CN110252786A - Application of the earthworm in degradation DEHP - Google Patents
Application of the earthworm in degradation DEHP Download PDFInfo
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- CN110252786A CN110252786A CN201910419748.8A CN201910419748A CN110252786A CN 110252786 A CN110252786 A CN 110252786A CN 201910419748 A CN201910419748 A CN 201910419748A CN 110252786 A CN110252786 A CN 110252786A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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Abstract
The present invention provides a kind of application of earthworm in degradation DEHP.Experiment discovery can effectively be degraded the DEHP in soil, especially joint pseudomonad using earthwormPseudomonas Sp.9-8 is 3. and earthworm excrement uses simultaneously, has higher degradation efficiency, provides effective scheme to improve soil.
Description
Technical field
The present invention relates to a kind of application of earthworm in degradation DEHP.
Background technique
Soil is the material base of the sustainable development of socio-economy, relationship body health of people, the beautiful China of relationship
Construction, protecting soil environment is the important content for promoting Ecological Civilization Construction and safeguarding National Ecological Security.In recent years, with
The fast development of industrialization and urbanization, China's soil pollution are on the rise.What Chinese Ministry of Environmental Protection in 2014 and Ministry of Land and Resources issued
" national Soil Pollution Investigation bulletin " shows that arable soil environmental quality in China's causes anxiety, and 19.4% arable land point is exceeded,
Pollution cultivated area reaches 2.3 × 107hm2.Soil pollution has seriously endangered China's agricultural land soil environmental quality and agricultural product peace
Entirely.
Plasticiser is the high molecular material auxiliary agent being widely used in industrial production, also known as plasticizer.Phthalic acid
Esters (PAEs) are the main bodys of plasticiser, and yield accounts for about the 80% of plasticiser total output, the residual quantity in surrounding medium
Constantly accumulation, it has also become global typical contamination object.And DEHP (phthalic acid -2 (ethylhexyl) ester) usage amount is more than
The ninety percent of PAEs.DEHP with plastic matrix is intermolecular is only connected with hydrogen bond or Van der Waals force, easily revealed from plastic products
Out, it constantly releases into atmosphere, soil and waters, causes the harm to environment, to cause potential prestige to human health
The side of body.Currently, plasticiser has become most commonly used environmental contaminants on the earth, atmosphere, lake, river and the soil of many countries
In detected the plasticiser of various concentration, it has also become the organic pollutant being most often detected in soil.The toxicity master of plasticiser
It is embodied in genetoxic, embryonic development toxicity, genotoxicity, neurotoxicity and immunotoxicity etc..
In view of DEHP to the seriousness and harmfulness of soil pollution, contaminated soil remediation technology causes the whole world and generally closes
Note.Currently, microorganism remediation technology is a kind of generally acknowledged effective, safe, cheap and without secondary pollution method.But DEHP
The microorganism remediation technology of contaminated soil faces many restrictions factor in an implementation: first is that inoculating microbes microorganism is in soil environment
Middle bad adaptability, activity is low, and migration is poor: second is that in the complex system of soil multiphase multiple solutions, the chlorine with hydrophobic lipophilic
It is easily adsorbed and is fixed by organic aggregate etc. for aromatic hydrocarbons and zymoprotein, reduce solubility, biological effectiveness and reaction contact.But
For the other methods such as physics, chemistry, a kind of inexpensive, environmental-friendly biological actuation techniques raising microorganism is studied
Degradation efficiency become contaminated soil in-situ immobilization important content.
Typical Representative of the earthworm as soil animal has " raw in occupation of the major part of terrestrial ecosystems biomass
The good reputation of state system engineer ".Earthworm is widely distributed in all over the world, has extremely strong adaptive capacity to environment and fertility,
There are higher patience, bioaccumulation and resistance to pollution stress.Existing research shows that influence of the earthworm movement to soil helps
It is passivated in soil pollutant such as heavy metal, the removal of pesticide, polycyclic aromatic hydrocarbon, Polychlorinated biphenyls.But its degradation to organic pollutant
The influence of conversion is also related with soil types, pollutant kind and earthworm type.
Microorganism plays very important effect in degradable organic pollutant, so earthworm is added into contaminated soil to be had
Help the degradation of organic pollutant.The efficient degrading bacteria that usual laboratory screening comes out, migration and diffusivity are poor, colonize energy
Power is limited.But effect report of the earthworm in soil plasticiser, especially phthalate pollution amelioration is very limited,
It needs further to study.
Summary of the invention
In view of the foregoing deficiencies of prior art, the purpose of the present invention is to provide it is a kind of for earthworm degradation DEHP in
Application, for solving the problems, such as that DEHP degradation is difficult in the prior art.
In order to achieve the above objects and other related objects, the present invention provides application of the earthworm in degradation DEHP.
Earthworm, Pheretima, Oligochaeta Oligochaeta, unidirectional earthworm suborder.
Further, the earthworm is power simulation, Pheretima guillelmi (Michaelsen, 1895).
Another aspect of the present invention provides application of the earthworm in the composition that preparation has degradation DEHP function.
Another aspect of the present invention, which provides earthworm, to be had preparing the application in agricultural formulations, the agricultural formulations
The effect of degradation DEHP.
Further, the agricultural formulations can be dedicated for the preparation for the DEHP that degrades, or also have other simultaneously
The preparation of function.
3. another aspect of the present invention provides a kind of pseudomonad Pseudomonas sp.9-8, the vacation unit cell
The deposit number of bacterium Pseudomonas sp.9-8 3. is CCTCC NO:M2019158.
This bacterial strain is screened in Shanghai farmland soil for inventor and is obtained.The bacterial strain is on March 15th, 2019 in China
Type Tissue Collection (abbreviation CCTCC) registers preservation, and deposit number is CCTCC NO:M2019158.
The bacterial strain delivers Zhejiang University's extremophile microorganism laboratory in December, 2018 and carries out identification by morphological characters, identification knot
Fruit are as follows:
Morphological feature: 30 DEG C incubator culture 2-3 days, colony size is small, rounded protrusion, regular edges, translucent Huang
Color, surface are smooth；Gram-negative；Thallus is elongated rod shape, and size is (0.86~3.95) μ m (0.25~0.61) μm.
Physiological property: sugar fermentation and catalase test are positive；Esterase (C4), esterase lipase (C8), leucine arylamine enzyme,
Naphthol-AS-BI-phosphohydrolase is positive；Nitrate, arginine dihydrolase, urase, β-Portugal can be restored
Polyglycoside hydrolase, proteolytic enzyme, beta galactosidase are the positive.
Through Morphological Identification and the development tree analysis of 16srRNA extension increasing sequence, determine that the bacterial strain is pseudomonad, according to the world
Naming rule: generic name+kind of name+strain name is named the bacterial strain, is named as Pseudomonas sp.9-8 3..
Further, the nucleotide sequence of the pseudomonad Pseudomonas sp.9-8 3. such as SEQ ID NO.1 institute
Show.
Further, 3. the pseudomonad Pseudomonas sp.9-8 has the function of the DEHP that degrades.
3. another aspect of the present invention provides above-mentioned pseudomonad Pseudomonas sp.9-8 to be had in preparation
Application in the composition for DEHP function of degrading.
Another aspect of the present invention provides pseudomonad Pseudomonas sp.9-8 and is 3. preparing agricultural formulations
In application, the agricultural formulations have the function of degrade DEHP.
Further, the agricultural formulations can be dedicated for the preparation for the DEHP that degrades, or also have other simultaneously
The preparation of function.
Another aspect of the present invention provides application of the earthworm excrement in degradation DEHP.
Another aspect of the present invention provides application of the earthworm excrement in the composition that preparation has degradation DEHP function.
Another aspect of the present invention, which provides earthworm excrement, to be had preparing the application in agricultural formulations, the agricultural formulations
The effect of degradation DEHP.
Further, the agricultural formulations can be dedicated for the preparation for the DEHP that degrades, or also have other simultaneously
The preparation of function.
Further, the earthworm excrement is to originate from power simulation.
Another aspect of the present invention provides a kind of composition with degradation DEHP function, contains in the composition
There is above-mentioned earthworm.
Another aspect of the present invention provides a kind of agricultural formulations with degradation DEHP function, the agricultural formulations
In contain earthworm and carrier.
Further, the carrier is soil.
Further, also contain pseudomonad Pseudomonas sp.9-8 3. in the agricultural formulations.
Further, earthworm excrement is also contained in the agricultural formulations.
Another aspect of the present invention provides the preparation method of above-mentioned agricultural formulations or composition, the method includes
Each component is mixed.
Another aspect of the present invention provides the use of above-mentioned composition or agricultural formulations for DEHP in soil of degrading
On the way.
Another aspect of the present invention provide it is a kind of degradation soil in DEHP method, the method includes to soil
It is middle to apply above-mentioned earthworm, composition and/or agricultural formulations.
Further, which comprises while applying pseudomonad Pseudomonas sp.9-8 3. into soil.
Further, which comprises while applying earthworm excrement into soil.
As described above, the application of earthworm of the invention in degradation DEHP, has the advantages that
Experiment discovery can be degraded the DEHP in soil using earthworm, for improving soil efficiency with higher, and not
Secondary pollution can be brought to environment.Experiment further proves, by adding earthworm, Pseudomonas into soil simultaneously
Sp.9-8 is 3. and earthworm excrement has higher degradation efficiency, achievees the effect that synergistic effect.
Bacterial strain preservation information of the present invention is as follows:
Strain name: pseudomonad Pseudomonas sp.9-8 is 3.；
Deposit number are as follows: CCTCC NO:M2019158；
Preservation date: on March 15th, 2019；
Depositary institution's title: China typical culture collection center；
Depositary institution's abbreviation: CCTCC；
Depositary institution address: wuchang, wuhan Luo Jia Shan street Wuhan University Life Science College.
Detailed description of the invention
Fig. 1 is shown as 1 result schematic diagram of the embodiment of the present invention.
Fig. 2 is shown as 2 result schematic diagram of the embodiment of the present invention.
Fig. 3 is shown as 3 result schematic diagram of the embodiment of the present invention.
Fig. 4 is shown as 4 result schematic diagram of the embodiment of the present invention.
Fig. 5 is shown as 5 result schematic diagram of the embodiment of the present invention.
Fig. 6 is shown as 6 result schematic diagram of the embodiment of the present invention.
Fig. 7 is shown as in the present invention Pseudomonas sp.9-8 3. first scanning electron microscope (SEM) photograph.
Fig. 8 is shown as in the present invention Pseudomonas sp.9-8 3. second scanning electron microscope (SEM) photograph.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.
Material and source:
Earthworm (power simulation): Shanghai Chongming farmland.
Pseudomonas sp.9-8 is 3.: screening obtains in Shanghai farmland soil.Shanghai farmland contaminated soil 10g is taken to be put into
To 200ml using DEHP as in the enriched liquid culture medium of sole carbon source, at 30 DEG C, 200rpm shaken cultivation 5d.Take above-mentioned culture
Liquid 10ml is transferred in fresh enriched liquid culture medium, is transferred 3-5 times.Take enrichment culture liquid 1ml that 10-1~10- is successively made
The dilution of 10 dilutions is coated on using DEHP as on the solid separation culture medium plate of sole carbon source, inversion is put in 30 DEG C
In constant incubator.Longer positive bacterium colony is transferred on solid separation culture medium plate with plate streak, is inverted training
It supports.Repeat this operation 2-3 times.
The bacterial strain registers preservation at China typical culture collection center (abbreviation CCTCC) on March 15th, 2019, protects
Hiding number is CCTCC NO:M2019158.
The bacterial strain delivers Zhejiang University's extremophile microorganism laboratory in December, 2018 and carries out identification by morphological characters, identification knot
Fruit are as follows:
Morphological feature: 30 DEG C incubator culture 2-3 days, colony size is small, rounded protrusion, regular edges, translucent Huang
Color, surface are smooth；Gram-negative；Thallus is elongated rod shape, and size is (0.86~3.95) μ m (0.25~0.61) μm.
Physiological property: sugar fermentation and catalase test are positive；Esterase (C4), esterase lipase (C8), leucine arylamine enzyme,
Naphthol-AS-BI-phosphohydrolase is positive；Nitrate, arginine dihydrolase, urase, β-Portugal can be restored
Polyglycoside hydrolase, proteolytic enzyme, beta galactosidase are the positive.
16S rDNA sequence: SEQ ID NO.1
tggggggcggcagcctacacatgcaagtcgagcggtagagagaagcttgcttctcttgagagcggcgg
acgggtgagtaatgcctaggaatctgcctggtagtgggggataacgttcggaaacggacgctaataccgcatacgt
cctacgggagaaagcaggggaccttcgggccttgcgctatcagatgagcctaggtcggattagctagttggtgagg
taatggctcaccaaggcgacgatccgtaactggtctgagaggatgatcagtcacactggaactgagacacggtcca
gactcctacgggaggcagcagtggggaatattggacaatgggcgaaagcctgatccagccatgccgcgtgtgtgaa
gaaggtcttcggattgtaaagcactttaagttgggaggaagggttgtagattaatactctgcaattttgacgttac
cgacagaataagcaccggctaactctgtgccagcagccgcggtaatacagagggtgcaagcgttaatcggaattac
tgggcgtaaagcgcgcgtaggtggtttgttaagttggatgtgaaatccccgggctcaacctgggaactgcattcaa
aactgactgactagagtatggtagagggtggtggaatttcctgtgtagcggtgaaatgcgtagatataggaaggaa
caccagtggcgaaggcgaccacctggactaatactgacactgaggtgcgaaagcgtggggagcaaacaggattaga
taccctggtagtccacgccgtaaacgatgtcaactagccgttggaagccttgagcttttagtggcgcagctaacgc
attaagttgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtg
gagcatgtggtttaattcgaagcaacgcgaagaaccttaccaggccttgacatccaatgaactttctagagataga
ttggtgccttcgggaacattgagacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtc
ccgtaacgagcgcaacccttgtccttagttaccagcacgtaatggtgggcactctaaggagactgccggtgacaaa
ccggaggaaggtggggatgacgtcaagtcatcatggcccttacggcctgggctacacacgtgctacaatggtcggt
acagagggttgccaagccgcgaggtggagctaatcccataaaaccgatcgtagtccggatcgcagtctgcaactcg
actgcgtgaagtcggaatcgctagtaatcgcgaatcagaatgtcgcggtgaatacgttcccgggccttgtacacac
cgcccgtcacaccatgggagtgggttgcaccagaagtagctagtctaaccttcgggaggacggtaccacggtgatc
gcgg
Scanning electron microscope such as Fig. 7 and Fig. 8.
The Pseudomonas is in Proteobacteria Proteobacteria, γ-deformation Gammaproteobacteria Gammaproteobacteria, false unit cell
Zoopagales Pseudomonadales, pseudomonadaceae Pseudomonadaceae, pseudomonas Pseudomonas.
1 earthworm of embodiment degrades to DEHP
Contamination process: accurately weighing 0.12g DEHP and be dissolved in ethanol in proper amount, is uniformly sprayed in soil and stirs
Even, avoid light place for 24 hours, makes ethyl alcohol volatilize completely.The 40%-60% for adjusting soil moisture to maximum water-holding capacity, and if being sub-packed in
In dry experiment basin, 4 groups are formed as process object, every group of soil sample 1kg, DEHP concentration is 10mg/kg.4 groups of process objects:
CK: sterile soil,
Q-4:3-4 item/kg soil,
Q-6:5-6 item/kg soil,
Q-2:1-2 item/kg soil.
Each processing is in triplicate.
DEHP residual quantity in results of regular determination soil.It is put into the constant temperature biochemical cultivation case that temperature is 18-25 DEG C and cultivates, cultivate
Time 35 days.
Effect is shown in Fig. 1: adding DEHP degradation rate in the processing of earthworm and is significantly higher than control (p < 0.05).DEHP in CK processing
Concentration variation is negligible.When 15d, DEHP degradation rate is respectively 0.00%, 48.66% and in Q-2, Q-4 and Q-6 processing
57.82%.The DEHP of possible initial concentration 10mg/kg still has certain toxic action to earthworm, adds the place of 2 earthworms
DEHP concentration is almost unchanged in reason group.When 35d, Q-2, Q-4 and Q-6 processing in DEHP concentration be respectively 0.80mg/kg,
0.57mg/kg and 0.1mg/kg, degradation rate are respectively 87.36%, 91.00% and 98.42%；Illustrate that earthworm has gradually adapted to ring
Border variation, and the DEHP in environment is eliminated quickly.As it can be seen that handling DEHP efficiency also phase with the increase of earthworm density
It should increase, 5-6 item/kg soil treatment group effect is best.
3. 2 Pseudomonas sp.9-8 of embodiment degrades to DEHP
Contamination process: accurately weighing 0.042g DEHP and be dissolved in ethanol in proper amount, is uniformly sprayed in soil and stirs
Even, avoid light place for 24 hours, makes ethyl alcohol volatilize completely.The 40%-60% for adjusting soil moisture to maximum water-holding capacity, and if being sub-packed in
In dry experiment basin, 2 groups of soil are formed as process object, every group of soil sample 100g, DEHP concentration is 70mg/kg.2 groups of experiment process
Object:
CK: sterile soil.
9-8 is 3.: 5mL microbial bacterial agent (about 1012cfu/ml)。
Each processing is in triplicate.
DEHP residual quantity in results of regular determination soil.It is put into the constant temperature biochemical cultivation case that temperature is 18-25 DEG C and cultivates, cultivate
Time 35 days.
Effect is shown in Fig. 2: pollutant in soil degradation it is not only related with the degradation capability of microorganism, also with the suction of pollutant
Attached, parsing, Transport And Transformation, available state etc. are related.Microbial bacterial agent processing in DEHP degradation rate be apparently higher than control group (p <
0.05).DEHP concentration variation in CK processing is negligible.After the shorter retardation laundering period, 3. 9-8 starts degradation soil
DEHP in earth.When 7d, 3. 9-8 is 37.40% to the degradation rate of DEHP.When 30d, DEHP is remained in microbial bacterial agent processing group
Amount is 13.01mg/kg, degradation rate 76.46%.As it can be seen that microbial bacterial agent has preferably the removal of DEHP in soil
Effect.
The degradation of 3 earthworm excrement DEHP of embodiment
Contamination process such as embodiment 1.
It is respectively 0g, 25g, 50g, 75g, 100g for examination earthworm excrement additional amount, is repeated 3 times, totally 15 processing.Each processing soil
Earth gross weight is 100g, and insufficient person is supplied using pure pedotheque, and DEHP concentration is 70mg/kg.Earthworm excrement and pedotheque is abundant
It mixes, water content is maintained at 50-60%.Measure the variation of DEHP residual quantity in soil.
Effect is shown in Fig. 3.Earthworm fecal energy is effectively degraded DEHP in soil.When 10d, 0g, 25g, 50g, 75g, the processing of 100g earthworm excrement
It is 4.80%, 30.38%, 43.65%, 55.01%, 62.32% to DEHP degradation rate；And when earthworm excrement additive amount be 50g, 75g,
When 100g, in 20d, the degradation rate of 30d respectively greater than 78% and 84% is significant with 0g and 25g processing difference.It can be seen that
With the increase of earthworm excrement amount, the degradation rate of DEHP is also with increase in soil, but difference is smaller, and reason may is that DEHP belongs to
Hydrophobic compound, bioavailability may belong to limiting factor at this moment.25g earthworm excrement processing group is in 20d and 30d to DEHP
Degradation rate be respectively 52.04% and 74.21%；And the fluctuation of 0g earthworm excrement processing group DEHP may be by experimental error and DEHP
It is inevitable volatilization caused by.Comprehensively considering output ratio both can guarantee organic contamination when the addition of earthworm excrement is than being 1:1 (50g)
The degradation efficiency of object, and can investment reduction cost to the full extent.
3. 4 earthworm of embodiment joint microbial bacterial agent Pseudomonas sp.9-8 degrades DEHP
Contamination process: each experimental group and the soil treatment mode of control are same as Example 1.Finally 3 groups of tests of setting
And 1 group of control:
CK: sterile soil,
Q-4:3-4 item/kg soil,
9-8 is 3.: addition microbial bacterial agent,
Q-4+9-8 is 3.: earthworm+microbial bacterial agent.
Each processing is in triplicate.
It is put into the constant temperature biochemical cultivation case that temperature is 18-25 DEG C and cultivates 35 days.DEHP residual quantity in results of regular determination soil.
Effect is shown in Fig. 4: addition earthworm or (and) in the processing of microbial bacterial agent DEHP degradation rate be significantly higher than control (p <
0.05).The variation of DEHP concentration is negligible in CK processing.When 35d, 9-8 3., Q-4 and Q-4+9-8 3. handle in DEHP concentration
Respectively 0.87mg/kg, 0.57mg/kg and 0.25mg/kg, degradation rate are respectively 86.26%, 91.00% and 96.05%.It is micro-
Bacteria agent and earthworm have a more significant degradation effect to DEHP, and sequence be Q-4+9-8 3. > Q-4 > 9-8 3. > CK.And
By experimental data it can be seen that earthworm joint microorganism produces synergistic effect.
3. 5 earthworm excrement of embodiment joint microbial bacterial agent Pseudomonas sp.9-8 degrades DEHP
Contamination process such as embodiment 1.Each processing soil sample 0.5kg, DEHP concentration is 70mg/kg, earthworm excrement or microbial bacteria
To guarantee to stir evenly in agent adding procedure.
Experimental design totally four processing:
CK: sterile soil,
YF: the processing of earthworm excrement,
CM: only adding microbial bacterial agent,
YC: earthworm excrement+microbial bacterial agent.
Each processing is in triplicate.
As a result as shown in figure 5, addition earthworm excrement and microbial bacterial agent processing in DEHP degradation rate be significantly higher than control (p <
0.05).The variation of DEHP concentration is negligible in CK processing.When 30d, DEHP concentration is respectively in CM, YF and YC processing
13.01mg/kg, 5.76mg/kg and 1.03mg/kg, degradation rate are respectively 76.46%, 89.58% and 97.65%.Thus may be used
See, the processing of earthworm excrement will be handled slightly better than microbial bacterial agent, and the treatment effect of earthworm excrement joint microbial bacterial agent is best.
6 earthworm excrement of embodiment combine microbial bacterial agent Pseudomonas sp.9-8 3. and earthworm degrade DEHP
Contamination process such as embodiment 1.Each processing soil sample 1kg, DEHP concentration is 10mg/kg, earthworm excrement or microbial bacterial agent
To guarantee to stir evenly in adding procedure.
Experimental design totally 4 processing:
CK: sterile soil
9-8 is 3.: only plus microbial bacterial agent
YF: earthworm excrement processing
MIX: earthworm excrement+microbial bacterial agent+earthworm
Each processing is in triplicate.
Processing result is as shown in fig. 6, add DEHP in earthworm excrement/microbial bacterial agent/earthworm excrement+microbial bacterial agent+dealing with earthworm
Degradation rate is significantly higher than control (p < 0.05).DEHP concentration variation in CK processing is negligible.It is micro- from the point of view of degradation initial stage
The degradation effect of bacteria agent processing group however as YF and MIX treatment effect is obvious, reason may is that single bacterium is not so good as mixed bacterium effect
Fruit is good, and the movement migration effect of bacterium is poor.As incubation time extends, different disposal group can be removed preferably in soil
DEHP.When 30d, 3., DEHP concentration is respectively 1.01mg/kg, 0.68mg/kg, 0.01mg/kg to 9-8 in YF and MIX processing, drop
Solution rate is respectively 85.31%, 90.11%, 99.85%.It can be seen that earthworm excrement+microbial bacterial agent+dealing with earthworm better than earthworm excrement+
Microbial bacterial agent processing, and earthworm excrement+microbial bacterial agent treatment effect is better than microbial bacterial agent processing.
Above embodiment is can not to be interpreted as in order to illustrate embodiment disclosed by the invention to limit of the invention
System.In addition, in various modifications and invention listed herein method, composition variation, do not departing from the scope of the present invention
Be obvious for those skilled in the art under the premise of spirit.Although having combined of the invention a variety of specific
Preferred embodiment has carried out specific description to the present invention, it is to be understood that, the present invention should not be limited only to these specific embodiments.
In fact, various obviously modify as described above for those skilled in the art to obtain invention all should include
Within the scope of the invention.
Sequence table
<110>Academy of Agricultural Sciences, Shanghai City
<120>application of the earthworm in degradation DEHP
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1440
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
tggggggcgg cagcctacac atgcaagtcg agcggtagag agaagcttgc ttctcttgag 60
agcggcggac gggtgagtaa tgcctaggaa tctgcctggt agtgggggat aacgttcgga 120
aacggacgct aataccgcat acgtcctacg ggagaaagca ggggaccttc gggccttgcg 180
ctatcagatg agcctaggtc ggattagcta gttggtgagg taatggctca ccaaggcgac 240
gatccgtaac tggtctgaga ggatgatcag tcacactgga actgagacac ggtccagact 300
cctacgggag gcagcagtgg ggaatattgg acaatgggcg aaagcctgat ccagccatgc 360
cgcgtgtgtg aagaaggtct tcggattgta aagcacttta agttgggagg aagggttgta 420
gattaatact ctgcaatttt gacgttaccg acagaataag caccggctaa ctctgtgcca 480
gcagccgcgg taatacagag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcgc 540
gtaggtggtt tgttaagttg gatgtgaaat ccccgggctc aacctgggaa ctgcattcaa 600
aactgactga ctagagtatg gtagagggtg gtggaatttc ctgtgtagcg gtgaaatgcg 660
tagatatagg aaggaacacc agtggcgaag gcgaccacct ggactaatac tgacactgag 720
gtgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat 780
gtcaactagc cgttggaagc cttgagcttt tagtggcgca gctaacgcat taagttgacc 840
gcctggggag tacggccgca aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc 900
ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggcc ttgacatcca 960
atgaactttc tagagataga ttggtgcctt cgggaacatt gagacaggtg ctgcatggct 1020
gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgt aacgagcgca acccttgtcc 1080
ttagttacca gcacgtaatg gtgggcactc taaggagact gccggtgaca aaccggagga 1140
aggtggggat gacgtcaagt catcatggcc cttacggcct gggctacaca cgtgctacaa 1200
tggtcggtac agagggttgc caagccgcga ggtggagcta atcccataaa accgatcgta 1260
gtccggatcg cagtctgcaa ctcgactgcg tgaagtcgga atcgctagta atcgcgaatc 1320
agaatgtcgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag 1380
tgggttgcac cagaagtagc tagtctaacc ttcgggagga cggtaccacg gtgatcgcgg 1440
Claims (9)
1. application of the earthworm in degradation DEHP.
2. application according to claim 1, it is characterised in that: the earthworm is power simulation.
3. application of the earthworm in the agricultural composition that preparation has degradation DEHP function.
4. earthworm is preparing the application in agricultural formulations, the agricultural formulations have the function of the DEHP that degrades.
5. a kind of method of DEHP in degradation soil, the method includes applying earthworm into soil, the agricultural containing earthworm is used
Composition and/or agricultural formulations.
6. method as claimed in claim 5, which is characterized in that the described method includes: applying pseudomonad into soil simultaneously
Pseudomonas sp.9-8 3., the deposit number of the pseudomonad Pseudomonas sp.9-8 3. are as follows: CCTCC NO:
M2019158。
7. according to the method described in claim 6, it is characterized in that, the core of the pseudomonad Pseudomonas sp.9-8 3.
Nucleotide sequence such as SEQ ID NO.1.
8. such as method described in claim 5 or 6, which is characterized in that the described method includes: applying earthworm excrement into soil simultaneously.
9. according to the method described in claim 8, it is characterized in that, the earthworm excrement originates from power simulation.
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