CN110066738A - A kind of AM fungal inoculant and its application method - Google Patents
A kind of AM fungal inoculant and its application method Download PDFInfo
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Abstract
The application provides a kind of AM fungal inoculant and its application method, is related to mine recovery technique field.A kind of AM fungal inoculant, including AM fungi culture medium and host plant root system, AM fungi culture medium include bacterium culture medium and AM fungi, bacterium culture medium includes the component of following parts by weight: 20~50 parts of zeolite, 5~30 parts of vermiculite, 5~30 parts of river sand, 2~5 parts of water-retaining agent.The application method of above-mentioned AM fungal inoculant includes the following steps: that S1 sequentially adds mine matrix modifier, mine soil and AM fungal inoculant in plantation hole；Mine soil in nursery stock transplanting to plantation hole, will be added again in S2, and carries out AM fungal infection plantation.The application can guarantee to expand numerous strain in the adaptability in semiarid mining area, effectively improve activity and sporulation quantity that microbial inoculum uses rear strain；Meanwhile the nutrient and water regime of Diggings soil can be significantly improved, the biomass of vegetation is improved, the regeneration rate and quality of mining area vegetation are accelerated.
Description
Technical field
This application involves mine recovery technique field, in particular to a kind of AM fungal inoculant and its application method.
Background technique
The development and utilization of mineral resources is that China's rapid development of economy and Urbanization Construction are made that great contribution,
But its extensive style, which is excessively developed, has also caused a series of environmental problems, seriously destroys local ecological environment.Cause sand and dust weather
Gas and water soil flow is lost, and causes the natural geologic hazards such as landslide, landslide, causes ecosystem degradation even to collapse, seriously threatens week
The sustainable development and region ecology safety in exclosure area.The ecology of mining areas system restored and rebuild degeneration is extremely urgent.
Revegetation is the important link of ecological restoration of mine, and revegetation is not only to playing the initial vegetation of building discardedly
Effect, moreover it is possible to improve soil texture, nutrient situation and microbial environment, promote the extensive of entire ecosystem structure and function
It is multiple.Mining soil is largely disturbed, and by the mechanical destruction excavated or carry, physics, chemical and biological activity are poor,
And its soil texture wrecks, land fertility is poor, water deficient, loses productivity, biology, microorganism growing environment are sharply
Deteriorate, vegetation is difficult to closing after being difficult to survive or survive and grows into forest.AM fungi can establish altogether as one kind with root system of plant
The strain of raw relationship, can help the absorption of plant absorption moisture and nutrient, especially phosphorus and microelement, enhance Genes For Plant Tolerance
Sick, degeneration-resistant, drought resistance reduces the loss of nitrogen phosphorus, improves plant to the resilience of heavy metal pollution；AM fungi is repaired in mine
Field, application potential are huge.
It is presently disclosed to expand numerous and application method mostly not directed to mining area about AM fungi.Application No. is
CN00132468.3, the patent application document of entitled " using bead as the culture of the arbuscular mycorrhizal fungi of culture substrate ", mentions
A kind of specific culture apparatus and culture medium are supplied；Application No. is CN02159722.7, entitled " production arbuscular mycorrhizal fungi
A variety of host plant mixed seeding, are mixed up on mixed culture substrate that produce AM true by the patent application document of the method for microbial inoculum "
Bacterium etc.；Application No. is CN201610990849.7, entitled " a kind of expanding propagation method of coal field bush mycorrhizal fungi preparation "
Patent application document, mainly joined weathered coal in culture substrate, and matrix is easy to get.But the side of these culture AM fungies
Method not can guarantee fungi and play effect to greatest extent under the severe life condition in mining area, and be more suitable for single type mining area
Application, application range is limited.
Summary of the invention
In view of this, the embodiment of the present application provides a kind of AM fungal inoculant and its application method, to solve the prior art
Present in technological deficiency.
A kind of AM fungal inoculant, including AM fungi culture medium and host plant root system, AM fungi culture medium include strain training
It supports base and AM fungi, bacterium culture medium includes the component of following parts by weight: 20~50 parts of zeolite, 5~30 parts of vermiculite, river sand 5
~30 parts, 2~5 parts of water-retaining agent.
Further, host plant root system include corn, sorghum, clover, white clover, clover, any two in Chinese herbaceous peony
Kind or three kinds of root system.
Further, AM fungi is that Gen Neigen sporangiocyst is mould.
Further, water-retaining agent includes inorganic water-retaining agent sodium bentonite, organic water retainer Sodium Polyacrylate, polyacrylic acid
Any one in potassium, starch grafted acrylate and water preservation.
A kind of application method of above-mentioned AM fungal inoculant, includes the following steps:
S1. mine matrix modifier, mine soil and AM fungal inoculant are sequentially added in plantation hole；
S2. mine soil in nursery stock transplanting to plantation hole, will be added again, and carry out AM fungal infection plantation.
Further, the mine matrix modifier in step S1, the component including following parts by weight: water-retaining agent 5~10
Part, 15~40 parts of bentonite, 20~40 parts of cinder, 15~30 parts of animal wastes.
Further, water-retaining agent includes inorganic water-retaining agent sodium bentonite, organic water retainer Sodium Polyacrylate, polyacrylic acid
Any one in potassium, starch grafted acrylate and water preservation.
Further, animal wastes include one or more of pig manure, cow dung, sheep dung, chicken manure, duck excrement, goose excrement.
Further, the step S1, comprising:
Mine matrix modifier is added in plantation hole to the 1/10~1/6 of plantation hole depth；
Mine soil is added in plantation hole, wherein the additional amount of mine soil adds with the mine matrix modifier
It is identical to enter amount；
AM fungal inoculant is sprinkled into plantation hole, wherein the mine matrix modifier, mine soil and AM fungal inoculant
Total height to the 1/3 of plantation hole depth at.
Method of the present invention has the advantages that compared with prior art
Expand numerous strain in the adaptability in semiarid mining area 1. ensure that, can effectively improve the activity that microbial inoculum uses rear strain
And sporulation quantity；
2. the nutrient and moisture shape of Diggings soil can be significantly improved after mine matrix modifier and Inoculation of Arbuscular Mycorrhizal Fungi is added
Condition improves the biomass of vegetation, accelerates the regeneration rate and quality of mining area vegetation；
3. being suitable for a plurality of types of mining areas, have a wide range of application.
Detailed description of the invention
Fig. 1 is the step schematic diagram of the application AM fungal inoculant application method.
Specific embodiment
Here is that the specific embodiment of the application is described.
AM fungi in the application is a kind of soil fungi, can establish symbiosis with 80% or more land plant root system
Relationship, forms arbuscular mycorrhiza, and AM fungi can promote suction of the root system to Soil Mineral Nutrition after forming mycorhiza with plant
Receipts, the water metabolism situation for improving plant, the anti-/ patience for improving plant pair heavy metal stress, the disease resistance for enhancing plant etc..
Zeolite in the application is the general name of zeolite race mineral, is a kind of aqueous alkali or alkaline earth metal aluminosilicate mine
Object, zeolite have ion exchangeable, adsorbing separation, catalytic, stability, chemical reactivity, reversible dehydration property, electrical conductance
Deng.
Vermiculite in the application, is a kind of natural, inorganic, nontoxic minerals, the dilatable mineral under high temperature action,
Belong to silicate.
River sand in the application, be natural stone in its natural state, active force through water collides repeatedly for a long time, rubbing produces
Raw, composition is complex, there is certain slickness on surface, the non-metallic ore more than impurity content.
Water-retaining agent in the application, uses super absorbent resin, is a kind of function high score that water absorbing capacity is especially strong
Sub- material, it is nontoxic, water, water suction are released repeatedly, therefore agriculturally it is likened to " miniature reservoir " by people.Meanwhile it can also
Fertilizer, pesticide and slow release are absorbed, fertilizer efficiency, drug effect are increased.
Corn in the application is grass family Zea annual herb plant, is important cereal crops and feed
Crop and the highest crops of whole world total output, protein rich in, fat, vitamin, microelement, fiber
Element etc..
Sorghum in the application is grass family annual herb plant, happiness temperature, light, and has certain high-temperature stability.
Clover in the application is the common name of clover, is commonly called as bur clover, is a kind of herbaceos perennial.Lucerne
Mu is many kinds of, is mostly wild herbaceous plant, drought-resistant like clover, cold-hot, and yield is high and of fine quality, and can conditioned soil
Earth.
White clover also known as Trifolium repense, white flower clover in the application, Bai Sancao, clover, Holland Astragalus sinicus, more
Year raw herbaceous plant, wide adaptability, heat resistanceheat resistant winter resistance is strong, can the vigorous growth in acid soil, can also be grown in sand soil,
Also there is certain ornamental value.On the taproot and lateral root of white clover have a large amount of root nodules, the nitrogen being capable of fixing in air,
It is not required to applied nitrogen in planting process, only a little phosphorus potash fertilizers need to be applied, so that with luxuriant foliage and spreading branches in leafy profusion.
Clover in the application, be it is a variety of possess three go out finger-like compound leaf herbal common names, mainly include three classes:
Certain types in the Clover and clover category of pulse family, the Oxalis of Oxalidaceae.Clover can increase the soil organism,
Advantageous culture fertility can reduce the summer soil moisture, stablize orchard air humidity, be conducive to summer fruit tree growth, clover can also
Good soil texture is kept, due to clover good covering effect, it is possible to prevente effectively from rainwater washing away and sink to soil
Product effect, keeps soil texture good, is conducive to the activity of edaphon and the metabolism of fruit tree root system, is conducive to various
The decomposition of fertilizer and the degradation of harmful substance.
Chinese herbaceous peony in the application is biennial or herbaceos perennial.Asiatic plantain is adaptable, resists cold, is drought-enduring, to soil
Earth require it is not tight, it is warm, moist, on the sunny side, can well-grown on sandy fertile soil.
Bentonite in the application is the nonmetallic minerals using montmorillonite as main mineral constituent.Bentonite can be divided into sodium
Base bentonite (alkaline soil), calcium-base bentonite (alkaline earth soil), natural bleaching are native (acid soil or Emathlite), and wherein calcium base is swollen
Profit soil includes calcium sodium base and calcium and magnesium base etc. again.Bentonite has strong hygroscopicity and dilatancy, and adsorbable 8~15 times from body
Long-pending water, volume expansion is up to several times to 30 times；It can be dispersed into as a form of gel and suspension, this medium solution in an aqueous medium
With certain viscosity, thixotropy and lubricity；There is stronger cation exchange capacity (CEC)；To various gases, liquid, organic matter
Matter has certain adsorption capacity, and maximal absorptive capacity is up to 5 times of itself weight；The admixture of it and water, mud or fine sand has
Plasticity and cohesiveness；Acid bleaching soil with surface-active can adsorb coloured ion.
Cinder in the application, is one kind of industrial solid wastes, main component be silica, aluminium oxide, iron oxide,
Calcium oxide, magnesia etc..
Animal wastes in the application, and be commonly called as defecating, it is the large intestine defecation object of animal, a quarter of excrement is water
Point, remaining is that protein, inorganic matter, fat, indigested food fiber, dewatered digestive juice are remaining and from intestines mostly
The cell and the bacterium to die that road falls off, there are also vitamin Ks, vitamin B.
Embodiment 1
A kind of AM fungal inoculant, including AM fungi culture medium and host plant root system, AM fungi culture medium include strain training
It supports base and AM fungi, bacterium culture medium includes the component of following parts by weight: 20~50 parts of zeolite, 5~30 parts of vermiculite, river sand 5
~30 parts, 2~5 parts of water-retaining agent.
Further, host plant root system includes the root system that the plant of symbiosis can be established with AM fungi.Host plants
Object root system includes corn, sorghum, clover, white clover, clover, any two or three of root system in Chinese herbaceous peony.
Further, AM fungi is that Rhizophagus intraradices Gen Neigen sporangiocyst is mould.
Further, water-retaining agent includes inorganic water-retaining agent sodium bentonite, organic water retainer Sodium Polyacrylate, polyacrylic acid
Any one in potassium, starch grafted acrylate and water preservation.
The cultural method of above-mentioned AM fungal inoculant, comprising the following steps:
(1) proportionally zeolite, vermiculite, river sand and water-retaining agent are uniformly mixed, and are configured to bacterium culture medium, strain
Culture medium is using preceding use60The sterilizing of Co gamma-ray irradiation；
(2) suitable host plant seed is chosen, and uses 10%H2O215min is impregnated, deionized water is rinsed to complete and rinsed
Completely, it is then sprouted on the filter paper of sterilizing, vernalization under 25 DEG C of dark condition；
(3) will sterilizing complete bacterium culture medium be added into potting, and if be put into dry granular sprout vernalization after host plant
Species, the pure culture Rhizophagus intraradices Gen Neigen sporangiocyst for being sprinkled into market purchase is mould, according to being put into group
Divide the 15% of gross mass deionized water is added, finally in seed upper layer covering 1cm or so bacterium culture medium, during which optionally pours
Water, and one time of nutrition liquid is poured every two weeks；
(4) culture 4~6 months, collect the AM fungi culture medium obtained after culture and host plant root system is used as and expands numerous obtain
The AM fungal inoculant arrived.
Water-retaining agent is added in the application innovation in bacterium culture medium, though water-retaining agent is not soluble in water, can absorb quite certainly
Body weight can be effectively suppressed moisture evaporation and can largely press down after penetrating into water-retaining agent in soil at hundred times of water
The evaporation of moisture processed reduces the saturated hydraulic conductivity of soil to improve saturated soil water content, to slow down soil release water
Speed reduces the infiltration and loss of soil moisture, achievees the purpose that water conservation.
Meanwhile water-retaining agent can also stimulate the growth and development of crop root, make that the length of root increases, item number increases,
Preferable growing way is kept under drought condition.It, can be by solubilityization since water-retaining agent has the function of absorbing and saving moisture
Nutriment needed for the crop growths such as fertilizer, pesticide is fixed wherein to reduce the loss of soluble nutrient to a certain extent,
The utilization rate for improving chemical fertilizer, pesticide etc., improves the growing way situation of crops.
Water-retaining agent has good thermal insulation property, after water-retaining agent, using water tariff collection part daytime of absorption
The Thermoregulation nocturnal temperature that illumination generates, so that soil day and night temperature reduces.
Water-retaining agent can also improve soil texture, during water-retaining agent is manured into soil, with the rule of water swelling and dehydration shrinkage
The variation of rule property, can make surrounding soil become loose from consolidation, and hole increases, to improve the penetrating shape of soil to a certain extent
Condition.
Embodiment 2
A kind of application method of above-mentioned AM fungal inoculant includes the steps that as described below referring to Fig. 1:
S1. mine matrix modifier, mine soil and AM fungal inoculant are sequentially added in plantation hole；
S2. mine soil in nursery stock transplanting to plantation hole, will be added again, and carry out AM fungal infection plantation.
Further, the mine matrix modifier in step S1, the component including following parts by weight: water-retaining agent 5~10
Part, 15~40 parts of bentonite, 20~40 parts of cinder, 15~30 parts of animal wastes.
Further, water-retaining agent includes inorganic water-retaining agent sodium bentonite, organic water retainer Sodium Polyacrylate, polyacrylic acid
Any one in potassium, starch grafted acrylate and water preservation.
Further, animal wastes include one or more of pig manure, cow dung, sheep dung, chicken manure, duck excrement, goose excrement.
Further, mine matrix modifier is added in plantation hole to the 1/10~1/6 of plantation hole depth；
Mine soil is added in plantation hole, wherein the additional amount of mine soil adds with the mine matrix modifier
It is identical to enter amount；
AM fungal inoculant is sprinkled into plantation hole, wherein the mine matrix modifier, mine soil and AM fungal inoculant
Total height to the 1/3 of plantation hole depth at.
It similarly joined water-retaining agent, mine matrix modifier and AM fungi bacterium in mine matrix modifier in the application
Agent is successively added in plantation hole, and double water-retaining agent can play dual water retention, can play its rush for AM fungi
Double guarantee is provided into effect of the root system of plant to the absorption of nutritional ingredient etc..
Further, water-retaining agent can also be played with AM fungal inoculant and be acted synergistically, and water-retaining agent can be effectively improved soil
Environment guarantees the water content in soil, reduces the infiltration of soil moisture, is lost, so that the loss of nutritional ingredient in soil is reduced,
And then enhance AM fungal inoculant to the function and effect of soil and seedling root.
Further, the presence of water-retaining agent can effectively expand the scope of application of the application, so that AM fungal inoculant is not
It is only suitable for the mining area of single type again, while can guarantees to play work to greatest extent under the severe life condition in mining area
With improving the biomass of vegetation, accelerate the regeneration rate and quality of mining area vegetation.
Test example 1
Processing group and control group is arranged simultaneously in this test example, wherein processing group is handled using method as described below:
(1) prepare bacterium culture medium, the component including following parts by weight: 45 parts of zeolite, 25 parts of vermiculite, 25 parts of river sand,
It 5 parts of water-retaining agent, is uniformly mixed, the water-retaining agent selected in the present embodiment is polyacrylic acid potassium, and bacterium culture medium is using preceding use60Co
Gamma-ray irradiation sterilizing；
Corn seed and alfalfa seed are chosen as host plant seed, and uses 10%H2O2Impregnate 15min, deionized water
8 times are rinsed to rinsing well completely, is then sprouted on the filter paper of sterilizing, vernalization under 25 DEG C of dark condition.
The AM fungi culture medium that sterilizing is completed is added to the 2/3 of potting, 4 corn seeds and 15 sprouted are put into
Grain alfalfa seed, is sprinkled into the pure culture Rhizophagusintraradices arbuscular mycorrhizal fungi of market purchase, according to being put into
Deionized water is added in the 15% of component gross mass, finally in seed upper layer covering 1cm or so bacterium culture medium, during which regards arid feelings
It pours a water within condition every 3~5 days, pours the Huo Gelan nutrient solution (as shown in table 1) that a pH is 5.5~6.0 every two weeks；
The Glan compounding component list suddenly of table 1.
Note: mother liquor (ml) needed for a most right column indicate configuration 1L nutrient solution.
(2) it cultivates 5 months, the AM fungi culture medium and host plant root system after collecting culture are true as numerous obtained AM is expanded
Bacteria agent.
(3) nursery transplant of Sabina vulgaris is subjected to the plantation of AM fungal infection in semiarid mining area.
Plantation hole has been dug according to the root system size of Sabina vulgaris nursery transplant, having a size of 50cm (diameter) * 40cm (height), has been applied first
Enter at mine matrix modifier to the above 4cm in plantation hole bottom surface, then apply with the same amount of mine soil of mine matrix modifier,
It is then sprinkled at AM fungal inoculant to the 1/3 of plantation hole depth, the Sabina vulgaris through water-retaining agent root immersion of 20~30cm is moved
Transplanting plant seedlings in plantation hole, backfills mine soil, and treaded soil with foot, pours primary permeable, supplement is applied again after 5 months
Add AM fungal inoculant.
Wherein, the mine matrix modifier used in the present embodiment, the component including following parts by weight: polyacrylic acid potassium
5 parts, 30 parts of bentonite, 40 parts of cinder, 25 parts of goose excrement.
Control group only adds common mine matrix modifier using traditional method for recovering vegetation during processing, without
Apply AM fungal inoculant, other Step By Conditions are identical as above-mentioned processing group.
Seeding row spacing between Sabina vulgaris nursery transplant is 20cm*30cm.
After Sabina vulgaris is transplanted 1 year, the dip dyeing situation and upgrowth situation of Sabina vulgaris root system are detected, 2 institute of table is obtained
Registration evidence.
Sabina vulgaris root system infects frequency and infection strength under 2. different disposal of table
From table 2 it can be seen that the dip dyeing frequency of processing group Sabina vulgaris root system and dip dyeing intensity are apparently higher than control group, that is, adopt
With New Mine method for recovering vegetation of the present invention, the dip dyeing frequency and intensity medicine of Sabina vulgaris root system are significantly higher than tradition side
Method, this illustrates that the AM fungal inoculant applied in treatment process has disseminated Sabina vulgaris root system, and forms the homobium of mutual reciprocity and mutual benefit,
And in the case where conventional method does not apply AM fungal inoculant, there is also infecting on a small quantity, this is because Mine land itself is deposited
In indigenous AM fungi, but fungi is less.
Sabina vulgaris is grown 1 year or so, is counted to its survival rate and growing way situation, the sand of discovery conventional method plantation
Ground cypress survival rate is relatively low, and only 50%~65% or so, and foliage portion is withered and yellow；And use the sand of the method for the present invention plantation
Ground cypress survival rate is significantly improved to 95% or more, and this part Sabina vulgaris grows fine, flourishing.It can be seen that of the invention
It is strong to expand numerous AM fungi strain proliferative capacity, the outer mycelia of root can establish symbiosis with Sabina vulgaris root system rapidly, can be compared with
The environment in mine is adapted to well, improves plant percent, meanwhile, it can be together with the water-retaining agent of application and mine matrix modifier
The nutrient and water regime for improving Diggings soil, improve the biomass of vegetation, are conducive to the recovery of mining area vegetation.
Test example 2
Processing group and control group is arranged simultaneously in this test example, wherein processing group is handled using method as described below:
(1) prepare bacterium culture medium, the component including following parts by weight: 40 parts of zeolite, 30 parts of vermiculite, 27 parts of river sand,
It 3 parts of water-retaining agent, is uniformly mixed, the water-retaining agent selected in the present embodiment is sodium bentonite, and bacterium culture medium is using preceding use60Co
Gamma-ray irradiation sterilizing；
Corn seed and Chinese herbaceous peony seed are chosen as host plant seed, and uses 10%H2O2Impregnate 15min, deionized water
8 times are rinsed to rinsing well completely, is then sprouted on the filter paper of sterilizing, vernalization under 25 DEG C of dark condition.
The AM fungi culture medium that sterilizing is completed is added to the 2/3 of potting, 4 corn seeds and 15 sprouted are put into
Grain Chinese herbaceous peony seed, is sprinkled into the pure culture Rhizophagusintraradices arbuscular mycorrhizal fungi of market purchase, according to being put into
Deionized water is added in the 15% of component gross mass, finally in seed upper layer covering 1cm or so bacterium culture medium, during which regards arid feelings
It pours a water within condition every 3~5 days, pours the Huo Gelan nutrient solution that a pH is 5.5~6.0, the preparation of Huo Gelan nutrient solution every two weeks
As shown in the table 1 in embodiment 1.
(2) it cultivates 5 months, the AM fungi culture medium and host plant root system after collecting culture are true as numerous obtained AM is expanded
Bacteria agent.
(3) nursery transplant of Sabina vulgaris is subjected to the plantation of AM fungal infection in semiarid mining area.
Plantation hole has been dug according to the root system size of Sabina vulgaris nursery transplant, having a size of 50cm (diameter) * 40cm (height), has been applied first
Enter at mine matrix modifier to the above 4cm in plantation hole bottom surface, then apply with the same amount of mine soil of mine matrix modifier,
It is then sprinkled at AM fungal inoculant to the 1/3 of plantation hole depth, the Sabina vulgaris through water-retaining agent root immersion of 20~30cm is moved
Transplanting plant seedlings in plantation hole, backfills mine soil, and treaded soil with foot, pours primary permeable, supplement is applied again after 5 months
Add AM fungal inoculant.
Wherein, the mine matrix modifier used in the present embodiment, the component including following parts by weight: sodium bentonite
7 parts, 33 parts of bentonite, 30 parts of cinder, 30 parts of duck excrement.
Control group only adds common mine matrix modifier using traditional method for recovering vegetation during processing, without
Apply AM fungal inoculant, other Step By Conditions are identical as above-mentioned processing group.
Seeding row spacing between Sabina vulgaris nursery transplant is 20cm*30cm.
After Sabina vulgaris is transplanted 1 year, the dip dyeing situation and upgrowth situation of Sabina vulgaris root system are detected, 3 institute of table is obtained
Registration evidence.
Sabina vulgaris root invasion frequency and infection strength under 3. different disposal of table
From table 3 it can be seen that the dip dyeing frequency and dip dyeing intensity of processing group are all apparently higher than after Sabina vulgaris is transplanted 1 year
Control group, using method of the invention, the dip dyeing frequency and dip dyeing intensity of Sabina vulgaris root system have all reached 85% or more, and use
The dip dyeing frequency and dip dyeing intensity of the Sabina vulgaris root system of conventional method illustrate that the AM fungal inoculant applied can only 10% or so
To form mutually beneficial homobium with Sabina vulgaris root system.
Meanwhile by Sabina vulgaris survive quantity and growing way situation counts, discovery use method kind of the invention
The Sabina vulgaris survival rate of plant grows fine 95% or more, and the Sabina vulgaris some leaf of conventional method plantation is withered and yellow, survival rate
It is relatively low, only 60% or so.It can be seen that method of the invention be more conducive to mine vegetation survive and mine soil
Recovery.
Test example 3
Processing group and control group is arranged simultaneously in this test example, wherein processing group is handled using method as described below:
(1) prepare bacterium culture medium, the component including following parts by weight: 50 parts of zeolite, 25 parts of vermiculite, 20 parts of river sand,
It 5 parts of water-retaining agent, is uniformly mixed, the water-retaining agent selected in the present embodiment is Sodium Polyacrylate, and bacterium culture medium is using preceding use60Co
Gamma-ray irradiation sterilizing；
Sorghum seeds and alfalfa seed are chosen as host plant seed, and use 10%H2O2Impregnate 15min, deionized water
8 times are rinsed to rinsing well completely, is then sprouted on the filter paper of sterilizing, vernalization under 25 DEG C of dark condition.
The AM fungi culture medium that sterilizing is completed is added to the 2/3 of potting, 4 sorghum seeds and 15 sprouted are put into
Grain alfalfa seed, is sprinkled into the pure culture Rhizophagusintraradices arbuscular mycorrhizal fungi of market purchase, according to being put into
Deionized water is added in the 15% of component gross mass, finally in seed upper layer covering 1cm or so bacterium culture medium, during which regards arid feelings
It pours a water within condition every 3~5 days, pours the Huo Gelan nutrient solution that a pH is 5.5~6.0, the preparation of Huo Gelan nutrient solution every two weeks
As shown in the table 1 in embodiment 1.
(2) it cultivates 6 months, the AM fungi culture medium and host plant root system after collecting culture are true as numerous obtained AM is expanded
Bacteria agent.
(3) nursery transplant of Sabina vulgaris is subjected to the plantation of AM fungal infection in semiarid mining area.
Plantation hole has been dug according to the root system size of Sabina vulgaris nursery transplant, having a size of 50cm (diameter) * 40cm (height), has been applied first
Enter at mine matrix modifier to the above 4cm in plantation hole bottom surface, then apply with the same amount of mine soil of mine matrix modifier,
It is then sprinkled at AM fungal inoculant to the 1/3 of plantation hole depth, the Sabina vulgaris through water-retaining agent root immersion of 20~30cm is moved
Transplanting plant seedlings in plantation hole, backfills mine soil, and treaded soil with foot, pours primary permeable, supplement is applied again after 5 months
Add AM fungal inoculant.
Wherein, the mine matrix modifier used in the present embodiment, the component including following parts by weight: Sodium Polyacrylate
10 parts, 30 parts of bentonite, 20 parts of cinder, 40 parts of chicken manure.
Control group only adds common mine matrix modifier using traditional method for recovering vegetation during processing, without
Apply AM fungal inoculant, other Step By Conditions are identical as above-mentioned processing group.
Seeding row spacing between Sabina vulgaris nursery transplant is 20cm*30cm.
After Sabina vulgaris is transplanted 1 year, the dip dyeing situation and upgrowth situation of Sabina vulgaris root system are detected, 4 institute of table is obtained
Registration evidence.
Sabina vulgaris root invasion frequency and infection strength under 4. different disposal of table
As a result trend is reached after Sabina vulgaris is transplanted 1 year using method of the invention with above-mentioned test example 1 and test example 2
To effect be substantially better than conventional method.As shown in table 4, using the processing group Sabina vulgaris of the method for the present invention, AM fungal infection frequency
90% or more degree, infection strength also reaches 85% or more, and the death rate of Sabina vulgaris is even more less than 5%.Using conventional method kind
The control group of plant depends on original inhabitants AM fungi, and Sabina vulgaris root system infects frequency about 14%, and infection strength maintains 10% left side
The right side, Sabina vulgaris plant strain growth is bad, survival rate about 60%.The comparison of two kinds of recovering plant cover of mine modes has absolutely proved application
AM fungal inoculant symbiosis that good mutual reciprocity and mutual benefit can be established with Sabina vulgaris root system and mine matrix modifier together
The nutrient and water regime of Diggings soil are significantly improved, the survival rate and biomass of vegetation are improved, to the extensive of mining area vegetation
It is of great significance again.
Herein, " first ", " second " etc. are only used for mutual differentiation, rather than indicate significance level and sequence and
Existing premise etc. each other.
Herein, the limitation in " equal ", " identical " etc. and non-critical mathematics and/or geometry meaning also includes
It will be appreciated by those skilled in the art that and the error that the permissions such as makes or uses.
Unless otherwise indicated, numberical range herein not only includes the entire scope in two endpoint, also includes containing
In several subranges therein.
The application preferred embodiment and embodiment are explained in detail above, but the application is not limited to
The above-described embodiment and examples can also not depart from the application within the knowledge of those skilled in the art
It is made a variety of changes under the premise of design.
Claims (9)
1. a kind of AM fungal inoculant, it is characterised in that: including AM fungi culture medium and host plant root system, the AM fungal culture
Base includes bacterium culture medium and AM fungi, and the bacterium culture medium includes the component of following parts by weight: 20~50 parts of zeolite, leech
5~30 parts of stone, 5~30 parts of river sand, 2~5 parts of water-retaining agent.
2. AM fungal inoculant according to claim 1, it is characterised in that: the host plant root system include corn, sorghum,
Clover, white clover, clover, any two or three of root system in Chinese herbaceous peony.
3. AM fungal inoculant according to claim 1, it is characterised in that: the AM fungi is that Gen Neigen sporangiocyst is mould.
4. AM fungal inoculant according to claim 1, it is characterised in that: the water-retaining agent includes that inorganic water-retaining agent sodium base is swollen
It is any one in profit soil, organic water retainer Sodium Polyacrylate, polyacrylic acid potassium, starch grafted acrylate and water preservation
Kind.
5. a kind of application method of such as described in any item AM fungal inoculants of Claims 1 to 4, which is characterized in that including as follows
Step:
S1. mine matrix modifier, mine soil and AM fungal inoculant are sequentially added in plantation hole；
S2. mine soil in nursery stock transplanting to plantation hole, will be added again, and carry out AM fungal infection plantation.
6. the application method of AM fungal inoculant according to claim 5, it is characterised in that: the mine base in the step S1
The component including following parts by weight: quality improving agent 5~10 parts of water-retaining agent, 15~40 parts of bentonite, 20~40 parts of cinder, moves
15~30 parts of object excrement.
7. the application method of AM fungal inoculant according to claim 6, it is characterised in that: the water-retaining agent includes inorganic guarantor
Aqua sodium bentonite, organic water retainer Sodium Polyacrylate, polyacrylic acid potassium, starch grafted acrylate and water preservation
In any one.
8. the application method of AM fungal inoculant according to claim 6, it is characterised in that: the animal wastes include pig
One or more of excrement, cow dung, sheep dung, chicken manure, duck excrement, goose excrement.
9. the application method of AM fungal inoculant according to claim 5, which is characterized in that the step S1, comprising:
Mine matrix modifier is added in plantation hole to the 1/10~1/6 of plantation hole depth；
Mine soil is added in plantation hole, wherein the additional amount of the additional amount of mine soil and the mine matrix modifier
It is identical；
AM fungal inoculant is sprinkled into plantation hole, wherein the mine matrix modifier, mine soil and AM fungal inoculant it is total
At height to the 1/3 of plantation hole depth.
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| CN111466172A (en) * | 2020-04-08 | 2020-07-31 | 浙江省农业科学院 | Method for restoring cadmium-polluted soil by using arbuscular mycorrhizal fungi combined greening nursery stock |
| CN113115691A (en) * | 2021-04-22 | 2021-07-16 | 内蒙古大学 | Method for AM fungus trapping culture in arctic-alpine grassland |
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| CN106508429A (en) * | 2016-11-10 | 2017-03-22 | 中国矿业大学(北京) | Multiplication method of arbuscular mycorrhiza fungi inoculant in coal mine area |
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| CN111466172A (en) * | 2020-04-08 | 2020-07-31 | 浙江省农业科学院 | Method for restoring cadmium-polluted soil by using arbuscular mycorrhizal fungi combined greening nursery stock |
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| CN113115691A (en) * | 2021-04-22 | 2021-07-16 | 内蒙古大学 | Method for AM fungus trapping culture in arctic-alpine grassland |
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