CN110248063A - A kind of colored coherent imaging device and method for no lens microscopic system - Google Patents
A kind of colored coherent imaging device and method for no lens microscopic system Download PDFInfo
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Abstract
The invention discloses a kind of colored coherent imaging devices and method for no lens microscopic system, belong to biomedical micro-imaging field, and described device includes: lighting unit, optic fiber coupling unit, filter unit and detection imaging unit；Lighting unit, for exporting red, green, blue three-color light source, and each light source is narrow-band light source；Optic fiber coupling unit, for the red, green, blue three-color light source to be coupled into same optical fiber；Filter unit carries out filter in spatial domain and frequency domain filtering for the complex light to the optical fiber output；Detection imaging unit for demodulating to three-color light source, and carries out image high-resolution reduction.Three color narrow-band light source of red, green, blue is coupled in same optical fiber by imaging device of the invention forms complex light, and eliminates color cross-talk by linear operation in demodulating process, can realize colour imaging on the basis of no mirror high-resolution single frames is imaged.
Description
Technical field
The invention belongs to biomedical micro-imaging field, more particularly, to a kind of for no lens microscopic system
Colored coherent imaging device and method.
Background technique
In biomedical detection process immediately, it is often necessary to analyze the morphology letter of the tiny samplers such as cell, microorganism
Fluoroscopic image information after breath and the biochemical reactions such as nucleic acid, antigen-antibody, and the acquisition of these information usually require to use it is aobvious
Micro- imaging device.Traditional optical microscopy is mainly made of light source, optical lens, photodetector three parts.Optical lens
Main function is that sample is carried out to optical amplifier and is focused it on photodetector to be imaged.But optical lens usually requires
It is used cooperatively with components such as optical tubes, aperture and focusing systems to obtain clearly image, considerably increases microscopical
Volume and complexity become microscope and are used for the big resistance that instant detection field must overcome.
Compared to conventional microscope, no lens microscope is easy with its big visual field and application as novel microscopic system
Characteristic favored in field of biomedicine.No lens imaging technology is by sample and charge coupled cell CCD or complementary gold
Belong to the photodetectors such as conductor oxidate CMOS chip to be in close contact, is not necessarily to optical element, the skill that directly sample is imaged
Art.Presently the most mainstream is relevant without lens micro imaging system without mirror system, carries out holographic photograph by using coherent source
It is bright, high stability can be brought for its micro-imaging, the depth of field expands to several millimeters from some tens of pm, and can be additional
The phase information of object is extracted, therefore, it has become main research directions.
However, relevant at present, without lens microscopic system, there is also certain disadvantages, must be used due to relevant without mirror system
Narrowband coherent source realizes holographic imaging, and the light source of illumination must be stringent monochromatic (within bandwidth ± 10nm), so at present
The relevant ability for not having colour imaging without lens microscopic system, but in many fields, in biomedical relevant application, coloured silk
Chromatic graph picture provides the additional information and contrast of sample, is therefore preferred research object.
In general, need to propose a kind of on the basis of the imaging of no mirror high-resolution single frames, the colored multichannel of realization at present
The device of imaging.
Summary of the invention
In view of the drawbacks of the prior art, the purpose of the present invention is to provide a kind of colored phases for no lens microscopic system
Dry imaging device and method, it is intended to solve it is relevant at present without lens microscopic system can only forming monochrome image, can not achieve colour imaging
The problem of.
To achieve the above object, one aspect of the present invention provides a kind of colored coherent imaging for no lens microscopic system
Device, comprising: lighting unit, optic fiber coupling unit, filter unit and detection imaging unit；
The lighting unit, for exporting three color narrow band light of red, green, blue；
The optic fiber coupling unit, for the three color narrowband of red, green, blue to be optically coupled into same optical fiber, output is multiple
Light combination；
The filter unit after carrying out filter in spatial domain and frequency domain filtering for the complex light to the optical fiber output, shines
It penetrates on sample；
The detection imaging unit, the initial pictures generated after irradiation for receiving the sample, and to described first
Beginning image carries out the reduction of demodulation high-resolution.
Further, the three color narrowband optical linewidth of red, green, blue is in 18nm between 35nm.
Further, the optic fiber coupling unit, including luminous source optical fiber coupling subelement and optical fiber combining coupling subelement；
The luminous source optical fiber couples subelement, for three color narrow band light of red, green, blue to be coupled into three optical fiber respectively；
The optical fiber combining coupling subelement, for three color narrow band lights in three optical fiber to be coupled into same optical fiber.
Further, the luminous source optical fiber coupling subelement is graded index rod-shaped lens；The optical fiber combining coupling
Subelement is the fiber coupler of 3x1.
Further, the filter unit carries out filter in spatial domain using the aperture of 50-100um magnitude, small using bandwidth
Carry out frequency domain filtering in or equal to the optical filter of ± 10nm, the aperture and optical filter are close to, and with the imaging detection list
The distance of member is between 3-5cm.
Further, during the imaging detection unit demodulates initial pictures, use following matrix to disappear
Except color cross-talk；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RbluePoint
Not Biao Shi RGB light practical two-dimensional intensity vector.
Further, the detection imaging unit is Foveon X3 sensor, and its pixel dimension is less than or equal to
2.2um。
Another aspect of the present invention provides a kind of colored coherent imaging method for no lens microscopic system, comprising:
(1) three color narrow-band light source of red, green, blue is coupled into same optical fiber, exports complex light；
(2) it is radiated on sample after the complex light of output being carried out filter in spatial domain and frequency domain filtering, obtains initial pictures；
(3) initial pictures are demodulated, obtains three width holograms；
(4) reduction of image high-resolution is carried out to three obtained width holograms, obtains color hologram.
Further, the three color narrowband optical linewidth of red, green, blue is in 18nm between 35nm.
Further, during demodulating to initial pictures, use following matrix to eliminate color cross-talk；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RbluePoint
Not Biao Shi RGB light practical two-dimensional intensity vector.
Contemplated above technical scheme through the invention can obtain following compared with prior art
The utility model has the advantages that
(1) three color narrow-band light source of red, green, blue is coupled in same optical fiber by the present invention forms complex light, and is demodulating
Color cross-talk is eliminated by linear operation in the process, can realize colour imaging on the basis of no mirror high-resolution single frames is imaged,
Compared to the mechanical structure changeable in the setting of no lens microscopic system light source part, replacement uses three monochromes of red, green, blue in turn
Coherent source is acquired recovery in three times, then is input to the imaging mode that RGB channel carries out summation superposition, dress of the invention
Set it is more compact light, be not necessarily to frequent switching light source, without sampling recovery process, operate simpler convenience.
(2) the present invention is based on the improvement to light source, and three width different color channels can be extracted from single frames sampled images
Image, be not necessarily to toggle lights or filter plate, acquire multiple image and be overlapped again, thus avoid as sample it is mobile caused by
Superimposed image distortion, may be implemented the real-time observation to dynamic sample.
Detailed description of the invention
Fig. 1 is the colored coherent imaging device structural schematic diagram for no mirror microscopic system；
Fig. 2 is schematic diagram of the light source provided by the invention to fiber coupling process；
Fig. 3 is the demodulation crosstalk elimination flow chart that sensor provided by the invention acquires RGB image；
Wherein, 1 it is lighting unit, 2 be optic fiber coupling unit, 3 be filter unit and 4 is detection imaging unit.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
With reference to Fig. 1, on the one hand the embodiment of the present invention provides a kind of colored coherent imaging for no lens microscopic system
Device, comprising: lighting unit 1, optic fiber coupling unit 2, filter unit 3 and detection imaging unit 4；
Lighting unit 1, for exporting three color narrow band light of red, green, blue；Optic fiber coupling unit 2 is used for the red, green, blue
Three color narrowbands are optically coupled into same optical fiber, export complex light；Filter unit 3, for the complex light to the optical fiber output into
After row filter in spatial domain and frequency domain filtering, it is radiated on sample；Detection imaging unit 4 produces after irradiation for receiving sample
Raw initial pictures, and the reduction of demodulation high-resolution is carried out to initial pictures；Wherein, red, green, blue three-color light source line width is in 18nm
To the coherence requirement for the light source between 35nm, meeting lensless system.
Optic fiber coupling unit, including luminous source optical fiber coupling subelement and optical fiber combining coupling subelement；Luminous source optical fiber coupling
Subelement, for three color narrow band light of red, green, blue to be coupled into three optical fiber respectively；Optical fiber combining coupling subelement, is used for three
Three color narrow band lights in root optical fiber are coupled into same multimode fibre, to guarantee the color lighting of coherence；Light source couples arrive
The process of optical fiber is as shown in Fig. 2, the present invention carries out light source couples, the coupling using grin lens (graded index rod-shaped lens)
Coupling efficiency can be improved in mode, reduces coupling loss；Optical fiber combining coupling subelement uses the fiber coupler of 3x1, by three
Three-color light source is coupled into same optical fiber in root optical fiber, realizes secondary color light output.
Filter unit using 50-100um magnitude aperture carry out filter in spatial domain, then using bandwidth be less than or equal to ±
The optical filter of 10nm carries out frequency domain filtering；Aperture and optical filter are close to, and at a distance from imaging detection unit 3-4cm it
Between, cause to collect additional interference on detection imaging unit to avoid because spacing between the two generates additional diffraction light
Pattern.
Since no mirror microscopic system needs to carry out the high-resolution reduction of coherent monochromatic image, image is made to become clear, current
System is monochromation illumination, therefore cmos sensor only receives single pass light, can directly carry out image restoring, and the present invention adopts
With secondary color optical illumination, need to carry out to carry out image high-resolution reduction again after CMOS receives the RGB demodulation of signal, color cross-talk is main
It is to be mixed at the Color Channel of sensor due to sample by the initial pictures that complex light is irradiated, according to test, right
Initial pictures carry out following matrix being used to eliminate color cross-talk in demodulating process；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RbluePoint
Not Biao Shi RGB light practical two-dimensional intensity vector.
Traditional photoelectric coupled device can only incude light intensity, cannot incude color information, need by filter come
Incude color information, referred to as Baeyer filter.Foveon X3 of the present invention using pixel dimension less than or equal to 2.2um is sensed
Device incudes color as detection imaging unit by different depth on a pixel, one layer of induction blue of most surface, the
Two layers can incude green, and third layer induction is red, and three photosensitive layers of Foveon X3 capture RGB color light in different depth,
Then it may insure that RGB color light is all subtracted 100%, the accuracy of coherent imaging can be greatly improved in this way.
The embodiment of the present invention uses the imaging sensor of model AptinaMT9P031 as detection imaging unit, therebetween
Away from having a size of 2.2 μm, effective area is 5.7x 4.28mm, selects three sections of narrow linewidth LED light sources of Thorlabs company as photograph
The end of three light sources need to be only passed through coupler progress as shown in figure 3, the light source and optical fiber have been completed to couple by bright unit
Coupling is emitted after transmitting in the multimode fibre of one section of 1-2cm long, filters by spatial filter, and be radiated on sample,
For light field by receiving detected imaging unit after sample, the light of different wave length is irrelevant each other, thus the light field include it is red,
Green, blue three width holograms, are filtered and are received by different filters respectively, Image Acquisition are carried out at the end PC, to acquisition
After image is demodulated and eliminates crosstalk, individual in-line holographic recovery is carried out to three width holograms, is entered into respective
Color hologram can be obtained in Color Channel.
On the other hand the embodiment of the present invention provides a kind of colored coherent imaging method for no lens microscopic system, wrap
It includes:
(1) three color narrow-band light source of red, green, blue is coupled into same optical fiber, exports complex light；
Specifically, three color narrowband optical linewidth of red, green, blue is in 18nm between 35nm.
(2) it is radiated on sample after the complex light of output being carried out filter in spatial domain and frequency domain filtering, obtains initial pictures；
Specifically, using 50-100um magnitude aperture carry out filter in spatial domain, then using bandwidth be less than or equal to ±
The optical filter of 10nm carries out frequency domain filtering, and aperture and optical filter are close to, in 3-4cm at a distance from initial pictures reception device
Between, additional interference figure is generated to avoid because spacing between the two generates additional diffraction light.
(3) initial pictures are demodulated, obtains three width holograms；
Specifically, during demodulating to initial pictures, use following matrix to eliminate color cross-talk；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RbluePoint
Not Biao Shi RGB light practical two-dimensional intensity vector.
(4) reduction of image high-resolution is carried out to three obtained width holograms, obtains color hologram.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Claims (10)
1. a kind of colored coherent imaging device for no lens microscopic system characterized by comprising lighting unit (1), light
Fine coupling unit (2), filter unit (3) and detection imaging unit (4)；
The lighting unit (1), for exporting three color narrow band light of red, green, blue；
The optic fiber coupling unit (2) exports compound for the three color narrowband of red, green, blue to be optically coupled into same optical fiber
Light；
The filter unit (3), after carrying out filter in spatial domain and frequency domain filtering for the complex light to the optical fiber output, irradiation
On sample；
The detection imaging unit (4), the initial pictures generated after irradiation for receiving the sample, and to described initial
Image carries out the reduction of demodulation high-resolution.
2. a kind of colored coherent imaging device for no lens microscopic system according to claim 1, which is characterized in that
The three color narrowband optical linewidth of red, green, blue is in 18nm between 35nm.
3. a kind of colored coherent imaging device for no lens microscopic system according to claim 1 or 2, feature exist
In, the optic fiber coupling unit, including luminous source optical fiber coupling subelement and optical fiber combining coupling subelement；
The luminous source optical fiber couples subelement, for three color narrow band light of red, green, blue to be coupled into three optical fiber respectively；
The optical fiber combining coupling subelement, for three color narrow band lights in three optical fiber to be coupled into same optical fiber.
4. a kind of colored coherent imaging device for no lens microscopic system according to claim 3, which is characterized in that
The luminous source optical fiber coupling subelement is graded index rod-shaped lens；The optical fiber that the optical fiber combining coupling subelement is 3x1
Coupler.
5. a kind of colored coherent imaging device for no lens microscopic system according to claim 1-4,
It is characterized in that, the filter unit carries out filter in spatial domain using the aperture of 50-100um magnitude, is less than or equal to using bandwidth
The optical filter of ± 10nm carries out frequency domain filtering, and the aperture and optical filter are close to, and at a distance from the imaging detection unit
Between 3-5cm.
6. a kind of colored coherent imaging device for no lens microscopic system according to claim 1-5,
It is characterized in that, during the imaging detection unit demodulates initial pictures, uses following matrix to eliminate color string
It disturbs；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RblueTable respectively
Show the practical two-dimensional intensity vector of RGB light.
7. a kind of colored coherent imaging device for no lens microscopic system according to claim 6, which is characterized in that
The detection imaging unit is Foveon X3 sensor, and its pixel dimension is less than or equal to 2.2um.
8. a kind of colored coherent imaging method for no lens microscopic system characterized by comprising
(1) three color narrow-band light source of red, green, blue is coupled into same optical fiber, exports complex light；
(2) it is radiated on sample after the complex light of output being carried out filter in spatial domain and frequency domain filtering, obtains initial pictures；
(3) initial pictures are demodulated, obtains three width holograms；
(4) reduction of image high-resolution is carried out to three obtained width holograms, obtains color hologram.
9. a kind of colored coherent imaging method for no lens microscopic system according to claim 8, which is characterized in that
The three color narrowband optical linewidth of red, green, blue is in 18nm between 35nm.
10. a kind of colored coherent imaging method for no lens microscopic system according to claim 9, feature exist
In using following matrix to eliminate color cross-talk during demodulating to initial pictures；
Wherein, Mred、Mgreen、MblueRespectively indicate the two-dimensional intensity vector of RGB light measurement, Rred、Rgreen、RblueTable respectively
Show the practical two-dimensional intensity vector of RGB light.
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