CN110089650A - A kind of method of capsicum leaf extract preparation enzyme beverage - Google Patents

A kind of method of capsicum leaf extract preparation enzyme beverage Download PDF

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Publication number
CN110089650A
CN110089650A CN201910417987.XA CN201910417987A CN110089650A CN 110089650 A CN110089650 A CN 110089650A CN 201910417987 A CN201910417987 A CN 201910417987A CN 110089650 A CN110089650 A CN 110089650A
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capsicum leaf
ferment
capsicum
leaf extract
preparation
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邱卫华
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Institute of Process Engineering of CAS
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • A23L2/74Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25Honey; Honey substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/31Leuconostoc
    • A23V2400/325Pseudomesenteroides

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Water Supply & Treatment (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

Low side and devalued is presented in the various product of existing capsicum leaf exploitation, limits the development of capsicum leaf industrial chain.Of the invention overcomes existing capsicum leaf to utilize theory and technical shortcoming, there is provided a kind of method of capsicum leaf extract preparation enzyme beverage, technical solution includes the preparation of capsicum leaf packet, the preparation of capsicum leaf extract, the preparation of ferment microbial inoculum inoculum, the fermentation of capsicum leaf extract ferment, the preparation of capsicum leaf extract enzyme beverage.Using technical solution of the present invention, the jerky taste of capsicum leaf can be not only removed, but also can produce a large amount of beneficial metabolic products and special bioactive substance.Thus, the effect of present invention can be to give full play to active constituent in capsicum leaf and exploitation high added value new product provide new way.

Description

A kind of method of capsicum leaf extract preparation enzyme beverage
[technical field]
The invention belongs to food and food processing fields, and in particular to a kind of side of capsicum leaf extract preparation enzyme beverage Method.
[background technique]
Capsicum belongs to plant kingdom's Angiospermae, Dicotyledoneae, eggplant mesh, Solanaceae, capsicum in classification biologically Belong to, capsicum kind, is a kind of plant of dual-purpose of drug and food.The total output of China capsicum has occupied the first place in the world, and annual output is up to 2 800 ten thousand Ton, about the 46% of world's yield of hot pepper, while being increased every year with 9% speed, bring economic benefit occupies vegetables work First of object.But the utilization rate of the byproduct capsicum leaf as pepper planting is very low, has a large amount of capsicum leaf white every year White discarding causes the great wasting of resources.
Demand with people to green product is increasing, capsicum leaf as green natural product by vast consumption The favor of person and the concern of scientific research personnel.And research shows that a kind of capsicum leaf also plant of dual-purpose of drug and food, and its nutrition Ingredient is better than pepper fruit.Total starch content is higher than 46.95% in capsicum leaf, and total dietary fiber is close to 40%, protein Content may be up to 21.07%, be that the three times of pepper fruit are more, and the content of the microelements such as calcium, iron, manganese, zinc is also high in capsicum leaf In capsicum.In addition Flavonoid substances also rich in, fat, carbohydrate, V in capsicum leafCDeng.Capsicum leaf also has simultaneously There are good nutrition health-care functions.More acid compounds contained by capsicum leaf have anti-oxidant, antiallergy and antitumor etc. a variety of Activity;Contained Flavonoid substances are a kind of very strong antioxidants, can effectively remove intracorporal oxygen radical, prevent cell It degenerates, aging;Capsicum leaf has good inhibiting effect to glucuroide, is the good next of the natural antidiabetic medicine of exploitation Source;It is also very strong to the adsorption capacity of cholesterol.It can be seen that capsicum leaf is to integrate edible, medicinal and health care Natural Food Product.Capsicum leaf is as new vegetables in external extremely popular, the land productivities such as Southeast Asia, Hong Kong of Japan and China, Macao in recent years Nutritional health food is developed with capsicum leaf.Capsicum leaf comprehensive utilization also becomes the hot spot of many scholar's researchs at present.Currently, China The products such as salt marsh capsicum leaf, capsicum leaf foods packed in carton containers, capsicum tealeaves, capsicum leaf beverage are developed.
Food plant ferment is with one or more fresh vegetables, fruit, mushroom, Chinese herbal medicine etc. for raw material, is had through a variety of Beneficial bacteria fermentation and produce, the nutritional ingredients such as vitamin, enzyme, minerals and secondary metabolite rich in functionality it is micro- Biofermentation products.The effect of plant enzyme includes adjusting gastrointestinal function, improves immune function, improves nutrient utilization Rate reduces allergy, reduces the disease incidence of cancer, so ferment to human health is guaranteed, prevents disease and plays a significant role. As people's health care consciousness enhances, ferment is had received widespread attention, and the market demand of ferment is very big, and production technology develops Rapidly.Ferment is very popular at home and abroad at present.Ferment type also becomes diversification.
But the research Yet-have in terms of capsicum leaf ferment product development is more short of.Although there are also pass through microorganism The research of fermented capsicum leaf, as patent 201711405275.3 promotes chilli leaves tea by lactic acid bacteria and saccharomycetes to make fermentation capsicum leaf Mouthfeel;Patent 201410749185.6 ferments to capsicum leaf using golden flower bacterium, can be made into novel health-care tea;Patent 201610373683.4,201711269524.0 etc. using lactic acid bacteria, yeast etc. come pickled hot pepper leaf.But it there is no capsicum leaf ferment Plain beverage related patents.
Fermented tea (Kombucha, tea mushroom tea fungus) is also commonly called as " Hypon " " stomach is precious " etc., generally Refer to a kind of fermentation, slightly alcohol, slight effervesce, sugaring tea beverage.It is with a long history in China, can chase after earliest It traces back to B.C. 220 years or so the Qin Dynasty, quilt is it is believed that be the jewellery that can detoxify and assign people's energy.More and more researchs Show in fermented tea tunning containing including gluconic acid, acetic acid, glucose, fructose, protein, amino acid, vitamin, Microelement, tea polyphenols, caffeine isoreactivity ingredient, thus it is used as functional beverage.And fermented tea to a variety of diseases all With potential therapeutic effect, including anti-oxidant, carcinogenesis, anti-diabetic improves immunity, enhances liver detoxification ability, has Help the treatment etc. of gastric ulcer and high cholesterol.There are reports at present is had solely using the exploitation of fermented tea fermenting plant raw material The beverage products of special flavor and function.But it there is no and studied by the fermented tea of raw material of capsicum leaf.
Thus, the present invention provides a kind of methods of capsicum leaf extract preparation enzyme beverage, to by the battalion of capsicum leaf It supports healthcare function to combine with ferment, develops the capsicum leaf enzyme beverage with peculiar flavour and healthcare function.
[summary of the invention]
Although being prepared into chilli leaves tea about capsicum leaf at present to carry out brewing the technology drunk, simply by punching Bubble utilizes the active constituent in capsicum leaf with being obviously unable to fully, and causes the waste of capsicum leaf resource.On the other hand, existing needle To the various product of capsicum leaf exploitation also all more low side and low value, the development of capsicum leaf industrial chain is limited.Mesh of the invention Be to overcome existing capsicum leaf using theory and technical shortcoming, a kind of capsicum leaf extract is provided and prepares ferment drink The method of material, the effect of to give full play to active constituent in capsicum leaf and exploitation high added value new product provides new way.
Technical scheme is as follows:
A kind of method of capsicum leaf extract preparation enzyme beverage of the present invention, it is preparation including capsicum leaf packet, peppery The preparation of green pepper leaf extract, the preparation of ferment microbial inoculum inoculum, the fermentation of capsicum leaf extract ferment, capsicum leaf extract ferment drink The preparation of material and etc., specifically includes the following steps:
1) preparation of capsicum leaf packet: choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go with sterile water gently Rinse the dust for removing leaf of bamboo surface, room temperature hang airing surface moisture.Capsicum fresh leaf is spread dry to water content 55- 65%;Then it wraps to obtain capsicum leaf packet with 200 mesh gauzes;
2) preparation of capsicum leaf leaching liquor: step (1) obtained according to w/v 1:2-50 (w/v, g/ml) peppery Green pepper leaf packet is put into boiling distilled water after extraction 20min-2h, is taken out capsicum leaf packet, is obtained clear capsicum leaf leaching liquor;
3) tealeaves the preparation of ferment microbial inoculum inoculum: is added 90 according to w/v 1:50-200 (w/v, g/ml) 20min is extracted in DEG C -100 DEG C of hot water, filters to take millet paste;Millet paste pours into sterilized container, according still further to w/v 5% Table sugar is added in~20% (wt%), is inoculated with ferment bacterium agent after stirring sufficiently, at 28-30 DEG C, 180rpm shaken cultivation obtains ferment Plain microbial inoculum inoculum;
4) capsicum leaf extract ferment ferment: step (2) preparation capsicum leaf leaching liquor according to w/v 5%~ Table sugar is added in 20% (wt%), and after mixing evenly, obtained ferment microbial inoculum inoculum, then seals in inoculation step (3) In 28-35 DEG C of standing for fermentation 7-14d;
5) preparation of capsicum leaf extract enzyme beverage: the tunning of filtration step (4) takes the ferment of clear to send out Capsicum leaf extract enzyme beverage is made by allotment, reduced pressure or spray drying etc. in zymotic fluid.
Ferment microbial inoculum above-mentioned is using fermented tea as core microbial inoculum, and individually or compounding lactic acid bacteria, saccharomycete are as capsicum The microbial inoculum of leaf extract enzyme beverage production.Further lactic acid bacteria is lactobacillus plantarum, leuconostoc pseudomesenteroides, acidophilus cream bar One or more of bacterium, the saccharomycete be one of saccharomyces cerevisiae, Hansenula anomala and Bayer Zygosaccharomyces or It is a variety of.
The inoculation volume of ferment microbial inoculum inoculum above-mentioned is the 20%~50% of capsicum leaf extracting liquid volume.
Table sugar above-mentioned includes one or more of white granulated sugar, rock sugar, brown sugar, soft white sugar, honey, concentrated sugarcane juice Mixture.
Table sugar addition concentration above-mentioned refers to the total concentration of several sugar, and when adding more than one sugar, every kind sugared Additive amount is identical.
Tealeaves above-mentioned includes one of green tea, black tea, dark green tea.
Capsicum leaf extract ferment fermentation liquid above-mentioned, is: mentioning in capsicum leaf by the method that enzyme beverage is made in allotment It takes and 10% soft white sugar, 5% honey, 0.2% pectin is added in liquid ferment fermentation liquid, stood more than for 24 hours after mixing evenly, then To pour into clear glass bottle after the filtering of 0.2-0.45um film, capsicum leaf extract enzyme beverage is obtained.
Capsicum leaf concentration ferment drink is made by reduced pressure in 40-50 DEG C in capsicum leaf extract ferment fermentation liquid above-mentioned Material.It is spray-dried again and can be made into capsicum leaf extract ferment powder.
[advantages of the present invention]
The present invention has the advantage that
1) capsicum leaf enzyme beverage production method provided by the invention is using fermented tea as core microbial inoculum, by compounding lactic acid Bacterium and saccharomycete are as ferment microbial inoculum co-fermentation capsicum leaf extract.Fermented tea and is contained based on acetic acid bacteria in the microbial inoculum There is the symbiosis flora of a small amount of saccharomycete and lactic acid bacteria.According to mixing ferment microbial inoculum provided by the present invention, compounding cream can be passed through Sour bacterium and saccharomycete strengthen the interaction between flora, can not only remove the jerky taste of capsicum leaf, increase small molecule Fragrance and nutritional ingredient, to reach optimal flavor;And may advantageously facilitate a large amount of generations of beneficial metabolic product, so that Prepared capsicum leaf extract enzyme beverage has special bioactivity.Finally, the organic acid that fermentation process generates makes Enzyme liquid can be to avoid the pollution of other miscellaneous bacterias.
2) capsicum leaf enzyme beverage production method provided by the invention have the characteristics that simple process, completely it is pollution-free, be The active constituent and exploitation high added value new product for keeping capsicum leaf to greatest extent provide new approach.
[specific implementation method]
The present invention will be further described combined with specific embodiments below, but not exhaustive, those skilled in the art without The simple copy and improvement for crossing creative work belong to the range of the claims in the present invention protection.
Embodiment 1
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go to gently to be rinsed removing leaf of bamboo surface with sterile water Dust, room temperature hang airing surface moisture.Capsicum fresh leaf is spread into drying to water content 55%, is then wrapped up in 200 mesh gauzes Tightly obtain capsicum leaf packet.Capsicum leaf packet is put into boiling distilled water according to w/v 1:5 and extracts 40min, takes out capsicum leaf Packet, obtains clear capsicum leaf leaching liquor.
Green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:100 and extracts 20min, filters to take millet paste;Tea Soup pours into sterilized container, and white granulated sugar is added according still further to w/v 15%, inoculation mixing ferment bacterium after stirring sufficiently Agent: black tea mother bacterial liquid, 20272 (Lactobacillus plantarum of lactobacillus plantarum plant subspecies CICC Subsp.Plantarum, similarly hereinafter), saccharomyces cerevisiae CICC 1251 (Saccharomyces cerevisiae, similarly hereinafter), Bayer Zygosaccharomyces CICC 32677 (Zygosaccharomyces bailii, similarly hereinafter), then at 28 DEG C, 180rpm shaken cultivation 5- 7d obtains ferment microbial inoculum inoculum.
The white granulated sugar and honey of equivalent is added in capsicum leaf leaching liquor according to w/v 10% (wt%), stirring is equal After even, it is inoculated with ferment microbial inoculum inoculum according to the 20% of capsicum leaf extracting liquid volume, is then sealed in 28 DEG C of standing for fermentation 7- 14d;Then the ferment fermentation liquid for filtering to take clear is added 10% soft white sugar, 5% honey, 0.2% pectin, stirs evenly After stand more than for 24 hours, then with 0.2-0.45um film filtering after pour into clear glass bottle, obtain capsicum leaf extract ferment drink Material.
Embodiment 2
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go to gently to be rinsed removing leaf of bamboo surface with sterile water Dust, room temperature hang airing surface moisture.Capsicum fresh leaf is spread into drying to water content 60%, is then wrapped up in 200 mesh gauzes Tightly obtain capsicum leaf packet;Capsicum leaf packet is put into boiling distilled water according to w/v 1:10 and extracts 30min, takes out capsicum leaf Packet, obtains clear capsicum leaf leaching liquor.
Black tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:50 and extracts 20min, filters to take millet paste;Millet paste It pours into sterilized container, the brown sugar and sugar-cane juice of equivalent is added according still further to w/v 20%, inoculation after stirring sufficiently Mix ferment microbial inoculum: black tea mother bacterial liquid, 21115 (Leuconostoc of leuconostoc pseudomesenteroides CICC Pseudomesenteroides), saccharomyces cerevisiae, (the Hansenula anomala of Hansenula anomala CICC 1312 Var.anomala, similarly hereinafter), then at 28 DEG C, 180rpm shaken cultivation 5-7d obtains ferment microbial inoculum inoculum.
The brown sugar and concentrated sugarcane juice of equivalent is added in capsicum leaf leaching liquor according to w/v 20% (wt%), stirs After mixing uniformly, it is inoculated with ferment microbial inoculum inoculum according to the 30% of capsicum leaf extracting liquid volume, is then sealed in 35 DEG C of standing for fermentation 7-14d;The ferment fermentation liquid for filtering to take clear, is then added 10% soft white sugar, 5% honey, 0.2% pectin, and stirring is equal It is stood after even more than for 24 hours, then to pour into clear glass bottle after the filtering of 0.2-0.45um film, obtains capsicum leaf extract ferment drink Material.
Embodiment 3
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go to gently to be rinsed removing leaf of bamboo surface with sterile water Dust, room temperature hang airing surface moisture.Capsicum fresh leaf is spread into drying to water content 65%, is then wrapped up in 200 mesh gauzes Tightly obtain capsicum leaf packet;Capsicum leaf packet is put into boiling distilled water according to w/v 1:2 and extracts 2h, takes out capsicum leaf packet, Obtain clear capsicum leaf leaching liquor.
Black tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:150 and extracts 20min, filters to take millet paste;Tea Soup pours into sterilized container, and the white granulated sugar and rock sugar of equivalent are added according still further to w/v 5%, inoculation after stirring sufficiently Ferment microbial inoculum: black tea mother bacterial liquid, lactobacillus plantarum plant subspecies, saccharomyces cerevisiae, Hansenula anomala is mixed, then at 30 DEG C, 180rpm shaken cultivation 5-7d obtains ferment microbial inoculum inoculum.
The white granulated sugar and rock sugar of equivalent is added in capsicum leaf leaching liquor according to w/v 20% (wt%), stirring is equal After even, it is inoculated with ferment microbial inoculum inoculum according to the 25% of capsicum leaf extracting liquid volume, is then sealed in 30 DEG C of standing for fermentation 7- 14d;The ferment fermentation liquid of clear is filtered to take, capsicum leaf extract ferment then is made by reduced pressure at 40-50 DEG C Beverage.
Embodiment 4
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go to gently to be rinsed removing leaf of bamboo surface with sterile water Dust, room temperature hang airing surface moisture.Capsicum fresh leaf is spread into drying to water content 60%, is then wrapped up in 200 mesh gauzes Tightly obtain capsicum leaf packet;Capsicum leaf packet is put into boiling distilled water according to w/v 1:50 and extracts 30min, takes out capsicum leaf Packet, obtains clear capsicum leaf leaching liquor.
Dark green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:200 and extracts 20min, filters to take millet paste;Tea Soup pours into sterilized container, and white granulated sugar, rock sugar, honey and the concentration sugarcane of equivalent are added according still further to w/v 20% Juice, inoculation mixing ferment microbial inoculum after stirring sufficiently: black tea mother bacterial liquid, 6090 (Lactobacillus of lactobacillus acidophilus CICC Acidophilus, similarly hereinafter), Hansenula anomala, Bayer Zygosaccharomyces, then at 30 DEG C, 180rpm shaken cultivation 5-7d is obtained Ferment microbial inoculum inoculum.
Be added in capsicum leaf leaching liquor according to w/v 20% (wt%) white granulated sugar of equivalent, rock sugar, honey and Concentrated sugarcane juice is inoculated with ferment microbial inoculum inoculum according to the 30% of capsicum leaf extracting liquid volume, is then sealed in after mixing evenly 30 DEG C of standing for fermentation 7-14d;The ferment fermentation liquid for filtering to take clear, be then added 10% soft white sugar, 5% honey, 0.2% pectin is stood after mixing evenly more than for 24 hours, then to pour into clear glass bottle after the filtering of 0.2-0.45um film, is obtained Capsicum leaf extract enzyme beverage.
Embodiment 5
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go to gently to be rinsed removing leaf of bamboo surface with sterile water Dust, room temperature hang airing surface moisture.Capsicum fresh leaf is spread into drying to water content 55%, is then wrapped up in 200 mesh gauzes Tightly obtain capsicum leaf packet;Capsicum leaf packet is put into boiling distilled water according to w/v 1:5 and extracts 40min, takes out capsicum leaf Packet, obtains clear capsicum leaf leaching liquor.
Green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:100 and extracts 20min, filters to take millet paste;Tea Soup pours into sterilized container, and soft white sugar, the concentrated sugarcane juice of equivalent are added according still further to w/v 15%, and stirring is abundant Inoculation mixes ferment microbial inoculum: black tea mother bacterial liquid, lactobacillus acidophilus, lactobacillus plantarum plant subspecies, Hansenula anomala, Bayer afterwards Zygosaccharomyces, then at 32 DEG C, 180rpm shaken cultivation 5-7d obtains ferment microbial inoculum inoculum.
Soft white sugar, the concentrated sugarcane juice of equivalent is added in capsicum leaf leaching liquor according to w/v 20% (wt%), After mixing evenly, it is inoculated with ferment microbial inoculum inoculum according to the 30% of capsicum leaf extracting liquid volume, is then sealed in 35 DEG C of standing hairs Ferment 7-14d;Then 10% soft white sugar, 5% honey, 0.2% pectin is added in the ferment fermentation liquid for filtering to take clear, stirring It is stood after uniformly more than for 24 hours, then to pour into clear glass bottle after the filtering of 0.2-0.45um film, obtains capsicum leaf extract ferment Beverage.

Claims (9)

1. a kind of method of capsicum leaf extract preparation enzyme beverage of the present invention, it is characterised in that including capsicum leaf packet Preparation, the preparation of capsicum leaf extract, the preparation of ferment microbial inoculum inoculum, the fermentation of capsicum leaf extract ferment, capsicum leaf are extracted The preparation of liquid enzyme beverage and etc., specifically use following steps:
(1) preparation of capsicum leaf packet: choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, go gently to be rushed with sterile water Remove the dust for going to leaf of bamboo surface, room temperature hang airing surface moisture.Capsicum fresh leaf is spread dry to water content 55-65%; Then it wraps to obtain capsicum leaf packet with 200 mesh gauzes;
(2) preparation of capsicum leaf leaching liquor: the capsicum leaf for obtaining step (1) according to w/v 1:2-50 (w/v, g/ml) Packet is put into boiling distilled water after extraction 20min-2h, is taken out capsicum leaf packet, is obtained clear capsicum leaf leaching liquor;
(3) preparation of ferment microbial inoculum inoculum: according to w/v 1:50-200 (w/v, g/ml) by tealeaves be added 90 DEG C- 20min is extracted in 100 DEG C of hot water, filters to take millet paste;Millet paste pours into sterilized container, according still further to w/v 5%~ Table sugar is added in 20% (wt%), is inoculated with ferment bacterium agent after stirring sufficiently, at 28-30 DEG C, 180rpm shaken cultivation obtains ferment Microbial inoculum inoculum;
(4) capsicum leaf extract ferment ferment: step (2) preparation capsicum leaf leaching liquor according to w/v 5%~ Table sugar is added in 20% (wt%), and after mixing evenly, obtained ferment microbial inoculum inoculum, then seals in inoculation step (3) In 28-35 DEG C of standing for fermentation 7-14d;
(5) preparation of capsicum leaf extract enzyme beverage: the tunning of filtration step (4) takes the ferment of clear to ferment Capsicum leaf extract enzyme beverage is made by allotment, reduced pressure or spray drying etc. in liquid.
2. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Ferment microbial inoculum is using fermented tea as core microbial inoculum, and individually or compounding lactic acid bacteria, saccharomycete are drunk as capsicum leaf extract ferment Expect the microbial inoculum of production.
3. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that step (4) The inoculation volume of the ferment microbial inoculum inoculum is the 20%~50% of capsicum leaf extracting liquid volume.
4. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Table sugar includes the mixture of one or more of white granulated sugar, rock sugar, brown sugar, soft white sugar, honey, concentrated sugarcane juice.
5. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Tealeaves includes one of green tea, black tea, dark green tea.
6. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Capsicum leaf extract ferment fermentation liquid is by the method that enzyme beverage is made in allotment: in capsicum leaf extract ferment fermentation liquid 10% soft white sugar of middle addition, 5% honey, 0.2% pectin, stand more than for 24 hours, after mixing evenly then with 0.2-0.45um film mistake Clear glass bottle is poured into after filter, obtains capsicum leaf extract enzyme beverage.
7. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Capsicum leaf extract enzyme beverage is made by reduced pressure in 40-50 DEG C in capsicum leaf extract ferment fermentation liquid.
8. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 1, it is characterised in that described Capsicum leaf extract ferment fermentation liquid after being concentrated under reduced pressure, then is spray-dried and capsicum leaf extract ferment is made in 40-50 DEG C Powder.
9. a kind of method of capsicum leaf extract preparation enzyme beverage according to claim 2, it is characterised in that described Lactic acid bacteria is one or more of lactobacillus plantarum, leuconostoc pseudomesenteroides, lactobacillus acidophilus, and the saccharomycete is wine brewing One of yeast, Hansenula anomala and Bayer Zygosaccharomyces are a variety of.
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Application publication date: 20190806