CN110118863A - A kind of high-throughput drug automatic splinter screening device for screening and method - Google Patents
A kind of high-throughput drug automatic splinter screening device for screening and method Download PDFInfo
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- CN110118863A CN110118863A CN201811107615.9A CN201811107615A CN110118863A CN 110118863 A CN110118863 A CN 110118863A CN 201811107615 A CN201811107615 A CN 201811107615A CN 110118863 A CN110118863 A CN 110118863A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
Abstract
The present invention provides a kind of high-throughput drug automatic splinter screening device for screening and methods, including three-dimensional moving device, air-channel system and electrostatic field on-off controlling system, three-dimensional moving device includes 4 motor mould groups, spray head and the bottom plate platform that X, Y, Z axis direction is arranged in, bottom plate platform is slidably arranged in y-axis motor mould group upper surface, spray head is slidably arranged in X-axis motor mould group front end face and face bottom plate platform, and three-dimensional moving device is controlled by displacement control system；Spray head is attached with air-channel system；Spray head and bottom plate platform are controlled by electrostatic field on-off controlling system, and displacement control system, air-channel system and electrostatic field on-off controlling system are controlled by upper computer control system.The device realizes automation control, reduces working strength, reduces operating error, improve screening efficiency, high voltage electric field manufacture microlayer model technology reduces dosing, greatly reduces experimental cost, to meet the demand of quick, high efficiency, milligram ammonia drug screening technology.
Description
Technical field
The present invention relates to bio-mechanical fields, more particularly, to a kind of high-throughput drug automatic splinter screening device for screening and method.
Background technique
With the appearance of combinatorial chemistry, drug screening person is faced with more and more new target drones or potential effective component,
High Throughput Screening Assay just comes into being in this context.High Throughput Screening Assay since the 1990s because scientific and technological
Development and the high flux screening based on 96 orifice plates that occurs, it is quick, efficient due to its features such as, be suitble to preliminary screening,
It is based on the experimental method of molecular level and cellular level, using microplate format as experimental tool carrier, with automatic operation
System executes experimentation, and the application of the technology greatly improves the speed of the screening to target molecule, active material and drug
Degree, can obtain a large amount of drug information by once testing, be widely used by international most drugs research institution, and obtain
Quickly development, it has also become one of technical way of drug screening.Therefore the micromation, automation of High Throughput Screening Assay
It is the inexorable trend of future development.
Application No. is the patent documents of CN201520749213.4 to disclose a kind of medicament screening apparatus, is screened using gravity
Machine is screened, i.e., drug is placed on sieve, is realized and is screened by the gravity of the vibration of sieve and drug itself, passed through
Swingle is stirred drug, while the hairbrush on swingle can also automatically clear up sieve, to prevent sieve pore from sending out
Raw blocking.
Application No. is the patent documents of CN200810203864.8 to disclose a kind of miniflow for high-flux medicaments sifting
Cellular array chip, methods and applications are controlled, chip is once valve control channel layer from the top down, is used for various concentration concurrent
Various kinds of cell is acted on, high-flux cell drug screening is carried out.
Existing micro-fluidic chip generallys use the materials such as glass, organic glass, organosilicon, cost of manufacture it is relatively expensive and
And it needs accurate pump driving reagent to flow in runner and is reacted.
Summary of the invention
The purpose of the present invention is to provide a kind of high-throughput drug automatic splinter screening device for screening and methods, and low reagent may be implemented and disappear
Consumption, high-throughput drug screening, stimulation of the parallel parsing different pharmaceutical concentration to different cell quantities and its reality in plate
When observe and detection, provide the completely new device and thinking of one kind for high-flux medicaments sifting and cell drug research.
To achieve the goals above, the present invention the following technical schemes are provided:
A kind of high-throughput drug automatic splinter screening device for screening, including three-dimensional moving device, air-channel system and electrostatic field on-off controlling system,
The three-dimensional moving device includes multiple motor mould groups, spray head and bottom plate platform, and the multiple motor mould group is first motor mould
Group, the second motor mould group, third motor mould group and the 4th motor mould group, the first motor mould group are parallel with the second motor mould group
The Z-direction of three-dimensional coordinate is set, and the third motor mould group is horizontally set on the X-direction of three-dimensional coordinate, its both ends is slided
Dynamic to be arranged in first motor mould group and the second motor mould group, the 4th motor mould group is arranged in first motor mould group and second
In the Y direction of three-dimensional coordinate, the bottom plate platform is slidably arranged in the upper of the 4th motor mould group for bottom surface between motor mould group
Surface, the spray head are slidably arranged in the front end face and face bottom plate platform of third motor mould group, and the three-dimensional moving device is logical
Displacement control system is crossed to be controlled；The spray head is attached with air-channel system；The spray head and bottom plate platform pass through electrostatic
Field on-off controlling system is controlled, and the displacement control system, air-channel system and electrostatic field on-off controlling system pass through upper
Machine control system is controlled.
Further, each motor mould group includes that motor, mould group mounting base, two motor cabinets, sliding block, screw rod and two lead
Bar, two motor cabinets are separately fixed at the both ends of mould group mounting base, and the outer end face of one of motor cabinet has been screwed
Motor is set on the sliding block there are two guide rod hole and a screw hole, and the center of described two motor cabinets is equipped with motor block hole,
The opposite end face of described two motor cabinets is equipped with the mounting hole that matches with two guide rods, and two guide rods penetrate in guide rod hole it
Both ends are fastened in the mounting hole on motor cabinet, and the screw rod penetrates its both ends in screw hole and matches with motor block hole for interference
It closes, one end of the screw rod is attached by shaft coupling and motor, is threadedly engaged between the screw rod and screw hole.
Further, each motor mould group is screwed limit switches on the motor cabinet of motor side, described
Upper computer control system is equipped with three axis and returns to zero mechanism.
Further, the bottom plate platform includes bottom plate, and the bottom plate is fixed on the upper table of the sliding block of the 4th motor mould group
Face, the upper surface of the bottom plate are fixed with the bottom crown of connection positive pole by insulated plastic stock column, and the bottom crown is equipped with
Hold the groove of multi-hole orifice.
Further, the first motor mould group and the second motor mould group pass through mould group mounting base by fastened by screw in bunch
On case, the third motor mould group by mould group mounting base by fastened by screw first motor mould group and the second motor mould group cunning
On block, the 4th motor mould group passes through mould group mounting base by fastened by screw on steel plate bottom plate, and the spray head passes through spray head and connects
Fitting is fixed on the sliding block of third motor mould group.
Further, the spray head includes rubber air pipe, syringe, two Teflon fixed rings, syringe sleeve, nozzle and needle
The inlet end of syringe is arranged in and by rubber seal in syringe in cylinder shell, the rubber air pipe, and the syringe is set in needle
Cylinder sleeve is interior, its both ends is arranged with a Teflon fixed ring, another end cap of the syringe opposite with the rubber air pipe respectively
Equipped with nozzle, the syringe casing is set to the outside of spray head and is fixedly connected by screw with syringe sleeve, and the nozzle is prominent
For the outer edge of syringe sleeve, the cathode of the nozzle connection high voltage power supply.
Further, the air-channel system includes master control borad, air pump, solenoid valve and air gauge, and the air pump passes through rubber
Pipe is connected on solenoid valve, and the solenoid valve is attached with master control borad, and lead to air gauge for the gas outlet of the solenoid valve, described
Barometric outlet port is attached by rubber air pipe and syringe.
A kind of high-throughput drug auto-screening method, the method are completed using device of the present invention, the method
The following steps are included:
(1) cell hydrogel solution is configured, cell hydrogel solution is packed into syringe；
(2) multi-hole orifice is placed in the groove of bottom crown, opens equipment power supply and upper computer control system, passes through serial ports
The good preset serial port of mode adjustment and baud rate, realize upper computer control system and three-dimensional moving device communication link
It connects；
(3) whole device returns to origin, then moves down Z axis sliding block, opens air-channel system using upper computer control system,
The cell solution prepared in syringe is squeezed out with air pressure, the high voltage electric field formed between spray head and bottom crown makes solution at micron
Grade drop controls spray head by three-dimensional moving device and is directed at hole location, can be by the molten of same cell quantity by the preset time
Liquid, which sprays, to be entered in orifice plate, after waiting cells to spray, using air pressure by solution pumpback, can be realized to the full-automatic of 96 orifice plates
Injection；
(4) medicine ordinance is respectively charged into syringe at different concentration gradients, spray head is directed at each hole location, realized entirely certainly
Multi-hole orifice, is then put into incubator and carries out cell culture by dynamic drug addition, and the growth conditions by observing cell are realized
The screening of drug.
The utility model has the advantages that the arrangement achieves meters the present invention provides a kind of high-throughput drug automatic splinter screening device for screening and method
The automation of calculation machine control, reduces the working strength and experimental cost of drug screening, reduces experimental implementation error, Er Qieti
High screening efficiency, has expanded the availability of drug resource, the use of medicament is reduced with high voltage electric field manufacture microlayer model technology
Amount, greatly reduces experimental cost, to meet the demand of quick, high efficiency, milligram ammonia drug screening technology.
The device has played important application value in following several respects simultaneously:
(1) high-throughput sieve is carried out to the compound in Microbial Metabolites Libraries using high orifice plate by High Throughput Screening Assay
Then choosing screens target compound by corresponding detection technique, to be found to have the microorganism guide of different physiological activity
Compound and acquisition have the bacterial strain of a certain special properties；
(2) device can screen the dosage ratio of constituency activity height, small toxicity；
(3) device not only has in terms of drug screening, searching reactive compound greatly excellent in modernization of cmm
Gesture, and also play an important role in terms of the research of chemical composition of Chinese materia medica, important live part and Chinese medicine compound prescription, it improves
The research level of modernization.
Detailed description of the invention
Fig. 1 is the schematic diagram that hydrogel solution of the embodiment of the present invention 2 with breast cancer cell instills in orifice plate.
Fig. 2 is the structural schematic diagram of high-throughput drug automatic splinter screening device for screening of the present invention.
Fig. 3 is the assembling schematic diagram of motor mould group of the present invention.
Fig. 4 is the schematic diagram of air-channel system of the present invention.
Fig. 5 is the assembling schematic diagram of spray head of the present invention.
In figure: 1, motor mould group；101, motor；102, mould group mounting base；103, motor cabinet；104, sliding block；105, screw rod；
106, guide rod；107, mounting hole；108, limit switch；11, first motor mould group；12, the second motor mould group；13, third motor
Mould group；14, the 4th motor mould group；2, spray head；21, rubber air pipe；22, syringe；23, Teflon fixed ring；24, syringe sleeve；
25, nozzle；26, syringe shell；3, bottom plate platform；31, bottom plate；32, insulated plastic stock column；33, bottom crown；34, multi-hole orifice；4,
Bunch case；5, steel plate bottom plate；6, spray head connector.
Specific embodiment
The invention will now be further described with reference to specific embodiments, but examples are merely exemplary, not to this hair
Bright range constitutes any restrictions.It will be understood by those skilled in the art that without departing from the spirit and scope of the invention
Can with the details and forms of the technical scheme of the invention are modified or replaced, but these modification and replacement each fall within it is of the invention
In protection scope.
A kind of high-throughput drug automatic splinter screening device for screening, as shown in Fig. 2, including three-dimensional moving device, air-channel system and electrostatic
Field on-off controlling system, the three-dimensional moving device includes multiple motor mould groups 1, spray head 2 and bottom plate platform 3, the multiple electricity
Machine mould group be first motor mould group 11, the second motor mould group 12, third motor mould group 13 and the 4th motor mould group 14, described first
Motor mould group 11 and the second motor mould group 12 are arranged in parallel in the Z-direction of three-dimensional coordinate, and the third motor mould group 13 is horizontal
The X-direction of three-dimensional coordinate is set, its both ends is slidably arranged in first motor mould group 11 and the second motor mould group 12 on, it is described
The bottom surface between first motor mould group 11 and the second motor mould group 12 is arranged in the Y-axis side of three-dimensional coordinate in 4th motor mould group 14
To the bottom plate platform 3 is slidably arranged in the upper surface of the 4th motor mould group 14, and the spray head 2 is slidably arranged in third motor
The front end face and face bottom plate platform 3 of mould group 13, the three-dimensional moving device is controlled by displacement control system；The spray
First 2 are attached with air-channel system；The spray head 2 and bottom plate platform 3 are controlled by electrostatic field on-off controlling system, described
Displacement control system, air-channel system and electrostatic field on-off controlling system are controlled by upper computer control system, wherein electrostatic
The high voltage power supply of field is controlled in 5KV or so.Wherein two motor mould groups of Z-direction selection allow for the weight of motor mould group,
Guarantee that kinematic accuracy, two motor mould groups can complete load-bearing and movement well.
As shown in figure 3, each motor mould group includes motor 101, mould group mounting base 102, two motor cabinets 103, sliding block
104, screw rod 105 and two guide rods 106, two motor cabinets 103 are separately fixed at the both ends of mould group mounting base 102, one of them
The outer end face of motor cabinet 103 has been screwed motor 101, is set on the sliding block 102 there are two guide rod hole and a screw rod
Hole, the centers of described two motor cabinets 103 are equipped with motor block hole, the opposite end face of described two motor cabinets 103 be equipped with
The mounting hole 107 that two guide rods 106 match, two guide rods 106 penetrate its both ends in guide rod hole and are fastened on the peace on motor cabinet
It filling in hole 107, it is to be interference fitted that the screw rod 105, which penetrates its both ends and motor block hole in screw hole, the one of the screw rod 105
End is attached by shaft coupling with motor 101, is threadedly engaged between the screw rod 105 and screw hole.Electricity used in it
Machine is stepper motor, and screw lead 8mm guarantees the resolution ratio of movement, and motor driver subdivision and adjustment is 16 subdivisions, to guarantee
The resolution ratio of movement is sufficiently small, and guide rod diameter is 5mm, and mould group mounting base 102 can play the role of support, energy to guide rod 106
Enough guarantee that sliding block 104 slides on guide rod 106 under the driving of control system；Precision is improved, can be accurately positioned each solid
Positioning is set；Robust motion is guaranteed, and can significantly improve the stationarity of movement, reduces the vibration of entire platform, while
The effect of noise reduction can be played.
Each motor mould group is screwed limit switches 108 on the motor cabinet of motor side, for realizing fixed
Position control and terminal position limitation protection, once motor movement arrive zero-bit when, limit switch unlatching, stop its movement, can protect in this way
Card mechanism will not be moved then, the possibility of mechanism damage occur；The upper computer control system is equipped with three axis and returns to zero mechanism, can be with
Play the role of checking position before each machine run, improves the accuracy of movement, reduce entire mechanical system
Repetitive error.
The bottom plate platform 3 includes bottom plate 31, and the bottom plate 31 is fixed on the upper surface of the sliding block of the 4th motor mould group 14,
The upper surface of the bottom plate 31 is fixed with the bottom crown 33 of connection positive pole by insulated plastic stock column 32, on the bottom crown 33
Equipped with the groove for holding multi-hole orifice 34, multi-hole orifice 34 and bottom plate 31 are made by acrylic board, are guaranteed insulation, are made
Making high voltage electric field not with insulated plastic stock column 32 influences the movement of entire stepper motor, while guaranteeing safety.
The first motor mould group 11 and the second motor mould group 12 pass through mould group mounting base by fastened by screw in bunch case 4
On, the third motor mould group 13 is by mould group mounting base by fastened by screw in first motor mould group 11 and the second motor mould group 12
Sliding block on, the 4th motor mould group 14 by mould group mounting base by fastened by screw on steel plate bottom plate 5, the spray head 2 is logical
Spray head connector 6 is crossed to be fixed on the sliding block of third motor mould group 13.
As shown in figure 5, the spray head 2 includes rubber air pipe 21, syringe 22, two Teflon fixed rings 23, syringe sleeves
24, the inlet end of syringe 22 is arranged in and by rubber seal in syringe in nozzle 25 and syringe shell 26, the rubber air pipe 21
In 22, the syringe 22 is set in syringe sleeve 24, its both ends is arranged with a Teflon fixed ring 23 respectively, with the rubber
The other end of the opposite syringe 22 of glue tracheae 21 is arranged with nozzle 25, and the syringe shell 26 is sheathed on the outside of spray head 2 and leads to
It crosses screw to be fixedly connected with syringe sleeve 24, the nozzle 25 protrudes from the outer edge of syringe sleeve 24, and the nozzle 25 connects
The cathode of high voltage power supply.
As shown in figure 4, the air-channel system includes master control borad, air pump, solenoid valve and air gauge, the air pump passes through rubber
Pipe is connected on solenoid valve, and the solenoid valve is attached with master control borad, and lead to air gauge for the gas outlet of the solenoid valve, described
Barometric outlet port is attached by rubber air pipe and syringe.
A kind of high-throughput drug auto-screening method, the method are completed using device of the present invention, the method
The following steps are included:
(1) cell hydrogel solution is configured, cell hydrogel solution is packed into syringe；
(2) multi-hole orifice is placed in the groove of bottom crown, opens equipment power supply and upper computer control system, passes through serial ports
The good preset serial port of mode adjustment and baud rate, realize upper computer control system and three-dimensional moving device communication link
It connects；
(3) whole device returns to origin, then moves down Z axis sliding block, opens air-channel system using upper computer control system,
The cell solution prepared in syringe is squeezed out with air pressure, the high voltage electric field formed between spray head and bottom crown makes solution at micron
Grade drop controls spray head by three-dimensional moving device and is directed at hole location, can be by the molten of same cell quantity by the preset time
Liquid, which sprays, to be entered in orifice plate, after waiting cells to spray, using air pressure by solution pumpback, can be realized to the full-automatic of 96 orifice plates
Injection；
(4) medicine ordinance is respectively charged into syringe at different concentration gradients, spray head is directed at each hole location, realized entirely certainly
Multi-hole orifice, is then put into incubator and carries out cell culture by dynamic drug addition, and the growth conditions by observing cell are realized
The screening of drug.
The design principle of device of the present invention are as follows:
The principle of high voltage electric field manufacture microlayer model is the effect of polymeric fluid electrostatic atomization, forms the small jet stream of polymer.And
High voltage electric field manufactures a kind of this special process of microlayer model, and polymer solution carries out jet spinning in strong electrical field, and this mode can
To produce the compound of nanometer grade diameter.In the present apparatus, the setting of entire spray head is also because of high voltage electric field manufacture microlayer model
Conceptual design, wherein superjacent presses down drug solution by constant air pressure, guarantees that the thickness of wire vent is stablized, in this way may be used
To guarantee that the drug concentration squeezed out is guaranteed, due to there is the control of solenoid valve that can arbitrarily control medication amount, to realize low
The consumption of reagent.Air-channel system connects the spray head of the cathode of high voltage power supply, then places in lower section for 96 orifice plates of drug screening
On the pole plate for being connected with high-voltage power cathode, such drug reaches spray head, drug under the extruding of the constant air pressure of adjustable on-off
Solution has arrived at spray head, and under the action of the high voltage electric field formed due to the positive/negative plate by high pressure, the drop at syringe needle can prolong
Polymer filaments at exhibition.
Embodiment 2
The specifically used method of device of the present invention is explained in detail by taking the drug screening of breast cancer cell as an example, Fig. 1
The schematic diagram in orifice plate is instilled for the hydrogel solution with breast cancer cell.
Hydrogel solution with breast cancer cell with Preparation Method it is as follows: the interim cell 5mL of logarithmic growth will be in
PBS cleaning removal phase of cell growth in metabolin, then with 3mL concentration for 0.25% condition of the Trypsin pancreatin at 37 degree
Lower reaction three minutes, allows adherent cell to be in free state, and the culture medium pancreatin that 3mL is added digests the termination of cell, puts
Enter centrifuge tube and be placed in centrifuge to be centrifuged several minutes, cell is allowed to be sunken to below centrifuge tube, takes out centrifuge tube, outwell supernatant, add
Enter to be mixed with the culture medium of hydrogel, then goes out cell quantity using tally meter, band can be allotted by required concentration dilution in this way
The hydrogel solution of cell.
Multi-hole orifice is placed in the groove of bottom crown, equipment power supply and upper computer control system is opened, passes through string
The good preset serial port of mode adjustment and baud rate of mouth realize the communication of upper computer control system and three-dimensional moving device
Connection.Whole device can be first allowed to return to origin before realizing hydrogel irradiation cure test to check, limit at this moment
Switch plays the role of protection mechanism, prevents machine from interfering and damaging.After returning to zero, Z axis sliding block is moved down, is utilized
Upper computer control system opens air-channel system and is squeezed out the cell solution prepared in syringe with air pressure, then between spray head and aluminium sheet
The high voltage electric field of formation can make solution at micron order drop, in this way can be by the molten of same cell quantity by the preset time
Liquid sprays into orifice plate, and after waiting cells to spray, using air pressure by solution pumpback, the full-automatic injecting to 96 orifice plates can be realized
It penetrates, then changes ultraviolet lamp, accurately guarantee that the cell quantity in each hole is almost the same.
Paclitaxel solution is prepared by 20 μM, 10 μM, 5 μM, 2.5 μM, 2 μM, 1 μM, 0.5 μM and 0.2 μM, then by 8
The drug of concentration gradient is placed in syringe, and the syringe equipped with drug solution is changed, and is directed at each hole location using syringe, is realized complete
Automatic drug addition, each concentration gradient make 8 samples, and then orifice plate is put into incubator, culture 24 hours, 48 hours,
72 hours and 96 hours, CCK8 solution is during which added, then hatches a few hours, is put into microplate reader in this way, just according to absorbance
It may determine that the optimal concentration for killing cancer cell, to reduce the complexity of goal in research, be quickly made for screening active ingredients
Sample library.By large batch of disposable dosage ratio, new component formula is formed.It lives for subsequent detection technology to selection
Property high, small toxicity component investigate and lay the groundwork, this for obtain it is significant in efficacy improve, toxic side effect reduces, target spot is clear, matter
Controllable new drug is measured to prepare.
Claims (8)
1. a kind of high-throughput drug automatic splinter screening device for screening, which is characterized in that including three-dimensional moving device, air-channel system and electrostatic field
On-off controlling system, the three-dimensional moving device include multiple motor mould groups, spray head and bottom plate platform, the multiple motor mould group
For first motor mould group, the second motor mould group, third motor mould group and the 4th motor mould group, the first motor mould group and second
Motor mould group is arranged in parallel in the Z-direction of three-dimensional coordinate, and the third motor mould group is horizontally set on the X-axis side of three-dimensional coordinate
It is slidably arranged in first motor mould group and the second motor mould group to, its both ends, the 4th motor mould group setting is in the first electricity
In the Y direction of three-dimensional coordinate, the bottom plate platform is slidably arranged in the 4th for bottom surface between machine mould group and the second motor mould group
The upper surface of motor mould group, the spray head be slidably arranged in third motor mould group front end face and face bottom plate platform, described three
Dimension mobile device is controlled by displacement control system；The spray head is attached with air-channel system；The spray head and bottom plate
Platform is controlled by electrostatic field on-off controlling system, the displacement control system, air-channel system and the control of electrostatic field on-off
System is controlled by upper computer control system.
2. a kind of high-throughput drug automatic splinter screening device for screening according to claim 1, which is characterized in that each motor mould group packet
Motor, mould group mounting base, two motor cabinets, sliding block, screw rod and two guide rods are included, two motor cabinets are separately fixed at the installation of mould group
The both ends of seat, the outer end face of one of motor cabinet has been screwed motor, set on the sliding block there are two guide rod hole and
The center of one screw hole, described two motor cabinets is equipped with motor block hole, and the opposite end face of described two motor cabinets is equipped with
The mounting hole matched with two guide rods, two guide rods penetrate in the mounting hole that its both ends is fastened on motor cabinet in guide rod hole,
It is interference fit that the screw rod, which penetrates its both ends and motor block hole in screw hole, and one end of the screw rod passes through shaft coupling and electricity
Machine is attached, and is threadedly engaged between the screw rod and screw hole.
3. a kind of high-throughput drug automatic splinter screening device for screening according to claim 2, which is characterized in that each motor mould group is leaned on
Limit switches are screwed on the motor cabinet of nearly motor side, the upper computer control system is equipped with three axis and returns to zero mechanism.
4. a kind of high-throughput drug automatic splinter screening device for screening according to claim 2, which is characterized in that the bottom plate platform packet
Bottom plate is included, the bottom plate is fixed on the sliding block upper surface of the 4th motor mould group, and the upper surface of the bottom plate passes through insulated plastic stock column
It is fixed with the bottom crown of connection positive pole, the bottom crown is equipped with the groove for holding multi-hole orifice.
5. a kind of high-throughput drug automatic splinter screening device for screening according to claim 2, which is characterized in that the first motor mould
Group and the second motor mould group pass through mould group mounting base by fastened by screw on bunch case, and the third motor mould group is pacified by mould group
Seat is filled by fastened by screw on the sliding block of first motor mould group and the second motor mould group, the 4th motor mould group is pacified by mould group
Seat is filled by fastened by screw on steel plate bottom plate, the spray head is fixed on the sliding block of third motor mould group by spray head connector.
6. a kind of high-throughput drug automatic splinter screening device for screening according to claim 2, which is characterized in that the spray head includes rubber
Syringe is arranged in glue tracheae, syringe, two Teflon fixed rings, syringe sleeve, nozzle and syringe shell, the rubber air pipe
Inlet end simultaneously passes through rubber seal in syringe, and the syringe is set in syringe sleeve, its both ends is arranged with a spy respectively
Fluorine dragon fixed ring, the other end of the syringe opposite with the rubber air pipe are arranged with nozzle, and the syringe casing is set to spray head
Outside and be fixedly connected by screw with syringe sleeve, the nozzle protrudes from the outer edge of syringe sleeve, and the nozzle connects
Connect the cathode of high voltage power supply.
7. a kind of high-throughput drug automatic splinter screening device for screening according to claim 1, which is characterized in that the air-channel system packet
Master control borad, air pump, solenoid valve and air gauge are included, the air pump is connected on solenoid valve by rubber tube, the solenoid valve and master
Control plate be attached, lead to air gauge for the gas outlet of the solenoid valve, the barometric outlet port by rubber air pipe with
Syringe is attached.
8. a kind of high-throughput drug auto-screening method, which is characterized in that the method uses described in any one of claim 1-7
Device complete, the described method comprises the following steps:
(1) cell hydrogel solution is configured, cell hydrogel solution is packed into syringe；
(2) multi-hole orifice is placed in the groove of bottom crown, opens equipment power supply and upper computer control system, passes through serial ports
The good preset serial port of mode adjustment and baud rate, realize upper computer control system and three-dimensional moving device communication link
It connects；
(3) whole device returns to origin, then moves down Z axis sliding block, opens air-channel system using upper computer control system,
The cell solution prepared in syringe is squeezed out with air pressure, the high voltage electric field formed between spray head and bottom crown makes solution at micron
Grade drop controls spray head by three-dimensional moving device and is directed at hole location, can be by the molten of same cell quantity by the preset time
Liquid, which sprays, to be entered in orifice plate, after waiting cells to spray, using air pressure by solution pumpback, can be realized to the full-automatic of 96 orifice plates
Injection；
(4) medicine ordinance is respectively charged into syringe at different concentration gradients, spray head is directed at each hole location, realized entirely certainly
Multi-hole orifice, is then put into incubator and carries out cell culture by dynamic drug addition, and the growth conditions by observing cell are realized
The screening of drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811107615.9A CN110118863A (en) | 2018-09-21 | 2018-09-21 | A kind of high-throughput drug automatic splinter screening device for screening and method |
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| CN201811107615.9A CN110118863A (en) | 2018-09-21 | 2018-09-21 | A kind of high-throughput drug automatic splinter screening device for screening and method |
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| CN110118863A true CN110118863A (en) | 2019-08-13 |
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| CN201811107615.9A Pending CN110118863A (en) | 2018-09-21 | 2018-09-21 | A kind of high-throughput drug automatic splinter screening device for screening and method |
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| CN114459859A (en) * | 2022-02-16 | 2022-05-10 | 北京卢米斯生物科技有限公司 | High-flux dioxin automatic detection device based on luciferase reporter gene method |
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