CN110169946A - Eye essence cream and preparation method thereof - Google Patents
Eye essence cream and preparation method thereof Download PDFInfo
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- CN110169946A CN110169946A CN201910463178.2A CN201910463178A CN110169946A CN 110169946 A CN110169946 A CN 110169946A CN 201910463178 A CN201910463178 A CN 201910463178A CN 110169946 A CN110169946 A CN 110169946A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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Abstract
The present invention provides a kind of eye essence cream, includes: on the basis of the total weight of eye essence cream, umbilical cord tissue extract 10%~40%, moisturizer 5%~20%, skin conditioning agent 5%~15%, emollient 1%~10%, emulsifier 1%~5%.The present invention also provides a kind of methods for preparing eye essence cream of the invention, include the following steps: to mix moisturizer, emollient, emulsifier, optionally add one or more of antioxidant, aromatic, chelating agent, thickener and deionized water, then 50~85 DEG C are heated the mixture to, it is stirred until homogeneous, wait be cooled to room temperature, umbilical cord tissue extract is added and skin conditioning agent is stirred until homogeneous, optionally adds after pH adjusting agent adjusts pH value to 5-7 and eye essence cream is made.Eye essence cream provided by the invention can be mild, and can tender skin whitening, smooth away wrinkles, pore refining.
Description
Technical field
The invention belongs to cosmetic fields.In particular it relates to a kind of eye essence cream and preparation method thereof.
Background technique
With the development of society, people are also increasingly to increase to the concern of skin.For women, with the increasing at age
Length is higher and higher to the attention rate of the eye skin of oneself.Eye skin is the part of most fragile in human skin.Reason first is that
Eye skin is almost without sweat gland and sebaceous glands, that is to say, that this part skin is hardly fuel-displaced, does not perspire.Due to lacking skin
The natural protection of adipose membrane, eye skin are easy drying, water shortage.Therefore, no matter you are any skin quality, and eye skin is all on the face
Desert.And the most easy problem of eye is just because of dry caused dry line and wrinkle.
Eyeware products and solution for lenses and contact lenses is dazzling currently on the market, how to select a kind of demand for being suitble to oneself again easy to use
Product is that consumer is concerned about.Occur the old eye product of many removing wrinkle and resisting agings in the market, but often excessively pursues effect, eye
Allergy rate after portion's skin use will greatly improve.
Summary of the invention
Therefore, the purpose of the present invention is to provide a kind of eye essence creams.Eye essence cream provided by the invention can be mild, and
And can tender skin whitening, smooth away wrinkles, pore refining.Meanwhile eye essence cream of the invention is prepared the present invention also provides a kind of
Method.
On the one hand, the present invention provides a kind of eye essence cream, includes: on the basis of the total weight of eye essence cream, umbilical cord
Tissue extract 10%~40%, moisturizer 5%~20%, skin conditioning agent 5%~15%, emollient 1%~10%, emulsification
Agent 1%~5%.
Preferably, in eye essence cream of the present invention, on the basis of the total weight of eye essence cream, the eye
Essence cream also includes pH adjusting agent 0.01%~0.2%, antioxidant 0.1~1%, aromatic 0.01%~0.5%, chelating agent
0.1%~1%, one or more of thickener 0.1%~1% and the deionized water of surplus.
Preferably, in eye essence cream of the present invention, the moisturizer is selected from glycerol, propylene glycol, methyl-prop two
Alcohol, 1,3- butanediol, 1,2- hexylene glycol, glycerin polyether -26, PEG/PPG-17/6 copolymer, PEG/PPG-14/7 dimethyl ether,
One or more of Sodium Hyaluronate, trehalose and glycine betaine；It is highly preferred that the moisturizer is selected from glycerol, 1,3- fourth two
Alcohol, 1,2- hexylene glycol, glycerin polyether -26, PEG/PPG-17/6 copolymer, Sodium Hyaluronate, trehalose, glycine betaine.
Preferably, in eye essence of the present invention cream, the skin conditioning agent be selected from oligopeptides -1, -1 copper of tripeptides,
Dipeptides diaminobutyryl benzyl amide diacetin, arginine/lysine polypeptide, Matrixyl -4, Argireline, palm fibre
Palmitic acid acyl tripeptides -1, carnosine, panthenol, biological carbohydrate gum -1, Sanguis Draxonis (Daemonorops draco) extract, glycosphingolipids, accumulated snow
Careless glycosides, Ba 2672, arginine, golden yellow chamomile (Chrysanthellum indicum) extract, purslane
(Portulaca oleracea) extract, Mi Luomu (Myrothamnus flabellifolia) leaf/stem extraction, millet
Green pepper (Capsicum frutescens) berry extract, grape fruit (Citrus paradisi) berry extract, butcher's broom (Ruscus
Aculeatus) root extract, meadow pine (Equisetum arvense) extract, glycyrrhiza glabra (Glycyrrhiza glabra)
Root extract, ascorbic acid methyl-monosilane alcohol pectate, methyl-monosilane alcohol hydroxyproline ester aspartate, dimethyl methyl silicon
Alkanol hyaluronic acid ester, aminoethyl phosphinic acids, tremella (Tremella fuciformis) fruiting body extract, Chinese herbaceous peony
(Paeonia albiflora) one or more of flower extract and hydrolyzed yeast extract；It is highly preferred that the skin tune
It manages agent and is selected from oligopeptides -1, Matrixyl -4, Argireline, carnosine, biological carbohydrate gum -1, kylin draconis extract, glycosphingolipid
It is class, asiaticosid, arginine, Tremella fructification extract, asiaticosid, golden yellow camomile extract, purslane extract, close
Luo Muye/stem extraction, millet starch berry extract, grape Fructus Citri grandis extract, butcher's broom root extract, Horsetail P.E, light fruit
Licorice root extract, ascorbic acid methyl-monosilane alcohol pectate, methyl-monosilane alcohol hydroxyproline ester aspartate, dimethyl
Silanol hyaluronic acid ester, aminoethyl phosphinic acids, Chinese herbaceous peony flower extract.The present invention is to the kylin draconis extract, golden yellow ocean
Flos Chrysanthemi extract, purslane extract, Mi Luomu leaf/stem extraction, millet starch berry extract, grape Fructus Citri grandis extract, phyllodium
Tree root extract, Horsetail P.E, glycyrrhiza glabra root extract, Tremella fructification extract, Chinese herbaceous peony flower extract and hydrolysis
Yeast extract is not particularly limited, commercial goods.
Preferably, in eye essence cream of the present invention, the emollient is selected from grape (Vitis vinifera)
Seed oil, Jojoba (Simmondsia chinensis) seed oil, oil tea (Camellia oleifera) seed oil, olive (Olea
Europaea) fruit oil, Babassu (Orbignya oleifera) seed oil, calophyllum inophyllum (Calophyllum inophyllum) seed
Oil, Butyrospermum parkii (Butyrospermum parkii) fruit rouge, cyclomethicone, dimethyl silicone polymer, isononanoic acid
One or more of different nonyl ester, caprylic/capric triglyceride and ethylhexyl palmitate；It is highly preferred that the emollient choosing
From olive oil, Butyrospermum parkii fruit rouge, cyclomethicone, dimethyl silicone polymer, isononyl isononanoate, octanoic acid/
Triglyceride DDD, ethylhexyl palmitate.
Preferably, in eye essence cream of the present invention, the emulsifier is selected from hydrolecithin, cetostearyl alcohol
In olive grease, Olivem 1000, cetostearyl alcohol, PEG-100 stearine, stearine and docosyl alcohol
One or more；It is highly preferred that the emulsifier is selected from hydrolecithin, cetostearyl alcohol olive grease, sorbierite olive
Grease, PEG-100 stearine, stearine.
Preferably, in eye essence cream of the present invention, the thickener is selected from carbomer, xanthan gum, ethoxy
One or more of cellulose, ammonium acryloyl taurate/VP copolymer and polyacrylate cross-linked polymer -6；More
Preferably, the thickener is carbomer, ammonium acryloyl taurate/VP copolymer.
Preferably, in eye essence cream of the present invention, the pH adjusting agent is selected from lactic acid, sodium citrate and citron
One or more of sour sodium；It is highly preferred that the PH regulator is lactic acid.
Preferably, in eye essence cream of the present invention, the antioxidant is selected from hydroxy decyl ubiquinone, the four hexyl last of the ten Heavenly stems
In alcohol acid ascorbyl ester, tocopherol acetate, pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamate) and Margarita extract
One or more；It is highly preferred that the antioxidant is tocopherol acetate and the (double-tert-butyl hydroxy hydrogen of pentaerythrite four
Change cinnamic acid) ester.Margarita extract is not particularly limited in the present invention, commercial goods.
Preferably, in eye essence cream of the present invention, the aromatic is selected from animal perfume material, plant perfume and people
One or more of work fragrance；It is highly preferred that the plant perfume is selected from rose oil, lavender oil, Pelargonium roseum leaf
One or more of oil and rosemary leaf oil.
Preferably, in eye essence cream of the present invention, the chelating agent is selected from EDETATE SODIUM, fragrant moss ketone, decoyl
One or more of hydroximic acid；It is highly preferred that the chelating agent is EDETATE SODIUM and fragrant moss ketone.
Preferably, in eye essence cream of the present invention, on the basis of the total weight of eye essence cream, each component
Weight percentage are as follows:
Umbilical cord tissue extract 30%, glycerol 4%, 1,3- butanediol 4%, glycerin polyether -26 4%, PEG/PPG-17/6
Copolymer 2%, Sodium Hyaluronate 0.1%, trehalose 2%, glycine betaine 2%, cyclomethicone 2%, poly dimethyl silicon
Oxygen alkane 1%, isononyl isononanoate 2%, caprylic/capric triglyceride 1%, ethylhexyl palmitate 2%, Butyrospermum parkii fruit rouge
1%, olive oil 1%, carbomer 0.2%, ammonium acryloyl taurate/VP copolymer 0.8%, oligopeptides -1
0.5%, Matrixyl -4 1%, Argireline 1%, carnosine 0.5%, biological carbohydrate gum -1 2%, kylin draconis extract
1%, glycosphingolipids 1%, asiaticosid 0.5%, arginine 0.5%, Tremella fructification extract 0.1%, asiaticosid
0.5%, golden yellow camomile extract 0.5%, purslane extract 0.5%, Mi Luomu leaf/stem extraction 0.5%, millet starch fruit
Extract 0.2%, grape Fructus Citri grandis extract 0.2%, butcher's broom root extract 0.2%, Horsetail P.E 0.2%, glycyrrhiza glabra
Root extract 0.2%, ascorbic acid methyl-monosilane alcohol pectate 0.2%, methyl-monosilane alcohol hydroxyproline ester aspartate
0.2%, dimethyl silane alcohol hyaluronic acid ester 0.2%, aminoethyl phosphinic acids 0.2%, Chinese herbaceous peony flower extract 1%, fragrant moss ketone
0.5%, tocopherol acetate 0.3%, 1,2- hexylene glycol 0.5%, oil of geranium 0.5%, (double-tertiary fourths of pentaerythrite four
Base hydroxyhydrocinnamic acid) ester 0.05%, EDETATE SODIUM 0.02%, hydrolecithin 1%, cetostearyl alcohol olive grease
0.5%, Olivem 1000 0.5%, cetostearyl alcohol 0.5%, PEG-100 stearine 0.5%, glycerol stearate
Ester 1%, docosyl alcohol 1%, lactic acid 0.01%, surplus deionized water.
Preferably, in eye essence cream of the present invention, the umbilical cord tissue extract is by including following step
Rapid method preparation:
(1) umbilical cord tissue is homogenized；
(2) the trypsase aqueous solution that concentration is 1-4g/L is added in the homogenised tissue that step (1) obtains, mixing is made
The mixed liquor is placed in anaerobic culture box in the case where not adding any culture medium and is incubated for by liquid；
(3) mixed liquor after the incubation for obtaining step (2) is centrifuged, and collects supernatant, while retaining the tissue substance of deposition；
(4) the trypsase aqueous solution that concentration is 20-30g/L is added in the tissue substance for the deposition that step (3) obtain, so
After stir, be centrifuged, collect supernatant；
(5) supernatant that step (3) and step (4) are collected is merged.
Preferably, the umbilical cord tissue is ox umbilical cord tissue.
Preferably, in step (1), umbilical cord tissue is cleaned with water before homogenate.It is highly preferred that the umbilical cord tissue after cleaning
It is impregnated with Iodophor, takes off iodine with alcohol after taking-up.
Preferably, the anaerobic culture box incubation in the step (2) is incubated for 24~72 hours at 35 DEG C~45 DEG C.
Preferably, the centrifugation in the step (3) and the step (4) be with 3000~10000r/min centrifugation 10~
20min。
Preferably, the method for preparing umbilical cord tissue extract is further comprising the steps of:
(6) microorganism and trypsase inactivation: the supernatant after the merging that step (5) are obtained inactivates, after collecting inactivation
Supernatant；Preferably, it is inactivated 10-30 minutes in 60 DEG C of -90 DEG C of water-baths；
(7) filtering of extract: the supernatant liquid filtering after the inactivation that step (6) are obtained is to remove pathogen；
(8) ultrafiltration of extract: positive/negative pressure is applied to the filtered fluid that step (7) obtain and carries out ultrafiltration, ultrafiltration is such as
Carry out under the conditions of lower: 0.3~1.0MPa of inlet pressure, outlet pressure -0.3~-1.0MPa, temperature are 2~8 DEG C；
(9) freeze-drying of extract saves: the filtrate that step (8) are obtained was chilled to -40 DEG C~-50 in 2~8 hours
DEG C, and be dried in vacuo.
Umbilical cord tissue extract stability made from the preparation method of the umbilical cord tissue extract provided through the invention is good,
Recovery rate height, pathogen-free domestic, it is former without heating, can save that (for example room temperature liquid and low temperature liquid save, often using a variety of methods
Temperature or cryo-conservation freeze-dried powder).
On the other hand, the present invention also provides a kind of method for preparing eye essence cream of the invention, include the following steps:
Moisturizer, emollient, emulsifier are mixed, and optionally add antioxidant, aromatic, chelating agent, thickener
And one or more of deionized water, 50~85 DEG C are then heated the mixture to, is stirred until homogeneous, room temperature to be down to
Afterwards, umbilical cord tissue extract is added and skin conditioning agent is stirred until homogeneous, optionally add pH adjusting agent and adjust pH value to 5-7
Eye essence cream is made afterwards.
Beneficial effects of the present invention:
Eye essence cream provided by the invention can be mild, and can tender skin whitening, smooth away wrinkles, pore refining.
Detailed description of the invention
Hereinafter, carrying out the embodiment that the present invention will be described in detail in conjunction with attached drawing, in which:
Fig. 1 show eye essence cream prepared by the embodiment of the present invention 1 tender skin, whitening, it is compact, smooth away wrinkles, shrink
Effect in terms of pore.
Fig. 2 is the accelerated test figure of the umbilical cord extract prepared using conventional method and the method for the present invention.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.
Embodiment 1
The preparation of umbilical cord tissue extract:
(1) processing of umbilical cord tissue: the in vitro umbilical cord tissue after healthy cow spontaneous labor after inspection and quarantine of learning from else's experience is used
It after silk thread tightens umbilical cord both ends, is soaked in physiological saline, transports laboratory back at 4 DEG C, clean umbilical cord group with sterilizing pure water
After knitting, after removing umbilical cord both ends with aseptic operation scissors, is taken out after immersion 1min in Iodophor and take off iodine with 75% alcohol, with sterilizing
Pure water rinsing 3 times, and aseptically mechanical homogenate.
(2) the sterilizing pure water and 3 times of bodies of 30 times of volumes the enrichment of extract: is added in the homogenised tissue that step (1) obtains
Long-pending concentration is the trypsase of 2.5g/L, mixed liquor is made, by the mixed liquor in the case where not adding any culture medium
It is placed in anaerobic culture box and is incubated for 35 hours at 40 DEG C, carry out hungry stimulation.
(3) clarified separation of extract: the mixed liquor after the incubation of step (2) acquisition is centrifuged with 6000r/min
15min collects 8 DEG C of supernatant preservations.Retain the tissue substance of deposition simultaneously.
(4) enzymatic hydrolysis of extract: the concentration that 3 times of volumes are added in the tissue substance for the deposition that step (3) obtain is 25g/L
Trypsase, be subsequently placed in after being stirred 36 hours in blender, be added equal volume sterilizing pure water mix after, with 6000r/
Min is centrifuged 15min, collects 8 DEG C of supernatant preservations.
(5) merge supernatant: the supernatant that step (3) and step (4) are collected is merged.
(6) microorganism and trypsase inactivation: the supernatant after step (5) are merged inactivates 30 points in 60 DEG C of water-baths
Clock, the supernatant after collecting inactivation.
(7) filtration sterilization of extract: by step (6) inactivation after supernatant, pass sequentially through aperture be 120 μm, 70 μm,
25 μm, the filter of 100nm, 50nm, 20nm remove pathogen, filter condition are as follows: inlet pressure 0.6MPa, temperature are 8 DEG C, are collected
Filtered fluid.
(8) the ultrafiltration molecular film of extract: the filtering liquid that step (7) are collected applies positive/negative pressure and crosses 50000 respectively
The filter membrane of dalton and 5000 Dalton molecular weights, being greater than 5000 dalton less than 50000 dalton is cell factor, is less than
5000 dalton are complex polypeptide, collect filtered fluid, filter condition respectively are as follows: inlet pressure 0.6MPa, outlet pressure-
0.6MPa, temperature are 8 DEG C, cryo-conservation.
(9) freeze-drying of extract saves: the filtered fluid packing that step (8) obtain is placed in cillin bottle, in 5 hours,
Be chilled to -45 DEG C, after vacuum drying, be temperature rise to room temperature can be stored at room temperature for a long time it is spare.
The preparation of eye essence cream:
On the basis of the total weight of eye essence cream, each component and its weight percentage (wt%) are as follows:
Component A: glycerol 4%, 1,3- butanediol 4%, glycerin polyether -26 4%, PEG/PPG-17/6 copolymer 2%, thoroughly
Bright matter acid sodium 0.1%, trehalose 2%, glycine betaine 2%, cyclomethicone 2%, dimethyl silicone polymer 1%, different nonyl
The different nonyl ester 2% of acid, caprylic/capric triglyceride 1%, ethylhexyl palmitate 2%, Butyrospermum parkii fruit rouge 1%, olive oil
1%, carbomer 0.2%, ammonium acryloyl taurate/VP copolymer 0.8%, fragrant moss ketone 0.5%, tocopherol acetate
0.3%, 1,2- hexylene glycol 0.5%, oil of geranium 0.5%, pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamate)
0.05%, EDETATE SODIUM 0.02%, hydrolecithin 1%, cetostearyl alcohol olive grease 0.5%, Olivem 1000
0.5%, cetostearyl alcohol 0.5%, PEG-100 stearine 0.5%, stearine 1%, docosyl alcohol 1%, surplus
Deionized water；
B component: umbilical cord tissue extract 30% manufactured in the present embodiment, oligopeptides -1 0.5%, Matrixyl -4 1%,
Argireline 1%, carnosine 0.5%, biological carbohydrate gum -1 2%, kylin draconis extract 1%, glycosphingolipids 1%, centella
Glycosides 0.5%, arginine 0.5%, Tremella fructification extract 0.1%, asiaticosid 0.5%, golden yellow camomile extract
0.5%, purslane extract 0.5%, Mi Luomu leaf/stem extraction 0.5%, millet starch berry extract 0.2%, grape fruit fruit mention
Take object 0.2%, butcher's broom root extract 0.2%, Horsetail P.E 0.2%, glycyrrhiza glabra root extract 0.2%, ascorbic acid
Methyl-monosilane alcohol pectate 0.2%, methyl-monosilane alcohol hydroxyproline ester aspartate 0.2%, dimethyl silane alcohol are saturating
Bright matter acid esters 0.2%, aminoethyl phosphinic acids 0.2%, Chinese herbaceous peony flower extract 1%；
Component C: lactic acid 0.01%；
After component A is mixed according to the above mentioned proportion, 60 DEG C are heated to, stirring 20min fusion mixes.Wait be cooled to room temperature,
B component is added to stir and evenly mix, add component C adjust pH value to after 5 product.
Embodiment 2
The preparation of umbilical cord tissue extract:
(1) processing of umbilical cord tissue: the in vitro umbilical cord tissue after healthy cow spontaneous labor after inspection and quarantine of learning from else's experience is used
It after silk thread tightens umbilical cord both ends, is soaked in physiological saline, transports laboratory back at 4 DEG C, clean umbilical cord group with sterilizing pure water
After knitting, after removing umbilical cord both ends with aseptic operation scissors, is taken out after immersion 3min in Iodophor and take off iodine with 60% alcohol, with sterilizing
Pure water rinsing 1 time, and aseptically mechanical homogenate.
(2) the sterilizing pure water and 1 times of volume of 1 times of volume the enrichment of extract: is added in the homogenised tissue that step (1) obtains
Concentration be 1g/L trypsase, mixed liquor is made, the mixed liquor is placed in the case where not adding any culture medium
It is incubated for 24 hours at 45 DEG C in anaerobic culture box, carries out hungry stimulation.
(3) clarified separation of extract: the mixed liquor after the incubation of step (2) acquisition is centrifuged with 10000r/min
10min collects 2 DEG C of supernatant preservations.Retain the tissue substance of deposition simultaneously.
(4) enzymatic hydrolysis of extract: the concentration that 1 times of volume is added in the tissue substance for the deposition that step (3) obtain is 30g/L
Trypsase, be subsequently placed in after being stirred 24 hours in blender, be added equal volume sterilizing pure water mix after, with 3000r/
Min is centrifuged 20min, collects 6 DEG C of supernatant preservations.
(5) merge supernatant: the supernatant that step (3) and step (4) are collected is merged.
(6) microorganism and trypsase inactivation: the supernatant after step (5) are merged inactivates 25 points in 60 DEG C of water-baths
Clock, the supernatant after collecting inactivation.
(7) filtration sterilization of extract: by step (6) inactivation after supernatant, pass sequentially through aperture be 120 μm, 70 μm,
25 μm, the filter of 100nm, 50nm, 20nm remove pathogen, filter condition are as follows: inlet pressure 0.3MPa, temperature are 4 DEG C, are collected
Filtered fluid.
(8) the ultrafiltration molecular film of extract: the filtering liquid that step (7) are collected applies positive/negative pressure and crosses 50000 respectively
The filter membrane of dalton and 5000 Dalton molecular weights, being greater than 5000 dalton less than 50000 dalton is cell factor, is less than
5000 dalton are complex polypeptide, collect filtered fluid, filter condition respectively are as follows: inlet pressure 0.3MPa, outlet pressure-
0.3MPa, temperature are 6 DEG C, cryo-conservation.
(9) freeze-drying of extract saves: the filtered fluid packing that step (8) obtain is placed in cillin bottle, in 5 hours,
Be chilled to -44 DEG C, after vacuum drying, be temperature rise to room temperature can be stored at room temperature for a long time it is spare.
The preparation of eye essence cream:
On the basis of the total weight of eye essence cream, each component and its weight percentage (wt%) are as follows:
Component A: glycerol 1%, 1,3- butanediol 1%, PEG/PPG-17/6 copolymer 2%, Sodium Hyaluronate 0.1%, sea
Algae sugar 2%, glycine betaine 1%, grape seed oil 0.1%, dimethyl silicone polymer 0.5%, isononyl isononanoate 0.3%, the octanoic acid/last of the ten Heavenly stems
Acid glycerol three ester 1%, ethylhexyl palmitate 2%, Butyrospermum parkii fruit rouge 1%, olive oil 0.1%, xanthan gum 0.05%,
Hydroxyethyl cellulose 0.05%, decoyl hydroximic acid 0.5%, hydroxy decyl ubiquinone 0.3%, 1,2- hexylene glycol 0.5%, Pelargonium roseum
Oily 0.5%, pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamate) 0.7%, EDETATE SODIUM 0.5%, cetostearyl alcohol olive
Olive grease 0.5%, Olivem 1000 0.5%, surplus deionized water；
B component: umbilical cord tissue extract 10% manufactured in the present embodiment, oligopeptides -1 0.5%, -1 copper 0.5% of tripeptides, second
Acyl group hexapeptide -8 0.5%, carnosine 0.5%, biological carbohydrate gum -1 1%, kylin draconis extract 0.1%, glycosphingolipids 0.1%, product
Asiaticoside 0.5%, arginine 0.5%, Tremella fructification extract 0.1%, asiaticosid 0.5%, golden yellow camomile extract
0.5%, panthenol 0.1%, methyl-monosilane alcohol hydroxyproline ester aspartate 0.2%, dimethyl silane alcohol hyaluronic acid ester
0.2%, aminoethyl phosphinic acids 0.2%, Chinese herbaceous peony flower extract 1%；
Component C: sodium citrate 0.2%；
After component A is mixed according to the above mentioned proportion, 50 DEG C are heated to, stirring 30min fusion mixes.Wait be cooled to room temperature,
B component is added to stir and evenly mix, add component C adjust pH value to after 7 product.
Embodiment 3
The preparation of umbilical cord tissue extract:
(1) processing of umbilical cord tissue: the in vitro umbilical cord tissue after healthy cow spontaneous labor after inspection and quarantine of learning from else's experience is used
It after silk thread tightens umbilical cord both ends, is soaked in physiological saline, transports laboratory back at 4 DEG C, clean umbilical cord group with sterilizing pure water
After knitting, after removing umbilical cord both ends with aseptic operation scissors, is taken out after immersion 5min in Iodophor and take off iodine with 90% alcohol, with sterilizing
Pure water rinsing 5 times, and aseptically mechanical homogenate.
(2) the sterilizing pure water and 5 times of bodies of 50 times of volumes the enrichment of extract: is added in the homogenised tissue that step (1) obtains
Long-pending concentration is the trypsase of 4g/L, and mixed liquor is made, and the mixed liquor is underlying the case where not adding any culture medium
It is incubated for 72 hours at 35 DEG C in anaerobic culture box, carries out hungry stimulation.
(3) clarified separation of extract: the mixed liquor after the incubation of step (2) acquisition is centrifuged with 3000r/min
20min collects 8 DEG C of supernatant preservations.Retain the tissue substance of deposition simultaneously.
(4) enzymatic hydrolysis of extract: the concentration that 5 times of volumes are added in the tissue substance for the deposition that step (3) obtain is 20g/L
Trypsase, be subsequently placed in after being stirred 72 hours in blender, be added equal volume sterilizing pure water mix after, with
10000r/min is centrifuged 10min, collects 2 DEG C of supernatant preservations.
(5) merge supernatant: the supernatant that step (3) and step (4) are collected is merged.
(6) microorganism and trypsase inactivation: the supernatant after step (5) are merged inactivates 10 points in 90 DEG C of water-baths
Clock, the supernatant after collecting inactivation.
(7) filtration sterilization of extract: by step (6) inactivation after supernatant, pass sequentially through aperture be 120 μm, 70 μm,
25 μm, the filter of 100nm, 50nm, 20nm remove pathogen, filter condition are as follows: inlet pressure 1.0MPa, temperature are 2 DEG C, are collected
Filtered fluid.
(8) the ultrafiltration molecular film of extract: the filtering liquid that step (7) are collected applies positive/negative pressure and crosses 50000 respectively
The filter membrane of dalton and 5000 Dalton molecular weights, being greater than 5000 dalton less than 50000 dalton is cell factor, is less than
5000 dalton are complex polypeptide, collect filtered fluid, filter condition respectively are as follows: inlet pressure 1.0MPa, outlet pressure-
1.0MPa, temperature are 2 DEG C, cryo-conservation.
(9) freeze-drying of extract saves: the filtered fluid packing that step (8) obtain is placed in cillin bottle, in 8 hours,
Be chilled to -40 DEG C, after vacuum drying, be temperature rise to room temperature can be stored at room temperature for a long time it is spare.
The preparation of eye essence cream:
On the basis of the total weight of eye essence cream, each component and its weight percentage (wt%) are as follows:
Component A: glycerol 1%, 1,3- butanediol 1%, PEG/PPG-17/6 copolymer 2%, Sodium Hyaluronate 2%, seaweed
Sugared 2%, glycine betaine 1%, grape seed oil 0.1%, dimethyl silicone polymer 0.5%, isononyl isononanoate 0.3%, caprylic/capric
Triglycerides 1%, ethylhexyl palmitate 2%, Butyrospermum parkii fruit rouge 1%, olive oil 0.1%, xanthan gum 0.05%, hydroxyl
Ethyl cellulose 0.05%, decoyl hydroximic acid 0.05%, tocopherol acetate 0.05%, 1,2- hexylene glycol 0.5%, Pelargonium
Certain herbaceous plants with big flowers oil 0.5%, four hexyl decyl alcohol acid ascorbyl esters 0.5%, EDETATE SODIUM 0.05%, cetostearyl alcohol olive grease 0.5%, mountain
The deionized water of pears alcohol olive grease 0.5%, surplus；
B component: umbilical cord tissue extract 40% manufactured in the present embodiment, oligopeptides -1 0.5%, -1 copper 0.5% of tripeptides, second
Acyl group hexapeptide -8 0.5%, carnosine 0.5%, biological carbohydrate gum -1 1%, kylin draconis extract 0.5%, golden yellow camomile extract
1%, panthenol 0.5%, methyl-monosilane alcohol hydroxyproline ester aspartate 0.2%, dimethyl silane alcohol hyaluronic acid ester
0.2%, aminoethyl phosphinic acids 0.2%, Chinese herbaceous peony flower extract 1%；
Component C: sodium citrate 0.1%；
After component A is mixed according to the above mentioned proportion, 85 DEG C are heated to, stirring 15min fusion mixes.Wait be cooled to room temperature,
B component is added to stir and evenly mix, add component C adjust pH value to after 6 product.
Embodiment 4
Umbilical cord extract accelerated test:
According to bioproduct stability investigative technique guideline, accelerates and mandatory condition test can be used for understanding product
The steadiness of product in the case where deviateing preservation condition and extreme case in short term.
The umbilical cord extract of umbilical cord extract and conventional method preparation prepared by embodiment 1 carries out accelerated test.Wherein,
The method that conventional method prepares umbilical cord extract is as follows:
(1) processing of umbilical cord tissue: the in vitro umbilical cord tissue after healthy cow spontaneous labor after inspection and quarantine of learning from else's experience is used
It after silk thread tightens umbilical cord both ends, is soaked in physiological saline, transports laboratory back at 4 DEG C, clean umbilical cord group with sterilizing pure water
After knitting, after removing umbilical cord both ends with aseptic operation scissors, is taken out after immersion 1min in Iodophor and take off iodine with 75% alcohol, with sterilizing
Pure water rinsing 3 times, and aseptically mechanical homogenate.
(2) tryptose of the 10g/L of equal volume the enzymatic hydrolysis of extract: is added in the homogenised tissue object that step (1) obtains
Enzyme after 6 hours of room temperature, is centrifuged 20min with 4000r/min, collects 2 DEG C of supernatant preservations.
(3) supernatant that step (2) are collected microorganism and trypsase inactivation: is inactivated 30 points in 60 DEG C of water-baths
Clock, the supernatant after collecting inactivation.
(4) filtration sterilization of extract: by the liquid after step (3) inactivation, passing sequentially through aperture is 120 μm, 70 μm, 25
μm, the filter of 100nm, 50nm, 20nm remove pathogen, filter condition is into 0.3MPa, and temperature is 8 DEG C, collects filtered fluid.
(5) ultrafiltration of extract: by step (4) collect filtered fluid, apply positive/negative pressure respectively cross 50000 dalton and
The filter membrane of 5000 Dalton molecular weights, being greater than 5000 dalton less than 50000 dalton is cell factor, less than 5000 dalton
For complex polypeptide, filtered fluid, filter condition are collected are as follows: into 0.3MPa, -0.3MPa out, the umbilical cord extract of acquisition.
Experimental condition is 40 DEG C, relative humidity 75%, and multiple multigelation is carried out in 15 days test periods.Figure
2 be the accelerated test figure of the umbilical cord extract prepared using conventional method and the method for the present invention.The a bottles of navels for conventional method preparation
With after extract progress accelerated test as a result, the umbilical cord extract that b bottles are the preparation of embodiment 1 carries out the knot after accelerated tests
Fruit.In Fig. 2, though a bottles of sample clarifications, have a large amount of floccules to generate, and b bottles of samples are clarified and are generated without floccule.Thus may be used
Know, the extract stability prepared using the method for the present invention is higher.
Embodiment 5
Using effect experiment
Using the cream of eye essence obtained by embodiment 1, to problem eye part skin, such as tender skin, whitening, compact, contraction
The using effect of pore is shown in Fig. 1.
Fig. 1 show eye essence cream prepared by the embodiment of the present invention 1 tender skin, whitening, it is compact, smooth away wrinkles, shrink
Effect in terms of pore；Before using eye essence cream of the invention: pore is coarse, has wrinkle, skin relaxation；Using this
The eye essence cream one of invention is after Zhou Zhisan weeks: skin quality, which tenderizes, to bleach, is compact, and pore is shunk.As it can be seen that prepared by the present invention
Eye essence cream tender skin, whitening, it is compact, smooth away wrinkles, in terms of pore refining have good effect.
Eye essence cream prepared by the embodiment of the present invention 2 and 3 is to eye part skin, such as tender skin, whitening, compact, contraction
Pore etc. also has the using effect such as embodiment 1.
Claims (10)
1. a kind of eye essence cream, includes: on the basis of the total weight of eye essence cream, umbilical cord tissue extract 10%~
40%, moisturizer 5%~20%, skin conditioning agent 5%~15%, emollient 1%~10%, emulsifier 1%~5%.
2. eye essence cream according to claim 1, wherein on the basis of the total weight of eye essence cream, the eye
Essence cream also includes pH adjusting agent 0.01%~0.2%, antioxidant 0.1~1%, aromatic 0.01%~0.5%, chelating agent
0.1%~1%, one or more of thickener 0.1%~1% and the deionized water of surplus.
3. eye essence cream according to claim 1, wherein the moisturizer is selected from glycerol, propylene glycol, methyl-prop two
Alcohol, 1,3- butanediol, 1,2- hexylene glycol, glycerin polyether -26, PEG/PPG-17/6 copolymer, PEG/PPG-14/7 dimethyl ether,
One or more of Sodium Hyaluronate, trehalose and glycine betaine.
4. eye essence cream according to claim 1, wherein the skin conditioning agent is selected from oligopeptides -1, -1 copper of tripeptides, two
Peptide diaminobutyryl benzyl amide diacetin, arginine/lysine polypeptide, Matrixyl -4, Argireline, palm
Acyl tripeptides -1, carnosine, panthenol, biological carbohydrate gum -1, kylin draconis extract, glycosphingolipids, asiaticosid, Ba 2672, smart ammonia
Sour, golden yellow camomile extract, purslane extract, Mi Luomu leaf/stem extraction, millet starch berry extract, grape fruit fruit mention
Take object, butcher's broom root extract, Horsetail P.E, glycyrrhiza glabra root extract, ascorbic acid methyl-monosilane alcohol pectate, first
Base silane alcohol hydroxyproline ester aspartate, dimethyl silane alcohol hyaluronic acid ester, aminoethyl phosphinic acids, Tremella fructification
One or more of extract, Chinese herbaceous peony flower extract and hydrolyzed yeast extract.
5. eye essence cream according to claim 1, wherein the emollient is selected from grape seed oil, jojoba seed oil, oil
Tea-seed oil, olive oil, Babassu seed oil, Malus spectabilis fruit seed oil, Butyrospermum parkii fruit rouge, cyclomethicone, poly- diformazan
One or more of radical siloxane, isononyl isononanoate, caprylic/capric triglyceride and ethylhexyl palmitate.
6. eye essence cream according to claim 1, wherein the emulsifier is selected from hydrolecithin, cetostearyl alcohol
In olive grease, Olivem 1000, cetostearyl alcohol, PEG-100 stearine, stearine and docosyl alcohol
One or more.
7. eye essence cream according to claim 2, wherein it is fine that the thickener is selected from carbomer, xanthan gum, ethoxy
One or more of dimension element, ammonium acryloyl taurate/VP copolymer and polyacrylate cross-linked polymer -6；
Preferably, the pH adjusting agent is selected from one or more of lactic acid, sodium citrate and sodium citrate；
Preferably, the antioxidant is selected from hydroxy decyl ubiquinone, four hexyl decyl alcohol acid ascorbyl esters, tocopherol acetate, season penta
Tetrol four (double-tert-butyl hydroxy hydrocinnamic acid) one or more of ester and Margarita extract；
Preferably, the aromatic is selected from one or more of animal perfume material, plant perfume and artificial flavors；It is highly preferred that
The plant perfume is selected from one or more of rose oil, lavender oil, Pelargonium roseum leaf oil and rosemary leaf oil；
Preferably, the chelating agent is selected from one or more of EDETATE SODIUM, fragrant moss ketone, decoyl hydroximic acid.
8. eye essence cream according to claim 2, wherein on the basis of the total weight of eye essence cream, each component
Weight percentage are as follows:
Umbilical cord tissue extract 30%, glycerol 4%, 1,3- butanediol 4%, glycerin polyether -26 4%, PEG/PPG-17/6 copolymerization
Object 2%, Sodium Hyaluronate 0.1%, trehalose 2%, glycine betaine 2%, cyclomethicone 2%, dimethyl silicone polymer
1%, isononyl isononanoate 2%, caprylic/capric triglyceride 1%, ethylhexyl palmitate 2%, Butyrospermum parkii fruit rouge 1%, oil
Olive fruit oil 1%, carbomer 0.2%, ammonium acryloyl taurate/VP copolymer 0.8%, oligopeptides -1 0.5%, palm
Acyl pentapeptide -4 1%, Argireline 1%, carnosine 0.5%, biological carbohydrate gum -1 2%, kylin draconis extract 1%, glycosphingolipid
Class 1%, asiaticosid 0.5%, arginine 0.5%, Tremella fructification extract 0.1%, asiaticosid 0.5%, golden yellow ocean are sweet
Chrysanthemum extract 0.5%, purslane extract 0.5%, Mi Luomu leaf/stem extraction 0.5%, millet starch berry extract 0.2%, Portugal
Grape Fructus Citri grandis extract 0.2%, butcher's broom root extract 0.2%, Horsetail P.E 0.2%, glycyrrhiza glabra root extract 0.2%,
Ascorbic acid methyl-monosilane alcohol pectate 0.2%, methyl-monosilane alcohol hydroxyproline ester aspartate 0.2%, dimethyl methyl
Silanol hyaluronic acid ester 0.2%, aminoethyl phosphinic acids 0.2%, Chinese herbaceous peony flower extract 1%, fragrant moss ketone 0.5%, tocopherol second
Acid esters 0.3%, 1,2- hexylene glycol 0.5%, oil of geranium 0.5%, the (double-tert-butyl hydroxy hydrocinnamoyl of pentaerythrite four
Acid) ester 0.05%, EDETATE SODIUM 0.02%, hydrolecithin 1%, cetostearyl alcohol olive grease 0.5%, sorbierite olive oil
Ester 0.5%, cetostearyl alcohol 0.5%, PEG-100 stearine 0.5%, stearine 1%, docosyl alcohol 1%, cream
The deionized water of acid 0.01%, surplus.
9. eye essence cream according to claim 1, wherein the umbilical cord tissue extract is by including the following steps
Method preparation:
(1) umbilical cord tissue is homogenized；
(2) the trypsase aqueous solution that concentration is 1-4g/L is added in the homogenised tissue that step (1) obtains, mixed liquor is made,
The mixed liquor is placed in anaerobic culture box in the case where not adding any culture medium to be incubated for；
(3) mixed liquor after the incubation for obtaining step (2) is centrifuged, and collects supernatant, while retaining the tissue substance of deposition；
(4) the trypsase aqueous solution that concentration is 20-30g/L is added in the tissue substance for the deposition that step (3) obtain, then stirs
It mixes, is centrifuged, collect supernatant；
(5) supernatant that step (3) and step (4) are collected is merged.
10. a kind of method for preparing eye essence cream of any of claims 1-9, includes the following steps:
By moisturizer, emollient, emulsifier mix, and optionally add antioxidant, aromatic, chelating agent, thickener and
Then one or more of deionized water heats the mixture to 50~85 DEG C, is stirred until homogeneous, wait be cooled to room temperature, add
Enter umbilical cord tissue extract and skin conditioning agent is stirred until homogeneous, optionally adds after pH adjusting agent adjusts pH value to 5-7 and make
Obtain eye essence cream.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112206200A (en) * | 2020-09-18 | 2021-01-12 | 珠海海狮龙生物科技有限公司 | Full-effect crystal eye mask and preparation method thereof |
| CN112274629A (en) * | 2020-11-18 | 2021-01-29 | 彭祥丽 | Dampness-eliminating cold-dispelling physique-dredging health-care oil composition and preparation method thereof |
| CN112402354A (en) * | 2020-10-30 | 2021-02-26 | 广州市妆妍生物技术有限公司 | Moisturizing and moisture-preserving emulsion and preparation method thereof |
| CN112716814A (en) * | 2020-12-29 | 2021-04-30 | 彭氏(惠州)实业发展有限公司 | Double-layer shaking essence and preparation method thereof |
| CN114652665A (en) * | 2020-12-23 | 2022-06-24 | 天津拂瑞雅生物科技有限公司 | Skin care essence and preparation method thereof |
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| CN115569099A (en) * | 2022-10-24 | 2023-01-06 | 浙江海心智惠科技有限公司 | Essence and preparation method thereof, and repairing patch and preparation method thereof |
| CN116077358A (en) * | 2021-11-05 | 2023-05-09 | 长春圣博玛生物材料有限公司 | Nutrient solution and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130007751A (en) * | 2011-07-11 | 2013-01-21 | (주)차바이오앤디오스텍 | Methods for preparing extract of umbilical cord and uses thereof |
| CN103998048A (en) * | 2011-07-11 | 2014-08-20 | 车比奥及戴奥斯泰有限公司 | Method for manufacturing umbilical cord extract and usage of same |
| CN106580798A (en) * | 2016-12-30 | 2017-04-26 | 广州科盈化妆品有限公司 | All-in-one eye cream and preparation method |
-
2019
- 2019-05-30 CN CN201910463178.2A patent/CN110169946B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130007751A (en) * | 2011-07-11 | 2013-01-21 | (주)차바이오앤디오스텍 | Methods for preparing extract of umbilical cord and uses thereof |
| CN103998048A (en) * | 2011-07-11 | 2014-08-20 | 车比奥及戴奥斯泰有限公司 | Method for manufacturing umbilical cord extract and usage of same |
| CN106580798A (en) * | 2016-12-30 | 2017-04-26 | 广州科盈化妆品有限公司 | All-in-one eye cream and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 江苏物恒生物科技有限公司: "极妍家 多效赋活修护眼膜", 《HTTP://FTBA.NMPA.GOV.CN:8181/FTBAN/ITOWNET/HZP_BA/FW/PZ.JSP?PROCESSID=20171220155137E82U1&NID=20171220155137E82U1》 * |
Cited By (13)
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|---|---|---|---|---|
| CN112206200A (en) * | 2020-09-18 | 2021-01-12 | 珠海海狮龙生物科技有限公司 | Full-effect crystal eye mask and preparation method thereof |
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| CN114652665A (en) * | 2020-12-23 | 2022-06-24 | 天津拂瑞雅生物科技有限公司 | Skin care essence and preparation method thereof |
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| CN112716814A (en) * | 2020-12-29 | 2021-04-30 | 彭氏(惠州)实业发展有限公司 | Double-layer shaking essence and preparation method thereof |
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| CN116077358A (en) * | 2021-11-05 | 2023-05-09 | 长春圣博玛生物材料有限公司 | Nutrient solution and preparation method and application thereof |
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