CN110235988A - A kind of antibacterial peptide type feed for pet and preparation method thereof - Google Patents
A kind of antibacterial peptide type feed for pet and preparation method thereof Download PDFInfo
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- CN110235988A CN110235988A CN201910650902.2A CN201910650902A CN110235988A CN 110235988 A CN110235988 A CN 110235988A CN 201910650902 A CN201910650902 A CN 201910650902A CN 110235988 A CN110235988 A CN 110235988A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The present invention discloses a kind of antibacterial peptide type feed for pet and preparation method thereof, and the high yield antibacterial peptide bacterial strain after screening is carried out fermented and cultured；Semi-finished product pet basal feed is prepared, and carries out extruding granulation；By grease even application on pet basal feed swelling granular surface；Fermentation liquid is sprayed at by oil spout treated pet basal feed swelling granular surface, antibacterial peptide type feed for pet is made；Cool down after the granulation of feed for pet extruding, add antibacterial peptide components, can avoid destruction of the high temperature to antibacterial peptide structure and property, increases the concentration of effective component in feed；The present invention uses antibacterial peptide as feed for pet raw material, and broad-spectrum antibacterial antiviral, adjusting is immune, promotes the characteristic of growth that pet quality of life will be made to greatly improve, while reducing the lethal probability of its illness.
Description
Technical field
The present invention relates to feed for pet preparation and processing technique fields, and in particular to a kind of antibacterial peptide type feed for pet and its
Preparation method.
Background technique
As income level improves, social senilization's aggravation, demand of the Chinese to pet constantly increases.In nearly 1,400,000,000 people
The China of mouth, there are about 90,000,000 for pet cat and dog.It is reported that China has been the second-biggest-in-the-world pet city for being only second to the U.S.
, the pet upsurge that becomes more and more hotter has expedited the emergence of the development of pets economy industrial chain.Wherein, rigidity of the pet food as raising pet
Demand, accounting share highest, reaches 35.6 in pet industry.Under the background that market scale constantly increases, pet owner
It is more fastidious, in terms of selecting pet food, gradually natural safety is placed above the other things.
Contain various food additives more in common feed for pet currently on the market, is taken in for a long time so as to cause pet each
Kind food additives, to cause the enterogastric diseases of pet；Simultaneously because antibiotic is widely used, body drug resistance and medicine
Object residual makes pet normal intestinal flora lack of proper care, and so that pet is easy to produce the intestines problems such as constipation, indigestion, enterogastritis, gives
The health of pet brings harm.
Pollute many factors such as more viral life environment due to bad feeding manner and in more bacterium influences more, pet constitution
Difference, premunition are weak, when by cause pathogeny imcrobe infection, are easy to cause the symptom of pet illness, especially diarrhea more common.
Though antibiotic has the characteristics that rapidly and efficiently when treating pet diarrhea, long-term a large amount of uses will cause antibiotic residue dirt
Dye, " super drug-fast bacteria " generate, to break the intracorporal homeostasis of pet, cause pet autogenous infection.Occurred in recent years
Some Novel pet preparations, but its shared feature be it is expensive, administration route is complicated, keep the main cost of pet excessively high, cultivation at
This sharp increase, therefore new formulation Difficulty.
Antibacterial peptide (AMP s) is a kind of prokaryotes and eucaryote can generate, is widely distributed and miscellaneous,
Polypeptides matter with broad spectrum antibiotic activity is made of 40-50 amino acid residue mostly, it is to bacterium, fungi, primary dynamic
Object, cancer cell, virus etc. have apparent inhibiting effect.Studies have shown that adding antibacterial peptide in animal feed is able to suppress disease
Bacterium breeding, improves microbial population of animal intestinal tract structure, improves animal quality of life, reduces illness rate.
Summary of the invention
It is of the existing technology in order to solve the problems, such as, it is an object of that present invention to provide a kind of antibacterial peptide type feed for pet and its
Preparation method, the feed for pet after adding antibacterial peptide, can significantly increase the immunity of animal, reduce its illness lethality, improve
Bacterial community in pet gastrointestinal tract promotes its quality of life.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of antibacterial peptide type feed for pet, by adding in pet basal feed by fermentation of bacillus subtilis culture
Antibacterial peptide fermentation liquid is made.
A kind of preparation method of antibacterial peptide type feed for pet, includes the following steps:
(1) fermentation process: bacillus subtilis strain is subjected to fermented and cultured, obtains antibacterial peptide fermentation liquid, is placed in sterile
It is spare under environment；
(2) pelletization treatment: preparation pet basal feed, and carry out extruding granulation；
(3) drying and processing: pet basal feed particle is dried；
(4) first time cooling treatment: by the pet basal feed swelling granular cooling down of drying to 50-60 DEG C；
(5) oil spout is handled: by grease even application on pet basal feed swelling granular surface, being completed material after operating
Place cooling；
(6) whitewashing is handled: it is swollen that fermentation liquid obtained in step (1) is sprayed at the pet basal feed by oil spout processing
Change particle surface；
(7) second of cooling treatment: it pet basal feed swelling granular will be cooled to room temperature, be made after whitewashing is handled
Antibacterial peptide type feed for pet.
Further, manufactured antibacterial peptide fermentation liquid potency is not less than 4600 active units/milliliter in the step (1).
Further, grease used is vegetable oil or fish oil in the step (5).
Further, the sprinkling ratio of step (5) grease is to add 0.1 kilogram of grease in 1 kilogram of basal feed.
Further, spraying operation is carried out again after grease used being heated to 50-60 DEG C in the step (5).
Further, in the ratio sprinkling fermentation liquid for adding 0.5 liter of fermentation liquid in 1 kilogram of basal feed in the step (6).
Further, the type of cooling is that aseptic condition leeward is dry and cold but in the step (7).
Further, it is lower than made antibacterial peptide type feed for pet water content through air-drying cooling in the step (7)
14%.
Further, pet basal feed is subjected to extruding granulation at 120-150 DEG C in the step (2).
Antibacterial peptide type feed for pet of the present invention and preparation method thereof prepares withered grass bud used in antibacterial peptide type feed for pet
Spore bacillus antibacterial peptide can effectively inhibit the infection of Escherichia coli, staphylococcus aureus and salmonella to encroach on, in pet enteron aisle
Middle foundation is biological " defence line ", reduces poisonous and harmful substance and enters in pet body, maintains intestinal eubiosis, and body can be promoted to produce
Raw antibacterial substance, enhances immune function, improves the disease resistance of pet, significantly improve the symptom of diarrhea of pet.Meanwhile it is anti-
Immune defense effect provided by bacterium peptide, can be such that pet illness rate is greatly reduced, and promote pet quality of life.Furthermore it is verified
It was found that the effect of blast hair color can also be reached using the pet that antibacterial peptide type feed for pet prepared by the present invention is fed.
A small number of zooperies prove that this antibacterial peptide type feed has the function of improving pet hair color.
Compared with the existing technology, it advantage of the present invention and has the beneficial effect that:
(1) antibacterial peptide of antibacterial peptide type feed for pet provided by the invention, addition is produced from fermentation of bacillus subtilis
Object, and the feed addictive type that bacillus subtilis allows for national feed management agencies dictate, therefore market access difficulty
It reduces.
(2) antibacterial peptide type feed for pet provided by the invention, because antibacterial peptide is innovatively added, therefore it can have been killed
The evil microorganisms such as bacterium or fungi can supplement normal flora and animal beneficial in animal body or in enteron aisle from many aspects and normally give birth to
Nutrition needed for managing.It can also maintain or adjust the microecological balance at multiple positions in animal body, inhibit the growth of pathogen, increase
Strong autoimmune function is conducive to sorption enhanced and the metabolism of nutriment.Its metabolism can generate a variety of digestive ferments, can be improved
The absorption of feed conversion rate and animal itself to nutriment, to reach prevention and control disease, treatment disease, improve efficiency of feed utilization
With the target of production performance.
(3) antibacterial peptide type feed for pet provided by the invention, because important source material antibacterial peptide mechanism of action is different from antibiotic,
Its Antibacterial Mechanism has diversity, does not generate drug resistance, therefore feed provided by the invention does not have toxic side effect, without drug resistance
Property, noresidue, effect is lasting and significant.
(4) it is Celsius to be first preheating to 50-60 in oil spout processing by antibacterial peptide type feed for pet provided by the invention for grease
Degree is sprayed again, is at this temperature sprayed grease to the surface of expanded pellet feed, is enabled grease effectively to extruding
Pellet is not permeated, and because the freezing point temperature of grease is higher, is not easily led to grease at this time and is only wrapped in swelling granular feeding
The case where expecting surface, to because of the friction generated between expanded pellet feed and be touched during not easily leading to subsequent packages, storage and transportation
It hits so that the phenomenon that grease of expanded pellet diet particle surface is stripped.
Antibacterial peptide type feed for pet provided by the invention will carry out again whitewashing processing after material cooling after oil spout processing,
It not will lead to because temperature of charge reduces therefore the problem of antibacterial peptide inactivates, ensured the total amount of antibacterial peptide in finished product feedstuff.
Detailed description of the invention
Fig. 1 is process flow chart of the invention
Specific embodiment
Present invention is further described in detail combined with specific embodiments below, but not as a limitation of the invention.
The present invention provides a kind of antibacterial peptide type feed for pet and preparation method thereof, below by specific embodiment to this hair
It is bright to be described in more detail, so that reader better understands the present invention, but following embodiments be not intended to limit it is of the invention
Range.
Embodiment 1
Fig. 1, antibacterial peptide type feed for pet preparation method of the present invention are please referred to, process flow includes:
Fermentation of bacillus subtilis processing, pet basal feed preparation processing, pet basal feed drying and processing, first time
Cooling treatment, the processing of spray grease, spray fermentation liquor treatment, second of cooling treatment, packing processes, are made antibacterial peptide type pet
Feed.In embodiment 1, swelling temperature is 120 degrees Celsius in pet food particles preparation process, swollen in first time cooling treatment
Change feed granules cooling down to 50 degrees Celsius, vegetable oil is first heated to 50 degrees Celsius again by 1 kilogram in grease spray process
The ratio that 0.09 kilogram of vegetable oil is added in basal feed is sprayed, by 1 kilogram of basal feed in fermentation liquid spray process
The ratio of 0.4 liter of fermentation liquid is added to be sprayed.The water content of antibacterial peptide type feed for pet is made lower than 14%.The withered grass bud used
Spore bacillus number is BNCC179313, is purchased from BeNa Culture Collection Institute of Biotechnology.
Embodiment 2
Fig. 1, antibacterial peptide type feed for pet preparation method of the present invention are please referred to, process flow includes:
Fermentation of bacillus subtilis processing, pet basal feed preparation processing, pet basal feed drying and processing, first time
Cooling treatment, the processing of spray grease, spray fermentation liquor treatment, second of cooling treatment, packing processes, are made antibacterial peptide type pet
Feed.In example 2, swelling temperature is 135 degrees Celsius in pet food particles preparation process, swollen in first time cooling treatment
Change feed granules cooling down to 55 degrees Celsius, vegetable oil is first heated to 55 degrees Celsius again by 1 kilogram in grease spray process
The ratio that 0.1 kilogram of vegetable oil is added in basal feed is sprayed, and is pressed in 1 kilogram of basal feed and is added in fermentation liquid spray process
The ratio of 0.5 liter of fermentation liquid is sprayed.The water content of antibacterial peptide type feed for pet is made lower than 14%.
Embodiment 3
Fig. 1, antibacterial peptide type feed for pet preparation method of the present invention are please referred to, process flow includes:
Fermentation of bacillus subtilis processing, pet basal feed preparation processing, pet basal feed drying and processing, first time
Cooling treatment, the processing of spray grease, spray fermentation liquor treatment, second of cooling treatment, packing processes, are made antibacterial peptide type pet
Feed.In embodiment 3, swelling temperature is 150 degrees Celsius in pet food particles preparation process, swollen in first time cooling treatment
Change feed granules cooling down to 60 degrees Celsius, vegetable oil is first heated to 60 degrees Celsius again by 1 kilogram in grease spray process
The ratio that 0.11 kilogram of vegetable oil is added in basal feed is sprayed, by 1 kilogram of basal feed in fermentation liquid spray process
The ratio of 0.6 liter of fermentation liquid is added to be sprayed.The water content of antibacterial peptide type feed for pet is made lower than 14%.
Embodiment 4
The present embodiment is verifying embodiment, and identifying object is same pet dog (age, weight, the equal class of health status
Like).Control group is fed using pet basal feed, and experimental group is doted on using the antibacterial peptide type prepared in above three embodiments
Object feed is fed, and control variable keeps other feeding conditions all the same.A length of 90 days when Therapy lasted.Specific test result is shown in
Table 1.
The 1 pet dog experimental result table of comparisons of table
Embodiment 5
The present embodiment is verifying embodiment, and identifying object is same pet cat (age, weight, the equal class of health status
Like).Control group is fed using pet basal feed, and experimental group is doted on using the antibacterial peptide type prepared in above three embodiments
Object feed is fed, and control variable keeps other feeding conditions all the same.A length of 90 days when Therapy lasted.Specific test result is shown in
Table 2.
The 2 pet cat experimental result table of comparisons of table
Embodiment 4,5 discussion of results: antibacterial peptide type feed for pet can reach adjust pet intestinal flora, improve immunity,
It makes the life better quality, blast hair color and other effects, therefore its market potential is huge.
Protection scope of the present invention is not limited to the above embodiments, for member of ordinary skill in the art, if
The various changes and deformation carry out to the present invention belong within the scope of the claims in the present invention and equivalent technologies, then meaning of the invention
Including figure is also changed and is deformed comprising these.
Finally it should be noted that: the above examples are only used to illustrate the technical scheme of the present invention rather than its limitations, to the greatest extent
Pipe is described the invention in detail referring to above-described embodiment, it should be understood by those ordinary skilled in the art that: still may be used
With modifications or equivalent substitutions are made to specific embodiments of the invention, and repaired without departing from any of spirit and scope of the invention
Change or equivalent replacement, should all cover in present claims range.
Claims (10)
1. a kind of antibacterial peptide type feed for pet, it is characterised in that: by adding in pet basal feed by bacillus subtilis
The antibacterial peptide fermentation liquid of fermented and cultured is made.
2. a kind of preparation method of antibacterial peptide type feed for pet described in claim 1, it is characterised in that include the following steps:
(1) fermentation process: bacillus subtilis strain is subjected to fermented and cultured, antibacterial peptide fermentation liquid is obtained, is placed in gnotobasis
Under it is spare；
(2) pelletization treatment: preparation pet basal feed, and carry out extruding granulation；
(3) drying and processing: pet basal feed particle is dried；
(4) first time cooling treatment: by the pet basal feed swelling granular cooling down of drying to 50-60 DEG C；
(5) oil spout is handled: by grease even application on pet basal feed swelling granular surface, being placed material after completing operation
It is cooling；
(6) whitewashing is handled: fermentation liquid obtained in step (1) is sprayed to the pet basal feed extruding handled by oil spout
Grain surface；
(7) second of cooling treatment: it pet basal feed swelling granular will be cooled to room temperature after whitewashing is handled, antibacterial is made
Peptide type feed for pet.
3. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (1)
Manufactured antibacterial peptide fermentation liquid potency is not less than 4600 active units/milliliter.
4. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (5)
Grease used is vegetable oil or fish oil.
5. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: step (5) oil
The sprinkling ratio of rouge is to add 0.1 kilogram of grease in 1 kilogram of basal feed.
6. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (5)
Spraying operation is carried out after grease used is heated to 50-60 DEG C again.
7. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (6)
In the ratio sprinkling fermentation liquid for adding 0.5 liter of fermentation liquid in 1 kilogram of basal feed.
8. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (7)
The type of cooling is that aseptic condition leeward is dry and cold but.
9. the preparation method of antibacterial peptide type feed for pet according to claim 7, it is characterised in that: in the step (7)
Being air-dried cooling makes made antibacterial peptide type feed for pet water content lower than 14%.
10. the preparation method of antibacterial peptide type feed for pet according to claim 1, it is characterised in that: in the step (2)
Pet basal feed is subjected to extruding granulation at 120-150 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910650902.2A CN110235988A (en) | 2019-07-18 | 2019-07-18 | A kind of antibacterial peptide type feed for pet and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111772036A (en) * | 2020-07-07 | 2020-10-16 | 陕西科技大学 | Puffed antibacterial peptide pet dog food and preparation method thereof |
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|---|---|---|---|---|
| CN104245943A (en) * | 2012-02-21 | 2014-12-24 | 杜邦营养生物科学有限公司 | Composition comprising fermentation products from bacillus subtilis |
| CN105795113A (en) * | 2016-04-12 | 2016-07-27 | 江苏益品实业有限公司 | Method for efficiently preparing bacillus coagulans pet food and application of bacillus coagulans pet food |
| CN107183352A (en) * | 2017-07-13 | 2017-09-22 | 陕西科技大学 | A kind of preparation technology of antibacterial peptide biological feedstuff |
| KR20180000531A (en) * | 2016-06-23 | 2018-01-03 | 건국대학교 산학협력단 | Antimicrobial peptides with high synergistic effect with antibiotics against multidrug resistant gram-negative bacteria and their uses |
| CN108244352A (en) * | 2018-03-09 | 2018-07-06 | 浙江中大宠物食品有限公司 | A kind of preparation method of yellow wine yeast feed for pet |
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2019
- 2019-07-18 CN CN201910650902.2A patent/CN110235988A/en active Pending
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| CN104245943A (en) * | 2012-02-21 | 2014-12-24 | 杜邦营养生物科学有限公司 | Composition comprising fermentation products from bacillus subtilis |
| CN105795113A (en) * | 2016-04-12 | 2016-07-27 | 江苏益品实业有限公司 | Method for efficiently preparing bacillus coagulans pet food and application of bacillus coagulans pet food |
| KR20180000531A (en) * | 2016-06-23 | 2018-01-03 | 건국대학교 산학협력단 | Antimicrobial peptides with high synergistic effect with antibiotics against multidrug resistant gram-negative bacteria and their uses |
| CN107183352A (en) * | 2017-07-13 | 2017-09-22 | 陕西科技大学 | A kind of preparation technology of antibacterial peptide biological feedstuff |
| CN108244352A (en) * | 2018-03-09 | 2018-07-06 | 浙江中大宠物食品有限公司 | A kind of preparation method of yellow wine yeast feed for pet |
Non-Patent Citations (1)
| Title |
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| 刘伟等: "抗菌肽在养猪生产中的应用", 《养猪》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111772036A (en) * | 2020-07-07 | 2020-10-16 | 陕西科技大学 | Puffed antibacterial peptide pet dog food and preparation method thereof |
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Application publication date: 20190917 |