic matters, and a high-efficiency nitrobacteria concentrated solution is added in the acclimation process to promote the fast enrichment of autotrophic bacteria in the granular sludge, so that the aerobic ammonia oxidation granular sludge is cultured fast; the influent water is gradually changed into inorganic high ammonia nitrogen wastewater from organic sewage in the process of acclimating aerobic ammonia oxidation granular sludge, the inorganic high ammonia nitrogen wastewater provides a carbon source by sodium bicarbonate, the influent ammonia nitrogen is 60-320 mg/L, and the pH is 6-9; the high-efficiency nitrobacteria concentrated solution is a nitrobacteria concentrated solution used for cleaning aquaria on the market, the contained bacteria are aerobic ammonia oxidizing bacteria, and the number of active nitrobacteria per milliliter of bacterial solution is more than three hundred million.
2. The method for rapidly culturing the aerobic ammonia oxidation granular sludge as claimed in claim 1, wherein the aerobic granular sludge mainly containing heterotrophic bacteria is inoculated, the autotrophic bacteria ratio is less than or equal to 10%, the sedimentation speed is greater than or equal to 15m/h, and the MLSS is greater than or equal to 3000mg/L after the aerobic granular sludge is inoculated to the reactor.
3. The method of claim 1, wherein the sequencing batch reactor is a cylindrical reactor, the aspect ratio of the reactor is 4 to 20, the settling time of the reactor is controlled within 5 minutes, and the superficial aeration rate is 1.2cm/s or more.
4. The method for rapidly culturing the aerobic ammonia oxidation granular sludge according to claim 1, wherein the minimum adding amount of the high-efficiency nitrifying bacteria concentrated solution is 1 ml of bacteria solution per liter of the mixed solution of the reactor, and the adding time lasts until the accumulation rate of nitrite nitrogen in effluent of the reactor exceeds 90%.
5. The method for rapidly cultivating the aerobic ammonia oxidation granular sludge according to claim 1, wherein the successful acclimation mark of the aerobic ammonia oxidation granular sludge is as follows: the SVI of the granular sludge is kept below 50mL/g, the granulation rate is kept above 90%, and the nitrite nitrogen accumulation rate and the removal rate of the influent ammonia nitrogen are both kept above 90%.
6. The method for rapidly culturing aerobic ammonia oxidation granular sludge according to claim 1, wherein the dominant bacteria of the autotrophic bacteria in the aerobic ammonia oxidation granular sludge is nitrosomonas.
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| CN102259976B (en) * | 2011-05-23 | 2013-01-23 | 杭州师范大学 | Method for quickly starting anaerobic ammonia oxidation reactor |
| KR101336988B1 (en) * | 2011-09-30 | 2013-12-05 | 주식회사 블루뱅크 | Wastewater Treatment Apparatus Using Granule Sludge and Method for Treating Wastewater Using the Same |
| CN103043788B (en) * | 2013-01-04 | 2013-12-11 | 哈尔滨工业大学 | Cultivation method of anammox granular sludge |
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| CN107892385B (en) * | 2017-11-22 | 2020-10-16 | 沈阳建筑大学 | Reinforced culture method for partial nitrosation granular sludge at low temperature |
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