CN110199778A - A kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium - Google Patents
A kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium Download PDFInfo
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- CN110199778A CN110199778A CN201910482144.8A CN201910482144A CN110199778A CN 110199778 A CN110199778 A CN 110199778A CN 201910482144 A CN201910482144 A CN 201910482144A CN 110199778 A CN110199778 A CN 110199778A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a kind of production technologies using fermentation of corn starch production tricholoma matsutake mycelium, belong to tricholoma matsutake mycelium production field, a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium, comprising the following steps: one, slant strains culture；Two, liquid seeds culture；Three, fermented and cultured；Four, dry mycelial weight.This produces the production technology of tricholoma matsutake mycelium using fermentation of corn starch; the experiment proved that the mycelium dry matter fermentation highest yield produced using the technique is in 24.82g/L or more; and mycelium dry matter yield is only in 18g/L or so in the prior art; therefore there is marked improvement compared with the production technology of the mycelium dry matter of the prior art; it can be seen that the fermented and cultured of the suitable Tricholoma of the present invention; mycelia dry matter yield is stable and yield is high, is produced on a large scale, is worthy to be popularized.
Description
Technical field
The present invention relates to tricholoma matsutake mycelium production fields, produce pine using fermentation of corn starch more specifically to a kind of
Fine and soft mycelial production technology.
Background technique
Tricholoma also known as Trichotoma matsutake are a kind of fungies of preciousness.Delicious flavour, it is full of nutrition, there is very high medicinal food
Treatment effect.Tricholoma autumn is born in pine forest or theropencedrymion on the ground, is all distributed in the Northeast, China and southwest, state
Outer Japan and Korea are also distributed, and matsutake cannot be cultivateeed, and are a kind of pure natural rare rare edible mushrooms.
Since the nutritive value and medical value of tricholoma matsutake mycelium are high, annual output successively decreases year by year, it is therefore desirable to reinforcement pair
The tricholoma matsutake mycelium that the breeding of the research of matsutake nursing and breeding skill, especially strain is obtained by liquid fermentation smell and at
It is point upper very close with wild matsutake, and fermented and cultured has many advantages, such as with short production cycle, and condition is easy to control, with liquid fermentation
Mode produces tricholoma matsutake mycelium, and by its extract applied to having very big development prospect in food, cosmetic industry, simultaneously
The protection to matsutake is also helped, tricholoma matsutake mycelium dry matter yield improves unit volume only in 18g/L or so in the prior art
The yield of culture medium mycelium dry matter, still there is larger space.
Summary of the invention
1. technical problems to be solved
Aiming at the problems existing in the prior art, pine is produced using fermentation of corn starch the purpose of the present invention is to provide a kind of
Fine and soft mycelial production technology, has the high advantage of tricholoma matsutake mycelium dry matter yield, solves matsutake mycelia in the prior art
Soma substance yield only in 18g/L or so, improves the yield of unit volume culture medium mycelium dry matter, still there is larger space
Problem.
2. technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium, comprising the following steps:
One, slant strains culture: taking mycoderm not rupture Tricholoma fructification, is that 70% ethyl alcohol is repeated with mass fraction
After surface sterilizing, a fritter is removed with aseptic nipper and knife, access is sealed in slant medium, it is put into incubator,
It is cultivated under 25 DEG C of constant temperatures, after mycelia covers with inclined-plane, is transferred to and cultivates in next step；
Two, slant strains cultured in step 1 liquid seeds culture: are accessed into the liquid seeds culture of shaking flask corn flour
In base, in 25 DEG C, 190r/min shaking table culture 8 days, be sterile filtered to obtain mycelium, clean with aseptic water washing, adds four times of volumes
Physiological saline and the dilution of a small amount of bead smash mycelium pellet, shake up, and obtain liquid seeds.
Three, fermented and cultured: fermentor high pressure sterilization under sterile conditions trains the corn flour liquid fermentation after sterilizing
Feeding base is added in fermentor, and then the cultured liquid seeds of step 2 are transferred in fermentor, are passed through by 0.45 μm
Sterilised membrane filter filtered air cultivated, carry out fermented and cultured one week.
Four, dry mycelial weight: 40 mesh filter screens of culture solution are filtered, isolated mycelium and fermentation liquid, mycelium is through going
After ionized water rinses for several times, vacuum filtration, in 60 DEG C of baking ovens, drying to constant weight, is weighed with assay balance, obtains tricholoma matsutake mycelium
Dry matter.
Preferably, the slant medium includes: corn flour 40g/L, glucose 10g/L, agar 20g/L and excess water,
PH is natural.
Preferably, the shaking flask corn flour liquid seed culture medium includes: corn flour 20g/L, glucose 5g/L, phosphoric acid
Potassium dihydrogen 2g/L, magnesium sulfate 0.5g/L, vitamin B 0.15g/L and excess water.
Preferably, the corn flour liquid fermentation medium include: corn flour 40g/L, wheat bran 15g/L, sucrose 10g/L,
Glucose 10g/L, peptone 5g/L, potassium dihydrogen phosphate 2.5g/L, raw B0.05~0.1g/L, magnesium sulfate 0.5g/L, dimension life are tieed up
Plain B0.15g/L and excess water.
Preferably, the slant medium the preparation method comprises the following steps: corn flour is first dissolved 1h under 60 DEG C of water bath conditions, pass through
Its filtrate is taken after dusting cover filtering, and agar is added into filtrate, heating is boiled again, and is stirred continuously to agar and is completely dissolved,
Then glucose will be added, be stirred continuously to glucose and be completely dissolved, finally plus water constant volume, become the culture medium prepared,
It is distributed into test tube while hot, inclined-plane is put after sterilizing to get slant medium.
Preferably, the shaking flask corn flour liquid seed culture medium the preparation method comprises the following steps: first by corn flour in 60 DEG C of water-baths
Under the conditions of dissolve 1h, its filtrate is taken after dusting cover filters, weighs sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, dimension
Raw element B, is added in filtrate, stirring to dissolution, and adds water constant volume；
Then a rotor is put in shaking flask, is put into shaking flask after culture medium is cooling, by bottleneck and rubber nozzle tampon
It stoppers, and is wrapped up with brown paper, shaking flask is put into pressure cooker sterilizing, shaking flask is put into pressure cooker sterilizing, at 121 DEG C of temperature, pressure
Power is inoculated with after culture medium is cooling in 0.15MPa sterilizing 40min.
Preferably, the corn flour liquid fermentation medium the preparation method comprises the following steps: first by corn flour and wheat bran in 60 DEG C of water
Dissolve 1h under the conditions of bath, its filtrate taken after dusting cover filters, weigh sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate,
Vitamin B is added in filtrate, and dissolved liquid is added in fermentor to dissolving for stirring, and the defoaming agent for adding heat disappears
Bubble opens air pump stirring, adds water to visor middle line constant volume to get corn flour liquid fermentation medium, the defoaming agent is soya-bean oil.
Preferably, the fermentor revolving speed is 150r/min, and ventilation rate 0.8V/Vmin, interior pressure tank stabilization is 0.08
~0.1MPa is cultivated under conditions of 25 DEG C of constant temperature.
Preferably, PHS-3 type acidometer measurement PH is used during the fermented and cultured and carries out pH control fermentation, that is, is risen
Beginning pH is 5.4~5.8, when fermentation liquid pH drops to 4.2~4.5, adds alkaline matter, control fermentation pH maintains 4.5~
4.8 range.
Preferably, the liquid seeds inoculum concentration volume and the volume ratio of fermentor are 1:10, the corn powder liquid hair
The volume ratio of ferment culture medium and fermentor is 7:10.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
(1) production technology that tricholoma matsutake mycelium is produced using fermentation of corn starch: the experiment proved that being produced using the technique
Mycelium dry matter fermentation highest yield out is in 24.82g/L or more, and mycelium dry matter yield only exists in the prior art
18g/L or so, therefore have marked improvement compared with the production technology of the mycelium dry matter of the prior art, it is seen that the present invention is suitble to matsutake
The fermented and cultured of bacterium, mycelia dry matter yield is stable and yield is high, is produced on a large scale, is worthy to be popularized.
(2) it the production technology that tricholoma matsutake mycelium is produced using fermentation of corn starch: is passed through in the fermenter using liquid seeds
The method of corn flour liquid fermentation medium fermented and cultured effectively improves tricholoma matsutake mycelium dry matter yield, and is with corn flour
Major ingredient, glucose are slant medium, shaking flask corn flour liquid seed culture medium and corn flour the liquid fermentation training of auxiliary material preparation
Base simple process and low cost is supported, is worthy to be popularized.
(3) production technology that tricholoma matsutake mycelium is produced using fermentation of corn starch: the experiment proved that using technique we
Compared with the prior art, dry mycelial weight improves 33.05% to case, and fermentation time reduction 1 day, and daily dry mycelial weight is all high
In the dry mycelial weight of the prior art, therefore this programme hypha fermentation speed is fast, with short production cycle, is the scale metaplasia of tricholoma matsutake mycelium
It produces and using reliable basis is provided, is worthy to be popularized.
Detailed description of the invention
Fig. 1 is flow chart of the invention；
Fig. 2 is one result data figure of experimental example of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description；Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the instruction such as term " on ", "lower", "inner", "outside" " top/bottom end "
Orientation or positional relationship be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description the present invention and simplification retouch
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore it is not considered as limiting the invention.In addition, term " first ", " second " are used for description purposes only, and cannot understand
For indication or suggestion relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Be equipped with ", " be arranged/connect ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to removable
Connection is unloaded, or is integrally connected；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements.It for the ordinary skill in the art, can be specific
Situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment one:
Referring to Fig. 1, a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium, comprising the following steps:
One, slant strains culture: taking mycoderm not rupture Tricholoma fructification, is that 70% ethyl alcohol is repeated with mass fraction
After surface sterilizing, a fritter is removed with aseptic nipper and knife, access is sealed in slant medium, it is put into incubator,
It is cultivated under 25 DEG C of constant temperatures, after mycelia covers with inclined-plane, is transferred to and cultivates in next step；
Two, slant strains cultured in step 1 liquid seeds culture: are accessed into the liquid seeds culture of shaking flask corn flour
In base, in 25 DEG C, 190r/min shaking table culture 8 days, be sterile filtered to obtain mycelium, clean with aseptic water washing, adds four times of volumes
Physiological saline and the dilution of a small amount of bead smash mycelium pellet, shake up, and obtain liquid seeds.
Three, fermented and cultured: fermentor high pressure sterilization under sterile conditions trains the corn flour liquid fermentation after sterilizing
Feeding base is added in fermentor, and then the cultured liquid seeds of step 2 are transferred in fermentor, are passed through by 0.45 μm
Sterilised membrane filter filtered air cultivated, carry out fermented and cultured one week.
Four, dry mycelial weight: 40 mesh filter screens of culture solution are filtered, isolated mycelium and fermentation liquid, mycelium is through going
After ionized water rinses for several times, vacuum filtration, in 60 DEG C of baking ovens, drying to constant weight, is weighed with assay balance, obtains tricholoma matsutake mycelium
Dry matter.
Further, slant medium includes: corn flour 40g/L, glucose 10g/L, agar 20g/L and excess water, PH
It is natural.
Slant medium the preparation method comprises the following steps: corn flour is first dissolved 1h under 60 DEG C of water bath conditions, after dusting cover filters
Its filtrate is taken, and agar is added into filtrate, heating is boiled again, and is stirred continuously to agar and is completely dissolved, and will be then added
Glucose is stirred continuously to glucose and is completely dissolved, and finally plus water constant volume, becomes the culture medium prepared, it is divided while hot
It is packed into test tube, inclined-plane is put after sterilizing to get slant medium.
Further, shaking flask corn flour liquid seed culture medium includes: corn flour 20g/L, glucose 5g/L, biphosphate
Potassium 2g/L, magnesium sulfate 0.5g/L, vitamin B 0.15g/L and excess water.
Shaking flask corn flour liquid seed culture medium the preparation method comprises the following steps: first corn flour is dissolved under 60 DEG C of water bath conditions
1h takes its filtrate after dusting cover filters, and weighs sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, is added
Into filtrate, stirring to dissolution, and add water constant volume；
Then a rotor is put in shaking flask, is put into shaking flask after culture medium is cooling, by bottleneck and rubber nozzle tampon
It stoppers, and is wrapped up with brown paper, shaking flask is put into pressure cooker sterilizing, shaking flask is put into pressure cooker sterilizing, at 121 DEG C of temperature, pressure
Power is inoculated with after culture medium is cooling in 0.15MPa sterilizing 40min.
Further, corn flour liquid fermentation medium includes: corn flour 40g/L, wheat bran 15g/L, sucrose 10g/L, Portugal
Grape sugar 10g/L, peptone 5g/L, potassium dihydrogen phosphate 2.5g/L, raw B0.05~0.1g/L, magnesium sulfate 0.5g/L, vitamin are tieed up
B0.15g/L and excess water.
Corn flour liquid fermentation medium the preparation method comprises the following steps: first corn flour and wheat bran are dissolved under 60 DEG C of water bath conditions
1h takes its filtrate after dusting cover filters, and weighs sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, is added
Into filtrate, dissolved liquid is added in fermentor to dissolving for stirring, is added the defoaming agent defoaming of heat, is opened air pump
Stirring, adds water to visor middle line constant volume to get corn flour liquid fermentation medium, defoaming agent is soya-bean oil.
Further, fermentor revolving speed is 150r/min, ventilation rate 0.8V/Vmin, and interior pressure tank, which is stablized, to exist
0.02MPa is cultivated under conditions of 25 DEG C of constant temperature.
Further, PHS-3 type acidometer measurement PH is used during fermented and cultured and carries out pH control fermentation, that is, is originated
PH is 5.4~5.8, when fermentation liquid pH drops to 4.2~4.5, adds alkaline matter, control fermentation pH maintains 4.5~4.8
Range.
Further, liquid seeds inoculum concentration volume and the volume ratio of fermentor are 1:10, corn flour liquid fermentation and culture
The volume ratio of base and fermentor is 7:10.
Experimental example one: it is taken by the hypha fermentation method in this programme embodiment one with hypha fermentation method in the prior art
One week matsutake mycelia carries out dry mycelial weight Experimental comparison, obtains data in Fig. 2；
Tend to growth trend within four days before mycelia dry matter after fermenting 1 day in this programme as shown in Figure 2 in this programme, we
In case dry mycelial weight on day 4 when reach peak, dry mycelial weight at this time is 24.88g/L, after fermentation the 6th day, dry mycelial weight
Start to be decreased obviously, it can thus be appreciated that when Tricholoma fermentation was by second day, starting several phases increases, and indices increase rapidly, new old
Metabolism is very active, and after reaching maximum value, the growth and metabolism of Tricholoma are relative constant, and effective mycelia body weight accumulation reaches
Best Times, after fermenting the 6th day, nutriment is depleted, and mycelia enters the decline phase；
And dry mycelial weight just reached peak at the 5th day in the prior art, and peak is only 18.7g/L, and the 6th day
It begins to be remarkably decreased, while daily dry mycelial weight is below the dry mycelial weight in this programme；
In conclusion this programme is compared with the prior art, dry mycelial weight improves 33.05%, fermentation time reduction 1
It, it can thus be appreciated that this programme hypha fermentation speed is fast, the period is short, provides reliably for the large-scale production and utilization of tricholoma matsutake mycelium
Foundation is worthy to be popularized.
Experimental example two takes the identical about 2cm of size25 pieces of fungus block, respectively number be A, B, C, D, E, by the side of embodiment one
Method is cultivated respectively, and the 4th day mycelia is taken to carry out dry matter check weighing, the result is as follows:
| Experimental example | A | B | C | D | E |
| Mycelium dry matter yield (g/L) | 25.03 | 24.82 | 24.95 | 24.91 | 24.97 |
As seen from the above table, mycelium dry matter fermentation highest yield is produced in 24.82g/L or more by the technique, and
Mycelium dry matter yield is only in 18g/L or so in the prior art, therefore the production work of the mycelium dry matter compared with the prior art
Skill has marked improvement, it is seen that the present invention is suitble to the fermented and cultured of Tricholoma, and mycelia dry matter yield is stable and yield is high, can scale
Metaplasia produces.
The foregoing is intended to be a preferred embodiment of the present invention；But scope of protection of the present invention is not limited thereto.
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
It improves design and is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium, it is characterised in that: the following steps are included:
One, slant strains culture: taking mycoderm not rupture Tricholoma fructification, is that surface is repeated in 70% ethyl alcohol with mass fraction
After sterilizing, a fritter is removed with aseptic nipper and knife, access is sealed in slant medium, incubator is put into, at 25 DEG C
It is cultivated under constant temperature, after mycelia covers with inclined-plane, is transferred to and cultivates in next step；
Two, liquid seeds culture: slant strains cultured in step 1 are accessed in shaking flask corn flour liquid seed culture medium,
In 25 DEG C, 190r/min shaking table culture 8 days, be sterile filtered to obtain mycelium, clean with aseptic water washing, adds four times of volume physiology salts
Water and the dilution of a small amount of bead smash mycelium pellet, shake up, and obtain liquid seeds.
Three, fermented and cultured: by fermentor high pressure sterilization, under sterile conditions by the corn flour liquid fermentation medium after sterilizing
It is added in fermentor, then the cultured liquid seeds of step 2 is transferred in fermentor, are passed through the nothing by 0.45 μm
The air of bacterium membrane filtration is cultivated, and is carried out fermented and cultured one week.
Four, dry mycelial weight: 40 mesh filter screens of culture solution are filtered, isolated mycelium and fermentation liquid, mycelium is through deionization
After water rinses for several times, vacuum filtration, in 60 DEG C of baking ovens, drying to constant weight, is weighed with assay balance, obtains tricholoma matsutake mycelium dry
Matter.
2. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
In: the slant medium includes: corn flour 40g/L, glucose 10g/L, agar 20g/L and excess water, and PH is natural.
3. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
In: the shaking flask corn flour liquid seed culture medium includes: corn flour 20g/L, glucose 5g/L, potassium dihydrogen phosphate 2g/L, sulphur
Sour magnesium 0.5g/L, vitamin B 0.15g/L and excess water.
4. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
In: the corn flour liquid fermentation medium include: corn flour 40g/L, wheat bran 15g/L, sucrose 10g/L, glucose 10g/L,
Peptone 5g/L, potassium dihydrogen phosphate 2.5g/L, raw B0.05~0.1g/L, magnesium sulfate 0.5g/L, vitamin B 0.15g/L and remaining are tieed up
Measure water.
5. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 2, feature exist
In: the slant medium the preparation method comprises the following steps: corn flour is first dissolved 1h under 60 DEG C of water bath conditions, taken after dusting cover filters
Its filtrate, and agar is added into filtrate, heating is boiled again, and is stirred continuously to agar and is completely dissolved, and Portugal will then be added
Grape sugar, is stirred continuously to glucose and is completely dissolved, and finally plus water constant volume, becomes the culture medium prepared, it is dispensed while hot
Enter in test tube, inclined-plane is put after sterilizing to get slant medium.
6. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 3, feature exist
In: the shaking flask corn flour liquid seed culture medium the preparation method comprises the following steps: corn flour is first dissolved 1h under 60 DEG C of water bath conditions,
Its filtrate is taken after dusting cover filters, and is weighed sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, is added to
In filtrate, stirring to dissolution, and add water constant volume；
Then a rotor is put in shaking flask, is put into shaking flask after culture medium is cooling, bottleneck and rubber nozzle are stoppered with tampon,
And wrapped up with brown paper, shaking flask is put into pressure cooker sterilizing, shaking flask is put into pressure cooker sterilizing, at 121 DEG C of temperature, pressure exists
0.15MPa sterilizing 40min, is inoculated with after culture medium is cooling.
7. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 4, feature exist
In: the corn flour liquid fermentation medium the preparation method comprises the following steps: first dissolving corn flour and wheat bran under 60 DEG C of water bath conditions
1h takes its filtrate after dusting cover filters, and weighs sucrose, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, is added
Into filtrate, dissolved liquid is added in fermentor to dissolving for stirring, is added the defoaming agent defoaming of heat, is opened air pump
Stirring adds water to visor middle line constant volume to get corn flour liquid fermentation medium, and the defoaming agent is soya-bean oil.
8. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
In: the fermentor revolving speed is 150r/min, and ventilation rate 0.8V/Vmin, interior pressure tank stabilization is in 0.08~0.1MPa, constant temperature
It is cultivated under conditions of 25 DEG C.
9. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
It using PHS-3 type acidometer measurement PH during: the fermented and cultured and carries out pH control fermentation, i.e. starting pH is 5.4~
5.8, when fermentation liquid pH drops to 4.2~4.5, alkaline matter is added, control fermentation pH maintains 4.5~4.8 ranges.
10. a kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium according to claim 1, feature exist
In: the liquid seeds inoculum concentration volume and the volume ratio of fermentor be 1:10, the corn flour liquid fermentation medium and hair
The volume ratio of fermentation tank is 7:10.
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| CN114468194A (en) * | 2022-03-09 | 2022-05-13 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake active fungus sea cucumber health drink |
| CN114480060A (en) * | 2022-03-09 | 2022-05-13 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake and sea cucumber wine |
| CN114504069A (en) * | 2022-03-09 | 2022-05-17 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake active fungus health drink |
| CN114667890A (en) * | 2021-12-20 | 2022-06-28 | 延边圣泽方圆生物科技有限公司 | Method for separating and culturing strain and mycelium from Tricholoma matsutake fruiting body, and preparation method of Tricholoma matsutake mycelium powder and concentrated solution |
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| CN114667890A (en) * | 2021-12-20 | 2022-06-28 | 延边圣泽方圆生物科技有限公司 | Method for separating and culturing strain and mycelium from Tricholoma matsutake fruiting body, and preparation method of Tricholoma matsutake mycelium powder and concentrated solution |
| CN114468194A (en) * | 2022-03-09 | 2022-05-13 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake active fungus sea cucumber health drink |
| CN114480060A (en) * | 2022-03-09 | 2022-05-13 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake and sea cucumber wine |
| CN114504069A (en) * | 2022-03-09 | 2022-05-17 | 大连富森智能科技有限公司 | Preparation method of tricholoma matsutake active fungus health drink |
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