CN110157638A - A kind of gram lining institute series bacillus YY-1 and its application - Google Patents
A kind of gram lining institute series bacillus YY-1 and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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Abstract
The invention discloses a kind of gram lining institute's series bacillus and its application, bacterial strain for a gram lining institute series bacillus (Paenibacillus kribbensis) YY-1, be preserved in China typical culture collection center, depositary institution address: the Chinese Wuhan Wuhan University, the deposit date is on April 8th, 2019, deposit number was CCTCC NO.M2019242.The bacterial strain has significant inhibiting effect to the disease fungus black rot of pear bacterium of trunks of fruit trees, can effectively preventing black rot of pear, and the bacterial strain is harmless to ecological environment, will not cause the drug resistance of cause of disease, and condition of culture is simple, is easy to save;Biocontrol agent preparation method provided by the invention is simple, easy to industrialized production, with good development and application prospects.
Description
Technical field
The invention belongs to technical field of biological control, in particular to one kind gram lining institute series bacillus YY-1 and its answer
With.
Background technique
Black rot of pear, also known as scurf, it is widely distributed in each pears producing region in China, it is one of important disease of China pear tree.
Disease main harm pear tree limb, fruit and the blade, limb is aggrieved to promote branch early ageing, and blade is aggrieved to cause blade early
It falls, fruit is aggrieved to cause a large amount of decayed fruits, seriously affect yield and quality.By black rot of pear bacterium (Botryosphaeria dothidea) caused by ring spot be that pears producing region occurs and one of the serious disease that causes harm throughout the year, especially with rotten caused by storage phase
Fruit loss is most heavy, and while infecting pear tree limb is difficult to clean off, and provides germ source to infect again.China is every year because of pears wheel
Line disease causes the operatic circle underproduction about 25%, and decayed fruit rate is more than 80% when serious.The germ not only infects pear tree, apple, grape, peach,
It is also harmful on the fruit trees such as Kiwi berry, blueberry.
Black rot of pear bacterium be Ascomycota grape seat chamber Cordycepps (Botryosphaeriaceae) Botryosphaeria
(Botryosphaeria) type sepecies, can be saprophytic or parasitize in plant, or within raw fungal morphology exist.The bacterium is alive
Boundary various regions are widely distributed, can infect a variety of fruit trees and forest, cause citrus fruit rot, kiwi fruit maize ear rot, ring rot of apple,
Black rot of pear, pears dry rot, Kiwi berry branch-rot, grape limb canker, blueberry branch-rot, peach bleeding disease, cherry canker,
The multiple diseases such as Walnut Canker, Dothiorella Gregaria Sacc, dothiorella gregaria and Canker.Grape seat chamber bacterium mainly passes through bud, hole skin, stomata
Host's vascular tissue is infected with wound etc., initial stage causes host's limb ulcer or young sprout withered, and later period scab causes in flakes
Whole strain is dead, causes destructive consequence to ecological forest and economic fruit production.
Prevention and treatment for black rot of pear, relies primarily on cultivation management measure at present and chemical pesticide is prevented and treated.But due to
Black rot of pear has the characteristics that longer incubation period, longer disease cycle and multiple spot endanger more, and a life is needed in prevention and treatment
Long period repeatedly handles, multiple applications chemical pesticide, could preferably control its harm.There are control cost height, time-consuming, dirty
The problems such as contaminating environment.Therefore, seek new economical and efficient, environmentally protective Prevention Technique and approach and have become current planting fruit trees
Industry need to be solved the problems, such as urgently.
Bacillus genus (Paenibacillus) bacterium be a kind of amphimicrobian, endospore formed bacterial species, should
Class bacterium initially belong to bacillus (Bacillus), it is then re-classified as one and individually belongs to.Bacillus genus
Bacterial strain it is widely distributed, have detection from a variety of environment such as soil, water, rhizosphere, vegetables, feed and insect larvae and material.
Paenibacilli growth is fast, nutrition is simple, is capable of forming the gemma with stronger anti-adversity ability, and living bacteria count compared with
Non- bacillus is high, performance is stablized, therefore is widely used in product development.This kind of bacterium can generate various cells
Exoenzyme, such as polysaccharide degrading enzyme and protease, the various synthetic reactions of catalysis biological field of fuel production, also can produce antibacterial
Metabolite, inhibits the growth of pathogen, and induces plant generation system resistance.In addition, some series bacillus are as plant
Rhizosphere growth-promoting endophytic bacteria (plant growth-promoting rhizobacteria, PGPR), can competitively be colonized and plant
Object root system is not only used as bio-feritlizer, but also can play inhibiting effect to root system of plant pathogenetic bacteria, fungi and nematode.In addition, gram in
Cloth institute series bacillus (Paenibacillus kribbensis) also have the characteristics that antagonistic phytopathogen and potassium decomposing, phosphorus decomposing
With a variety of effects such as fixed nitrogen, crop yield can be improved in agricultural production, improve quality of agricultural product.Therefore gram lining institute class bud
Spore bacillus is agriculturally having a good application prospect.
Currently, using gram lining institute series bacillus controlling plant diseases at home and abroad it has been reported that how bright equal from Thailand
Gram lining institute series bacillus 4-531 being separated in mountain soil earth to cigarette brown spot pathogen (Alternaria alternate), it is yellow
Cucurbit wilt bacterium (Fusarium oxysporum) and rhizoctonia rot of cotton bacterium (Rhizoctonia solani) show it is stronger
Bacteriostatic activity;The phosphoric acid ester substance that gram lining institute series bacillus POC115 is generated to hungmao mentagrophyte (Trichophyton rubrum) there is antibacterial activity (identification of 1 plant of Antagonistic Bacteria Inhibiting Against Plant Pathogenic Fungi, Journal of Northwest Sci Tech University of Agriculture and Forestry, 2007);
The efficient phosphate-solubilizing potassium decomposing gram lining institute series bacillus strain CX-7 that Wang Wen etc. is screened from soil to verticillium dahliae,
Cotton-wilt fusarium etc. has bacteriostatic activity (screening of antagonism gram lining institute series bacillus CX-7 bacterial strain and point of antibacterial protein
From Agricultural University Of Hebei, 2012);Gram lining institute series bacillus T-9 separated from soil can seriously inhibit botrytis cinerea
(Botrytis cinerea), anthrax-bacilus (Colletotricum acutatum), Fusarium oxysporum (Fusarium oxysporum), Pyricularia oryzae (Magnaporthe oryzae), phytophthora blight of pepper (Phytophthora capsici), stand it is withered
Rhizoctonia (Rhizoctonia solani) and white rot pyrenomycetes (Sclerotium cepivorum) bacterium colony growth, and can prevent
Harm caused by these diseases are controlled on host plant;Gram lining institute series bacillus PS04 that Liao Meide etc. is separated from soil
To Rhizoctonia solani Kuhn (Rhizoctonia solani) there is stronger bacteriostatic activity, which, which mainly passes through, inhibits pathogen
Growth and induction host plant generate resistance play the role of diseases prevention (antifungal activity of PS04 bacterial strain, Changjiang University's journal,
2008).It can be seen that gram lining institute's series bacillus is because strain difference controlling plant diseases are also different, without foresight with
And corresponding enlightenment.And also about gram lining institute's series bacillus not there is the related of prevention and treatment fruit tree ring spot to report at present
Road.
Summary of the invention.
In view of the defects existing in the prior art, the purpose of the present invention is to provide one kind gram lining institute series bacillus YY-
1, which can prevent and treat trunks of fruit trees disease fungus black rot of pear bacterium, inhibit growth, breeding and the field planting of black rot of pear bacterium, rich
Rich biocontrol microorganisms libraries, provide more choices for the genetic modification of plant.
To achieve the above object, the present invention adopts the following technical scheme: the present invention provides a kind of gram lining institute class gemma
Bacillus strain, the bacterial strain for a gram lining institute series bacillus bacterial strain (Paenibacillus kribbensis) YY-1, it is preserved in
China typical culture collection center, depositary institution address: the Chinese Wuhan Wuhan University, the deposit date is April 8 in 2019
Day, deposit number is CCTCC NO.M2019242.
The present invention also provides above-mentioned gram of lining institute series bacillus YY-1 answering in prevention and treatment trunks of fruit trees disease fungus
With.
Further, the disease fungus is black rot of pear bacterium.
A kind of biocontrol agent, effective component are above-mentioned gram of lining institute series bacillus YY-1.
Further, described gram of lining institute series bacillus YY-1 is the culture bacteria suspension, fermentation liquid or fermentation point of the bacterial strain
From supernatant.
Further, the viable count of the fermentation liquid and gram lining institute series bacillus YY-1 in culture bacteria suspension is not less than 1
×105cfu/mL。
Further, the separation of fermentative broth supernatant, which is adopted, is prepared by the following steps: by gram lining institute series bacillus YY-1
It is resuspended to obtain bacteria suspension with sterile PD fluid nutrient medium after activation, then the bacteria suspension is inoculated in PD liquid with 5% inoculum concentration
In culture medium, shaken cultivation 48h~72h at 120~200r/min, 25~30 DEG C, then by obtained fermentation liquid in 4 DEG C
6000 ~ 8000rpm centrifuging and taking supernatant is finally filtered supernatant up on the separation of fermentative broth using 0.22 nm sterilised membrane filter
Clear liquid.
The present invention also provides above-mentioned biocontrol agents in prevention and treatment ring rot of apple, black rot of pear, pears dry rot, macaque peach branch
Application in blight, grape limb canker or blueberry branch-rot.
Compared with prior art, the invention has the following beneficial effects:
1, present invention screening on the cockscomb blade of health obtains a gram lining institute series bacillus YY-1, which passes through generation
Antibacterial substance come inhibit black rot of pear bacterium (Botryosphaeria dothidea) growth and infect, therefore gram lining institute class
Bacillus YY-1 can not only directly inhibit the growth and breeding of pathogen, and can with field planting of the Antagonistic Fungus on host,
To inhibit the occurrence degree of plant disease.
2, after the present invention is handled pear tree branch using gram lining institute series bacillus YY-1 fermentation liquid, it is inoculated with black rot of pear
Disease incidence is decreased obviously after bacterium, and lesion diameter significantly reduces, it was demonstrated that a gram lining institute series bacillus YY-1 can efficiently inhibit pears
Target spot pathogen bacterial strain has preferable biological control effect, has good effect, and the bacterial strain pair to prevention and treatment black rot of pear evil
Ecological environment is harmless, will not cause the drug resistance of cause of disease, and condition of culture is simple, is easy to save.
3, biocontrol agent provided by the invention has preparation simple, and at low cost, easy to industrialized production, control efficiency is good
It is good, and not will cause environmental pollution, pesticide can be replaced to prevent and treat black rot of pear, with good development and application prospects.
Detailed description of the invention
Fig. 1 is a gram colonial morphology figure of the lining institute series bacillus YY-1 in PDA culture medium;
Fig. 2 is a gram colonial morphology figure of the lining institute series bacillus YY-1 on LB culture medium;
Fig. 3 is a gram electron-microscope scanning figure of lining institute series bacillus YY-1;
Fig. 4 for gram lining institute series bacillus YY-1 16S rDNA sequential evolution analysis figure;
Fig. 5 is a gram influence of the lining institute series bacillus YY-1 fermentation liquid to black rot of pear bacterium JNT1111 hypha form;
Figure A is control group;Figure B is experimental group;
Fig. 6 grows black rot of pear bacterium mycelia for gram various concentration separation of fermentative broth supernatant of lining institute series bacillus
It influences;
Influence of the Fig. 7 for inoculation gram lining institute series bacillus YY-1 bacteria suspension to black rot of pear bacteria pathogenic.
Specific embodiment
Invention is further described in detail with attached drawing combined with specific embodiments below, but the scope of the present invention not office
It is limited to the content.Raw material described in embodiment is ordinary commercial products unless otherwise specified.Experiment as described in the examples
Method is without special instruction, i.e., routinely experimental methods of molecular biology operates.
The separation screening of 1 bacterial strain gram lining institute series bacillus YY-1 of embodiment and identification
Separation method: taking the cockscomb blade of collection health from Chongqing City Fuling District, and the pear tree shoot tissue cleaning of health is taken to disappear
Poison cuts the tissue that diameter is 5 mm, successively 45 s, 75% ethanol postincubation, 1 min is handled with sodium hypochlorite, at sterile water
1 min, sterile 1 min of water process are managed, is placed in after aseptic filter paper dries and is inoculated in 28 DEG C of PDA solid medium cultures.The plate
28 DEG C stationary culture 2-3 days.It is picked out according to the form, color, size of the bacterial clump screened on plate different thin
Bacterium is simultaneously transferred to PD fluid nutrient medium.
Screening technique: antagonistic bacterium is screened using opposite culture method, disinfection inoculation ring dips a small amount of bacterium solution, in PDA plate
Center scribing line, and the black rot of pear bacterium JNT1111 mycelia block for being 5 mm in two sides inoculation diameter, are placed in 28 DEG C of 5 d of culture, observe
Diameter between bacterium colony, every group repeats three times, only will be set as compareing in the plate of two sides inoculation JNT1111 mycelia block.To plate
On the bacterium with bacteriostatic activity that screens be transferred to PD fluid nutrient medium, purified, cultivated to get to the suppression of purifying
Active bacterial strain processed is named as YY-1.
Identification method: bacterial strain YY-1 is smooth, regular edges in PDA culture medium, intermediate in spherical milky outstanding
Bacterium colony (Fig. 1) is then smooth, edge sawtooth shape, flat white colony (Fig. 2) on LB solid medium.Bacterial strain YY-1's
Thallus is (Fig. 3) in the shape of a rod under a scanning electron microscope, can be changed bacterium for Gram's staining.
The DNA that bacterial strain YY-1 of the present invention is extracted with the bacteria total DNA of raw work biology Co., Ltd, then uses bacterium 16s
RDNA universal primer 16sF/R carries out PCR amplification, obtains amplified fragments, amplified fragments sequencing, particular sequence such as SEQ ID
Shown in NO.1.Sequence is compared through BLAST in GenBank, with a gram lining institute series bacillus (Paenibacillus kribbensis) affiliation is nearest.By to the 16s rDNA sequence construct in other representatives of YY-1 and bacillus genus
Phyletic evolution development tree, the results showed that YY-1 andPaenibacillus kribbensisRepresentative strain is gathered in same branch (figure
4) and be novel bacterial, bacterial strain of the present invention for a gram lining institute series bacillus (Paenibacillus kribbensis), it is named as
Gram lining institute series bacillus YY-1.
The application of 2 bacterial strain gram lining institute series bacillus YY-1 of embodiment
The preparation of separation of fermentative broth supernatant: with sterile PD fluid nutrient medium after gram lining institute series bacillus YY-1 is activated
Resuspension obtains bacteria suspension, then the bacteria suspension is inoculated in PD fluid nutrient medium with 5% inoculum concentration, in 180r/min, 28 DEG C
Supernatant is finally used 0.22 nm then by obtained fermentation liquid in 4 DEG C of 6000rpm centrifuging and taking supernatants by lower shaken cultivation 72h
Sterilised membrane filter filters up to the separation of fermentative broth supernatant, spare.
1, influence of the YY-1 to spore germination.
With aseptic water washing black rot of pear bacteria strain JNT1111 cirrus, spore liquid is adjusted to suitable in 40 power microscopes
Concentration.It takes isometric separation of fermentative broth supernatant and spore liquid to mix, draws 20 uL mixed liquors and glass slide center, be placed in nothing
In bacterium plate, 28 DEG C of 2.5 h of sprouting, as experimental group;Control group replaces separation of fermentative broth supernatant, other steps with sterile water
It is identical.It observes and records, calculates germination rate, as a result as shown in Figure 5.
It is obtained from microscopically observation: compared with the control group, bacterial strain can be caused after fermented liquid separation supernatant processing
The mycelia of JNT1111 grows deformity, mycelia shortened internodes, and has the phenomenon that expanding, and the separation of fermentative broth supernatant of 50% concentration
Liquid is 100% to the inhibiting rate of bacterial strain JNT1111 spore germination.
2, the antibacterial control effect testing of bacterial strain YY-1
PDA culture medium fusing is cooled to 40 ~ 50 DEG C, by separation of fermentative broth supernatant respectively with 0%, 2%, 3%, 4%, 5% ratio
The PDA plate that the supernatant of separation of fermentative broth containing various concentration is prepared in culture medium, the pears wheel for being then 5 mm by diameter is added
Line germ bacterial strain JNT1111 inoculated by hypha block observes and records hypha growth condition in above-mentioned PDA plate center, 28 DEG C of cultures.Knot
Fruit is as shown in Figure 6.
It can be seen from the figure that in the medium be added various concentration fermentation liquid after bacterial strain JNT1111 growth by
Different degrees of inhibition, and the higher bacterial strain JNT1111 growth inhibition of concentration of separation of fermentative broth supernatant is stronger, and bacterial strain
The growth of JNT1111 bacterium colony is irregular.And the strain growth of control group is rapid, bacterium colony growth is vigorous, gas natural disposition is strong.The result shows that bacterium
Antibacterial substance concentration in the separation of fermentative broth supernatant of strain YY-1 is higher, and Antibacterial Activity is stronger.
3, the living body control effect testing of bacterial strain YY-1
Intercepting pear tree branch fresh, healthy, that size and form is almost the same is material, and clear water is cleaned, and 75% alcohol disinfecting dries,
Paraffin sealing moisturizing punches its surface with the punch of disinfection, and experimental group is outstanding in the bacterium that wound is inoculated with 10 uL bacterial strain YY-1
Liquid (viable count about 1 × 105Cfu/mL after) drying, the black rot of pear bacteria strain JNT1111 mycelia block that inoculation diameter is 5 mm, bacterium
Silk faces wound, preservative film moisturizing;Control group replaces bacterial strain YY-1 bacteria suspension, other same experimental groups of step, experiment with sterile water
3 repetitions are set, and 28 DEG C of moisturizing culture 3d record wound incidence, as a result as shown in Figure 7.
From figure 7 it can be seen that after culture 3d, the black rot of pear when lesion diameter of experimental group (YY-1+JNT1111) and inoculation
The diameter change of bacteria strain JNT1111 mycelia block is unobvious, illustrates that experimental group is not fallen ill;And control group (PD+JNT1111)
Lesion diameter variation is more obvious, is about inoculated with 7 times of fungus block diameter, shows that bacterial strain YY-1 can be with Antagonistic Fungus gram lining institute class
Field planting of the bacillus on host.
To sum up, of the invention gram lining institute series bacillus (Paenibacillus kribbensis) YY-1 can efficiently press down
Black rot of pear bacteria strain processed has preferable biological control effect, and not will cause environmental pollution, pesticide can be replaced to pears wheel
Line disease is prevented and treated.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
SEQUENCE LISTING
<110>Yangtze Normal University;
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<170> SIPOSequenceListing 1.0
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<212> DNA
<213>gram lining institute series bacillus (Paenibacillus kribbensis)
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cctcaagcta cttgttcgac ttcaccccaa tcatctaccc caccttcggc ggctggctcc 60
ttgcggttac ctcaccgact tcgggtgttg taaactctcg tggtgtgacg ggcggtgtgt 120
acaagacccg ggaacgtatt caccgcggca tgctgatccg cgattactag caattccgac 180
ttcatgtagg cgagttgcag cctacaatcc gaactgagac cggcttttct aggattggct 240
ccacctcgcg ccttcgcttc ccgttgtacc ggccattgta gtacgtgtgt agcccaggtc 300
ataaggggca tgatgatttg acgtcatccc caccttcctc cggtttgtca ccggcagtct 360
gcttagagtg cccagcttga cctgctggca actaagcata agggttgcgc tcgttgcggg 420
acttaaccca acatctcacg acacgagctg acgacaacca tgcaccacct gtctcctctg 480
tcccgaagga aaggtctatc tctagaccgg tcaaagggat gtcaagacct ggtaaggttc 540
ttcgcgttgc ttcgaattaa accacatact ccactgcttg tgcgggtccc cgtcaattcc 600
tttgagtttc agtcttgcga ccgtactccc caggcggaat gcttaatgtg ttaacttcgg 660
caccaagggt atcgaaaccc ctaacaccta gcattcatcg tttacggcgt ggactaccag 720
ggtatctaat cctgtttgct ccccacgctt tcgcgcctca gcgtcagtta cagcccagag 780
agtcgccttc gccactggtg ttcctccaca tctctacgca tttcaccgct acacgtggaa 840
ttccactctc ctcttctgca ctcaagctcc ccagtttcca gtgcgacccg aagttgagcc 900
tcgggattaa acaccagact taaagagccg cctgcgcgcg ctttacgccc aataattccg 960
gacaacgctt gccccctacg tattaccgcg gctgctggca cgtagttagc cggggctttc 1020
ttctcaggta ccgtcacttc aagagcagtt actctatcaa gcgttcttcc ctggcaacag 1080
agctttacga tccgaaaacc ttcatcactc acgcggcgtt gctccgtcag gctttcgccc 1140
attgcggaag attccctact gctgcctccc gtaggagtct gggccgtgtc tcagtcccag 1200
tgtggccgat caccctctca ggtcggctac gcatcgtcgc cttggtaggc ctttacccca 1260
ccaactagct aatgcgccgc aggcccatcc acaagtgaca gattgctccg cctttcctcc 1320
ttctcccatg caggaaaagg atgtatcggg tattagctac cgtttccggt agttatccct 1380
gtcttgtggg caggttgcct acgtgttact cacccgtccg ccgc 1424
Claims (8)
1. one kind gram lining institute series bacillus (Paenibacillus kribbensis) YY-1, during the bacterial strain is preserved in
State's Type Tissue Collection, deposit number are CCTCC NO. M2019242.
2. application of the as described in claim 1 gram of lining institute series bacillus YY-1 in prevention and treatment trunks of fruit trees disease fungus.
3. applying according to claim 2, which is characterized in that the disease fungus is black rot of pear bacterium.
4. a kind of biocontrol agent, it is characterised in that: effective component is described in claim 1 grams of lining institute series bacillus YY-1.
5. biocontrol agent according to claim 4, which is characterized in that described gram of lining institute series bacillus YY-1 is the bacterial strain
Culture bacteria suspension, fermentation liquid or fermentation separation supernatant.
6. biocontrol agent according to claim 4, which is characterized in that gram lining institute class in the fermentation liquid and culture bacteria suspension
The viable count of bacillus YY-1 is not less than 1 × 105cfu/mL。
7. biocontrol agent according to claim 4, which is characterized in that the separation of fermentative broth supernatant uses following steps system
: it will be resuspended to obtain bacteria suspension with sterile PD fluid nutrient medium after gram lining institute series bacillus YY-1 activation, then by the bacterium
Suspension is inoculated in PD fluid nutrient medium with 5% inoculum concentration, at 120~200r/min, 25~30 DEG C shaken cultivation 48h~
72h, it is finally that supernatant is sterile using 0.22 nm then by obtained fermentation liquid in 4 DEG C of 6000 ~ 8000rpm centrifuging and taking supernatants
Membrane filtration is up to the separation of fermentative broth supernatant.
8. biocontrol agent as claimed in claim 4 prevention and treatment ring rot of apple, black rot of pear, pears dry rot, Kiwi berry branch-rot,
Application in grape limb canker or blueberry branch-rot.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910380414.4A CN110157638B (en) | 2019-05-08 | 2019-05-08 | Paenibacillus kribbensis YY-1 and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201910380414.4A CN110157638B (en) | 2019-05-08 | 2019-05-08 | Paenibacillus kribbensis YY-1 and application thereof |
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Cited By (4)
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CN111793498A (en) * | 2020-07-31 | 2020-10-20 | 福建三炬生物科技股份有限公司 | Microbial agent and preparation method and application thereof |
CN112481160A (en) * | 2020-11-27 | 2021-03-12 | 江苏省农业科学院 | Paenibacillus polymyxa and application thereof in prevention and control of pear diseases |
CN115725419A (en) * | 2022-09-22 | 2023-03-03 | 贵州大学 | Phosphorus-dissolving blueberry endophytic trichoderma and application thereof |
CN115725452A (en) * | 2022-09-21 | 2023-03-03 | 山东佰渥康生物科技有限公司 | Paenibacillus atrophaeus, microbial inoculum and application thereof |
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CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
WO2016020371A1 (en) * | 2014-08-04 | 2016-02-11 | Basf Se | Antifungal paenibacillus strains, fusaricidin-type compounds, and their use |
WO2017137351A1 (en) * | 2016-02-09 | 2017-08-17 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
WO2017137353A1 (en) * | 2016-02-09 | 2017-08-17 | Basf Se | Mixtures and compositions comprising paenibacillus strains or metabolites thereof and other biopesticides |
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CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
WO2016020371A1 (en) * | 2014-08-04 | 2016-02-11 | Basf Se | Antifungal paenibacillus strains, fusaricidin-type compounds, and their use |
WO2017137351A1 (en) * | 2016-02-09 | 2017-08-17 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
WO2017137353A1 (en) * | 2016-02-09 | 2017-08-17 | Basf Se | Mixtures and compositions comprising paenibacillus strains or metabolites thereof and other biopesticides |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN111793498A (en) * | 2020-07-31 | 2020-10-20 | 福建三炬生物科技股份有限公司 | Microbial agent and preparation method and application thereof |
CN112481160A (en) * | 2020-11-27 | 2021-03-12 | 江苏省农业科学院 | Paenibacillus polymyxa and application thereof in prevention and control of pear diseases |
CN115725452A (en) * | 2022-09-21 | 2023-03-03 | 山东佰渥康生物科技有限公司 | Paenibacillus atrophaeus, microbial inoculum and application thereof |
CN115725419A (en) * | 2022-09-22 | 2023-03-03 | 贵州大学 | Phosphorus-dissolving blueberry endophytic trichoderma and application thereof |
CN115725419B (en) * | 2022-09-22 | 2024-02-06 | 贵州大学 | Phosphorus-dissolving blueberry endophytic trichoderma and application thereof |
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