CN110068560B - 一种受激辐射损耗超分辨成像***及方法 - Google Patents
一种受激辐射损耗超分辨成像***及方法 Download PDFInfo
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- 238000003384 imaging method Methods 0.000 title claims abstract description 122
- 230000005855 radiation Effects 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title abstract description 9
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- 238000001914 filtration Methods 0.000 claims description 16
- 230000010287 polarization Effects 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 11
- 238000012216 screening Methods 0.000 claims description 4
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- 239000000835 fiber Substances 0.000 description 8
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- 238000005516 engineering process Methods 0.000 description 4
- 101100028789 Arabidopsis thaliana PBS1 gene Proteins 0.000 description 2
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- 101100028790 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PBS2 gene Proteins 0.000 description 2
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- 238000002834 transmittance Methods 0.000 description 2
- 238000010870 STED microscopy Methods 0.000 description 1
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- 238000012634 optical imaging Methods 0.000 description 1
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- 238000010869 super-resolution microscopy Methods 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111474150B (zh) * | 2020-04-08 | 2022-03-22 | 华南师范大学 | 一种sted超分辨图像背景噪声差分抑制方法 |
CN111579486B (zh) * | 2020-06-04 | 2021-02-26 | 深圳大学 | 基于低功率受激发射损耗的超分辨成像方法及成像*** |
CN111589478B (zh) * | 2020-06-05 | 2021-06-29 | 珠海市尚维高科生物技术有限公司 | 一种双通道实时荧光定量pcr仪光路***及检测方法 |
CN114280020A (zh) * | 2021-12-21 | 2022-04-05 | 鑫海合星科技(大连)有限公司 | 一种基于空间光调制器的超分辨显微成像装置及方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105241857A (zh) * | 2015-09-30 | 2016-01-13 | 深圳大学 | 一种超分辨成像*** |
CN107045187A (zh) * | 2017-03-17 | 2017-08-15 | 王富 | 多光子超分辨显微成像装置及方法 |
CN107490568A (zh) * | 2017-08-09 | 2017-12-19 | 四川大学 | 基于受激发射损耗特性的超分辨率显微成像装置和方法 |
CN108957720A (zh) * | 2018-09-26 | 2018-12-07 | 中国科学院化学研究所 | 用于sted光学显微镜的照明***及sted光学显微镜 |
CN109211871A (zh) * | 2018-11-26 | 2019-01-15 | 深圳大学 | 一种受激发射损耗荧光寿命超分辨成像装置 |
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DE10340964A1 (de) * | 2003-09-05 | 2005-03-31 | Leica Microsystems Heidelberg Gmbh | Lichtquelle mit einem mikrostrukturierten optischen Element |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105241857A (zh) * | 2015-09-30 | 2016-01-13 | 深圳大学 | 一种超分辨成像*** |
CN107045187A (zh) * | 2017-03-17 | 2017-08-15 | 王富 | 多光子超分辨显微成像装置及方法 |
CN107490568A (zh) * | 2017-08-09 | 2017-12-19 | 四川大学 | 基于受激发射损耗特性的超分辨率显微成像装置和方法 |
CN108957720A (zh) * | 2018-09-26 | 2018-12-07 | 中国科学院化学研究所 | 用于sted光学显微镜的照明***及sted光学显微镜 |
CN109211871A (zh) * | 2018-11-26 | 2019-01-15 | 深圳大学 | 一种受激发射损耗荧光寿命超分辨成像装置 |
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