CN110221422B - Uniform light microscopic lighting device based on Mie scattering - Google Patents
Uniform light microscopic lighting device based on Mie scattering Download PDFInfo
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- CN110221422B CN110221422B CN201910585573.8A CN201910585573A CN110221422B CN 110221422 B CN110221422 B CN 110221422B CN 201910585573 A CN201910585573 A CN 201910585573A CN 110221422 B CN110221422 B CN 110221422B
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- 239000004065 semiconductor Substances 0.000 claims description 2
- 238000005286 illumination Methods 0.000 abstract description 37
- 238000000034 method Methods 0.000 abstract description 3
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- 230000007170 pathology Effects 0.000 description 5
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- 210000003701 histiocyte Anatomy 0.000 description 1
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
Abstract
The invention relates to the technical field of illumination by a miniaturized low-power-consumption light source, in particular to a device and a method for illuminating uniformly based on Mie scattering, which are applied to the field of microscope light sources and solve the problems of complex structure, difficult miniaturization and high product price of the traditional uniform light source. A uniform light microscopic lighting device based on Mie scattering comprises a light source, a Mie scattering device, an optical cavity and a condenser lens. The Mie scattering device is a solid transparent or semitransparent optical device distributed with Mie scattering medium particles, and is arranged in an optical cavity, light emitted by a light source forms uniform irradiation light through the Mie scattering device, and the uniform irradiation light is intensively irradiated on a focus of a condensing lens through the condensing lens. The invention has simple and miniaturized structure, low power consumption, uniform illumination, stable performance, long service life and low cost, and is suitable for the microscope light source, in particular to the field of small optical microscope light sources.
Description
Technical Field
The invention relates to the technical field of illumination by a miniaturized low-power-consumption light source, in particular to a device and a method for illuminating by uniform illumination based on Mie scattering, which are applied to the field of microscope light sources.
Background
The light source system of the optical microscope is used for solving the problems that the irradiated light is generally Gaussian distributed, the brightness of the central area of the microscopic field is highest, the brightness of the periphery is lower, and the illumination is uneven, the problems are reflected in the digital microscope, particularly in the field of digital pathology full-section imaging, the digital images of a plurality of microscopic fields are required to be spliced, the uneven illumination causes bright and dark fluctuation in the spliced digital pathology images, and the brightness fluctuation is required to be compensated through a digital image brightness adjustment algorithm, otherwise, visual fatigue of a diagnostician is caused, and the diagnosis efficiency and the diagnosis precision are reduced. In general, digital pathology images are large in size, and brightness compensation is performed on such large data, so that the calculation efficiency is low, and the whole time consumption of digital pathology imaging is large.
In order to solve the above problems, a high-end microscope system generally adopts a specially designed illumination lens, typically kohler illumination, and the illumination uses a light collecting lens, a field diaphragm, a condenser diaphragm and a condenser lens, which are sequentially arranged between a light source and a sample, so that very uniform sample illumination can be generated, and adverse effects caused by uneven illumination light distribution are ensured to be invisible in an image. However, such an illumination optical structure is complicated, and the use of more lenses brings about an increase in cost, which is disadvantageous in downsizing and cost reduction of the illumination system.
Disclosure of Invention
In order to solve the problem of uneven illumination of the microscopic illumination, realize the microscopic illumination with small size, low cost, low power consumption and long service life, the invention provides a uniform light microscopic illumination device based on Mie scattering.
The technical principle of the invention is as follows: a uniform light microscopic lighting device based on Mie scattering is characterized in that a core device is a solid optical device filled with Mie scattering medium particles, the device is transparent or semitransparent solid, and is made of optical resin or optical glass, and the device has the property of uniformly scattering and outputting incident light beams. And a light source and a condenser lens are respectively arranged at two sides of the Mie scattering device, one end of the light source is a light beam incident end, and one end of the condenser lens is a light beam output end. In order to prevent the incident light from transmitting outwards from the device after entering the Mie scattering device, the hollow lens barrel is wrapped outside the Mie scattering device, so that the illumination utilization rate of the light source is improved, the inner wall of the lens barrel can be subjected to total reflection film coating treatment, and in order to further reduce the cost and ensure that the illumination brightness is enough, the inner wall of the lens barrel can be subjected to black oxidation treatment.
When the light beam emitted by the light source irradiates into the Mie scattering device, a large amount of Mie scattering occurs when the light beam encounters the particles due to the fact that medium particles are filled in the device, the scattered light is continuously scattered and overlapped in the process of passing through the Mie scattering device, and finally uniform light is output.
When the inner wall of the lens barrel is a total reflection coating, light scattered in the Mie scattering device is reflected by the inner wall of the lens barrel and continuously scattered, overlapped and reflected in the period, so that the light intensity loss is less, and the light intensity output by the Mie scattering device is brighter.
When the inner wall of the lens barrel is subjected to black oxidation treatment, light scattered in the Mie scattering period irradiates the inner wall of the lens barrel, and the light is not reflected or is extremely weak, so that the loss of light transmitted in the Mie scattering device is large, and the output light intensity in the Mie scattering period is weak.
When the light source adopts a color light emitting diode, three light emitting diodes of red light, blue light and green light cannot be integrated at the same position due to the diode manufacturing process, and three focuses at different positions are formed through the collecting lens, so that microscopic observation is caused to generate color deviation. However, based on the mie scattering theory, the degree of mie scattering is independent of wavelength, and the properties of photons after scattering remain regardless, so that through the mie scattering device, although three light emitting diodes are at different positions, a stable uniform white output is obtained and a focus can be formed by a condenser lens.
When the light source adopts white light emitting diodes, the spectral components of the white light emitted by each diode are different, but based on the Mie scattering theory, the white light output by the Mie scattering device can form stable and uniform white light output despite the different light emitting diodes, so that the stability of the light source is improved.
Compared with the prior art, the invention has the following advantages:
(1) The white light output by the invention has uniform illumination and spectrum distribution, the illumination light of the traditional microscopic light source is in Gaussian distribution, and the spectrum distribution of different illuminations is not necessarily the same.
(2) The invention can reduce the volume of the light source, ensure higher light source utilization rate, and ensure the utilization rate of the light source because the traditional light source has large volume.
(3) The invention has simple structure and simple assembly, realizes focusing irradiation, only needs one optical lens, and the traditional light source can realize relatively uniform irradiation by combining a plurality of lenses.
(4) The light source adopts the light emitting diode, has low power consumption and low price, and can be continuously used for more than 5 ten thousand hours, so compared with the traditional light source, the light source is stable and reliable and has long service time.
In summary, the invention can realize a compact, low cost, low power consumption, long service life and uniform light microscopic lighting device.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of a uniform light micro-illuminator based on Mie scattering according to the present invention;
FIG. 2 is a schematic view of the light path of a uniform light micro-illuminator based on Mie scattering according to the present invention;
FIG. 3 is a schematic illustration of an application of a Mie scattering-based uniform light micro-illuminator in digital pathology micro-imaging;
FIG. 4 is a microscopic image of illumination using a uniform light microscopic illumination device based on Mie scattering in accordance with the present invention;
fig. 5 is a microscopic image when illuminated using a conventional microscopic illumination device.
Reference numerals: 101-a light source; 102-an optical cavity; 103-mie scattering devices; 104-mie scattering medium particles; 105-condensing lens; 201-light emitted by a light source; 202-light scattered by particles of a Mie scattering medium; 203-scattered light striking the optical cavity wall for reflection; 204-light emitted by a mie scattering device; 205-light collected by a collection mirror; 206-converging the light to form an illumination surface; 301-a micro LED light emitting chip; 302-UV curing the optical adhesive; 303-Mie scattering micro rods; 304-nanospheres; 305-transparent polycarbonate; 306-a lens barrel; 307-plano-convex lenses; 308-emitting light rays; 309-slide; 310-observed cells; 311-cover slips; 312-microobjective; 402-the center of the image is bright with conventional illumination; 403-dark around the image when using conventional illumination.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and rear … …) are included in the embodiments of the present invention, the directional indications are merely used to explain the relative positional relationship, movement conditions, etc. between the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are correspondingly changed.
A uniform light micro-lighting device based on mie scattering according to the present invention will be described in further detail below, but the scope of the present invention should not be limited thereto.
The invention aims to provide a uniform light microscopic lighting device based on Mie scattering, which provides a solution for microscopic observation or image acquisition with uniform image background brightness, in particular to digital pathological full-section imaging.
As shown in fig. 1, a uniform light microscopic illumination device based on mie scattering includes a light source 101, a mie scattering device 103, an optical cavity 102, and a condenser lens 105. Wherein the light source 101 may be a miniaturized low power consumption light source. The light source 101 is located on one side of the Mie scattering device 103 and the collection mirror 105 is located on the other side of the Mie scattering device 103. The mie scattering device 103 is sleeved in the optical cavity 102. The light source 101 is configured to emit light onto the mie scattering device 103. The mie scattering device 103 is used for diverging and radiating the light emitted by the light source 101 onto the condenser lens 105; the condenser lens 105 is used for focusing and outputting light incident from the mie scattering device 103.
Fig. 2 is a schematic light path diagram of a uniform light micro-illuminator based on mie scattering according to the present invention. Wherein arrow 201 is light emitted by the light source 101, arrow 202 is light scattered by the mie scattering medium particles 104, arrow 203 is light reflected by the scattered light impinging on the optical cavity wall, arrow 204 is light emitted by the mie scattering device 103, arrow 205 is light collected by the collecting mirror 105, and 206 is an illumination plane formed by the collected light.
The light source 101 may be a light emitting diode or a semiconductor laser. The mie scattering device 103 may be a solid optical device with particles 104 of a mie scattering medium distributed. The mie scattering medium particles 104 are distributed inside the solid optic. The optical device is transparent or translucent. The refractive index of the optics is less than the refractive index of the mie scattering medium particles 104. The optical cavity 102 may be a hollow cylindrical closed cavity with two ends open, which is wrapped outside the mie scattering device 103, and its inner wall is a mirror surface that reflects light, or a black oxide layer surface. The condenser lens 105 is an optical lens or an optical lens combination capable of converging and irradiating uniform light in a direction of propagation.
In a specific application example, as shown in fig. 3, the light source 101 may be a micro LED light emitting chip 301, the mie scattering device 103 is a mie scattering micro rod 303, the optical cavity 102 is a lens barrel 306, and the condenser 105 is a plano-convex lens 307. In this example, the uniform light micro-lighting device based on Mie scattering includes a micro LED light emitting chip 301, mie scattering micro rods 303, a lens barrel 306, and a plano-convex lens 307. The Mie scattering nanorod 303 is a base material of transparent polycarbonate 305, wherein a doped lactic-co-glycolic acid Polymer (PLGA) or polyvinyl alcohol (PVA) is used as a material, and the diameter of the small cylindrical optical device is 1-100 nm of nano-microsphere 304, and the nano-microsphere 304 is the Mie scattering medium particle 104. The diameter of the column body is the same as or similar to that of the LED light-emitting part, the refractive index of the polycarbonate is smaller than that of the nano-microspheres, and the nano-microspheres are distributed randomly, so that incident light can be irradiated to the nano-microspheres, and Mie scattering can be generated. The lens cone 306 is an opaque hollow cylinder wrapping the surface of the Mie scattering micro rod 303, and the inner wall of the lens cone is provided with a light reflection mirror surface coating film, so that Mie scattering light can be reflected, and the emergent light is ensured to have stronger brightness. The plano-convex lens 307 is a lens having two surfaces, one surface is a plane and the other surface is a sphere, so that the light emitted from the Mie scattering micro rod 303 is converged, and the distance between the converging points is equal to the focal distance of the plano-convex lens 307. The micro LED light emitting chip 301 and the plano-convex lens 307 are adhered to the input end and the output end of the mie scattering micro rod respectively by using UV curing optical adhesive 302, the light emitting surface of the micro LED light emitting chip 301 is adhered to the surface of the input end of the mie scattering micro rod 303, and the plane surface of the plano-convex lens 307 is adhered to the output end of the mie scattering micro rod 303.
An embodiment of the uniform light micro-lighting device based on Mie scattering is as follows: as shown in fig. 3, the micro LED light emitting chip 301 is first energized, the LED surface emits light, and the light beam is scattered by mie scattering in the mie scattering micro rod 303, so that uniform white light is output from the mie scattering micro rod 303 and irradiates on the planar surface of the plano-convex lens 307, and the uniform white light, i.e., the outgoing light 308 of the plano-convex lens 307 is focused and irradiated on the focal point of the plano-convex lens 307 by refraction of the plano-convex lens 307. At this time, if the observed object is the observed cell 310 on the tissue section, the tissue section is located between the slide 309 and the cover slip 311. The tissue slice is positioned at the uniform white light converging focus, the uniform white light is transmitted through the tissue slice to form object light, the object light is irradiated into a microscope objective 312 of the optical microscope, the object light is amplified and imaged on a digital image sensor through a microscope optical system, and a digital microscopic image is formed and displayed on a display through reading and encoding of digital image sensor data by a computer. Since the light irradiated on the histiocyte is white light which is uniformly irradiated, on the digital microscopic image, a background with different brightness in gaussian distribution of the light emitted by the original LED cannot be observed, but an image with uniform background brightness is obtained.
Wherein, fig. 4 is a microscopic image when illuminated using a uniform light microscopic illumination device based on mie scattering according to the present invention, and fig. 5 is a microscopic image when illuminated using a conventional microscopic illumination device. 402 in fig. 5 is the bright center of the image when conventional illumination is used, and 403 is the dark surrounding of the image when conventional illumination is used. By comparing the fig. 4 and 5, it can be seen that the white light outputted by the present invention has uniform illumination and uniform spectrum distribution; the illumination light of the traditional microscopic light source is in Gaussian distribution, and the illumination light is unevenly distributed.
What needs to be explained here is: under the condition of no conflict, the technical features related to the examples can be combined with each other according to actual situations by a person skilled in the art so as to achieve corresponding technical effects, and specific details of the combination situations are not described in detail herein.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above examples, and all technical solutions belonging to the concept of the present invention belong to the protection scope of the present invention. It should be noted that modifications and adaptations to the present invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be within the scope of the present invention.
Claims (1)
1. A uniform light microscopic lighting device based on Mie scattering is characterized by comprising a light source, a Mie scattering device, an optical cavity and a condenser lens;
the light source is positioned on one side of the Mie scattering device, the collecting lens is positioned on the other side of the Mie scattering device, and the Mie scattering device is sleeved in the optical cavity; the light source is used for emitting light to irradiate the Mie scattering device; the Mie scattering device is used for radiating light emitted by the light source to the condenser lens in a divergent manner; the condensing lens is used for focusing and outputting the light emitted by the Mie scattering device;
the light source is a light emitting diode or a semiconductor laser; the Mie scattering device is a solid optical device distributed with Mie scattering medium particles, the optical device is transparent or semitransparent, and the refractive index of the optical device is smaller than that of the Mie scattering medium particles; the optical cavity is a hollow cylindrical closed cavity which is wrapped outside the Mie scattering device and is provided with openings at two ends, and the inner wall of the optical cavity is a mirror surface for reflecting light or the surface of a black oxide layer; the condenser lens is an optical lens or an optical lens combination capable of converging and irradiating uniform light in a direction of propagation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910585573.8A CN110221422B (en) | 2019-07-01 | 2019-07-01 | Uniform light microscopic lighting device based on Mie scattering |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910585573.8A CN110221422B (en) | 2019-07-01 | 2019-07-01 | Uniform light microscopic lighting device based on Mie scattering |
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| Publication Number | Publication Date |
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| CN110221422A CN110221422A (en) | 2019-09-10 |
| CN110221422B true CN110221422B (en) | 2024-04-02 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6934022B1 (en) * | 1998-12-17 | 2005-08-23 | Leica Microsystems Heidelberg Gmbh | Method for differentiated investigation of diverse structures in preferably biological preparations |
| KR20060091860A (en) * | 2005-02-16 | 2006-08-22 | 한국과학기술원 | Real-time confocal microscopy using dispersion optics |
| CN108594414A (en) * | 2018-07-28 | 2018-09-28 | 深圳锋视科技有限公司 | One kind being used for microscopical remote light source system |
| CN209928126U (en) * | 2019-07-01 | 2020-01-10 | 达科为（深圳）医疗设备有限公司 | Uniform light microscopic lighting device based on Mie scattering |
-
2019
- 2019-07-01 CN CN201910585573.8A patent/CN110221422B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6934022B1 (en) * | 1998-12-17 | 2005-08-23 | Leica Microsystems Heidelberg Gmbh | Method for differentiated investigation of diverse structures in preferably biological preparations |
| KR20060091860A (en) * | 2005-02-16 | 2006-08-22 | 한국과학기술원 | Real-time confocal microscopy using dispersion optics |
| CN108594414A (en) * | 2018-07-28 | 2018-09-28 | 深圳锋视科技有限公司 | One kind being used for microscopical remote light source system |
| CN209928126U (en) * | 2019-07-01 | 2020-01-10 | 达科为（深圳）医疗设备有限公司 | Uniform light microscopic lighting device based on Mie scattering |
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| CN110221422A (en) | 2019-09-10 |
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