CN110243873B - Multifunctional cell sensor system and measuring method thereof - Google Patents
Multifunctional cell sensor system and measuring method thereof Download PDFInfo
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- CN110243873B CN110243873B CN201910585810.0A CN201910585810A CN110243873B CN 110243873 B CN110243873 B CN 110243873B CN 201910585810 A CN201910585810 A CN 201910585810A CN 110243873 B CN110243873 B CN 110243873B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
Abstract
The present disclosure relates to a multifunctional cell sensor system and a measurement method thereof. The system comprises: the switching device comprises a first electrode, a second electrode and a switching part, wherein the first electrode comprises a first interdigital electrode component and a second interdigital electrode component which form an interdigital electrode; the switching part is connected with the first interdigital electrode assembly and the second interdigital electrode assembly, or connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly. The switching of the connection between the electrodes is realized through the switching component, so that the functional cell sensor system can be used for measuring the impedance value of the cell and the transmembrane resistance value of the cell, and can carry out real-time, quantitative, nondestructive, rapid and convenient detection on the adhesion, migration and connection of the cell.
Description
Technical Field
The disclosure relates to the technical field of biomedical treatment, in particular to a multifunctional cell sensor system and a measuring method thereof.
Background
A cell sensor is a type of biosensor that can detect biochemical actions directly by living cells and convert them into electrical signals by a transducer. In particular, cell sensors employ immobilized living cells as sensitive elements in combination with physical or chemical transducers. The cell impedance sensor system is a technical platform for dynamic research of cell behavior, and can research the changes of the morphology, the position, the number and the like of growing cells in real time, quantitatively, without damage or intervention. It has three main characteristics: the non-invasive cell dynamic research platform does not need staining, marks to quantitatively measure in real time, and obtains data experiment results in real time so as to have high repeatability.
The cell impedance measuring system based on the interdigital electrode is also a cell sensor, can perform nondestructive detection, and more importantly can detect changes of adhesion, growth, migration and the like of cells in real time. In particular, the tight junctions associated with the brain microvascular endothelial cells that make up the blood-brain barrier play an important role in their selective passage.
The traditional technology for preparing the interdigital electrode is magnetron sputtering, electron beam evaporation coating and other technologies, and then the steps of photoetching, corrosion and the like are carried out, so that special equipment and a complex and expensive process are needed, and the period is long.
In addition, the existing cell measurement is based on a Transwell chamber, but the Transwell chamber is very inconvenient for measuring cell adhesion and transmembrane resistance, and the observation of cell adhesion depends on empirical data or is taken off for scanning electron microscope observation, which is a destructive observation means and cannot realize real-time monitoring.
Disclosure of Invention
In view of the above, the present disclosure provides an interdigital electrode preparation method, a multifunctional cell sensor system and a measurement method thereof.
According to an aspect of the present disclosure, there is provided a multifunctional cell sensor system including: a first electrode, a second electrode and a switching member,
the first electrode comprises a first interdigital electrode assembly and a second interdigital electrode assembly, and the first interdigital electrode assembly and the second interdigital electrode assembly form an interdigital electrode;
the switching part is connected with the first interdigital electrode assembly and the second interdigital electrode assembly, or connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly.
In one possible implementation, when the switching component is connected with the first interdigital electrode assembly and the second interdigital electrode assembly, the multifunctional cell sensor system is used for measuring the impedance value of the cell;
the multi-functional cell sensor system is configured to measure a transmembrane resistance value of a cell when the switching member is connected to the second electrode and one of the first interdigitated electrode assembly and the second interdigitated electrode assembly.
In one possible implementation, the system further includes: the data acquisition device and the signal processing device;
the data acquisition device is respectively connected with the signal processing device and the input control end of the switching part; the signal processing device is connected to the first electrode and/or the second electrode through an output end of the switching component.
In a possible implementation manner, the data acquisition device provides a switching control instruction to an input control end of the switching component;
the switching part is switched according to the switching control instruction so as to communicate the signal processing device with the first interdigital electrode assembly and the second interdigital electrode assembly or communicate the signal processing device with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
and the signal processing device sends the obtained measurement data to the data acquisition device.
In one possible implementation, the first electrode and the second electrode are multiple, and one first electrode and one second electrode are combined into one electrode pair.
In one possible implementation manner, the switching component is multiple, and the signal processing device comprises multiple channels;
each channel is connected to the first electrode and/or the second electrode of the one electrode pair through the output of one switching member.
In one possible implementation, the system further comprises Transwell cells, and each of the electrode pairs is disposed in one of the Transwell cells.
In a possible implementation manner, the system further comprises a terminal device, wherein the terminal device is connected with the data acquisition device,
the terminal equipment provides a switching control instruction to the switching part through the data acquisition device and receives measurement data from the data acquisition device.
In a possible implementation manner, the interdigital electrode is formed by printing carbon nanotube microfilaments according to an interdigital electrode pattern by using a high-voltage electrostatic spinning direct writing technology.
According to another aspect of the present disclosure, there is provided a measurement method based on the above multifunctional cell sensor system, including:
controlling the switching part to be connected with the first interdigital electrode assembly and the second interdigital electrode assembly so as to measure the impedance value of the cell; or
And controlling the switching part to be connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly so as to measure the transmembrane resistance value of the cell.
In one possible implementation, the method further includes:
the terminal equipment provides a switching control instruction for the switching part through a data acquisition device, wherein the switching control instruction is used for controlling the switching part to be connected with the first interdigital electrode assembly and the second interdigital electrode assembly or controlling the switching part to be connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
the signal processing device processes the received electric signals to obtain measurement data and sends the measurement data to the data acquisition device;
the data acquisition device sends the received measurement data to the terminal equipment;
and the terminal equipment displays the impedance value of the cell or the transmembrane resistance value of the cell according to the measurement data.
The switching of the connection between the electrodes is realized through the switching component, so that the functional cell sensor system can be used for measuring the impedance value of the cell and the transmembrane resistance value of the cell, and can carry out real-time, quantitative, nondestructive, rapid and convenient detection on the adhesion, migration and connection of the cell.
Other features and aspects of the present disclosure will become apparent from the following detailed description of exemplary embodiments, which proceeds with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate exemplary embodiments, features, and aspects of the disclosure and, together with the description, serve to explain the principles of the disclosure.
Fig. 1 shows a block diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure.
Fig. 2 shows a schematic structural diagram of an interdigital electrode in accordance with an embodiment of the present disclosure.
FIG. 3a shows a schematic diagram of a practical application of a multifunctional cell sensor system according to an embodiment of the present disclosure.
Fig. 3b shows a schematic diagram of a connection structure of a switching member and an electrode pair according to an embodiment of the present disclosure.
FIG. 4 shows a schematic diagram of cell impedance measurement according to an embodiment of the present disclosure.
Fig. 5 shows a schematic structural diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure.
Fig. 6 shows a schematic structural diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure.
Fig. 7 shows a flowchart of a measurement method based on the multifunctional cell sensor system according to an embodiment of the present disclosure.
Detailed Description
Various exemplary embodiments, features and aspects of the present disclosure will be described in detail below with reference to the accompanying drawings. In the drawings, like reference numbers can indicate functionally identical or similar elements. While the various aspects of the embodiments are presented in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present disclosure. It will be understood by those skilled in the art that the present disclosure may be practiced without some of these specific details. In some instances, methods, means, elements and circuits that are well known to those skilled in the art have not been described in detail so as not to obscure the present disclosure.
Fig. 1 shows a block diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure. The system can be used for detecting experiments such as blood brain barrier model simulation, vascular permeability, tumor diffusion and migration. As shown in fig. 1, the multifunctional cell sensor system may include: a first electrode 105, a second electrode 106 and a switching member 104,
the first electrode 105 may include a first interdigital electrode assembly 1051 and a second interdigital electrode assembly 1052, and the first interdigital electrode assembly 1051 and the second interdigital electrode assembly 1052 may constitute an interdigital electrode, which may be an electrode having a periodic pattern in its surface, such as a finger or comb, as shown in fig. 2. For example, first interdigital electrode assembly 1051 and second interdigital electrode assembly 1052 can be two parts in which the insides of the interdigital electrodes are not connected to each other. The interdigital electrodes may be printed on a substrate, which may be a glass substrate or a flexible substrate, which is not limited in this disclosure. In one example, as shown in FIG. 3a, a Transwell (cell migration invasion assay) chamber is an assay device having two chambers, one above the other and one between two porous filters. The interdigitated electrodes 105 may be printed on top of the porous filter membrane. Wherein the Transwell cell may include an interdigital electrode 105, a second electrode 106, a top cover plate 109, a lead-out wire 108, an upper cell 201, and a lower cell 202, and the interdigital electrode 105 may be connected to the switching member 104 through the lead-out wire 108. At the time of measurement, an experimental cell 107 may be planted on the interdigital electrode 105, and an experiment may be performed.
The switching member 104 can be connected to the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052, or to the second electrode 106 and one of the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052.
The switching component may enable switching of electrical circuit connections, for example, from connection with the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052, to connection with the second electrode 106 and one of the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052 (i.e., connection with the second electrode 106 and the first interdigitated electrode assembly 1051, or connection with the second electrode 106 and the second interdigitated electrode assembly 1052); alternatively, the connection to the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052 may be switched from the connection to the second electrode 106 and one of the first interdigitated electrode assembly 1051 and the second interdigitated electrode assembly 1052. Wherein the switching means may perform the switching according to a received switching control instruction.
The switching means may include a device capable of performing circuit opening and closing (circuit switching), for example, an electromagnetic relay or the like, and the switching may be performed by opening and closing of contacts of the electromagnetic relay.
In an example, taking an electromagnetic relay as a switching component as an example, as shown in fig. 3b, one end of the electromagnetic relay may be connected to the second interdigital electrode assembly 1052, and the other end 1041 of the electromagnetic relay may be used as a switch to switch between the first interdigital electrode assembly 1051 and the second electrode 106, and the first interdigital electrode assembly 1051 or the second electrode 106 may be connected to implement a switching function of the circuit connection of the switching component. The other end 1041 of the electromagnetic relay can realize the switching between the connection with the first interdigital electrode assembly 1051 or the connection with the second electrode 106 according to the switching control command received by the input control end of the electromagnetic relay. Correspondingly, two outgoing lines 108 in fig. 3a may be provided, one connected to the first interdigital electrode assembly 1051 and the other connected to the second interdigital electrode assembly 1052, and the outgoing lines may be respectively connected to the ends of the electromagnetic relay, so as to connect the ends of the electromagnetic relay to the first interdigital electrode assembly and/or the second interdigital electrode assembly.
It should be understood by those skilled in the art that fig. 3b is only an exemplary connection manner between the switching component and the electrode pair, and the disclosure is not limited to the specific form and connection manner of the switching component, for example, one end of the electromagnetic relay may be connected to the first interdigital electrode assembly 1051, and the other end of the electromagnetic relay may be used as a switch to switch between the second interdigital electrode assembly 1052 and the second electrode 106, as long as the above-mentioned required switching manner can be achieved.
The switching of the connection is realized through the switching part, so that the multifunctional cell sensor system can conveniently detect different cell activity state information in real time.
In a possible implementation manner, the interdigital electrode can be formed by printing carbon nanotube micro-wires according to an interdigital electrode pattern by using a high-voltage electrostatic spinning direct writing technology.
The interdigital electrode pattern can be designed in advance according to actual requirements, and the interdigital electrode pattern can be used as a basis for preparing an interdigital electrode.
The high-voltage electrostatic spinning direct-writing technology is a process for carrying out jet spinning on polymer solution or melt in a strong electric field. Under the action of the high-voltage electric field, the liquid drop at the needle head changes from a spherical shape to a conical shape (namely a Taylor cone) and a fiber filament is obtained by extending from the tip of the cone. This way, polymer filaments of nanometer-scale diameter can be produced. The traditional electrospinning produces disordered, irregularly arranged microfilaments. However, the high-voltage electrostatic spinning direct writing technology can accurately control the fiber deposition sites. The technical principle is that the spinning jet flow is controlled to be in a stable motion state by reducing the spinning distance (0.2mm-10mm) and the spinning voltage (about 1-4kV), and the accurate deposition of the single spinning jet flow is realized.
The carbon nanotube, also called buckytubes, is a new material with a special structure, the radial dimension is nanometer magnitude, the axial dimension is micrometer magnitude, and the carbon nanotube has good conductivity.
The interdigital electrode preparation equipment can obtain the interdigital electrode pattern, the high-voltage electrostatic spinning direct writing technology is utilized to print and form the carbon nano tube micro-wires according to the interdigital electrode pattern (design pattern), and the printed and formed shape pattern forms the interdigital electrode. Wherein the carbon nanotube microfilament may be a carbon nanotube microfilament.
When the interdigital electrode is prepared, the carbon nanotube-containing microfilament is printed and formed according to the designed interdigital electrode pattern by using a high-voltage electrostatic spinning direct writing technology, according to the interdigital electrode preparation method disclosed by the embodiment of the disclosure, various complex electrode patterns can be quickly printed according to actual requirements, the manufacturing period and difficulty of the interdigital electrode are greatly reduced, and therefore the detection of the activity state information of cells can be facilitated.
In one possible implementation, the multifunctional cell sensor system may be configured to measure an impedance value of a cell when the switching member is connected with the first interdigitated electrode assembly and the second interdigitated electrode assembly; the impedance value of the cell in different activity states is measured between the first interdigital electrode assembly and the second interdigital electrode assembly in different activity states. The different activity states of the cells may include adhesion, growth, etc. of the cells. The impedance value can be used to indicate cell adhesion, production.
The multi-functional cell sensor system can be used to measure a transmembrane resistance value of a cell when the switching member is connected to the second electrode and one of the first interdigitated electrode assembly and the second interdigitated electrode assembly. The transmembrane resistance value can be used to indicate the connectivity of the cell.
When cells with adherent properties are attached to the electrodes, measuring the impedance between the electrodes provides important information about the status of the activity of the cells on the electrodes, the principle of which is shown in fig. 4. The larger the area of the electrode surface that is shielded, the larger the impedance that is measured. Therefore, it can be used to analyze the adhesion process of cells and the number of cells adhered. After the cells are planted on the surface of the interdigital electrode, the cells can gradually stretch, migrate and proliferate, and the cells can contact and adhere to the surface of the electrode, so that the impedance is increased. In addition, the electrical impedance may also vary depending on whether the cells are tightly attached.
The switching component can be connected with the first interdigital electrode component and the second interdigital electrode component and disconnected with the second electrode, so that the multifunctional cell sensor system is used for measuring the impedance value of the cell, namely the first interdigital electrode component and the second interdigital electrode component are involved in work when the impedance value of the cell is measured, and the resistance value between the first interdigital electrode component and the second interdigital electrode component is measured; the switching means may be connected to the second electrode and to one of said first and second interdigitated electrode assemblies, such that the multifunctional cell sensor system is adapted to measure the transmembrane resistance of a cell, i.e. the resistance between the second electrode and said first interdigitated electrode assembly or the resistance between the second electrode and the second interdigitated electrode assembly, the second electrode and said one of the first and second interdigitated electrode assemblies are involved in the measurement operation when measuring the transmembrane resistance of a cell.
The switching of the connection is realized through the switching component, so that the functional cell sensor system can be used for measuring the impedance value of the cell and the transmembrane resistance value of the cell, and can carry out real-time, quantitative, nondestructive, rapid and convenient detection on the adhesion, migration and connection of the cell.
Fig. 5 shows a schematic structural diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure. As shown in fig. 5, in one possible implementation, the system may further include: a data acquisition device 102 and a signal processing device 103;
the data acquisition device 102 is respectively connected with the signal processing device 103 and the input control end of the switching component 104; the signal processing means 103 may be connected to the first electrode and/or the second electrode through an output of the switching member 104.
Wherein the signal processing device may be an electrochemical workstation or the like, and the signal processing device may obtain the required measurement data based on the received electrical signals based on the related art. The electrical signals received by the signal processing means may be from the first and second interdigitated electrode assemblies in the first electrode, or may be from one of the first and second interdigitated electrode assemblies, and the second electrode. For example, the signal processing device may obtain the impedance value of the cell from the electric signals from the first interdigital electrode assembly and the second interdigital electrode assembly by an alternating current impedance method, or the signal processing device may also obtain the transmembrane resistance value of the cell from the electric signals from one of the first interdigital electrode assembly and the second interdigital electrode assembly, and the second electrode. The signal processing device can be directly used for measuring the steady-state current on the ultramicroelectrode, and has a wide dynamic range, so that the measurement is sensitive.
In a possible implementation manner, the data acquisition device may provide a switching control instruction to the input control end of the switching component;
the switching part can be switched according to the switching control instruction to communicate the signal processing device with the first interdigital electrode assembly and the second interdigital electrode assembly or communicate the signal processing device with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
the signal processing means may send the obtained measurement data to the data acquisition means.
As shown in fig. 5, in one possible implementation, the system may further include: a terminal device connectable with the data acquisition apparatus,
the terminal device may provide a switching control instruction to the switching part through the data acquisition device, and receive measurement data from the data acquisition device.
Wherein the measurement data may comprise an impedance value of the cell or a transmembrane resistance value of the cell. The terminal equipment can comprise a computer, a smart phone and the like.
In one example, the data acquisition device may be disposed in the terminal device, for example, may be a data acquisition card installed in a PCI slot of the terminal device.
The terminal equipment provides a switching control instruction for the switching part to control the connection of the switching part and the first electrode and/or the second electrode, so that the measurement of different activity state information of the cell is realized, the measurement of the cell can be set by a user through the terminal equipment, and the convenience and the flexibility of the measurement are improved.
Those skilled in the art should understand that the present disclosure does not limit the generation manner of the switching control instruction, for example, the switching control instruction may be set by the terminal device, and may also be preset in the data acquisition device, etc. The specific content of the switching control command can be set according to the needs, and the disclosure does not limit this.
It will be understood by those skilled in the art that the connections between the switching member 104 and the first and second electrodes 105 and 106 in fig. 5 and fig. 6 below are schematic, and the specific connection manner is shown in fig. 3b (and the enlarged partial view in fig. 5), for example (but not limited thereto).
Fig. 6 shows a schematic structural diagram of a multifunctional cell sensor system according to an embodiment of the present disclosure. As shown in fig. 6, in a possible implementation manner, the first electrode and the second electrode may be multiple, such as multiple first electrodes 105 and multiple first electrodes 106 in fig. 6.
Wherein one first electrode and one second electrode may be combined into one electrode pair. The multifunctional cell sensor system may further comprise Transwell chambers, and each electrode pair may be disposed within one of the Transwell chambers, as shown in fig. 6. The signal processing means may perform measurements on a plurality of cells in parallel. See above for the Transwell chamber.
As shown in fig. 6, in one possible implementation, the switching component 104 may be multiple, and the signal processing apparatus may include multiple channels; each channel may be connected to the first electrode and/or the second electrode of the one electrode pair via the output of one switching member, i.e. multiple channels of the signal processing means may process measurements of multiple Transwell cells in parallel. For example, each channel may be connected to the first interdigitated electrode assembly and the second interdigitated electrode assembly of the first electrode in one electrode pair or to one of the first interdigitated electrode assembly and the second interdigitated electrode assembly in one electrode pair and the second electrode through the output of one switching element. Wherein each channel is correspondingly connected with one switching component in the switching components and one electrode pair so as to realize the measurement of one chamber.
The plurality of Transwell chambers may also be independent in measurement mode, which may include measuring an impedance value of a cell, measuring a transmembrane resistance value of a cell, etc., wherein one or more of the Transwell chambers may be used to measure an impedance value of a cell and one or more other of the Transwell chambers may be used to measure a transmembrane resistance value of a cell. The measurement modes of the plurality of Transwell chambers can be independently controlled by the terminal equipment so as to realize independent switching of the measurement modes.
The signal processing device is arranged into multiple channels, so that the purpose of measuring multiple chambers in parallel is achieved, the measurement efficiency is improved, each chamber can be independently measured, and the impedance value of the cell and the transmembrane resistance value of the cell can be simultaneously measured.
Fig. 7 shows a flowchart of a measurement method based on the multifunctional cell sensor system according to an embodiment of the present disclosure. As shown in fig. 7, the measurement method may include:
step S21, controlling the switching component to connect with the first interdigital electrode assembly and the second interdigital electrode assembly to measure the impedance value of the cell; or
And step S22, controlling the switching component to be connected with the second electrode and one of the first interdigital electrode component and the second interdigital electrode component so as to measure the transmembrane resistance value of the cell.
The connection is switched by the switching component, so that the multifunctional cell sensor system can measure the impedance value of the cell and the transmembrane resistance value of the cell, avoid destructive measurement means of the Transwell chamber and realize real-time monitoring.
In one possible implementation, the method may further include:
the terminal equipment provides a switching control instruction for the switching part through a data acquisition device, wherein the switching control instruction is used for controlling the switching part to be connected with the first interdigital electrode assembly and the second interdigital electrode assembly or controlling the switching part to be connected with the first electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
the signal processing device processes the received electric signals to obtain measurement data and sends the measurement data to the data acquisition device; wherein the measurement data may comprise an impedance value of the cell or a transmembrane resistance value of the cell.
The data acquisition device can send the received measurement data to the terminal equipment;
the terminal device may display the impedance value of the cell or the transmembrane resistance value of the cell according to the measurement data to present the measurement data to a user of the terminal device.
The switching control instruction is provided through the terminal equipment, and the impedance value of the cell or the transmembrane resistance value of the cell is displayed, so that a user of the terminal equipment can conveniently measure required measurement data and timely acquire the measurement data.
The electrical signal may include a voltage signal or a current signal, among others.
Taking the multifunctional cell sensor system shown in fig. 5 as an example, in use, the terminal device 101 may send a switching control instruction to the input control terminal of the switching unit 104 through the data acquisition device 102, so that the switching unit 104 connects the first interdigital electrode assembly and the second interdigital electrode assembly, the signal processing device 103 obtains an impedance value of the measured cell according to a signal obtained by the switching unit 104, and sends the impedance value to the data acquisition device 104, and the data acquisition device 104 sends the impedance value to the terminal device 101 for further data processing, storage, display, and the like.
The terminal device 101 may also send a switching control instruction to the input control end of the switching unit 104 through the data acquisition device 102, so that the switching unit 104 is connected to the second electrode and the first interdigital electrode assembly, or the second electrode and the second interdigital electrode assembly, the signal processing device 103 obtains a transmembrane resistance value of the measured cell according to the signal obtained by the switching unit 104, and sends the transmembrane resistance value to the data acquisition device 104, and the data acquisition device 104 sends the transmembrane resistance value to the terminal device 101 for further data processing, storage, display, and the like.
For exemplary explanations of the process parts, see above, this is not repeated here.
Having described embodiments of the present disclosure, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen in order to best explain the principles of the embodiments, the practical application, or improvements made to the technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A multifunctional cell sensor system, comprising: a first electrode, a second electrode and a switching member,
the first electrode comprises a first interdigital electrode assembly and a second interdigital electrode assembly, and the first interdigital electrode assembly and the second interdigital electrode assembly form an interdigital electrode;
the switching part is connected with the first interdigital electrode assembly and the second interdigital electrode assembly or connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
when the switching part is connected with the first interdigital electrode assembly and the second interdigital electrode assembly, the multifunctional cell sensor system is used for measuring the impedance value of the cell;
the multi-functional cell sensor system is configured to measure a transmembrane resistance value of a cell when the switching member is connected to the second electrode and one of the first interdigitated electrode assembly and the second interdigitated electrode assembly.
2. The system of claim 1, further comprising: the data acquisition device and the signal processing device;
the data acquisition device is respectively connected with the signal processing device and the input control end of the switching part; the signal processing device is connected to the first electrode and/or the second electrode through an output end of the switching component.
3. The system of claim 2, wherein the data acquisition device provides switching control instructions to the input control end of the switching component;
the switching part is switched according to the switching control instruction so as to communicate the signal processing device with the first interdigital electrode assembly and the second interdigital electrode assembly or communicate the signal processing device with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
and the signal processing device sends the obtained measurement data to the data acquisition device.
4. The system of claim 3, wherein the first and second electrodes are a plurality of electrodes, and wherein a first electrode and a second electrode are combined into an electrode pair.
5. The system of claim 4, wherein the switching member is plural, and the signal processing device includes plural channels;
each channel is connected to the first electrode and/or the second electrode of the one electrode pair through the output of one switching member.
6. The system of claim 4 or 5, further comprising Transwell cells, wherein each of the electrode pairs is disposed in one of the Transwell cells.
7. The system according to claim 2 or 3, further comprising a terminal device connected to the data acquisition device,
the terminal equipment provides a switching control instruction to the switching part through the data acquisition device and receives measurement data from the data acquisition device.
8. The system according to any one of claims 1 to 5, wherein the interdigital electrode is formed by printing carbon nanotube microfilaments according to an interdigital electrode pattern by using a high-voltage electrospinning direct writing technology.
9. A measurement method based on the multifunctional cell sensor system of any one of claims 1 to 8, comprising:
controlling the switching part to be connected with the first interdigital electrode assembly and the second interdigital electrode assembly so as to measure the impedance value of the cell; or
And controlling the switching part to be connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly so as to measure the transmembrane resistance value of the cell.
10. The measurement method according to claim 9, characterized in that the method further comprises:
the terminal equipment provides a switching control instruction for the switching part through a data acquisition device, wherein the switching control instruction is used for controlling the switching part to be connected with the first interdigital electrode assembly and the second interdigital electrode assembly or controlling the switching part to be connected with the second electrode and one of the first interdigital electrode assembly and the second interdigital electrode assembly;
the signal processing device processes the received electric signals to obtain measurement data and sends the measurement data to the data acquisition device;
the data acquisition device sends the received measurement data to the terminal equipment;
and the terminal equipment displays the impedance value of the cell or the transmembrane resistance value of the cell according to the measurement data.
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