CN110057821A - 用于人类配子快速冻融过程观测的低温显微成像*** - Google Patents
用于人类配子快速冻融过程观测的低温显微成像*** Download PDFInfo
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- CN110057821A CN110057821A CN201910302481.4A CN201910302481A CN110057821A CN 110057821 A CN110057821 A CN 110057821A CN 201910302481 A CN201910302481 A CN 201910302481A CN 110057821 A CN110057821 A CN 110057821A
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- 238000012424 Freeze-thaw process Methods 0.000 title claims abstract description 9
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8411—Application to online plant, process monitoring
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- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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CN201910302481.4A CN110057821B (zh) | 2019-04-16 | 2019-04-16 | 用于人类配子快速冻融过程观测的低温显微成像*** |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112684827A (zh) * | 2020-12-25 | 2021-04-20 | 浙江大学 | 一种基于pid控制的恒温结霜冷面装置及其恒温控制方法 |
Citations (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1986004673A1 (en) * | 1985-02-08 | 1986-08-14 | Rijksuniversiteit Leiden | A method and a spectrometer for measuring absorbance difference spectra of radical reactions |
CN1162900A (zh) * | 1994-08-18 | 1997-10-22 | 全美红十字会 | 用于低温保存和随后移植的器官的制备方法 |
US20070125098A1 (en) * | 2001-11-01 | 2007-06-07 | Integrated Biosystems, Inc. | Systems and methods for freezing, storing and thawing biopharmaceutical material |
CN201436600U (zh) * | 2009-04-30 | 2010-04-07 | 上海交通大学医学院附属瑞金医院 | 一种低温生物显微*** |
CN101793458A (zh) * | 2010-04-02 | 2010-08-04 | 中山大学 | 具有dsc及微结构观察功能的真空冷冻干燥装置 |
CN102204527A (zh) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | 生物材料玻璃化冷冻保存承载工具 |
CN105044142A (zh) * | 2015-05-08 | 2015-11-11 | 上海交通大学 | 一种宽温区可控的材料热物性测量*** |
CN105579045A (zh) * | 2013-07-17 | 2016-05-11 | 低温药理Kf有限公司 | 用于器官停滞、保护和保存以及减少组织损伤的方法 |
CN106153622A (zh) * | 2015-04-22 | 2016-11-23 | 北京大学深圳医院 | 玻璃化冷冻观察装置及其应用 |
CN106370692A (zh) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | 深冷液体中微量可溶性杂质的溶解析出可视化观测装置 |
CN106707489A (zh) * | 2017-03-09 | 2017-05-24 | 中国科学院生物物理研究所 | 冷冻显微镜用超低温样品台 |
CN206238188U (zh) * | 2016-10-20 | 2017-06-13 | 上海市第一人民医院 | 一种液氮熏蒸箱 |
CN107372470A (zh) * | 2017-09-11 | 2017-11-24 | 马博 | 一种卵子胚胎玻璃化冷冻保存装置 |
CN107592789A (zh) * | 2015-04-23 | 2018-01-16 | 骨治疗公司 | 治疗性细胞的体外保存 |
CN208110696U (zh) * | 2018-04-03 | 2018-11-16 | 天津金诺恒信生物科技有限公司 | 一种组织库冻存管理装置 |
CN108873297A (zh) * | 2018-09-15 | 2018-11-23 | 乔燕春 | 一种显微镜用载物台及由其构成的冻干显微镜 |
CN109122663A (zh) * | 2018-09-04 | 2019-01-04 | 浙江海洋大学 | 一种用于长期保存太平洋真宽水蚤休眠卵的冻干冷藏法 |
-
2019
- 2019-04-16 CN CN201910302481.4A patent/CN110057821B/zh active Active
Patent Citations (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1986004673A1 (en) * | 1985-02-08 | 1986-08-14 | Rijksuniversiteit Leiden | A method and a spectrometer for measuring absorbance difference spectra of radical reactions |
CN1162900A (zh) * | 1994-08-18 | 1997-10-22 | 全美红十字会 | 用于低温保存和随后移植的器官的制备方法 |
US20070125098A1 (en) * | 2001-11-01 | 2007-06-07 | Integrated Biosystems, Inc. | Systems and methods for freezing, storing and thawing biopharmaceutical material |
CN201436600U (zh) * | 2009-04-30 | 2010-04-07 | 上海交通大学医学院附属瑞金医院 | 一种低温生物显微*** |
CN101793458A (zh) * | 2010-04-02 | 2010-08-04 | 中山大学 | 具有dsc及微结构观察功能的真空冷冻干燥装置 |
CN102204527A (zh) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | 生物材料玻璃化冷冻保存承载工具 |
CN105579045A (zh) * | 2013-07-17 | 2016-05-11 | 低温药理Kf有限公司 | 用于器官停滞、保护和保存以及减少组织损伤的方法 |
CN106153622A (zh) * | 2015-04-22 | 2016-11-23 | 北京大学深圳医院 | 玻璃化冷冻观察装置及其应用 |
CN107592789A (zh) * | 2015-04-23 | 2018-01-16 | 骨治疗公司 | 治疗性细胞的体外保存 |
CN105044142A (zh) * | 2015-05-08 | 2015-11-11 | 上海交通大学 | 一种宽温区可控的材料热物性测量*** |
CN106370692A (zh) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | 深冷液体中微量可溶性杂质的溶解析出可视化观测装置 |
CN206238188U (zh) * | 2016-10-20 | 2017-06-13 | 上海市第一人民医院 | 一种液氮熏蒸箱 |
CN106707489A (zh) * | 2017-03-09 | 2017-05-24 | 中国科学院生物物理研究所 | 冷冻显微镜用超低温样品台 |
CN107372470A (zh) * | 2017-09-11 | 2017-11-24 | 马博 | 一种卵子胚胎玻璃化冷冻保存装置 |
CN208110696U (zh) * | 2018-04-03 | 2018-11-16 | 天津金诺恒信生物科技有限公司 | 一种组织库冻存管理装置 |
CN109122663A (zh) * | 2018-09-04 | 2019-01-04 | 浙江海洋大学 | 一种用于长期保存太平洋真宽水蚤休眠卵的冻干冷藏法 |
CN108873297A (zh) * | 2018-09-15 | 2018-11-23 | 乔燕春 | 一种显微镜用载物台及由其构成的冻干显微镜 |
Non-Patent Citations (5)
Title |
---|
HUANG YONGHUA等: "《performance of cryogenic regenerator with 3He as working fluid》", 《CHINESE SCIENCE BULLETIN》 * |
SHOHEI YAMAMOTO: "《quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation》", 《INTERNATIONAL JOURNAL OF HEMATOLOGY》 * |
李维杰等: "《纳米低温保护剂提高***玻璃化保存效果的机理初探》", 《制冷学报》 * |
沈圆圆: "冷热交替条件下的肿瘤微循环损伤及机理研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
黄永华等: "《基于制冷机的稳态与动态低温温度计标定研究》", 《制冷技术》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112684827A (zh) * | 2020-12-25 | 2021-04-20 | 浙江大学 | 一种基于pid控制的恒温结霜冷面装置及其恒温控制方法 |
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Effective date of registration: 20220418 Address after: 201100 rooms 202, 205 and 303-304, building 2, No. 152, Lane 468, North hengshahe Road, Minhang District, Shanghai Patentee after: Shanghai Shaoqing Baishao Medical Technology Co.,Ltd. Address before: 200240 No. 800, Dongchuan Road, Shanghai, Minhang District Patentee before: SHANGHAI JIAO TONG University Patentee before: Shanghai First People's Hospital |
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Address after: Room 601, Building 1, No. 58, Tanzhu Road, Minhang District, Shanghai, 201100 Patentee after: Shanghai Elwin Life Technology Co.,Ltd. Address before: 201100 rooms 202, 205 and 303-304, building 2, No. 152, Lane 468, North hengshahe Road, Minhang District, Shanghai Patentee before: Shanghai Shaoqing Baishao Medical Technology Co.,Ltd. |
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Denomination of invention: A low-temperature microscopic imaging system for observing the rapid freezing and thawing process of human gametes Effective date of registration: 20230421 Granted publication date: 20210907 Pledgee: The Bank of Shanghai branch Caohejing Limited by Share Ltd. Pledgor: Shanghai Elwin Life Technology Co.,Ltd. Registration number: Y2023310000132 |
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Granted publication date: 20210907 Pledgee: The Bank of Shanghai branch Caohejing Limited by Share Ltd. Pledgor: Shanghai Elwin Life Technology Co.,Ltd. Registration number: Y2023310000132 |
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