CN110193512A - The device and method that two-dimentional AC field enhancement microbiological repairs petroleum-type organic material contaminated soil - Google Patents
The device and method that two-dimentional AC field enhancement microbiological repairs petroleum-type organic material contaminated soil Download PDFInfo
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- CN110193512A CN110193512A CN201910425170.7A CN201910425170A CN110193512A CN 110193512 A CN110193512 A CN 110193512A CN 201910425170 A CN201910425170 A CN 201910425170A CN 110193512 A CN110193512 A CN 110193512A
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- contaminated soil
- soil
- type organic
- petroleum
- organic material
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- 239000002689 soil Substances 0.000 title claims abstract description 112
- 239000011368 organic material Substances 0.000 title claims abstract description 22
- 230000008439 repair process Effects 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 18
- 230000015556 catabolic process Effects 0.000 claims abstract description 51
- 238000006731 degradation reaction Methods 0.000 claims abstract description 51
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 34
- 230000033001 locomotion Effects 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 34
- 239000007921 spray Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 26
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 21
- 229910052698 phosphorus Inorganic materials 0.000 claims description 21
- 239000011574 phosphorus Substances 0.000 claims description 21
- 238000005070 sampling Methods 0.000 claims description 21
- 238000007789 sealing Methods 0.000 claims description 19
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims description 17
- 238000012544 monitoring process Methods 0.000 claims description 16
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 12
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 12
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 239000000654 additive Substances 0.000 claims description 11
- 230000000996 additive effect Effects 0.000 claims description 11
- 229910052799 carbon Inorganic materials 0.000 claims description 11
- 235000010344 sodium nitrate Nutrition 0.000 claims description 9
- 239000004317 sodium nitrate Substances 0.000 claims description 9
- 235000014786 phosphorus Nutrition 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 229930195733 hydrocarbon Natural products 0.000 claims description 4
- 150000002430 hydrocarbons Chemical class 0.000 claims description 4
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229910001868 water Inorganic materials 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 claims description 2
- 230000008602 contraction Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000008121 dextrose Substances 0.000 claims 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 1
- 235000011152 sodium sulphate Nutrition 0.000 claims 1
- 239000003921 oil Substances 0.000 abstract description 34
- 239000000126 substance Substances 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 11
- 238000005067 remediation Methods 0.000 abstract description 9
- 239000003208 petroleum Substances 0.000 abstract description 6
- 238000006056 electrooxidation reaction Methods 0.000 abstract description 5
- 239000003344 environmental pollutant Substances 0.000 abstract description 5
- 231100000719 pollutant Toxicity 0.000 abstract description 5
- 238000012546 transfer Methods 0.000 abstract description 5
- 230000006698 induction Effects 0.000 abstract description 4
- 230000000050 nutritive effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 230000005669 field effect Effects 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 description 9
- 230000005684 electric field Effects 0.000 description 9
- 244000005700 microbiome Species 0.000 description 8
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 229910052739 hydrogen Inorganic materials 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 229910000754 Wrought iron Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- GTTYPHLDORACJW-UHFFFAOYSA-N nitric acid;sodium Chemical compound [Na].O[N+]([O-])=O GTTYPHLDORACJW-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- -1 phosphoric acid hydrogen Chemical class 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
- B09C1/085—Reclamation of contaminated soil chemically electrochemically, e.g. by electrokinetics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Soil Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses the device and methods that a kind of two-dimentional AC field enhancement microbiological repairs petroleum-type organic material contaminated soil, the present invention applies the AC field of two-dimensional quadrature in the bioreactor of petroleum-type organic material contaminated soil reparation, using the electrokinesis and electrochemical oxidation process under AC field effect, strengthen oil degradation microbial inoculum to the degradation effect of petroleum type organic.The electrokinesis of the AC field induction of two-dimensional quadrature can promote the reciprocating motion that substance in soil planar carries out small scale, contact and mass transfer between enriched nutritive substance, pollutant and oil degradation microbial inoculum, at the same time, the electrochemical oxidation reactions that electrode surface occurs can cooperate with oil degradation microbial inoculum degraded oil type organic, the final oil degradation microbial inoculum that improves improves remediation efficiency to the degradation effect of petroleum type organic.
Description
Technical field
The invention belongs to the technical fields of dystopy microorganism remediation petroleum-type organic material contaminated soil, and in particular to one kind two
Tie up the device and method that AC field enhancement microbiological repairs petroleum-type organic material contaminated soil.
Background technique
Relative to heavy metal pollution of soil, organic contamination is due to complicated components, many kinds of and processing difficulty is bigger.Stone
Oils organic matter is broadly divided into alkane and aromatic hydrocarbons (mononuclear aromatics and polycyclic aromatic hydrocarbon) as a kind of organic pollutant difficult to degrade,
The character of soil will be changed after into soil, fertility and to the crop of the grown on soil and the relevant people and animals of food chain
It causes to seriously endanger.Therefore, it is necessary to find the recovery technique processing petroleum-type organic material contaminated soil of economical and efficient.
Microorganism remediation technology is one of petroleum-type organic material contaminated soil recovery technique common at present, is referred to using micro-
Contaminant degradation poisonous and hazardous in soil is the process of nontoxic product by the growth metabolism effect of biology.Microorganism is repaired
Recovering technology has the characteristics that economic cost is low, without secondary pollution, is at home and abroad widely applied.But due to nutrients in soil
The mass transfer and limits such as matter, pollutant, microorganism remediation technology there is also degradation cycles long, the low problem of remediation efficiency.
Some researches show that electric repairing technique induces the substance in soil by applying DC electric field in soil media
Occur electrokinesis (electro-osmosis, electromigration and electrophoresis), the movement of each substance in reinforced soil improves microorganism to other substances
The probability for contacting and utilizing, to improve microbial degradation efficiency.At the same time, the electrochemical oxidation reactions that electrode surface occurs
Oxidation-degradable some of the contaminants.Therefore, by the way that electric repairing technique and microorganism remediation technology are coupled, micro- life can be strengthened
The repairing effect of object improves remediation efficiency.
Summary of the invention
In view of the deficiencies in the prior art, the purpose of the present invention is to provide a kind of two-dimentional AC fields to strengthen micro- life
The device and method that object repairs petroleum-type organic material contaminated soil, AC field and oil degradation microbial inoculum reparation are coupled.It hands over
Galvanic electricity field is similar to the DC electric field of continuous change direction, can promote various substances (such as ion, organic contamination in soil
Object, microorganism etc.), small scale reciprocating motion is carried out under the action of electrokinesis, enriched nutritive substance, pollutant and petroleum drop
The contact between microbial inoculum and mass transfer are solved, the final oil degradation microbial inoculum that improves is improved and repaired to the degradation effect of petroleum type organic
Efficiency.
Specific technical solution of the present invention is as follows:
The invention discloses the device that a kind of two-dimentional AC field enhancement microbiological repairs petroleum-type organic material contaminated soil,
Including bioreactor, sealing cover with holes, control system, spray system, removable stirring system, removable sampling monitoring system
And guide rail；The middle part of guide rail is arranged in the bioreactor, and bioreactor is square, internal to be used as conversion zone, life
The four sides inner wall of object reactor is equipped with two pairs of sheet DSA electrodes；The sealing cover with holes is arranged at the top of bioreactor, band
Hole sealing cover is equipped with several circular holes；
The spray system is made of storage tank and spray pipeline, and described spray pipeline one end is connected with storage tank, and the other end is worn
The circular hole crossed on sealing cover with holes protrudes into bioreactor；The control system automatically controls system with AC power source and PLC
System, AC power source are used to apply alternating voltages to two pairs of DSA electrodes, and PLC automatic control system is for controlling spray system, removable
Dynamic stirring system and removable sampling monitoring system；The removable stirring system is arranged on guide rail, including the first lifting shaft
With several agitating rollers being connected on the first lifting shaft；The removable sampling monitoring system is arranged on guide rail, including second
Lifting shaft and several samplers being connected on the second lifting shaft.
Preferably, bioreactor four sides inner wall, which reclines, sheet DSA electrode.Sheet DSA electrode can be protected
After card applies voltage, the electric-field intensity distribution in soil is uniform, while having certain electrocatalytic oxidation effect, can cooperate with petroleum
Degradation bacterial agent degradation of contaminant.
Preferably, the slot that the sealing cover with holes is placed with 4 for electrode.
Preferably, the control system includes AC power source and PLC control system.Wherein AC power source by transformer,
AC voltage regulator and voltage and current display composition can be given respectively on two pairs of DSA electrodes and apply stable sinusoidal ac.
PLC automatic control system is used to control the shifting of the spray, removable stirring system and removable sampling monitoring system of spray system
It is dynamic.
Preferably, the spray system is made of storage tank and spray pipeline.Storage tank is respectively used to storage soil addition
Liquid and oil degradation microbial inoculum can control automatic spray by control system.
Preferably, the removable stirring system includes lifting shaft and agitating roller.Agitating roller is solid stainless steel bar,
Agitating roller is inserted into soil by the lifting of lifting shaft and is stirred.The lifting of movement and lifting shaft of the system on guide rail
By the PLC automatic control system control in control system.
Preferably, the removable sampling monitoring system includes lifting shaft and sampler.Sampler is hollow stainless
Rod iron will be inserted into soil by the lifting of lifting shaft and is sampled.Soil sample can be removed out of hollow sampler.System is being led
The lifting of movement and lifting shaft on rail is controlled by the PLC automatic control system in control system.
It is organic using the two-dimentional AC field enhancement microbiological reparation petroleum-type of above-mentioned apparatus that the present invention also provides a kind of
The method of object contaminated soil, includes the following steps:
1) contaminated soil is added into bioreactor, covers sealing cover with holes, is infused by spray system to contaminated soil
Enter soil additive, mobile removable stirring system is located at agitating roller right above the circular hole on sealing cover with holes on guide rail；
The contraction of the first lifting shaft is controlled, agitating roller enters inside bioreactor and is inserted into contaminated soil, utilizes the rotation of agitating roller
It stirs to contaminated soil, to adjust the physicochemical property of contaminated soil；After adjusting, removable stirring system is reset to just
Beginning position；
2) to the oil degradation microbial inoculum after contaminated soil sprinkling activation and stirring system (5) are utilized by spray system (4)
It stirs uniformly；
3) apply sinusoidal ac on two pairs of DSA electrodes (8) respectively using the AC power source in control system (3)；
4) every setting time, the mobile removable sampling monitoring system on guide rail；Sampler is set to be located at sealing cover with holes
On circular hole right above, control the second lifting shaft shrink, sampler, which enters inside bioreactor and is inserted into contaminated soil, to be taken
Sample is used for analysis detection.
The strain of the oil degradation microbial inoculum is commercially available.Above-mentioned oil degradation microbial inoculum is inoculated into glucose culture first
It is activated 3 days in base, the oil degradation microbial inoculum after being activated.The fountain height of oil degradation microbial inoculum after activation is Polluted Soil per ton
0.25-0.5L is sprayed in earth.
Further preferably, soil additive described in step 1) is used to adjust the physicochemical property of soil, including water, nitric acid
Sodium, ammonium sulfate, dipotassium hydrogen phosphate.
Further preferably, the physicochemical property that contaminated soil is adjusted described in step 1) is the moisture content for adjusting contaminated soil
30%~35%, the pH of contaminated soil is adjusted 6~8.Oil degradation microbial inoculum provides suitable soil environment.
Further preferably, the physicochemical property that contaminated soil is adjusted described in step 1) further includes adjusting carbon in contaminated soil:
Nitrogen: the mass ratio of phosphorus is 100: (5~10): 1.It wherein adjusts nitrogen used to be provided by sodium nitrate and ammonium sulfate, phosphorus is by phosphoric acid hydrogen
Dipotassium provides.Therefore nitric acid in soil additive need to be adjusted according to the concentration of organic carbon, available nitrogen and rapid available phosphorus in contaminated soil
The ratio of sodium, ammonium sulfate, dipotassium hydrogen phosphate, wherein the mass ratio of sodium nitrate and ammonium sulfate is preferably 1:1.
Further preferably, AC field frequency described in step 3) is 50Hz, peak strength (the i.e. electricity at electrode both ends
Pressure/electrode spacing) it is 50-100V/m.Due to using sinusoidal ac, so above-mentioned peak strength is sinusoidal ac
Electric field strength when pressure is peak value.The voltage stabilization for keeping power supply to export is needed during this.
Further preferably, the petroleum-type organic pollution is straight-chain hydrocarbons pollution, branched-chain hydrocarbons pollution or aromatic hydrocarbon pollution
One of or it is a variety of.
Compared with prior art, the present invention possessed beneficial effect is:
Compared with traditional bioreactor, the electrokinesis of the two-dimentional AC field induction of application can make the substance in soil
The reciprocating motion of small scale, the contact between enriched nutritive substance, pollutant and oil degradation microbial inoculum are carried out in two-dimensional surface
And mass transfer.At the same time, DSA electrode has stronger electrochemical catalytic oxidation effect, and oil degradation microbial inoculum can be cooperateed with to degrade,
To improve microorganism remediation efficiency, economy and time cost are saved.
Detailed description of the invention
Fig. 1 is the schematic diagram for the device that AC field enhancement microbiological repairs petroleum-type organic material contaminated soil；
Fig. 2 is the structural schematic diagram of bioreactor；
Fig. 3 is the structural schematic diagram of sealing cover with holes；
In figure: bioreactor -1, sealing cover -2 with holes, control system -3, spray system -4 move stirring system -
5, move sampling monitoring system -6, guide rail -7, DSA electrode -8, storage tank -9, spray pipeline -10, lifting shaft -11, agitating roller -
12, sampler -13.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, so that this field ground technology
Personnel can better understand advantages and features of the invention, to make apparent boundary to protection scope of the present invention
It is fixed.Example described in the invention only makes a part of the embodiment of the present invention, instead of all the embodiments.For this field
For technical staff, it can still modify to aforementioned documented technical solution, or to part of technical characteristic
It is replaced on an equal basis.Based on the example in the present invention, those of ordinary skill in the art are in the premise for not making creative work
Under other embodiments obtained, shall fall within the protection scope of the present invention.
Build the dress that two-dimentional AC field enhancement microbiological as shown in Figs. 1-3 repairs petroleum-type organic material contaminated soil
It sets, including bioreactor 1, sealing cover with holes 2, control system 3, spray system 4, removable stirring system 5, removable sampling
Monitoring system 6 and guide rail 7.
1 four wall of bioreactor, which reclines, sheet DSA electrode 8, it is ensured that after applying voltage, the electric-field strength in soil
Degree is evenly distributed, while having certain electrocatalytic oxidation effect, can cooperate with oil degradation microbial inoculum degradation of contaminant.
Sealing cover band 2 with holes has 4 slots placed for electrode and 4 for spraying, stirring or sampling monitoring
Circular hole.
Control system 3 includes AC power source and PLC control system, is integrated in a cabinet.Wherein AC power source is by becoming
Depressor, AC voltage regulator and voltage and current display, for applying sinusoidal ac to two pairs of DSA electrodes 8, ac frequency is
50Hz.PLC automatic control system is for controlling spray system 4, removable stirring system 5 and removable sampling monitoring system 6.
Spray system 4 includes two sets of storage tanks 9 and spray pipeline 10, is respectively used to spray soil additive and oil degradation bacteria
Agent can control automatic spray by control system 3.Soil additive for adjusting soil physico-chemical property, including water, sodium nitrate,
Ammonium sulfate, dipotassium hydrogen phosphate.Oil degradation microbial inoculum is bought from the market, and above-mentioned oil degradation microbial inoculum is inoculated into grape first
It is activated 3 days in sugar culture-medium, the oil degradation microbial inoculum after being activated is spare.0.25-0.5L stone is sprayed in contaminated soil per ton
Oily degradation bacterial agent.
Removable stirring system 5 includes lifting shaft 11 and agitating roller 12.Agitating roller 12 is solid stainless steel bar, passes through lifting
Axis 11 is inserted into soil and is stirred.System movement and lifting shaft 11 on guide rail 7 lifting by control system 3
PLC automatic control system control.
Removable sampling monitoring system 6 includes lifting shaft 11 and sampler 13.Sampler 13 is hollow stainless steel bar, is passed through
Lifting shaft 11 is inserted into soil and is sampled.Soil sample can be removed out of hollow sampler.Movement and liter of the system on guide rail 7
The lifting for dropping axis 11 is controlled by the PLC automatic control system in control system 3.
Embodiment 1
Oil pollution soil is taken, is added in bioreactor after crossing 20 meshes, spray soil additive adjusts soil physical chemistry
Property adjusts the moisture content of soil 30%~35%, and the pH of soil is in 6~8 ranges.According to organic carbon in soil, quick-acting
The concentration of nitrogen and rapid available phosphorus adjusts carbon: nitrogen: phosphorus ratio 100: 5: 1, and wherein nitrogen is provided by sodium nitrate and ammonium sulfate, and phosphorus is by phosphorus
Sour hydrogen dipotassium provides.
Uniformly sprayed into contaminated soil it is activated after oil degradation microbial inoculum, stir uniformly after respectively in bioreactor
In two pairs of sheet DSA electrodes on apply sinusoidal ac, electric field frequency 50Hz, crest voltage intensity is 100V/m.Electric field
Apply after two weeks, it is 29.8% that sampling, which measures contaminant degradation rate,.
Embodiment 2
Gasoline contamination soil is taken, is added in bioreactor after crossing 20 meshes, spray soil additive adjusts soil physical chemistry
Property adjusts the moisture content of soil 30%~35%, and the pH of soil is in 6~8 ranges.According to organic carbon in soil, quick-acting
The concentration of nitrogen and rapid available phosphorus adjusts carbon: nitrogen: phosphorus ratio 100: 10: 1, and wherein nitrogen is provided by sodium nitrate and ammonium sulfate, and phosphorus is by phosphorus
Sour hydrogen dipotassium provides.
Uniformly sprayed into contaminated soil it is activated after oil degradation microbial inoculum, stir uniformly after respectively in bioreactor
In two pairs of sheet DSA electrodes on apply sinusoidal ac, electric field frequency 50Hz, crest voltage intensity is 50V/m.Electric field is applied
Add after two weeks, it is 31.6% that sampling, which measures contaminant degradation rate,.
The following are the experimental results for not applying AC field in bioreactor:
Comparative example 1
Oil pollution soil is taken, is added in bioreactor after crossing 20 meshes, spray soil additive adjusts soil physical chemistry
Property adjusts the moisture content of soil 30%~35%, and the pH of soil is in 6~8 ranges.According to organic carbon in soil, quick-acting
The concentration of nitrogen and rapid available phosphorus adjusts carbon: nitrogen: phosphorus ratio 100: 5: 1, and wherein nitrogen is provided by sodium nitrate and ammonium sulfate, and phosphorus is by phosphorus
Sour hydrogen dipotassium provides.
Uniformly sprayed into contaminated soil it is activated after oil degradation microbial inoculum, stir uniformly after start to repair.Repair two
Zhou Hou, it is 12.8% that sampling, which measures contaminant degradation rate,.
Comparative example 2
Gasoline contamination soil is taken, is added in bioreactor after crossing 20 meshes, spray soil additive adjusts soil physical chemistry
Property adjusts the moisture content of soil 30%~35%, and the pH of soil is in 6~8 ranges.According to organic carbon in soil, quick-acting
The concentration of nitrogen and rapid available phosphorus adjusts carbon: nitrogen: phosphorus ratio 100: 5: 1, and wherein nitrogen is provided by sodium nitrate and ammonium sulfate, and phosphorus is by phosphorus
Sour hydrogen dipotassium provides.
Uniformly sprayed into contaminated soil it is activated after oil degradation microbial inoculum, stir uniformly after start to repair.Repair two
Zhou Hou, it is 14.5% that sampling, which measures contaminant degradation rate,.
By comparing contaminant degradation degree effect, the present invention strengthens the reparation of oil degradation microbial inoculum using two-dimentional AC field,
It is organic to petroleum-type that the electrokinesis and electrochemical oxidation process generated using AC field induction strengthens oil degradation microbial inoculum
The degradation effect of species organic matter.The electrokinesis of two-dimentional AC field induction can promote in soil substance in two-dimensional surface
Small scale moves back and forth, contact and mass transfer between enriched nutritive substance, pollutant and oil degradation microbial inoculum, and electrode surface occurs
Electrochemical oxidation reactions can cooperate with oil degradation microbial inoculum degraded oil type organic, the final oil degradation microbial inoculum that improves is to petroleum
The degradation effect of type organic improves remediation efficiency.
Claims (10)
1. the device that a kind of two dimension AC field enhancement microbiological repairs petroleum-type organic material contaminated soil, it is characterised in that: packet
Bioreactor (1) is included, sealing cover with holes (2), control system (3), spray system (4), stirring system (5) is moved, is removable
Dynamic sampling monitoring system (6) and guide rail (7)；
The bioreactor (1) is arranged at the middle part of guide rail (7), and bioreactor (1) is square, internal to be used as reaction zone
The four sides inner wall in domain, bioreactor (1) is equipped with two pairs of sheet DSA electrodes (8)；
At the top of bioreactor (1), sealing cover (2) with holes is equipped with several circular holes for sealing cover with holes (2) setting；
The control system (3) includes AC power source and PLC automatic control system, and AC power source is used for two pairs of sheet DSA electricity
Pole (8) applies sinusoidal ac, and PLC automatic control system is for controlling spray system (4), removable stirring system (5) and can
Mobile sampling monitoring system (6)；
The spray system (4) is made of storage tank (9) and spray pipeline (10), described spray pipeline (10) one end and storage tank (9)
It is connected, the circular hole that the other end passes through on sealing cover with holes (2) protrudes into bioreactor (1)；
The one end of removable stirring system (5) setting in guide rail (7)；Including the first lifting shaft and several it is connected to first liter
Agitating roller (12) on axis drops；
The other end of removable sampling monitoring system (6) setting in guide rail (7)；Including the second lifting shaft and several it is connected to
Sampler (13) on second lifting shaft.
2. the dress that two dimension AC field enhancement microbiological according to claim 1 repairs petroleum-type organic material contaminated soil
Set, which is characterized in that the lifting of movement and first lifting shaft of the removable stirring system (5) on guide rail (7) by
PLC automatic control system control in control system (3)；Agitating roller (12) is solid stainless steel bar.
3. the dress that two dimension AC field enhancement microbiological according to claim 1 repairs petroleum-type organic material contaminated soil
It sets, which is characterized in that the liter of movement and second lifting shaft of the removable sampling monitoring system (6) on guide rail (7)
Drop is controlled by the PLC automatic control system in control system (3)；Sampler (13) is hollow stainless steel bar.
4. a kind of AC field enhancement microbiological for applying device as described in claim 1 repairs petroleum-type organic material contaminated soil
Method, include the following steps:
1) to contaminated soil is added in bioreactor (1), sealing cover with holes (2) are covered, by spray system (4) to Polluted Soil
Earth injects soil additive, on guide rail (7) mobile removable stirring system (5), agitating roller (12) is made to be located at sealing cover with holes
(2) right above the circular hole on；The contraction of the first lifting shaft is controlled, agitating roller (12) enters bioreactor (1) inside and is inserted into dirt
It contaminates in soil, is stirred using the rotation of agitating roller (12) to contaminated soil, to adjust the physicochemical property of contaminated soil；It adjusts
After, removable stirring system (5) are reset to initial position；
2) it is stirred to the oil degradation microbial inoculum after contaminated soil sprinkling activation and by spray system (4) using stirring system (5)
Uniformly；
3) apply sinusoidal ac on two pairs of DSA electrodes (8) respectively using the AC power source in control system (3)；
4) every setting time, on guide rail (7) mobile removable sampling monitoring system (6)；It is located at sampler (13) with holes
Right above circular hole on sealing cover (2), the second lifting shaft of control is shunk, and it is internal simultaneously that sampler (13) enters bioreactor (1)
It is inserted into sampling in contaminated soil, is used for analysis detection.
5. the method that AC field enhancement microbiological according to claim 4 repairs petroleum-type organic material contaminated soil,
Be characterized in that, soil additive described in step 1) is used to adjust the physicochemical property of soil, including water, sodium nitrate, ammonium sulfate,
Dipotassium hydrogen phosphate.
6. the method that AC field enhancement microbiological according to claim 4 repairs petroleum-type organic material contaminated soil,
It is characterized in that, the physicochemical property that contaminated soil is adjusted described in step 1) is to adjust soil moisture content 30%~35%, is adjusted
The pH of contaminated soil is saved 6~8.
7. the method that AC field enhancement microbiological according to claim 6 repairs petroleum-type organic material contaminated soil,
It is characterized in that, the physicochemical property that contaminated soil is adjusted described in step 1) further includes adjusting carbon in soil: nitrogen: the mass ratio of phosphorus is
100: (5~10): 1, wherein adjust nitrogen used and provided by sodium nitrate and ammonium sulfate, phosphorus is provided by dipotassium hydrogen phosphate, wherein nitre
The mass ratio of sour sodium and ammonium sulfate is 1:1.
8. the method that AC field enhancement microbiological according to claim 4 repairs petroleum-type organic material contaminated soil,
Be characterized in that, the oil degradation microbial inoculum after activation described in step 2) the preparation method comprises the following steps: oil degradation microbial inoculum is inoculated into
It is activated 3 days in dextrose culture-medium, the oil degradation microbial inoculum after being activated；Oil degradation after activation described in step 2)
The fountain height of microbial inoculum is to spray 0.25-0.5L in contaminated soil per ton.
9. the method that AC field enhancement microbiological according to claim 4 repairs petroleum-type organic material contaminated soil,
Be characterized in that: AC field frequency described in step 3) is 50 Hz, peak strength 50-100V/m.
10. the method that AC field enhancement microbiological according to claim 4 repairs petroleum-type organic material contaminated soil,
Be characterized in that, the petroleum-type organic pollution be straight-chain hydrocarbons pollution, branched-chain hydrocarbons pollution or aromatic hydrocarbon pollution one of or
It is a variety of.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111054742A (en) * | 2019-12-30 | 2020-04-24 | 浙江大学 | Device and method for reinforcing microbial remediation of petroleum organic matter contaminated soil by direct current-alternating current coupling electric field |
| CN112881131A (en) * | 2021-02-24 | 2021-06-01 | 武汉理工大学 | Soil sample water content preparation instrument and working method thereof |
| WO2021121339A1 (en) * | 2019-12-17 | 2021-06-24 | 广东以色列理工学院 | Soil heavy metal remediation apparatus |
| CN115055502A (en) * | 2022-06-09 | 2022-09-16 | 浙江大学衢州研究院 | Device and method for restoring organic matter contaminated soil through enhanced leaching by direct current-alternating current coupling electric field |
| CN116022903A (en) * | 2022-12-30 | 2023-04-28 | 河北工业大学 | Method for restoring underground water by coupling chemical oxidation of single-phase alternating-current electrode array |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5458747A (en) * | 1994-01-21 | 1995-10-17 | Electrokinetics, Inc. | Insitu bio-electrokinetic remediation of contaminated soils containing hazardous mixed wastes |
| WO1996041689A2 (en) * | 1995-06-08 | 1996-12-27 | Geo-Kinetics International Inc. | Electrochemically-aided biodigestion of organic materials |
| CN103160448A (en) * | 2011-12-13 | 2013-06-19 | 中国科学院沈阳应用生态研究所 | Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof |
| CN203196987U (en) * | 2013-04-02 | 2013-09-18 | 中国人民解放军后勤工程学院 | Anaerobic-aerobiotic compatible biological slurry reactor |
| CN203602605U (en) * | 2013-11-30 | 2014-05-21 | 邴欣 | Laboratory microalgae cultivation reactor |
| US20150104855A1 (en) * | 2013-10-12 | 2015-04-16 | Nankai University | Enhanced combination method for phytoremediation of petroleum contaminated soil by Impatiens balsamina L. associated with the indigenous microorganisms |
| CN105771821A (en) * | 2016-03-24 | 2016-07-20 | 张洪美 | Air and liquid reaction parallel test tank |
| CN205869062U (en) * | 2016-07-20 | 2017-01-11 | 河南工程学院 | Soil heavy metal electrokinetic remediation experimental system |
| CN107570532A (en) * | 2017-10-17 | 2018-01-12 | 陆隽鹤 | A kind of method of electronic diffusion electrical heating coupling rehabilitating soil organic contamination |
| CN207019928U (en) * | 2017-07-07 | 2018-02-16 | 苏州汉宣检测科技有限公司 | One kind is used for soil sample, detection integrated device |
| CN207379744U (en) * | 2017-10-31 | 2018-05-18 | 赵群 | A kind of sampler for subterranean coal exploration |
| CN108447376A (en) * | 2018-02-12 | 2018-08-24 | 中国科学院南京土壤研究所 | A kind of assessment nano-particle migrates in underground environment and returns the analogue experiment installation to become and method |
| CN109317511A (en) * | 2018-12-06 | 2019-02-12 | 中电建路桥集团有限公司 | The device and method of AC field enhancement microbiological reparation petroleum hydrocarbon contaminated soil |
| CN210411897U (en) * | 2019-05-21 | 2020-04-28 | 中电建路桥集团有限公司 | Device for reinforcing microbial remediation of petroleum organic matter contaminated soil by two-dimensional alternating current electric field |
-
2019
- 2019-05-21 CN CN201910425170.7A patent/CN110193512B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5458747A (en) * | 1994-01-21 | 1995-10-17 | Electrokinetics, Inc. | Insitu bio-electrokinetic remediation of contaminated soils containing hazardous mixed wastes |
| WO1996041689A2 (en) * | 1995-06-08 | 1996-12-27 | Geo-Kinetics International Inc. | Electrochemically-aided biodigestion of organic materials |
| CN103160448A (en) * | 2011-12-13 | 2013-06-19 | 中国科学院沈阳应用生态研究所 | Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof |
| CN203196987U (en) * | 2013-04-02 | 2013-09-18 | 中国人民解放军后勤工程学院 | Anaerobic-aerobiotic compatible biological slurry reactor |
| US20150104855A1 (en) * | 2013-10-12 | 2015-04-16 | Nankai University | Enhanced combination method for phytoremediation of petroleum contaminated soil by Impatiens balsamina L. associated with the indigenous microorganisms |
| CN203602605U (en) * | 2013-11-30 | 2014-05-21 | 邴欣 | Laboratory microalgae cultivation reactor |
| CN105771821A (en) * | 2016-03-24 | 2016-07-20 | 张洪美 | Air and liquid reaction parallel test tank |
| CN205869062U (en) * | 2016-07-20 | 2017-01-11 | 河南工程学院 | Soil heavy metal electrokinetic remediation experimental system |
| CN207019928U (en) * | 2017-07-07 | 2018-02-16 | 苏州汉宣检测科技有限公司 | One kind is used for soil sample, detection integrated device |
| CN107570532A (en) * | 2017-10-17 | 2018-01-12 | 陆隽鹤 | A kind of method of electronic diffusion electrical heating coupling rehabilitating soil organic contamination |
| CN207379744U (en) * | 2017-10-31 | 2018-05-18 | 赵群 | A kind of sampler for subterranean coal exploration |
| CN108447376A (en) * | 2018-02-12 | 2018-08-24 | 中国科学院南京土壤研究所 | A kind of assessment nano-particle migrates in underground environment and returns the analogue experiment installation to become and method |
| CN109317511A (en) * | 2018-12-06 | 2019-02-12 | 中电建路桥集团有限公司 | The device and method of AC field enhancement microbiological reparation petroleum hydrocarbon contaminated soil |
| CN210411897U (en) * | 2019-05-21 | 2020-04-28 | 中电建路桥集团有限公司 | Device for reinforcing microbial remediation of petroleum organic matter contaminated soil by two-dimensional alternating current electric field |
Non-Patent Citations (2)
| Title |
|---|
| 李婷婷;张玲妍;郭书海;黄殿男;: "完全对称电场对电动-微生物修复石油污染土壤的影响", 环境科学研究, no. 10, 15 October 2010 (2010-10-15) * |
| 陈峰;: "高密度电法在污染场地调查中的应用研究", 环境与发展, no. 07, 28 July 2018 (2018-07-28) * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021121339A1 (en) * | 2019-12-17 | 2021-06-24 | 广东以色列理工学院 | Soil heavy metal remediation apparatus |
| EP3936247A4 (en) * | 2019-12-17 | 2022-07-06 | Guangdong Technion Israel Institute of Technology | Soil heavy metal remediation apparatus |
| CN111054742A (en) * | 2019-12-30 | 2020-04-24 | 浙江大学 | Device and method for reinforcing microbial remediation of petroleum organic matter contaminated soil by direct current-alternating current coupling electric field |
| CN111054742B (en) * | 2019-12-30 | 2021-03-23 | 浙江大学 | Device and method for repairing petroleum organic matter contaminated soil |
| CN112881131A (en) * | 2021-02-24 | 2021-06-01 | 武汉理工大学 | Soil sample water content preparation instrument and working method thereof |
| CN112881131B (en) * | 2021-02-24 | 2024-01-26 | 武汉理工大学 | Soil sample water content preparation instrument and working method thereof |
| CN115055502A (en) * | 2022-06-09 | 2022-09-16 | 浙江大学衢州研究院 | Device and method for restoring organic matter contaminated soil through enhanced leaching by direct current-alternating current coupling electric field |
| CN115055502B (en) * | 2022-06-09 | 2024-03-19 | 浙江大学衢州研究院 | Device and method for restoring organic matter polluted soil by direct current-alternating current coupling electric field enhanced leaching |
| CN116022903A (en) * | 2022-12-30 | 2023-04-28 | 河北工业大学 | Method for restoring underground water by coupling chemical oxidation of single-phase alternating-current electrode array |
| CN116022903B (en) * | 2022-12-30 | 2024-07-23 | 河北工业大学 | Method for restoring underground water by coupling chemical oxidation of single-phase alternating-current electrode array |
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