CN110042039A - A kind of multi-functional sample processing apparatus - Google Patents
A kind of multi-functional sample processing apparatus Download PDFInfo
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- CN110042039A CN110042039A CN201810042126.3A CN201810042126A CN110042039A CN 110042039 A CN110042039 A CN 110042039A CN 201810042126 A CN201810042126 A CN 201810042126A CN 110042039 A CN110042039 A CN 110042039A
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- steel column
- magnet steel
- electric device
- processing apparatus
- sample
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
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Abstract
The present invention provides a kind of multi-functional sample processing apparatus, including controller and support base, and the support base top is equipped with syringe pump and electric device 1.Magnet steel column is installed on the electric device 1, the magnet steel column can be driven to do linear reciprocating motion.Magnet steel column lower end is equipped with the suction nozzle that can flexibly load and unload.The magnet steel column is connect with the syringe pump.The support base lower part is equipped with electric device 2 and casing joints, and the electric device 2 can drive described sleeve pipe connector to be rotated.Described sleeve pipe connector lower end is equipped with the casing that can flexibly load and unload.The magnet steel column can freely enter and leave in described sleeve pipe.Multiple work such as the achievable sample-adding of the present invention, mixing, magnetic bead absorption, liquid relief, facilitate the automation for realizing entire sample process process while improving sample process efficiency.
Description
Technical field
The present invention relates to field of biotechnology more particularly to a kind of paramagnetic particle method nucleic acid extraction instruments.
Background technique
Nucleic acid is the core that hereditary information is expressed in genome, is the important object of life science.In molecular diagnosis
With Food Safety Analysis field, following several processes are related to the detection and analysis of nucleic acid, are the extraction target core from sample first
Then acid expands target nucleic acid, finally complete the detection of target nucleic acid.
Wherein the extraction of target nucleic acid is the most important link of entire test experience, be directly related to experimental result at
It loses.And the extraction of target nucleic acid is related to the complex process to sample.The reality of nucleic acid is extracted in the paramagnetic particle method being widely used at present
In testing, the processing of sample is related to the following steps: sample liquid, lysate, magnetic bead are added separately in test tube by the first step；
Second step mixes well the liquid in test tube, and cell is cleaved in sample under the action of lysate, the nucleic acid separated
It realizes and specifically binds with magnetic bead；Bar magnet is inserted into test tube by third step, and magnetic bead is adsorbed on bar magnet；4th step is by nucleic acid and magnetic
Pearl is transferred in cleaning solution and washs；5th step elutes magnetic bead, realizes the separation of nucleic acid and magnetic bead；6th step is by the magnetic after elution
Pearl separation removes, to obtain target nucleic acid.
The process of sample process is related to the work such as multiple sample-adding, mixing, magnetic bead absorption, liquid relief, requires using special
Instrument or equipment complete, such as pipettor, vortex mixer, magnetic frame.Entire sample process Period Process length, low efficiency, at
This height.
Summary of the invention
The present invention addresses the above problem, provides a kind of multi-functional sample processing apparatus, can undertake sample-adding simultaneously, mix
Multiple work such as even, magnetic bead absorption, liquid relief.Reduce the process that detected sample operates between multiple laboratory apparatus, improves
The efficiency of sample process.Facilitate the automation for realizing entire sample process process.
Technical proposal that the invention solves the above-mentioned problems is as follows:
A kind of multi-functional sample processing apparatus, including controller and support base, the support base top are equipped with syringe pump
With electric device 1.Magnet steel column is installed on the electric device 1, the magnet steel column can be driven to do linear reciprocating motion.It is described
Magnet steel column lower end is equipped with the suction nozzle that can flexibly load and unload.The magnet steel column is connect with the syringe pump.The support base lower part peace
Equipped with electric device 2 and casing joints, the electric device 2 can drive described sleeve pipe connector to be rotated.Institute
It states casing joints lower end and the casing that can flexibly load and unload is installed.The magnet steel column can freely enter and leave in described sleeve pipe.
The beneficial effects of the present invention are:
The magnet steel column is hollow structure, and suction nozzle is installed in lower end, and cooperates the syringe pump, can play the function of pipettor
Energy.The magnet steel column itself has very strong magnetism, is inserted into casing, can be used as the bar magnet of absorption magnetic bead.Described sleeve pipe exists
The electric device 2 is rotated by, and can play the function of mixing to sample liquid.It is designed in this way, whole device is integrated
Degree is high, compact-sized, small in size, uses and safeguard simply, at low cost.It is greatly improved the efficiency of sample process.It is easy to implement
Sample process process automation.
Based on the above technical solution, the present invention can also be improved as follows.
Further, the syringe pump is Mini gear pump.
Beneficial effect using above-mentioned further scheme is: utilizing gas-liquid principle,displacement, it is ensured that sample-adding precision is met the requirements
While reduce whole device volume, reduce manufacturing cost.
Further, flexible hose of the magnet steel column with the syringe pump by internal diameter no more than 0.5 millimeter is connect.
Beneficial effect using above-mentioned further scheme is: reducing the volume of connection air in tube, further increases sample
It is loaded precision, while ensuring that the magnet steel column can freely move along a straight line.
Further, the magnet steel column is hollow cylinder, and the diameter of hollow cavity is not more than 0.5 millimeter.
Beneficial effect using above-mentioned further scheme is: reducing the volume of air in magnet steel column, further increases sample
It is loaded precision.
Detailed description of the invention
Fig. 1 is structural front view of the invention；
Fig. 2 is present invention sample-adding status diagram；
Fig. 3 is that the present invention mixes status diagram；
Fig. 4 is magnetic bead adsorbed state schematic diagram of the present invention；
In Fig. 1,1, support base, 2, upper stopper, 3, motor cabinet first, 4, spindle motor, 5, sliding block, 6, casing joints,
7, casing joints support, 8, driven gear, 9, driving gear, 10, motor, 11, motor cabinet second, 12, magnet steel column, 13, injection
Pump, 14, hose
In Fig. 2,15, suction nozzle
In Fig. 3,16, casing
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
As shown, a kind of multi-functional sample processing apparatus, including controller and support base 1,1 top of the support base peace
Equipped with upper stopper 2 and motor cabinet first 3, lead screw motor 4 and sliding block 5 are installed between the upper stopper 2 and motor cabinet first 3.It is described
Magnet steel column 12 is installed on sliding block 5.12 lower end of magnet steel column can flexibly load and unload suction nozzle 15.1 top of the support base installation
There is syringe pump 13, the syringe pump 13 is connect with the magnet steel column 12 by hose 14.1 lower part of support base is equipped with motor
Seat second 11, is equipped with motor 10 in the motor cabinet second 11, and driving gear 9 is mounted in the shaft of the motor 10, and with from
Moving gear 8 engages.The driven gear 8 is fixed on casing joints 6.1 lower part of support base is equipped with casing joints
Support 7, described sleeve pipe connector 6 are mounted in casing joints support 7, can flexibly load and unload casing 16.
Specifically, the syringe pump 13 is Mini gear pump.
Specifically, flexible hose of the magnet steel column 12 with the syringe pump 13 by internal diameter no more than 0.5 millimeter is connect.
Specifically, the magnet steel column 12 is hollow cylinder, the diameter of hollow cavity is not more than 0.5 millimeter.
Specifically, the controller is industrial single-chip microcontroller, the drive control realized to the motor and syringe pump is concentrated.
The controller is connect with computer by usb signal line.
Specific operation process of the present invention is as follows:
1, sample, lysate, magnetic bead, cleaning solution, de- washing lotion are got out on work top.
2, the spindle motor 4 is moved down with movable slider 5, and then magnet steel column 12 is driven to move down.Magnet steel column 12 is worn
Casing joints 6 are crossed, stop motion when reaching least significant end.
3, new suction nozzle 15 is installed in 12 lower end of magnet steel column, and the present apparatus is moved at lysate, and syringe pump 13 is started to work, will
Lysate is drawn into suction nozzle 15.
4, the present apparatus is moved at sample, and the liquid in suction nozzle 15 is added in sample syringe pump 13.
5, above step 2 to 3 is repeated, magnetic bead is added in sample.
6, the suction nozzle 15 on magnet steel column 12 is laid down, spindle motor 4 is moved up with movable slider 5, and then drives magnet steel column 12 upward
It is mobile.Magnet steel column 12 passes through casing joints 6, stop motion when reaching most significant end.
7, new casing 16 is installed in 6 lower end of casing joints, and is inserted into casing 16 in sample.Motor 10 starts work
Make, be driven by gear assembly, casing joints 6 is driven to rotate, and then drives casing 16 to rotate and sample liquid is mixed
It is even.Cell is cleaved in sample under the action of lysate, and the nucleic acid separated and magnetic bead are realized and specifically bound.It is sufficiently mixed
After even, motor 10 stops operating.
8, spindle motor 4 is moved down with movable slider 5, and then magnet steel column 12 is driven to move down.Magnet steel column 12 passes through set
Pipe connections 6 enter in casing 16, stop motion when reaching least significant end.
9, under the magneticaction of magnet steel column 12, the magnetic bead in test tube is adsorbed on the outer wall of casing 16.
10, the present apparatus is moved at cleaning solution, and the casing 16 for being adsorbed with magnetic bead is inserted into cleaning solution.Spindle motor 4 drives
Sliding block 5 moves up, and then magnet steel column 12 is driven to move up.Magnet steel column 12 passes through casing joints 6 and leaves casing 16, reaches most
Stop motion when high-end.
11, motor 10 is started to work, and is driven by gear assembly, and casing joints 6 is driven to rotate, and then drives casing 16
Rotation, is followed by stirring and washing magnetic bead and the nucleic acid of specific binding.
12, sufficiently after washing, repeat above step 8 to 9.By magnetic bead and the nucleic acid absorption of specific binding to casing 16
Outer wall.
13, the present apparatus is moved at de- washing lotion, repeats above step 10 to 12.De- washing lotion separates magnetic bead with nucleic acid, magnetic
After integument is adsorbed onto 16 outer wall of casing, it is dropped together together with casing 16.Nucleic acid in remaining liquid to extract.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (4)
1. a kind of multi-functional sample processing apparatus, it is characterised in that: including controller and support base, the support base top installation
There are syringe pump and electric device 1.Magnet steel column is installed on the electric device 1, the magnet steel column can be driven to do reciprocating linear
Movement.Magnet steel column lower end is equipped with the suction nozzle that can flexibly load and unload.The magnet steel column is connect with the syringe pump.The support
Seat lower part is equipped with electric device 2 and casing joints, and the electric device 2 can drive described sleeve pipe connector to be rotated
Movement.Described sleeve pipe connector lower end is equipped with the casing that can flexibly load and unload.The magnet steel column can be in described sleeve pipe freely
It enters and leaves.
2. a kind of multi-functional sample processing apparatus according to claim 1, it is characterised in that: the syringe pump is miniature tooth
Wheel pump.
3. a kind of multi-functional sample processing apparatus according to claim 1, it is characterised in that: the magnet steel column and the note
Flexible hose of the pump by internal diameter no more than 0.5 millimeter is penetrated to connect.
4. a kind of multi-functional sample processing apparatus according to claim 1, it is characterised in that: the magnet steel column is open circles
The diameter of cylinder, hollow cavity is not more than 0.5 millimeter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810042126.3A CN110042039A (en) | 2018-01-16 | 2018-01-16 | A kind of multi-functional sample processing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810042126.3A CN110042039A (en) | 2018-01-16 | 2018-01-16 | A kind of multi-functional sample processing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110042039A true CN110042039A (en) | 2019-07-23 |
Family
ID=67273586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810042126.3A Pending CN110042039A (en) | 2018-01-16 | 2018-01-16 | A kind of multi-functional sample processing apparatus |
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| Country | Link |
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| CN (1) | CN110042039A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114774241A (en) * | 2022-06-22 | 2022-07-22 | 翊新诊断技术(苏州)有限公司 | Magnetic bead extraction device |
Citations (8)
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| CN1535381A (en) * | 2001-05-25 | 2004-10-06 | ������ķϵͳ֪ʶ��Ȩ����˾ | Liquid handling means for excision apparatus |
| CN101596469A (en) * | 2009-07-03 | 2009-12-09 | 烟台艾德康生物科技有限公司 | Multi-channel loading device |
| CN101838609A (en) * | 2010-03-15 | 2010-09-22 | 上海浩源生物科技有限公司 | Sample adding device and application thereof |
| WO2011150833A1 (en) * | 2010-05-31 | 2011-12-08 | 上海仁度生物科技有限公司 | Test tube, device comprising test tube and liquid treatment method by using same |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103881894A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| WO2017067093A1 (en) * | 2015-10-21 | 2017-04-27 | 陈辉 | Nucleic acid extraction apparatus and extraction method |
-
2018
- 2018-01-16 CN CN201810042126.3A patent/CN110042039A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1535381A (en) * | 2001-05-25 | 2004-10-06 | ������ķϵͳ֪ʶ��Ȩ����˾ | Liquid handling means for excision apparatus |
| CN101596469A (en) * | 2009-07-03 | 2009-12-09 | 烟台艾德康生物科技有限公司 | Multi-channel loading device |
| CN101838609A (en) * | 2010-03-15 | 2010-09-22 | 上海浩源生物科技有限公司 | Sample adding device and application thereof |
| WO2011150833A1 (en) * | 2010-05-31 | 2011-12-08 | 上海仁度生物科技有限公司 | Test tube, device comprising test tube and liquid treatment method by using same |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN103881894A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| WO2017067093A1 (en) * | 2015-10-21 | 2017-04-27 | 陈辉 | Nucleic acid extraction apparatus and extraction method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114774241A (en) * | 2022-06-22 | 2022-07-22 | 翊新诊断技术(苏州)有限公司 | Magnetic bead extraction device |
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Application publication date: 20190723 |
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