CN110146612A - A kind of method of cyanide ion concentration in measurement solution - Google Patents
A kind of method of cyanide ion concentration in measurement solution Download PDFInfo
- Publication number
- CN110146612A CN110146612A CN201910441050.6A CN201910441050A CN110146612A CN 110146612 A CN110146612 A CN 110146612A CN 201910441050 A CN201910441050 A CN 201910441050A CN 110146612 A CN110146612 A CN 110146612A
- Authority
- CN
- China
- Prior art keywords
- solution
- sample
- cyanocobalamin
- hydroxycobalamin
- cyanide ion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
A kind of method of cyanide ion concentration in measurement solution, the characteristic of cyanocobalamin is combined into using cyanide ion in hydroxycobalamin and its salt and solution, pass through the concentration of cyanocobalamin in high performance liquid chromatography detection solution, the concentration of cyanide ion in solution, calculation formula are calculated with this again are as follows: C2=(C1S2D26)/(S11355).The present invention using hydroxycobalamin and its salt in the solution can affine cyanide ion, the property of stable cyanocobalamin is combined into cyanide ion, and by the concentration of cyanocobalamin in high performance liquid chromatography detection solution, to calculate the concentration of cyanide ion.Only need to prepare the standard solution of cyanocobalamin in continuous mode, it is not necessary to prepare cyanide standard solution.Measurement is quickly, safe, and the cyanide ion concentration range of measurement is wide.
Description
Technical field
The present invention relates to a kind of methods of cyanide ion concentration in measurement solution, belong to chemical analysis technology field.
Background technique
Free cyanide is a kind of extremely toxic substance, is one of environmental monitoring and food analysis essential items for inspection.Country is about " raw
Sanitary standard for drinking water living " in the limitation of regulation concentration of cyanide be 0.05mg/L, country is about " Industrial " three Waste " discharge is tentative
Standard " allow concentration of emission to be 0.5mg/L (with Cyanide ionometer) cyanide highest in industrial wastewater.
The method of concentration of cyanide mainly has volumetric method and spectrophotometry (HJ484-2009) in measurement solution at present.Nothing
By being all easy interference by other ions or coloring matter of volumetric method or spectrophotometry, the rate of recovery is unstable, measurement
Result error is larger.Spectrophotometry is distilled when measuring, and cyanide is easy to decompose during heating, causes to survey
Determine unstable result.Also, it is needed in spectrophotometry continuous mode using extremely toxic substances such as cyanide and pyridines, this also gives reality
It tests room and brings security risk.
Summary of the invention
The present invention is directed to prior art problem, provides a kind of method for measuring cyanide ion concentration in solution, utilizes hydroxyl cobalt
Amine and its salt in the solution can affine cyanide ion, the property of stable cyanocobalamin is combined into cyanide ion, and pass through height
Effect liquid phase chromatogram detects the concentration of cyanocobalamin in solution, to calculate the concentration of cyanide ion.It only needs to match in continuous mode
The standard solution of cyanocobalamin processed, it is not necessary to prepare cyanide standard solution.Measurement is quickly, safe, the cyanide ion concentration model of measurement
It encloses wide.
Technical problem of the present invention is solved with following technical solution:
A kind of method of cyanide ion concentration in measurement solution is combined into cyanogen using cyanide ion in hydroxycobalamin and its salt and solution
The characteristic of cobalt amine, by the concentration of cyanocobalamin in high performance liquid chromatography detection solution, then with this come calculate in solution cyanogen root from
The concentration of son, calculation formula are as follows:
C2=(C1S2D26)/(S11355)
In formula: C1 --- cyanocobalamin standard concentration after dilution, mg/L；
S1 --- cyanocobalamin standard items peak area；
S2 --- the peak area of cyanocobalamin in sample to be tested；
C2 --- cyanide ion concentration, mg/L in solution to be measured；
26 --- the molal weight of cyanide ion (CN-), g/mol；
1355 --- the molal weight of cyanocobalamin, g/mol；
D --- the extension rate of solution to be measured.
The method of cyanide ion concentration in said determination solution, the configuration of the high performance liquid chromatography detection process and solution
Include the following steps:
A, solution pre-treatment and the configuration of hydroxycobalamin solution to be measured: solution to be measured is filtered clarification using filter paper, and adjust to
Survey solution ph is 3-8；
It prepares hydroxycobalamin concentrated solution: weighing hydroxycobalamin or hydroxocobalamine salt, diluted with deionized water, the weight of hydroxycobalamin or hydroxocobalamine salt
The ratio between amount and the volume of deionized water are 0.4g:10 mL；
It prepares hydroxycobalamin weak solution: weighing hydroxycobalamin or hydroxocobalamine salt, diluted with deionized water, the weight of hydroxycobalamin or hydroxocobalamine salt
The ratio between amount and the volume of deionized water are 0.4g:1000 mL；
B, prepared by sample to be tested: in solution to be measured prepared by sample to be tested of the cyanide ion concentration lower than 0.1mg/L: 100mL being taken to hold
The hydroxycobalamin concentrated solution that step a described in 1mL is prepared is added, with solution constant volume to be measured, 40 DEG C of water-baths in measuring bottle solution rinse to be measured
60min；
In solution to be measured prepared by sample to be tested of the cyanide ion concentration higher than 0.1mg/L: taking the volumetric flask of a constant volume, Xiang Rongliang
The hydroxycobalamin weak solution of 3/4 volume of volumetric flask is added in bottle, 1mL solution to be measured is added, with deionized water or hydroxycobalamin weak solution
Constant volume, 40 DEG C of 60 min of water-bath；
Sample to be tested is replaced to configure blank sample with deionized water；
C, it sample detection: is detected using high performance liquid chromatography:
Chromatographic condition: chromatographic column is Inertsil ODS-3 4.6*250mm 5um, and buffer solution is the phosphoric acid of 0.05mmol/L
Potassium dihydrogen, pH value 3.0, organic phase are acetonitrile, and the volume ratio of acetonitrile and buffer solution is 14:86, flow velocity 1mL/min, inspection
Survey wavelength 361nm, sample volume 20ul；
Cyanocobalamin standard items are prepared: the content that is provided according to cyanocobalamin standard items, moisture first prepare the cyanocobalamin standard of 100mg/L
Cyanocobalamin standard items concentrated solution is diluted 10-25 times before detection by product concentrated solution, until concentration is 4-10mg/L；
Detection: standard items benefit after the cyanocobalamin dilution of the blank sample, sample to be tested and the step c preparation that respectively prepare step b
It is detected with high performance liquid chromatography, and carries out atlas analysis, as a result calculated.
The method of cyanide ion concentration in said determination solution, in the step a, solution ph to be measured by hydrochloric acid or
Sodium hydroxide is adjusted.
The method of cyanide ion concentration in said determination solution, in the step b, cyanide ion concentration is high in solution to be measured
In the sample preparation of 0.1mg/L, selecting volume according to the hydroxycobalamin residual area percentage in the sample to be tested of detection is 25-
The volumetric flask of 500mL guarantees that hydroxycobalamin residual area percentage is 10% or more to adjust the extension rate of sample to be tested.
The method of cyanide ion concentration in said determination solution, in the step c, the dilution of cyanocobalamin standard items concentrated solution
Multiple is according to the appearance rea adjusting of sample, and peak area and extension rate are in inverse ratio out, connects the peak area of cyanocobalamin standard items
Nearly sample peak area.
The present invention is using hydroxycobalamin and its salt (including hydrochloric acid hydroxycobalamin, Hydroxocobalamine Acetate, sulfuric acid hydroxycobalamin etc.) in solution
In affine cyanide ion, the characteristic for stablizing nontoxic cyanocobalamin can be combined into cyanide ion, is examined by high performance liquid chromatography
The concentration for surveying cyanocobalamin in solution, the concentration of cyanide ion is calculated with this.Cyanogen of the measuring method of the present invention in solution to be measured
When ion concentration is 0.01mg/L, the characteristic absorption peak of the cyanocobalamin measured is still it is obvious that can satisfy detection completely needs
It asks；Only need to use hydroxycobalamin and its a kind of reagent of salt in sample handling processes, it is easy to operate；It is only needed to configure in continuous mode
The standard solution of cyanocobalamin, without preparing hydride solution, continuous mode is safer.
Detailed description of the invention
Fig. 1 is cyanocobalamin standard chromatogram；
Fig. 2 is the chromatogram for the sample to be tested that cyanide ion concentration is 0.01mg/L in solution to be measured；
Fig. 3 is the comparison diagram of the standard items and solution to be measured after cyanocobalamin dilution.
Specific embodiment
The present invention is capable of the characteristic of affine cyanide ion, hydroxycobalamin and its salt and cyanogen using hydroxycobalamin and its salt in the solution
Radical ion generates stable cyanocobalamin with the ratio reaction of 1:1, is diluted using high performance liquid chromatography detection sample to be tested, cyanocobalamin
The appearance situation of standard items and blank sample afterwards, cyanocobalamin standard chromatogram are for calculating cyanide ion concentration, simultaneously
Also it can show the appearance time of cyanocobalamin, cyanocobalamin standard items concentrated solution be prepared, according to sample peak rea adjusting cyanocobalamin mark
The extension rate of quasi- product concentrated solution guarantees that cyanocobalamin standard items peak area close to sample peak area, reduces detection error；Blank sample
Product are for compareing hydroxycobalamin appearance time；Sample to be tested mainly sees that cyanocobalamin goes out peak position and peak area, while obtaining hydroxyl cobalt
The area percentage of amine guarantees cyanocobalamin or hydroxocobalamine salt mistake in hydroxycobalamin or hydroxocobalamine salt and cyanide ion reaction process
Amount.Hydroxycobalamin content is too low, then increases solution extension rate to be measured, guarantee remaining hydroxycobalamin peak area percent be 10% with
On.Change the volume of volumetric flask or changes the additional amount of solution to be measured to adjust the extension rate of solution to be measured.To what is obtained
Chromatogram is analyzed and is calculated, and according to cyanocobalamin, concentration and peak area are linear in liquid chromatogram: C1/S1=to be measured
Cyanocobalamin concentration/S2 in sample, in solution to be measured in cyanide ion 2=sample to be tested of concentration C cyanocobalamin concentration × molecular weight it
Than (26/1355), according to the concentration of cyanocobalamin standard concentration and calculated by peak area cyanide ion, calculation formula are as follows:
C2=(C1S2D26)/(S11355)
In formula: C1 --- the cyanocobalamin standard concentration after dilution, mg/L；
S1 --- cyanocobalamin standard items peak area；
S2 --- the peak area of cyanocobalamin in sample to be tested；
C2 --- cyanide ion concentration, mg/L in solution to be measured；
26 --- the molal weight of cyanide ion (CN-), g/mol；
1355 --- the molal weight of cyanocobalamin, g/mol；
D --- the extension rate of solution to be measured.
The method of the present invention is described in further detail combined with specific embodiments below and is verified the linear of the method and
Repeatability.
Embodiment 1
Verifying explanation is carried out to the linear relationship of the method for the present invention.
(1) take demarcated concentration be 87mg/L potassium cyanide standard solution 100mL it is stand-by, conversion cyanide ion concentration be
34.8mg/L。
(2) 0.40g Hydroxocobalamine Acetate is weighed, is added in 1000mL beaker, with deionized water dissolving, is diluted to 1000mL.
(3) potassium cyanide calibration liquid is diluted 5,10,20,50 times respectively and is used as solution to be measured.
(4) 5 100mL volumetric flasks are taken, the vinegar that the step 2 of 3/4 volume of volumetric flask is prepared is separately added into each volumetric flask
1mL deionized water is added wherein being separately added into 1mL solution to be measured in four volumetric flasks in sour hydroxycobalamin solution in one volumetric flask
As blank sample, 5 volumetric flasks use deionized water constant volume, 40 DEG C of water-bath 60min.
(5) by high concentration cyanocobalamin standard solution (100.4mg/L) dilute 25 times to concentration be 4.02mg/L.
(6) aforementioned four sample to be tested, blank sample and diluted cyanocobalamin standard items are analyzed with liquid chromatogram
It calculates, as a result such as the following table 1.
The calculated cyanide ion concentration of 1 the method for the present invention of table and theoretical reduced value contrast table
| Sample ID | Standard items | Blank | 5 times of dilution | 10 times of dilution | 20 times of dilution | 50 times of dilution |
| Calculate cyanide ion concentration | —— | 0 mg/L | 6.96 mg/L | 3.48 mg/L | 1.74 mg/L | 0.696mg/L |
| Sample peak area | 87014 | 0 | 77786 | 38806 | 19499 | 7746 |
| Convert cyanide ion concentration | —— | 0 mg/L | 6.90mg/L | 3.44mg/L | 1.72mg/L | 0.69mg/L |
Referring to table 1, the relativeness of the calculated cyanide ion concentration of measuring method of the present invention and sample peak area obtains R2=1,
It can be seen that cyanide ion concentration and cyanocobalamin peak area linear relationship are good in the method for the present invention.
Embodiment 2
Verifying explanation is carried out to the repeatability of the method for the present invention, cyanide ion concentration is lower than 0.1mg/L in sample to be tested.
(1) take demarcated concentration be 87mg/L potassium cyanide standard solution 100mL it is stand-by, conversion cyanide ion concentration be
34.8mg/L。
(2) 0.40g hydrochloric acid hydroxycobalamin is weighed, with deionized water dissolving, is diluted to 10mL, it is dense molten that hydrochloric acid hydroxycobalamin is made
Liquid.
(3) potassium cyanide calibration liquid is first diluted 1000 times and is used as solution to be measured.
(4) 5 100mL volumetric flasks are taken, with solution rinse to be measured, wherein being separately added into 1mL hydrochloric acid hydroxyl cobalt in 4 volumetric flasks
Amine concentrated solution, in addition 1mL deionized water is added in 1 volumetric flask, and 5 volumetric flasks use solution constant volume to be measured, 40 DEG C of water-baths
60min。
(5) by high concentration cyanocobalamin standard solution (100.4mg/L) dilute 25 times to concentration be 4.02mg/L.
(6) aforementioned four sample to be tested, blank sample and diluted cyanocobalamin standard items are analyzed with liquid chromatogram
It calculates, as a result such as the following table 2.
The repetitive test result of 2 present invention measurement low concentration cyanide ion of table
| Sample ID | Standard items | Blank | Sample 1 | Sample 2 | Sample 3 | Sample 4 |
| Calculate cyanide ion concentration | —— | 0 mg/L | 0.0348mg/L | 0.0348mg/L | 0.0348mg/L | 0.0348mg/L |
| Sample peak area | 87089 | 0 | 38536 | 38508 | 38585 | 38569 |
| Convert cyanide ion concentration | —— | 0 | 0.0345mg/L | 0.0345mg/L | 0.0345mg/L | 0.0345mg/L |
Embodiment 3
Sample to be tested cyanide ion concentration is higher than 0.1mg/L, measuring method repeatability verifying of the present invention.
(1) take demarcated concentration be 87mg/L potassium cyanide standard solution 100mL it is stand-by, conversion cyanide ion concentration be
34.8mg/L。
(2) 0.40g hydroxycobalamin is weighed, is added in 1000mL beaker, with deionized water dissolving, is diluted to 1000mL.
(3) potassium cyanide calibration liquid is first diluted 10 times and is used as solution to be measured.
(4) take 5 100mL volumetric flasks that the hydroxycobalamin solution of 3/4 volume of volumetric flask is added, wherein 4 volumetric flasks add respectively
Enter 1mL solution to be measured, in addition 1mL deionized water is added in 1 volumetric flask, and 5 volumetric flasks use deionized water constant volume, 40 DEG C of water-baths
60min。
(5) high concentration cyanocobalamin standard solution (100.4mg/L) is diluted 25 times to 4.02mg/L.
(6) aforementioned four sample to be tested, blank sample and diluted cyanocobalamin standard items are analyzed with liquid chromatogram
It calculates, as a result such as the following table 3.
3 cyanide ion concentration of table is higher than the sample to be tested repeatability verification result of 0.1mg/L
| Sample ID | Standard items | Blank | Sample 1 | Sample 2 | Sample 3 | Sample 4 |
| Calculate cyanide ion concentration | —— | 0 mg/L | 3.48mg/L | 3.48mg/L | 3.48mg/L | 3.48mg/L |
| Sample peak area | 87056 | 0 | 38925 | 38985 | 38951 | 38946 |
| Convert cyanide ion concentration | —— | 0 | 3.449mg/L | 3.454mg/L | 3.451mg/L | 3.451mg/L |
Referring to table 2 and table 3, measurement cyanide ion concentration is lower than the sample to be tested of 0.1mg/L in table 2, and the relative standard measured is inclined
Difference is 0, and table 3 is the sample to be tested for measuring cyanide ion concentration and being higher than 0.1mg/L, and the relative standard deviation measured is 0.05%, is said
The accuracy of the cyanide ion solution of the low concentration and high concentration of bright measuring method measurement of the present invention is all very high, fully achieves
Analysis requires.
Referring to Fig. 1 and Fig. 2, in the high-efficient liquid phase chromatogram of cyanocobalamin standard items, the appearance time of cyanocobalamin is 8.8min；
Cyanide ion concentration be 0.01mg/L sample to be tested chromatogram in, appearance time is at 3min be hydroxycobalamin chromatographic peak,
It is the chromatographic peak of cyanocobalamin at 8.8min, matches with the appearance time of cyanocobalamin in cyanocobalamin standard items, and the spy of cyanocobalamin
Absorption peak is levied it is obvious that being much higher than baseline noise, can be used for quantitative detection.
Referring to Fig. 3, chromatographic line a is cyanocobalamin standard items, and chromatographic line b is sample to be tested, it can be seen that cyanogen in sample to be tested
The appearance time of cobalt amine is identical with cyanocobalamin appearance time in standard items.
Claims (5)
1. a kind of method of cyanide ion concentration in measurement solution, it is characterised in that: utilize cyanogen in hydroxycobalamin and its salt and solution
Radical ion is combined into the characteristic of cyanocobalamin, calculates by the concentration of cyanocobalamin in high performance liquid chromatography detection solution, then with this
Out in solution cyanide ion concentration, calculation formula are as follows:
C2=(C1S2D26)/(S11355)
In formula: C1 --- cyanocobalamin standard concentration after dilution, mg/L；
S1 --- cyanocobalamin standard items peak area；
S2 --- the peak area of cyanocobalamin in sample to be tested；
C2 --- cyanide ion concentration, mg/L in solution to be measured；
26 --- the molal weight of cyanide ion (CN-), g/mol；
1355 --- the molal weight of cyanocobalamin, g/mol；
D --- the extension rate of solution to be measured.
2. the method for cyanide ion concentration in measurement solution according to claim 1, it is characterised in that: the efficient liquid phase
The configuration of chromatography detection process and solution includes the following steps:
A, solution pre-treatment and the configuration of hydroxycobalamin solution to be measured: solution to be measured is filtered clarification using filter paper, and adjust to
Survey solution ph is 3-8；
It prepares hydroxycobalamin concentrated solution: weighing hydroxycobalamin or hydroxocobalamine salt, diluted with deionized water, the weight of hydroxycobalamin or hydroxocobalamine salt
The ratio between amount and the volume of deionized water are 0.4g:10 mL；
It prepares hydroxycobalamin weak solution: weighing hydroxycobalamin or hydroxocobalamine salt, diluted with deionized water, the weight of hydroxycobalamin or hydroxocobalamine salt
The ratio between amount and the volume of deionized water are 0.4g:1000 mL；
B, prepared by sample to be tested: in solution to be measured prepared by sample to be tested of the cyanide ion concentration lower than 0.1mg/L: 100mL being taken to hold
The hydroxycobalamin concentrated solution that step a described in 1mL is prepared is added, with solution constant volume to be measured, 40 DEG C of water-baths in measuring bottle solution rinse to be measured
60min；
In solution to be measured prepared by sample to be tested of the cyanide ion concentration higher than 0.1mg/L: taking the volumetric flask of a constant volume, Xiang Rongliang
The hydroxycobalamin weak solution of 3/4 volume of volumetric flask is added in bottle, 1mL solution to be measured is added, with deionized water or hydroxycobalamin weak solution
Constant volume, 40 DEG C of 60 min of water-bath；
Sample to be tested is replaced to configure blank sample with deionized water；
C, it sample detection: is detected using high performance liquid chromatography:
Chromatographic condition: chromatographic column is Inertsil ODS-3 4.6*250mm 5um, and buffer solution is the phosphoric acid of 0.05mmol/L
Potassium dihydrogen, pH value 3.0, organic phase are acetonitrile, and the volume ratio of acetonitrile and buffer solution is 14:86, flow velocity 1mL/min, inspection
Survey wavelength 361nm, sample volume 20ul；
Cyanocobalamin standard items are prepared: the content that is provided according to cyanocobalamin standard items, moisture first prepare the cyanocobalamin standard of 100mg/L
Cyanocobalamin standard items concentrated solution is diluted 10-25 times before detection by product concentrated solution, until concentration is 4-10mg/L；
Detection: standard items benefit after the cyanocobalamin dilution of the blank sample, sample to be tested and the step c preparation that respectively prepare step b
It is detected with high performance liquid chromatography, and carries out atlas analysis, as a result calculated.
3. the method for cyanide ion concentration in measurement solution according to claim 2, it is characterised in that: in the step a,
Solution ph to be measured is adjusted by hydrochloric acid or sodium hydroxide.
4. the method for cyanide ion concentration in measurement solution according to claim 3, it is characterised in that: in the step b,
It is surplus according to the hydroxycobalamin in the sample to be tested of detection in solution to be measured in sample preparation of the cyanide ion concentration higher than 0.1mg/L
Remaining area percentage selects the volumetric flask of 25-500mL volume, to adjust the extension rate of sample to be tested, guarantees that hydroxycobalamin is remaining
Area percentage is 10% or more.
5. the method for cyanide ion concentration in measurement solution according to claim 4, it is characterised in that: in the step c,
The extension rate of cyanocobalamin standard items concentrated solution is according to the appearance rea adjusting of sample, and peak area and extension rate are in inverse ratio out,
Make the peak area of cyanocobalamin standard items close to sample peak area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910441050.6A CN110146612A (en) | 2019-05-24 | 2019-05-24 | A kind of method of cyanide ion concentration in measurement solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910441050.6A CN110146612A (en) | 2019-05-24 | 2019-05-24 | A kind of method of cyanide ion concentration in measurement solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110146612A true CN110146612A (en) | 2019-08-20 |
Family
ID=67593245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910441050.6A Pending CN110146612A (en) | 2019-05-24 | 2019-05-24 | A kind of method of cyanide ion concentration in measurement solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110146612A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11324771B2 (en) * | 2020-02-12 | 2022-05-10 | RK Pharma Solutions LLC | Process for the preparation of hydroxocobalamin hydrochloride |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4300907A (en) * | 1980-02-04 | 1981-11-17 | Becton, Dickinson And Company | Serum vitamin B12 assay and kit therefor |
| GB2084320A (en) * | 1980-09-22 | 1982-04-07 | Amersham Int Ltd | Assay of vitamin B12 |
| US4333918A (en) * | 1979-03-23 | 1982-06-08 | Technicon Instruments Corporation | Radioassay for vitamin B12 |
| CN101538599A (en) * | 2008-03-21 | 2009-09-23 | 华东理工大学 | Method for improving yield of denitrified pseudomonas vitamin B12 |
| CN103623754A (en) * | 2012-08-24 | 2014-03-12 | 河北华荣制药有限公司 | Light reaction device and applications in preparation of hydroxycobalamin or salts thereof |
-
2019
- 2019-05-24 CN CN201910441050.6A patent/CN110146612A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4333918A (en) * | 1979-03-23 | 1982-06-08 | Technicon Instruments Corporation | Radioassay for vitamin B12 |
| US4300907A (en) * | 1980-02-04 | 1981-11-17 | Becton, Dickinson And Company | Serum vitamin B12 assay and kit therefor |
| GB2084320A (en) * | 1980-09-22 | 1982-04-07 | Amersham Int Ltd | Assay of vitamin B12 |
| CN101538599A (en) * | 2008-03-21 | 2009-09-23 | 华东理工大学 | Method for improving yield of denitrified pseudomonas vitamin B12 |
| CN103623754A (en) * | 2012-08-24 | 2014-03-12 | 河北华荣制药有限公司 | Light reaction device and applications in preparation of hydroxycobalamin or salts thereof |
Non-Patent Citations (5)
| Title |
|---|
| A. CRUZ-LANDEIRA ET AL: "A New Spectrophotometric Method for the Toxicological Diagnosis of Cyanide Poisoning", 《JOURNAL OF ANALYTICAL TOXICOLOGY》 * |
| M. LAFORGE ET AL: "A Rapid Spectrophotometric Blood Cyanide Determination Applicable to Emergency Toxicology", 《JOURNAL OF ANALYTICAL TOXICOLOGY》 * |
| P HOUETO ET AL: "Relation of blood cyanide to plasma cyanocobalamin concentration after a fixed dose of hydroxocobalamin in cyanide poisoning", 《THE LANCET》 * |
| 吴琼珠 等: "HPLC测定甲钴胺的有关物质", 《南京军医学院学报》 * |
| 陈乔: "HPLC测定腺苷钴胺有关物质", 《河北化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11324771B2 (en) * | 2020-02-12 | 2022-05-10 | RK Pharma Solutions LLC | Process for the preparation of hydroxocobalamin hydrochloride |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104569256B (en) | A kind of method adopting 3 kinds of OIT antiseptics in liquid chromatography tandom mass spectrometry determination water base adhesive | |
| CN108896504B (en) | Method for simultaneously determining nitrate content of p-nitrophenol and degradation product thereof | |
| CN106324144A (en) | Method for detecting chlorate, perchlorate and bromate from milk powder and infant formula milk powder by hydrophilic interaction chromatography-tandem mass spectrometry | |
| CN105021608A (en) | Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit | |
| CN101256138A (en) | Method for determining soil total cyanide--isonicotinic acid--barbituric acid spectrophotometric method | |
| CN103529117B (en) | The measuring method of cobalt contents in a kind of tobacco lining paper | |
| CN107561172B (en) | Method for simultaneously detecting content of multiple vitamins in nutrient soft capsule | |
| CN104849358A (en) | Optimization method for parameters of analytical instrument used for analysis of forms of elemental arsenic based on HPLC-HG-AFS system | |
| Chen et al. | Online spectrophotometric determination of Fe (II) and Fe (III) by flow injection combined with low pressure ion chromatography | |
| Komárek et al. | ET-AAS determination of aluminium in dialysis concentrates after continuous flow solvent extraction | |
| CN110146612A (en) | A kind of method of cyanide ion concentration in measurement solution | |
| CN101122587A (en) | Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco | |
| Beltrán-Martinavarro et al. | Quantification of melamine in drinking water and wastewater by micellar liquid chromatography | |
| CN111189956B (en) | H 2 O 2 Method for detecting content of nitrite in sodium chloride sample by using oxidized ion chromatography | |
| CN108760920A (en) | A method of cyazofamid and its metabolite residue amount are measured based on HPLC-MSMS methods | |
| Shrivas | Monitoring of copper level in water and soil samples by using liquid–liquid extraction | |
| CN110927091A (en) | Method for quantitatively detecting piperazine water solution | |
| CN105223143B (en) | A kind of method of pressure break liquid hold-up in measure oilfield sewage | |
| Gong et al. | An optosensor for riboflavin with C18 silica gel as a substrate | |
| Semaan et al. | Spectrophotometric determination of furosemide based on its complexation with Fe (III) in ethanolic medium using a flow injection procedure | |
| CN109696504A (en) | Method that is a kind of while detecting multiple water-soluble vitamin in food | |
| KR101350081B1 (en) | the method for detecting high concentration of phosphate | |
| Blaser et al. | Metal binding properties of leaf litter extracts: I. Discontinuous spectrophotometric titration with iron and copper | |
| CN102053120A (en) | Method for measuring content of cyclohexanone-oxime by liquid chromatography | |
| CN108414467A (en) | The ultraviolet specrophotometer assay method of sodium dimethyl dithiocarbamate content in a kind of solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |