CN110052159A - A kind of new type of safe high-performance bio deodorant and preparation method thereof - Google Patents
A kind of new type of safe high-performance bio deodorant and preparation method thereof Download PDFInfo
- Publication number
- CN110052159A CN110052159A CN201910423864.7A CN201910423864A CN110052159A CN 110052159 A CN110052159 A CN 110052159A CN 201910423864 A CN201910423864 A CN 201910423864A CN 110052159 A CN110052159 A CN 110052159A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- deodorant
- extract
- new type
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses efficient biological deodorant of a kind of new type of safe and preparation method thereof, the efficient biological deodorant of the new type of safe includes beneficial bacterium agent, plant extracts and biological enzyme；The beneficial microbial inoculum is made of lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria；The plant extracts is made of green-tea extract, grape seed extract, yucca extract and Celeryseed extract；The biological enzyme is aspartic protease and papain.The preparation method comprises the following steps: being cultivated lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria respectively to plateau, then mixing, which is added in culture medium, obtains total culture medium；It will be added in total culture medium beneficial to microbial inoculum again and be uniformly mixed, plant extracts finally is added in total culture medium, preservation getting the product is mixed evenly in biological enzyme.
Description
Technical field
The invention belongs to environment protection fields more particularly to a kind of efficient biological deodorant of new type of safe and preparation method thereof.
Background technique
In daily life, we can often smell one stink, and cause this strand of stink factor be it is diversified,
Not only there are various microorganism decomposition substances and own mortality bring stench, also by decomposing dissolution tannin in the water such as fallen leaves, water plant
It is sour and smelly etc..These stink substancess escape into and form foul smell in air, and foul smell is not only unpleasant also to generate danger to the smell of people
Evil, or even can cause environment pollution.Odorant in foul smell includes: sulfur-containing compound, such as: hydrogen sulfide, carbon disulfide, sulphur
Alcohol, thioether, methyl sulphur etc.；Nitrogenous compound, such as: ammonia, trimethylamine, amide, indoles；Hydro carbons, such as: alkane, alkene, alkynes,
Aromatic hydrocarbon, halogenated hydrocarbons etc., oxygen-containing organic compound, such as: alcohol, phenol, aldehyde ketone, organic acid.
With global warming, the haze weathers such as exceedingly odious are not increase and the regulation of national environmental protection policy is got over
Carry out severeer, the continuous enhancing of people's environmental consciousness, it is higher and higher with the requirement of the air quality of living environment for living.For
Foul smell in degradation rubbish, technical staff has invented various deodorant, and raises and degrade, but chemical deodorizing therein
Agent easily causes secondary pollution, and plant deodorant is short there are the time, is easy degradation, and at high cost, therefore scientific research personnel gradually opens
Begin to carry out deodorization using biological agent.But existing bacteria agent bacterial strain type is single, specific aim is not strong, and cannot effectively disappear
It goes out the helminths such as roundworm.
Existing deodorizing microorganism effect is irregular, and microbial inoculum kind bacterial strain type is single, and specific aim is not strong, particularly with change excrement
Pond, refuse landfill, the targeted deodorant that farm carries out deodorization is rare, so, in view of the above-mentioned problems, being badly in need of one
Kind can improve these place ecological environments, decomposition and convert pernicious gas and harmful microorganism is inhibited to grow, eliminate helminth and
The biological deodorant of its worm's ovum.
Summary of the invention
To solve the above problems, the present invention provides a kind of novel safe biologic deodorant and preparation method thereof.This hair
Bacterial strain in the bright biological deodorant being prepared has both anaerobism and aerobic bacterial strain, wide adaptation range, Nantural non-toxic, and by
Plant extracts and biological enzyme technology enable to deodorizing effect rapidly and efficiently lasting, and can effectively go out except the parasitism in rubbish
Worm's ovum.
The scheme of the invention is realize in this way:
A kind of efficient biological deodorant of new type of safe, including beneficial bacterium agent, plant extracts and biological enzyme；Described has
Beneficial bacteria agent is made of lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria；
The plant extracts is made of green-tea extract, grape seed extract, yucca extract and Celeryseed extract；Described
Biological enzyme is hydrolysising protease；The quality proportioning of the beneficial microbial inoculum, plant extracts, biological enzyme are as follows: 5.5-6:2:2-
2.5；The lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria it is effective
The ratio between viable count are as follows: 10:10:5:5:10:10:6-9, the green-tea extract, grape seed extract, yucca extract and celery
Seed extract effective mass proportion: 3-3.5:2-3:3:2-2.5；The beneficial microbial inoculum is dissolved in total culture medium, then again
Plant extracts and biological enzyme are added to be uniformly mixed in total culture medium and saved.
As a further explanation of the present invention, total living bacteria count of microbial inoculum is 10 in total culture medium8CFU/
ml。
As a further explanation of the present invention, the biological enzyme is made of aspartic protease and papain.
As a further explanation of the present invention, the aspartic protease and papain quality proportioning are 1:1-
1.5。
A method of preparing the efficient biological deodorant of new type of safe, comprising the following steps:
(1) by lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria
It is cultivated respectively to plateau, then mixing, which is added in culture medium, obtains total culture medium；
(2) it will be added in total culture medium in (1) beneficial to microbial inoculum again and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
As a further explanation of the present invention, the formula for preparing the culture medium is as follows: beef extract 12g, glucose
10g, peptone 12g, KH2PO42.5g, NaCl5g, water 1000ml, pH value adjustment to 6.0-6.5.
As a further explanation of the present invention, it is urged in total culture medium containing the deodorization that total concentration is 10-100ppm
Agent.
As a further explanation of the present invention, the deodorization catalyst includes aluminum nitrate and iron chloride, the nitric acid
The molar ratio of aluminium and iron chloride is 2:3.
The principle that the present invention realizes is:
Using in deodorant of the invention, foul gas can be dissolved in deodorant of the invention first, foul gas
It is the mass transport process that gas phase transition is liquid phase in decomposable process.Be dissolved in foul smell in deodorant water by the cell wall of microorganism and
Cell membrane is absorbed by the micro-organisms, and foul smell insoluble in water is first attached to outside microbial body, by biological enzyme and microorganism secretion
Exoenzyme is decomposed into solable matter, then penetrates into cell, is in vivo micro- life as nutriment after foul smell enters cell
Object decomposes, using, so that foul smell is removed.
The reaction principle of deodorant of the invention in use can be divided into following aspects:
Reaction principle one: biological deodorant surface can not only effectively absorption, decompose air in foul gas molecule, together
When also the spatial configuration for the odor molecules being adsorbed can be made to change, the chemical combination key in odor molecules is weakened, so that peculiar smell
The unstability of molecule increases, and is easy to be chemically reacted with other molecules, i.e., with the acidic buffer in deodorant of the invention
Liquid reacts, and ultimately produces tasteless, nontoxic organic salt.As hydrogen sulfide reacted under the action of deodorant generate sulfate radical from
Son and water；Ammonia generates nitrogen and water under the action of plant extracts.
Furthermore under the action of outer grenz rays, so that microorganism, biological enzyme and plant extract in deodorant of the present invention
The increased activity of active material in object can absorb different types of odorant, remove more easily in conjunction with odorant
Smelly effect is stronger.
Reaction principle two: making stench toxin be transformed into innocuous substance based on biochemical reaction and biophysics process, this
Body can be degradable by biology.Its reaction process is as shown in Figure 1.
Ambient temperature increases under field conditions (factors), and the biological deodorizing agent solution in the present invention is also with substrate temperature
Change also corresponding change, while foul smell molecule also accelerates to expand when temperature raising, strain in deodorant etc. can also increase accordingly
Expansion is added to breed, but the speed expanded at this time does not catch up with the concentration expansion of VOCs and foul gas, so need biological enzyme
Oxidation reaction, reaction process are as follows:
Catalytic oxidation: R-NH2+H2S→R-NH3 ++SH-+O2→R-NH3+SO4 2-+OH-
R-NH3 ++OH-→R-NH2+H2O
R-SH (air)+O2→ R-SS-R (slow)
R-SH (biological enzyme in deodorant liquid)+O2 → R-SS-R (fast)
Redox reaction: R-NH2+HCHO→R-HN2+ H-C=CO2+H2O
R-NH2+NH3→R-NH2+N2+H2O
Its reaction principle three: enzyme molecule is made of amino acid long chain.A portion chain is spiral, and a part is at folding
Flake structure, and this two parts is connected by not folded amino acid chain, and entire enzyme molecule is made to become specific three-dimensional
Structure, it has stronger catalytic decomposition efficiency.
The present invention has following good result:
1. the present invention is combined as deodorant using beneficial to microbial inoculum, plant extracts and biological enzyme.Having in the present invention
Strain in beneficial bacteria agent has both anaerobism and aerobic bacterial strain, adaptable, while high by the deodorization of plant extracts, biological enzyme
Effect, quick, lasting feature can effectively remove odorant caused by the fields such as rubbish.
Detailed description of the invention
Fig. 1 is two reaction process schematic diagram of reaction principle in the present invention.
Specific embodiment
A kind of novel safe biologic deodorant and preparation method thereof of the description present invention with reference to embodiments, these descriptions
It is not to be further limited to the content of present invention.
Embodiment 1:
Beneficial to the quality proportioning of microbial inoculum, plant extracts, biological enzyme are as follows: 5.5:2:2.4；
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria effective viable bacteria
The ratio between number are as follows: 10:10:5:5:10:10:6；
Green-tea extract, grape seed extract, yucca extract and Celeryseed extract effective mass proportion: 3.1:2:3:2.4；
Aspartic protease and papain quality proportioning are 1:1；
The preparation method of the efficient biological deodorant of new type of safe, comprising the following steps:
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria are cultivated respectively
To plateau, then mixing, which is added in culture medium, obtains total culture medium, at this time in total culture medium microbial inoculum total living bacteria count
It is 108CFU/ml；The formula of culture medium are as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.4；
The deodorization catalyst that total concentration is 50ppm is added into total culture medium again to be uniformly mixed, deodorization catalyst includes aluminum nitrate
And iron chloride, the molar ratio of aluminum nitrate and iron chloride are 2:3；
Then it will be added in total culture medium in above-mentioned steps beneficial to microbial inoculum and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
Embodiment 2:
Beneficial to the quality proportioning of microbial inoculum, plant extracts, biological enzyme are as follows: 5.8:2:2；
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria effective viable bacteria
The ratio between number are as follows: 10:10:5:5:10:10:7；
Green-tea extract, grape seed extract, yucca extract and Celeryseed extract effective mass proportion: 3:2.8:3:2.3；
Aspartic protease and papain quality proportioning are 1:1.5；
The preparation method of the efficient biological deodorant of new type of safe, comprising the following steps:
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria are cultivated respectively
To plateau, then mixing, which is added in culture medium, obtains total culture medium, at this time in total culture medium microbial inoculum total living bacteria count
It is 108CFU/ml；The formula of culture medium are as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.0；
The deodorization catalyst that total concentration is 10ppm is added into total culture medium again to be uniformly mixed, deodorization catalyst includes aluminum nitrate
And iron chloride, the molar ratio of aluminum nitrate and iron chloride are 2:3；
Then it will be added in total culture medium in above-mentioned steps beneficial to microbial inoculum and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
Embodiment 3:
Beneficial to the quality proportioning of microbial inoculum, plant extracts, biological enzyme are as follows: 6:2:2.1；
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria effective viable bacteria
The ratio between number are as follows: 10:10:5:5:10:10:9；
Green-tea extract, grape seed extract, yucca extract and Celeryseed extract effective mass proportion: 3.2:3:3:2；
Aspartic protease and papain quality proportioning are 1:1.2；
The preparation method of the efficient biological deodorant of new type of safe, comprising the following steps:
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria are cultivated respectively
To plateau, then mixing, which is added in culture medium, obtains total culture medium, at this time in total culture medium microbial inoculum total living bacteria count
It is 108CFU/ml；The formula of culture medium are as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.3；
The deodorization catalyst that total concentration is 55ppm is added into total culture medium again to be uniformly mixed, deodorization catalyst includes aluminum nitrate
And iron chloride, the molar ratio of aluminum nitrate and iron chloride are 2:3；
Then it will be added in total culture medium in above-mentioned steps beneficial to microbial inoculum and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
Embodiment 4:
Beneficial to the quality proportioning of microbial inoculum, plant extracts, biological enzyme are as follows: 5.7:2:2.3；
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria effective viable bacteria
The ratio between number are as follows: 10:10:5:5:10:10:7.5；
Green-tea extract, grape seed extract, yucca extract and Celeryseed extract effective mass proportion: 3.5:2.5:3:
2.2；
Aspartic protease and papain quality proportioning are 1:1.4；
The preparation method of the efficient biological deodorant of new type of safe, comprising the following steps:
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria are cultivated respectively
To plateau, then mixing, which is added in culture medium, obtains total culture medium, at this time in total culture medium microbial inoculum total living bacteria count
It is 108CFU/ml；The formula of culture medium are as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.5；
The deodorization catalyst that total concentration is 100ppm is added into total culture medium again to be uniformly mixed, deodorization catalyst includes aluminum nitrate
And iron chloride, the molar ratio of aluminum nitrate and iron chloride are 2:3；
Then it will be added in total culture medium in above-mentioned steps beneficial to microbial inoculum and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
Embodiment 5:
Beneficial to the quality proportioning of microbial inoculum, plant extracts, biological enzyme are as follows: 5.6:2:2.5；
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria effective viable bacteria
The ratio between number are as follows: 10:10:5:5:10:10:8；
Green-tea extract, grape seed extract, yucca extract and Celeryseed extract effective mass proportion: 3.4:2.3:3:
2.5；
Aspartic protease and papain quality proportioning are 1:1.3；
The preparation method of the efficient biological deodorant of new type of safe, comprising the following steps:
Lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria are cultivated respectively
To plateau, then mixing, which is added in culture medium, obtains total culture medium, at this time in total culture medium microbial inoculum total living bacteria count
It is 108CFU/ml；The formula of culture medium are as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.2；
The deodorization catalyst that total concentration is 80ppm is added into total culture medium again to be uniformly mixed, deodorization catalyst includes aluminum nitrate
And iron chloride, the molar ratio of aluminum nitrate and iron chloride are 2:3；
Then it will be added in total culture medium in above-mentioned steps beneficial to microbial inoculum and be uniformly mixed, be finally added and plant in total culture medium
Preservation getting the product is mixed evenly in object extract, biological enzyme.
In order to verify the deodorizing effect for the deodorant that the present invention is prepared, 1-5 of the embodiment of the present invention is prepared
Deodorant carries out following experiment.
1. nitrogen, hydrogen sulfide removal are tested.
The deodorant that 1-5 of the embodiment of the present invention is prepared passes through different aerating system and corresponding treatment conditions
The removal test of ammonia and hydrogen sulfide is carried out in lower, test situation is as shown in table 1 below.
2. Deodorization Experiment
The deodorant that the cell concentration that deodorant is configured to is 5mgmL-1 is prepared in the embodiment of the present invention, aerobic, warm
For degree to carry out shaking table culture at 30 DEG C, anoxic is that the deodorization after being handled under conditions of static gas wave refrigerator for following aspect is real
It tests.
(1) house refuse Deodorization Experiment.
The rubbish (mainly house refuse) that the highly rotten generation stench of a pile is put in the room sealed between one,
Different collecting locations is set around rubbish and carries out sampling and spray deodorant, 15min is disliked before measurement sprinkling and after sprinkling respectively
NH in smelly3、H2S concentration.
Test result shows that deodorant has apparent deodorizing effect, NH to house refuse3Highest removal rate up to 82.98%,
H2The highest removal rate of S is up to 92.33%.
(2) experiment of deodorant purification animal house air effect.
Deodorant is pressed into every m with spraying3Space measures the concentration of ammonia and hydrogen sulfide toward dispenser uniform on ground.Column
5 point samplings in giving up, are averaged, and repeat experiment 5 times.
The result shows that this deodorant can make ammonia and hydrogen sulfide averagely degradation 72.5% and 81.8% respectively in henhouse, interior air is given up
Ammonia and hydrogen sulfide averagely drop to 16.8mgm respectively-3And 3.9mgm-3, can obviously reduce the stink in henhouse.Research shows that
This deodorant is the current purification preferable cleanser of animal house air.
(3) monitoring and antibacterial tests of factory's henhouse principal causative microorganism are cultivated.
The pollution condition of the 5 chicken farm chicken coop air principal causative microorganisms in the ground such as Yang Ling, Baoji, Gaoling is adjusted
It on the basis of looking into, carries out disinfection using this deodorant, compares the variation of principal causative microorganism before and after the processing.
The result shows that: it is significantly reduced in chicken coop air containing pathogenic microorganisms after processing.Total bacterial inactivation rate is up to 86%~94%, large intestine
Bacillus killing rate is up to 88%~99%, and staphylococcus killing rate is up to 87%~94%, and mould killing rate is up to 91%~96%.It is generally acknowledged that chicken
Air-borne bacteria killing rate is given up up to 80% the above are good, we monitor the killing rate that chicken coop air after disinfection contains 3 kinds of main pathogenic bacterias
Up to 86%~94%, it was demonstrated that this deodorant has stronger killing effect to pathogenic bacteria in chicken coop air, reaches expected environmental Kuznets Curves mesh
's.Test result also shows have on an equal basis using deodorant of the invention and the commercially available easy crin of high-efficiency broad spectrum disinfectant simultaneously
Antibacterial effect.
(4) deodorant tests the bacteriostasis, and deodorization of sport footwear.
It tests, the sample has been carried out in laboratory and scene antibacterial in order to test deodorant to the bacteriostasis, and deodorization of sport footwear
Test and Skin Irritation Test.Spray-on process detects micro- deodorant and tests the fungistatic effect of sport footwear；In laboratory test process,
It will be tested after 240 times of deodorant raw medicine that embodiment 1-5 is prepared and dilution respectively, use deodorization in test at the scene
Agent raw medicine carries out spray testing and Skin Irritation Test, test situation are as shown in table 5 below.
Test result shows that the deodorant raw medicine is respectively 93% to the average bacteriostasis rate of staphylococcus aureus and Candida albicans
With 95%, raw medicine is diluted 240 times, to golden yellow coccus bacteriostasis rate average out to 90.95%；To Candida albicans averagely suppression rate
90.90%.Site test results show to be sprayed with original deodorant raw medicine, are to the bacteriostasis rate that is averaged of natural pollution bacterium in shoes
97.06%, Skin Irritation Test shows that the sample is non-stimulated to skin.The product is suitable for the bacteriostasis, and deodorization to footgear.
(5) natural waters such as pond, lake foul sewage water treatment test.
Launch what 1-5 of the embodiment of the present invention was prepared respectively using adding in the foul sewage water in the pond of Chengdu park
The method of deodorant bacterium solution, system evaluation influence of the deodorant bacterium solution to three classes common contaminant removal rate in sewage.As a result
Show: 1. under aerobic condition, deodorant bacterium solution significantly improves suitable additional amount (the V bacterium solution/V dirt of COD of sewage cr removal rate
Water) it is 5/10000-1/1000, amplification is up to 10%；2. deodorant can significant or extremely significant raising sewage NH under aerobic condition4 +-N
Degree of nitration, when deodorant additional amount is 5/1000, effect is best, and amplification is up to 37.62%；Under anaerobic condition, work as additional amount
When for 1/10000-1/1000, can extremely significant enhancing sewage denitrification, the removal rate of NO3--N about improves 14%.3. micro-
When biological antibiotic deodorization fungus strain additional amount is greater than 5/1000, the dephosphorization ability of sewage can be just significantly improved.From test: deodorization
Agent can effectively degrade the organic substance of sewage, reduce the content of nitrogen, phosphorus, increase the transparency of water body, improve bed mud color
And effectively remove peculiar smell.
(6) to the Deodorization Experiment of sanitary sewage
It takes family kitchen to wash rice, wash vegetables, wash the combined sewages such as fish, is placed several days after giving out a foul smell, take the present invention respectively
The injected volume for the bacterium solution various concentration that embodiment 1-5 is prepared carries out deodorizing process, measurement to the different sewage of olfact
Influence of the injected volume of various concentration deodorant to sewage deodorizing effect.Experiment shows: when the injected volume of liquid deodorant is dirt
Water 0.1% when, deodorizing effect is best；And with to different olfact sanitary sewage deodorizing process, the result is that working as the olfacty of sewage
When being worth higher, this is considered as containing more nutriment in sewage to the more significant of the effect of deodorization, makes the activity of added strain
It is higher, and then deodorizing effect is preferable.
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, is pointed out in claim
Product of the present invention constituent, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out ginseng of the present invention
Therefore several ranges any change within the meaning and scope equivalent to the claims of the present invention, is all considered to be
It is included within the scope of the claims.
The present invention is created by multidigit deodorant processing technology personnel's long-term work experience accumulation, and by creative work
Make and go out, the deodorant that the present invention is prepared has good deodorizing effect, safe and efficient, can suitable for various places
Harm of the foul smell to human body and environment is reduced, so that environmental quality is obviously improved, application value with higher.
2. Deodorization Experiment
The deodorant that the cell concentration that deodorant is configured to is 5mgmL-1 is prepared in the embodiment of the present invention, good
Oxygen, temperature are to carry out shaking table culture at 30 DEG C, and anoxic is removing for following aspect after being handled under conditions of static gas wave refrigerator
Smelly experiment.
(1) house refuse Deodorization Experiment.
The rubbish (mainly house refuse) that the highly rotten generation stench of a pile is put in the room sealed between one,
Different collecting locations is set around rubbish and carries out sampling and spray deodorant, 15min is disliked before measurement sprinkling and after sprinkling respectively
NH in smelly3、H2S concentration.
2 house refuse Deodorization Experiment situation of table
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| NH3Removal rate, % | 82.9 | 82.8 | 82.9 | 82.5 | 82.7 |
| H2S removal rate, % | 92.33 | 92.31 | 92.29 | 92.30 | 92.28 |
Test result shows that deodorant has apparent deodorizing effect, NH to house refuse3Highest removal rate is reachable
82.98%, H2The highest removal rate of S is up to 92.33%.
(2) experiment of deodorant purification animal house air effect.
Deodorant is pressed into every m with spraying3Space measures the concentration of ammonia and hydrogen sulfide toward dispenser uniform on ground.Column
5 point samplings in giving up, are averaged, and repeat experiment 5 times.
The experimental conditions of 3 deodorant of table purification animal house air effect
The result shows that this deodorant can make ammonia and hydrogen sulfide averagely degradation 72.5% and 81.8% respectively in henhouse, house
Interior air ammonia and hydrogen sulfide averagely drop to 16.8mgm respectively-3And 3.9mgm-3, can obviously reduce the stink in henhouse.It grinds
Study carefully and show, this deodorant is the current purification preferable cleanser of animal house air.
(3) monitoring and antibacterial tests of factory's henhouse principal causative microorganism are cultivated.
The pollution condition of the 5 chicken farm chicken coop air principal causative microorganisms in the ground such as Yang Ling, Baoji, Gaoling is adjusted
It on the basis of looking into, carries out disinfection using this deodorant, compares the variation of principal causative microorganism before and after the processing.
The monitoring and antibacterial tests situation of the cultivation factory's henhouse principal causative microorganism of table 4
The result shows that: it is significantly reduced in chicken coop air containing pathogenic microorganisms after processing.Total bacterial inactivation rate up to 86%~
94%, Escherichia coli killing rate up to 88%~99%, staphylococcus killing rate up to 87%~94%, mould killing rate up to 91%~
96%.It is generally acknowledged that chicken coop air bacterial inactivation rate up to 80% the above are good, chicken coop air is containing 3 kinds of masters after we monitor disinfection
Want the killing rate of pathogenic bacteria up to 86%~94%, it was demonstrated that this deodorant has stronger killing effect to pathogenic bacteria in chicken coop air,
Reach expected environmental Kuznets Curves purpose.Test result also shows to sterilize using deodorant of the invention and commercially available high-efficiency broad spectrum simultaneously
The easy crin of medicine has same antibacterial effect.
(4) deodorant tests the bacteriostasis, and deodorization of sport footwear.
It tests, the sample has been carried out in laboratory and scene antibacterial in order to test deodorant to the bacteriostasis, and deodorization of sport footwear
Test and Skin Irritation Test.Spray-on process detects micro- deodorant and tests the fungistatic effect of sport footwear；In laboratory test process,
It will be tested after 240 times of deodorant raw medicine that embodiment 1-5 is prepared and dilution respectively, use deodorization in test at the scene
Agent raw medicine carries out spray testing and Skin Irritation Test, test situation are as shown in table 5 below.
Bacteriostasis, and deodorization test situation of 5 deodorant of table to sport footwear
Test result shows that the deodorant raw medicine distinguishes the average bacteriostasis rate of staphylococcus aureus and Candida albicans
For 93% and 95%, raw medicine is diluted 240 times, to golden yellow coccus bacteriostasis rate average out to 90.95%；It is average to Candida albicans
Suppression rate 90.90%.Site test results show to be sprayed with original deodorant raw medicine, averagely press down to natural pollution bacterium in shoes
Bacterium rate is 97.06%, and Skin Irritation Test shows that the sample is non-stimulated to skin.The product is suitable for removing the antibacterial of footgear
It is smelly.
(5) natural waters such as pond, lake foul sewage water treatment test.
Launch what 1-5 of the embodiment of the present invention was prepared respectively using adding in the foul sewage water in the pond of Chengdu park
The method of deodorant bacterium solution, system evaluation influence of the deodorant bacterium solution to three classes common contaminant removal rate in sewage.As a result
Show: 1. under aerobic condition, deodorant bacterium solution significantly improves the suitable additional amount (V bacterium solution/V sewage) of COD of sewage cr removal rate
For 5/10000-1/1000, amplification is up to 10%；2. deodorant can significant or extremely significant raising sewage NH under aerobic condition4 +- N's
Degree of nitration, when deodorant additional amount is 5/1000, effect is best, and amplification is up to 37.62%；Under anaerobic condition, when additional amount is
When, the denitrification of the extremely significant enhancing sewage of energy, the removal rate of NO3--N about improves 14%.3. micro- life
When object antibacterial and deodouring fungus strain additional amount is greater than 5/1000, the dephosphorization ability of sewage can be just significantly improved.From test: deodorant
Can effectively be degraded the organic substance of sewage, reduce the content of nitrogen, phosphorus, increase the transparency of water body, improve bed mud color simultaneously
And effectively remove peculiar smell.
(6) to the Deodorization Experiment of sanitary sewage
It takes family kitchen to wash rice, wash vegetables, wash the combined sewages such as fish, is placed several days after giving out a foul smell, take this respectively
The injected volume for the bacterium solution various concentration that inventive embodiments 1-5 is prepared carries out deodorizing process to the different sewage of olfact,
Measure influence of the injected volume of various concentration deodorant to sewage deodorizing effect.Experiment shows: when the injected volume of liquid deodorant
For sewage 0.1% when, deodorizing effect is best；And with to different olfact sanitary sewage deodorizing process, the result is that working as sewage
Olfact it is higher when, the more significant of the effect of deodorization this be considered as making added strain containing more nutriment in sewage
Activity it is higher, and then deodorizing effect is preferable.
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, is pointed out in claim
Product of the present invention constituent, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out ginseng of the present invention
Therefore several ranges any change within the meaning and scope equivalent to the claims of the present invention, is all considered to be
It is included within the scope of the claims.
The present invention is created by multidigit deodorant processing technology personnel's long-term work experience accumulation, and by creative work
Make and go out, the deodorant that the present invention is prepared has good deodorizing effect, safe and efficient, can suitable for various places
Harm of the foul smell to human body and environment is reduced, so that environmental quality is obviously improved, application value with higher.
Claims (8)
1. a kind of efficient biological deodorant of new type of safe, it is characterised in that: including beneficial bacterium agent, plant extracts and biology
Enzyme；The beneficial microbial inoculum by lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and
Photosynthetic bacteria composition；The plant extracts is by green-tea extract, grape seed extract, yucca extract and Celeryseed extract
Composition；The biological enzyme is hydrolysising protease；The quality proportioning of the beneficial microbial inoculum, plant extracts, biological enzyme are as follows:
5.5-6:2:2-2.5；The lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and light
The ratio between living bacteria count of combined bacteria are as follows: 10:10:5:5:10:10:6-9, the green-tea extract, grape seed extract, yucca mention
Object and Celeryseed extract effective mass is taken to match: 3-3.5:2-3:3:2-2.5；The beneficial microbial inoculum is dissolved in total culture medium
In, then plant extracts and biological enzyme are added in total culture medium again and are uniformly mixed preservation.
2. the efficient biological deodorant of new type of safe according to claim 1, it is characterised in that: in total culture medium
Total living bacteria count of microbial inoculum is 108CFU/ml。
3. the efficient biological deodorant of new type of safe according to claim 1, it is characterised in that: the biological enzyme is by day
Aspartic acid protease and papain composition.
4. the efficient biological deodorant of new type of safe according to claim 3, it is characterised in that: the aspartic acid albumen
Enzyme and papain quality proportioning are 1:1-1.5.
5. a kind of method for preparing the efficient biological deodorant of new type of safe described in claim 1, it is characterised in that: including with
Lower step:
Step 1) divides lactic acid bacteria, bacillus subtilis, bacillus megaterium, filamentous fungi, actinomyces, saccharomycete and photosynthetic bacteria
Pei Yang not be to plateau, then mixing, which is added in culture medium, obtains total culture medium；
Step 2 will be added in total culture medium in step 1) again beneficial to microbial inoculum to be uniformly mixed, and is finally added in total culture medium
Preservation getting the product is mixed evenly in plant extracts, biological enzyme.
6. a kind of method for preparing the efficient biological deodorant of new type of safe according to claim 5, it is characterised in that: match
The formula of the system culture medium is as follows: beef extract 12g, glucose 10g, peptone 12g, KH2PO42.5g, NaCl5g, water
1000ml, pH value adjustment to 6.0-6.5.
7. a kind of method for preparing the efficient biological deodorant of new type of safe according to claim 5, it is characterised in that: institute
The deodorization catalyst for being 10-100ppm containing total concentration in the total culture medium stated.
8. a kind of method for preparing the efficient biological deodorant of new type of safe according to claim 7, it is characterised in that: institute
The deodorization catalyst stated includes aluminum nitrate and iron chloride, and the molar ratio of the aluminum nitrate and iron chloride is 2:3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910423864.7A CN110052159A (en) | 2019-05-21 | 2019-05-21 | A kind of new type of safe high-performance bio deodorant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910423864.7A CN110052159A (en) | 2019-05-21 | 2019-05-21 | A kind of new type of safe high-performance bio deodorant and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110052159A true CN110052159A (en) | 2019-07-26 |
Family
ID=67323805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910423864.7A Pending CN110052159A (en) | 2019-05-21 | 2019-05-21 | A kind of new type of safe high-performance bio deodorant and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110052159A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111621372A (en) * | 2020-06-01 | 2020-09-04 | 龙秋荣 | Multifunctional plant cleaning deodorant and production method thereof |
| CN111841302A (en) * | 2020-07-30 | 2020-10-30 | 科里思特(福建)生物科技有限公司 | Application method of bacillus subtilis in indoor formaldehyde removal |
| CN111847667A (en) * | 2020-08-04 | 2020-10-30 | 武汉世纪瑞科环保科技有限公司 | Microbial deodorant and preparation method thereof |
| CN112661368A (en) * | 2020-12-14 | 2021-04-16 | 河海大学 | Livestock and poultry excrement deodorant and application thereof |
| CN112795498A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Deodorant for deodorizing and purifying water and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267282A (en) * | 2016-08-25 | 2017-01-04 | 南丹县环境卫生管理站 | A kind of garbage deodorant |
| CN107617331A (en) * | 2017-10-10 | 2018-01-23 | 默克环保科技（湖南）有限公司 | A kind of environment friendly biological deodorant and preparation method thereof |
| CN109718657A (en) * | 2019-02-27 | 2019-05-07 | 山东信得科技股份有限公司 | A kind of galenical deodorizer and preparation method and application method for farm |
-
2019
- 2019-05-21 CN CN201910423864.7A patent/CN110052159A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267282A (en) * | 2016-08-25 | 2017-01-04 | 南丹县环境卫生管理站 | A kind of garbage deodorant |
| CN107617331A (en) * | 2017-10-10 | 2018-01-23 | 默克环保科技（湖南）有限公司 | A kind of environment friendly biological deodorant and preparation method thereof |
| CN109718657A (en) * | 2019-02-27 | 2019-05-07 | 山东信得科技股份有限公司 | A kind of galenical deodorizer and preparation method and application method for farm |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112795498A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Deodorant for deodorizing and purifying water and preparation method thereof |
| CN111621372A (en) * | 2020-06-01 | 2020-09-04 | 龙秋荣 | Multifunctional plant cleaning deodorant and production method thereof |
| CN111841302A (en) * | 2020-07-30 | 2020-10-30 | 科里思特(福建)生物科技有限公司 | Application method of bacillus subtilis in indoor formaldehyde removal |
| CN111847667A (en) * | 2020-08-04 | 2020-10-30 | 武汉世纪瑞科环保科技有限公司 | Microbial deodorant and preparation method thereof |
| CN112661368A (en) * | 2020-12-14 | 2021-04-16 | 河海大学 | Livestock and poultry excrement deodorant and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110052159A (en) | A kind of new type of safe high-performance bio deodorant and preparation method thereof | |
| KR101260872B1 (en) | Composition for removing bad stock raising comprising microorganism probiotics as effective ingredient | |
| CN102258796A (en) | Microbial deodorant and preparation method thereof | |
| CN107162652B (en) | Odorless livestock and poultry manure organic fertilizer and preparation method and application thereof | |
| KR101728942B1 (en) | Deodorizing agent using bacteria and method for manufacturing same | |
| KR102244422B1 (en) | Manufacturing method of natural deodorant containing complex microorganism and the natural deodorant prepared therefrom | |
| KR101512796B1 (en) | Composition for removing an ammonia and a bad smell and method for removing an ammonia and a bad smell using the same | |
| KR101605542B1 (en) | manufacturing method for compost using livestocks' excrements and method for growing plant | |
| CN109279953A (en) | A kind of production method of odorless, high nitrogen livestock manure fermented organic fertilizer | |
| CN101954105A (en) | Method for removing odor of feces and urine of animals and poultry through oxidation | |
| CN106822969A (en) | Novel fermentation becomes thoroughly decomposed deodorant and its application method | |
| KR20190011066A (en) | Natural Microbial agents for Removal of Bad Smell | |
| CN105255761B (en) | A kind of deodorization microbial inoculum and its application method for organic waste treatment | |
| CN106982861A (en) | A kind of biologic garbage bacteria remover and preparation method thereof | |
| KR102035097B1 (en) | Manufacturing method of natural deodorant containing complex microorganism | |
| CN108611301B (en) | Composite biological deodorant and preparation method thereof | |
| CN103656712B (en) | A kind of seaweed bio deodorizer and preparation method thereof | |
| CN109092048A (en) | Microbiocidal deodorant | |
| CN112191085B (en) | Preparation method of garbage deodorant | |
| Paluszak et al. | Sanitization efficacy of anaerobic digestion and aeration of slurry from the aspect of limiting emission of Salmonella into the environment | |
| CN112522159A (en) | Microbial compound microbial inoculum for treating peculiar smell of garbage throwing point | |
| KR100293557B1 (en) | A Deodorizing Agent | |
| CN111197011A (en) | Bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of livestock farm | |
| JP5167534B2 (en) | Medium high temperature sulfur-oxidizing bacteria and method for removing hydrogen sulfide using the bacteria | |
| CN116606783B (en) | Composite microbial agent and application thereof in livestock and poultry cultivation deodorization field |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190726 |
|
| RJ01 | Rejection of invention patent application after publication |