CN110108682A - The application of several atypia feux rouges silicide dyes - Google Patents
The application of several atypia feux rouges silicide dyes Download PDFInfo
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- CN110108682A CN110108682A CN201910379371.8A CN201910379371A CN110108682A CN 110108682 A CN110108682 A CN 110108682A CN 201910379371 A CN201910379371 A CN 201910379371A CN 110108682 A CN110108682 A CN 110108682A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses the applications of several atypia feux rouges silicide dyes, belong to technical field of analytical chemistry.Several dyestuffs of the present invention can emit the weak red fluorescence that lone pair electrons are assembled on oxygen atom (peak value is about 570nm).It can be used for carrying out image checking in vitro living cells, be with a wide range of applications.And fluorescent dye does not need to obtain by chemical synthesis, and raw material is cheap and easy to get, and it is at low cost, it is easy to spread.
Description
Technical field
The present invention relates to the exploitations and its application of a kind of atypia red dye for cell imaging, belong to analytical chemistry
Technical field.
Background technique
Small molecule organo-silicon compound on silicon atom with functionalizing group are known as functionalized silane, are divided into π type function
SiClx alkane and heteroatom type functionalized silane.Silicon atom has empty 3d track, and it is many unique to impart organo-silicon compound
Property.Wherein, heteroatom type functional group can provide delocalized electrons and lone pair electrons respectively.Therefore, functionalized silane has
There are many performances such as novel structure and better light, electricity, magnetic.On the other hand, compared with traditional carbon compound, function SiClx
Alkane itself has many advantages, such as that synthetic method is simple, thermal stability is high, and optical purity is high.Therefore, functionalized silane and complex exist
The fields such as organic photoelectrical material, chemical sensor and biosensor are widely used.
Fluorescence imaging analysis method has that high sensitivity, selectivity is good, the response time is short, simple operation and other advantages, and right
Cell does not damage substantially, is widely used for the detection or cell fluorescence imaging of various ions and biological species.For cell
The related dye of imaging is also rapidly developed, and some of them have been commercialized.But report at present and be commercialized
Dyestuff be all based on greatly Representative fluorescence group conjugated type molecule.Red dye and application based on atypia fluorescence do not appear in the newspapers
Road.Therefore the new red dye based on atypia fluorescence is developed to have great importance.
Summary of the invention
The purpose of the present invention is to provide the applications of several atypia fluorescent dyes.
The invention adopts the following technical scheme:
The application of several siliceous red dyes of atypia is used for isolated cells image checking；Several atypia are siliceous
Shown in the chemical structural formula of red dye such as formula (I):
Several dyestuffs of the present invention can emit the weak red fluorescence (peak value that lone pair electrons are assembled on oxygen atom
About 570nm).The fluorescence property of several silicide dyes of the present invention is tested.Specific measuring method are as follows: room temperature item
Under part, the PBS/ ethanol solution of 100 μM of dyestuffs is prepared, measures solution fluorescence intensity.See Fig. 1-6.
Several fluorescent dyes of the invention can be used for carrying out image checking in vitro living cells, have a wide range of applications
Prospect.
The invention has the benefit that
Atypia fluorescent dye of the present invention does not need to obtain by chemical synthesis, and raw material is cheap and easy to get, at low cost,
It is easy to spread.
Dyestuff of the present invention issues atypia feux rouges, and this dyestuff has not been reported, has broad application prospects.
In terms of dyestuff of the present invention can be successfully applied to cell imaging.
Detailed description of the invention
Fig. 1 is fluorescence spectrum of the dyestuff AS-1 in PBS/EtOH, λEx=520nm.
Fig. 2 is fluorescence spectrum of the dyestuff AS-2 in PBS/EtOH, λEx=520nm.
Fig. 3 is fluorescence spectrum of the dyestuff AS-3 in PBS/EtOH, λEx=520nm.
Fig. 4 is fluorescence spectrum of the dyestuff AS-4 in PBS/EtOH, λEx=520nm.
Fig. 5 is fluorescence spectrum of the dyestuff AS-5 in PBS/EtOH, λEx=520nm.
Fig. 6 is fluorescence spectrum of the dyestuff AS-6 in PBS/EtOH, λEx=520nm.
Fig. 7 is imaging results of the dyestuff AS-1 in HeLa cell.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention will be further described, but the present invention protects content to be not limited only to this.
Embodiment 1
Fluorescence spectrum of the dyestuff of the present invention under the excitation of 520nm excitation wavelength
It prepares the PBS/EtOH mixed solution of 6 parts of 3mL in advance, contains 20% ethyl alcohol, pH=7.4.It is added respectively into system
30 μM of dye mother solution (mother liquid concentration 10mM) then carries out fluorescence detection (λEx=520nm).Fluorescence spectrum is obtained, sees Fig. 1-
6。
Embodiment 2
Imaging applications of the dyestuff AS-2 in living cells
Hela cell is placed in culture medium (DMEM culture solution and 10% fetal calf serum), the condition that is placed in is 37 DEG C, 5%
CO2And 20%O2Incubator in cultivate 24-48h.(10 μM) of dyestuff AS-2 injections of the present invention are drawn with microsyringe
In culture medium containing Hela cell, 30min is cultivated in continuation in the incubator.It is rinsed later with PBS (phosphate buffer solution)
Culture cell 3 times, then carries out fluorescence imaging.As a result see Fig. 7.
Claims (1)
1. the application of several siliceous red dyes of atypia, which is characterized in that be used for isolated cells image checking；It is described several non-
Shown in the chemical structural formula such as formula (I) of typical siliceous red dye:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910379371.8A CN110108682B (en) | 2019-05-08 | 2019-05-08 | Application of several atypical red silicon-containing dyes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910379371.8A CN110108682B (en) | 2019-05-08 | 2019-05-08 | Application of several atypical red silicon-containing dyes |
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| Publication Number | Publication Date |
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| CN110108682A true CN110108682A (en) | 2019-08-09 |
| CN110108682B CN110108682B (en) | 2021-09-24 |
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| CN201910379371.8A Expired - Fee Related CN110108682B (en) | 2019-05-08 | 2019-05-08 | Application of several atypical red silicon-containing dyes |
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| CN110108682B (en) | 2021-09-24 |
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