CN110118769A - A kind of gold nanoparticle and preparation method thereof for detecting heavy metal ion - Google Patents
A kind of gold nanoparticle and preparation method thereof for detecting heavy metal ion Download PDFInfo
- Publication number
- CN110118769A CN110118769A CN201910410944.9A CN201910410944A CN110118769A CN 110118769 A CN110118769 A CN 110118769A CN 201910410944 A CN201910410944 A CN 201910410944A CN 110118769 A CN110118769 A CN 110118769A
- Authority
- CN
- China
- Prior art keywords
- solution
- ion
- gold nanoparticle
- heavy metal
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 239000010931 gold Substances 0.000 title claims abstract description 67
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 67
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 65
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 150000002500 ions Chemical class 0.000 claims abstract description 94
- 229910001422 barium ion Inorganic materials 0.000 claims abstract description 48
- XDFCIPNJCBUZJN-UHFFFAOYSA-N barium(2+) Chemical compound [Ba+2] XDFCIPNJCBUZJN-UHFFFAOYSA-N 0.000 claims abstract description 47
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 claims abstract description 47
- 238000001514 detection method Methods 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 110
- 239000007864 aqueous solution Substances 0.000 claims description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 claims description 33
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 238000006243 chemical reaction Methods 0.000 claims description 20
- 239000008399 tap water Substances 0.000 claims description 20
- 235000020679 tap water Nutrition 0.000 claims description 20
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 19
- 239000011707 mineral Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 14
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 claims description 12
- PZXYILUXRGTFGD-UHFFFAOYSA-N 2,5,8,11,14,17-hexaoxabicyclo[16.4.0]docosa-1(18),19,21-trien-20-amine Chemical compound O1CCOCCOCCOCCOCCOC2=CC(N)=CC=C21 PZXYILUXRGTFGD-UHFFFAOYSA-N 0.000 claims description 9
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 239000001509 sodium citrate Substances 0.000 claims description 8
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000004847 absorption spectroscopy Methods 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000012086 standard solution Substances 0.000 claims description 4
- 238000002371 ultraviolet--visible spectrum Methods 0.000 claims description 4
- -1 Pb2+ Ion Chemical class 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N N-phenyl amine Natural products NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 2
- WDSPXPVKEVYDEH-UHFFFAOYSA-N ethanol;3-sulfanylpropanoic acid Chemical compound CCO.OC(=O)CCS WDSPXPVKEVYDEH-UHFFFAOYSA-N 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 31
- 238000010521 absorption reaction Methods 0.000 abstract description 22
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000009471 action Effects 0.000 abstract description 4
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 4
- 239000001257 hydrogen Substances 0.000 abstract description 4
- 238000009825 accumulation Methods 0.000 abstract description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract description 3
- 238000005054 agglomeration Methods 0.000 abstract description 2
- 230000002776 aggregation Effects 0.000 abstract description 2
- 230000007246 mechanism Effects 0.000 abstract description 2
- 150000002894 organic compounds Chemical class 0.000 abstract description 2
- 238000004364 calculation method Methods 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- 238000000862 absorption spectrum Methods 0.000 description 6
- 239000011259 mixed solution Substances 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- FAXDZWQIWUSWJH-UHFFFAOYSA-N 3-methoxypropan-1-amine Chemical compound COCCCN FAXDZWQIWUSWJH-UHFFFAOYSA-N 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 238000003968 anodic stripping voltammetry Methods 0.000 description 2
- 229910052788 barium Inorganic materials 0.000 description 2
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 2
- IWOUKMZUPDVPGQ-UHFFFAOYSA-N barium nitrate Chemical compound [Ba+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O IWOUKMZUPDVPGQ-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 159000000009 barium salts Chemical class 0.000 description 1
- CJDPJFRMHVXWPT-UHFFFAOYSA-N barium sulfide Chemical compound [S-2].[Ba+2] CJDPJFRMHVXWPT-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- XIEPJMXMMWZAAV-UHFFFAOYSA-N cadmium nitrate Inorganic materials [Cd+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XIEPJMXMMWZAAV-UHFFFAOYSA-N 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000000705 flame atomic absorption spectrometry Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 231100000171 higher toxicity Toxicity 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000008621 organismal health Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
- G01N21/293—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection with colour charts, graduated scales or turrets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N2021/258—Surface plasmon spectroscopy, e.g. micro- or nanoparticles in suspension
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of gold nanoparticles and preparation method thereof for detecting heavy metal ion, the technical method is based on Agglomeration Mechanism, using on organic compound amino and/or carboxyl be capable of forming strong hydrogen bond action or strong pi-pi accumulation formed by the phenyl ring on organic compound and act on, so as to cause gold nanoparticle reunion, so that solution colour changes, the peak position and absorption intensity for causing gold nanoparticle surface plasma resonance absorbtion peak change, the variation directly occurred using the peak position and intensity of naked eyes and ultraviolet and visible absorption peak, rapidly whether can contain Cd in test sample2+、Ba2+、Pb2+Ion and its concentration are realized to Cd in sample2+、Ba2+、Pb2+The quick detection of ion.The technology has many advantages, such as easy to operate, at low cost, applied widely.
Description
Technical field
The invention belongs to nano materials and ion detection field, and in particular to one kind is for detecting Cd2+、Ba2+、Pb2+With much money
Belong to the gold nanoparticle and preparation method thereof of ion.
Background technique
With the rapidly development of industry and society, the ecological environments such as soil, atmosphere and water body are by heavy metal ion dirt
Seriously threatening for dye, causes the concern of numerous environmentalists.Heavy metal pollution is different from organic matter or biological pollution, pollution
Be difficult to eliminate, and can be accumulated in vivo by the enrichment of nature biotechnology, into human body after can with protein and
Enzyme generates certain effect, inactivates organ, leads to slow poisoning or causes the intracorporal disease of people and nervous system and interior
The disorder of excretory system, therefore, heavy metal pollution constitute serious potential hazard to the health of organism.
Barium is heavy metal, is commonly used to various industrial process, such as be electroplated, glass industry, petroleum industry, emerging pigment industry and
Corrosion inhibitor etc., compound are also used for painting, ceramics and rubber etc..So far, the Ba in water pollution2+Ion is still
It is most common.Ba2+Ionic soil soil and water resource, especially barium salt water soluble compound and acid-soluble compound have higher
Toxicity (for example, barium sulphide).The Ba of water middle and high concentration2+Ion can be accumulated and be turned by plant with soluble status
It moves on to other animals.
In heavy metal, lead ion Pb2+With cadmium ion Cd2+Use it is also very universal, such as baked foods, paint, vapour
Oil, cosmetics etc..Cd2+And Pb2+Highest attention because of its environmental pollution, to the harm that the mankind generate by society.Nowadays,
Lake, river and tap water are considered as providing the natural storage of fresh water for the mankind and the ecosystem.Therefore, it monitors in drinking water
The pollution of heavy metal ion, and quickly detection identification heavy metal ion pollution, and reduction pollution risk becomes to the maximum extent
Inevitable choice.Traditional sensing technology has sensitive, flexible feature such as AAS, ICP-AES and ICP-MS, but complicated, holds high
It is expensive, and need to be not suitable for real-time detection using large-scale instrument and equipment.In order to solve these problems, people successfully develop electricity
Chemical detection method, part heavy metal ion for identification, for example, anodic stripping voltammetry (ASV), have it is highly sensitive,
The features such as easy to operate and inexpensive is a kind of for detecting the strong tools of heavy metal ion.But ASV on-site test needs
Sensor-based system needs a small amount of miniature instrument equipment that could complete effectively to detect.
So far, detection of heavy metal ion method has been rapidly developed, but is different its detection of detection architecture
Method is different, and the technology applied is in difference, includes flame atomic absorption spectrometry, fibre optical sensor and electrochemistry side
Method.The Kanokwan Chaichana of Kasetsart university, Thailand develops part heavy metal analysis method, detection method tool
There are the features such as high sensitivity, but its sample preparation complexity and time and effort consuming etc..In recent years, the hair that nanotechnology is advanced by leaps and bounds
Exhibition makes it also have system in terms of detection of heavy metal ion and in-depth study, and obtains certain achievement.The present invention passes through synthesis
A kind of 6 derivative of -18 crown- of amino benzo, then modifies gold nanoparticle with the derivative and 3- mercaptopropionic acid jointly, using repairing
Gold nanoparticle and Cd after decorations2+、Ba2+、Pb2+The specific function of heavy metal ion causes the color of gold nanoparticle and ultraviolet
The variation of absorption spectrum is realized to Cd2+、Ba2+、Pb2+The quick detection of heavy metal ion.
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficienciess of the prior art, provide a kind of stability it is good, operation
Step is simple, can quickly, accurately detect Cd in aqueous solution2+、Ba2+、Pb2+The method of heavy metal ion.
The present invention solves the technical solution that above-mentioned technical problem is taken are as follows: a kind of for detecting the Jenner of heavy metal ion
The preparation method of rice corpuscles, using sodium citrate, 4'- amino-Benzo-18-crown-6 and 3- mercaptopropionic acid respectively as reduction
The gold of trivalent, is reduced into the gold of zeroth order by agent, dressing agent and stabilizer, and stable, evengranular gold nanoparticle is made
(Fig. 1)；The aqueous solution of prepared gold nanoparticle is added separately to containing certain density Cd2+、Ba2+、Pb2+Heavy metal ion
Solution in, solution colour becomes grey or grey black from red.4'- amino-Benzo-18-crown-6 and 3- mercaptopropionic acid are made
It is acted on for dressing agent and gold nanoparticle, gold nanoparticle has rich and varied hydrogen bond action, passes through its amino-NH2Or carboxylic
Base-COOH forms strong hydrogen bond action or forms strong pi-pi accumulation effect by phenyl ring, reunites so as to cause gold nanoparticle,
So that solution colour changes, realize to Cd in solution2+、Ba2+、Pb2+The naked eye of heavy metal ion quickly detects；Pass through gold
The reunion of nanoparticle causes the peak position of gold nanoparticle surface plasma resonance absorbtion peak and intensity to change, realization pair
Cd in solution2+、Ba2+、Pb2+The quantitative detection of heavy metal ion.
The structure of 4'- amino-Benzo-18-crown-6 and 3- mercaptopropionic acid is as shown below:
4'- amino-Benzo-18-crown-6 structure diagram and 3- mercaptopropionic acid structure diagram
A kind of preparation method of gold nanoparticle provided by the invention, specifically comprises the following steps:
Certain density aqueous solution of chloraurate is added in round-bottomed flask, is heated with stirring to boiling, rapidly joins citric acid
The aqueous solution of sodium reacts 5~15 minutes, and the second of 4'- amino-Benzo-18-crown-6 DMF solution and 3- mercaptopropionic acid is added
Alcoholic solution obtains reaction mixture solution, and the pH value of reaction mixture solution is adjusted to 10~13 with HCl, and solution colour is by light yellow
Become red, standing is cooled to room temperature, and gold nanoparticle is made, is put into 0~4 DEG C of refrigerator and saves backup；
The concentration of the aqueous solution of chloraurate is 3~6mM；The mass fraction of the sodium citrate aqueous solution is 1%；
The concentration of the DMF solution of the 4'- amino-Benzo-18-crown-6 is 0.1mM；
The concentration of the ethanol solution of the 3- mercaptopropionic acid is 0.15mM；
The DMF is N,N-dimethylformamide；The substance for participating in reaction is that analysis is pure.
The present invention also provides the purposes of the gold nanoparticle, which is characterized in that the gold nanoparticle is used for
Detect Cd2+、Ba2+、Pb2+Heavy metal ion specifically comprises the following steps:
By the aqueous solution of prepared gold nanoparticle respectively with various concentration Cd2+、Ba2+、Pb2+The mark of heavy metal ion
Quasi- solution reaction is made standard card (Fig. 2), the card including blank assay result according to different color changes, for not
With the on-site test of sample to be tested under environment；Or
By the aqueous solution of prepared gold nanoparticle and various concentration Cd2+、Ba2+、Pb2+The standard of heavy metal ion is molten
Then liquid reaction passes through ultraviolet-visible absorption spectroscopy, the gold nanoparticle surface plasma resonance absorbtion peak after test reaction
The variation that peak position and intensity occur, draws ultraviolet-visible absorption spectroscopy and standard curve (Fig. 3), for be checked under varying environment
Test result is substituted into standard curve by the detection of sample, is compared, is calculated, and is judged in sample to be tested with the presence or absence of Cd2+、
Ba2+、Pb2+Ion calculates Cd in sample to be tested2+、Ba2+、Pb2+The concentration of ion, to realize to Cd in sample2+、Ba2+、
Pb2+The quantitative detection of heavy metal ion；
Open hole detection is limited to Cd2+For 10nM, Ba2+For 20nM, Pb2+For 50nM；
Uv-vis spectra detection is limited to Cd2+For 5nM, Ba2+For 10nM, Pb2+For 20nM；
The sample to be tested can be mineral water, tap water or lake water.
In conclusion the present invention provides a kind of gold nanoparticles and preparation method thereof, and with prepared gold nano
Particle is for detecting Cd2+、Ba2+、Pb2+The technology of heavy metal ion；The technical method is based on Agglomeration Mechanism, has using dressing agent
Amino or carboxyl on machine compound are capable of forming strong specific hydrogen bond action or are formed by the phenyl ring on organic compound
Strong pi-pi accumulation effect, so that solution colour changes, causes gold nanoparticle table so as to cause gold nanoparticle reunion
The peak position and absorption intensity at face plasma resonance absorption peak change, and therefore, are directly divided using naked eyes and UV, visible light
Luminosity determined, rapidly whether can contain Cd in test sample2+、Ba2+、Pb2+The content of heavy metal ion or ion,
It realizes to Cd in sample2+、Ba2+、Pb2+The quick detection of heavy metal ion.
Compared with the prior art, the advantages of the present invention are as follows: the present invention mention a kind of gold nanoparticle and preparation method thereof with
The application technology of different kinds of ions detection, the technical method is simple to operate, raw material is cheap and easy to get, at low cost, detection is quick, spirit
Sensitivity is high, selectivity is strong and implementable live naked eye colorimetric detection, is suitable for mineral water, tap water, lake water and other are multiple
Cd in miscellaneous system2+、Ba2+、Pb2+Quick detection, have extensive potential using value.
Detailed description of the invention
The TEM figure of Fig. 1, gold nanoparticle of the present invention, particle diameter range 10-20nm；
The standard card of Fig. 2, different detected components of the present invention, the card including blank assay result；Wherein, scheme
2a is Cd2+The standard card of concentration (μM), Fig. 2 b are Ba2+The standard card of (μM) concentration, Fig. 2 c are Pb2+The mark of (μM) concentration
Quasi- card；
The ultraviolet-visible absorption spectroscopy and standard curve of Fig. 3, different detected components of the present invention, standard curve is institute
The linear relationship between the ultravioletvisible absorption volume efficiency and concentration of metal ions of detection metal ion is stated, ordinate is test
The ratio of the corresponding absorption intensity of two maximum absorption wavelengths of component, abscissa are the concentration of corresponding metal ion；
The particle surface plasma resonance ultraviolet-visible absorption spectroscopy of Fig. 4, different component, abc is 4'- amino-benzene in figure
And -18- crown ether -6,3-MPA is 3- mercaptopropionic acid, AuNPs is gold nanoparticle.
Specific embodiment
The present invention is further described below by specific embodiment, but does not limit the contents of the present invention.
The production of standard card
The chlorauric acid solution of 5mL5mM is added in the deionized water of 91mL by step (1), and while stirring, while be heated to
Under conditions of boiling, it is 1% sodium citrate solution as reducing agent that 4mL mass fraction, which is added, and after reacting 15min, lemon is made
The aqueous solution of the gold nanoparticle of the reduction of lemon acid group and modification；
Step (2) configures 10 μM of 3-MPA-abc solution: the 3- mercaptopropionic acid (being abbreviated as 3-MPA) of 0.1mmol is dissolved in
In the ethyl alcohol of 90mL, heating stirring, dissolution；Then 4'- amino-phendioxin 8- crown ether-of 0.1mmol is added in the above solution
6 (being abbreviated as abc) and DMF solvent 10mL, heating stirring, reaction 30min, add ethyl alcohol, make the volume 100mL of solution, obtain
Obtain mixture solution；Said mixture solution 1.0mL is taken, the ethyl alcohol of 99mL is added to be diluted, it is molten to obtain final 3-MPA-abc
The concentration of liquid is 10 μM；
A series of aqueous solution of concentration of cadmium ions is prepared with deionized water and cadmium nitrate, final concentration is followed successively by 0 μM, 0.01 μ
M, 0.02 μM, 0.05 μM, 0.1 μM, 0.3 μM, 1 μM, 5 μM, 7 μM and 8 μM of standard solution, takes the standard of various concentration molten respectively
Each 0.1mL of liquid, and the aqueous solution of gold nanoparticle prepared by 0.9mL step (1) is respectively added, and system in step (2) is added
Standby 10 μ L of 3-MPA-abc solution obtains mixed solution, stands 20 minutes, different variations is presented in the color of mixed solution, obtains
Cd2+The standard card (Fig. 2 a) of ion concentration, the card including blank assay result.
A series of aqueous solution of barium ions concentration is prepared with deionized water and barium nitrate, final concentration is followed successively by 0 μM, 0.02 μ
M, 0.05 μM, 0.08 μM, 0.5 μM, 1 μM, 5 μM, 8 μM, 10 μM and 15 μM of standard solution, takes the standard of various concentration molten respectively
Each 0.1mL of liquid, and the aqueous solution of gold nanoparticle prepared by 0.9mL step (1) is respectively added, and system in step (2) is added
Standby 10 μ L of 3-MPA-abc solution obtains mixed solution, stands 20 minutes, different variations is presented in the color of mixed solution, obtains
Ba2+The standard card (Fig. 2 b) of ion concentration, the card including blank assay result.
A series of aqueous solution of plumbum ion concentrations is prepared with deionized water and plumbi nitras, final concentration is followed successively by 0 μM, 0.05 μ
M, 0.1 μM, 0.3 μM, 0.5 μM, 0.8 μM, 1 μM, 3 μM, 5 μM and 6 μM of standard solution, takes the standard of various concentration molten respectively
Each 0.1mL of liquid, and the aqueous solution of gold nanoparticle prepared by 0.9mL step (1) is respectively added, and system in step (2) is added
Standby 10 μ L of 3-MPA-abc solution obtains mixed solution, stands 20 minutes, different variations is presented in the color of mixed solution, obtains
Pb2+The standard card (Fig. 2 c) of ion concentration, the card including blank assay result.
Draw standard curve:
Uv-visible absorption spectra measurement is carried out to the above-mentioned standard sample being prepared, in the section 400~800nm
The variation of the ultraviolet-ray visible absorbing intensity measured, and record the absorbance ratio at 525nm and 685nm, 695nm, 690nm.With
The concentration of standard sample is made standard curve (Fig. 3) as abscissa, absorbance ratio as ordinate；By linear fit,
Obtain Cd2+The linear equation of solion standard curve is y=0.9937 X+0.1588, R2=0.9917；Ba2+Solion
The linear equation of standard curve is y=0.0524 X+0.0289, R2=0.9984；Pb2+The linear side of solion standard curve
Journey is y=0.0823 X+0.0213, R2=0.9934.
Using standard card and standard curve to various concentration Cd2+、Ba2+、Pb2+Heavy metal ion is detected, naked eye inspection
Survey limit: Cd2+For 10nM, Ba2+For 20nM, Pb2+For 50nM；Uv-vis spectra detection limit: Cd2+For 5nM, Ba2+For 10nM, Pb2 +For 20nM；The calculation formula of uv-vis spectra detection limit is K × SD/S, and K=3, SD are standard deviation, and S is slope.
Embodiment 1
The aqueous solution of chloraurate of 10mL6mM concentration is added in round-bottomed flask, is heated with stirring to boiling, rapidly joins 20mL
4'- amino-phendioxin 8- crown ether-of 30mL0.1mM is added after reaction 15 minutes in the aqueous solution of 1% sodium citrate of mass fraction
The ethanol solution of the 3- mercaptopropionic acid of 6 DMF solution and 30mL0.15mM, obtains reaction mixture solution, reaction mixture solution
PH value be regulated to 10 with HCl, solution colour becomes red from light yellow, and standing is cooled to room temperature, and gold nanoparticle is made.It will
Gold nanoparticle is made and carries out TEM test, pattern is as shown in Figure 1.
Embodiment 2
The aqueous solution of chloraurate of 20mL3mM is added in round-bottomed flask, is heated with stirring to boiling, rapidly joins 20mL mass
After five minutes, 4'- amino-Benzo-18-crown-6 of 20mL0.1mM is added in the aqueous solution of 1% sodium citrate of score, reaction
The ethanol solution of DMF solution and 30mL0.15mM3- mercaptopropionic acid, obtains reaction mixture solution, and regulating and controlling its pH value with HCl is 13,
Solution colour becomes red from light yellow, and standing is cooled to room temperature, and gold nanoparticle is made.
Embodiment 3
The aqueous solution of chloraurate of 20mL5mM concentration is added in round-bottomed flask, is heated with stirring to boiling, rapidly joins 30mL
After ten minutes, 4'- amino-phendioxin 8- hat of 40mL 0.1mM is added in the aqueous solution of 1% sodium citrate of mass fraction, reaction
The DMF solution of ether -6 and the ethanol solution of 40mL0.15mM3- mercaptopropionic acid, the pH value of reaction mixture solution are regulated to HCl
12, solution colour becomes red from light yellow, and standing is cooled to room temperature, and gold nanoparticle is made.
Embodiment 4
Cd in " mineral water "2+、Ba2+、Pb2+The detection of ion
(a) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Cd2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " mineral water " is separately added into
Into two parts of gold nanoparticle aqueous solutions, mixed liquor A and mixed liquid B are formed；After reaction 15 minutes, mixed liquid B is compared
With the color of mixed liquor A, if color change, there are Cd in " mineral water " solution to be detected2+Ion, color becomes if it does not exist
Change, then Cd is not present in " mineral water " solution to be detected2+Ion or comparing calculation mixed liquid B and the ultraviolet of mixed liquor A can
See absorption intensity and peak value, if its absorption intensity and peak value change, there are Cd in " mineral water " solution to be detected2+From
Son, if Cd is not present in " mineral water " solution to be detected there is no variation2+Ion.
(b) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Ba2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " mineral water " is separately added into
Into two parts of gold nanoparticle aqueous solutions, mixed liquor C and mixed liquor D is formed；After reaction 15 minutes, mixed liquor D is compared
With the color of mixed liquor C, if color change, there are Ba in " mineral water " solution to be detected2+Ion, color becomes if it does not exist
Change, then Ba is not present in " mineral water " solution to be detected2+Ion or comparing calculation mixed liquor D and the ultraviolet of mixed liquor C can
See absorption intensity and peak value, if its absorption intensity and peak value change, there are Ba in " mineral water " solution to be detected2+From
Son, if Ba is not present in " mineral water " solution to be detected there is no variation2+Ion.
(c)) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Pb2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " mineral water " is separately added into
Into the aqueous solution of two parts of gold nanoparticles, mixed liquor E and mixed liquor F is formed；After reaction 15 minutes, mixed liquor is compared
The color of F and mixed liquor E, if color change, there are Pb in " mineral water " solution to be detected2+Ion, if it does not exist color
Change, then Pb is not present in " mineral water " solution to be detected2+Ion or comparing calculation mixed liquor F and mixed liquor E's is ultraviolet
Visible absorbance intensity and peak value, if its absorption intensity and peak value change, there are Pb in " mineral water " solution to be detected2+
Ion, if Pb is not present in " mineral water " solution to be detected there is no variation2+Ion.
Embodiment 5
Cd in " tap water "2+、Ba2+、Pb2+The detection of ion
(a) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Cd2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " tap water " is separately added into
Into the aqueous solution of two parts of gold nanoparticles, mixed liquor A and mixed liquid B are formed；After reaction 15 minutes, mixed liquor is compared
The color of B and mixed liquor A, if color change, there are Cd in " tap water " solution to be detected2+Ion, if it does not exist color
Change, then Cd is not present in " tap water " solution to be detected2+Ion or comparing calculation mixed liquid B and mixed liquor A it is ultraviolet
Visible absorbance intensity and peak value, if its absorption intensity and peak value change, there are Cd in " tap water " solution to be detected2+
Ion, if Cd is not present in " tap water " solution to be detected there is no variation2+Ion.
(b) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Ba2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " tap water " is separately added into
Into the aqueous solution of two parts of gold nanoparticles, mixed liquor C and mixed liquor D is formed；After reaction 15 minutes, mixed liquor is compared
The color of D and mixed liquor C, if color change, there are Ba in " tap water " solution to be detected2+Ion, if it does not exist color
Change, then Ba is not present in " tap water " solution to be detected2+Ion or comparing calculation mixed liquor D and mixed liquor C's is ultraviolet
Visible absorbance intensity and peak value, if its absorption intensity and peak value change, there are Ba in " tap water " solution to be detected2+
Ion, if Ba is not present in " tap water " solution to be detected there is no variation2+Ion.
(c)) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Pb2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " tap water " is separately added into
Into the aqueous solution of two parts of gold nanoparticles, mixed liquor E and mixed liquor F is formed；After reaction 15 minutes, mixed liquor is compared
The color of F and mixed liquor E, if color change, there are Pb in " tap water " solution to be detected2+Ion, if it does not exist color
Change, then Pb is not present in " tap water " solution to be detected2+Ion or comparing calculation mixed liquor F and mixed liquor E's is ultraviolet
Visible absorbance intensity and peak value, if its absorption intensity and peak value change, there are Pb in " tap water " solution to be detected2+
Ion, if Pb is not present in " tap water " solution to be detected there is no variation2+Ion.
Embodiment 6
Cd in " lake water "2+、Ba2+、Pb2+The detection of ion
(a) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Cd2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " lake water " is added separately to
In the aqueous solution of two parts of gold nanoparticles, mixed liquor A and mixed liquid B are formed；After reaction 15 minutes, mixed liquid B is compared
With the color of mixed liquor A, if color change, there are Cd in " lake water " solution to be detected2+Ion, color becomes if it does not exist
Change, then Cd is not present in " lake water " solution to be detected2+The UV, visible light of ion or comparing calculation mixed liquid B and mixed liquor A
Absorption intensity and peak value, if its absorption intensity and peak value change, there are Cd in " lake water " solution to be detected2+Ion,
If Cd is not present in " lake water " solution to be detected there is no variation2+Ion.
(b) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Ba2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " lake water " is added separately to
In the aqueous solution of two parts of gold nanoparticles, mixed liquor C and mixed liquor D is formed；After reaction 15 minutes, mixed liquor D is compared
With the color of mixed liquor C, if color change, there are Ba in " lake water " solution to be detected2+Ion, color becomes if it does not exist
Change, then Ba is not present in " lake water " solution to be detected2+The UV, visible light of ion or comparing calculation mixed liquor D and mixed liquor C
Absorption intensity and peak value, if its absorption intensity and peak value change, there are Ba in " lake water " solution to be detected2+Ion,
If Ba is not present in " lake water " solution to be detected there is no variation2+Ion.
(c)) aqueous solution of two parts of isometric identical prepared gold nanoparticles is taken；It prepares and is free of Pb2+Ion (is being examined
Survey in range) aqueous solution solution as a comparison, the isometric solution to be detected of comparative solution and " lake water " is added separately to
In the aqueous solution of two parts of gold nanoparticles, mixed liquor E and mixed liquor F is formed；After reaction 15 minutes, mixed liquor F is compared
With the color of mixed liquor E, if color change, there are Pb in " lake water " solution to be detected2+Ion, color becomes if it does not exist
Change, then Pb is not present in " lake water " solution to be detected2+The UV, visible light of ion or comparing calculation mixed liquor F and mixed liquor E
Absorption intensity and peak value, if its absorption intensity and peak value change, there are Pb in " lake water " solution to be detected2+Ion,
If Pb is not present in " lake water " solution to be detected there is no variation2+Ion.
Embodiment 7
Identify and distinguishes Cd2+、Ba2+、Pb2+Ion
The aqueous solution of the gold nanoparticle of 0.9mL, be separately added into different sample cells the 0.1mM cadmium of 0.1mL, barium,
The aqueous solution of plumbum ion concentration after mixing, adds 10uL10 μM of 3-MPA-abc solution, reacts 15min, carry out it is ultraviolet-can
See that absorption spectrum is tested, as a result as shown in figure 4, different fractions tested ultravioletvisible absorption intensity, peak value it is different and they
Color change different distinguish Cd, it can be achieved that identifying2+、Ba2+、Pb2+Heavy metal ion.
Embodiment 8
Using uv-visible absorption spectra instrument, measurement contains Cd2+The A of the sample to be tested of ion695nm/A525nmRatio is 0.6,
Substitute into Cd2+The linear equation y=0.9937 X+0.1588 of solion standard curve, can calculate X=0.4440 to get
Cd2+The concentration of ion is 0.4440 μM；
Embodiment 9
Using uv-visible absorption spectra instrument, measurement contains Ba2+The A of the sample to be tested of ion685nm/A525nmRatio is 0.6,
Substitute into Ba2+The linear equation y=0.0524 X+0.0289 of solion standard curve, can calculate X=10.90 to get
Ba2+The concentration of ion is 10.90 μM；
Embodiment 10
Using uv-visible absorption spectra instrument, measurement contains Pb2+The A of the sample to be tested of ion690nm/A525nmRatio is 0.6,
Substitute into Pb2+The linear equation y=0.0823 X+0.0213 of solion standard curve, can calculate X=7.032 to get
Pb2+The concentration of ion is 7.032 μM；
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
It for specific embodiments of the present invention, is not intended to restrict the invention, all any modifications made in spirit of the invention
With improve etc., should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of for detecting the preparation method of the gold nanoparticle of heavy metal ion, which is characterized in that utilize 4'- amino-benzene
And -18- crown ether -6 and 3- mercaptopropionic acid prepare gold nanoparticle as dressing agent, specifically includes the following steps:
Certain density aqueous solution of chloraurate is added in round-bottomed flask, is heated with stirring to boiling, rapidly joins certain density
The DMF of certain density dressing agent 4'- amino-Benzo-18-crown-6 is added after reacting 5~15min in sodium citrate aqueous solution
The ethanol solution of solution and suitable 3- mercaptopropionic acid obtains reaction mixture solution, the pH value HCl tune of reaction mixture solution
Control is 10~13, and solution colour becomes red from light yellow, and standing is cooled to room temperature, and gold nanoparticle is made, is put into 0~4 DEG C
Refrigerator in save backup；
The concentration of the aqueous solution of chloraurate is 3~6mM；
The mass fraction of the sodium citrate aqueous solution is 1%；
The concentration of the DMF solution of the 4'- amino-Benzo-18-crown-6 is 0.1mM；
The concentration of the 3- mercaptopropionic acid ethanol solution is 0.15mM；
The DMF is N,N-dimethylformamide；
The substance for participating in reaction is that analysis is pure.
2. a kind of purposes of gold nanoparticle made from method as described in claim 1, which is characterized in that the gold nanoparticle
For detecting Cd2+、Ba2+、Pb2+Heavy metal ion specifically comprises the following steps:
By the aqueous solution of prepared gold nanoparticle respectively with various concentration Cd2+、Ba2+、Pb2+The standard of heavy metal ion is molten
Liquid reaction, is made standard card according to different color changes, the card including blank assay result, under varying environment to
The on-site test of test sample；Or；
By the aqueous solution of prepared gold nanoparticle and various concentration Cd2+、Ba2+、Pb2+The standard solution of heavy metal ion is anti-
It answers, then passes through ultraviolet-visible absorption spectroscopy, the peak position of the gold nanoparticle surface plasma resonance absorbtion peak after test reaction
And the variation that intensity occurs, ultraviolet-visible absorption spectroscopy and standard curve are drawn, for sample to be tested under varying environment
Detection, substitutes into standard curve for test result, is compared, calculates, and judges in sample to be tested with the presence or absence of Cd2+、Ba2+、Pb2+
Ion calculates Cd in sample to be tested2+、Ba2+、Pb2+The concentration of ion, to realize to Cd in sample2+、Ba2+、Pb2+Heavy metal
The quantitative detection of ion；
Open hole detection is limited to Cd2+For 10nM, Ba2+For 20nM, Pb2+For 50nM；
Uv-vis spectra detection is limited to Cd2+For 5nM, Ba2+For 10nM, Pb2+For 20nM；
The sample to be tested can be mineral water, tap water or lake water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910410944.9A CN110118769B (en) | 2019-05-16 | 2019-05-16 | Gold nanoparticles for detecting heavy metal ions and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910410944.9A CN110118769B (en) | 2019-05-16 | 2019-05-16 | Gold nanoparticles for detecting heavy metal ions and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110118769A true CN110118769A (en) | 2019-08-13 |
| CN110118769B CN110118769B (en) | 2021-12-21 |
Family
ID=67522676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910410944.9A Active CN110118769B (en) | 2019-05-16 | 2019-05-16 | Gold nanoparticles for detecting heavy metal ions and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110118769B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110554023A (en) * | 2019-09-26 | 2019-12-10 | 辽宁大学 | surface-enhanced Raman spectroscopy substrate for specifically detecting Ni 2+ and preparation method and application thereof |
| CN111751310A (en) * | 2020-06-30 | 2020-10-09 | 华北科技学院 | Gold nanoparticles with glycopolypeptide, preparation method and application |
| CN113049573A (en) * | 2019-12-27 | 2021-06-29 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Rubidium or cesium ion detection reagent combination, kit and detection method |
| US20210402472A1 (en) * | 2018-11-21 | 2021-12-30 | University Of Leeds | Nanomaterials |
| CN114965456A (en) * | 2022-05-30 | 2022-08-30 | 泉州市水务水质检测有限公司 | Water quality detection method |
| CN115178746A (en) * | 2022-07-13 | 2022-10-14 | 宁波大学 | Preparation method and application of gold nanoparticles for detecting mercury ions in cosmetic water |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445259A (en) * | 2015-11-10 | 2016-03-30 | 宁波大学 | Method for quickly detecting clenbuterol based on functionalized gold nanoparticles |
| CN105954245A (en) * | 2016-05-02 | 2016-09-21 | 福建医科大学 | BSA/3-MPA-gold nanocluster-based NOR logic gate and construction method thereof |
| CN106290280A (en) * | 2016-08-19 | 2017-01-04 | 宁波大学 | The method quickly detecting tripolycyanamide based on the Y type DNA structure connecting quantum dot and gold colloidal |
| CN108672715A (en) * | 2018-04-25 | 2018-10-19 | 安徽师范大学 | Functional modification gold nanoparticle and preparation method thereof, the detection method of metal mercury ions and its application |
| CN108941601A (en) * | 2018-07-25 | 2018-12-07 | 宁波大学 | A kind of gold nanoparticle and preparation method thereof |
-
2019
- 2019-05-16 CN CN201910410944.9A patent/CN110118769B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445259A (en) * | 2015-11-10 | 2016-03-30 | 宁波大学 | Method for quickly detecting clenbuterol based on functionalized gold nanoparticles |
| CN105954245A (en) * | 2016-05-02 | 2016-09-21 | 福建医科大学 | BSA/3-MPA-gold nanocluster-based NOR logic gate and construction method thereof |
| CN106290280A (en) * | 2016-08-19 | 2017-01-04 | 宁波大学 | The method quickly detecting tripolycyanamide based on the Y type DNA structure connecting quantum dot and gold colloidal |
| CN108672715A (en) * | 2018-04-25 | 2018-10-19 | 安徽师范大学 | Functional modification gold nanoparticle and preparation method thereof, the detection method of metal mercury ions and its application |
| CN108941601A (en) * | 2018-07-25 | 2018-12-07 | 宁波大学 | A kind of gold nanoparticle and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| HSIN-YUN CHANG ET AL: "Using Rhodamine 6G-Modified Gold Nanoparticles To Detect Organic Mercury Species in Highly Saline Solutions", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
| VAIBHAVKUMAR N.MEHTA ET AL: "selective visual detection of Pb（II） ion via gold nanoparticles coated with dithiocarbamate-modified 4’-aminobenzo-18-crown-6", 《MICROCHIM ACTA》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210402472A1 (en) * | 2018-11-21 | 2021-12-30 | University Of Leeds | Nanomaterials |
| CN110554023A (en) * | 2019-09-26 | 2019-12-10 | 辽宁大学 | surface-enhanced Raman spectroscopy substrate for specifically detecting Ni 2+ and preparation method and application thereof |
| CN110554023B (en) * | 2019-09-26 | 2022-08-30 | 辽宁大学 | Specific detection Ni 2+ Surface enhanced Raman spectroscopy substrate and preparation method and application thereof |
| CN113049573A (en) * | 2019-12-27 | 2021-06-29 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Rubidium or cesium ion detection reagent combination, kit and detection method |
| CN113049573B (en) * | 2019-12-27 | 2022-08-02 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Rubidium or cesium ion detection reagent combination, kit and detection method |
| CN111751310A (en) * | 2020-06-30 | 2020-10-09 | 华北科技学院 | Gold nanoparticles with glycopolypeptide, preparation method and application |
| CN111751310B (en) * | 2020-06-30 | 2023-02-17 | 华北科技学院 | Gold nanoparticles with glycopolypeptide, preparation method and application |
| CN114965456A (en) * | 2022-05-30 | 2022-08-30 | 泉州市水务水质检测有限公司 | Water quality detection method |
| CN115178746A (en) * | 2022-07-13 | 2022-10-14 | 宁波大学 | Preparation method and application of gold nanoparticles for detecting mercury ions in cosmetic water |
| CN115178746B (en) * | 2022-07-13 | 2024-03-01 | 宁波大学 | Preparation method and application of gold nanoparticles for detecting mercury ions in cosmetic water |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110118769B (en) | 2021-12-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110118769A (en) | A kind of gold nanoparticle and preparation method thereof for detecting heavy metal ion | |
| Bojdi et al. | Synthesis, characterization and application of novel lead imprinted polymer nanoparticles as a high selective electrochemical sensor for ultra-trace determination of lead ions in complex matrixes | |
| Li et al. | Detection of trace nickel ions with a colorimetric sensor based on indicator displacement mechanism | |
| Choudhary et al. | Green biomimetic silver nanoparticles as invigorated colorimetric probe for Hg2+ ions: A cleaner approach towards recognition of heavy metal ions in aqueous media | |
| Tan et al. | Selective detection of nanomolar Cr (VI) in aqueous solution based on 1, 4-dithiothreitol functionalized gold nanoparticles | |
| Yi et al. | Smartphone-based ratiometric fluorescent definable system for phosphate by merged metal− organic frameworks | |
| Li et al. | Gold nanoparticles based colorimetric probe for Cr (III) and Cr (VI) detection | |
| He et al. | A low-cost and label-free assay for hydrazine using MnO2 nanosheets as colorimetric probes | |
| CN107021953A (en) | A kind of coumarin fluorescent probe and preparation method and its application on detection hypochlorite ion | |
| Rajamanikandan et al. | β-Cyclodextrin protected gold nanoparticle based cotton swabs as an effective candidate for specific sensing of trace levels of cyanide | |
| CN103048287A (en) | Sulfur ion measurement method based on gold nanoparticles as simulated peroxidase | |
| Zeng et al. | Novel N, F co-doped carbon dots to detect sulfide and cadmium ions with high selectivity and sensitivity based on a “turn-off-on” mechanism | |
| Luo et al. | Preparation of nitrogen-doped carbon quantum dots and its application as a fluorescent probe for Cr (vi) ion detection | |
| Radwan et al. | Decorated nanosphere mesoporous silica chemosensors for rapid screening and removal of toxic cadmium ions in well water samples | |
| Khattab et al. | Green metallochromic cellulose dipstick for Fe (III) using chitosan nanoparticles and cyanidin-based natural anthocyanins red-cabbage extract | |
| CN106442448B (en) | A kind of method of quick detection sulphion | |
| Reyes R et al. | Fluorescent molecular sensor for the detection of cadmium in basil roots | |
| Yang et al. | Portable intelligent paper-based sensors for rapid colorimetric and smartphone-assisted analysis of hydrogen peroxide for food, environmental and medical detection applications | |
| Chen et al. | N-doped carbon dots as the multifunctional fluorescent probe for mercury ion, glutathione and pH detection | |
| CN106290319A (en) | A kind of method of silver ion in chemiluminescence detection water sample | |
| CN103217416B (en) | Detection composition, method and kit for detection of bivalent mercury ions | |
| CN103487430A (en) | Trivalent aluminum ion detection reagent and method | |
| CN102768191A (en) | Method for easily detecting trace thallium in water | |
| Sasikumar et al. | Facile preparation of dihydrolipoic acid-stabilized red-emitting silver nanoclusters as a sensitive fluorometric probe for sulfide ions detection | |
| He et al. | Preparation of biomass water‐soluble carbon quantum dots and their application in Cr (VI) ions detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220630 Address after: 214000 building 4, No. 29, Huibei Road, Liangxi District, Wuxi City, Jiangsu Province Patentee after: WUXI ZODOLABS BIOTECH Co.,Ltd. Address before: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee before: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Effective date of registration: 20220630 Address after: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee after: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Address before: 315211, Fenghua Road, Jiangbei District, Zhejiang, Ningbo 818 Patentee before: Ningbo University |
|
| TR01 | Transfer of patent right | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20190813 Assignee: Huzhou Feiying Financial Leasing Co.,Ltd. Assignor: WUXI ZODOLABS BIOTECH Co.,Ltd. Contract record no.: X2023980043088 Denomination of invention: A gold nanoparticle and its preparation method for detecting heavy metal ions Granted publication date: 20211221 License type: Exclusive License Record date: 20231008 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A gold nanoparticle and its preparation method for detecting heavy metal ions Effective date of registration: 20231019 Granted publication date: 20211221 Pledgee: Huzhou Feiying Financial Leasing Co.,Ltd. Pledgor: WUXI ZODOLABS BIOTECH Co.,Ltd. Registration number: Y2023980061901 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |