CN110132702A - A kind of embedding method for particular pathologies tissue - Google Patents
A kind of embedding method for particular pathologies tissue Download PDFInfo
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- CN110132702A CN110132702A CN201910500511.2A CN201910500511A CN110132702A CN 110132702 A CN110132702 A CN 110132702A CN 201910500511 A CN201910500511 A CN 201910500511A CN 110132702 A CN110132702 A CN 110132702A
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- tissue
- hole
- wax stone
- embedding
- wax
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of embedding methods for particular pathologies tissue, the wax stone with hole is prepared first, tissue is put into hole when embedding, wax liquor filling pore is embedded, hole can limit moving and preventing it from toppling over for tissue block, play the role of fixing organization direction, convenient for user slice when switch to desired part, while can effectively avoid because with tweezers repeatedly clamping tissue adjust its towards when caused by tissue mechanical damage.
Description
Technical field
The present invention relates to pathological tissue processing technology field, in particular to a kind of embedding side for particular pathologies tissue
Method.
Background technique
Histopathologic examination is a kind of inspection method based on microsection technology of preparing, due to body tissue quality pine
It is soft, it is difficult to be efficiently sliced, and more difficult acquisition meets the ultra-thin section of microexamination, it is therefore desirable to use some supports
The production being sliced again after the embedding medium of effect, such as paraffin filling tissue cellularity, this slice are referred to as " paraffin
Slice ", for paraffin embedding, the method generallyd use is poured the paraffin of melting into embedding frame, before paraffin
It is put into paraffin with tissue block of the tweezers clamping after transparent and waxdip step process, and adjusts the direction of tissue block with tweezers,
Desired part is switched to when to be sliced, and behind the direction for adjusting tissue block, allows wax stone to condense in room temperature, or be put into cold water and accelerate
It is solidified；Embedding frame is removed after wax stone solidification takes out the subsequent sectioning of wax stone progress.At least have three in the process
A factor influences the quality and efficiency of organization embedding:
First, adjust tissue block embedding towards when need to move repeatedly the position or orientation of tissue block, and paraffin is at room temperature
It can solidify quickly, it may appear that do not adjust the phenomenon that tissue block has just been solidified towards wax liquor, cause slice that cannot switch to reason
Think position；Second, allowing to adjust direction before paraffin, since tissue block lacks auxiliary in the paraffin of melting
Support is also easy that positional shift occurs before wax liquor solidifies or topples over, especially some tiny pipe-cavity-like tissues or sheet
Tissue；Third, needing clamping tissue repeatedly when adjusting tissue block position or orientation with tweezers, the mechanicalness damage of tissue will cause
Wound, it is possible to will affect subsequent observation and judgement to lesion tissue.
Summary of the invention
In view of above-mentioned problem, the present invention proposes a kind of embedding method for particular pathologies tissue.
Particular content is as follows: a kind of embedding method for particular pathologies tissue, it is characterized in that: this method includes following step
It is rapid:
1. production has the wax stone of hole；
2. embedding, the wax stone made is put on platform, the lumen shape of quasi- embedding or sheet pathological tissue are put into wax stone
In hole, position and angle with tweezers adjustment pathological tissue make the destination region for intending being sliced in pathological tissue close to wax stone
The bottom of hole finally fills wax liquor into hole and carries out organization embedding；
3. the wax stone for being embedded with pathological tissue is adhered on platform wood and is sliced after intrapore wax liquor solidifies completely.
Preferably, the step 1. in wax stone hole the middle part of wax stone is set.
Preferably, the step 1. in the cross sectional shape of wax stone hole be circle, pipe-cavity-like pathological tissue was using should
The wax stone of kind hole is embedded.
Preferably, the step 1. in wax stone hole cross sectional shape be ellipse or rectangle, sheet pathologic group
It knits and is embedded using the wax stone of this kind of hole.
Preferably, the step 1. in the forming method of hole of wax stone adopt and be molded with a mold.
Preferably, 1. the forming method use drilling of the hole of middle wax stone or the punching method of forming form the step.
Advantageous effects of the invention:
The present invention relates to a kind of embedding method for particular pathologies tissue, the wax stone with hole is prepared first, when embedding
Pathological tissue is put into hole, then is embedded with wax liquor filling pore, the wax stone hole in this method can limit tissue
It moves and prevents it from toppling over, play the role of fixing organization direction, switch to desired part in slice convenient for user, hole
In the presence of direction when can effectively control tissue block embedding, avoid so also avoiding tweezers institute with tweezers clamping tissue repeatedly
Caused by tissue damage and influence be sliced lesion observation and judgement.
Detailed description of the invention
Fig. 1 is the schematic perspective view that the mold of wax stone is made in embodiment one；
Fig. 2 is the schematic diagram of the section structure of Fig. 1；
Fig. 3 is the schematic perspective view of mold core in Fig. 1；
Fig. 4 is schematic perspective view framed in Fig. 1；
Fig. 5 is the wax stone for using Mold Making described in embodiment one (by taking cylindrical casting as an example)；
Fig. 6 is the state diagram (pushing mold core, take out wax stone) of Mold Making wax stone described in embodiment one；
In figure: 1. framed, 11. convex edges, 12. sliding slots, 13. framed bottoms, 2. mold cores, 21. wax stone grooves, 22. pilot holes,
23. guide rail, 3. bottom plates, 31. screws, 41. casting, 42. top plates, 43. mandrils, 44. springs, 5. wax stones, 51. holes.
Specific embodiment
Embodiment one, a kind of embedding method for particular pathologies tissue, comprising the following steps:
1. production has the wax stone of hole；
2. embedding, the wax stone made is put on platform, the pipe of quasi- embedding-cavity-like tissue or lamellar structure are put into wax stone
In hole, position and angle with tweezers adjustment pathological tissue make the destination region for intending being sliced in pathological tissue close to wax stone
The bottom of hole finally fills wax liquor into the hole of wax stone and carries out organization embedding；
It is sliced 3. can be adhered to the wax stone for being embedded with pathological tissue after intrapore wax liquor solidifies completely on platform wood.
Step 1. in the hole of wax stone the middle part of wax stone is set.
The step 1. in wax stone hole cross sectional shape be circle, pipe-cavity-like tissue use this kind of hole wax
Block is embedded.
Step 1. in the cross sectional shape of hole of wax stone be ellipse or rectangle, lamellar structure is using this kind of hole
Wax stone is embedded.
The step 1. in the forming method of hole of wax stone adopt and be molded with a mold；It can also be using drilling or punching
Molding.
This method is mainly used for the embedding of tiny or relatively thin pathological tissue, can be tiny pipe-cavity-like tissue, such as mouse
Femur, Xenopus laevis bone, zebra fish juvenile fish etc., are also possible to relatively thin pathological tissue, as sheet chicken embryo vitellinae membrana, chick chorioallantoic membrane,
Mesenterium etc..
In order to be more clearly understood and realize embedding method of the invention, applicant provides one kind
The mold for making the above-mentioned wax stone with hole, referring to Fig. 1-6, the wax stone mold includes framed and mold core；
The framed framework for rectangular shape, the mold core and framed matching, and be arranged in framed inside, framed
Bottom is fixed with bottom plate, is equipped with one week convex edge stretched out in horizontal direction, the mold core side in the upper edge of framed medial surface
Equipped with vertical guide rail, vertical and the sliding slot with guide rail, the mold core are equipped on framed medial surface and passes through guide rail
It is matched with framed upper hopper chute, makes the sliding pair that mold core is vertical in framed internal formation；
The upper surface of mold core is equipped with several wax stone grooves, and the center of each wax stone bottom portion of groove is equipped with leading for vertically communicated mold core
Xiang Kong is equipped with matching and vertical perforative mandril in each pilot hole, and the lower end of every mandril is fixed on the upper of bottom plate
Surface, the upper end of every mandril are fixed with horizontal top plate, the bottom Corresponding matching of the corresponding wax stone groove of every piece of top plate,
It is set with spring in the lower part of every mandril, the upper end of the spring is supported on the bottom of mold core, the lower end of spring is supported on bottom
The upper surface of plate, and mold core is supported on the lower surface of top plate by the bottom of wax stone groove, in the upper surface of each top plate
Center is fixed with the casting stretched out straight up, and the upper surface of mold core is stretched out in the upper end of each casting.
The casting is cylindric or straight quadrangular shape, can be selected according to the form flexible in size of quasi- investing tissue
The straight quadrangular shape casting of the casting of correspondingly-shaped, the i.e. cylindrical casting of different-diameter or different-thickness forms casting
The shape of the hole of wax stone matches with the shape of pathological tissue as far as possible, embeds convenient for the orientation of pathological tissue.
The wax stone groove is inverted positive quadrangular frustum pyramid shaped, and the wax stone of the be poured formation of wax stone groove of the shape can
Effectively completely demould.
The bottom plate is fixed by screws in framed bottom surface, disassembly, peace convenient for mold core and its internal other component
Dress and maintenance cleaning.
The lower end of every mandril passes through screw and fixes with bottom plate, according to use demand, can flexibly dismount mandril and top
Plate replaces casting of different shapes in turn.
The described framed, bottom plate, mold core, mandril, top plate and casting are stainless steel material or copper material.
The principle and step of the Mold Making wax stone:
The casting of the form or size selection correspondingly-shaped organized when according to practical embedding is simultaneously mounted on mold, i.e. pipe-cavity-like
Tissue uses columned casting, and lamellar structure uses the casting of straight quadrangular shape；Check that mold guarantees mold core in spring force
Under the action of supported and be adjacent on top plate naturally；Then the paraffin of melting is cast in wax stone groove, be poured stone
The liquid level of wax is not higher than the upper surface of mold core, according to actual needs the quantity of selection casting wax stone groove；Casting terminates and in room
Under temperature after the solidification of wax liquor surface layer, mold is integrally put into the solidification for accelerating paraffin in cold water；Paraffin to be cast solidifies completely
Afterwards, gently press down on mold core with hand, move mold core gradually downward, at the same solidify wax stone relative to wax stone groove gradually by
The top plate ejection of mandril support, makes the wax stone of solidification realize demoulding；Then gently lifted upwards with hand wax stone make itself and casting it
Between completely disengage to get to formulate shape central hole wax stone；
Wax stone morphosis prepared by above-mentioned mold is complete and has hole, by tiny tissue or relatively thin group when use
It knits and is put into hole, then embedded with wax liquor filling, hole can limit moving and preventing it from toppling over for tissue, play fixed group
Knit the effect of direction, convenient for user slice when switch to ideal position, avoid with tweezers repeatedly clamping tissue and caused by
Tissue damage, and then influence the observation and judgement of slice lesion.
The production of wax stone is not limited to the mold in embedding method of the present invention；It is come out with other molds or tool making
Shape and structure is similar with wax stone structure in the present invention, belongs to protection scope of the present invention.
Claims (6)
1. a kind of embedding method for particular pathologies tissue, it is characterized in that: method includes the following steps:
1. production has the wax stone of hole；
2. embedding, the wax stone made is put on platform, the lumen shape of quasi- embedding or sheet pathological tissue are put into wax stone
In hole, position and angle with tweezers adjustment pathological tissue make the destination region for intending being sliced in pathological tissue close to wax stone
The bottom of hole finally fills wax liquor into hole and carries out organization embedding；
3. the wax stone for being embedded with pathological tissue is adhered on platform wood and is sliced after intrapore wax liquor solidifies completely.
2. the embedding method according to claim 1 for particular pathologies tissue, it is characterized in that: the step 1. in
The middle part of wax stone is arranged in wax stone hole.
3. the embedding method according to claim 1 for particular pathologies tissue, it is characterized in that: the step 1. in
The cross sectional shape of wax stone hole is circle, and pipe-cavity-like pathological tissue is embedded using the wax stone of this kind of hole.
4. the embedding method according to claim 1 for particular pathologies tissue, it is characterized in that: the step 1. in
The cross sectional shape of wax stone hole is ellipse or rectangle, and sheet pathological tissue is embedded using the wax stone of this kind of hole.
5. the embedding method according to claim 1 for particular pathologies tissue, it is characterized in that: the step 1. in
The forming method of the hole of wax stone, which is adopted, to be molded with a mold.
6. a kind of embedding method for particular pathologies tissue according to claim 1, it is characterized in that: the step is 1.
The forming method of the hole of middle wax stone is using drilling or the molding of the punching method of forming.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500511.2A CN110132702A (en) | 2019-06-11 | 2019-06-11 | A kind of embedding method for particular pathologies tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500511.2A CN110132702A (en) | 2019-06-11 | 2019-06-11 | A kind of embedding method for particular pathologies tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110132702A true CN110132702A (en) | 2019-08-16 |
Family
ID=67580993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910500511.2A Pending CN110132702A (en) | 2019-06-11 | 2019-06-11 | A kind of embedding method for particular pathologies tissue |
Country Status (1)
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| CN (1) | CN110132702A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113970472A (en) * | 2020-07-23 | 2022-01-25 | 北京市神经外科研究所 | Tissue section method and forming device for tissue section |
| CN114383922A (en) * | 2021-12-29 | 2022-04-22 | 南京金域医学检验所有限公司 | Embedding technology standardized operation method |
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| CN105717082A (en) * | 2016-02-02 | 2016-06-29 | 管晓翔 | Method for detecting heterogeneity in triple-negative breast cancer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113970472A (en) * | 2020-07-23 | 2022-01-25 | 北京市神经外科研究所 | Tissue section method and forming device for tissue section |
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| CN114383922A (en) * | 2021-12-29 | 2022-04-22 | 南京金域医学检验所有限公司 | Embedding technology standardized operation method |
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Application publication date: 20190816 |