CN110050790B - Application of ketone in attracting diaphorina citri and diaphorina citri attractant - Google Patents
Application of ketone in attracting diaphorina citri and diaphorina citri attractant Download PDFInfo
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- CN110050790B CN110050790B CN201910417276.2A CN201910417276A CN110050790B CN 110050790 B CN110050790 B CN 110050790B CN 201910417276 A CN201910417276 A CN 201910417276A CN 110050790 B CN110050790 B CN 110050790B
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- diaphorina citri
- ketone
- solution
- cyclohexenone
- diaphorina
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
Abstract
The invention relates to the field of plant disease and insect pest control, and provides an application of ketone in attracting diaphorina citri and a diaphorina citri attractant. 2-cyclohexenone and levo-carvone are volatile compounds, the volatile unique smell of the compounds can attract diaphorina citri, and the high-efficiency attraction to the diaphorina citri can be realized without adding other attraction substances; the diaphorina citri attractant provided by the invention comprises ketone and a solvent, and has the advantages of good attracting effect on diaphorina citri, long acting time and convenience in use. The results of the examples show that the induction efficiency of 2-cyclohexenone induced diaphorina citri can reach 44.97% under the condition of volume concentration of 1%, and the induction efficiency of l-carvone induced diaphorina citri can reach 53.27% under the condition of volume concentration of 1%.
Description
Technical Field
The invention relates to the technical field of plant disease and pest control, in particular to application of ketone in luring diaphorina citri and a diaphorina citri attractant.
Background
The diaphorina citri (diaphorin itri Kuwym) is a main pest in the young shoot stage of citrus fruit trees and also is a main transmission medium of pathogen of yellow shoot, most adults lay eggs on young shoots of hosts, and young shoot juice is absorbed after nymphs hatch until the adults eclosion. The damaged tender shoot of the host can be withered, deformed and the like. The psyllids also secrete white honeydew which adheres to branches and leaves and can cause soot diseases.
For the control of diaphorina citri, various methods have been established at present, wherein chemical control mainly uses pesticides, and diaphorina citri can be effectively controlled by actively applying pesticides, but the method causes various problems of drug resistance, environmental pollution, pesticide residue, natural enemies harm and the like. Therefore, many environmentally friendly biological control methods for diaphorina citri have been developed, such as essential oils, plant secondary metabolites, parasitic wasps and entomopathogenic fungi, and yellow armyworm plates.
Phytophagous insects feed by identifying the characteristic odors of secondary metabolites contained in the host plant, which are the signal molecules that the insect seeks out of the host plant. The special odors are fully utilized to attract the diaphorina citri so as to achieve the effect of prevention and treatment, the use of a large amount of pesticides can be reduced, and the drug resistance of pests is reduced. However, the compounds currently used to attract diaphorina citri are still rare.
Disclosure of Invention
In view of the above, the present invention aims to provide an application of ketone in attracting diaphorina citri and a diaphorina citri attractant. The invention applies the ketone to attract the diaphorina citri, has good attracting effect, long acting time and convenient use.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an application of ketone in luring diaphorina citri; the ketone is 2-cyclohexenone and/or l-carvone.
Preferably, the ketone is used in the form of a solution; the solvent of the solution is mineral oil.
Preferably, the volume concentration of the ketone in the solution is 0.1-10%.
Preferably, the diaphorina citri is a diaphorina citri imago.
Preferably, the 2-cyclohexenone is used with an insect trapping device or a mythimna plate.
Preferably, when the ketone is used with armyworm yellow board, the application comprises the following steps:
preparing the ketone into a solution, coating the solution on a pest sticking yellow plate, and inducing diaphorina citri; the coating amount of the ketone solution on each armyworm yellow plate is 1-5 mL.
Preferably, the attracting time is more than or equal to 10 min.
Preferably, the application comprises the following steps:
placing the diaphorina citri at the bottom of the container, and shading the container containing the diaphorina citri;
the opening of the container is connected with a flavor source bottle filled with ketone by a rubber tube;
and taking the opening of the container as a boundary, and determining that the diaphorina citri is induced if the diaphorina citri crosses the boundary.
The invention provides a diaphorina citri attractant which comprises ketone and a solvent; the ketone is 2-cyclohexenone and/or l-carvone.
Preferably, the solvent is mineral oil; the weight percentage content of ketone in the diaphorina citri attractant is 0.1-10%.
The invention provides an application of ketone in attracting diaphorina citri.2-cyclohexenone and levo-carvone are volatile compounds, the volatile unique smell of the ketone can play a role in attracting diaphorina citri, and the high-efficiency attraction to the diaphorina citri can be realized without adding other attraction substances.
The invention also provides a diaphorina citri attractant which comprises ketone and a solvent. The attractant provided by the invention has a good attracting effect on the diaphorina citri, long acting time and convenient use. The results of the examples show that the 2-cyclohexenone is used for inducing the diaphorina citri, the inducing efficiency can still reach 44.97% under the condition that the volume concentration is 1%, and the levo-carvone is used for inducing the diaphorina citri, and the inducing efficiency can still reach 53.27% under the condition that the volume concentration is 1%.
Detailed Description
The invention provides an application of ketone in luring diaphorina citri; the ketone is 2-cyclohexenone and/or l-carvone. In the present invention, the diaphorina citri is preferably an adult diaphorina citri.
In the present invention, the ketone is preferably used in the form of a solution; the solvent of the solution is preferably mineral oil; the volume concentration of the ketone in the solution is preferably 0.1 to 10%, more preferably 0.5 to 9%, further preferably 0.8 to 7%, and most preferably 1%.
In the invention, the attracting time is preferably more than or equal to 10min, more preferably more than or equal to 60 min; in the specific embodiment of the invention, when the induction time is 60min, the induction efficiency of the 2-cyclohexenone on the diaphorina citri can reach 44.97%, and the induction efficiency of the levocarvone on the diaphorina citri can reach 53.27%. In the invention, the 2-cyclohexenone and the levo-carvone are volatile compounds, the volatilized gas has unique smell, can attract the diaphorina citri, is easy to obtain, has low cost and has wide application prospect in the field of controlling the diaphorina citri.
In the present invention, the ketone is preferably used with an insect trapping device or a mythimna panel; when the ketone is preferably used with an insect trapping device, the method of application is specifically: and (3) preparing the ketone into a solution, adding the solution into an insect trapping device, and then trapping the diaphorina citri. When the insect trapping device has a large number of adult diaphorina citri, the ketone solution in the device is preferably replaced. The present invention does not require special requirements for the insect trapping device and uses a trapping tank well known to those skilled in the art.
In the invention, when the ketone and the armyworm yellow plate are used together, the application method specifically comprises the following steps: preparing the ketone into a solution, coating the solution on a pest sticking yellow plate, and inducing diaphorina citri; the coating amount of the ketone solution on each armyworm yellow plate is preferably 1-5 mL, and more preferably 2-3 mL. In the invention, the area of the armyworm yellow plate is preferably 15cm multiplied by 20 cm; in the specific embodiment of the present invention, it is preferable to appropriately supplement the ketone solution on the mythimna separata yellow plate during the attracting process.
In the embodiment of the invention, the diaphorina citri is preferably lured after the evening, the diaphorina citri activity is stronger at night, and the luring effect is better.
In another aspect of the invention, the application preferably comprises the steps of:
placing the diaphorina citri at the bottom of the container, and shading the container containing the diaphorina citri;
the opening of the container is connected with a flavor source bottle filled with ketone by a rubber tube;
and taking the opening of the container as a boundary, and determining that the diaphorina citri is induced if the diaphorina citri crosses the boundary.
The specific manner of the light-shielding treatment in the present invention is not particularly limited, and a manner known to those skilled in the art may be used. In the present invention, the size and material of the container are not particularly limited, and a four-neck flask may be used. The diameter and the length of the rubber tube are not specially limited, and the ketone can be volatilized into the container. In a particular embodiment of the invention, this method of application can be used in a laboratory.
The invention also provides a diaphorina citri attractant which comprises ketone and a solvent; the ketone is 2-cyclohexenone and/or levocarvone; the types of the solvents and the concentrations of the ketones are consistent with the scheme, and are not described again; the application method of the diaphorina citri attractant is consistent with the scheme, and is not described in detail herein.
The embodiments of the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
Example 1
(1) Connecting taste source bottles filled with 2-cyclohexenone solutions with different concentrations respectively at different bottle necks of the four-neck flask by using rubber tubes with the length of 30 cm; the 2-cyclohexenone solution is prepared by using mineral oil, and the volume concentration is 0%, 0.1%, 1% and 10%, wherein 0% is blank control (namely mineral oil);
(2) shading the four-neck flask containing the psyllids, putting about 40 adults at the bottom of the flask at one time, regarding a single-neck bottle passing through the four-neck flask as a boundary, regarding the psyllids passing through the boundary as being induced, recording the quantity of the citrus psyllids at each bottle mouth and the total quantity of the citrus psyllids at four bottle mouths every 10 minutes, and calculating the induction efficiency. The experiment was repeated three times with the unused psyllid replaced for each experiment and the average was taken.
The results are shown in Table 1.
TABLE 1 Induction efficiency of diaphorina citri by different concentrations of 2-cyclohexenone (%)
According to the table 1, the 2-cyclohexenone has an obvious induction effect on the diaphorina citri within the concentration range of 0.1-10%, the 2-cyclohexenone with the concentration of 1% has a better induction effect on the diaphorina citri, and the induction rate can reach 44.97% when the induction time is 60 min.
Example 2
(1) Connecting flavor source bottles filled with levo-carvone solutions with different concentrations respectively at different bottle necks of the four-neck flask by using rubber tubes with the length of 30 cm; the levo-carvone solution is prepared by mineral oil, the volume concentration is respectively 0%, 0.1%, 1% and 10%, wherein 0% is blank control (namely mineral oil);
(2) shading the four-neck flask containing the psyllids, putting about 40 adults at the bottom of the flask at one time, regarding a single-neck bottle passing through the four-neck flask as a boundary, regarding the psyllids passing through the boundary as being induced, recording the quantity of the citrus psyllids at each bottle mouth and the total quantity of the citrus psyllids at four bottle mouths every 10 minutes, and calculating the induction efficiency. The experiment was repeated three times with the unused psyllid replaced for each experiment and the average was taken.
The statistics of the results are shown in Table 2.
Table 2 attraction efficiency (%) -of levocarvone solutions of different volume concentrations on diaphorina citri
According to the table 2, the L-carvone has obvious induction effect on the diaphorina citri in the concentration range of 0.1-10%, the L-carvone with the concentration of 1% has better induction effect on the diaphorina citri, and the induction rate can reach 53.27% when the induction time is 60 min.
Example 3
(1) Connecting areas 1, 2 and 3 of the six-arm olfactometer with a flavor source bottle, leaving the rest 3 arms vacant and closing the arm openings, wherein the area 3 is set as a control area;
(2) opening a vacuum pump, adjusting the airflow speed of each arm of the six-arm olfactometer to 80mL/min, and waiting for the airflow to be stable;
(3) putting 1% 2-cyclohexenone solution (mineral oil as solvent) into the source bottle corresponding to zone 1, putting 1% L-carvone solution (mineral oil as solvent) into the source bottle corresponding to zone 2, and putting equal amount of mineral oil into the source bottle corresponding to zone 3 as control.
Each experimental test was conducted head by head, and the times of the psyllids entering different areas and the times of the areas where the psyllids remained for 3 minutes were recorded as the test results of the tropism reaction of the diaphorina citri to different volatile compounds, and the test results are shown in table 2.
TABLE 3 comparison of the number of selections and the final residence times of 2-cyclohexenone and levocarvone by diaphorina citri
As can be seen from Table 3, both 2-cyclohexenone and l-carvone had good attractant effect against diaphorina citri.
Example 4
In an insect cage with the specification of 75 x 90(cm), an insect trapping experiment is carried out by combining yellow plates (with the specification of 15 x 20cm), and the coating amount of the medicine on each yellow plate is about 1 mL. Respectively coating 1% by volume of levo-carvone (mineral oil as solvent) and 1% by volume of 2-cyclohexenone (mineral oil as solvent) on yellow plates, and suspending the yellow plates together with the yellow plates without medicines in an insect cage for testing; a murraya paniculata is placed in the middle of the insect cage. During the experiment, about 20 psyllids were initially placed, and on the third day, 10 psyllids were placed and 1mL of drug was supplemented. The number of diaphorina citri adults on yellow plates per day was counted, and the experimental results are shown in table 4:
TABLE 41% L-carvone and 1% 2-cyclohexenone Pest Induction results
| Item | Day one | The next day | The third day |
| Control | 4 | 4 | 5 |
| L-carvone | 6 | 7 | 11 |
| 2-Cyclohexenone | 7 | 7 | 10 |
Experimental results prove that the 2-cyclohexenone and the levo-carvone have good attraction effects on the diaphorina citri.
According to the embodiments, the 2-cyclohexenone and the levocarvone have good induction effects on the diaphorina citri and have wide application prospects in the aspect of inducing the diaphorina citri.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. Use of a ketone to attract diaphorina citri, said ketone being 2-cyclohexenone and/or l-carvone.
2. Use according to claim 1, characterized in that the ketone is used in the form of a solution; the solvent of the solution is mineral oil.
3. Use according to claim 2, wherein the ketone is present in the solution in a concentration of 0.1 to 10% by volume.
4. Use according to claim 1, wherein the diaphorina citri is a diaphorina citri adult.
5. Use according to any one of claims 1 to 4, wherein the ketone is used with an insect trapping device or a armyworm mat.
6. Use according to claim 5, wherein when the ketone is used with armyworm yellow board, the use comprises the steps of:
preparing the ketone into a solution, coating the solution on a pest sticking yellow plate, and inducing diaphorina citri; the coating amount of the ketone solution on each armyworm yellow plate is 1-5 mL.
7. The use as claimed in any one of claims 1 to 4, wherein the attracting time is 10min or more.
8. The application according to claim 1, characterized in that it comprises the following steps:
placing the diaphorina citri at the bottom of the container, and shading the container containing the diaphorina citri;
the opening of the container is connected with a flavor source bottle filled with ketone by a rubber tube;
and taking the opening of the container as a boundary, and determining that the diaphorina citri is induced if the diaphorina citri crosses the boundary.
9. A diaphorina citri attractant comprises ketone and solvent; the ketone is 2-cyclohexenone and/or levocarvone; the solvent is mineral oil; the weight percentage content of ketone in the diaphorina citri attractant is 0.1-10%.
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