CN110186884A - A kind of visualization molecular engram nano-sensor and its preparation and application - Google Patents
A kind of visualization molecular engram nano-sensor and its preparation and application Download PDFInfo
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- CN110186884A CN110186884A CN201910425077.6A CN201910425077A CN110186884A CN 110186884 A CN110186884 A CN 110186884A CN 201910425077 A CN201910425077 A CN 201910425077A CN 110186884 A CN110186884 A CN 110186884A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention belongs to material, analytical chemistry and field of food safety, and in particular to a kind of molecular engram ratio fluorescent nano sensor based on double fluorescent emissions and preparation method thereof and the application for folic acid in Visual retrieval food.Visualizing molecular engram nano-sensor is to polymerize trace in Nano particles of silicon dioxide surface sol-gel by one-step method, embed red fluorescence cadmium telluride quantum dot (CdTe QDs) and the folic acid with spontaneous blue-fluorescence, using the hole for eluting folic acid as recognition site, double fluorescent emissive molecules trace nano-sensors with core-shell structure are obtained.It is simpler that preparation method of the present invention compared with conventional two-step prepares molecular engram ratio fluorescent sensor, avoids cumbersome synthesis step, shortens experimental period.In addition, the sensor that is prepared of the method for the present invention can highly sensitive, high selection, self-correcting normal incidence detection folic acid, and fluorescence color abundant is provided and is developed, realize the Visual retrieval to object.
Description
Technical field
The invention belongs to material, analytical chemistry and field of food safety, and in particular to a kind of point based on double fluorescent emissions
Sub- trace ratio fluorescent sensor and preparation method thereof and application for folic acid in Visual retrieval food.
Background technique
Folic acid is a kind of water soluble vitamin, there is important clinical meaning to human health.It is as important coenzyme
The factor plays important physiological action in cell metabolism, participates in nucleic acid and red blood cell is formed, cellular replication and tissue are given birth to
It is long.In addition, folic acid, as a kind of diet hardening agent, current adult daily recommended intake in the U.S.'s is 400 μ g.Pregnant woman lacks leaf
It is sour then Fetal neurotubules malformation can be caused；Folic acid intake excessively then will affect the absorption of other nutriments, such as B12, zinc, thus
Cause other health problems.With being gradually increased for people's Consciousness of food security, developing one kind can be quick, convenient, reliably
The method for detecting folate content in food is extremely urgent.
Molecular imprinting technology refers to the principle combined using Ag-Ab, prepares to specific target molecules with single-minded
Polymer, that is, molecularly imprinted polymer (MIPs) of recognition performance.Compared with the antibody equally with specific recognition function,
MIPs possesses the advantages such as higher physical and chemical stability, at low cost, easily prepared, and has high precordainment, strong identification
The features such as property, wide applicability.Fluorescence nano sensing has the advantage highly sensitive, easy to operate, the response time is short, has
Conducive to the detection of trace materials.In recent years, molecular imprinting technology and fluorescence detection have caused the great interest of researcher, obtain
Fast development.Then, in conjunction with the high specific of the two and high sensitivity, it is desirable to molecular engram fluorescence sense is prepared, it will
Its quick visualization detection for being applied to nutrition fortifier in complex matrices.However, conventional molecular trace fluorescence sense only has one
A emission peak, the interference (such as: detection substrate, instrument fluctuation, photobleaching) vulnerable to other various uncorrelated factors and limit
The detection of its accurate quantification；In addition, the change with target concentration can only often cause the variation of its fluorescent brightness, visually
More direct color change cannot be provided.
Ratio fluorescent sensing is then the change by comparing the fluorescence intensity of two kinds of different illuminators before and after analyte is added
Change to realize the purpose for testing and analyzing object.The basic principle is that: analysis substrate can cause the fluorescence intensity at a wavelength to become
Change (response signal), and the fluorescence intensity at another wavelength remains unchanged or generates the variation opposite with response signal, then
Correlativity is established between the concentration of analysis substrate according to two changing for peak intensity ratio, to carry out to analysis substrate
Quantitative detection.This interference that can not only weaken foeign element improves the reliability of analysis, also tends to that fluorescence intensity ratio can be passed through
Variation show the variation of macroscopic fluorescence color, therefore open hole detection will become one kind in molecular imprinting technology can
Energy.However, the synthesis process of the ratio fluorescent sensing of hud typed molecular engram is cumbersome at present, two steps are usually directed to, it first will ginseng
It nuzzles Nano particles of silicon dioxide than fluorescence packets, then carries out trace on its surface, is i.e. " trace after first embedding "；Or first distinguish
It prepares hud typed single-shot to penetrate molecular engram fluorescence sense and embed the Nano particles of silicon dioxide of reference fluorescent, i.e., " first prepares
It mixes afterwards ".
Summary of the invention
In view of the deficiencies of the prior art, the molecule print based on one kettle way that the problem to be solved by the invention is to provide a kind of
The application of mark ratio fluorescent nano sensing and its preparation and Visual retrieval folic acid.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of visualization molecular engram nano-sensor, visualization molecular engram nano-sensor are to be existed by one-step method
Nano particles of silicon dioxide surface sol-gel polymerize trace, embeds red fluorescence cadmium telluride quantum dot (CdTe QDs) and has
There is the folic acid of spontaneous blue-fluorescence, using the hole for eluting folic acid as recognition site, obtains double fluorescent emissions with core-shell structure
Folic acid trace microballoon.
Gained sensing has double transmitting fluorescence, and under 365 excitation wavelengths, transmitting range is respectively 440-460nm
And 610-620nm.
A kind of preparation method visualizing molecular engram nano-sensor, the solution of Xiang Hanyou Nano particles of silicon dioxide
Middle addition red fluorescence cadmium telluride quantum dot (CdTe QDs), folic acid and 3-aminopropyltriethoxysilane (APTES), are stirred
1-2h is mixed, ethyl orthosilicate (TEOS) and ammonium hydroxide are continuously added after mixing and carries out sol-gel polymerization reaction under dark surrounds
10-12 hours, product is precipitated after reaction with centrifugal process, abandons supernatant, elute the leaf in sediment repeatedly with 24mL methanol
Acid obtains the folic acid trace microballoon with core-shell structure, and is scattered in 6mL ultrapure water again.
The total volume of the sol-gel polymerizations reaction system is controlled in 20-22mL；Wherein, silica dioxide nano particle
Sub- solution, CdTe QDs, folic acid, APTES, TEOS, ammonia volume are respectively 1-2mL, 3-5mL, 8-10mL, 37-75 μ L, 50-
100μL、50-100μL.
The Nano particles of silicon dioxide solution is that ammonium hydroxide is added in Xiang Shui/ethyl alcohol mixed solution, stirs 10-
The ethanol solution of ethyl orthosilicate (TEOS) is drop by drop added in 30min with constant pressure funnel after mixing, stirring
10-12h is reacted, liquid is discarded supernatant after the product centrifugation of acquisition, ethanol washing precipitating is finally scattered in 50mL ultrapure water again
In, for use.
The concentration of the Nano particles of silicon dioxide is 1.2-1.5mg/mL；The total volume of the water/alcohol mixed solution
For 80-100mL, volume ratio 5:3；Volume in the TEOS ethanol solution is 25-30mL, wherein the volume accounting of TEOS
For 2%-3%.
The concentration of the folic acid is 1-1.2g/L；The concentration of the NaOH ethanol solution are as follows: 0.01-0.02 molL-1,
Volume fraction shared by ethyl alcohol is 20-30%.
A kind of application visualizing molecular engram nano-sensor, the sensor are detecting food and biological sample,
The application of nutrition fortifier folic acid in (serum).
The sensor is in prepare liquid, and signal, fluorescence are strong in response by the red fluorescence CdTe QDs in sensor
Degree is reduced with the increase of target concentration, and itself blue-fluorescence of object folic acid gradually increases, and is sent out by difference at two
The variation of peak intensity ratio is penetrated, to realize the visualization open hole detection to folic acid.
The invention has the benefit that
1) present invention constructs hud typed molecular engram ratio fluorescent sensing using one-step method sol-gel polymerizations for the first time,
Synthesis process is simplified while improving mass transfer rate, avoids multi-stage synthesis molecularly imprinted polymer, shortens experiment week
Phase reduces experiment and spends.
2) inventive sensor quenches red CdTe QDs fluorescence and then the specific recognition to folic acid；Further
It says in the detection, photo induced electron transfer occurs between red CdTe QDs and analyte folic acid, with the increase of folic acid concentration,
Red CdTe QDs fluorescence gradually quenches, and folic acid itself blue-fluorescence gradually increases, thus realize ratio test folic acid, and
Change fluorescence color from red-pinkish-purple to blue, realizes the Visual retrieval of folic acid.
3) inventive sensor has given full play to the highly sensitive of the highly selective and ratio fluorescent technology of molecular engram polymerization
Property, self-correcting, the advantages such as anti-interference, developed detection of a kind of convenient and efficient and reliable quantitative approach for folic acid, and mention
Qualitative detection is used for for color change abundant；This sensor detection folic acid range be 0.23-113 μM, detection line down to
4.8nM；This sensor overcomes the disadvantages of traditional single fluorescent optical sensor is vulnerable to interfering, being unstable, seeks in food and other field
Supporting has significant application value in the Detection & Controling of hardening agent.
Detailed description of the invention
Fig. 1 is the preparation process schematic diagram for the visualization molecular engram nano-sensor that the embodiment of the present invention is mentioned.
Fig. 2 is the Nano particles of silicon dioxide (SiO that the embodiment of the present invention is mentioned2), folic acid trace ratio fluorescent sensor
(FL-MIPs) with non-trace ratio fluorescent sensor (FL-NIPs) shape appearance figure.
Fig. 3 is the folic acid trace ratio fluorescent sensor (FL-MIPs) mentioned of the embodiment of the present invention in different amounts condition
It is lower detection folic acid fluorescence spectra and fluorescence color develop, wherein the dosage of FL-MIPs from A-E be respectively 50 μ L, 100 μ
L,125μL,150μL。
Fig. 4 A, C are respectively the folic acid trace ratio fluorescent sensor (FL-MIPs) and non-print that the embodiment of the present invention is mentioned
Mark ratio fluorescent sensor (FL-NIPs) detects the fluorescence spectra of folic acid and fluorescence color changes.B, D are then respective correspondence
Fluorescence intensity ratio variation and the fit correlation figure of folic acid concentration.
Fig. 5 is folic acid trace ratio fluorescent sensor (FL-MIPs) and non-trace ratio fluorescent sensor (FL-NIPs)
Identify fluorescence intensity ratio and fluorescence color variation after different analogs, each material concentration is 20mg L-1.Interior illustration is then
In various concentration than ninopterin/folic acid (CMTX/CFA) under the conditions of folic acid trace ratio fluorescent sensor (FL-MIPs) fluorescence
Intensity rate variation.
Specific embodiment
Explanation that the present invention will be further explained with reference to the accompanying drawings and examples.
Inventive sensor shows that transmitting range is 440-460nm and 610- under the excitation wavelength of 365nm
620nm respectively corresponds itself blue-fluorescence of folic acid and the red fluorescence of CdTe QDs, the former is as reference fluorescent, the latter
Fluorescence in response, the detection for folic acid.In optimal conditions, pass through the fluorescence light of fluorescent spectrophotometer assay solution
Spectrum reads emission peak peak intensity, corresponding relationship existing for the variation of calculating fluorescence intensity ratio and folic acid concentration, to carry out leaf
The quantitative inspection of acid.In addition, red fluorescence gradually quenches, and blue autofluorescence gradually increases when the sensor detects folic acid,
To make fluorescence color gradually become blue from red, it can be used for the Visual retrieval of folic acid.Sensor energy prepared by the present invention
Enough high selections detect folic acid with sensitivity, and provide color differentiation and self-correcting function；And easy one kettle way constructs the core
The method of shell mould sensor avoids cumbersome synthesis process while improving mass transfer rate, substantially reduces experiment week
Phase has saved experiment fees, is more broadly applicable to the detection of various nutrition fortifiers.
Embodiment 1
A kind of preparation method (referring to Fig. 1) visualizing molecular engram nano-sensor, steps are as follows:
(1)1g·L-1The preparation of folic acid solution: 0.01molL is first configured-1Sodium hydroxide ethanol solution, to 500mL second
200mg sodium hydroxide is added in alcoholic solution (volume fraction 20%).The 100mL solution is taken, 100mg folic acid is added, just obtains
1g·L-1Folic acid solution
(2) sol-gel method prepares folic acid trace ratio fluorescent sensor (FL-MIPs): to 1mL silica nanometer
8mL ultrapure water, 37 μ LAPTES, above-mentioned folic acid 8mL are added in particle solution to connect after stirring 1 hour formation pre-polymeric mixture
Addition 50 μ LTEOS and 50 μ L ammonium hydroxide, reacted 10-12 hours under dark surrounds.After reaction, product is centrifugated
(8000rpm, 10 minutes), discards supernatant liquid, then elutes the template molecule folic acid in precipitating repeatedly with 24mL methanol, obtains
The ratio fluorescent nano of hud typed folic acid trace senses, and is scattered in again spare in 6mL water.Non- trace ratio fluorescent simultaneously
Sensor (FL-NIPs) is prepared using above-mentioned identical method as control, is not only added template molecule leaf in preparation process
Acid.
Application examples 2
1) 100 μ L above-described embodiments is taken to obtain Nano particles of silicon dioxide, folic acid trace ratio fluorescent sensor respectively
(FL-MIPs) and non-trace ratio fluorescent sensor (FL-NIPs) solution is diluted 1000 times respectively, then disperses respectively
On the copper mesh by ethyl alcohol cleaning, after dry, the copper mesh for being loaded with substance after above-mentioned each dilution is seen with transmission electron microscope
It examines；(upper layer referring to fig. 2)
2) 100 μ L above-described embodiments is taken to obtain Nano particles of silicon dioxide, folic acid trace ratio fluorescent sensor respectively
(FL-MIPs) and non-trace ratio fluorescent sensor (FL-NIPs) solution is diluted 100 times respectively, is then dispersed in respectively
On silicon wafer by ethyl alcohol cleaning, after dry, the silicon wafer for being loaded with substance after above-mentioned each dilution is seen with scanning electron microscope
It examines；(lower layer referring to fig. 2)
Fig. 2 is respectively Nano particles of silicon dioxide, folic acid trace ratio fluorescent sensor, non-trace ratio fluorescent sensing
Device, as seen from the figure, the average diameter of Nano particles of silicon dioxide is about 75-85nm, folic acid trace ratio fluorescent sensor,
Non- trace ratio fluorescent sensor is not distinguished significantly in morphology and size, they have coarse surface, average grain
Diameter is about 90nm.Then shell thickness known to calculating is about 5-8nm, and the site positioned at imprinting surface is improved to template
The accessibility of molecule folic acid, and very thin trace shell is conducive to the quick identification to target molecule.
Application examples 3
The folic acid trace ratio for taking above-described embodiment of different volumes 50 μ L, 100 μ L, 125 μ L, 150 μ L to obtain respectively is glimmering
Optical sensor (FL-MIPs) is separately added into the folic acid of various concentration, and wherein the concentration of folic acid is followed successively by 0,1,5,10,20,30,
40, and 50mg L-1, it then is settled to 1mL with ultrapure water, after mixing, 10min is reacted, then uses sepectrophotofluorometer
Measure the fluorescence spectrum (excitation wavelength: 365nm, slit width 5/5nm) of each sample.
It is 50 μ L, 100 μ L, 125 μ L, the FL-MIPs of 150 μ L as Fig. 3 A-E respectively corresponds volume, as seen from the figure, with
The increase of folic acid concentration, red fluorescence are quenched, blue-fluorescence enhancing.As schemed shown in A, when the dosage of FL-MIPs is 50 μ L, solution
Color, which becomes purple again from initial red, becomes blue, and in the majority with blue；With the increase of FL-MIPs volume, red is slightly
It is micro- to increase, but still with red Lan Weizhu (such as figure B)；But excessive FL-MIPs can then cover blue-fluorescence, then in the majority (such as with red
Scheme D and E).Therefore, these four situations can all reduce the variation range of fluorescence color.When FL-MIPs dosage volume is 125 μ L
When, as folic acid concentration increases to 50mg L from 0-1, the fluorescence color variation of sample is red-pink colour-purple-blue, and
It is uniformly distributed.
Application examples 4
The folic acid trace ratio fluorescent sensor (FL-MIPs) and non-trace ratio for taking 125 μ L above-described embodiments to obtain are glimmering
Light sensing (FL-NIPs) is separately added into the folic acid of various concentration, is then settled to 1mL with ultrapure water, wherein the concentration of folic acid according to
Secondary is 0.1,1,5,10,15,20,25,30,35,40,45 and 50mg L-1, after mixing, 10min is reacted, fluorescence is then used
The fluorescence spectrum (excitation wavelength: 365nm, slit width 5/5nm) of each sample of spectrophotometric determination.
As shown in Figure 4 A, under the excitation of 365nm wavelength, two fluorescence of molecular engram ratio fluorescent nano sensor emission
Peak is respectively in 449 and 617nm.With the enhancing of folic acid concentration, red fluorescence intensity is gradually decreased, and blue-fluorescence intensity by
It is cumulative strong, fluorescence color variation is directly resulted in by red and is gradually transitions blue, can be visually seen differentiable, obvious
Color change, thus Visual retrieval folic acid.As shown in panelb, the ratio variation of fluorescence intensity and folic acid concentration (0.1-
50mg L-1) it is in a linear relationship, linearly dependent coefficient (r2) is 0.992, and the related coefficient of fitting of a polynomial then up to 0.999, is examined
Survey is limited to 9.8nM.As shown in Figure 4 C, the blue-fluorescence in FL-NIPs is gradually increased with folic acid concentration, but red fluorescence is strong
Degree variation is unobvious, and degree is quenched well below FL-MIPs, fluorescence color variation range is caused to be substantially reduced.Such as Fig. 4 D institute
Show, the fluorescence intensity in FL-NIPs ratio variation folic acid concentration between also present good linear fit with it is multinomial
Formula fitting, related coefficient reach 0.99 (r2) or more, so that calculating imprinting factor is 2.24.It should be the results show that FL-
There is the recognition site of specific binding folic acid in MIPs and does not have in FL-NIPs.
Application examples 5
The folic acid trace ratio fluorescent sensor (FL-MIPs) and non-trace ratio for taking 125 μ L above-described embodiments to obtain are glimmering
Light sensing (FL-NIPs) is separately added into 20mg L-1Folic acid, ninopterin (abbreviation MTX), methoxybenzyl aminopyrimidine (TMP), half
Cystine (Cys), histidine (His), arginine (Arg), glutamic acid (Glu) and vitamin C (Vc), it is then fixed with ultrapure water
Hold to 1mL and react 10min after mixing, then with the (excitation of the fluorescence spectrum of each sample of fluorescent spectrophotometer assay
Wavelength: 365nm, slit width 5/5nm).(referring to Fig. 5).
As shown in figure 5, the fluorescence intensity ratio variation of folic acid trace ratio fluorescent sensor (FL-MIPs) influences maximum,
This is because FL-MIPs contain largely and folic acid structure size, configuration, in terms of exact matching imprinted sites, from
And specific recognition target molecule folic acid, and other similar object then hardly enters imprinted cavity, can only carry out non-specific knot
It closes, so that quenching degree is lower, fluorescence intensity change is little.Meanwhile various analogs are to the ratio of the fluorescence intensity of FL-NIPs
Value variation influence is all little, this is because the surface FL-NIPs is there is no the recognition site of specificity, the identification between them is only
For non-specificity identification.
Application examples 6
Various concentration ratio is added in the folic acid trace ratio fluorescent sensor (FL-MIPs) for taking 125 μ L above-described embodiments to obtain
Ninopterin/folic acid (CMTX/CFA), it then is settled to 1mL with ultrapure water, after mixing, 10min is reacted, then with glimmering
The fluorescence spectrum of each sample of light spectrophotometric determination.Wherein the concentration of folic acid is fixed as 20mg L-1, ninopterin/folic acid
Concentration ratio be then followed successively by 0,1,2,3,4,5.(excitation wavelength: 365nm, slit width 5/5nm).(referring to illustration in Fig. 5)
As shown in illustration in Fig. 5, as ninopterin concentration is gradually increased, trace ratio fluorescent senses (FL-MIPs)
Fluorescence intensity ratio variation quite, the result prove trace ratio fluorescent sensing (FL-MIPs) to object folic acid have compared with
High selectivity.
Claims (9)
1. a kind of visualization molecular engram nano-sensor, characteristic are: visualization molecular engram nano-sensor passes through one
Footwork polymerize trace in Nano particles of silicon dioxide surface sol-gel, embeds red fluorescence cadmium telluride quantum dot (CdTe
QDs) and the folic acid with spontaneous blue-fluorescence, using the hole for eluting folic acid as recognition site, obtain that there are the double of core-shell structure
Fluorescent emission folic acid trace microballoon.
2. a kind of preparation method of visualization molecular engram nano-sensor described in claim 1, characteristic are: Xiang Hanyou
Red fluorescence cadmium telluride quantum dot (CdTe QDs), folic acid and 3- aminopropyl three are added in the solution of Nano particles of silicon dioxide
Ethoxysilane (APTES), stir 1-2h, continuously added after mixing ethyl orthosilicate (TEOS) and ammonium hydroxide under dark surrounds into
Row sol-gel polymerization reacts 10-12 hours, precipitates product with centrifugal process after reaction, abandons supernatant, elutes folic acid, obtains
Folic acid trace microballoon with core-shell structure, and be scattered in 6mL ultrapure water again.
3. the preparation method of visualization molecular engram nano-sensor as described in claim 2, characteristic are: described is molten
Glue-gel polymerisation reaction system total volume is controlled in 20-22mL；Wherein, Nano particles of silicon dioxide solution, CdTe QDs,
Folic acid, APTES, TEOS, ammonia volume are respectively 1-2mL, 3-5mL, 8-10mL, 37-75 μ L, 50-100 μ L, 50-100 μ L.
4. the preparation method of visualization molecular engram nano-sensor as described in claim 2, characteristic are: described two
Silicon oxide nanoparticle solution is that ammonium hydroxide is added in Xiang Shui/ethyl alcohol mixed solution, stirs 10-30min, after mixing with perseverance
The ethanol solution of ethyl orthosilicate is drop by drop added in pressure dropping funel, 10-12h is stirred to react, after the product centrifugation of acquisition
Liquid is discarded supernatant, ethanol washing precipitating is finally scattered in 50mL ultrapure water, for use again.
5. the preparation method of visualization molecular engram nano-sensor according to claim 4, characteristic are: the dioxy
The concentration of SiClx nanoparticle is 1.2-1.5mg/mL；The total volume of the water/alcohol mixed solution is 80-100mL, volume ratio
For 5:3；Volume in the TEOS ethanol solution is 25-30mL, and wherein the volume accounting of TEOS is 2%-3%.
6. the preparation method of visualization molecular engram nano-sensor as described in claim 2, characteristic are: the folic acid
Solution is that folic acid is dissolved in NaOH ethanol solution.
7. the preparation method of visualization molecular engram nano-sensor according to claim 6, characteristic are: the folic acid
Concentration be 1-1.2g/L；The concentration of the NaOH ethanol solution are as follows: 0.01-0.02molL-1, volume fraction shared by ethyl alcohol is
20-30%.
8. a kind of claim 1 wants the application of the visualization molecular engram nano-sensor, characteristic is: the sensing
The application of device nutrition fortifier folic acid in detection food and biological sample, serum.
9. the application of visualization molecular engram fluorescence nano sensor according to claim 8, characteristic are: the sensing
Device is in prepare liquid, and red fluorescence CdTe QDs in sensor signal in response, fluorescence intensity is with target concentration
Increase and reduce, and itself blue-fluorescence of object folic acid gradually increases, and passes through the change of difference emission peak intensity ratio at two
Change, to realize the visualization open hole detection to folic acid.
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| CN110736725A (en) * | 2019-09-30 | 2020-01-31 | 湘潭大学 | Preparation method and application of molecular imprinting fluorescent sensor for simultaneously and visually detecting two viruses |
| CN111024673A (en) * | 2020-01-20 | 2020-04-17 | 南京医科大学附属逸夫医院 | Ratiometric fluorescent molecularly imprinted polymer and preparation method and application thereof |
| CN112033943A (en) * | 2020-08-17 | 2020-12-04 | 中南民族大学 | Arginine detection method based on quantum dot-copper ion fluorescent substrate sensor |
| CN112485236A (en) * | 2020-11-26 | 2021-03-12 | 四川大学华西医院 | Homogeneous phase visualization and double-fluorescence signal analysis method based on multiple selective recognition reactions and application |
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