CN110201231A - The preparation method and equipment of AI microns of artistic rouge carving fillers - Google Patents

The preparation method and equipment of AI microns of artistic rouge carving fillers Download PDF

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Publication number
CN110201231A
CN110201231A CN201910442214.7A CN201910442214A CN110201231A CN 110201231 A CN110201231 A CN 110201231A CN 201910442214 A CN201910442214 A CN 201910442214A CN 110201231 A CN110201231 A CN 110201231A
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fat
cell
preparation
centrifugal
revolving speed
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丁云凤
王秀杰
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Centrifugal Separators (AREA)

Abstract

The embodiment of the present invention discloses the preparation method and equipment of a kind of AI microns of artistic rouge carving filler, the present invention passes through the cell slept in biological microwave resonance technique activation fat first, then by being once centrifuged coarse sizing, secondary centrifuging fine screening and AI intelligence fine screen choosing, obtain the high fat cell of activity, the revolving speed moment that wherein once centrifugation coarse sizing is turned with 5000-8000 per minute is by 50 times of boost in pressure of fat receiving, wherein the unstable fat cell of institutional framework, which is all squeezed, is broken into liquid oil and is completely separated, and leaves behind the strong micron order fat cell of activity;Secondary centrifuging fine screening and micron order rouge with tens of thousands of turns per minute of revolving speed that activity is strong, it is finally equipped with P cell-stimulating again, so that the fat cell activity for filling is high and maintains activity for a long time, the filled survival rate of fat is improved, thus the problems such as being easy to appear elderly person for whom a birthday celebration is being held's volume, puffiness of face, face scleroma after solving filling.

Description

The preparation method and equipment of AI microns of artistic rouge carving fillers
Technical field
The present invention relates to medical and beauty treatment technical field more particularly to a kind of preparation methods of AI microns of artistic rouge carving filler And equipment.
Background technique
It collapses to improve the bridge of the nose, geisoma collapses, chin is short, apple flesh is not full, temple recess, zygomatic arch height, forehead insatiable hunger Expire, have sunken cheeks, lip is thin, decree line, tear ditch, cutis laxa, old problem at the beginning of the face skin such as sagging, generally use from body fat Fat filling technique, but due to fat-extraction position is not scientific and fat in containing a large amount of activity low fat the problems such as, it is whole Species activity is low, is not easy to merge with body after implantation and survive, and finally makes implantation corresponding site destroy body contour, fills out The problems such as elderly person for whom a birthday celebration is being held's volume, puffiness of face, face scleroma are easy to appear after filling.
Summary of the invention
The present invention provides the preparation method and equipment of a kind of AI microns of artistic rouge carving filler, is resonated skill by biological microwave The cell slept in art activation fat, then the high fat cell of activity is only left and taken by screening, and be equipped with the activation of P cellular energy, So that the fat cell activity for filling is high and maintains activity for a long time, avoid low activity object occur in filler before filling Matter improves fat so that merging after filler of the present invention filling with the abundant generation of other body parts lipid structure biology Filled survival rate, thus the problems such as being easy to appear elderly person for whom a birthday celebration is being held's volume, puffiness of face, face scleroma after solving filling.
First aspect present invention provides a kind of preparation method of AI microns of artistic rouge carving filler, includes the following steps:
(1) microwave activation: the vibration frequency of adjustment biological microwave resonance, with the vibration frequency under starvation environment Biological microwave acts on the autologous fat of extraction, the cell slept in activation fat, and enhances the activity of its cell;
(2) primary centrifugation: under starvation environment, centrifugal treating is carried out to through the processed fat of step (1) activation, together When pressed with gyroscopic compass, centrifugal rotational speed be 5000-8000 per minute turn;
(3) secondary centrifuging: under starvation environment, centrifugal treating is carried out to the fat crossed through step (2) centrifugal treating, together When pressed with gyroscopic compass, centrifugal rotational speed be 50000-80000 per minute turn;
(4) it intelligent screening: under starvation environment, is survived using AI intelligent screening technology to through step (3) centrifugal treating The micron order fat to get off carries out intelligent screening, filters out active micron order fat cell;
(5) P cellular energy activates: under starvation environment, injecting in the micron order fat cell that filters out to step (4) P cell carves filler up to AI microns of rouge in normal-temperature reaction 2.5 hours.
In the present invention, rouge to be processed is increased by the cell slept in biological microwave resonance technique activation fat first The quantity of active fat cell in fat, then by being once centrifuged coarse sizing, secondary centrifuging fine screening and AI intelligence fine screen choosing, The high fat cell of activity is obtained, then is equipped with P cell-stimulating, so that the fat cell activity for filling is high and ties up for a long time Hold activity.The wherein boost in pressure 50 that once centrifugation coarse sizing is born fat with 5000-8000 turns of revolving speed moment per minute Times, wherein the unstable fat cell of institutional framework, which is all squeezed, is broken into liquid oil and is completely separated, and leaves behind the strong micron order of activity Fat cell;The strong micron order fat of activity is pressurizeed with tens of thousands of turns per minute of revolving speed once again again and is mentioned by secondary centrifuging fine screening It is pure;Finally by AI intelligently purification filter out with high active fat cell so that filler of the present invention fill after with body The abundant generation biology fusion of the other position lipid structures of body, improves the filled survival rate of fat, to solve filling After be easy to appear elderly person for whom a birthday celebration is being held's volume, puffiness of face, face scleroma the problems such as.
Preferably, the vibration frequency that biological microwave resonates in the step (1) is 5000-7000 times per minute.
Preferably, biological microwave resonance time is 20min in the step (1).
Preferably, in the step (2), a centrifugal work time is 5min.
Preferably, in the step (2), the revolving speed being once centrifuged is 6000 turns per minute.
Preferably, in the step (3), the secondary centrifuging working time is 3min.
Preferably, in the step (3), the revolving speed of secondary centrifuging is 75000 turns per minute.
Preferably, in the step (5), the quantity ratio of P cell and micron order fat cell is 1 ﹕, 2~1 ﹕ 5.
Preferably, in the step (5), the quantity ratio of P cell and micron order fat cell is 1 ﹕ 3.75.
A kind of Preparation equipment of AI microns of artistic rouge carving filler, including vacuum (-tight) housing and the biology being arranged in vacuum (-tight) housing Microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI intelligent screening device and mixing arrangement, the biology Pass through between microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI intelligent screening device and mixing arrangement Materail tube connection;
The fatty inlet being arranged in outside vacuum (-tight) housing is connected at the top of the biological microwave resonance device;
The solvent injection being arranged in outside vacuum (-tight) housing is connected at the top of the slow-speed of revolution centrifugal device, high revolving speed centrifugal device Mouthful, the bottom of slow-speed of revolution centrifugal device, high revolving speed centrifugal device and AI intelligent screening device, which is connected with, to be arranged in outside vacuum (-tight) housing Waste outlet;
The P cell inlet being arranged in outside vacuum (-tight) housing is connected at the top of the mixing arrangement, the bottom of mixing arrangement is connected with Discharge port outside vacuum (-tight) housing is set;
The fat inlet, solvent inlet, waste outlet, P cell inlet and discharge port are infused with corresponding Emitter sealing cooperation;
It is provided in the vacuum (-tight) housing as biological microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI The Vltrasonic device of intelligent screening device and the offer ultrasonic wave of mixing arrangement two sides.
Compared with prior art, the beneficial effects of the present invention are:
1, biological microwave has activated the cell slept in fat, and enhances the activity of its cell, and it is thin to increase active fat The quantity of born of the same parents;
2, the filler provided, substantially high activity fat cell improve filler and other body parts fat The amalgamation of structure improves the filled survival rate of fat;
3, entire purification process is totally-enclosed, is not exposed in air, and adipose tissue is not in oxidation reaction, both guarantees Cell is fresh, in turn ensures bioactivity.
Detailed description of the invention
The attached drawing constituted part of this application is used to provide further understanding of the present application, so that the application's is other Feature, objects and advantages become more apparent upon.The illustrative examples attached drawing and its explanation of the application is for explaining the application, not Constitute the improper restriction to the application.In the accompanying drawings:
Fig. 1 is that the AI micron rouge provided in an embodiment of the present invention based on the activation of P cellular energy carves filler preparation method Flow chart;
Fig. 2 is equipment schematic diagram provided in an embodiment of the present invention;
Figure label is respectively as follows: 1, vacuum (-tight) housing;2, biological microwave resonance device 3, Vltrasonic device;4, slow-speed of revolution centrifugation dress It sets;5, high revolving speed centrifugal device;6, AI intelligent screening device;7, mixing arrangement;8, discharge port;9, P cell inlet;10, molten Agent inlet;11, waste outlet;12, fatty inlet.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description.
The embodiment of the present invention based on P cellular energy activation AI micron rouge carve filler preparation step as shown in Figure 1, Below in conjunction with attached drawing 1, to the AI micron rouge carving filler and system provided in an embodiment of the present invention based on the activation of P cellular energy Preparation Method describes in detail.
Referring to Figure 1, for the embodiment of the invention provides a kind of AI micron rouge based on the activation of P cellular energy to carve filler Preparation flow schematic diagram.
As shown, implementing equipment of the invention are as follows: including vacuum (-tight) housing 1 and the biological microwave being arranged in vacuum (-tight) housing 1 Resonance device 2, slow-speed of revolution centrifugal device 4, high revolving speed centrifugal device 5, AI intelligent screening device 6 and mixing arrangement 7, the biology Between microwave resonance device 2, slow-speed of revolution centrifugal device 4, high revolving speed centrifugal device 5, AI intelligent screening device 6 and mixing arrangement 7 It is connected to by materail tube;
The top of the biological microwave resonance device 2 is connected with the fatty inlet 12 being arranged in outside vacuum (-tight) housing 1;
The slow-speed of revolution centrifugal device 4, high revolving speed centrifugal device 5 top be connected be arranged in outside vacuum (-tight) housing 1 solvent note Entrance 10, the bottom of slow-speed of revolution centrifugal device 4, high revolving speed centrifugal device 5 and AI intelligent screening device 6, which is connected with, to be arranged in vacuum Waste outlet 11 outside cover 1;
The top of the mixing arrangement 7 is connected with the P cell inlet 9 being arranged in outside vacuum (-tight) housing 1, the bottom of mixing arrangement 7 It is connected with the discharge port 8 being arranged in outside vacuum (-tight) housing 1;
The fat inlet 12, solvent inlet 10, waste outlet 11, P cell inlet 9 and discharge port 8 with Corresponding syringe sealing cooperation.
In order to be sufficiently mixed/dissolve substance, be provided in the vacuum (-tight) housing 1 for biological microwave resonance device 2, the slow-speed of revolution from Center device 4,7 two sides of high revolving speed centrifugal device 5, AI intelligent screening device 6 and mixing arrangement offer ultrasonic wave Vltrasonic device 3。
As shown in Figure 1, the embodiment of the present invention the method includes the steps S01- step S05, it is described in detail below:
Vacuum (-tight) housing 1 vacuumizes;
S01, microwave activation: the autologous fat of extraction is injected into biological microwave resonance device 2 through fatty inlet 12, The vibration frequency for adjusting biological microwave resonance acts on extraction with the biological microwave of the vibration frequency under starvation environment Autologous fat activates the cell slept in fat, and enhances the activity of its cell, and the vibration frequency of biological microwave resonance is every point Clock 6500 times, resonance time 20min, resonance is completed, and fat is transferred to slow-speed of revolution centrifugal device 4 through materail tube;
S02, it is primary to be centrifuged: carry out centrifugal treating to through the processed fat of step S01 activation, at the same with gyroscopic compass into Row pressure, adjusting centrifugal rotational speed is 6000 turns per minute, working time 5min, after centrifugation, by waste material through waste outlet 11 produce, and inject solvent through solvent inlet 10, open Vltrasonic device 3, viscous wall material dissolution is transferred to high revolving speed through materail tube Centrifugal device 5;
Secondary centrifuging: S03 carries out centrifugal treating to the fat crossed through centrifugal treating of step S02, while with gyro power Press, and adjusting centrifugal rotational speed is 75000 turns per minute, working time 3min, after centrifugation, by waste material through waste material Outlet 11 is produced, and is injected solvent through solvent inlet 10, is opened Vltrasonic device 3, viscous wall material dissolution is transferred to AI through materail tube Intelligent screening device 6;
S04, intelligent screening: using AI intelligent screening technology to the micron order rouge survived through step S03 centrifugal treating Fat carries out intelligent screening, filters out active micron order fat cell and is transferred to mixing arrangement 7 through materail tube.
The activation of S05, P cellular energy: it is infused by P cell inlet 9 into the micron order fat cell that step S04 is filtered out Enter P cell, carves filler up to AI microns of rouge within normal-temperature reaction 2~3 hours.
The filler that the present embodiment obtains, preparation process are as follows: first by biological microwave resonance technique activation fat The cell of sleep increases the quantity of active fat cell in fat to be processed, then by being once centrifuged coarse sizing, secondary centrifuging Fine screening and AI intelligence fine screen choosing, obtain the high fat cell of activity, then be equipped with P cell-stimulating, so that for filling Fat cell activity is high and maintains activity for a long time.The wherein revolving speed wink that once centrifugation coarse sizing is turned with 5000-8000 per minute Between bear fat 50 times of boost in pressure, wherein the unstable fat cell of institutional framework is all squeezed that be broken into liquid oil thorough Separation leaves behind the strong micron order fat cell of activity;Secondary centrifuging fine screening again will be active with tens of thousands of turns per minute of revolving speed Strong micron order fat pressurizes purification once again;Finally by AI, intelligently purification is filtered out with high active fat cell, is made It is merged after obtaining filler filling of the present invention with the abundant generation of other body parts lipid structure biology, after improving fat filling Survival rate, thus solve filling after be easy to appear elderly person for whom a birthday celebration is being held's volume, puffiness of face, face scleroma the problems such as.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly It encloses, therefore equivalent changes made in accordance with the claims of the present invention, is still within the scope of the present invention.

Claims (10)

1. the preparation method of a kind of AI microns artistic rouge carving filler, which comprises the steps of:
(1) microwave activation: the vibration frequency of adjustment biological microwave resonance, with the biology of the vibration frequency under starvation environment The cell that microwave action is slept in the autologous fat of extraction, activation fat, and enhance the activity of its cell;
(2) primary centrifugation: under starvation environment, carrying out centrifugal treating to through the processed fat of step (1) activation, while with Gyroscopic compass presses, and centrifugal rotational speed turns for 5000-8000 per minute;
(3) secondary centrifuging: under starvation environment, to the fat progress centrifugal treating crossed through step (2) centrifugal treating, while with Gyroscopic compass presses, and centrifugal rotational speed turns for 50000-80000 per minute;
(4) it intelligent screening: under starvation environment, is survived using AI intelligent screening technology to through step (3) centrifugal treating Micron order fat carry out intelligent screening, filter out active micron order fat cell;
(5) P cellular energy activates: under starvation environment, injection P is thin in the micron order fat cell that filters out to step (4) Born of the same parents carve filler up to AI microns of rouge in normal-temperature reaction 2~3 hours.
2. preparation method according to claim 1, which is characterized in that the vibration that biological microwave resonates in the step (1) Frequency is 5000-7000 times per minute.
3. preparation method according to claim 2, which is characterized in that biological microwave resonance time is in the step (1) 20min。
4. preparation method according to claim 1, which is characterized in that in the step (2), a centrifugal work time is 5min。
5. the preparation method according to claim 4, which is characterized in that in the step (2), the revolving speed being once centrifuged is every 6000 turns of minute.
6. the preparation method according to claim 4, which is characterized in that in the step (3), the secondary centrifuging working time is 3min。
7. preparation method according to claim 6, which is characterized in that in the step (3), the revolving speed of secondary centrifuging is every 75000 turns of minute.
8. preparation method according to claim 1, which is characterized in that in the step (5), P cell and micron order fat The quantity ratio of cell is 1 ﹕, 2~1 ﹕ 5.
9. preparation method according to claim 8, which is characterized in that in the step (5), P cell and micron order fat The quantity ratio of cell is 1 ﹕ 3.75.
10. the Preparation equipment of a kind of AI microns artistic rouge carving filler, which is characterized in that including vacuum (-tight) housing and be arranged in vacuum Biological microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI intelligent screening device and mixing dress in cover It sets, the biological microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI intelligent screening device and mixing dress It is connected between setting by materail tube;
The fatty inlet being arranged in outside vacuum (-tight) housing is connected at the top of the biological microwave resonance device;
The solvent inlet being arranged in outside vacuum (-tight) housing is connected at the top of the slow-speed of revolution centrifugal device, high revolving speed centrifugal device, it is low The bottom of revolving speed centrifugal device, high revolving speed centrifugal device and AI intelligent screening device is connected with the waste material being arranged in outside vacuum (-tight) housing and goes out Mouthful;
The P cell inlet being arranged in outside vacuum (-tight) housing is connected at the top of the mixing arrangement, the bottom of mixing arrangement is connected with setting Discharge port outside vacuum (-tight) housing;
The fat inlet, solvent inlet, waste outlet, P cell inlet and discharge port with corresponding syringe Sealing cooperation;
It is provided in the vacuum (-tight) housing as biological microwave resonance device, slow-speed of revolution centrifugal device, high revolving speed centrifugal device, AI intelligence The Vltrasonic device of screening plant and the offer ultrasonic wave of mixing arrangement two sides.
CN201910442214.7A 2019-05-24 2019-05-24 The preparation method and equipment of AI microns of artistic rouge carving fillers Pending CN110201231A (en)

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CN201910442214.7A CN110201231A (en) 2019-05-24 2019-05-24 The preparation method and equipment of AI microns of artistic rouge carving fillers

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CN1871343A (en) * 2003-11-04 2006-11-29 株式会社宝玛斯特 Method and system for preparing stem cells from fat tissue
CN107823709A (en) * 2017-08-27 2018-03-23 广州赛度检测服务有限公司 A kind of preparation method of Grainy fat tissue
US20190185814A1 (en) * 2017-12-14 2019-06-20 Lifecell Corporation Device and methods for tissue processing
CN109182081A (en) * 2018-08-22 2019-01-11 长春长光辰英生物科学仪器有限公司 A kind of unicellular separation system based on image processing model

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Application publication date: 20190906